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USE OF GLUCOSE
• Energy – glucose is the primary fuel for most
cells in the body. The brain MUST have
glucose!
• Sparing body protein – if glucose is scarce,
the body will breakdown its own protein.
• Preventing ketosis – with no carbohydrate, fat
breakdown produces ketone bodies. Can
lead to ketosis.
• Storage as glycogen – liver stores are used
to maintain blood sugar, while muscle stores
are used to fuel activity.
Introduction to Carbohydrates
Alcohols
• Alcohol is an organic compound containing the
–OH group.
• Ethanol is one of the simplest alcohols and is
prepared from the fermentation of simple sugars
in grains and fruits. Ethanol is present in beer
and liquors, and is used as an alternative fuel
blend, such as gasohol and E85 (85% ethanol
and 15% gasoline).
Aldehydes
• Monosaccharides can
contain an aldehyde group
on one end of the molecule
in addition to multiple
hydroxyl groups.
Ketones
• A ketone also contains the carbonyl group, but
has an alkyl or aromatic group on both sides of
the carbonyl group.
• Acetone is the simplest ketone. It is the main
component of fingernail polish remover.
Stereochemistry in Monosaccharides
Representing stereoisomers—the
Fischer projection
• Enantiomers are written as if there is a
mirror placed between the two molecules.
• Enantiomers of D- and L-glucose are:
H H H H
C O C O C O C O
H C OH HO C H H C OH HO C H
HO C H H C OH H C OH HO C H
H C OH HO C H H C OH HO C H
H C OH HO C H H C OH H C OH
a and b forms
spontaneously
interconvert in an
aqueous environment.
• Proportion of each
anomer differs for each
sugar.
Ring Formation—The Truth about
Monosaccharide Structure
• Carbonyl groups can also react with a hydroxyl
functional group (–OH).
H C O H C OH
H C OH H C OH
O
HO C H HO C H
H C OH H C OH
H C OH H C
CH2OH CH2OH
Ring Formation—The Truth about
Monosaccharide Structure, Continued
A hemiacetal can form within a monosaccharide
since it contains both a carbonyl and several
hydroxyl functional groups.
Glycosidic
linkage
Aglycone
Disaccharides, Continued
Glycosidic
linkage
Aglycone
Disaccharides, Continued
Maltose
• Maltose is known as malt sugar.
• It is formed by the breakdown of starch.
• Malted barley, a key ingredient in beer,
contains high levels of maltose.
• During germination of barley seeds, the starch
goes through hydrolysis to form maltose. This
process is halted by drying and roasting
barley seeds prior to their germination.
• One of the anomeric carbons is free, so
maltose is a reducing sugar.
© 2011 Pearson Education, Inc. Chapter 5 57
Disaccharides, Continued
Maltose, Continued
• The glycosidic bond is α(1→4).
Lactose
• Lactose is known as milk sugar.
• It is found in milk and milk products.
• An intolerance to lactose can occur in people
who inherit or lose the ability to produce the
enzyme lactase that hydrolyzes lactose into
its monosaccharide units.
• The glycosidic bond is b(1→4).
• One of the anomeric carbons is free, so
lactose is a reducing sugar.
Sucrose
• Sucrose is known as table sugar.
• It is the most abundant disaccharide found in
nature.
• Sucrose is found in sugar cane and sugar
beets.
• The glycosidic bond is b (1→2).
• Both anomeric carbons of the
monosaccharides in sucrose are bonded,
therefore, sucrose is not a reducing sugar. It
will not react with Benedict’s reagent.
© 2011 Pearson Education, Inc. Chapter 5 61
Disaccharides, Continued
• 3 - 10 or more subunits
• N-linked
– Attached via asparagine.
• O-linked
– Attached via threonine or
serine or OH on lipid
Polysaccharides
Polysaccharides
Glycogen
• Glycogen is a storage polysaccharide found in
animals.
• Glycogen is stored in the liver and muscles.
• Its structure is identical to amylopectin, except
that α(1→6) branching occurs about every
12 glucose units.
• When glucose is needed, glycogen is
hydrolyzed in the liver to glucose.
Structural Polysaccharides
Cellulose
• Cellulose contains glucose units bonded
b(1→4).
• This glycosidic bond configuration changes the
three-dimensional shape of cellulose compared
with that of amylose.
• The chain of glucose units is straight. This
allows chains to align next to each other to form
a strong rigid structure.
© 2011 Pearson Education, Inc. Chapter 5 71
Polysaccharides, Continued
Cellulose
• Cellulose is an insoluble fiber in our diet
because we lack the enzyme cellulase to
hydrolyze the b(1→4) glycosidic bond.
• Whole grains are a good source of cellulose.
• Cellulose is important in our diet because it
assists with digestive movement in the small and
large intestine.
• Some animals and insects can digest cellulose
because they contain bacteria that produce
cellulase.
© 2011 Pearson Education, Inc. Chapter 5 73
Polysaccharides, Continued
Chitin
• Chitin makes up the exoskeleton of insects and
crustaceans and cell walls of some fungi.
• It is made up of N-acetylglucosamine containing
b(1→4) glycosidic bonds.
• It is structurally strong.
• Chitin is used as surgical thread that
biodegrades as a wound heals.
• It serves as a protection from water in insects.
• Chitin is also used to waterproof paper, and in
cosmetics and lotions to retain moisture.
© 2011 Pearson Education, Inc. Chapter 5 74
Polysaccharides, Continued
• Glycosaminoglycans
(GAGs)
• Heteropolysaccharides
– Disaccharide repeating
units
• Negatively charged at
physiological pH.
Heparin