Advance your skill

and knowledge to
the next level.
And keep it there.

2010 Biotechnology
& Bioprocessing
Training Programs
Intensive, innovative, hands-on laboratory training in:
• Downstream Processing of Biotechnology Products
• Techniques in Animal Cell Culture and Scale-Up Strategies
• Microbial Fermentation: Development & Scale-Up
• Gene Expression & Microarray Analysis
About Our Program Who Should Attend
Attendees who
The Biotechnology and Bioprocessing will benefit from
these courses
Training at USU’s Center for Integrated include industrial
microbiologists,
BioSystems provides training and cell biologists,
bioprocess engineers,
technical support to researchers biochemical
engineers, production
and educators from all realms of the managers, group
leaders, scientists
scientific community. Participants come in manufacturing
and R&D in the biopharmaceutical and
to our program to gain new insights biotechnology industries. This year we
have refi ned our program for academic and
and knowledge from experts covering government scientists.

theory and the newest practices.

Our experienced staff is dedicated to
illuminating every aspect of cell culture,
fermentation, and protein purification,
as well as gene expression and proteome
analysis. Most importantly, our courses
provide an opportunity for attendees
to translate theory into hands-on
experience during our cutting-edge
laboratory sessions.
About the Center
The Center for Integrated BioSystems at
Utah State University leads a progressive,
interdisciplinary effort in research, core lab
services, and educational programs serving
life science professionals. Our Center is
a consortium of faculty and technical
talent, housed in a state-of-the-art facility
on the Utah State University campus.
We collaborate with and train scientists
in academic, industrial, and national
laboratories.
Downstream Processing of Biotechnology Products
September 14-17, 2010
Do you have the knowledge
and background to make
intelligent decisions about
your downstream processes?
This offering is designed to
provide an overview of this
critical aspect of biotechnology
and equip you with the
knowledge and practical skills
to optimize your separation
and purification operations.
In-depth presentations of
the theoretical aspects of
downstream processing are
combined with unified hands-
on laboratory sessions where
the participant completes
the steps to create a finished
product from whole cells in
culture. The characterization
of the product using state-
of-the-art analytical
instrumentation is also
covered.
Registration: $1750
Early registration: $1650
Special rates are available
for academic and
government attendees.
Day 1 Day 3
Lecture: Lecture:
Introduction to downstream Chromatography:
processing Purification strategies
Principles of centrifugation Optimization
Cell disruption and protein capture
Filtration:
Laboratory: Filtration principles – conventional
and tangential flow
Cell harvest from fermentor
Cell disruption and clarification Filtration techniques –
microfi ltration, ultrafi ltration and
diafi ltration
Laboratory:
Day 2
Lecture: Tangential flow fi ltration
Protein polishing using gel fi ltration
Principles of chromatography
chromatography
Ion exchange, Hydrophobic
interaction
Affi nity chromatography, gel
fi ltration Day 4
Lecture:
Laboratory:
MS technologies in high throughput
Protein chromatography proteomics
Protein identification utilizing
MALDI-QTOF mass spectrometry
Laboratory:
Protein characterization using
MALDI-QTOF mass spectrometry
~ Adjourn at noon
Instructors:
• Peter Moore, Ph.D., GE Healthcare
• John Peltier, Ph.D., Prolexys Pharmaceuticals, Inc.
• Kamal Rashid, Ph.D., Center for Integrated BioSystems, USU
• Tim Taylor, Ph.D., Biological and Irrigation Engineering, USU
Techniques in Animal Cell Culture & Scale-up Strategies
April 13-16, 2010 / November 16-19, 2010

This course teaches modern Day 1 Day 3

Lecture: Lecture:
techniques used with
Fundamentals of cell culture, Cell Culture – single use bioreactor
mammalian cell culture. primary culture, cell transformation systems

Laboratory work includes
hands-on experience in cell
growth and subculturing,
and extensive work with
different types of bench scale
bioreactors. Also included are
lectures and laboratory work
on cloning, nuclear transfer,
and stem cell manipulations.
Registration: $1750
Early registration: $1650
Special rates are available
for academic and
government attendees.
Media design, serum, serum-free
media
Laboratory:
Laboratory safety, aseptic
techniques, culturing and
subculturing, cell viability, growth
parameters and culture expansion
for seeding reactors
Day 2
Lecture:
Application of nuclear transfer to
human medicine
Methodologies associated with
successful nuclear transfer
Stem cell research and applications
in human regenerative medicine
and agriculture
Stem cell evaluation, alkaline
phosphatase analysis
Immunohistochemistry of stem cell
surface markers
Microcarrier and hollow fiber cell
culture
Stirred-tank bioreactor design and
operations
Laboratory:
Bench top reactor setup and
preparation for sterilization
Media handling and asepsis, pH
and DO probe preparation and
calibration
Seeding the reactors
Day 4
Lecture:
Cell line characterization for
production of biologics
Rapid detection of mycoplasma in
cell culture
Laboratory:
Follow-up on the stirred-tank
reactor experiments

Laboratory:
Enucleation and nuclear cell ~ Adjourn at noon
transfer
Fusion and activation
Feeder and non-feeder layer setup
Matrigel coating
Colony picking and stem cell
maintenance

Instructors:
• Joseph Camire, Thermo Fisher
• Vikram Gossain, Ph.D., New Brunswick Scientific Co., Inc.
• Kum Park, Center for Integrated BioSystems, USU
• Kamal Rashid, Ph.D., Center for Integrated BioSystems, USU
• Lee Rickords, Ph.D., Animal, Dairy & Veterinary Sciences, USU
• Tim Taylor, Ph.D., Biological and Irrigation Engineering, USU
• Ken White, Ph.D., Animal Dairy & Veterinary Sciences, USU
Microbial Fermentation: Development and Scale-Up
March 16-19, 2010 / May 18-21, 2010 / October 19-22, 2010

This 3½ - day course Day 1 Day 3

Lecture: Lecture:
emphasizes extensive
Overview of microbial fermentation Scale-up strategies in fermentation
laboratory work and Kinetics of fermentation
processes

demonstrations in microbial Media formulations for fermentation Biomass monitor

processes
fermentation from bench scale
Laboratory:
to pilot scale. Laboratory
Recombinant protein production in
work will be supported by bench-top fermentors
Fermentor setup and sterilization
lectures and discussions on
Fed-batch strategy and
the fundamentals of microbial experimental design
Anaerobic fermentation
Laboratory:
Pilot scale (100 L) fermentors
Fermentor setup, sterilization and
preparation for inoculation
Fermentation monitoring and
control

growth, growth kinetics, strain Inoculum development, fermentor Troubleshooting issues

development, and scale-up
strategies. Participants will
gain the skills and knowledge
to design and optimize
fermentation processes in
batch and fed-batch cultures.
Registration: $1750
Early registration: $1650
Special rates are available
for academic and
government attendees.
inoculation, pH and DO preparation,
and calibration
Day 4
Lecture:
Day 2
Lecture:
Strain development and
improvement
Protein expression in bacteria
Laboratory:
Computer control of fermentation
Harvest and processing of 100 L
Design of bench-top fermentors
fermentors
Laboratory: Continuous flow centrifugation
Continuation of bench scale
fermentation-harvest
Oxygen mass transfer in 10 L
~ Adjourn at noon
fermentor
Instructors:
• Vikram Gossain, Ph.D., New Brunswick Scientific Co., Inc.
• John Poppleton, Applikon
• Kamal Rashid, Ph.D., Center for Integrated BioSystems, USU
• Lance Seefeldt, Ph.D., Chemistry and Biochemistry, USU
• Ron Sims, Ph.D., Biological and Irrigation Engineering, USU
• Tim Taylor, Ph.D., Biological and Irrigation Engineering, USU
Gene Expression and Microarray Analysis
June 22-24, 2010

This is an intensive training Day 1 Day 3

course covering the main areas
of microarray technology.
The course will focus on
microbial as well as plant gene
expression and will emphasize
theory, sample preparations,
labeling, fluorescent imaging,
microarray data analysis,
Lecture:
Overview of gene expression &
microarrays
Genome sequencing, oligonucleotide
arrays, labeling & sensitivity
Spotting media
Laboratory:
Printing arrays and quality
considerations
Pitfalls & troubleshooting of
common microarray problems
Lecture:
Statistics for microarray analysis
Mining of gene expression data base
Measuring error and data
transformation for gene expression
Laboratory:
Data analysis and modeling: image
& raw data, normalization methods
Bioconductor SAM, Array Assist,
Pathway Tools, NetAffy

and data mining. Hands- Day 4

on laboratory sessions will
cover microarray tools,
kits, reagents and services,
experimental design, labeling
cDNA synthesis, sample
preparation, microarray
reaction, microarray detection,
data analysis and modeling,
Affymetrix demonstration and
more.
Registration: $1650
Early registration: $1550
Special rates are available
for academic and
government attendees.
Day 2 Case Studies :
Lecture: Alfalfa, bacterial metabolomics with
Microbial gene expression arrays, yeast & fatty acid production
Eukaryotic gene expression
Sample quality before hybridization ~ Adjourn at noon
Laboratory:
AFFYMETRIX ARRAYS: Sample
prep, hybridization, array scanner,
image and raw data
Instructors:
• Patti Champine, M.S., Center for Integrated BioSystems, USU
• Daryll DeWald, Ph.D., Biology Department, USU
• Jacob Parnell, Ph.D., Center for Integrated BioSystems, USU
• Kamal Rashid, Ph.D., Center for Integrated BioSystems, USU
• Giovanni Rompato, Ph.D.., Center for Integrated BioSystems, USU
• John Stevens, Ph.D., Mathematics and Statistics, USU
International and Custom Programs
The Center for Integrated
BioSystems is engaged in
a number of international
training programs and
outreach activities in several
countries, including Dominican
Republic, Singapore, and
Thailand. Efforts are
underway to expand to China,
Vietnam, and India. The
programs range from capacity
building and manpower
development in developing
countries to improving and
expanding the existing
capabilities for biotechnology
in more developed countries.
The programs are tailored to
the needs of the host country.
Contact us for custom courses
that meet your needs.
Dr. Kamal Rashid
(435) 797-3504
kamal.rashid@usu.edu
Custom Courses
The Center for Integrated BioSystems
has the ability to design custom
courses to fit your organization’s
specific needs, taught either at our
facility or yours. Please contact our
program director, Dr. Kamal Rashid,
to discuss content needs, costs
and timing.
(435) 797-3504
kamal.rashid@usu.edu
6
Contact Us
We are available to answer questions
about our courses and to help make
your participation as effective and
convenient as possible. For additional
registration information, please call
us directly or visit our website at
www.biosystems.usu.edu/ training.
(435) 797-3504
ken.olsen@usu.edu
Dominican Republic
Biosafety and GMO testing
in Dominican Republic
Thailand
Nuclear transfer and stem cell research
techniques in Thailand
Singapore
Scale-up strategies for mammalian and
microbial cell bioprocessing
in Singapore
Additional Program Details
Acclaimed speakers from both industry and academia,
well-trained in areas of cell culture, fermentation,
separation, purification, and molecular biology techniques.
Small, friendly classes emphasizing hands-on training.
Integration of theory with practical laboratory exercises.
Lecture notes and lab protocols provided for future
reference.
30,000+ sq. ft. research center offering state-of-the- art
equipment for course use.

Location Accommodations and Ground Transportation

The training programs are held in the Center for Integrated
BioSystems at Utah State University. The 400-acre campus is
located in northern Utah’s beautiful city of Logan, nestled within
the Rocky Mountains approximately 1½ hours from the Salt Lake
City airport. The immediate area offers unbeatable recreational
activities, such as skiing, snowboarding, snowmobiling, boating,
cycling, hiking, hunting, fi shing and breathtaking sight-seeing.
Additional visitor information is available on our Web site at
www.biosystems.usu.edu/home/visitors.
The University Inn, at the heart of the USU campus, is
a moment’s walk from the Center and is popular for its
accessibility. A block of rooms at a discount price has been
reserved for training program participants. For reservations,
please call 1-800-231-5634. A list of additional hotels in the
area is available on our Web site and will be included in your
confi rmation packet. For shuttle services from Salt Lake City
Airport, please contact Cache Valley Airport Shuttle at 1-800-
658-8526.

Distances to Ski Areas and Attractions: Custom Courses

The Center for Integrated BioSystems has the ability to design
Bear Lake 30 miles
custom courses to fit your organization’s specific needs, taught
Salt Lake City: 82 miles
either at our facility or yours. Please contact our program
-Alta, Brighton, & Snowbird Ski Areas
director, Dr. Kamal Rashid, to discuss content needs, costs and
Park City: 116 miles
timing.
-Deer Valley, The Canyons, & Park City Ski Resort
Phone: (435) 797-2739
-Olympic Bobsled Park
Email: kamal.rashid@usu.edu
Jackson, Wyoming 186 miles
Arches National Park 309 miles Contact Us
Yellowstone National Park 311 miles We are available to answer questions about our courses and
Moab 313 miles to help make your participation as effective and convenient as
Canyonlands National Park 329 miles possible. For additional registration information, please visit
Lake Powell, Arizona 375 miles our Web site at www.biosystems.usu.edu/training, or contact us
Bryce Canyon National Park 403 miles directly at:
Zion National Park 440 miles
Grand Canyon Nat’l Park (North Rim) 465 miles Phone: (435) 797-3504
Grand Canyon Nat’l Park (South Rim) 592 miles
Las Vegas, Nevada 493 miles
Fax: (435) 797-2766
Email: ken.olsen@usu.edu

About the Program Director

Dr. Kamal A. Rashid has more than 20
years of academic experience in both
research and training development.
During his career, he has developed,
directed and implemented biotechnol-
ogy training at both Penn State and,
since 2000, at Utah State University.
His training programs have been inter-
nationally recognized and conducted
in Singapore, Egypt, the Dominican
Republic Korea, Malaysia, Taiwan,
Thailand, Philippines and Puerto Rico. Dr. Rashid’s longstand-
ing presence in academic and industrial circles makes USU’s
training program one of the leading programs of its kind.
Core Service Laboratories
We have multiple core laboratories
at the Center for Integrated
BioSystems. Each core laboratory
covers multiple technologies and
services.
Our core laboratory scientists and
technical staff are well trained and
have years of experience. They are
available to offer you accurate,
well-documented, and timely
services.
Contact us for services
that meet your needs.
Main office:
(435) 797-2753
or order online:
www.biosystems.usu.edu/core_
labs
Genomics Core
The CIB is involved in DNA sequencing,
genotyping, gene expression analysis,
and characterization of gene function.
The Genomics Group offers rapid
turn-around and delivery on sample
submission. Typically a DNA sample
can be received, prepared, and
sequenced in as little as 24 hours.
Proteomics Core
The Proteomics Group in the Center
for Integrated BioSystems is set
up to help researchers in novel
biomarker discovery, discovering
molecular models for disease, protein
identification from complex mixtures
with high coverage and global protein
expression profi ling, and protein
flux analysis with or without labeling
protein mixtures.
Metabolomics Core
The Metabolomics lab will add the
option of sample submission for
metabolic reconstruction and small
molecule analysis using LC/MS. We are
developing our service in this area and
would welcome discussion of your needs
for metabolic reconstruction and small
molecule analysis.
8
Fermentation Core
The Fermentation staff at the CIB is
available to partner with researchers
to take a process from concept to
realization quickly and efficiently.
We offer production services in
microbial fermentation and downstream
processing to clients in all fields.
Additionally, large scale trials capability
at the shake fl ask and bench-top
fermentor scale, backed up by the full
analytical capabilities of the core labs,
is available for optimizing parameters,
screening compounds and answering a
variety of other research questions.
Bioinformatics Core
The Bioinformatics service offers a
broad spectrum of analyses that are
suited for today’s biologists with high
throughput data. From single sequence
feature analysis to multigenome
comparisons to microarray data
analysis and visualization, a large set
of capabilities is available in-house for
rapid layering of a biological context
onto any data type.
Custom Projects
Our scientists often engage in custom
research projects for our clients.
Contact us if you need to outsource
a project.
How to Register
Participants can register by mailing or faxing this form to the Center for Inte-
grated BioSystems or by using our secure online registration form. To qualify for Updates!
the early registration rate, participant registration forms must be received by our If you would like to continue receiving
office more than 30 days in advance of the course start date. mailings about our training program –
regardless of course registration at this
Online: www.biosystems.usu.edu/register
time – please complete and return the
Fax: (435) 797-2766 Personal Information form at left. You
Mail: Utah State University Center for Integrated BioSystems can also register to receive our updates
on our Web site at: www.biosystems.
Biotechnology & Bioprocessing Training Program
usu. edu/trainingupdates.
4700 Old Main Hill, Logan UT 84322-4700

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*There is a cancellation fee of $100 until 14 days prior to the start of the program, after which there will be no refund. The university reserves the
right to cancel any course for lack of adequate participants. In such an event, a full refund will be provided to the participant.

Course Selection
Please indicate the course(s) for which you are registering. This
form may be duplicated for additional registrants.

Microbial Fermentation: Development and Scale-up Gene Expression and Microarray Analysis
$1750 ($1650 early registration) $1650 ($1550 early registration)
March 16-19, 2010 May 18-21, 2010 June 22-24, 2010
October 19-22, 2010
Downstream Processing of Biological Products
Techniques in Animal Cell Culture and Scale-up Strategies $1750 ($1650 early registration)
$1750 ($1650 early registration) September 14-17, 2010
April 13-16, 2010 November 16-19, 2010

Program Policies
Early Registration and
Tuition Discounts
Register more than 30 days before the fi rst day of the program
to take advantage of our discounted course fee. We will also
extend a 10% registration discount for organizations sending
more than one attendee. Please inquire about the availability
of academic and government employee discounts. The last day
to register is 14 days before the fi rst day of the course.
Fees and Cancellations
Fees include the course manual of instructions, refreshments,
daily continental breakfast, a welcome dinner, and a closing
banquet on the fi nal evening. There is a cancellation fee of
$100 until 14 days prior to the start of the program, after which
there will be no refund. We reserve the right to cancel any
course for lack of registrants. In such an event, a full refund
will be provided to the participant.
 In our 10th year of
leading the industry
in technical,
hands-on training

2010 Biotechnology
& Bioprocessing
Training Programs

4700 Old Main Hill. Logan Utah 84322-4700

www.biosystems.usu.edu/training 435 797 3504 Understanding the molecules of life