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FATOS KOPLIKU

Summary of qualifications
Molecular biologist with 8 years of experience in scientific research. Strong an
alytical, organizational and mentoring skills. Accurate and detailed record-keep
ing. Looking for a position with and emphasis on molecular biology and microbiol
ogy research.
Molecular Biology and Microbiology
* Expertise with modern techniques in molecular biology like:
DNA/RNA extraction Cloning
PCR, Q-PCR Ribotyping
cDNA library Sequencing
RLB Hybridization Assay Agarose Gel Electrophoresis
* Proficient at working in BSL1-2 level environment.
* Expertise in in vitro growth of cell cultures using enriched or selective medi
a in aerobic and anaerobic conditions.
* Colony isolation and growth, Inoculation and Incubation, Cell culture, Aseptic
technique.

Automation and Computer


* Expertise with automation set-up like:epMotion 5075 workstation robot, eGene C
apillary Electrophoresis System
CEQ 8000 Genetic Analysis System, Roche Magna Pure robotic workstation
LightCycler 480 RT-PCR System
* Computer skills:
MacClade, Sequencher, PAUP, BLAST, PHYLIP, SPOT, Word, Excel, Powerpoint.

Education
Master of Science. Entomology Department, University of Massachusetts, Amherst,
MA (2008)
Bachelor of Science. Molecular Biology and Genetics, Bogazici University, Istanb
ul, Turkey (2001)

Experience
Research Associate - Medical Center, University of Michigan (2008-present)
o Responsible for the creation of a 6000-sequence database using 16S rDNA clone
library approach to assess the diversity of microbial community in mouse intesti
nal tissue.
o Organization and archiving of hundreds of samples from human patients screened
for the presence of Clostridium difficile strains using anaerobic microbiology
and molecular biology methods.
o Follows literature relevant to the ongoing projects. Attendance of weekly meet
ings and reports.
o Mentor of undergraduate and graduate students in a laboratory setting.
o In charge of writing and updating SOP's to improve the efficiency of laborator
y processes.

Graduate Student, Research/Teaching assistant - University of Massachusetts, Amh


erst, MA (2006 - 2008)
o Lead the project focused on assessing the genetic diversity of Borrelia burgdo
rferi (the agent of Lyme disease) in Massachusetts using nested-PCR and hybridiz
ation assay technique.
o Created and organized a 4000-sample collection from extraction of Ixodes scapu
laris (deer-tick) DNA.
o In charge of identifying and analyzing microsatellite repeats in deer tick DNA
using CEQ Genetic Analysis System.
o Successfully performed putative gene knockdown in Trypanosoma brucei (the slee
ping sickness agent) using RNAi technology.
o Responsible for leading and coordinating field-work collecting.
o Teaching assistant and mentor of undergraduate students.
Research Assistant - Brigham Young University, Provo, UT (2004 - 2005)
o Expertly applied cDNA library methodology to assess differential gene expressi
on in various organisms.
o Successfully designed PCR primers for various mitochondrial and nuclear genes
using Sequencher software.
o Expertise with dissection of insects and slide preparation.
Research Assistant - Natural History Museum, London, UK (2002 - 2004)
o Created a 200-sample collection from the extraction of DNA and RNA from leaf b
eetles (Chrysomelidae)
o Extensively and expertly used 16S and 28S genes in PCR and Sequencing.
o PAUP software application to construct phylogenetic relationship between
organisms based on ribosomal DNA sequences of interest.
PERSONAL
o Avid reader, Marathon runner, Member of KidsAfrik soccer team and charity prog
ram.
Publications / Posters
Walk, S. T., F. Kopliku, J. Opp, A. Bloom, J. Weinstock, and V. B. Young. Altera
tion of the murine gut microbiota by the helminth, Heligmosomoides polygyrus, an
d the antihelminthic agent, pyrantel. American Society for Microbiology General
Meeting, Philadelphia, Pennsylvania. (2009)
Gomez-Zurita J, Hunt T, Kopliku F, Vogler AP. Recalibrated Tree of Leaf Beetles
(Chrysomelidae) Indicates Independent Diversification of Angiosperms and Their I
nsect
Herbivores. PLoS ONE 2(4): e360. (2007)
Gomez-Zurita J, Kopliku F, Theodorides K, Vogler AP. Resources for a phylogenomi
c
approach in leaf beetle systematics. New development in the Biology of Chrysomel
idae,
pp19-35. 2004 SPB Academic Publishing, The Hague, The Netherlands
References available upon request.

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