Description of all Mathematics, Computational and

departmental courses taken during 7 semesters at IIT

Guwahati

 SEMESTER – 1  
Course Course Name L–T–P–C
No.
     
MA 101 Mathematics - I 3–1–0–8
CH 101 Chemistry 3–1–0–8
CH 110 Chemistry Laboratory  0–0–3–3
PH 101 Physics - I 2–1–0–6
ME 110 Workshop I 0–0–3–3
EC 101 Electrical Sciences
3–1–0–8
ME 111 Engineering Drawing - I 2–0–3–7
HS 110 Introduction to cultural studies 3–0–0–6

SEMESTER – 2  
Course Course Name L–T–P–C
No.
     
MA 102 Mathematics - II 3–1–0–8
ME 101 Engineering Mechanics 3–1–0–8
EC 102 Basic Electronics Laboratory 0–0–4–4
CS 101 Introduction to Computing 3–0–0–6
CS 110 Computing laboratory 0–0–3–3
PH 102 Physics - II 2–1–0–6
BT 101 Modern Biology 3–1–0–8
PH 110 Physics laboratory 0–0–3–3

SEMESTER – 3  
Course Course Name L–T–P–C
No.
     
MA 201 Mathematics III 3–1–0–8
HS 217 Traditional modernity and social 3–0–0–6
changes
CS 201 OOP and Data Structures 3–0–3–9
CL 201 Chemical Process Calculations 2–1–0–6
BT 205 Biophysics 2–1–0–6
BT 201 Biochemistry 3–0–0–6
BT 210 Biochemistry Laboratory
SEMESTER – 4  
Course Course Name L–T–P–C
No.
     
CH 220 Consumer chemistry 3–0–0–6
HS 216 Sociology of India 3–0–0–6
CL 203 Thermodynamics I 2–1–0–6
CL 205 Mass Transfer Operations I
2–1–0–6
BT 202 Microbiology 3–1–0–8
BT 220 Microbiology Laboratory 0–0–6–6
BT 208 Molecular Biology and Genetic
Engineering 3–1–0–8
BT 280 Molecular Biology and Genetic
Engineering Laboratory 0–0–6–6

SEMESTER – 5  

Course Course Name L–T–P–C

No.
     
CL 303 Chemical Reaction Engineering I 2–1–0–6
BT 309 Plant Biotechnology 3–0–0–6
BT 390 Plant Biotechnology Laboratory 0–0–6–6
BT 303 Immunology 3–0–0–6
BT 305 Industrial Microbiology 3–0–0–6
BT 307 Environmental Biotechnology 2–1–0–6
BT 308 Animal Cell Biotechnology 3–0–0–6

SEMESTER – 6  

Course Course Name L–T–P–C

No.
     
HS 314 Science, Technology & Innovation 3–0–0–6
BT 302 Biochemical Engineering 3–1–0–8
BT 320 Biochemical Engineering 0–0–6–6
Laboratory
BT 304 Bio-separation Engineering 3–0–0–6
BT 306 Bio-informatics and Computational 3–0–3–9
Biology
BT 300 Seminar 0–0–3–3

SEMESTER – 7  

Course Course Name L – T – P – C 

No.
     
NT 710 Nano-Biosciences 3–0–0–6
BT 620 Biosensors 3–0–0–6
BT 612 Systems Biology 3–0–0–6
BT 401 Frontiers in Biotechnology 3 – 0 – 0 – 6 
BT 405 Bio-safety, Ethics and IPR 2–0–0–4
(MA101) MATHEMATICS-I

Systems of linear equations and their solutions; vector space Rn and its
subspaces; spanning set and linear independence; matrices, inverse and
determinant; range space and rank, null space and nullity, eigenvalues
and eigenvectors; diagonalization of matrices; similarity; inner product,
Gram-Schmidt process; vector spaces (over the field of real and complex
numbers), linear transformations.

Convergence of sequences and series of real numbers; continuity of

functions; differentiability, Rolle's theorem, mean value theorem,
Taylor's theorem; power series; Riemann integration, fundamental
theorem of calculus, improper integrals; application to length, area,
volume and surface area of revolution.

Texts:
1. D. Poole, Linear Algebra: A Modern Introduction, 2nd
Edn.,Brooks/Cole, 2005.
2. G. B. Thomas, Jr. and R. L. Finney, Calculus and Analytic Geometry,
9th Edn., Pearson Education India, 1996..

References:
1. G. Strang, Linear Algebra and Its Applications, 4th Edn.
Brooks/Cole India, 2006.
2. K. Hoffman and R. Kunze, Linear Algebra, 2nd Edn., Prentice Hall
India, 2004.
3. R. G. Bartle and D. R. Sherbert, Introduction to Real Analysis, 3rd
Edn., Wiley India, 2005.
4. S. R. Ghorpade and B. V. Limaye, An Introduction to Calculus and
Real Analysis, Springer India, 2006.

(MA102) MATHEMATICS II
Vector functions of one variable - continuity and differentiability;
functions of several variables - continuity, partial derivatives,
directional derivatives, gradient, differentiability, chain rule; tangent
planes and normals, maxima and minima, Lagrange multiplier
method; repeated and multiple integrals with applications to volume,
surface area, moments of inertia, change of variables; vector fields,
line and surface integrals; Green's, Gauss' and Stokes' theorems and
their applications.
First order differential equations - exact differential equations,
integrating factors, Bernoulli equations, existence and uniqueness
theorem, applications; higher-order linear differential equations -
solutions of homogeneous and nonhomogeneous equations, method
of variation of parameters, operator method; series solutions of
linear differential equations, Legendre equation and Legendre
polynomials, Bessel equation and Bessel functions of first and second
kinds; systems of first-order equations, phase plane, critical points,
stability.

Texts:
1. G. B. Thomas, Jr. and R. L. Finney, Calculus and Analytic
Geometry, 9th Edn., Pearson Education India, 1996.
2. S. L. Ross, Differential Equations, 3rd Edn., Wiley India, 1984.

References:
1. T. M. Apostol, Calculus - Vol.2, 2nd Edn., Wiley India, 2003.
2. W. E. Boyce and R. C. DiPrima, Elementary Differential
Equations and Boundary Value Problems, 9th Edn., Wiley India,
2009.
3. E. A. Coddington, An Introduction to Ordinary Differential
Equations, Prentice Hall India, 1995.
4. E. L. Ince, Ordinary Differential Equations, Dover Publications,
1958.
(MA201) MATHEMATICS-III

Complex Analysis: Analytic functions. Cauchy-Riemann equations,

Laplace equations. Elementary functions. Cauchy's integral theorem,
Cauchy's integral formula. Taylor series and Laurent series. Residues
and its applications to evaluating real integrals. Conformal mappings.
Partial Differential Equations: First and second order partial
differential equations. Classification of second order equations.
Cauchy problems. Dirichlet and Neumann boundary value problems.
Boundary value problems involving wave equation, the heat
equation, the Laplace equation. Solutions by the method of
separation of variables.
Laplace and Fourier Transforms: Laplace transform. Inverse
transform. Shifting on the s and t axes, convolutions, partial fractions.
Fourier series and Fourier transforms. Solutions of ordinary as well
as partial differential equations by Laplace and Fourier transforms.

Texts:
1. R. V. Churchill and J. W. Brown, Complex Variables and
Applications, 5th Edition, McGraw-Hill, 1990.
2. J. H. Mathews and R. W. Howell, Complex Analysis for
Mathematics and Engineering, 3rd Edition, Narosa, 1998.
3. I. N. Sneddon, Elements of Partial Differential Equations,
McGraw-Hill, 1957.
4. K. Sankara Rao, Introduction to Partial Differential Equations,
Prentice Hall of India, 1995.
5. K. Sankara Rao, Introduction to Partial Differential Equations,
Prentice Hall of India, 1995.
6. E. Kreyszig, Advanced Engineering Mathematics, 5th / 8th
Edition, Wiley Eastern/ John Wiley, 1983 / 1999.

References:
1. E. Kreyszig, Advanced Engineering Mathematics, 5th / 8th
Edition, Wiley Eastern / John Wiley, 1983/1999.
(BT101) MODERN BIOLOGY          

Diversity in biological systems; cell biology and cell structure;

biological membranes; bioenergetics; genetics: DNA as genetic
material; structure of DNA; DNA replication; transcription;
translation; genes to proteins and to protein function; gene
expression and regulation; recombinant DNA technology. Human
physiology: biological axons and neurons, neuromuscular and
synaptic junctions; sensory systems - hearing, taste, smell and visual
receptors. 

Texts: 
1. J. L. Tymoczko, J. M. Berg and L. Stryer, Biochemistry, 5 th Ed, W.
H. Freeman & Co, 2002.
2. D. L. Nelson and M. M. Cox, Lehninger Principles of
Biochemistry, Macmillan Worth, 2000.  

References: 
1. N. Hopkins, J. W. Roberts, J. A. Steitz, J. Watson and A. M.
Weiner, Molecular Biology of the Gene, 4th Ed, Benjamin
Cummings, 1987.
2. C. R. Cantor and P. R. Schimmel, Biophysical Chemistry (Parts I,
II and III), W.H. Freeman & Co., 1980.
3. C. C. Chatterjee, Human Physiology, Vol 1 & 2, 11th Ed, Medical
Allied Agency, 1987.
(BT205) BIOPHYSICS 

Structure and structural dynamics of DNA, RNA and proteins;

techniques for monitoring structure and dynamics: absorption,
fluorescence, circular dichroism, light Scatterin; methods for
separation and characterization of molecules; size-exclusion
chromatography; electrophoresis; MALDI–TOF; ESI–quadrupole
mass spectrometry; thermodynamics and equilibria of
macromolecules in solution; biophysics of membranes;
photobiological processes; nerve impulse; muscle contraction;
modelling biological processes; protein folding and aggregation;
protein function (Myosin and Kinesin). 

Texts: 
1. K. E. van Holde, W. C. Johnson and P. S. Ho, Principles of
Physical Biochemistry, Prentice Hall, 1998.
2. C. R. Cantor and P. R. Schimmel, Biophysical Chemistry (Parts I,
II and III), W. H. Freeman and Co., 1980. 

Reference: 
1. K. A. Dill and S. Bromberg, Molecular Driving Forces. Statistical
thermodynamics in Chemistry and Biology, Garland Science,
2003.
2. B. Nolting, Methods in Modern Biophysics, Springer Verlag,
2004.
(BT201) BIOCHEMISTRY   

Basic concept and design of metabolism; carbohydrate metabolism:

glycolysis, gluconeogenesis, citric acid cycle, pentose phosphate
pathway, glycogen metabolism, oxidative phosphorylation;
photosynthesis; fatty acid metabolism; protein: synthesis, targeting
and turnover; biosynthesis of amino acids and nucleotides;
Integration of metabolisms; hormones; enzymes: structure,
mechanism and reaction kinetics; introduction to information
metabolism. 

Texts: 
1. D. L. Nelson and M. M. Cox, Lehninger Principles of
Biochemistry, Macmillan Worth, 2000.
2. J. L. Tymoczko, J. M. Berg and L. Stryer, Biochemistry, 5 th Ed, W.
H. Freeman, 2002. 

References: 
1. W. W. Parson, D. E. Vance and G. L. Zubay, Principles of
Biochemistry, Wm. C. Brown Publishers, 1995.
2. K. E. Van Holde, C. K. Mathews and K. G. Ahern, Biochemistry,
Pearson Education, 2000.
3. R. K. Murray, D. K. Granner, P. A. Mayes and V. W. Rodwell,
Harper’s Biochemistry, McGraw Hill, 2002.
(BT202) MICROBIOLOGY         
Microbial cell structure and function; fundamentals of microbial
taxonomy and diversity; molecular tools in microbial taxonomy;
microscopic techniques; microbial nutrition; growth and control;
microbial metabolism; mutations and DNA repair; plasmids;
transformation; conjugation; transduction; transposons;
fundamentals of gene regulation; fundamentals of microbial
genomics; microbial pathogenicity and diseases. 

Texts: 
1. G. Tortora, B. Funke and C. Case, Microbiology, An Introduction
(International Edition), 8th Ed, Pearson Education, 2003.
2. M. Madigan, J. Martinko and J. Parker, Brock Biology of
Microorganisms, 10th Ed, Prentice Hall, 2002. 

References: 
1. R. Y. Stanier, J. L. Ingraham, M.L. Wheelis and P. R. Painter,
General Microbiology, 5th Ed, Macmillan Press, 1987.
2. 2L. M. Prescott, J. P. Harley and D. A. Klein, Microbiology, 6 th Ed,
McGraw Hill, 2005.
3. J. G. Black, Microbiology: Principles & Explorations. 5 th Ed, John
Wiley & Sons Inc., 2002.
4. Benjamin Lewin, Genes VIII (International Edition), Pearson
Education, 2004.  
(BT208) MOLECULAR BIOLOGY & GENETIC
ENGINEERING   

Cell organization and subcellular structure; structure and properties

of nucleic acids; organization of prokaryotic and eukaryotic genomes;
mechanisms of DNA replication; mutagenesis and processes of DNA
repair; transcription; translation; mechanisms of DNA
recombination; regulation of gene expression; eukaryotic RNA
splicing and processing; cell cycle; programmed cell death; cell
transformation; genes in differentiation and development;
oncogenes. genetic engineering: restriction modification enzymes;
cloning vectors: plasmids, phages, cosmids, phagemids, yeast and
bacterial artificial chromosomal vectors; construction cDNA and
genomic libraries; screening of libraries: by DNA hybridization,
immuno and protein assays; gene cloning and expression in
prokaryotes and eukaryotes; recombinant protein expression in E.
coli, yeast and baculovirus; mammalian cell expression vectors
(Selectable markers, Two-hybrid expression system); chimeric
vectors; Site-directed mutagenesis and its applications; transposons,
gene targeting; site specific recombination; polymerase chain
reaction (PCR); applications of reverse transcription PCR (RT-PCR)
and real time PCR; principles and applications of DNA finger printing;
gene mapping by restriction fragment length polymorphism (RFLP);
application of differential display and subtractive hybridization. 

Texts:          
1. B. Alberts, A. Johnson, J. Lewis, M. Raff, K. Roberts and P.
Walter, Molecular Biology of Cell, 4th Ed, Garland Publishing,
2002.
2. H. Lodish, A. Berk, S. L. Zipursky, M. P. Scott and J. Darnell,
Molecular Cell Biology, 4th Ed, W. H. Freeman & Co.,
2003.                                         
3. B. Lewin, Genes VIII, International Edition, Pearson education,
2004. 

Reference: 
1. B. R. Glick and J. J. Pasternak, Molecular Biotechnology:
Principles and Applications of Recombinant DNA, 3rd Ed, ASM
Press, 2003.
2. R. M. Twyman, S. B. Primrose and R. W. Old, Principles of Gene
Manipulation, Blackwell Science, 2001.
(BT309) PLANT BIOTECHNOLOGY  

Plant morphogenesis; cellular totipotency; in vitro culture; protoplast

isolation and culture; somatic hybridization; haploid and triploid
production; somaclonal variation; embryo rescue and synthetic
seeds; production of secondary metabolites; cryopreservation and
conservation of germplasm; plant gene structure, function and
regulation; plant transformation; marker genes; promoters;
molecular analysis; chloroplast transformation; genetic engineering
for resistance to insects, pests, virus, pathogens and tolerance to
herbicides; gene silencing; metabolic engineering; molecular farming;
molecular markers for plant improvement; plant genomics. 

Texts:  
1. A. Slater, N. Scott, M. Fowler, Plant Biotechnology: The genetic
manipulation of plants, Oxford University Press, 2003.
2. S. S. Bhojwani and M. K. Razdan, Plant Tissue Culture: Theory
and Pratice, Elsevier, 1996.
3. R. Smith, Plant Tissue Culture: Techniques and Experiments.
2nd Ed, Academic Press, 2000. 

References: 
1. J. Hammond, P. McGarvey and V. Yusibov, Plant Biotechnology:
New Products and    Applications, Springer Verlag, 1999.
2. P. Jones, P. J. Jones and J. M. Sutton, Plant Molecular Biology:
Essential Techniques, John Wiley & Sons. 1997.
3. I. Potrykus and G. Spangenberg, Gene Transfer to Plants,
Springer Verlag, 1995.

(BT303) IMMUNOLOGY     
Introduction to immune system; evolution of immunity; elements of
Immune system; cell migration & inflammation; immunogens &
antigens; antibody structure and function – catalytic Antibodies,
antigen antibody interactions, humoral and cellular immunity – MHC,
antibody diversity, dendritic cells (APC), control mechanisms in the
immune response cytokines – complement – autoimmunity;
immunity to different types of pathogens, vaccination; tumor
immunology; immune diseases and disorders; transplantation
immunology; co-stimulatory pathways; hybridoma and
immunoassays. 

Texts: 
1. I. Roitt, J. Brostoff and D. Male, Immunology, 6th Ed, Harcourt
Publishers, 2001.
2. R. A. Goldsby, T. J. Kindt, B. A. Osborne and J. Kuby,
Immunology, W. H. Freeman & Co, 2003.
3. A. K. Abbas, A. H. Lichtman and J. S. Pober, Cellular and
Molecular Immunology, W. B. Saunders Co., 2000.  

Reference: 
1. D. M. Weir and J. Stewart, Immunology, Churchill Livingstone,
1997.
2. Cooke, M. Owen, J. Trowsdale, B. Champion and D. K. Male,
Advanced Immunology, Mosby Publ., 1996.
3. R. Coico, G. Sunshine and E. Benjammini, Immunology: A short
Course, Wiley-Liss Publ., 2003.

(BT305) INDUSTRIAL MICROBIOLOGY       
     Basis and development of Industrial Fermentation Processes
(Screening cultures, media, inoculum, scale-up); Control of
microorganisms; Fermentation Processes; Microbial production of
Antibiotics, Alcohols, Enzymes, Organic acids, Amino acids, Vitamins,
Biopolymers; Microbial polysaccharides; Bioplastics; Biosurfactants;
Bioinsecticides; Pigments and flavors with their applications;
Microbial leaching; Bio-transformation; Bio-degradation; Food
production involving microorganisms and their products;
Fermentation involving genetically engineered microbes; Industrial
applications of extremophiles; Safety aspects of industrial processes. 

Texts: 
1. M. A. Malden, Industrial Microbiology: An Introduction,
Blackwell Science, 2001. 
2. Alexander N. Glazer and Hiroshi Nikaido, Microbial
Biotechnology: Fundamentals of Applied Microbiology, W. H.
Freeman & Co, 1995.  

References:  
1. L. Demain, R. M. Atlas, G. Cohen, C. L. Hershberger, W. S. Hu, D.
H. Sherman, R. C. Wilson and J. H. David, Manual of Industrial
Microbiology and Biotechnology, 2nd Ed, ASM Press, 1999.
2. H. J. Rehm and G. Reed, Biotechnology: A Comprehensive
Treatise, VCH publisher, 2001.

(BT307) ENVIRONMENTAL BIOTECHNOLOGY       

Introduction to environment; pollution and its control; pollution
indicators; waste management: domestic, industrial, solid and
hazardous wastes; strain improvement; biodiversity and its
conservation; microbes for bioremediation technology such as
petroleum, hydrocarbon decontamination, radionuclei, toxic metal,
dyes and lignin removal and xenobiotics; phytoremediation; biomass
for removal and biosorption of heavy metal and other inorganic ions;
removal of volatile organic compounds from waste gas. 

Texts: 
1. B. Ritmann and P. L. McCarty, Environmental Biotechnology:
Principle & Applications, 2nd Ed, McGraw Hill Science, 2000.
2. G. M. Evans and J. C. Furlong, Environmental Biotechnology:
Theory and Applications, Wiley Publishers, 2002. 

References: 
1. H. S. Peavy, D. R. Rowe and G. Tchobanoglous, Environmental
Engineering, McGraw-Hill Inc., 1985.
2. J. S. Devinny, M. A. Deshusses and T. S. Webster, Biofiltration
for Air Pollution Control, CRC Press, 1998.
3. H. J. Rehm and G. Reed, Biotechnology – A Multi-volume
Comprehensive Treatise, 2nd Ed, Vol 11, VCH Publishers Inc.,
1993.

(BT308) Animal Cell BIOTECHNOLOGY

Animal cell biotechnology: Animal cell and tissue engineering; Animal
cell culture techniques relevant to mRNA knockdown (e.g. antisense
and ribozyme technology); generation of immortalized cell lines. In
vitro organogenesis; Stem Cells; Differentiation of animal and human
cells; Animal cloning; Mechanisms of drug resistance and cell death;
Basic developmental Biology; Structure and organization of tissues;
Cell Surface markers; FACS analysis; Cell Migration: control of cell
migration in tissue engineering; Transplantation immunology. 

Texts: 
1. T. A. Brown, Gene Cloning and DNA Analysis: An Introduction,
Blackwell Science, 2001. 
2. T. A. Brown, Genomes, 2nd Ed, BIOS Scientific Publishers, 2002.
3. B. R. Glick and J. J. Pasternak, Molecular Biotechnology:
Principles and Applications of Recombinant DNA, 3rd Ed, ASM
Press, 2003. 

Reference: 
1. R. I. Freshney, Animal Cell Culture:  A Practical Approach, 2nd
Ed, IRL Press, 1992.
2. R. E. Spier, Encyclopedia of Cell Technology, Vols 1 & 2, Wiley
Biotechnology Encyclopedia, John Wiley & Sons, 2000.
3. A. Doyle, J. B. Griffiths and D. G. Newell, Cell and Tissue Culture
Laboratory Procedures, John Wiley & Sons, 1998.

(BT302) BIOCHEMICAL ENGINEERING       

Unique features of the field, definitions and concepts, introduction of

organisms as mass and energy consumers, metabolic stoichiometry
and energetics, kinetics of substrate utilization, product formation
and biomass production in cell cultures, Transport phenomena in
bioprocess systems: gas-liquid mass transfer in cellular systems,
determination of oxygen transfer rates, Design and analysis of
biological reactors: batch, continuous, fed-batch, fluidized bed
reactor, packed bed reactor, bubble column, trickle bed reactor, 
animal and plant cell reactor technology, aeration and agitation,
Instrumentation and control: physical, chemical and biosensors,
online sensors, computers and interfaces,  scale up, applied enzyme
catalysis; media and air sterilization, product recovery, bioprocess
economics.  

Texts:  
1. H. W. Blanch and D. S. Clark., Biochemical Engineering, Marcel,
Dekker Inc., 1996.
2. J. E. Bailey and D. F. Ollis, Biochemical Engineering
Fundamentals, 2nd Ed, McGraw-Hill Inc., 1986.
3. P. A. Belter, E. L. Cussler and W. S. Hu, Bioseparations:
Downstream Processing for Biotechnology, John Wiley & Sons,
1988. 

References: 
1. H. J. Rehm  and G. Reed,  Biotechnology-A multi- Volume
Comprehensive Treatise, 2nd Ed, Vol 3, VCH, 1993
2. M. Moo-Young, Comprehensive Biotechnology, Vol 2, Pergamon
Press, 2004
3. S. Aiba, A. E. Humphrey and N. Millis, Biochemical Engineering,
Prentice-Hall 1978
4. P. F. Stanbury and A. Whitaker, Principles of fermentation
technology, Pergamon press , 1984
5. H. C. Vogel and C. L. Tadaro, Fermentation and Biochemical
Engineering Handbook - Principles, Process Design, and
Equipment, 2nd Ed, William Andrew Publishing/Noyes, 1997.
 

(BT304) BIOSEPARATION  ENGINEERING        

Down stream processing of products: filtration, centrifugation,

sedimentation, solvent extraction, aqueous two phase system,
sorption, precipitation, chromatography (ion exchange, size
exclusion, affinity, adsorption, hydrophobic interaction
chromatography, TLC, GLC, HPLC); membrane separations: reverse
osmosis, ultrafiltration; whole broth processing; Cell separation:
disruption by presses, homogenizers, milling, sonication, and non
mechanical methods; preparative electrophoresis; spectroscopic
techniques: UV-VIS, IR, NMR, mass, fluorescence, atomic adsorption,
flow cytometry; stabilisation of target product during bioseparation;
recent advances in reverse micellar techniques for bioseparation;
product recovery schemes for antibiotics, commercial enzymes and
organic acids. 

Texts: 
1. T. Nagamune, S. Katoh and T. Yonemoto, Bioseparation
Engineering, Elsevier Science Publication, 2002.
2. M. R. Ladisch, Bioseparations Engineering: Principles, Practice
and Economics, Wiley-Inter science 2001. 

References: 
1. R. G. Harrison, P. Todd, S. Rudge and D. P. Petrides,
Biosperations Sceince and Engineering, Oxford University
Press, 2003.
2. P. A. Belter, E. L. Cussler and W. S. Hu, Bioseparations:
Downstream Processing for Biotechnology, John Wiley & Sons,
1988.
 

(BT306) BIOINFORMATICS AND COMPUTATIONAL

BIOLOGY 

Introduction to bioinformatics; Gene bank sequence database;

submitting sequences to database; Analysis of genome content and
organization; Analysis of protein content and organization; Analysis
of protein structures; Identification of signature motifs in proteins;
Secondary structure prediction; Comparative genomics and
proteomics, Basics of aligning nucleic acid and protein sequences;
Phylogenetic analysis using internet; protein structure-function
relationships; computational analysis of protein-ligand binding;
enzyme catalysis and protein folding.  

Texts: 
1. D. Baxevanis, B. F. F. Ouellette, Bioinformatics – A practical
Guide to the analysis of Genes and Proteins, 2 nd Ed, John Wiley
and Sons Inc., 2001.
2. A. R. Leach, Molecular Modelling: Principles and Applications,
Addison-Wesley Pub. Co. 1997. 

Reference: 
1. P. E. Bourne and H. Weissig, Structural Bioinformatics, WILEY,
2003.
2. T. Lengauer; Bioinformatics - From Genomes to Drugs, Vols 1 &
2, Wiley-VCH, 2002.
 

(BT401) FRONTIERS IN BIOTECHNOLOGY            

Transgenic animals; gene knock in/out; phage display; shuttle

vectors; gene therapy; protein misfolding and associated disorders;
DNA and peptide vaccines; gene targeting; molecular markers; edible
vaccines; viruses in biotechnology; microarray; DNA chips and in
vitro evolution; functional genomics; systems biology.
 
Texts:
1. C. Ratledge and B. Kristiansen, Basic Biotechnology, 2nd ed,
Cambridge University Press, 2001.
2. D. Bourgagaize, T.R. Jewell and R. G. Buiser, Biotechnology:
Demystifying the Concepts, Wesley Longman, USA, 2000.
 
References:
1. P.N. Cheremisinoff, R.P. Ouellette and R.M. Bartholomew,
Biotechnology Applications and Research, Technomic Publishing
Co. Inc., USA, 1985.
2. D. Balasubramaniam, C.F.A. Bryce, K. Dharmalingam, J. Green,
and K. Jayaraman, Concepts in Biotechnology, Orient Longman
Pvt. Ltd., 2002.
3. S. Maulik and S.D. Patel, Molecular Biotechnology: Therapeutic
Applications & Strategies, Wiley-Liss; 1996.
4. Current opinions in Biotechnology (Journal), Elsevier Science.

(BT405) BIOSAFETY, ETHICS AND IPR    

Introduction to biosafety; classification and description of biosafety

levels; design of clean rooms and biosafety labs; biosafety regulations
to protect nature; growers and consumers interest and nation
interest; risk for animal/human/agriculture owing to genetically
modified organisms (GMOs); risk for environment owing to GMOs,
introduction and need for ethics; ethical issues involving GMOs;
ethical issues in India and abroad; introduction and the need for
intellectual property right (IPR); IPR in India and abroad.

Texts:
1. T. M Murray and M.J. Mehlman, Encyclopedia of Ethical, Legal
and Policy issues in Biotechnology, John Wiley & Sons 2000.

References:
1. P.N. Cheremisinoff, R.P. Ouellette and R.M. Bartholomew,
Biotechnology Applications and Research, Technomic Publishing
Co., Inc. USA, 1985.
2. D. Balasubramaniam, C.F.A. Bryce, K. Dharmalingam, J. Green
and K. Jayaraman, Concepts in Biotechnology, University Press
(Orient Longman Ltd.), 2002.
3. Bourgagaize, Jewell and Buiser, Biotechnology: Demystifying the
Concepts, Wesley Longman, USA, 2000.

(BT210) BIOCHEMISTRY LABORATORY    

      

Estimation of protein, carbohydrate and nucleic acid; enzymes:

isolation, purification and kinetics; electrophoresis of protein (SDS-
PAGE and Native-PAGE); western blotting; determination of melting
temperature (Tm) of DNA; protein unfolding; proton transport across
membrane; protein structure using structural coordinates procured
from protein data bank.  

Texts: 
1. R. Boyer, Modern Experimental Biochemistry, 3 rd Ed, Pearson
education (Singapore) Pvt. Ltd., 2001.
2. K.  Wilson and J. Walker (Ed), Practical Biochemistry, Principles
and Techniques, Cambridge University Press, 1995.
3. R. L. Switzer and L. F. Garrity, Experimental Biochemistry, 3 rd
Ed, W. H. Freeman, 1999.

(BT280) MOLECULAR BIOLOGY & GENETIC

ENGINEERING LABORATORY

Plasmid and genomic DNA isolation from E. coli, RNA isolation,

Restriction digestion and restriction fragment length polymorphism
analysis; PCR amplification; reverse transcription PCR analysis;
Purification of PCR product; Cloning of PCR product in T vectors;
preparation of competent cells; transformation and screening of
recombinant clones; b- gal expression; Southern blotting. 

Texts: 
1. J. Sambrook, D. W. Russell and J. Sambrook, Molecular Cloning,
A laboratory Manual, Cold Spring Harbor Laboratory, USA,
1999.  

Reference: 
1. D. M. Glover and B. D Hames, DNA Cloning II, IRL Press, 1995.
2. J. G. Chirikjian, Biotechnology: Theory and Practices (Genetic
Engineering, Mutagenesis and Separation Technology), Vol 2,
Jones & Bartlett Publishers, 1995.       
 

(BT220) MICROBIOLOGY LABORATORY     

Basic laboratory techniques for isolation, cultivation and cultural

characterization of microorganisms; microscopic techniques;
nutritional and physico-chemical requirements; enumeration of
microbial populations; biochemical activities of microorganisms;
cultivation and morphology of molds; microbiology of food, water
and soil.  

Texts: 
1. H. J. Benson, Microbiological Applications. Laboratory Manual in
General Microbiology, 8th Ed, McGraw Hill, 2002.
2. T. Johnson and C. Case, Laboratory Experiments in Microbiology,
7th Ed, Pearson Education, 2003.

(BT390) PLANT BIOTECHNOLOGY LABORATORY   

Preparation of plant tissue culture media; techniques of in vitro
culture; Agrobacterium-mediated transformation and transient GUS
expression studies in tobacco; biolistic gene transfer; molecular
analysis of transgenic plants; cloning of desirable gene in plant
binary vector; Agrobacterium transformation by tri-parental mating;
confirmation of transconjugants. 

Texts: 
1. J. H. Dodds and L. W. Roberts, Experiments in Plant Tissue
Culture, 3rd Ed, Cambridge University Press, 1995.
 2. S. J. Karcher, Molecular Biology: A Project Approach, Academic
Press, 2001.  

Reference: 
1. J. G. Chirikjian, Biotechnology: Theory and Techniques (Genetic
Engineering, Mutagenesis and Separation Technology), Jones &
Bartlett Publishers, UK. 1995.
2. H. Jones and John M.  Walker, Plant Gene Transfer and
Expression Protocols: Methods in Molecular Biology, 49,
Humana Press, 1996.
3. J. G. Chirikjian, Biotechnology: Theory and Techniques (Plant
Biotechnology, Animal Cell Culture and
Immunobiotechnology), Jones & Bartlett Publishers, UK. 1996.

(BT320) BIOCHEMICAL ENGINEERING   LABORATORY  

Media sterilization, inoculum preparation and scale up, control of

fermentation processes, batch reactors, measuring microbial growth
and biomass: growth kinetics, aeration principles, agitation and
power consumption in fermenters, bioreactors for fermentation and
bioconversion, scale up, continuous stirred tank bioreactors,
rheology; gas-liquid mass transfer-measurement of transfer
coefficients.

Texts: 
 1. J. E. Bailey and D. F. Ollis, Biochemical Engineering
Fundamentals, 2nd Ed, McGraw-Hill, Inc., 1986.
2. P. A. Belter, E. L. Cussler and W. S. Hu., Bioseparations:
Downstream Processing for Biotechnology, John Wiley & Sons,
1988. 

References: 
1. H. J. Rehm  and G. Reed., Biotechnology-A multi- Volume
Comprehensive Treatise, 2nd Ed, Vol 3, VCH, 1993.
2. M. Moo-Young, Comprehensive Biotechnology, Vol 2, Pergamon
Press, 2004.
3. P. F. Stanbury and A. Whitaker, Principles of fermentation
technology, Pergamon press, 1984.
4. S. Aiba, A. E. Humphrey and N. Millis, Biochemical Engineering,
Prentice-Hall 1978.