Professional Documents
Culture Documents
Operons
• Prokaryotes must use substances and synthesize macromolecules just fast enough to meet their
needs. If enzymes needed, gene transcribed. If enzyme not needed, gene turned off. Allows for
conservation of cell resources. Controlling gene expression is one method of regulating metabolism.
The other method of controlling metabolism involves (you
should know this!)
• Operon--Genes grouped together; transcribed into single mRNA molecule containing coding
sequences for more than one gene (polycistronic gene; “cistron”= gene). Grouped together with one
promoter.
• Operons can be inducible or repressible. Inducible products are made when the substrate is in the
environment (lactose) and needs to be metabolized. Repressible products are made when a signal
molecule is scarce (ie. Biosynthetic pathways such as tryptophan)
• Allows for coordinated control of genes required for metabolism. One switch controls more than
one gene.
• Not present in Eukaryotes. Eukaryotes have more complex methods of controlling gene expression.
(Next Chapter!)
Binding of repressor to operator prevents transcription – GENE OFF [RNA poly-ase not able to bind to
promoter].
Operators are regions of DNA (15 nucleotides long) that control RNA access to promoter. Without
repressors transcription will take place. Repressors are regulatory proteins that bind to operator and turn
genes off (negative control mechanism). [Acts as a braking mechanism] They are produced at a site
away from the operon by a specific regulatory gene.
Will occur if high levels of gene product (tryptophan) bind to activate repressor. Require some
threshold limit of product. Acts as corepressor (metabolite) by binding to repressor molecules which
then bind to operator and turn gene off.
Repressible Operons are usually turned on until metabolite (trp in this case) activates the repressor.
This would occur in a bacterium if it is in the guts of an organism that has just eaten and supplied
bacteria with tryptophan!
Repressible and Inducible enzymes are both an example of negative control of a pathway. Activating
the repressor proteins shuts off the pathway. Positive control requires that an activator molecule switch
on transcription.
Positive gene regulation occurs when a protein binds to another, thus enhancing the RNA poly-ase
binding to the promoter. This interacts with the genome to act as an [Ignition switch] to turn on
transcription. The presence of lactose alone is not enough to deactivate the repressor and cause
transcription of the operon. Glucose must also
be absent since a cell will preferentially
metabolize glucose over lactose.