Professional Documents
Culture Documents
net/publication/260511783
Article in Proceedings of the National Academy of Sciences, India - Section B: Biological Sciences · January 2015
DOI: 10.1007/s40011-014-0330-y
CITATIONS READS
2 1,011
9 authors, including:
Some of the authors of this publication are also working on these related projects:
Comperative assessment of immediate and heritable effect of nanoparticles and conventional mutagen. View project
All content following this page was uploaded by Aninda Mandal on 01 April 2015.
RESEARCH ARTICLE
Received: 8 November 2013 / Revised: 3 January 2014 / Accepted: 12 February 2014 / Published online: 1 March 2014
Ó The National Academy of Sciences, India 2014
Abstract Sixteen wild edible mushrooms collected from histopathological studies of heart, liver, lung and kidney
tropical dry deciduous forest of Eastern Chota Nagpur indicate safe consumption of the mushrooms. A simple and
Plateau, West Bengal, India are characterised morpholog- cost effective in vitro cultivation protocol of Volvariella
ically up to generic level considering fruit body and spore. volvacea has been developed. Preservation of mushrooms
Nutritional assessment (protein, fat, carbohydrate and ele- following salt, mustard oil and sun drying are found to be
ment contents) of fruiting bodies revealed their utility as effective. Present study suggests significant value of wild
nutritious food supplements. Results of food habit survey mushrooms towards domestication and commercialisation.
suggest that mushroom consumption (18.0 % of total food
items) is restricted only in mushroom growing period Keywords Economic benefits Food value
(June–October). Total of 8 mushrooms (Amanita sp. 2, Tropical dry deciduous forest Wild edible mushrooms
Astraeus sp., Termitomyces sp. 1–4, Volvariella sp. and
Lentinus sp.; price range Rs. 40–200/kg) are sold in local
markets. Sub-chronic toxicity test was conducted for Ter- Introduction
mitomyces sp. 2 and Lentinus sp. on Wistar rats at different
dosing (0, 1000, 2000 and 4000 mg/kg). Assessment of Edible mushrooms are fleshy fruit bodies of different
biochemical and hematological parameters and species of macrofungi, which are either hypogenous or
epigenous and can be picked up by hands. Edible mush-
rooms are used worldwide as supplementary food pos-
S. K. Das A. Mandal A. K. Datta (&) D. Das S. Halder sessing immense nutritive values. They are also well
Cytogenetics, Genetics and Plant Breeding Section, known for their medicinal applications [1–3] including
Department of Botany, Kalyani University, Kalyani 741235,
uses as anticancer, anti-diabetic, immune enhancing and
West Bengal, India
e-mail: dattaanimesh@gmail.com hypolipidemic properties [4]. Mushrooms are great recy-
clers and decomposers [5] and therefore play a significant
S. Gupta role in the ecosystem.
Pteridology-Palaeobotany Section, Department of Botany,
Edible mushrooms are economic mainstay of tribal
Kalyani University, Kalyani 741235, West Bengal, India
people and are essential forest products [6]. An edibility
R. Paul criterion of a mushroom encompasses absence of poison-
Department of Botany, Charuchandra College, ous effects on human and desirable taste and aroma [7].
Kolkata 700029, West Bengal, India
This requires proper identification knowledge to ensure
A. Saha safe edibility. It is well known that poisonous fungi are
Department of Botany, Narasinha Dutt College, frequently confused with edible mushrooms thus are
Howrah 711101, West Bengal, India responsible for mushroom poisoning which lead to fatal
sufferings [6]. In the Sal forest of Eastern Chota Nagpur
S. Sengupta
P.G. Department of Botany, Hooghly Mohsin College, Plateau of West Bengal, India, mushroom collection and
Hooghly 712101, West Bengal, India selling is an alternative source of income of the study
123
Author's personal copy
220 S. K. Das et al.
region. The tribal women (predominantly Santal and Lo- belong to red lateritic zone possessing fine to coarse loamy
dha) collect the naturally growing mushrooms, commonly soil dominated by Santal and Lodha. Mushroom collections
known as chhatu, from the forest and sell them to local were made during rainy (June–July) and post rainy (August–
markets, providing opportunities for cultural, social and October) seasons of 3 consecutive years (2009–2011).
technical education in improving the quality of family and
community life by increasing income. Morphological Studies of Fruit Bodies
Despite importance of these mushrooms among the tri-
bal people in the study area, they have been rarely scien- Morphological parameters (characteristic features of
tifically characterised and documented. Nucleotide pileus, lamellae, stipe, spore morphology and colour) of
sequence determination using ClustalW software was per- each collected mushroom were assessed from fresh sam-
formed for molecular assessment of eight mushroom ples. Photographs were taken from natural habitat as well
samples (CN 1–CN 8) of the study area (persistent fungal as under laboratory conditions. Morphological character-
contamination and DNA degradation were hindrance for istics were described according to the methodology sug-
the rest) using Internal Transcribed Spacer (ITS; genomic gested by Largent and Stuntz [9]. Spore surface of different
fragment of rDNA) 1 and 2 primers [8]. Present investi- mushrooms were studied using Scanning Electron Micro-
gation is undertaken to provide a comprehensive knowl- scope (EVOÒ-40, CarlZeiss SMT Ltd.).
edge to the local tribal people regarding the value of 16
naturally growing wild edible mushrooms. The study use Assessment of Nutritive Value
morphological attributes of fruit bodies and spores to
delineate the taxa to genus level. It also covers their Total protein (extraction following Osborne [10] and esti-
nutritional aspects as well as economic benefits associated mation as per Lowry et al. [11]), carbohydrate [12] and fat
to local people. Furthermore, cultivation, cost effective [13] contents were quantified in each mushroom sample (in
preservation protocol and toxic effect for two mushroom each case 3 replicas were made and average data has been
genera are also discussed. presented).
123
Table 1 Morphological comparison of gill mushrooms among the collected samples
Sample Pileus Lamellae Stipe Spore
no.
Size in Colour Colour of gills Colour Size Veils Morphology Colour
diameter and attachment (length 9 diameter)
(cm) of stipe (cm)
CN 1 6.0–10.0 Centre-orange, Creamy white, Maize 8.0–12.0 9 0.8–0.12 Annulus and Sub-prolate, size: 28.51 ± 1.4 9 24.64 ± 1.2 lm, White
margin-yellow free yellow volva surface wrinkled, non utriculate
Value of Wild Mushrooms
present
CN 2 7.0–11.0 Off white White, free Pale white 9.0–18.0 9 1.0–2.0 Annulus and Sub-prolate, size: 51.2 ± 0.5 9 41.3 ± 0.4 lm, White
volva surface wrinkled, non utriculate
present
CN 3 – – – – – – Prolate-spheroidal, size: 6.1 ± 0.2 9 5.9 ± 0.2 lm Cocoa brown
CN 4 5.5–8.0 Creamy white, centre White White 3.0–5.0 9 1.0–2.0 Absent Prolate-spheroidal,, size: 9.7 ± 0.7 9 8.9 ± 0.6 lm, Light pink
blackish or greyish surface smooth, non-porate, closely covered by utricle
CN 5 4.0–10.0 Creamy white, centre White White 12.0–35.0 9 1.0–2.5 Annulus Oblate-spheroidal, size: 6.2 ± 0.7 9 6.7 ± 0.5 lm, Light pink
blackish or greyish present utriculate, porate, pore elongated
CN 6 1.2–2.0 Creamy white, centre White White 3.0–5.0 9 0.15–0.3 Absent Prolate-spheroidal, size: 18.3 ± 1.5 9 17.0 ± 1.2 lm, Light pink
blackish or greyish smooth, non-porate, loosely covered by utricle
CN 7 0.4–1.0 Creamy white, centre White White 0.5–1.5 9 0.1 Absent Prolate, size: 7.5 ± 0.5 9 5.5 ± 0.5 lm Light pink
blackish or greyish
CN 8 8.0–15.0 White with brown at Pinkish brown White 7.0–14.0 9 1.0–1.8 Volva present Prolate, size: 57.5 ± 0.5 9 5.5 ± 0.5 lm, smooth, Brownish-pink
centre non-utriculate
CN 9 3.0–5.0 White to pale grey Blackish Pale white 3.0–5.0 9 0.8–1.0 Annulus Prolate, size: 8.8 ± 0.7 9 6.0 ± 0.6 lm, surface Blackish brown
brown, free present smooth, non-utriculate
CN 10 – – – – – – Sub-prolate, size: 5.0 ± 0.2 9 4.2 ± 0.3 lm, Dull yellow brown
verrucose ornamentation, verrucae small to tobacco brown
CN 11 4.0–7.0 Off white to grey White, free Off white 4.0–8.5 9 0.3–0.4 Volva present Prolate-spherical, size: 8.9 ± 0.5 9 8.1 ± 0.5 lm, White
Author's personal copy
123
Author's personal copy
222 S. K. Das et al.
their willingness to participate in the survey. Each family under investigation) and animal products (fishes, egg,
comprises of 4–9 members (data collected from each mem- chicken, snails, wild animals and birds). The chief objective
ber). Age group of family members ranged from 7 to of the survey was to constitute an idea regarding the signif-
[70 years. All surveyed families were below poverty line as icance of mushroom consumption in relation to other foods
per Ministry of Health and Family Welfare, Government of by taking local inhabitants in account. Data were collected
India (10th Five Year Planning—Rural) and mostly (98.0 %) during mushroom growing season (June–October) as well as
did not possess any primary school education. during pre- (April–May) and post (November–March)
Foods consumed were broadly classified into categories mushroom growing seasons in the year 2009, 2010 and 2011.
namely, pulses (seeds of lentil, gram, pea, pigeon pea etc.),
tubers (potato, yam, arum etc.), leafy vegetables (different Market Survey
leaves specifically arum), fruit vegetables (brinjal, tomato,
pumpkin, fig, gourd, etc.), legumes (beans, yard long beans Eight markets namely, Jhargram, Belpahari and Gidhni of
etc.), mushrooms (fruit bodies of the collected mushrooms Paschim Medinipur, Khatra, Ranibandh and Bankura of
123
Author's personal copy
Value of Wild Mushrooms 223
Bankura and Baghmundi and Barabazar of Purulia districts (tissues were preserved in 10 % formalin) to assess any
were surveyed each day during mushroom availability abnormalities associated with them.
period for 3 consecutive years. During market survey it was
specially noted about the type, quantity and price variation Mushroom Cultivation
of mushroom sold. Data was directly obtained from
mushroom selling vendors of the markets. Vendors as well Mycelia were developed from fruit bodies (pieces of
as consumers both represent ethnic group of people who 5 mm 9 2 mm samples were taken from internal portion
are below poverty line. and grown under aseptic condition) for all samples in malt
agar media (2 % malt extract and 2 % agar powder). The
Toxicity Analysis mycelia of each mushroom were inoculated in sterile wheat
media (grains mixed with 2 % calcium sulphate and 4 %
Experiment calcium carbonate in 1:3 ratio, pH 7.8) for spawn devel-
opment in polythene bags as well as in glass containers.
Sub-chronic toxicity test (28th day from dose initiation) was Paddy straw was used as the substrate material for fruit
conducted for two mushrooms (CN 5—Termitomyces sp. 2 body development. For this purpose 2.5 kg fresh dry paddy
and CN 12—Lentinus sp.) with Wistar rats in accordance to straw was cut into pieces of 18 inch length. The straw was
the schedule Y of drugs and cosmetic act (1940) to assess, then soaked in sufficient fresh water for 12 h. Excess water
the adverse effects caused due to mushroom consumption. from the straw was removed by spreading on sieve made of
The two most frequently found and widely consumed fine wire mesh. One bed with four layers was prepared by
mushrooms Termitomyces sp. and Lentinus sp. were the soaked straw. The straw pieces were placed in opposite
assessed. Both mushrooms are sold in market at relatively direction in each layer. The bed was placed on a clean
low to moderate price rate (Rs. 40–100/kg). The experiment polythene paper in a shaded semi-dark condition (temper-
involved 24 male and female rats (6–8 weeks old). Doses of ature 27 ± 1 °C, relative humidity 70–80 %). The small
0, 1000, 2000 and 4,000 mg/kg in the dose volume of 1 ml/ pieces (10–15 g) of spawn were placed (inoculation) in
100 g body weight (6 animals/sex/dose) were administered each layer through the side line of the bed. Total 200 g
orally. The animals were allowed 7 days period to accli- spawn was inoculated in a bed. The bed was covered by
matise with laboratory conditions prior to dosing. Rats were transparent polythene. The growth of the mycelium was
grouped into six per cage, sex wise and the individual observed. After 15th day of inoculation the polythene was
animal was fur marked with picric acid. The females were removed. Mature fruit body was developed after
nulliparous and not pregnant. The cages were polycarbon- 25–27 days of inoculation.
ated and provided with husk bedding (temperature
20–24 °C; humidity 30.0–70.0 %; 12 h dark and 12 h light Mushroom Preservation
cycles maintained).
For preservation of wild mushrooms 3 cost effective
Investigation methods were followed (1) mushrooms were preserved
directly in powdered salt and in different concentrations (5,
All animals were observed twice daily for mortality during 10, 15 and 20 %) of NaCl, (2) preserved in 100 % pure
the period of study. Blood samples were collected in the mustard oil in glass bottle and exposed under sunlight
morning (fasted over night) from orbital sinus using hep- during day time and (3) collected mushrooms were dried
arin as anticoagulant. under direct sunlight. Quality of the preserved mushroom
samples was assessed by organoleptic method following
Necropsy cooking at an interval of 10–12 days.
123
Author's personal copy
224 S. K. Das et al.
Fig. 2 Fruit bodies of mushrooms. a CN 1: Amanita sp. 1, b CN 2: j CN 10: Calvatia sp., k CN 11: Amanita sp. 3, l CN 12: Lentinus sp.,
Amanita sp. 2, c CN 3: Astraeus sp., d CN 4: Termitomyces sp. 1, m CN 13: Russula sp. 1, n CN 14: Russula sp. 2, o CN 15: Russula sp.
e CN 5: Termitomyces sp. 2, f CN 6: Termitomyces sp. 3, g CN 7: 3, and p CN 16: Russula sp. 4
Termitomyces sp. 4, h CN 8: Volvariella sp., i CN 9: Agaricus sp.,
showed globose, sub-epigenous, sessile fruit body, mushroom samples possessed outer peridium, endoperidium
2.5–3.0 cm in diameter, splitting to become star shaped, and gleba. On the basis of morphological characterisation, it is
covered with thin, white mycelial layer when unexpanded; it concluded that CN 3 and CN 10 are Astraeus sp. (Family:
tears away at maturity, often partly encrusted with soil debris; Diplocystaceae) and Calvatia sp. (Agaricaceae) respectively.
with slight odour when fresh. Fruit body of CN 10 was sub- Morphological characterisation of mushroom samples
globose, cushion shaped to turbinate, 4.0–6.0 cm tall, indicate that some samples belong to same genus (CN 1,
6.0–8.0 cm broad, abruptly tapered towards the base, some- CN 2 and CN 11—Amanita spp.—family: Amanitaceae;
times with a root like attachment to the substrate. Both the CN 13–16—Russula spp.—Russulaceae; CN 4–7—
123
Author's personal copy
Value of Wild Mushrooms 225
Fig. 3 Scanning electron micrographs of different mushroom spores a CN 6: Termitomyces sp. 3, b CN 9: Agaricus sp., c CN 11: Amanita sp. 3,
d CN 13: Russula sp. 1, e CN 15: Russula sp. 3, and f CN 16: Russula sp. 4
Termitomyces spp.—Lyophyllaceae). They could be different species (pileus orange at centre, yellow at margin) resembles
at species level. From the ecological point of view Amanita sp. A. hemibapha morphologically excepting pileus colour. Jana
1 and 2, Astraeus sp., Calvatia sp., Amanita sp. 3, and Russula and Purkayastha [15] collected R. delica from a market of
sp. 1–4 show ectomycorrhizal association with Shorea Medinipur. Edible fleshy fungi were also reported and
robusta; while, Termitomyces sp. 1–4 are symbiotic with described earlier [16–20]. CN 6 and CN 8 are found to match
termites. Sample CN 8 (Volvariella sp.—Pluteaceae) and CN with the morphological description provided by Johnsy et al.
12 (Lentinus sp.—Polyporaceae) are lignicolous and CN 9 [21] for T. microcarpus and Volvariella volvacea respectively.
(Agaricus sp.—Agaricaceae) is saprotrophic in nature. Roy Although modern identification is quickly becoming molec-
and Samajpati [14] reported 4 Russula spp. and A. hemibapha ular, the standard method for identification up to genus level
(white form) from Bankura district, West Bengal, adjoining to may still be accomplished by using morphological guidelines,
the location of present study. CN 1 identified as Amanita and can easily be disseminated among people.
123
Author's personal copy
226 S. K. Das et al.
Table 3 Elements present in fruit body (mg/kg dry weight) of different mushrooms
Sample no. Mushroom Cu Fe Zn Na K Mg Ca Pb Cd As
CN1 Amanita sp. 1 13.2 268.6 15.27 63.63 668.1 532 120 0.38 0.47 0.14
CN2 Amanita sp. 2 14.5 228.7 14.51 153.72 394.9 296 173 0.52 0.77 0.55
CN3 Astraeus sp. 4.2 159.1 13.38 244.65 427.3 280 276 0.28 0.04 0.65
CN4 Termitomyces sp. 1 15.2 327.5 12.70 539.50 458.8 630 252 0.17 0.26 0.24
CN5 Termitomyces sp. 2 16.7 476.0 12.36 573.90 180.9 610 350 0.57 0.43 0.27
CN6 Termitomyces sp. 3 21.3 241.0 10.18 475.13 150.8 495 214 0.42 0.50 0.52
CN7 Termitomyces sp. 4 20.4 350.0 9.04 483.23 110.9 456 245 0.46 0.47 0.45
CN8 Volvariella sp. 14.4 170.8 10.06 571.40 190.7 602 420 0.77 0.12 0.62
CN9 Agaricus sp. 14.7 144.0 9.56 277.56 170.6 502 390 0.47 0.11 0.52
CN10 Calvatia sp. 4.5 146.7 14.50 203.45 418.6 265 234 0.23 0.03 0.56
CN11 Amanita sp. 3 16.3 345.9 18.16 378.20 486.4 520 150 0.19 0.32 0.60
CN12 Lentinus sp. 7.9 397.3 10.13 512.78 190.9 501 125 0.33 0.37 0.50
CN13 Russula sp. 1 20.3 386.6 15.45 430.20 482.5 280 190 0.16 0.32 0.93
CN14 Russula sp. 2 13.5 135.4 7.73 347.08 444.3 320 180 0.15 0.35 0.91
CN15 Russula sp. 3 18.6 180.8 13.04 443.10 488.6 480 210 0.11 0.26 0.80
CN16 Russula sp. 4 16.5 201.3 12.06 360.73 355.7 380 220 0.37 0.12 0.70
123
Author's personal copy
Value of Wild Mushrooms 227
Economic Benefits
Market Survey
123
Author's personal copy
228 S. K. Das et al.
Culture raised fruit bodies of Volvariella sp. resemble 50 ppm ? KH2PO4 50 ppm). In the present investigation,
phenotypically to the described [30] paddy straw mush- it is rather surprising that fruit bodies of Agaricus sp. and
room V. volvacea. Thiribhuvanamala et al. [31] reported Lentinus sp. did not develop. Colak et al. [32] reported
maximum yield (927.8 g/bed; biological efficiency of wheat straw and waste tea leaves based compost for cul-
23.8 %) of V. volvacea in circular compact bed method. tivation of Agaricus bisporus. Reddy et al. [33] suggested
Oil palm waste in combination with rice straw or alone water hyacinth to be a good substrate for white button
recorded significantly higher yield of the mushroom. Yield mushroom (A. bisporus) production. Atri and Lata [34]
of fruit bodies was also enhanced by spraying bed with reported that Lentinus cladopus could be successfully
micronutrient booster VVB1 (CaCO3 400 ppm ? CaCl2 cultivated using wheat straw, paddy straw and mixture of
123
Author's personal copy
Value of Wild Mushrooms 229
Hematological investigation
Hb (g%) 13.9 13.9 13.4 13.4 13.5 13.7 13.9 14.1 14.3 14.6 13.6 13.5 14.7 14.5 13.1 14.0
Total RBC (9106/ 6.4 6.3 6.0 6.2 6.5 6.4 6.1 6.6 5.7 5.6 5.6 5.1 5.8 5.7 6.0 6.2
cmm)
Rt (%) 1.5 1.4 1.3 1.3 1.5 1.3 1.3 1.4 1.1 1.2 1.2 1.3 1.0 1.1 1.2 1.3
HCT (%) 40.2 40.6 39.9 39.3 39.5 38.8 40.5 41.8 42.8 44.3 41.5 41.2 44.2 43.8 39.8 42.4
MCV (lm3) 64.0 65.3 67.2 64.3 62.0 61.7 67.2 63.7 76.3 80.2 75.8 81.1 77.7 77.6 67.7 70.4
MCH (pg) 22.1 22.3 22.6 21.9 21.1 22.0 23.1 21.4 25.5 26.4 24.9 26.6 25.7 25.6 22.4 23.2
MCHC (%) 40.2 34.2 33.7 34.1 34.2 35.5 34.3 33.7 42.8 33.0 32.8 32.8 33.1 33.0 33.0 32.9
5
Platelets (910 / 5.8 6.4 6.2 6.8 6.1 6.6 6.5 6.8 7.2 7.1 7.3 7.7 7.4 7.1 7.7 7.7
cmm)
Total WBC 6.4 6.6 6.8 6.6 6.7 6.4 6.7 6.6 6.9 6.7 6.2 6.5 6.7 6.2 6.5 6.3
(9103/cmm)
Neutrophils (%) 23.5 25.0 24.3 27.2 24.3 22.8 24.2 24.8 20.8 21.7 21.3 21.7 21.5 22.5 22.5 22.2
Eosinophils (%) 2.0 2.2 1.5 1.5 2.5 1.5 1.7 1.8 3.0 3.2 3.0 3.2 2.8 3.0 3.2 3.2
Lymphocyte (%) 73.2 71.7 73.0 70.2 71.5 74.8 73.3 72.5 76.7 75.3 75.5 75.0 75.8 74.7 75.0 74.2
Monocyte (%) 1.3 1.2 1.2 1.0 1.7 0.8 0.8 0.8 0.3 0.7 0.8 0.7 0.7 0.7 0.7 0.7
Biomedical investigation
Total serum 6.2 6.2 6.1 6.1 6.3 6.1 6.5 6.2 6.3 6.6 6.4 6.4 6.4 6.6 6.4 6.4
protein (g%)
BUN (mg%) 27.2 30.2 28.7 26.7 26.8 25.5 29.3 26.3 20.2 22.7 24.3 26.7 20.8 21.5 24.3 26.5
SGPT (IU/l) 50.7 58.8 58.5 58.2 44.2 68.8 63.2 34.7 53.3 55.0 67.0 66.7 56.3 60.0 67.0 63.0
SGOT (IU/l) 85.3 100.5 92.3 96.2 113.5 104.0 99.0 97.5 93.3 95.3 98.0 98.2 91.8 97.0 98.1 103.0
SAP (IU/l) 331.7 312.3 289.3 319.3 291.7 308.3 343.7 309.5 269.5 294.2 310.7 324.7 291.2 301.5 310.7 327.8
Blood sugar 101.8 99.8 94.0 91.8 95.8 83.8 97.0 94.0 90.7 91.0 94.8 92.8 93.3 93.8 93.3 95.2
(mg%)
Organ weight
Bodyweight (g) 109.5 110.7 111.2 110.9 112.2 111.3 109.3 112.4 106.5 106.9 104.0 105.5 107.0 105.1 106.1 105.2
Liver (g) 5.0 4.7 4.3 4.1 4.2 4.4 4.4 4.2 4.4 4.5 4.4 4.9 4.6 4.6 4.7 4.7
Kidney (g) 0.8 0.8 0.8 0.7 0.8 0.8 0.8 0.8 0.8 0.8 0.7 0.8 0.8 0.7 0.8 0.8
Heart (g) 0.4 0.4 0.4 0.4 0.4 0.4 0.4 0.4 0.4 0.5 0.5 0.5 0.5 0.5 0.5 0.5
Hb Hemoglobin, RBC red blood corpuscles, Rt reticulocyte, HCT hematocrit, MCV mean corpuscular volume, MCH mean corpuscular
hemoglobin, MCHC mean corpuscular hemoglobin concentration, WBC white blood corpuscles, BUN blood urea nitrogen, SGPT serum glutamic
pyruvic transaminase, SGOT serum glutamic oxaloacetic transaminase, SAP serum alkaline phosphatase
123
Author's personal copy
230 S. K. Das et al.
Fig. 6 Histological
preparations of a heart, b liver,
c lung, and d kidney in Wistar
Rat showing normal cellular
architecture at high dosing
(4,000 mg/kg) under light
microscope (CV central vein,
HC hepatic cell, KC kupffer
cell, A alveoli, DT distal
tubules, G glomerulus)
up to 6 months without any bad odour and taste. Mushrooms different dose (1,000–4,000 mg/kg) groups (male:
(Amanita sp. 2, Astraeus sp., Termitomyces sp. 1–4) can also 97.60–99.13 to 110.67–110.85 g, female: 98.22–98.52 to
be preserved in powdered salt (Fig. 5c) and in salt concen- 109.33–112.43 g; male: 102.97–102.85 to 106.92–105.47 g,
trations (Fig. 5d) up to 3 months without affecting their female: 100.65–102.53 to 105.08–105.15 g) exhibit normal
taste; however, depending on salt concentrations they are body weight gain throughout the dosing period (data recor-
slightly salty. Sun drying (Fig. 5f) of mushrooms (Termito- ded at an interval of 7 days).
myces sp. 2 and Lentinus sp.) is also found to be effective for Data scored for hematological and biochemical param-
preservation up to 6 months without any deterioration. eters of both male and female rats following consumption
Therefore, simple and cost effective measures of mushroom of mushrooms on termination of dosing have been pre-
preservation may boost consumption of mushroom as an sented in Table 4. As compared to control, the data scored
important food resource for common people. Martinez- from dosing animals did not show any significant changes
Carrera et al. [40, 41] reported acidified Mexican recipes and and are within normal biological and laboratory limits.
canning in glass containers as preservation techniques of The gross pathological examination of necropsy
wild edible mushroom from central Mexico. revealed no abnormality associated to treatment. Further,
histopathological examination of heart, liver, kidney and
Toxicity Analysis lungs of both sexes of animals at high dose group showed
normal architectural and cellular arrangement without any
All animals from control and different dose groups survived cellular damages or necrosis (Fig. 6a–d). Although only
throughout the dosing period (1–28 days) showing no mor- two mushrooms are studied, results indicate the safe con-
tality. Animals from control (Termitomyces sp.—male: sumption of mushroom as food supplement/major food.
93.32–109.52 g, female: 93.13–112.20 g; Lentinus sp.— Thus, proper knowledge regarding wild edible mush-
male: 101.42–106.53 g, female: 102.43–107.03 g) and the rooms among the poor, uneducated local tribal people may
123
Author's personal copy
Value of Wild Mushrooms 231
be significant for understanding the value of the mush- 17. Upadhyay RC, Kaur A (2004) New records and taxonomy of
rooms in their socio-economic life. However, natural and Agaricales (Tricholomataceae) from North-Western Himalaya.
J Mycol Plant Pathol 34:194–199
anthropological declination in forest area seems to be 18. Tang BH, Wei TZ, Yao YJ (2005) Type revision of three Ter-
hindrance in this regard. In this context, Government pol- mitomyces species from India. Mycotaxon 94:93–102
icy must be framed regarding domestication and commer- 19. Srivastava B, Dwivedi AK, Pandley VN (2011) Morphological
cialisation of wild edible mushrooms in collaboration with characterisation and yield potential of Termitomyces spp. mush-
room in Gorakhpur forest division. Bull Env Pharmacol Life Sci
non-governmental organisations to harvest benefits of the 1:54–56
tribal people. 20. Vishwakarma MP, Bhatt RP, Gairola S (2011) Some medicinal
mushrooms of Garhwal Himalaya, Uttarakhand, India. Int J Med
Acknowledgments The research is grant aided by Science and Arom Plants 1:33–40
Society Division, Department of Science and Technology, Govern- 21. Johnsy G, Davidson SD, Dinesh MG, Kaviyarasan V (2011)
ment of India. Nutritive value of edible wild mushrooms collected from the
Western Ghats of Kanyakumari District. Bot Res Int 4:69–74
Conflict of interest The authors declare that they have no conflict 22. Alofe FV, Odeyemi O, Oke OL (1996) Three edible wild
of interest. mushrooms from Nigeria: their proximate and mineral compo-
sition. Plant Foods Hum Nutr 49:63–73
23. Adejumo TO, Awosanya OB (2005) Proximate and mineral
composition of four edible mushroom species from South Wes-
tern Nigeria. Afr J Biotechnol 4:1084–1088
References 24. Lee CY, Park JE, Kim BB, Kim SM, Ro HS (2009) Determi-
nation of mineral components in the cultivation substrates of
1. Jonathan SG, Fasidi IO (2003) Antimicrobial activities of two edible mushrooms and their uptake into fruiting bodies. Myco-
Nigerian edible macrofungi—Lycoperdon pusillum (Bat. Ex) and biology 37:109–113
Lycoperdon giganteum (Pers). Afr J Biomed Res 6:85–90 25. Ayodele SM, Odogbili OD (2010) Metal impurities in three
2. Sanmee R, Dell B, Lumyong P, Izumori K, Lumyong S (2003) edible mushrooms collected in Abraka, Delta State, Nigeria.
Nutritive value of popular wild edible mushrooms from northern Micol Aplicada Int 22:27–30
Thailand. Food Chem 82:527–532 26. Manjunathan J, Kaviyarasan V (2011) Nutrient composition in
3. Gbolagade J, Fasidi I (2005) Antimicrobial activities of some wild and cultivated edible mushroom, Lentinus tuber-regium (Fr.)
selected Nigerian mushrooms. Afr J Biomed Sci 8:83–87 Tamil Nadu, India. Int Food Res J 18:784–786
4. Manna S, Roy A (2014) Economic contribution of wild edible 27. Uzun Y, Genccelep H, Kaya A, Akcay ME (2011) The mineral
mushrooms to a forest fringe ethnic community in some eastern contents of some wild edible mushrooms. Ekoloji 20:6–12
lateritic parts of India. J For Res 19:52–61 28. Härkönen M (1998) Uses of mushrooms by Finns and Karelians.
5. Manoharachary C, Sridhar K, Singh R, Adholeya A, Suryana- Int J Circumpolar Health 57:40–55
rayanan TS, Rawat S, Johri BN (2005) Fungal biodiversity: dis- 29. Ortega-Martı́nez P, Martı́nez-Peña F (2008) A sampling method
tribution, conservation and prospecting of fungi from India. Curr for estimating sporocarps production of wild edible mushrooms
Sci 89:58–71 of social and economic interest. Invest Agrar Sist Recur For
6. Tibuhwa DD (2013) Wild mushroom—an underutilized resource 17:228–237
for healthy food and income generation: experience from Tan- 30. Kilchling P, Hansmann R, Seeland K (2009) Demand for non-
zania rural areas. J Ethnobiol Ethnomed 9:49 timber forest products: surveys of urban consumers and sellers in
7. Arora D (1986) Mushrooms demystified. Ten Speed Press, Switzerland. For Policy Econ 11:294–300
Berkeley, p 23 31. Thiribhuvanamala G, Krishnamoorthy S, Manoranjitham K,
8. Das SK, Mandal A, Datta AK, Gupta S, Paul R, Saha A, Sengupta Praksasm V, Krishnan S (2012) Improved techniques to enhance
S, Dubey PK (2013) Nucleotide sequencing and identification of the yield of paddy straw mushroom (Volvariella volvacea) for
some wild mushrooms. Sci World J 2013. Article ID 403191. doi: commercial cultivation. Afr J Biotechnol 11:12740–12748
10.1155/2013/403191 32. Colak M, Baysal E, Simsek H, Toker H, Yilmaz F (2007) Cul-
9. Largent DL, DE Stuntz (1986) How to identify mushrooms to tivation of Agaricus bisporus on wheat straw and waste tea leaves
genus I: macroscopic features. Revised edn. Mad River Press, based composts and locally available casing materials Part III:
Eureka, pp 1–166 dry matter, protein, and carbohydrate contents of Agaricus
10. Osborne DJ (1962) Effect of kinetics on protein and nucleic acid bisporus. Afr J Biotechnol 6:2855–2859
metabolism in Xanthium leaves during senescence. Plant Physiol 33. Reddy NM, Reddy AKK, Reddy AK, Reddi UBE, Reddi BT
37:595–602 (2013) A study on the production of Agaricus bisporus mush-
11. Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ (1951) Protein rooms using Eichhornia crassipes (Mart. Solms)—a troublesome
measurement with the Folin phenol reagent. J Biol Chem exotic aquatic weed of Kolleru lake. Int J Sci Nat 4:100–103
193:265–275 34. Atri NS, Lata (2013) Studies for culturing and cultivation of
12. Hedge JE, Hofreiter BT (1962) Carbohydrate chemistry, 17th Lentinus cladopus Lév. Mycosphere 4:675–682
edn. Academic Press, New York 35. Upadhyay RC, Sohi HS (1988) Apple pomace—a good substrate
13. Bligh EG, Dyer WJ (1959) A rapid method of total lipid for the cultivation of edible mushrooms. Curr Sci 57:1189–1190
extraction and purification. Can J Biochem Physiol 37:911–917 36. Upadhyay RC, Sohi HS (1989) Natural occurrence of Stropharia
14. Roy A, Samajpati N (1980) Agaricales of West Bengal—III. rugosoannulata Farlow Apud Murrill in Himachal Pradesh
Indian J Mycol Res 18:13–24 (India) and its artificial cultivation. Mus Sci 12:509–516
15. Jana KK, Purkayastha RP (1982) A new record of edible Russula 37. Banerjee P (1994) Successful isolation and growth of tissue
from India. Curr Sci 51:844 cultures of Pluteus species. Mycologist 8:32–35
16. Upadhyay RC, Kaur A (2003) New addition to the Indian fleshy 38. Ghazala N, Malik SH, Rukhsana B, Afzal M, Mian SW (2001)
fungi from North Western Himalaya. Mus Res 12:9–14 Effect of three different culture media on mycelial growth of
123
Author's personal copy
232 S. K. Das et al.
oyster and Chinese mushrooms. Online J Biol Sci cultivated edible mushrooms in rural conditions in Mexico.
1(12):1130–1133 Micologı́a Neotropical Aplicada (Mexico) 9:15–27
39. Pani BK (2011) Effect of spawning methods on sporophore 41. Martinez-Carrera D, Sobal M, Aguilar A, Navarro M, Bonilla M,
production of Calocybe indica. Biosci Discov 2:189–190 Larque SA (1998) Canning technology as an alternative for
40. Martinez-Carrera D, Vergara F, Juárez S, Aguilar A, Sobal M, management and conservation of wild edible mushrooms in
Martı́nez W, Larqué SA (1996) Simple technology for canning Mexico. Micologı́a Neotropical Aplicada (Mexico) 11:35–51
123