BAS 121 Biochemistry 2- 0- 2 = 3

Theory
Unit 1:
Enzymes: Nomenclature and classification, co-enzymes and co-factors, reaction and derivation of Michaelis-Menten equation,
inhibition kinetics and allosteric regulation of enzymes, isozymes, mode of catalysis.
Unit 2:
Carbohydrates: Definition classification, Basic structure, properties and functions of monosaccharides and related compounds, di-
saccharides and poly-saccacharides. Structural polysaccharides (cellulose and chitin). Storage polysaccharides (starch and glycogen).
Glycolysis, Kreb’s cycle, Pentose phosphate pathway, glycogenesis andglycogenolysis, gluconeogenesis. Electron Transport Chain,
Oxidative phosphorylation.
Unit 3:
Amino acids and Proteins: Structure, properties, classification and functions of amino acids, structure and function of proteins. Protein
denaturation and renaturation, proteins purification procedures. Transamination and deamination, decarboxylation and oxidative
degradation of amino acids, Nitrogen excretion and urea cycle.
Unit 4:
Lipids: Classification of lipids and fatty acids. General Structure and function of major lipid classes, Biosynthesis of odd and even
carbon saturated and unsaturated fatty acids, formation of ketone bodies, biosynthesis of triacylglycerols, membrane phospholipids,
cholesterol and steroids.
Unit 5:
Nucleic acid Structure and functions: Structure and properties of purine and pyrimidine bases.
Nucleic acid metabolism: Biosynthesis and break down of purine & pyrimidine nucleotide by de-novo and salvage pathway.
Practical
1. Preparation of buffers and determination of pH by pH meter and pH indicators.
2. Separation of sugar by ascending paper chromatography
3. Separation of amino acids by ascending paper chromatography
4. Isolation of proteins by size-exclusion chromatography
5. Separation of lipids by thin layer chromatography
6. Determination of reducing sugar by 3, 5- di-nitro salicylic Acid (DNSA) method.
7. Separation of proteins by SDS-PAGE
8. Determination the protein content of a given sample by Biuret method.
9. Titrimetric determination of lipase activity in oil
10. Estimation of DNA by Diphenylamine method
Textbooks
1. Lehninger, Nelson & Cox, Principle of Biochemistry, CBS Publication
2. Lubert stryer, Biochemistry, Freeman & Co, N.Y.
3. Voet & Voet, Fundamentals of Biochemistry, Jonh Willey & Sons
4. Harper’s Biochemistry, 25th edition, by R.K. Murray, P.A Hayes, D.K. Granner, P.A. Mayes and V.W. Rodwell (2000).
Prentice Hall International
5. Biochemistry, 4th edition, by G. Zubay (1998). Wm.C. Brown Publishers.
6. Biochemistry, 2nd edition, by Laurence A. Moran, K.G. Scrimgeour, H.R. Horton, R.S.Ochs and J. David Rawn (1994), Neil
Patterson Publishers Prentice Hall.
Course Outcome:
Students will be able to explain/describe the structure and general of proteins, lipids, nucleic acids, and carbohydrates and their role in
metabolic pathways along with their regulation through different mechanism of enzyme action.