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Fundamental Principles
of Membrane Biophysics
David Njus
Department of Biological Sciences
Wayne State University
© D. Njus, 2000
Fundamental Principles
of Membrane Biophysics
David Njus
© D. Njus, 2000
Table of Contents
Appendices
I. Glossary of Symbols
II. Abbreviations
V. Mathematical Formulae
Glossary of Symbols
A area x distance
C molar concentration, capacitance Z collision factor
c velocity of light z valence
cmc critical micelle concentration
D diffusion coefficient
d derivative γ activity coefficient
E energy, reduction potential ∆ difference
E electric field δ dipole moment
e electronic charge ε dielectric constant
F force εo permittivity of vacuum
F Faraday constant
f frictional coefficient η viscosity, efficiency
f fugacity λ wavelength of light
G Gibbs free energy µ electrochemical potential
g conductance, acceleration of gravity ρ density
H enthalpy σ reflection coefficient
h Planck’s constant ψ electrical potential
I current
J flow
Ki equilibrium constant for reaction i
Kp partition coefficient
k Boltzmann constant
ki rate constant for reaction i
m mass, aggregation number
N Avogadro's number
n amount in moles
P permeability coefficient, pressure, power
Q heat
q charge
R gas constant, resistance
r radius
S entropy
T absolute temperature
t time
u mobility
V volume
v velocity
W work
X mole fraction
Abbreviations
Å Angstroms
atm atmospheres k kilo 103
°C degrees centigrade c centi 10-2
cal calories
m milli 10-3
coul coulombs
D Debyes µ micro 10-6
Da Daltons n nano 10-9
eq equivalents p pico 10-12
esu electrostatic units f femto 10-15
F farads (coul/volt) a atto 10-18
g grams
j joules
°K degrees Kelvin
l liters
M moles/liter
m meters
min minutes
mol moles
S Siemens
sec seconds
V volts
Fundamental Constants
Conversion Factors
Surface Area
Cylinder (minus ends) A = 2πrh
Sphere A = 4πr2
Volume
Cylinder V = πr2h
Sphere V = (4/3) πr3
Fundamental Principles
of Membrane Biophysics
David Njus
© D. Njus, 2000
CHAPTER 1: BIOLOGICAL MEMBRANES
Page 1.1
content was twice that needed for a single layer of lipid led to the concept (correct, as luck
would have it) of the lipid bilayer (Gorter and Grendel, 1925; Danielli and Davson, 1935).
Lipids are amphiphilic compounds with a small hydrophilic headgroup attached to long
hydrocarbon chains. In the lipid bilayer, lipids are aligned with the headgroups facing the
water on either surface of the membrane and the hydrophobic hydrocarbons sandwiched in
between.
The lipid bilayer concept did not establish the location of the protein components of
the membrane. Originally, for lack of a better site, the proteins were stuck on to the
membrane surface. This was not tenable, of course, because proteins are responsible for
moving molecules and messages across membranes and they could not perform those
functions without being a integral part of the membrane itself. This realization gave rise to
the concept of integral and peripheral proteins. Peripheral proteins are loosely associated
with the membrane and located on the surface of the lipid bilayer. Integral proteins are
inserted into the membrane and pass all the way (or much of the way) across the
membrane. Originally, the integral proteins were thought to form a well defined matrix
with the lipid bilayer filling in the spaces in between. In the late 1960's, however, it
became clear that many proteins are not rigidly fixed in the membrane, but can diffuse
across the surfaces of cells relatively easily and independently. Membranes came to be
viewed as fluid structures with proteins and lipids arranged in their thermodynamically
most favorable structure. Lipids exist in a bilayer and provide the milieu in which the
integral membrane proteins float. The proteins are oriented so that their hydrophobic
surfaces are immersed in the hydrophobic interior of the lipid bilayer. Hydrophilic amino
acids are exposed only in the aqueous regions on either side of the membrane. The
organization of the membrane is a direct consequence of the partitioning of its components,
both lipid and protein, so that hydrophobic regions are kept within the membrane and
hydrophilic parts are exposed to the water on either side. Because the components are not
held together by bonds, they are free to diffuse and move independently within the plane of
the membrane. Singer and Nicolson (1972) captured this view in the fluid mosaic model.
The fluid mosaic model persists as the accepted view of membrane structure. The
recognition of linkages between membrane proteins and components of the cytoskeletal
system has modified the concept somewhat. The cytoskeleton does impose some
constraints on the distribution of membrane proteins. As we shall see, phase separation
also can create separate domains with different characteristics within membranes.
Consequently, the fluid mosaic model does not imply that all the components of a
particular membrane are randomly and homogeneously distributed.
Page 1.2
mechanisms, but a redox chain is common. These membranes can use the proton gradient
to generate ATP using an ATP synthase of the F1F0 type.
The second class consists of plasma membranes of animal cells. These have a
Na+/K+ ATPase which pumps Na+ out of and K+ into the cells. The Na+ and K+
concentration gradients created thereby participate in many functions of the membrane
including transport, excitability, and signalling.
The third class of membranes includes the plasma membranes of plant and fungal
cells. These differ from animal cell membranes in that they lack a Na+/K+ ATPase and
instead have a proton-translocating ATPase of the P-type. The proton gradient created by
this ATPase drives transport and other functions of the plant plasma membrane. This
fundamental difference between plant and animal cell membranes reflects a basic
difference between plant and animal lifestyles. Unlike animal cells, plant cells cannot rely
on seawater or a circulatory fluid to provide external Na+, so the substitution of a proton
pump for a sodium pump is necessary. Moreover, because plants are immobile, plant cells
can have a rigid cell wall to support the plasma membrane in times of osmotic imbalance.
Animal cells, by contrast, must maintain osmotic balance by regulating the concentrations
of internal osmolytes such as Na+ and K+ to balance the external salt concentration.
The fourth class of membranes includes membranes of the vacuolar system. This
comprises the membranes of Golgi-derived organelles including lysosomes, endosomes,
secretory vesicles in animal cells and peroxisomes, vacuoles and tonoplasts in plants and
fungi. These membranes have a proton-translocating ATPase of the V-type. The proton
gradient created by this ATPase drives processes in the membrane and also makes the
interior of the organelle acidic, a property frequently important in the function of the
organelle.
Page 1.3
proteins are placed in the membrane with a defined orientation and this gives the
membrane polarity.
The structure of a biological membrane is a consequence of both spontaneous
assembly and programmed development. The fluid mosaic model emphasizes the
spontaneous assembly of the lipid bilayer with the proteins orienting to accommodate their
own hydrophobic and hydrophilic surfaces. At the same time, the membrane is
asymmetrical largely because of its history. The structure of each protein is determined by
the amino acid sequence and the direction in which that sequence was inserted into the
membrane. The further processing of that protein, particularly the attachment of
carbohydrates, is also asymmetrical since the relevant enzymes are confined to one side of
the membrane or the other. The asymmetry is obviously crucial because it gives
membranes a polarity essential for function. Transport occurs in defined directions. This
in turn creates the membrane potential, a polarity that plays a fundamental role in many
membrane processes.
References
S.J. Singer and G.L. Nicolson (1972) The fluid mosaic model of the structure of cell
membranes, Science 175, 720-731.
Page 1.4
Fundamental Principles
of Membrane Biophysics
David Njus
© D. Njus, 2000
CHAPTER 2: THERMODYNAMICS OF MICELLE FORMATION
Page 2.1
Figure 2.1
Page 2.2
Section 2.2. Structures Formed by Amphiphilic Molecules
Amphiphiles are those molecules that are polar on one end and nonpolar on the
other. These promiscuous molecules have affinities for both aqueous and nonpolar phases.
At low concentrations, they dissolve in water. At some critical concentration, however,
they reach their solubility limit and begin to aggregate into micellar structures. The micelle
structure allows the molecule to keep its polar region in the aqueous phase on the surface
of the micelle and the nonpolar portion in the nonpolar interior of the micelle. The limiting
monomer solubility is called the critical micelle concentration (cmc). At concentrations
below the cmc, the amphiphile will exist as monomers. At concentrations above this level,
the excess amphiphile will aggregate to form micelles.
Two factors are involved in the spontaneous formation of micelles. First, the
hydrophobic effect causes the nonpolar portion of the molecule to be separated from water
and sequestered in the interior of the structure. Second, interactions between the head
groups determine how closely the molecules may be packed. Amphiphiles with a single
hydrocarbon chain, such as dodecyl sulfate, must pack a number of head groups around a
relatively small volume of hydrocarbon. This large surface area to volume ratio is achieved
by forming a spherical micelle structure. By contrast, amphiphiles with two hydrocarbon
chains, such as phospholipids, must pack the same number of headgroups around twice as
large a volume of hydrocarbon. This smaller surface area to volume ratio is achieved by
forming the bilayer structure (Figure 2.2).
Figure 2.2
It should be recognized that the spherical micelle and the planar bilayer are really
two extremes of a continuum. Micelles in the shape of oblate spheroids will exhibit
intermediate ratios of surface area to volume. Under a particular set of conditions, an
amphiphile will form micelles with a particular surface area to volume ratio and thus will
form micelles of a particular size. This characteristic of the micelle is described by the
aggregation number m, the average number of amphiphile molecules in a single micelle.
The critical micelle concentration and the aggregation number together characterize
the micelle that a particular amphiphile will form under a given set of conditions. We can
make some intuitive generalizations about how these parameters should respond to a
variety of changes. Increasing the chain length of the amphiphile should lower the aqueous
solubility and decrease the critical micelle concentration. For similar reasons, amphiphiles
with two hydrocarbon chains (phospholipids) should have a lower cmc than those with a
single chain (detergents). Ionic detergents should have a greater water solubility than
nonionic detergents and therefore should have a higher critical micelle concentration.
Repulsive forces between polar groups should be less for nonionic detergents than for ionic
detergents. Therefore, nonionic detergents should form micelles with smaller surface areas
per amphiphile (larger aggregation numbers). Increasing the ionic strength should diminish
Page 2.3
repulsive forces between polar groups of ionic detergents thereby increasing the
aggregation number. These characteristics of the cmc and the aggregation number are
illustrated by the data in Table I. Deoxycholate, cholate and sodium dodecyl sulfate are
ionic detergents; Lubrol WX and Triton X-100 are nonionic.
TABLE 2.1
(2.1) µw = µw° + RT ln Xw + RT ln fw
Since Xw is the saturating concentration (in mole fraction) of the hydrocarbon in water, the
free energy change for transferring a hydrocarbon molecule from water into the
hydrocarbon phase can be determined from the compound's water solubility:
Page 2.4
(2.4) µHC° - µw° = RT ln Xw
where nc is the number of carbon atoms in the molecule. Of course, as nc increases, the
water solubility of the hydrocarbon decreases. Double bonds increase the water solubility
(decrease the hydrophobicity) of hydrocarbons.
We can use a similar analysis to examine the partitioning of hydrocarbon molecules
between water and the interior of a micelle. The chemical potential of the hydrocarbon
molecule in a micelle is
Setting µmic equal to the chemical potential of the hydrocarbon in water (equation 2.1)
allows us to solve for the free energy change for transfer of the hydrocarbon from water to
the interior of the micelle:
If Xw is the solubility of the hydrocarbon in water, then Xmic can be calculated from the
increase in solubility observed in the presence of micelles. For a series of n-alkanes and
micelles formed from sodium dodecyl sulfate, the following empirical relationship is
observed:
(2.8) µmic° - µw° = -1934 - 771 nc cal/mol
As in the case of the transfer of hydrocarbon from water to the pure hydrocarbon phase, the
free energy change is proportional to the number of carbon atoms in the compound and the
energy contribution of each carbon atom is about 800 cal/mol.
(2.9) µw = µw° + RT ln Xw + RT ln fw
and
(2.10) µmic,m = µmic,m° + (RT/m) ln (Xm/m)
RT ln (Xm/m) is the contribution of the whole micelle to the free energy, so this term is
divided by m to determine the free energy contribution of each molecule of amphiphile.
Page 2.5
Since amphiphiles will equilibrate between the aqueous phase and micelles, these chemical
potentials must be equal. Therefore,
This expression attributes 700 cal/mol to each carbon atom in the hydrocarbon chain in
rough agreement with the values given in Section 2.3. The hydrophobic effect is also
assumed to depend linearly on the surface area of the micelle per molecule of amphiphile
(A). A is measured in square angstroms, so each square angstrom of hydrophobic surface
area per molecule adds about 25 cal/mol to the free energy.
This dependence of the free energy on micelle surface area per molecule is crucial
since it helps determine the size of the micelle. While the volume of the micelle increases
in proportion to the aggregation number m, the surface area increases only in proportion to
m2/3. Therefore, larger micelles will have a smaller surface area per molecule and smaller
micelles will have a larger surface area per molecule. Under a given set of conditions, an
amphiphile molecule will have an optimum surface area, and this will determine the size of
the micelle that it will form.
Having estimated the contribution of the hydrophobic effect to the free energy of
micelle formation, we must estimate the contribution of the head group interaction (Wm).
This may be calculated from pressure vs. area curves measured for monolayers of
amphiphile. If an amphiphile is added to water, it will form a monolayer on the surface of
the water with the polar end in the water and the nonpolar region extending into the air.
Page 2.6
The pressure required to compress this monolayer will depend on the repulsive forces
between the head groups (Phg) and on the intrinsic pressure exerted by ideal molecules by
virtue of their kinetic energy (Pke). The latter pressure is
where A is the surface area per molecule and k is the Boltzmann constant. This expression
is the two-dimensional correlate of the ideal gas law. The pressure attributable to head
group interaction is therefore
(2.18) Wm = - ∫ (P-kT/A) dA
This work function can be evaluated by integrating pressure vs. area curves determined
from monolayer compression experiments and corrected for kT/A (Figure 2.2). For the
trimethylammonium and sulfate head groups, the work functions as evaluated by Tanford
are
50
P (erg/cm 2 )
40
30
Ptotal
20
Phg
10
0
40 60 80 100
A (Å 2 )
Figure 2.3
We now have semiempirical expressions that can be used to calculate the free
energies involved in the formation of dodecyl sulfate and cetyltrimethylammonium
Page 2.7
micelles. Table II compares experimental results to results of Tanford's semiempirical
calculations based on equations 2.12 and 2.13. If head group interactions are calculated
theoretically (Debye-Huckel theory), agreement with experimental results is not as good.
TABLE 2.2
m cmc (M)
_________________________________________________________
Experimental
N+(CH3)3 59 0.0066
OSO3- 95 0.0015
Semiempirical (Monolayer data)
N+(CH3)3 60 0.0062
-
OSO3 93 0.0018
Theoretical (Debye-Huckel)
OSO3- 39 0.0042
_________________________________________________________
The hydrophobic effect and the head group interaction have both been cast as
functions of the micelle surface area per molecule (equations 2.15, 2.19 and 2.20). It is
instructive to plot these energies as functions of the surface area (Figure 2.4). This shows
that there is a molecular surface area which minimizes the total free energy. The surface
area giving this minimum free energy determines the aggregation number of the micelle. A
larger surface area per molecule implies smaller micelles. A smaller surface area per
molecule implies larger micelles with the bilayer structure being the limiting case
(infinitely large micelle). The minimum free energy itself is RT ln cmc (equation 2.12).
This plot illustrates effects of changes in the energies. For example, decreasing the
work function by increasing the ionic strength will lower the cmc and increase the
aggregation number (Figure 2.5).
Page 2.8
4
Wm
µ (kcal/mol) 2
-2
-4 U° mic,m - µ°w
RT ln cmc
-6
U°m - µ°w
-8
50 75 A min 100
larger smaller
micelles 2 micelles
A (Å )
Figure 2.4
U° mic,m - µ°w
U° m- µ° w
-10
50 100
A (Å 2 )
Figure 2.5
Page 2.9
Lauryl Sulfate (Dodecyl sulfate)
-
O 3S O CH2 (CH2 )10 CH 3
Cetyltrimethylammonium (CTAB)
CH 3
CH 3
Cholic Acid: X = OH
Deoxycholic Acid: X = H
CH 3
OH
CH 3
-
OOC
H CH 3
H H
7
X OH
H
Page 2.10
Triton X-100 (Octylphenoxypolyoxyethanol)
Lubrol W
References
Page 2.11
CHAPTER 3: THE FLUID MOSAIC MEMBRANE
Table 3.1. Phase transition temperatures for the transition from ordered to disordered
hydrocarbon chains in diacyl phospholipids in hydrated multilayers
occur over a broader range of temperatures. As described by the fluid mosaic model, the
lipids in a biological membrane are typically in a fluid state at physiological temperatures.
In the ordered state, the fatty acid chains occupy less volume than in the fluid state. In the
case of phosphatidylcholine, this means that the hydrocarbon chains must tilt to achieve
adequate separation of the head groups.
Page 3.1
Bilayers composed of mixed lipids may exhibit phase separation. If the chemical
potentials of the individual lipids are higher in the mixed phase than in homogeneous
phases, then the homogeneous phases will separate out. This can be detected as a spatial
separation of different lipids or as domains having different characteristics (e.g., fluidity).
A striking example of this is the ripple phase exhibited by phosphatidylcholine bilayers. At
low temperatures, the bilayer forms a homogeneous ordered phase and, at high
temperatures, it forms a single fluid phase. At intermediate temperatures, however, the
bilayer exhibits a periodic pattern showing an undulating pattern or ripples on its surface.
The molecular basis for this is not yet clear, but some inferences can be made. In the
intermediate temperature range, phospholipids with tilted chains coexist with
phospholipids with extended chains. The differences in chain tilting and bilayer thickness
discourage intermixing of phospholipids in different phases and causes the two groups to
segregate (Marden et al., 1984).
Lipids in natural membranes are characteristically distributed asymmetrically in the
two halves of the bilayer. For example, human red cells are mostly PC on the outside and
mostly PE on the inside (Table 3.2). This asymmetry can be maintained because
phospholipids are very slow to redistribute (flip-flop) between the two sides of a bilayer.
The original cause of the asymmetry may lie in the biosynthetic history of the membrane,
but there also appear to be ATPases that invest cellular energy in the transport of
phospholipid head groups across a variety of natural membranes (Devaux, 1992).
Page 3.2
Measuring the electrical properties of membranes requires a planar membrane
separating two aqueous spaces large enough to accommodate electrodes. Planar bilayers
can be made by spreading lipid in solvent over an aperture (Mueller et al., 1963) or by
raising two monolayers past the aperture (Montal and Mueller, 1972). This produces
artificial membranes with much less total surface area than a liposome suspension, but it
does permit electrical recording of artificial membrane properties. In the solvent/aperture
method, the solvent in which the lipid is dissolved moves to the rim of the aperture and the
bilayer across the opening thins out to form a "black lipid membrane." Nevertheless, the
solvent can form lenses in the artificial membrane and there is always some question about
the influence of remaining solvent on the properties of the membrane. The monolayer
method corrects this. Even using great care, planar membranes formed by either method
are relatively unstable and their short lifetime is an experimental handicap.
To study membrane proteins, liposomes formed by dialysis have proven most
convenient. The proteins can be solubilized in the detergent and then conveniently
reconstituted. Monitoring effects of these proteins on electrical properties of the
membrane is not possible, however, because liposomes are too small to insert electrodes.
To overcome this problem a number of new approaches have been tried. First, liposomes
containing the reconstituted protein may be fused into a black lipid membrane (Miller and
Racker, 1976). Patch clamping technology has introduced a new approach. A patch
pipette can be raised through a lipid monolayer on the surface of a solution and then
lowered back down. A planar bilayer forms across the opening of the patch pipette and this
bilayer will contain proteins dispersed in the lipid monolayer (Tank et al., 1982; Suarez-
Isla et al., 1983).
Page 3.3
Section 3.3. Lipid Components
Fatty acids: Fatty acids have two characteristics that affect the physical properties
of the membrane: chain length and degree of unsaturation. Fatty acids may be identified
by a common name, by the standard nomenclature, or by the w nomenclature. According
to the standard nomenclature, fatty acids are represented as x:y, z1,z2 ... zn where x
represents the number of carbon atoms, y represents the number of double bonds, and z1,z2
... zn represent the carbon atoms preceding double bonds counting from the carboxy end.
According to the w nomenclature, fatty acids are represented as x:yωz' where x represents
the number of carbons, y represents the number of double bonds, and ωz' represents the
position of the first double bond counting from the ω carbon (methyl terminus). For
example, the structure of palmitoleic acid (16:1, 9-cis or 1ω7) is:
CH3-(CH2)4-CH2CH=CH(CH2)7COOH
Saturated Unsaturated
No. of Common Common Nomenclature
Carbons Name Name Standard ω
___________________________________________________________________
10 Capric Palmitoleic 16:1, 9-cis 1ω7
12 Lauric Oleic 18:1, 9-trans 1ω9
14 Myristic Vaccenic 18:1, 11-cis 1ω7
16 Palmitic Linoleic 18:2, 9-cis,12-cis 2ω6
18 Stearic α-Linolenic 18:3, 9-cis,12-cis,15-cis- 3ω3
20 Arachidic γ-Linolenic 18:3, 6-cis,9-cis,12-cis- 3ω6
22 Behenic Arachidonic 20:4, 5,8,11,14 (all cis) 4ω6
24 Lignoceric
___________________________________________________________________
O-
Headgroup-O-P-O- CH2 O
O CH-O-C-CH2...CH3
CH2-O-C-CH2...CH3
O
Page 3.4
The head groups - which differ in charge, polarity and reactivity - give the phospholipids
different characteristics:
Phosphat idylcholine (PC or lecit hin)
CH3 O-
CH3 O
NH3 + CH CH2 O P O
COO- O
HOCH CH O P O
HOHC CHOH O
O-
HO P O
Page 3.5
Cholesterol: Cholesterol is present in plasma membranes, lysosomes and storage
granules. The cholesterol content of the Golgi complex increases on moving from the cis
to the trans cisternae. Cholesterol is not present in bacterial, inner mitochondrial or
chloroplast thylakoid membranes.
CH3
21
20 22
12 18
CH3
24
17
11 23
13 25
CH3 C D 16 CH3
1 19 26 27
2 9 14 CH3
15
10 8
A B
7
HO 3 5
4 6
Cholesterol
Page 3.6
To predict which segments in a protein are membrane-spanning regions, it has
become common to use a hydropathy index. The most widely used is that described by
Kyte and Doolittle (1982). A variety of thermodynamic parameters could be used to assign
a hydropathy value to each amino acid. The validity of any particular choice may be
debated, but the only important consideration is that some consensus index of hydropathy
is established for each amino acid side chain. Kyte and Doolittle based their hydrophathy
index on the water-vapor transfer free energies of the side chains and on the interior-
exterior distribution of amino acid side chains. The hydropathy value of a span of amino
acids is then determined by summing the hydropathy indices for each amino acid in that
span. It is convenient to choose an odd number for the span length so the hydropathy index
can be associated with the amino acid in the middle of the span. For example, if a span of
7 is chosen, the hydropathy value at amino acid 40 is the sum of the hydropathy indices of
amino acids 37-43.
Page 3.7
or the protein will denature. The strategy used to solubilize membrane proteins is to add
detergent to the membrane. The detergent intercalates into the lipid bilayer forming a
mixed micelle with the phospholipids. When enough detergent has entered the membrane,
the structure changes from the bilayer favored by phospholipids to the oblate or spherical
micelles favored by the detergent. As this happens, the bilayer structure breaks down and
the proteins escape into soluble particles consisting of the protein along with detergent and
residual phospholipid. In principle, any detergent can be used to break down the lipid
bilayer and solubilize membrane proteins. In practice, however, strong detergents may also
enter into the protein itself and denature it. Dodecyl sulfate is a good example. It is useful
in gel electrophoresis because it disrupts the secondary and tertiary structure of a protein
causing it to migrate on the gel according to size alone. Dodecyl sulfate definitely
solubilizes membrane proteins, but it also denatures them destroying their structure and
activity.
Many membrane proteins (e.g., channels, transporters) have no assayable function
after they are solubilized and removed from the membrane. In order to study their
functions, they must be reconstituted into some sort of a membrane structure.
Reconstitution is typically a matter of simply reversing the solubilization process.
Phospholipids are reintroduced, detergent is removed, and the particle in which the protein
is situated changes from a micelle back into a bilayer. The objective here is to remove the
detergent but not the phospholipid. This can be accomplished by dialysis or gel filtration.
Because phospholipids have an extremely low critical micelle concentration, the rate at
which free phospholipids are removed is extremely slow. Obviously, the same will be true
for a detergent with a low cmc, making such a detergent (Triton X-100 is an example)
difficult to use in reconstitution. A detergent with a higher cmc can be removed relatively
rapidly, however, permitting reconstitution to occur successfully. The bile acids, cholic
acid and deoxycholic acid, have been particularly useful in solubilization and reconstitution
of membrane proteins. They have a high cmc, making it easy to remove them by dialysis.
Because they are ionic, their effectiveness as detergents depends on the ionic strength, and
the salt concentration can be manipulated to shift the balance between solubilization and
reconstitution. The bile acids also have a structure that is particularly suited to solubilizing
membrane proteins without denaturing them. These molecules have a generally planar
structure with a polar side and a nonpolar side. The nonpolar side protects the hydrophobic
protein surface while the other side faces the water. At the same time, this asymmetrical
polarity does not favor penetration by the detergent into the hydrophobic core of the
protein.
References
D. Deamer and A.D. Bangham (1976) Large volume liposomes by an ether vaporization
method, Biochim. Biophys. Acta 443, 629-634.
P.F. Devaux (1992) Protein involvement in transmembrane lipid asymmetry, Ann. Rev.
Biophys. Biomol. Struct. 21, 417-439.
C.H. Huang (1969) Studies on phosphatidylcholine vesicles. Formation and physical
characteristics, Biochemistry 8, 344-352.
J. Kyte and R.F. Doolittle (1982) A simple method for displaying the hydropathic character
of a protein, J. Mol. Biol. 157, 105-132.
Page 3.8
Fundamental Principles
of Membrane Biophysics
David Njus
© D. Njus, 2000
M. Marder, H.L. Frisch, J.S. Langer, and H.M. McConnell (1984) Theory of the
intermediate rippled phase of phospholipid bilayers, Proc. Natl. Acad. Sci. USA 81,
6559-6561.
C. Miller and E. Racker (1976) Ca2+-induced fusion of fragmented sarcoplasmic reticulum
with artificial planar bilayers, J. Memb. Biol. 30, 283-300.
M.H.W. Milsmann, R.A. Schwendener and H.G. Weder (1978) The preparation of large
single bilayer liposomes by a fast and controlled dialysis, Biochim. Biophys. Acta
512, 147-155.
M. Montal and P. Mueller (1972) Formation of bimolecular membranes from lipid
monolayers and a study of their electrical properties, Proc. Natl. Acad. Sci. USA 69,
3561-3566.
P. Mueller, D.O. Rudin, H.T. Tien, and W.C. Wescott (1963) Methods for the formation of
single bimolecular lipid membranes in aqueous solution, J. Phys. Chem 67, 534-
535.
J.R. Silvius (1992) Solubilization and functional reconstitution of biomembrane
components, Ann. Rev. Biophys. Biomol. Struct. 21, 323-348.
B.A. Suarez-Isla, K. Wan, J. Lindstrom, and M. Montal (1983) Single-channel recordings
from purified acetylcholine receptors reconstituted in bilayers formed at the tip of
patch pipets, Biochemistry 22, 2319-2323.
D.W. Tank, C. Miller, and W. Webb (1982) Isolated-patch recording from liposomes
containing functionally reconstituted chloride channels from Torpedo electroplax,
Proc. Natl. Acad. Sci. USA 79, 7749-7753.
G. von Heijne (1994) Membrane proteins: From sequence to structure, Ann. Rev. Biophys.
Biomol. Struct. 23, 167-192.
Page 3.9
Fundamental Principles
of Membrane Biophysics
David Njus
© D. Njus, 2000
CHAPTER 4: MEMBRANE ELECTROSTATICS
(4.1) F = (q1q2)/(4πεox2)
(4.2) W = - ∫ F dx
If a charge is located in the vicinity of many other charges, the force on it will be the sum
of the forces exerted by the other charges. In such a case, it is often more convenient to
define the electric field at a point x as the force exerted by all of the charges in the
neighborhood on a unit charge at that point. Thus, the electric field created by a point
charge q at a distance x is
The electrical potential ψ(x) is the work that must be done to move a unit charge from a
reference point to a point x.
x
(4.4) ψ(x) = - ∫ ref
E dx
Page 4.1
as in Figure 4.1A, it will create an electric field perpendicular to the plane. The electric
field will be proportional to the charge density on the plane (qs):
(4.5) E = qs/2εo
If we have two parallel planes of charge, one with a positive surface charge density
(+qs) and one with an equal density of negative charge (-qs), their electric fields will be
additive in the space between them and will cancel in the spaces on either side (Figure
4.1B). Between the planes, therefore, the total electric field will be qs/εo; outside of that
space, the total electric field will be zero. This arrangement is the common textbook
example, the parallel plate capacitor. Because there is an electric field between the two
plates of the parallel plate capacitor, there will be a difference in electrical potential
x x
(4.6) ∆ψ = - ∫0 E dx = - ∫0 (qs/εo) dx = - qsx/εo
This is the potential energy that must be expended to move a unit charge from plate 1 (at 0)
to plate 2 (at x).
A B
+q s +q s -q s
-q s +q s qs
E = E = E =0 E = E =0
2 ε 2 ε ε
ο ο ο
0 x
The preceding equations apply to charges arranged in a vacuum. In the real world,
charges will be separated by a medium composed of polarizable molecules. These
molecules will tend to align with the electric field and cancel it. The electric field will be
diminished in proportion to the polarizability of the medium, expressed as the dielectric
constant ε. Therefore, if the plates of a parallel plate capacitor are separated by a medium
of dielectric constant ε, the electric field between the plates will be
(4.7) E = qs/εεo
Page 4.2
Of course, the electrical potential difference between the plates will be correspondingly
smaller as well:
(4.8) ∆ψ = - qsx/εεo
Water, which has a dielectric constant of 80, is very polarizable. For that reason, charge
separation across water will tend to create a relatively small electric field and small
electrical potential differences. By contrast, hydrocarbons such as hexane (ε = 1.89) have
a very low dielectric constant. For that reason, charge separation across organic phases
(such as the interior of biological membranes) will create large electric fields. It is this low
dielectric constant that makes it possible to create significant electrical potential
differences across biological membranes.
Page 4.3
Outside Membrane Inside
potential
created by
capacitative
∆ψ
charge
dipole
potential
(outside)
0
surface
potential (outside) dipole membrane
potential potential
(inside)
surface
potential (inside)
When charge is moved from one side of a biological membrane to the other, we
assume that the net charge on one side is equal and opposite to the net charge on the other.
This is strictly required to maintain overall electroneutrality. These equal and opposite
charges separated by the low dielectric medium of the lipid membrane form an electrical
arrangement like the parallel plate capacitor. We will call the charge transferred across the
membrane the capacitative charge qc. The separation of this capacitative charge will create
an electric field in the membrane
(4.9) E = qc/Aεεo
(4.10) ∆ψ = qcx/Aεεo
The capacitance C is the ratio of the capacitative charge to the potential difference:
The capacitance, therefore, is proportional to the area of the membrane and inversely
proportional to the thickness. For a biological membrane, the capacitance is typically
Page 4.4
about 1 µF/cm2. This corresponds to a membrane 35 Å thick with a dielectric constant of
4.
The total membrane potential will include contributions from the capacitative
charge, from the surface charge on each side of the membrane, and from the surface dipole
on each side of the membrane (Figure 4.2). Because the contributions of surface charge
and surface dipole on one side of the membrane will tend to cancel the contributions of
those components on the other side, the capacitative charge is generally the primary
determinant of the total membrane potential.
qs q v (x)
ψ (∞) = 0
ψ (x)
ψ
ψo
0 x
Page 4.5
First, we imagine a plane of fixed charge with a surface charge density qs. Adjacent
to it is a medium with a space charge density qv(x) where x is the distance from the surface
(Figure 4.3). Overall electroneutrality requires that the total space charge be equal and
opposite to the sum of the fixed charge qs and the capacitative charge qc where the
capacitative charge is the net charge on the other side of the membrane. As we shall see,
the capacitative charge is usually small compared to the surface charge. For simplicity, we
shall simply include it as part of qs:
∞
(4.12) - ∫0 qv(x) dx = qs
The space charge at any point x is obtained by summing over all of the ionic species
present in the solution:
For each ion species i, zi is the valence and Ci is the concentration in moles/liter. F is the
Faraday constant and α is the constant 103 l/m3. Since the ion concentrations should
depend on the electrical potential according to the Boltzmann distribution:
where Cio is the concentration of ion species i far from the membrane (x=∞). The
Boltzmann distribution (eq. 4.14) together with equation 4.13 relates qv(x) to ψ(x).
Poisson's equation can be rationalized by considering the single plane of charge (Figure
4.1A). If the plane is assumed to have a thickness dx, the change in the electric field dE/dx
upon crossing the plane is qs/εεo. If equation 4.4 is differentiated twice, it is apparent that
Poisson's equation (eq. 4.16) can be integrated in two ways. First, using eq. 4.12,
∞ ∞
(4.18) qs = - ∫0 qv(x) dx = εεo ∫0 (d2ψ/dx2) dx
We define ψ(∞) = 0, so
Page 4.6
Second, using eq. 4.15 and 4.16,
To integrate equation 4.20, we multiply both sides by 2dψ/dx. We can then integrate this
equation to obtain
Equation 4.23 is the general form of the Gouy-Chapman equation relating the
surface charge to the surface potential. A more convenient form is obtained by assuming
that all of the ions in the aqueous phase are univalent. We will let Cio = Co for both anions
and cations. Then equation 4.23 simplifies to
It is apparent that the surface potential depends on the magnitude of the surface
charge (Figure 4.4) and also on the ionic strength of the aqueous medium (Figure 4.5).
Clearly, the counterions necessary to neutralize the fixed charge will have a much greater
effect on the ion concentration near the membrane surface if the ion concentration is low.
At low ionic strength, therefore, the concentration gradient will be greater and diffusion
forces will be stronger. This will drive the counterions farther from the surface leading to a
greater charge separation and a larger potential difference.
Another way of visualizing this is to simplify equation 4.24 by expanding the sinh
as a power series and dropping higher order terms (assume ψo is small). Equation 4.24
then reduces to
(4.25) qs = (8RTCoαεεo)1/2(Fψo/2RT)
The left-hand side of this equation is equal to the inverse of the distance x between plates
of a parallel plate capacitor (equation 4.8). A constant κ with units of m-1 is customarily
defined as
Page 4.7
(4.27) κ2 = (αF2/RTεεo) Σi Cio zi2
If all of the ions in the aqueous phase are univalent, then κ is equal to the right-hand side of
equation 4.26. Therefore,
(4.28) 1/x = κ
One may think of 1/κ as a measure of the thickness of the diffuse double layer. That is, the
same surface potential ψo would result if all of the diffusable charge were placed at a
distance 1/κ from the surface of the membrane.
In the foregoing analysis, we have incorporated the capacitative charge into the
surface charge. In actual practice, the capacitative charge is usually negligible compared to
the fixed surface charge and contributes little to the surface potential. To illustrate this, we
may note that the capacitative charge will rarely be greater than 10-7 coul/cm2 since that
charge will create a 100 mV membrane potential given the usual membrane capacitance of
1 µF/cm2. Typical surface charge densities are much larger than this (chromaffin granules
have a surface charge density of -1.38 x 10-6 coul/cm2).
Figure 4.4. The surface potential as a function of the surface charge density, according to
the Gouy-Chapman equation. The curve is for a univalent electrolyte at 10 mM
concentration. The dielectric constant has been taken as 80 and the temperature as 20°C.
Page 4.8
Figure 4.5. The decay of potential from a surface.
The surface charge density has been assumed to be 0.0158 coul/m2. The electrolyte is
univalent and the dielectric constant and temperature have been taken as 80 and 20°C
respectively.
Page 4.9
directions and tend to cancel each other. However, as we shall see, the dipole potential
may be a major factor in determining the ionic permeability of the lipid bilayer.
The dipolar character of phospholipids seems to arise from the ester linkage
between the fatty acid groups and the glycerol backbone. The head groups of the
phospholipids are certainly polar. However, they seem not to contribute appreciably to the
dipole potential. This is partly attributable to the fact that the head groups lie on the
aqueous surface of the membrane where the dielectric constant of the medium tends to
neutralize the dipoles. Moreover, the head groups seem to lie flat on the surface of the
membrane so the dipole moment in the direction perpendicular to the membrane surface is
small.
The dipole potential is
(4.29) ∆ψ = D/εεo
where D is the surface dipole density in coul/m. To estimate the potential magnitude of
surface dipole effects, let us assume that a typical phospholipid has a dipole moment of 1.5
Debyes (1.5 x 10-18 esu.cm or 5 x 10-30 coul.m) and occupies a membrane area of 60 Å2.
This yields a surface dipole density of 8.34 x 10-12 coul/m and implies a dipole potential of
(1000/ε) mV. If we assume that the dielectric constant of membrane in the region of the
dipole layer membrane is between 4 and 10, the surface dipole should create a potential
difference of 100-250 mV.
The electric field set up by the capacitative charge may cause the dipoles to orient in a
direction perpendicular to the membrane. Thus, the surface dipole may change in response
to a membrane potential. In this way, the surface dipole will affect the electric field near
the membrane surface and may be important in modulating the response of membrane-
bound enzymes to the membrane potential.
References
R.G. Ashcroft, H.G.L. Coster, and J.R. Smith (1981) The molecular organisation of
bimolecular lipid membranes. The dielectric structure of the
hydrophilic/hydrophobic interface, Biochim. Biophys. Acta 643, 191-204.
R. Aveyard and D.A. Haydon (1973) An Introduction to the Principles of Surface
Chemistry, Cambridge University Press, Cambridge.
R.F. Flewelling and W.L. Hubbell (1986) Hydrophobic ion interactions within membranes,
Biophys. J. 49, 531-540.
R.F. Flewelling and W.L. Hubbell (1986) The membrane dipole potential in a total
membrane potential model, Biophys. J. 49, 541-552.
D.A. Haydon and S.B. Hladky (1972) Ion transport across thin lipid membranes: A critical
discussion of mechanisms in selected systems, Quart. Rev. Biophys. 5, 187-282.
S. McLaughlin (1977) Electrostatic potentials at membrane-solution interfaces, Current
Topics in Membrane Transport 9, 71-144.
S. McLaughlin and H. Harary (1976) The hydrophobic adsorption of charged molecules to
bilayer membranes: A test of the applicability of the Stern equation, Biochemistry
15, 1941-1948.
Page 4.10
P.L. Yeagle (1979) Effect of transmembrane electrical potential and micelle geometry on
phospholipid head group conformation, Arch. Biochem. Biophys. 198, 501-505.
Page 4.11
Fundamental Principles
of Membrane Biophysics
David Njus
© D. Njus, 2000
CHAPTER 5: SPECIFIC AND NON-SPECIFIC BINDING
(5.1) L + M = LM
where L is the free ligand, M signifies empty binding sites on the membrane, and LM
represents ligand bound to the membrane. The equilibrium constant K is
(5.2) K = [L][M]/[LM]
Let us define Lm as the concentration of bound ligand ([LM]) and Lmax as the total number
of binding sites on the membrane ([M]+[LM]). Then the equilibrium constant becomes
(5.3) K = [L](Lmax-Lm)/Lm
Equation 5.3 may be solved to yield an expression for the amount of bound ligand
Lm
The amount of bound ligand Lm depends upon the binding constant K, the number of
binding sites Lmax, and the concentration of ligand [L] at the surface of the membrane
(Figure 5.1). As described in Section 5.2, if the ligand is charged, its concentration at the
surface of the membrane [L] will not necessarily equal its concentration in the bulk of the
aqueous phase but will be related to it by the surface potential. As described in Section
5.3, the binding constant K reflects the change in free energy occurring upon binding.
Equation 5.3 may also be rearranged to yield the Scatchard equation:
This form is particularly useful because a plot of Lm/[L] vs. Lm is linear and may be used to
define the parameters Lmax and K (Figure 5.2).
Page 5.1
Lmax
Lmax K
Lm
Lm
Lmax [L]
2
K Lmax
[L] Lm
Obviously, the known ligand concentration in the bulk of the aqueous phase [L]∞ may be
used in place of the ligand concentration at the membrane surface [L] if the ligand is
uncharged or if the surface potential ψo is negligible. Since the surface potential depends
upon both the ionic strength of the solution and upon the surface charge density, it can be
considered negligible if the ionic strength is high or if the surface charge density is low. If
the surface potential is greater than a few millivolts, however, [L] will be significantly
different from [L]∞. For example, if the surface potential is -18 mV and the ligand is a
univalent anion, [L] will be only 50% of [L]∞, and using [L]∞ in place of [L] will
significantly change the appearance of the binding curve (Figure 5.3) and the Scatchard
plot (Figure 5.4).
A special problem occurs when a charged ligand binds to the membrane to such an
extent that the bound ligand itself affects the membrane surface charge. This is generally
not a problem for ligands that bind to receptors since the receptor density (Lmax) is typically
insignificant compared to the surface charge density of the membrane. Ligands that adsorb
to the membrane, however, may have a significant effect on the surface charge density.
This situation, first considered by Stern, has been analyzed more recently by McLaughlin
and Harary (1976). If we assume that the surface charge density of the membrane is
initially zero, then [L] = [L]∞ for low values of Lm. As Lm increases, however, the surface
potential will increase and binding will deviate in the direction expected in the presence of
a surface potential. Thus, the Scatchard plot will appear to curve (Figure 5.4).
Page 5.2
Section 5.3. The Binding Constant
The binding constant K reflects the standard free energy change upon binding. This
can be seen by recognizing that the free energy change for the reaction in equation 1 is
(5.8) ∆G° = RT ln K
For adsorption, the free energy change represents the free energy change for the transfer of
the ligand from water into the membrane. For receptors, the free energy change carries
additional significance. In this case, ligand binding not only changes the location of the
ligand, but it changes the conformation of the receptor to which the ligand binds. Thus, the
free energy of binding includes not just an affinity between the binding site and the protein
but a change in the conformation of the protein. The conformational change is crucial, of
course, because it activates the receptor thereby transmitting the ligand-binding signal.
Let us assume that the affinity of the non-specific binding sites is very weak compared to
the affinity of the specific sites (Kns >> Ksp). To measure specific binding, one uses ligand
Page 5.3
concentrations that range about Ksp, so [L] will be very small relative to the non-specific
binding constant [L] << Kns). Equation 5.9 then reduces to
so non-specific binding will be directly proportional to the ligand concentration over this
range. This non-specific binding can be measured by adding a high concentration of ligand
(Kns >> [L] >> Ksp). Under these conditions, equation 5.9 reduces to
Because Lmaxsp << Lmaxns, the measured binding Lm will all be non-specific. This gives the
slope of the non-specific binding line (Lmaxns / Kns). Then specific binding can be
calculated by subtracting non-specific binding from total binding as in Figure 5.5.
References
Page 5.4
Fundamental Principles
of Membrane Biophysics
David Njus
© D. Njus, 2000
CHAPTER 6: PERMEABILITY AND CONDUCTANCE
(6.1) µ = µ° + RT ln C + zFψ
At equilibrium, a given ion or molecule must have the same electrochemical potential on
both sides of the membrane. If we use the subscript i to denote one side of the membrane
(inside) and the subscript o to denote the other side (outside), then
This is the famous Nernst equation, which gives the equilibrium concentration gradient of
an electrolyte in the presence of a membrane potential. If the molecule is a non-electrolyte
(z=0) or if the membrane potential is zero, then equation 6.3 simplifies to
(6.4) Ci = C o
Page 6.1
having this activation energy is given by the Boltzmann distribution. Let us imagine that
the activation energy for a particular ion or molecule crossing the membrane is Ea (Figure
6.1). Then the rate of influx (Joi) is
Equation 6.7 reduces to the Nernst equation (equation 6.3), so the statistical mechanics
approach predicts the same equilibrium as does the thermodynamics approach.
Ea
ψ zF
o
ψ zF
i
Page 6.2
(6.9) [A-]i/[A-]o = [H+]o/[H+]i
To understand the logic behind this, let us consider the case of a weak base (Figure 6.2).
The uncharged species (B) will reach the equilibrium expected of nonelectrolytes (Bo = Bi).
On either side of the membrane, this unprotonated species will be in equilibrium with the
protonated form:
Since [B]o = [B]i, equation 6.10 reduces to equation 6.8. A similar analysis may be applied
to weak acids to establish equation 6.9.
BH + BH +
H++ B B + H +
N is Avogadro's number and f is the frictional coefficient. The total rate of flow J is the
velocity multiplied by the concentration C:
(6.12) J = vC = -(C/Nf)(dµ/dx)
If we define RT/Nf as the diffusion coefficient D, equation 6.13 reduces to Fick's Law of
diffusion.
Page 6.3
Membrane permeation involves diffusion across the membrane. To analyze this,
we must integrate Fick's Law (equation 6.13) from one side of the membrane to the other
(Figure 3).
d d
(6.14) ∫0 J dx = - ∫0 (RT/Nf)(dC/dx)dx
Ci
Cmi
Co
C mo
0 d
This integration, which allows us to determine how the flow depends on the overall
concentration gradient (Ci - Co) across the membrane, is relatively simple. As we shall see
(Section 7.1), the corresponding integration for the case of non-electrolytes is much more
difficult.
To integrate equation 6.14, we assume that the flow of non-electrolyte across the
membrane reaches a steady state. This means that the flow J must be the same at all points
within the membrane so that the concentration profile across the membrane does not
change with time. Steady-state is a natural assumption because it is a stable condition.
Suppose that J varied within the membrane so that the flow into a particular region was
greater than the flow out of that region. The concentration of non-electrolyte in that region
would then rise. This, in turn, would cause the flow out of the region to increase and the
flow into the region to decrease. Thus, the concentration would spontaneously change to
maintain equality between the flows into and out of the region. This assures steady state.
We may then integrate equation 6.14 assuming that J is independent of x (constant).
(6.15) Jd = -(RT/Nf)(Cmi-Cmo)
Cmi is the concentration in the membrane at the inner surface and Cmo is the concentration
in the membrane at the outer surface.
We imagine that, at each surface of the membrane, molecules in the aqueous phase
are in equilibrium with molecules in the membrane phase. Therefore, the chemical
potential in the water phase (µw) must equal the chemical potential in the membrane (µm):
Page 6.4
The concentration at the surface of the membrane (Cm) is then
An expression for J is obtained by substituting equation 6.17 into equation 6.15 and
rearranging:
The flux is proportional to the concentration difference across the membrane (Ci - Co). It is
also proportional to the permeability coefficient P defined as
The permeability coefficient includes the term RT/Nf, which is the diffusion coefficient of
the molecule in the lipid phase of the membrane. This term will depend on the size of the
molecule. The term exp[(µw°-µm°)/RT] describes the partitioning of the molecule between
water and the membrane and will depend on the lipid solubility of the molecule. Since
Cm/Cw is the membrane:water partition coefficient (Kp), equation 6.17 implies that
A comparison of equations 6.19 and 6.20 shows that P and Kp should be linearly related.
Walter and Gutknecht (1984) tested this prediction in a study of the permeability of lipid
bilayers to a series of carboxylic acids. After correcting for unstirred layer effects and
assuming that only the protonated form of the carboxylic acid permeates, they found that
the permeability coefficient is related to the hexadecane:water partition coefficient (Kp') as
follows:
The observed slope of 0.90 is close to the predicted slope of 1.0. Moreover, the free energy
change for the transfer of the carboxylic acid from water into the membrane (µm°-µw°) can
be determined from either the permeability coefficient P or the partition coefficient Kp.
The incremental change in the free energy per methylene group for the series of carboxylic
acids (acetic, propionic, butyric, hexanoic) is -898 ± 159 cal/mole determined from the
partition coefficient and -764 ± 54 cal/mole determined from the permeability coefficient.
These numbers agree very well with the energies described in the discussion on micelle
formation (Section 2.4).
Page 6.5
thick). The unstirred layer effect will generally be most prominent for relatively nonpolar
compounds. For these compounds, the permeability coefficient will be large and diffusion
across the membrane itself will be relatively fast. Diffusion across the aqueous layers,
therefore, may be partially rate-limiting. For compounds that are quite water soluble,
permeation across the membrane will be slow, and diffusion across the unstirred layers will
have relatively less effect.
To consider the effect of unstirred layers, imagine that a compound has a membrane
permeability coefficient P and an aqueous diffusion constant D. Let the unstirred layers
have thicknesses, di and do (Figure 6.4), let the bulk concentrations of the compound be Ci
(inside) and Co (outside) and let the concentrations at the surface of the membrane be Cmi
and Cmo. The flow through the membrane then is
At steady-state, the flows must all be the same (Jm = Ji = Jo = J). Therefore, equations 6.22,
6.23 and 6.24 can be transformed to
Unstirred Unstirred
Layer Membrane Layer
do d
i
Ci
C mi
C mo
Co
Page 6.6
Figure 6.4. Permeation and diffusion through unstirred layers
Therefore, the effect of unstirred layers is to decrease the permeability so the apparent
permeability coefficient (Papp) is smaller than P:
References
Page 6.7
Fundamental Principles
of Membrane Biophysics
David Njus
© D. Njus, 2000
CHAPTER 7: PERMEABILITY AND CONDUCTANCE OF ELECTROLYTES
dψ
(7.1) J = - ( ) C
Nf
d
dx [ µ o + RT ln C + zF ψ ] = - RT
Nf [ dC
dx + ( zFC
RT ) ]
dx
As before, we must integrate this from one side of the membrane to the other. In
this case, however, we have two parameters, C and ψ, that will vary as we cross the
membrane. We may use the steady-state assumption to define one of these parameters in
terms of the other, but we will need another equation to define both. We will return to this
problem later.
First, note that
zFC d ψ ψ/RT)
(zFψ /RT)
] [ dx ] e(zF
dC
[Ce
d
(7.2) = +
dx RT dx
A comparison of equations 7.1 and 7.2 reveals that exp (zFψ/RT) may be used as an
integrating factor so that
If we make the steady-state assumption (J is constant across the membrane), then we may
partially integrate equation 7.3:
zFψ/RT zFψmi/RT zFψmo/RT
d
(7.4) J ∫0 e dx = - (RT/Nf) [Cmi e - Cmo e ]
Cmi and Cmo are the concentrations in the membrane at d and 0 respectively (Figure 7.1).
They are related to the concentrations Ci and Co in the bulk aqueous phases by the
membrane:water partition coefficient (Kp) and by the surface potential:
Therefore,
ψ ψ
(7.7) J ∫
d zF ψ /RT RTK p ( C e zF i /RT - C e zF o /RT )
dx = -
0 e Nf i o
If we define the electrical potential as zero on the outside (ψo = 0), then
Page 7.1
zF ψ m /RT
( )
d
(7.8) J = RTKp
Nfd d
∫ exp (zFψ/RT) dx
[ Co - C i e ]
0
where ψm is the membrane potential. We may note that RTKp/Nfd is the permeability
coefficient P (equations 6.19 and 6.20). Therefore,
where Q is defined as
d
(7.10) Q = d/∫0 exp (zFψ/RT) dx
C mo ψi
Co ψ mi
ψ
o
ψ
mo
0 d
Page 7.2
zF ψ m/RT
(7.12) Q =
exp (zF ψ /RT) - 1
m
Introducing equation 7.12 into equation 7.9 yields a final expression for the flow J:
zF ψm /RT zF ψ m /RT
(7.13) J = P ( exp (zF ψm /RT) - 1 ) (Co - C i e )
Equations 7.9 and 7.13 provide us with alternative expressions for determining the
flow of electrolytes across biological membranes with the latter equation including the
assumption of a constant field. These expressions allow us to calculate the flow of
electrolyte driven across the membrane by a membrane potential, by a concentration
gradient, or by a combination of these two forces. In Figure 7.2, the membrane potential
and the concentration gradient are used as the axes of a two-dimensional graph. Equations
7.9 and 7.13, therefore, allow us to calculate the flow at any point on this graph. Let us
examine some special cases. First, note that both equations reduce to the Nernst equation
if the flow J is zero:
Finally, if there is no concentration gradient (Ci/Co = 1), equation 7.13 reduces to:
(7.16) J = - PCzFψm/RT
Since I = -JFz, equation 7.16 is equivalent to Ohm's Law (I = g ψm) where the conductance
g is PCz2F2/RT.
Page 7.3
zψ Ohm's Law zψ Ohm's Law
0
C i /C o ln C i /C o
Nernst
Equation
Nernst
Equation
0 1 0
The net flow J will be the difference between these two fluxes:
Page 7.4
Energy Outside Membrane Inside
Em
Eo
Ei
Following our usual convention, we will define the electrical potential as zero on the
outside. Then Eo will simply be the standard free energy of the electrolyte in water (Eo =
µw°) and Ei will differ from this by the membrane potential (Ei = µw° + zFψm). Upon
introducing these values for the energies, equation 7.19 reduces to
If we let PQ = exp [-(Em- µw°)/RT] x constant, then equation 7.20 is the same as the flux
equation derived using the electrochemical potential approach (equation 7.9).
Page 7.5
dq
x a
(7.21) W = q2/8πεεoa
This equation can be derived as follows. Outside of a conducting sphere, the electric field
created by the sphere is the same as the electric field created by a point charge (of equal
charge) located at the center of the sphere. Therefore, the force between a sphere of charge
q' and a charge dq' is given by Coulomb's Law:
(7.22) F = q'dq'/4πεεox2
The work required to move the charge dq onto the sphere from an infinite distance away is
a a
(7.23) dW = - ∫∞ F dx = - ∫∞ (q' dq'/4πεεox2) dx
= q' dq'/4πεεoa
The work required to place the entire charge q on the sphere is then
q
(7.24) W = ∫ δW = ∫0 q' dq'/4πεεoa = q2/8πεεoa
The change in the charging energy upon moving the ion from water into a
hydrocarbon phase is
The membrane is not an infinite hydrocarbon phase, but is a thin layer of hydrocarbon with
water on both sides. Therefore, the change in charging energy upon moving the ion from
water into a membrane is somewhat smaller than the change shown in equation 7.25. The
work done in moving a charge q a distance x into a membrane of thickness d has been
approximated by Flewelling and Hubbell (1986):
Page 7.6
( )( )[ ( )( ) ]
2 1 a - 1.2 x 2
q 1 - 2x a
(7.26) W = 8aπε o ε hc d d
The fluxes Jj are defined by equation 7.13. If we impose the simplifying assumption that
all of the ions are univalent, then
Dividing by Fψm/RT and rearranging the exponentials in the anion term yields
Σ cations P jC oj + Σ anions P jC ij
(7.31) exp (Fψ /RT) =
m
Σ cations P jC ij + Σ anions P C oj
j
Therefore, the membrane potential is defined by the ion concentrations and permeabilities
as follows:
Σ cations P jC oj + Σ anions P jC ij
(7.32) ψ = (RT/F) ln
m Σ cations P jC ij + Σ anions P C oj
j
Page 7.7
References
R.F. Flewelling and W.L. Hubbell (1986) Hydrophobic ion interactions within membranes,
Biophys. J. 49, 531-540.
R.F. Flewelling and W.L. Hubbell (1986) The membrane dipole potential in a total
membrane potential model, Biophys. J. 49, 541-552.
A.L. Hodgkin and A.F. Huxley (1952) A quantitative description of membrane current and
its application to conduction and excitation in nerve, J. Physiol. 117, 500-544.
A.R. Koch (1970) Transport equations and criteria for active transport, Am. Zoologist 10,
331-346.
R.I. Macey (1978) Mathematical models of membrane transport processes, in Membrane
Physiology (T.E. Andreoli, J.F. Hoffman, and D.D. Fanestil, eds.), Plenum, New
York, pp. 125-146.
A. Parsegian (1969) Energy of an ion crossing a low dielectric membrane: Solutions to
four relevant electrostatic problems, Nature 221, 844-846.
Page 7.8
Fundamental Principles
of Membrane Biophysics
David Njus
© D. Njus, 2000
CHAPTER 8: CHANNELS AND EXCITABLE MEMBRANES
HCO-L-Val-Gly-L-Ala-D-Leu-L-Ala-D-Val-L-Val-D-Val-L-Trp-
D-Leu-L-Trp-D-Leu-L-Trp-D-Leu-L-Trp-NHCH2CH2OH
The conductance through gramicidin channels varies as the square of the gramicidin
concentration indicating that the compound functions as a dimer. Indeed, two peptides can
be linked at the formyl groups on their amino terminal ends, and the coupled structure will
function as a channel. The channel formed by gramicidin is not very selective and has a
unitary (single channel) conductance of 5 pS. It is thought that gramicidin forms a helix
with the hydrophobic side chains on the outside and the carbonyl oxygens oriented to the
inside. This forms a channel 2 Å in diameter.
Amphotericin B forms a larger channel and increases the permeability of lipid
bilayers to water and small electrolytes as well as ions. The conductance depends on the
4th-12th power of the concentration suggesting that a number of molecules are required to
form the channel. Because amphotericin B is an elongated molecule with a hydrophobic
side and a hydrophilic side, it is thought to line the sides of the channel like the staves on a
barrel. Amphotericin B requires a sterol for activity, and thus makes channels in
membranes that contain cholesterol.
Page 8.1
muscle also contains a β1 subunit (38 kDa), while the sodium channel in mammalian brain
contains both a β1 (36 kDa) and a β2 subunit (33 kDa) along with the α subunit.
Voltage-gated potassium channels include the delayed rectifier (DR) channel,
which functions in actions potentials in excitable membranes, and the CaK channel, which
is activated by Ca2+ as well as by depolarization. Both are blocked by barium. The CaK
channel has a very high unitary conductance and is specifically blocked by charybdotoxin.
Calcium channels have been classified into a variety of types based on functional
characteristics and pharmacology. The well characterized L-type channel is a high-
threshold, slow inactivating channel, which is blocked by dihydropyridines. Low-
threshold, fast inactivating Ca2+ channels are classed as T-type. Two other high-threshold
channels (N and P) are distinguished by their sensitivity to peptide toxins. N channels are
blocked by ω-conotoxin GVIA while P channels are blocked by ω-agatoxin IVA.
Structurally, these channels are thought to be similar. The L-type Ca2+ channel from
skeletal muscle has five subunits: α1 (170 kDa), α2δ (175 kDa), β (52 kDa) and γ (32
kDa). The α1 subunit contains binding sites for Ca2+ channel antagonists and is thought to
be the subunit forming the functional Ca2+ channel. The α2δ piece consists of a large α2
subunit linked to the δ subunit by disulfide bonds.
Page 8.2
"foot" structure. The remaining 20% on the carboxyl end includes 4 to 10 transmembrane
segments and presumably creates the ion channel structure. Ryanodine, a plant product,
opens this channel. In vivo, however, the channel is gated by cytoplasmic Ca2+. Thus,
when L-channels in the T-tubule membranes open and allow Ca2+ to enter the muscle cell,
the ryanodine receptor channel in the sarcoplasmic reticulum membrane responds by
releasing more Ca2+ from the SR. Thus, the ryanodine receptor mediates Ca2+-induced
Ca2+ release.
A related protein, the inositol-1,4,5-trisphosphate receptor releases Ca2+ from the
endoplasmic reticulum in response to the intracellular messenger inositol-1,4,5-
trisphosphate (IP3). It has a molecular mass of 260 kDa and, like the ryanodine receptor,
consists of a large amino-terminal foot and a smaller carboxyl portion containing 8-10
transmembrane segments. Both proteins appear to associate into homotetramers.
zF ψm /RT zF ψm /RT
(8.1) I = -FzP ( exp (zFψm /RT) - 1 )( Co - C i e )
The current carried by ion channels is commonly expressed using a variation on Ohm's
Law:
Page 8.3
Ii is the current carried by ion i, gi is the conductance of the membrane to that ion, ψm is the
membrane potential and ψi is the equilibrium potential for that ion as defined by the Nernst
equation (equation 6.3). Adjusting the membrane potential by subtracting ψi insures that
there will be no current when the membrane potential equals the ion’s equilibrium
potential. Equation 8.2 predicts that a plot of current (Ii) against voltage (ψm), also known
as an IV curve, will be linear. The slope is the conductance gi and the X-intercept is the
equilibrium potential ψi.
If a channel is perfectly selective for a particular ion, that channel will exhibit an IV
curve as defined by equation 8.2. Often, however, a channel is not absolutely selective and
will pass different kinds of ions with different conductances. In this case, the current
through the channel must be summed over the different ions:
For a non-selective channel, Ohm's Law still holds, but the conductance is the sum of the
conductances for all of the ions, and the X-intercept is a weighted average of the
equilibrium potentials. The X-intercept is called the reversal potential because the current
through the channel reverses direction as the membrane potential crosses this value. If the
channel is highly selective for a particular ion, the reversal potential will be close to the
equilibrium potential for that ion. Thus, the reversal potential is an indicator of a channel's
ion selectivity. Note that the reversal potential of a channel depends on the channel's
selectivity and also on the equilibrium potentials (concentration gradients) of the ions it
conducts.
The functioning of individual channels has been illuminated in recent years by the
development of the patch clamp technique. This technique, which permits the observation
of currents through single channels, has revealed the opening and closing times for
different channel types. It has also enabled measurement of unitary conductances. The
unitary conductance is important because it reflects the restriction imposed by the channel's
selectivity filter. For example, the sodium channel, which has a relatively low unitary
conductance, probably has a relatively long narrow tunnel. It is estimated that this is a pore
3 Å x 5 Å in cross section and 10-12 Å in length. The high conductance K channel, by
contrast, probably has a relatively short narrow tunnel. In both cases, it is imagined that
the channel has large vestibules on one or both sides of the selectivity filter. This permits
free (and rapid) diffusion through most of the membrane and limits conductance only to the
extent necessary to maintain selectivity.
Page 8.4
TABLE 8.2. The Eisenman Selectivity Series for Monovalent Cations
To cross through a channel, an ion must shed its water of hydration and pass a selectivity
site in the channel. This implies that the ion must interact more favorably with the
selectivity site than with its water of hydration. At low field strength, the selectivity of the
channel is dominated by the dehydration energy of the ion. Ions that are easily dehydrated
pass through the channel more readily than do ions that interact strongly with water. At
low field strength, therefore, larger ions are favored over smaller ions and the order is in
the direction of decreasing atomic weight. At high field strength, the selectivity of the
channel is dominated by the attraction of the ion for the selectivity site. Those ions that
bind well will be favored over those that bind poorly. At high field strength, therefore,
Intermediate-field-strength site
-∆G
High-field-strength site Hydration energy
Low-field-strength site
Page 8.5
smaller ions are favored over larger ions and the order is in the direction of increasing
atomic weight. The sodium channel has selectivity characteristics typical of a high field
strength. This high field strength is attributed to one or more carboxylic acid groups that
may line the tunnel and interact with the passing cations.
Type Selectivity
ACh Receptor NH4+ > Cs+ > Rb+ > Na+
Na+ Na+,Li+ > K+ > Rb+ > Cs+
K+ (DR) Tl+ > K+ > Rb+ > NH4+ > Na+ > Li+ >> Cs+
K+ (Ca2+) Tl+ > K+ > Rb+ > NH4+ > Na+, Li+, Cs+
Ca2+ (L) Ca2+ > Sr2+ > Ba2+ > Li+ > Na+ > K+ > Cs+ > Mg2+
Ca2+ (T) Ca2+ ≈ Ba2+
Page 8.6
Equation 8.4 includes currents of K+ and Na+ as well as a small residual current (mostly Cl-
), which is commonly known as the “leakage current.” Equation 8.4 may be expanded
knowing that the membrane potential is related to the capacitative charge by the
capacitance (Equation 4.11), and the currents are given by Ohm’s Law (Equation 8.2):
Equation 8.5 makes it clear that the change in the membrane potential ψm depends on the
conductances of the sodium and potassium channels. The sodium and potassium channels
in turn are gated by voltage, so their conductances change depending on the membrane
potential. To separate the interdependence of channel conductance and membrane
potential, Hodgkin and Huxley used the voltage-clamp technique, in which the membrane
potential is fixed or clamped so that Na+ and K+ currents can be recorded at that constant
membrane potential. They found that Na+ and K+ channels remain closed at the resting
membrane potential but their probability of opening increases when the membrane
potential is raised. When the membrane is depolarized, Na+ channels open quickly and
then inactivate or close. K+ channels open more slowly and remain open. This means that
depolarizing a membrane will cause Na+ channels to open first. The inward Na+ current
will drive the membrane potential in the positive direction toward the Na+ equilibrium
potential. This accelerates the opening of Na+ channels ensuring a strong depolarization.
After a brief time, however, the Na+ channels inactivate and the Na+ current stops.
Concurrently, K+ channels open, and the outward K+ current drives the membrane potential
back down toward the K+ equilibrium potential. This brings the membrane potential back
to the resting value and also causes the K+ channels to close terminating the action
potential.
From data gathered in their voltage clamp experiments, Hodgkin and Huxley found
empirical relations that express the changes in conductances as functions of the membrane
potential. These functions are quite complicated and for our purposes need only be
represented as f1(gK, ψm) and f2(gNa, ψm).
Knowing how the membrane potential depends on conductances (equation 8.5) and
how the conductances depend on membrane potential (equations 8.6 and 8.7), it is possible
to reconstruct the action potential. A membrane action potential is an action potential in
which the membrane potential changes along the whole length of the nerve fiber at the
same time. Thus, the entire nerve fiber depolarizes and repolarizes simultaneously. A
membrane action potential can be simulated by numerically integrating equations 8.5, 8.6
and 8.7. Assume that the membrane potential begins at a value somewhat above the
resting potential. Given this membrane potential, calculate the change in gNa, gK and ψm
expected to occur in a brief interval of time (say 10 µsec). After changing the values of
gNa, gK and ψm accordingly, calculate the changes expected in the next brief time interval
by repeating the process. If the initial membrane potential was set to a value above
Page 8.7
threshold, then the membrane potential will rise up in an action potential and then return to
rest. If the initial membrane potential was set to a value below threshold, then the
membrane potential will simply drift back down to its resting value.
The membrane action potential is easy to calculate, but normally an action potential
does not occur along an entire nerve fiber simultaneously. Rather, it travels from one end
to the other. To analyze this propagating action potential, recognize that the action
potential travels because charge diffuses down the nerve fiber, depolarizing the membrane
ahead of the action potential, and causing the action potential to move forward along the
fiber. The current along the length of the nerve fiber is given by Ohm’s Law:
Recognize that dx/dt is the conduction velocity of the action potential (θ). Then,
(8.11) Imem = (1/ πDrθ2) (d2ψm/dt2) = C (dψm/dt) + gK (ψm - ψK) + gNa (ψm - ψNa +
gl(ψm - ψl)
Equation 8.11 may be numerically integrated as for the membrane action potential if the
correct value for the conduction velocity θ is chosen.
References
Page 8.8
H.A. Lester (1992) The permeation pathway of neurotransmitter-gated ion channels, Ann.
Rev. Biophys. Biomol. Struct. 21, 267-292.
P.S. McPherson and K.P. Campbell (1993) The ryanodine receptor/Ca2+ release channel, J.
Biol. Chem. 268, 13765-13768.
R.J. Miller (1992) Voltage-sensitive Ca2+ channels, J. Biol. Chem. 267, 1403-1406.
M. Mishina, T. Kurosaki, T. Tobimatsu, Y. Morimoto, M. Noda, T. Yamamoto, M. Terao,
J. Lindstrom, T. Takahashi, M. Kuno and S. Numa (1984) Expression of functional
acetylcholine receptor from cloned cDNA, Nature 307, 604-608.
M. Noda, T. Ikeda, T. Kayano, H. Suzuki, H. Takeshima, M. Kurasaki, H. Takahashi, and
S. Numa (1986) Existence of distinct sodium channel messenger RNAs in rat brain,
Nature 320, 188-192.
M. Noda, S. Shimizu, T. Tanabe, T. Takai, T. Kayano, T. Ikeda, H. Takahashi, H.
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Hirose, S. Inayama, H. Hayashid, T. Miyata and S. Numa (1984) Primary structure
of Electrophorus electricus sodium channel deduced from cDNA sequence, Nature
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M. Noda, H. Takahashi, T. Tanabe, M. Toyosato, S. Kikyotani, Y. Furutani, T. Hirose, H.
Takashima, S. Inayama, T. Miyata and S. Numa, (1983) Structural homology of
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B.M. Olivera, G. Miljanich, J. Ramachandran, and M.E. Adams (1994) Calcium channel
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R.W. Tsien, P. Hess, E.W. McCleskey and R.L. Rosenberg (1987) Calcium channels:
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G. Yellen (1987) Permeation in potassium channels: Implications for channel structure,
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C.D. Ferris, and S.H. Snyder (1992) Inositol 1,4,5-trisphosphate activated calcium
channels, Ann. Rev. Physiol. 54,
Page 8.9
Fundamental Principles
of Membrane Biophysics
David Njus
© D. Njus, 2000
CHAPTER 9: ACTIVE TRANSPORT
Page 9.1
These ATPases transport H+ by a rotary mechanism and have a two-part structure including
a membrane stalk and a large head. They differ from the plasma membrane ATPases in
being insensitive to vanadate and in lacking a stable phosphorylated intermediate. The
subunit composition of the F, V and A ATPases is also much more complex than that of
the P-type ATPases. They are characteristically sensitive to N,N'-
dicyclohexylcarbodiimide (DCCD), which reacts with carboxyl groups and typically
inhibits proton-translocating enzymes.
The F or F1/FO class of H+-ATPases are found in mitochondria, chloroplasts and
bacterial cells and are, of course, responsible for ATP synthesis rather than ATP
hydrolysis. Nevertheless, they should be considered here because they are functionally
similar to the V and A type ATPases, and they can catalyze active transport of H+ when the
free energy of the reaction favors that direction. These enzymes have a complex subunit
composition including a hydrophilic F1 component which carries the catalytic site and a
hydrophobic FO component which is thought to be the proton channel. The F1 component
includes five kinds of subunits (α, β, γ, δ, ε) in a stoichiometry of 3:3:1:1:1. The
nucleotide binding sites lie between the α and β subunits. At any given time, these sites
are in the tight, loose or open state depending on the position of the γ subunit. The FO
component includes three kinds of subunits (a, b, c) in a stoichiometry of 1:2:~12. DCCD
inhibits the F1FO ATPases by reacting with a glutamate residue in the c subunit. The c
subunits are relatively small (2 transmembrane segments) and the cluster of c subunits
together with the γ subunit form an axle around which the α3β3 head rotates. In the ATP
hydrolysis mode, the F1FO ATPase is thought to function as follows: ATP hydrolysis
drives the rotation of the α3β3 head group relative to the γ subunit and the c cluster attached
at the base. This rotation causes the three nucleotide binding sites to step alternately
through the loose, tight and open conformations. In the process, ATP is bound, hydrolyzed
and the products released. Concomitantly, 3 H+ are pumped through the FO component for
each ATP hydrolyzed.
The V or vacuolar ATPases are similar in structure to the F ATPases, although their
normal function is H+ transport driven by ATP hydrolysis rather than ATP synthesis driven
by H+ movement. The V ATPases differ from the F ATPases in that they are inhibited by
the antibiotic bafilomycin A1 and by N-ethylmaleimide.
ATPases couple a chemical reaction to vectorial movement of an ion. For this to
work, three criteria must be met. 1) The energetics must be such that the chemical reaction
releases more free energy than is required for ion translocation. 2) The chemical reaction
and ion translocation must be obligatorily coupled processes. One cannot be allowed to
occur in the absence of the other. 3) The enzyme must change its affinity for the ions
during the translocation cycle.
First, let us consider the energetics. For ion transport, the change in the
electrochemical potential of the transported ion is:
Page 9.2
The total free energy change required for transport is the sum of the electrochemical
potential differences (∆µj) for each of the transported ions multiplied by the stoichiometry
nj for that ion:
If we define nj as positive for ions transported from outside to inside and negative for ions
transported from inside to outside, then
This convention simplifies the bookkeeping of directionality and allows us to use the
membrane potential (ψin- ψout) without worrying about the sign. Now, for the chemical
reaction, the free energy change is
where ∆G°ATP = -7.3 kcal/mol. Of course, ion transport will proceed as long as
Equation 9.5 emphasizes that the ATPases catalyze coupled reactions. It would not do for
either ATP hydrolysis or ion movement to occur independently. If ATP hydrolysis
occurred independently of ion movement, then the free energy released by ATP hydrolysis
would be wasted. If ion movement were allowed to proceed in the absence of ATP
hydrolysis, then the ions would move in the wrong direction: from higher concentration to
lower concentration.
To catalyze ion movement against a concentration gradient, the enzyme must take
the ion from a solution in which the ion is present at a lower concentration and release the
ion into a solution in which the ion is present at a higher concentration. Thus, in addition
to moving the ion, the enzyme must change its affinity for the ion in order to function
efficiently. The ATPase must have a high affinity for the ion on the low-concentration side
and a low affinity for the ion on the high-concentration side. Part of the energy released by
ATP hydrolysis, therefore, must go into changing the enzyme's affinity for the ion.
The free energy change associated with this reaction may be expressed in the usual way:
Page 9.3
[Donor]ox[Acceptor]red
(9.6) ∆Gredox = ∆G° + RT ln ( [Donor] red [Acceptor ]ox
)
Rather than tabulate ∆G° values for all combinations of electron carriers, however, electron
affinities are catalogued by considering each half- reaction separately:
Donorred → Donorox + n e-
Acceptorox + n e- → Acceptorred
where n is the number of electrons transferred in the reaction and E° is the midpoint or
standard reduction potential for the electron donor. A similar equation defines the
reduction potential of the electron acceptor. The reduction potentials are related to the free
energy change as
Page 9.4
Section 9.4. Bacteriorhodopsin
Bacteriorhodopsin is a light-driven proton pump. It is found in Halobacterium
halobium, a bacterium found growing in hot salt springs. In the presence of oxygen,
Halobacterium uses respiration as a source of metabolic energy. Under anaerobic
conditions, however, Halobacterium uses light energy to produce ATP directly.
Halobacterium is not photosynthetic since it does not split H2O (produce O2) or exhibit
light-driven electron flow. Instead, it produces a "purple membrane" which is essentially
pure bacteriorhodopsin: 25% lipid and 75% bacteriorhodopsin. Bacteriorhodopsin pumps
H+ out of the cell in the presence of light. This sets up an H+ gradient, just as respiration
would, and the proton gradient can be used to make ATP in the usual chemiosmotic way.
Bacteriorhodopsin is a particularly well characterized ion pump, because it can be
obtained in large quantity and essentially pure. Moreover, it is a rather simple protein
having a molecular weight of 26,000. The amino acid sequence has been determined
(Ovchinnikov et al., 1979) and its three-dimensional structure elucidated (Henderson and
Unwin, 1975). Bacteriorhodopsin consists of seven membrane-spanning domains. A
cofactor, retinal, is responsible for light absorption. Retinal is covalently bound to the ε
amino of a lysine residue via a Schiff's base linkage.
The energy of a quantum of light depends on the wavelength (λ):
where h is Planck's constant and c is the speed of light. For an einstein (mole) of quanta,
the energy is
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J.P. Abrahams, A.G.W. Leslie, R. Lutter, and J.E. Walker (1994) Structure at 2.8A
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deduced from the gene sequence, J. Biol. Chem. 261, 14896-14901.
Page 9.5
K.B. Axelsen and M.G. Palmgren (1998) Evolution of substrate specificities in the P-type
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Page 9.7
Fundamental Principles
of Membrane Biophysics
David Njus
© D. Njus, 2000
CHAPTER 10: FACILITATED DIFFUSION
OH
N C
NO2
C N NH OCF3
N C
NO2
Carbonylcyanide p-trifluoromethoxyphenyl
2,4-Dinitrophenol hydrazone (FCCP)
(DNP)
Cl
O
C NH NO2 N C Cl
H3 C C N NH
C OH Cl
CH3 CH 3
N C
5-Chloro-3-tert-butyl-2'-chloro-4'-nitrosalicylanilide Carbonylcyanide m-chlorophenyl
(S-13) hydrazone (CCCP)
Figure 10.1. Structures of some protonophores
anionic form. Of course, they can also pass across the membrane in their uncharged
(protonated) form, so they carry H+ across the membrane and bring H+ concentrations on
Page 10.1
the two sides of the membrane to electrochemical equilibrium. In terms of structure, the
protonophores are generally aromatic compounds that are both hydrophobic and capable of
distributing the negative charge over a number of atoms in the molecule. Structures of
some protonophores are shown in Figure 10.1.
Neutral ionophores generally have a cyclic structure so they are effectively
hydrophobic doughnuts capable of complexing a cation in the hole. Valinomycin, for
example, is a ring composed of three units each of D-hydroxyisovalerate, L-valine, L-
lactate and D-valine linked by alternating peptide and ester bonds (Figure 10.2).
Valinomycin complexes K+, Rb+ or Cs+, but Na+ and Li+ are too small to be effectively
bound. The selectivity for K+ is 10,000 times greater than the selectivity for Na+.
Valinomycin will cross the membrane either with or without a bound ion so it carries
charge across the membrane. Ion transport mediated by valinomycin, therefore, depends
upon the membrane potential. Moreover, valinomycin will create a membrane potential by
transporting capacitative charge.
O N
N
O N
O O O O O O
O O O O O
N O O O
O O
N O O N
O O O
O O O O O O
O N O
N O
N O
Dicyclohexyl-18-crown-6
Enniatin B
Valinomycin
Figure 10.2. Some neutral ionophores
Unlike the neutral ionophores, the carboxylic ionophores have a linear structure
with a carboxyl group on one end and one or two hydroxyls on the other (Figure 10.3).
They cyclize by head-to-tail hydrogen bonding and will cross the membrane with the
carboxyl group either protonated or complexed to an ion. Nigericin, for example, will
cross the membrane carrying either H+ or K+. It functions, therefore, as a K+/H+ exchanger.
Because nigericin does not carry a net charge across the membrane, transport is not
affected by the membrane potential nor does it contribute to the creation of a membrane
potential.
Page 10.2
Section 10.2. Transporters
Integral membrane proteins which catalyze facilitated diffusion differ from
ionophores in some important ways. First, the proteins span the membrane creating a
pathway for facilitated diffusion. They do not diffuse across the membrane as do
ionophores. Although transporters are sometimes called carriers, the latter term suggests a
protein that binds to a substrate and moves with it through a medium, not a protein through
which the substrate moves. Therefore, the term carrier may be applied to ionophores but
should not be used for transporter proteins.
A second important characteristic of transporters is that they are inserted into the
membrane with a fixed orientation. Therefore, whereas the binding process for ionophores
is the same on both sides of the membrane, transporters may be asymmetric. In particular,
the binding constants observed on the two sides of the membrane may be quite different.
In fact, for transporters to achieve maximum efficiency, the transporter should have a
binding constant higher than the expected substrate concentration on one side of the
membrane and lower than the expected concentration on the other side. This will allow the
transporter to bind the substrate on one side and release it on the other.
Finally, transporters customarily facilitate coupled transport of two or more
substrates. That is, like nigericin, transporters facilitate either exchange diffusion of two or
more substrates or codiffusion of two or more substrates. For example, the sodium/hexose
transporter, commonly found in the plasma membrane of animal cells, mediates
codiffusion of Na+ and a hexose molecule (glucose) into the cell (Figure 10.4). As we shall
see, this coupled transport is perhaps the most important mechanism for transporting
substances across biological membranes.
Page 10.3
Outside Inside
Membrane
Na +
(10.2) ∆G = RT ln ([H+]in/[H+]out) + F ψm
By setting ∆G = 0, we obtain
Page 10.4
(10.5) [H+]in/[H+]out = [K+]in/[K+]out
Nigericin will reach equilibrium when the [H+] and [K+] gradients are proportional.
For the sodium/hexose transporter,
Page 10.5
Multiplying both sides of equation 10.12 by k2K(Co - Ci) gives
- TCi(k2K-k2KCo/Ci)(Ci+K)
k1k2KT(Co-Ci)
(10.15) J =
(k1Co + k2K)(Ci+K) + (k1Ci + k2K)(Co+K)
This expression gives us the steady-state flux in terms of three parameters: k1T, K, and the
ratio k1/k2. If we restrict our consideration to the initial velocity of transport (Ci = 0), then
equation 10.15 reduces to
If we define
then
Initial velocity of transport, therefore, can be described by only two parameters, Jmax and
Km. As in enzyme kinetics, Jmax is the velocity at infinite substrate concentration; Km is the
substrate concentration giving one-half the maximum velocity. Note that the Km measured
for initial velocity of transport is equal to K, the binding constant for the substrate, only if
the rate constants (k1 and k2) are about equal.
Page 10.6
Outside Membrane Inside
TC o TC i
Co Ci
To Ti
FCCP CCCP
k1 10,000 sec-1 12,000 sec-1
k2 700 sec-1 175 sec-1
pK 6.0 5.9
First, note that protonation reactions occur on a time scale of 1011 sec-1 so the
transmembrane rates (k1 and k2) are clearly limiting. Second, note that the rate constants
for FCCP are larger than those for CCCP consistent with the more potent protonophoric
activity of FCCP. The simple model shown in Figure 10.5 applies to protonophores with
two restrictions. First, it applies only in the absence of a membrane potential since a
membrane potential will change the rate constants for the movement of the anionic species.
In fact, because the membrane potential will facilitate the movement of the anionic species
in one direction and slow its movement in the other, k2 will not be the same in both
directions. This introduces an additional parameter. Second, the model applies only for
short time periods. Because the ionophore slowly partitions between the aqueous and
membrane phases, the membrane-bound concentration may not remain constant over
longer time periods.
References
R. Benz and S. McLaughlin (1983) The molecular mechanism of action of the proton
ionophore FCCP (carbonylcyanide p-trifluoromethoxy phenylhydrazone), Biophys.
J. 41, 381-398.
Page 10.7
J. Kasianowicz, R. Benz and S. McLaughlin (1984) The kinetic mechanism by which
CCCP (carbonyl cyanide m-chlorophenylhydrazone) transports protons across
membranes, J. Membrane Biol. 82, 179-190.
R.I. Macey (1978) Mathematical models of membrane transport processes, in Membrane
Physiology (T.E. Andreoli, J.F. Hoffman, and D.D. Fanestil, eds.), Plenum, New
York, pp. 125-146.
S.G.A. McLaughlin and J.P. Dilger (1980) Transport of protons across membranes by
weak acids, Physiol. Rev. 60, 825-863.
B.C. Pressman (1973) Properties of ionophores with broad range cation selectivity, Fed.
Proc. 32, 1698-1703.
B.C. Pressman (1976) Biological applications of ionophores, Ann. Rev. Biochem. 45, 501-
530.
B.C. Pressman and M. Fahim (1982) Pharmacology and toxicology of the monovalent
carboxylic ionophores, Ann. Rev. Pharmacol. Toxicol. 22, 465-490.
Page 10.8
Fundamental Principles
of Membrane Biophysics
David Njus
© D. Njus, 2000
CHAPTER 11: COUPLED TRANSPORT
In 1961, Peter Mitchell (1961) proposed that the H+ concentration gradient drives
the synthesis of ATP. In the same year, Crane (1965) showed that the sodium
concentration gradient drives sugar and amino acid transport across epithelial cell plasma
membranes. In the years since, it has become evident that ion concentration gradients are
an important energy store in all cells. Ion pumps are therefore crucial transducers that
transform chemical-bond energy into electrochemical potential energy. Coupled transport,
in turn, uses the electrochemical potential of one substrate to drive the transport of a
second.
Page 11.1
large, the capacity of the system is low. Ca2+ concentration gradients would be greatly
perturbed by the transport of millimolar concentrations of metabolites. The H+ ion is
anomalous because it is normally present intracellularly at very low concentrations (10-7
M). Although this is necessary to reduce dissipation of the gradient, it might be expected
to adversely affect capacity. H+, however, is unusual in that its concentration is buffered
and the capacity of the system is determined not by the absolute H+ concentration but by
the buffering capacity. Because the cytosol is well buffered, the capacity of the H+ gradient
is in the millimolar range eventhough the H+ concentration is micromolar or less.
The concept of a master pump is significant because it suggests a likely mechanism
for all transport systems in a given membrane once the master pump has been identified.
The master pump concept raises some interesting questions. First, why is transport
coupled to a single master pump? A number of possible reasons can be imagined.
Coupled transport may be more efficient than independent pumps. Ion gradients generally
store smaller packets of energy than ATP. By choosing the proper coupling stoichiometry,
the energy required for transport can be matched more precisely, thereby using energy more
efficiently. Coupled transporters may be inherently more efficient than pumps. An
indication that this may be so is provided by bacteria. Under anaerobic conditions, some
cells produce ATPases for transporting amino acids. However, under aerobic conditions,
the cells dispense with the amino acid pumps and transport amino acids by coupling
transport to the H+ gradient. Coupling transport to a single master pump may also serve a
control function. This is particularly evident in the case of sequential coupled transport
(tertiary and quaternary transport). For example, the mitochondrion does not need to
import citrate as a substrate for the TCA cycle if no phosphate is available for
phosphorylation. Because citrate transport is coupled to the phosphate gradient, citrate
transport automatically stops when phosphate is depleted.
There are two reasons why a transport system might not be coupled to the master
pump. First, if the transport system has a high capacity itself, it may adversely affect the
ion gradients established by the master pump. Second, if the transported substrate serves a
regulatory function, then it may be desirable to control its concentration separately.
Exceptions to the master pump concept may exist for both of these reasons. The K+/H+
ATPase is a possible example of an exception for the reason of high capacity. The ATPase
must transport large quantities of H+ into the lumen of the gut and kidney. If H+ movement
were coupled to the Na+/K+ ATPase, it might radically affect the magnitude of the Na+
gradient. The Ca2+ ATPase may be an exception for the reason of independent regulation.
The Ca2+ concentration serves a regulatory function in animal cells, so its concentration
should not be dependent on the size of the Na+ gradient.
Ion pumps may catalyze a net transport of charge. Because this increases charge
separation across the membrane, it is termed electrogenic. Coupled transport may also
produce a net transfer of charge (non-neutral exchanges). These transport systems are
normally driven by the membrane potential, however. Therefore, these transporters tend to
dissipate rather than create a membrane potential. For that reason, they should be termed
electromotive or electrically dissipative rather than electrogenic.
Page 11.2
animal cells. The Na+/K+ ATPase transports both Na+ and K+ and, in principle, the K+
gradient could also be used for transport. In this regard, it is significant that the membrane
potential in animal cells is usually negative inside. This electrical potential adds to the
chemical potential gradient for Na+ but nearly cancels the potential gradient for K+. Thus
the Na+/K+ ATPase puts the chemical energy into the Na+ gradient rather than the K+
gradient. Since K+ ions can contribute little energy for transport, it is not surprising that K+
is usually not involved in coupled transport across the plasma membrane. For example, in
the squid axon, the membrane potential is -90 mV, the K+ equilibrium potential is -102
mV, and the Na+ equilibrium potential is +50 mV. A K+ ion can contribute only 12 mV of
energy to transport whereas a Na+ ion can contribute +140 mV. This illustrates how the
energy stored by the Na+/K+ ATPase is put mainly into the Na+ ions and not into K+.
ATP
3 Na+ Na+
+ Glucose
2K
ADP + Pi
Figure 11.1. Coupled transport across the animal cell plasma membrane.
In animal cell plasma membranes, a glucose transporter uses the Na+ gradient to
bring glucose into the cell. It does this by mediating the cotransport of 1 glucose molecule
and 1 Na+ ion. The energetics of this coupled transport may be analyzed by applying the
same logic used earlier. The total free energy change is the sum of the electrochemical
potential changes of all transported species (equation 10.1):
Both glucose (G) and Na+ are transported from outside to inside, so they may both be
assigned a stoichiometry of +1. Then, at equilibrium,
Consequently, the glucose concentration gradient will depend on the sodium gradient and
on the membrane potential as
Page 11.3
Section 11.3. Inner Mitochondrial Membrane
The electron transfer chain in the mitochondrial inner membrane uses energy
derived from substrate oxidation to pump H+ out across the membrane. This gradient, of
course, is used to drive the synthesis of ATP. It is also used to transport many metabolites
across the inner mitochondrial membrane. ATP and ADP cross the inner mitochondrial
membrane via a transporter catalyzing ATP/ADP exchange. ATP carries one more
negative charge than ADP, so the exchange is electrically dissipative. The membrane
potential (inside negative) generated by H+ transport, will drive ATP/ADP exchange in the
direction of ATP efflux and ADP influx.
PO4- enters mitochondria via PO4-/OH- exchange. This is an electroneutral exchange but it
will respond to the H+ concentration gradient by virtue of the inverse relationship between
H+ and OH- concentrations. Consequently, PO4- will accumulate in mitochondria because
of the higher internal OH- concentration.
From equations 11.4 - 11.6, it should be apparent that the electrochemical potential of one
proton drives the uptake of ADP and phosphate into the mitochrondrial matrix and the
export of ATP from the matrix. The electrical part of ∆µ H+ drives ADP uptake and ATP
export, while PO4- uptake is driven by the chemical part of ∆µH+.
P
i
H ++ 1/2 O2
NADH
-
OH
H+ ATP 4-
NAD +
+ H 2O
3-
ADP
Page 11.4
Section 11.4. Secretory Vesicle Membranes
Secretory vesicle membranes have a vacuolar ATPase that pumps H+ into the
intravesicular space. Consequently, we would expect transport across secretory vesicle
membranes to be coupled to the H+ gradient. Catecholamines (epinephrine, norepinephrine
and dopamine) are indeed transported across the secretory vesicle membrane via a
catecholamine/proton exchange. The stoichiometry of this exchange is 2 protons per
protonated amine so the equilibrium catecholamine gradient is
The equilibrium gradient depends on the square of the H+ gradient because two protons are
exchanged for each amine. Because the charge on the amine cancels the charge on one of
the two protons, there is a net movement of only one charge in the exchange. For this
reason, the catecholamine gradient depends on the membrane potential only to the first
power.
ATP
RNH 3+
H+
2 H+
ADP
+ Pi
Page 11.5
References
Bell, G.I., C.F. Burant, J. Takeda, and G.W. Gould (1993) Structure and function of
mammalian facilitative sugar transporters, J. Biol. Chem. 268, 19161-19164.
Crane, R.K., Miller, D., and Bihler, I. (1961) in Symposium on Membrane Transport and
Metabolism (A. Kleinzeller and A. Kotyk, ed.), Academic Press, London, p. 439.
Crane, R.K. (1965) Na+-dependent transport in the intestine and other animal tissues, Fed.
Proc. 24, 1000-1006.
Mitchell, P. (1961) Coupling of phosphorylation to electron and hydrogen transfer by a
chemi-osmotic type of mechanism, Nature 191, 144-148.
Schuldiner, S. (1994) A molecular glimpse of vesicular monoamine transporters, J.
Neurochem. 62, 2067-2078.
West, I.C. (1980) Energy coupling in secondary active transport, Biochim. Biophys. Acta
604, 91-126.
Page 11.6
Fundamental Principles
of Membrane Biophysics
David Njus
© D. Njus, 2000
CHAPTER 12: ENERGY COUPLING
where J is the flow and X is the force driving that flow. Conservation of energy requires
that output power not exceed input power:
Consequently, 1 ≥ η ≥ 0.
The flows are related by the stoichiometry of coupling n:
(12.3) n = Jout/Jin
(12.4) η = - n[Xout/Xin]
Maximum efficiency is attained when η = 1, but this is the equilibrium condition (JinXin = -
JoutXout). Therefore, no net flow will be produced. Consequently, productive energy
coupling with maximum efficiency requires that η be close to but not equal to one, and that
the coupled flows be close to but not at equilibrium.
As discussed in Chapter 9 (equation 9.8), the free energy change for this reaction is
Page 12.1
The free energy change will be less than zero when Edonor < Eacceptor. Therefore, reducing
equivalents will be transferred from carriers with lower reduction potentials to carriers with
higher reduction potentials and this will generally be in the direction of increasing E°.
At equilibrium, ∆Gredox = 0 and the ratio of products to reactants is equal to the
equilibrium constant K12. Therefore,
where Z = 1011 M-1s-1. In practice, f12 is usually about equal to 1. A significant implication
of the Marcus theory is that electron transfer is an intrinsically non-specific process and
will occur most rapidly between the lowest-potential electron donor and the highest-
potential electron acceptor. Electron transfer, therefore, is not compatible with the
sequential redox reactions of a redox chain. In fact, electron carriers tend to be avoided in
biological systems because their reactions are uncontrolled.
Instead, biological redox reactions employ organic compounds that donate or accept
hydrogen atoms. These compounds do not react spontaneously so their redox reactions can
be controlled by enzymes. The enzymes that react with these organic hydrogen-atom
carriers, however, are generally metalloproteins. The metal ligand will accept and donate
only electrons, not hydrogen atoms. The active site of the metalloenzyme, therefore, must
provide its substrate (the hydrogen-atom carrier) with a way to exchange a proton as well
as an electron. We will call this phenomenon concerted proton/electron transfer.
Concerted proton/electron transfer implies that biological redox reactions will
naturally release or consume H+. The generation of proton gradients, therefore, is a natural
consequence of the vectorial organization of these reactions. Because electron transfer
reactions are avoided in biology, terminology such as “electron transfer chain” is
misleading. Instead, we will refer to the respiratory chain in mitochondria or to redox
chains.
Page 12.2
Section 12.3. The Secretory-Vesicle Ascorbic Acid-Regenerating Chain
A good, simple example of a biological redox chain is the ascorbic acid-
regenerating system in secretory vesicles. The function of this system is to provide
reducing equivalents for redox reactions occurring inside the secretory vesicles. Two
reactions, those catalyzed by dopamine β-monooxygenase (DβM) and peptidylglycine α-
amidating monooxygenase (PAM), are especially significant. DβM converts dopamine to
norepinephrine and mediates catecholamine biosynthesis in adrenal chromaffin cells, in
peripheral sympathetic nerve endings and in noradrenergic neurons in the central nervous
system. PAM and an associated lyase lead to amidation of the carboxyl termini of many
peptide hormones including vasopressin, oxytocin, VIP, neuropeptide Y, α-MSH,
substance P, calcitonin and gastrin.
The monooxygenases both incorporate one atom from O2 into the substrate and
reduce the second oxygen atom to H2O. The reducing equivalents are provided by
intravesicular ascorbic acid (vitamin C) which in turn is oxidized to the radical anion,
semidehydroascorbate. The intravesicular ascorbate is recycled by importing reducing
equivalents across the vesicle membrane through cytochrome b561 (Figure 12.1).
Cytochrome b561, which spans the secretory-vesicle membrane, is reduced in turn by
cytosolic ascorbic acid. This transport of electrons into the vesicles is driven by both the
membrane potential (interior positive) and the pH gradient (interior acidic) generated by a
V-type H+-translocating ATPase in the vesicle membrane. The pH-gradient favors inward
electron flow because the midpoint potential of ascorbate is pH-dependent being higher at
the lower internal pH. Thus, contrasting with the mitochondrial respiratory chain in which
H+ is transported using energy from redox reactions, the secretory-vesicle system drives the
redox reaction using energy from the proton gradient.
Page 12.3
H2 COH H2 COH H2 COH
H COH pK1 H COH pK2 H COH
O O O
O -H+ O -H+ O
H H +H+ H
+H+
-
HO OH HO O- O O-
AH2 AH- A=
+H
E°1 +e- -e- -H +e- -e- E°2
H2 COH H2 COH
pKr H COH
H COH
O O -H+ O O
. .
-
H +H+ H
HO O O O
AH . A-.
H2 COH
H
KH H COH
H
HO O O O
-H2O O
H H
OH +H2O
O
H OH OH O O
AH2O A
Figure 12.2. Interconversion of ascorbate species. Protonation reactions are shown
horizontally and electron-transfer reactions are shown vertically.
Page 12.4
To understand how this system works, it is important to understand that ascorbic
acid functions as a donor of single hydrogen atoms. At physiological pH, ascorbic acid
exists predominantly as a monoanion (AH-). Similarly, the oxidized form occurs as a
radical anion (A-) because this form is stabilized by its capacity to distribute the unpaired
electron over a number of atoms. The fully oxidized form, dehydroascorbate, is not
normally formed because that requires formation of a very unfavorable reaction
intermediate (A). Consequently, cytochrome b561 must react with ascorbic acid and its
radical anion by exchanging the equivalent of a single hydrogen atom. Because the metal
ligand (Fe) will only accept an electron, there must be a mechanism to facilitate ascorbate
deprotonation. This implies that cytochrome b561 reacts with
ascorbate/semidehydroascorbate by concerted proton/electron transfer. Because the
reaction involves only one reducing equivalent and causes no other chemical changes, it is
an especially simple example of this kind of redox reaction.
The distinction between concerted H+/e- transfer and e- transfer is illustrated by
comparing cytochrome b561 with cytochrome c. The rate at which ascorbate reduces
cytochrome c is relatively slow at neutral pH, strongly pH-dependent, and does not saturate
at high ascorbate concentrations. By contrast, the rate of cytochrome b561 reduction is
faster, only weakly pH dependent, and saturates at ascorbate concentrations over about 1
mM. This implies that cytochrome c is reduced by the ascorbate dianion (A=) by outer
sphere electron transfer whereas cytochrome b561 is reduced by the ascorbate monoanion by
concerted H+/e- transfer.
A probable but unproven implication of concerted H+/e- transfer is that the substrate
binding site is sufficient to make the substrate reactive. By creating a mechanism for
proton transfer, the binding site:substrate complex becomes capable of engaging in electron
transfer reactions. This means that metalloproteins can be thought of as two (or more)
separate electron transfer centers: the metal and the bound substrate(s). The bound
substrate will normally exchange electrons with the metal because that pathway is
available, but the bound substrate may also react with other redox centers that may happen
to be in the vicinity. Consequently electrons may occasionally go astray, for example,
reducing O2 to superoxide (O2-).
Cytochrome b561 reacts with ascorbate on both sides of the membrane and therefore
must possess two ascorbate binding sites. The site on the cytoplasmic side probably
contains a histidine residue, because histidine modification inhibits reduction of
cytochrome b561 by external ascorbate. Cytochrome b561 has recently been cloned and
sequenced and has a molecular weight of 30,061. Hydropathy analysis suggests that the
protein has six transmembrane domains with both amino and carboxyl termini being on the
cytoplasmic side of the membrane. The protein contains seven histidine residues, two of
which act as ligands to the heme. The other five are candidates for the ascorbate binding
sites which mediate concerted H+/e- transfer.
Page 12.5
chain into three segments: the NADH oxidase/CoQ reductase, CoQ oxidase/cytochrome c
reductase, and cytochrome c oxidase. Let us consider each of these components in turn.
If the two processes are allowed to come to equilibrium, ∆Gox + n ∆GATP = 0, where n is
the stoichiometry of coupling. By measuring the equilibrium concentrations of the various
reactants, Rottenberg and Gutman could calculate ∆Gox and ∆GATP and then determine n.
They found a stoichiometry of 4/3. Since the F1Fo ATPase transports 3H+/ATP, this
segment of the respiratory chain must transport 4 H+ per pair of reducing equivalents.
Page 12.6
The CoQ oxidase/cytochrome c reductase segment of the respiratory chain
transports H+ across the inner mitochondrial membrane through a process that Peter
Mitchell termed the Q Cycle. This mechanism is diagrammed in Figure 12.3. It depends
upon two reactions occurring on opposite sides of the inner mitochondrial membrane. On
the matrix side, ubiquinone is reduced by two reducing equivalents, one taken from
cytochrome b562 and the other taken from an iron sulfur center:
The reduced quinone is uncharged and hydrophobic and will diffuse to the other side of the
membrane where it reduces cytochrome c1 and cytochrome b566:
Intermembrane Matrix
Space FeS
Q Q
QH 2 QH 2
Cyt c 1
Cyt c
Page 12.7
pK1 pK2
OH O- O-
CH3O + CH3O
CH3 -H+ CH3O CH3 -H CH3
QH2 QH- Q=
O pKr O
CH3O CH3 CH3O CH3
. -H+ .
CH3O R + CH3O
- R
+H
OH O
QH . Q-.
O
CH3O CH3
CH3O R
O
Q
Figure 12.4. Interconversion of quinone species. Protonation reactions are shown
horizontally and electron-transfer reactions are shown vertically.
Page 12.8
This results in the net transfer of 2 H+ across the membrane. Because one of the two
reducing equivalents is recycled by cytochromes b562 and b566, a pair of reducing
equivalents passing completely through the Q cycle will cause 4 H+ to be pumped out
across the mitochondrial inner membrane.
Note that the quinone is a carrier of two hydrogen atoms and would be expected to
react with the metalloproteins on both sides of the membrane by concerted proton/electron
transfer. The requirement for a specific active site for each reaction is indicated by the fact
that the reactions are blocked by different compounds; myxothiazol inhibits quinone
oxidation on the outside and antimycin inhibits quinone reduction on the inside.
Page 12.9
which O2 is reduced to 2 H2O. Cytochrome oxidase catalyzes a series of electron transfer
reactions and only the reduction of O2 involves H+. Consequently, the cytochrome oxidase
redox reactions do not naturally lead to formation of a proton gradient. Instead,
cytochrome oxidase actually functions as a proton pump. Redox energy is captured and
used to transport H+ across the inner mitochondrial membrane. H+ transport is believed to
be linked specifically to the flow of electrons through the heme a/CuA region of
cytochrome oxidase.
Having now considered the entire mitochondrial respiratory chain, it is possible to
assess the theoretical energy yield of oxidative phosphorylation. Assuming that the ATP
synthase has a stoichiometry of 3 H+/ATP and that 1 H+/ATP is consumed for transport of
ATP, ADP, and inorganic phosphate, the following stoichiometries hold:
NADH/succinate 4 1.0
Succinate/cytochrome c 4 1.0
Cytochrome c/O2 2-4 0.5-1.0
The passage of two reducing equivalents down the entire mitochondrial respiratory chain
will cause 1 atom of O to be reduced to H2O. The theoretical P/O ratio (molecules of ATP
formed per atom of O reduced) is 2.5 - 3.0. According to long-held dogma, the theoretical
P/O ratio is 3 but this was established before it was recognized that the ratio did not have to
be an integer. The observed P/O ratio is typically between 2 and 3.
The observed P/O ratio is expected to be less than the theoretical value because of
H+ leakage or poor coupling. The degree of coupling is indicated by the phenomenon of
respiratory control. In the absence of ADP, mitochondria consume O2 at a slow but
measurable rate. This so-called state 4 rate is the rate at which reducing equivalents may
be transferred down the respiratory chain in the absence of ATP synthesis. Presumably,
this redox flow generates a large ∆µH+ and the energy needed to pump H+ against this
substantial gradient slows the flow of reducing equivalents. Another way to look at this is
to recognize that, in the steady state, electron flow may pump protons only as fast as the
protons come back across the membrane. When ADP is added, the rate of O2 consumption
increases dramatically. This so-called state 3 rate is faster because ∆µH+ is dissipated by
ATP synthesis. Thus, electron flow may pump protons as fast as H+ are consumed by the
ATP synthase (F1Fo ATPase). The respiratory control ratio is the ratio of the rate of O2
consumption in state 3 to the rate of O2 consumption in state 4. Generally this ratio lies
between 3 and 15. A high ratio indicates tight coupling between electron flow and ATP
synthesis. A low ratio signifies poor coupling. Complete uncoupling (for example, by
adding an uncoupler) will accelerate state 4 respiration to the rate of state 3 respiration and
will cause the respiratory control ratio to drop to 1.
Page 12.10
solved. It was one of the first membrane structures for which this was accomplished, and
for it Diesenhofer and Michel shared the 1988 Nobel Prize.
The redox chain incorporates a reaction center which takes reducing equivalents
from cytochrome c2 (a soluble protein), invests energy from light, and then reduces
ubiquinone. Reducing equivalents from ubiquinone return to cytochrome c2 by passing
through b and c cytochromes. In the process, protons are pumped across the membrane.
The proton gradient can be used to make ATP as in mitochondria or chloroplasts.
Alternatively, it can be used to drive reverse electron flow from succinate to NADH.
Cytochrome c2 can pass reducing equivalents to the reaction center or to a cytochrome
oxidase which reduces O2. Thus, the redox chain can create a proton gradient by a cyclic
photosynthetic pathway in the light or it can create a proton gradient by oxidizing succinate
or NADH aerobically in the dark.
The photosynthetic reaction center incorporates four molecules of
bacteriochlorophyll, two of pheophytin and two of ubiquinone. The structure has two fold
symmetry. A bacteriochlorophyll dimer near the outer surface of the membrane donates an
electron upon absorbing light. The electron passes crosses the membrane via a
bacteriochlorophyll and pheophytin molecule on one side of the reaction center. The
pigment molecules on the other side apparently do not function in the photosynthetic
process. The electron finally passes to a quinone on the inside of the membrane. This
quinone (QA) is reduced to the semiquinone but apparently does not become fully reduced.
QA passes the electron to QB, accepts a second electron from the reaction center, and passes
it to QB to form the fully reduced dihydroquinone. The dihydroquinone (QH2B) then
dissociates from the reaction center and is replaced by an oxidized quinone from the
quinone pool.
The quinone reduction results in proton uptake and the nature of the proton uptake
mechanism has received some attention. Note that the oxidized quinone Q may be reduced
to the semiquinone by electron transfer, because the radical species have a pK near
neutrality so both Q- and QH. are formed readily. The second reduction step must form
QH2 because the pK for ionization of the species is greater than 12. Consequently, we
might imagine that QH2 will form by reduction of QH. by concerted proton/electron
transfer.
Page 12.11
+
Rhodopseudomonas Redox Chain NAD
Q = Ubiquinone
BChl = bacteriochlorophyll Y Succinate
Q
Cyt b
hν
Cyt c 1
Cyt c 2
BChl
Cyt a/a 3
1/2 O 2
Mitochondrial Respiratory Chain
NADH
FeS Succinate
Cyt b
Cyt c 1
Cyt c
Cyt a/a 3
1/2 O 2
PQ = plastoquinone
PC = plastocyanin
Fd = ferredoxin X
Y
Fd
PQ
NADP+
Cyt b559
hν
hν
Cyt f
H 2O PC
P 680 P700
Photosystem II Photosystem I
Page 12.12
Section 12.6 The Photosynthetic Redox Chain
The thylakoid membranes in chloroplasts accomplish the following so-called light
reactions of photosynthesis:
The free energy of NADP+ reduction is about 40 kcal/mole and that of ATP synthesis is
about 10 kcal/mole. The energy obtainable from a single photon of red light is about 40
kcal/mole. The efficiency of energy conversion in photosynthesis is about 40%, but this
means that the energy from four photons of light are needed to drive one pair of reducing
equivalents through the redox chain. Each electron must receive the energy from two
photons and this is accomplished by placing two photosystems in the redox chain.
The light energy is collected by antenna pigments in the chloroplast and transferred
to the reaction centers. To make photosynthesis proceed efficiently, it is important that
light be distributed evenly between the two photosystems so that they run at the same
speed. Plants accomplish this by using the redox state of the quinone pool to regulate the
distribution of light energy between the two photosystems. If the quinone pool is too
reduced, then PS I is operating too slowly and more light needs to be diverted to it.
Conversely, if the quinone pool is too oxidized, then more light must be shunted to PS II.
This is accomplished by phosphorylation control of light harvesting protein (Allen et al.,
1981). If the quinone pool is reduced, a kinase is activated increasing phosphorylation of
the LHCP. This directs more of the light energy to PS I. If the quinone pool becomes too
oxidized, the kinase is inactivated and the LHCP is dephosphorylated by a phosphatase. A
greater fraction of the absorbed quanta are then shunted to PS II.
Photosystem II is quite similar to the reaction center of bacterial photosynthesis in
Rhodopseudomonas. Consequently, the solution of the structure of the bacterial reaction
center has also illuminated the mechanism of photosystem II.
References
J.F. Allen, J. Bennett, K.E. Steinback and C.J. Arntzen (1981) Chloroplast protein
phosphorylation couples plastoquinone redox state to distribution of excitation
energy between photosystems, Nature 291, 25-29.
P. Brzezinski (1996) Internal electron-transfer reactions in cytochrome c oxidase,
Biochemistry 35, 5611-5615.
S.I. Chan and P.M. Li (1990) Cytochrome c oxidase: Understanding Nature's design of a
proton pump, Biochemistry 29, 1-12.
K. Krab and M. Wikstrom (1987) Principles of coupling between electron transfer and
proton translocation with special reference to proton translocation mechanisms in
cytochrome oxidase, Biochim. Biophys. Acta 895, 25-39.
R.A. Marcus and N. Sutin (1985) Electron transfers in chemistry and biology, Biochim.
Biophys. Acta 811, 265-322.
H. Michel and J. Deisenhofer (1988) Relevance of the photosynthetic reaction center from
purple bacteria to the structure of photosystem II, Biochemistry 27, 1-7.
Page 12.13
P. Mitchell (1961) Coupling of phosphorylation to electron and hydrogen transfer by a
chemi-osmotic type of mechanism, Nature 191, 144-148.
P. Mitchell (1975) Protonmotive redox mechanism of the cytochrome b-c1 complex in the
respiratory chain: Protonmotive ubiquinone cycle, FEBS Lett. 56, 1-6.
P. Mitchell (1976) Possible molecular mechanisms of the protonmotive function of
cytochrome systems, J. Theoret. Biol. 62, 327-367.
P. Mitchell (1979) Keilin's respiratory chain concept and its chemiosmotic consequences,
Science 206, 1148-1159.
D. Njus and P.M. Kelley (1993) The secretory-vesicle ascorbate-regenerating system: A
chain of concerted H+/e- transfer reactions, Biochim. Biophys. Acta 1144, 235-248.
M.Y. Okamura and G. Feher (1992) Proton transfer in reaction centers from photosynthetic
bacteria, Ann. Rev. Biochem. 61, 861-896.
H. Rottenberg and M. Gutman (1977) Control of the rate of reverse electron transport in
submitochondrial particles by the free energy, Biochemistry 16, 3220-3227.
B.L. Trumpower and R.B. Gennis (1994) Energy transduction by cytochrome complexes in
mitochondrial and bacterial respiration: The enzymology of coupling electron
transfer reactions to transmembrane proton translocation, Ann. Rev. Biochem. 63,
675-716.
I.C. West, P. Mitchell and P.R. Rich (1988) Electron conduction between b cytochromes of
the mitochondrial respiratory chain in the presence of antimycin plus myxothiazol,
Biochim. Biophys. Acta 933, 35-41.
J.R. Winkler, B.G. Malmstrom, and H.B. Gray (1995) Rapid electron injection into
multisite metalloproteins: intramolecular electron transfer in cytochrome oxidase,
Biophys. Chem. 54, 199-209.
Page 12.14
Fundamental Principles
of Membrane Biophysics
David Njus
© D. Njus, 2000
i
Page 13.1
i
membrane potential across the apical membrane. The potential on the serosal side is the
transepithelial potential (ψtep). The membrane potential across the basolateral membrane is then
Serosal Mucosal
(Blood) (Lumen)
I baso
K+
ATP 2 K +
I api
3 Na +
ADP + Pi Na +
I para
ψ ψ ψ =0
tep api
Basolateral Apical
Membrane Membrane
The apical membrane is permeable to Na+ but not K+, so the current across the apical membrane
is
ψNa is the Na+ equilibrium potential across the apical membrane, and RNa is the resistance to the
Na+ current across the apical membrane.
The basolateral membrane is permeable to K+ but not Na+, so the current across the
basolateral membrane is
Page 13.2
i
This shows that ψapi lies between the apical Na+ equilibrium potential (ψNa) and the basolateral
K+ equilibrium potential (ψK). If RNa = 0, then Na+ will be in equilibrium across the apical
membrane and ψapi = ψNa. At the other extreme, if RK = Rpara = 0, then K+ will be in equilibrium
across the basolateral membrane, and ψbaso = ψK. The transepithelial potential will be zero, so
ψapi = ψbaso = ψK.
The transepithelial potential (ψtep) is a little smaller than the difference between the apical
Na and basolateral K+ equilibrium potentials (ψNa - ψK).
+
Page 13.3
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Serosal Mucosal
(Blood) (Lumen)
K+
ATP 2 K + Hexose
3 Na+ ADP + Pi Na +
Hexose
+10 mV -40 mV 0 mV
Basolateral Apical
Membrane Membrane
The oxyntic cells of the gastric mucosa are responsible for secreting HCl to acidify gastric
juice in the stomach. Because gastric juice is extremely acidic, H+ and Cl- are moved against
large concentration gradients, and this requires considerable energy. Estimating the H+ and Cl-
concentrations in gastric juice and blood as shown below, the transepithelial ∆µ for H+ and Cl-
may be calculated:
The energy for H+ and Cl- transport is satisfied by a K+/H+ ATPase located in the apical
membrane. This enzyme actively pumps H+ against the large H+ concentration gradient across
the apical membrane. The concomitant K+ influx neutralizes charge movement. The K+ gradient
is then used to drive Cl- into the lumen via an electroneutral K+/Cl- cotransport. Cl- influx across
the basolateral membrane is driven by the Na+ gradient generated by the Na+/K+ ATPase. Na+
influx is coupled to Cl- influx via parallel transporters: a Cl-/HCO3- exchanger and a Na+/H+
exchanger.
Page 13.4
i
Serosal Mucosal
(Blood) (Lumen)
ATP
K+ +
K
ATP 2 K +
+
ADP + Pi H
3 Na+ ADP + Pi Cl -
Na +
Cl -
H2 O +
H
Cl - K+
CO 2
HCO3 -
Basolateral Apical
Membrane Membrane
Page 13.5
i
Serosal Mucosal
(Blood) (Lumen)
K+ Cl -
ATP 2 K +
HCO 3 -
H+
H 2O
3 Na+ ADP + Pi
CO 2
+
Na
Basolateral Apical
Membrane Membrane
Serosal Mucosal
(Blood) (Lumen)
Cl - K+
ATP 2 K +
Na +
K+
2 Cl -
3 Na+ ADP + Pi
Basolateral Apical
Membrane Membrane
In the thick ascending limb of the loop of Henle, NaCl reabsorption must be achieved
without loss of water by osmosis. Na+ and Cl- are taken up from the lumen by the Na+/K+/2Cl-
6
Page 13.6
i
cotransporter which is driven by the Na+ and Cl- gradients. The Na+ concentration in the
epithelial cells is kept low by the Na+/K+ ATPase. The Cl- concentration is kept low by a Cl-
channel in the basolateral membrane. Cl- efflux through this channel is driven by the membrane
potential. A K+ channel is located in the apical membrane rather than in the basolateral
membrane. This reduces the K+ gradient across the apical membrane and prevents it from
interfering with the proper functioning of the cotransporter. The K+ channel can also cause the
transepithelial potential to be positive on the lumen side. This arrangement results in the active
transport of NaCl across the epithelium and can make the urine hypotonic relative to the blood.
The loop diuretic furosemide (Lasix) inhibits NaCl reabsorption by blocking the Na+/K+/2Cl-
cotransporter. Because it blocks K+ reuptake from the lumen, furosemide leads to K+ loss.
Amiloride, which blocks water reabsorption by acting on Na+/H+ exchange, does not do this and
is known as a K+-sparing diuretic.
Serosal Mucosal
(Blood) (Lumen)
K+
ATP 2 K +
3 Na + ADP + Pi Cl -
Na +
K+
2 Cl -
Basolateral Apical
Membrane Membrane
The Na+/K+ ATPase drives Cl- influx across the basolateral membrane, and Cl- flux across the
apical membrane then occurs passively. The symptoms of cystic fibrosis are caused by a defect
7
Page 13.7
i
in regulation of the Cl- channel resulting in inadequate H2O secretion making secretions
characteristically salty and too viscous.
Airway epithelia and the epithelia in the nephrons of the kidney are structured in similar
ways, yet airway epithelia function to secrete Cl- while the epithelia in the kidney take up Cl-.
This illustrates how the general structure of an epithelium can be adapted to achieve different
functions simply by incorporating appropriate combinations of transporters in the apical or
basolateral membranes.
References
W.B. Reeves and T.E. Andreoli (1992) Renal epithelial chloride channels, Ann. Rev. Physiol. 54,
29-
J.R. Riordan (1993) The cystic fibrosis transmembrane conductance regulator, Ann. Rev. Physiol.
55, 609-630.
B. Thorens (1993) Facilitated glucose transporters in epithelial cells, Ann. Rev. Physiol. 55, 591-
608.
E.J. Weinman and S. Shenolikar, (1993) Regulation of the renal brush border membrane Na+-
exchanger, Ann. Rev. Physiol. 55, 289-304.
M.J. Welsh (1987) Electrolyte transport by airway epithelia, Physiol. Rev. 67,1143-1184.
M.J. Welsh, M.P. Anderson, D.P. Rich, H.A. Berger, G.M. Denning, L.S. Ostedgaard, D.N.
Sheppard, S.H. Cheng, R.J. Gregory and A.E. Smith, (1992) Cystic fibrosis transmembrane
conductance regulator: A chloride channel with novel regulation, Neuron 8, 821-829.
C.S. Wingo and B.D. Cain (1993) The renal H-K-ATPase: Physiological significance and role in
potassium homeostasis, Ann. Rev. Physiol. 55,323-347.
E.M. Wright (1993) The intestinal Na+/glucose cotransporter, Ann. Rev. Physiol. 55, 575-589.
Page 13.8