Professional Documents
Culture Documents
2) 6.5% Nacl Broth= Only Enterococci grow, Group D Strep Does not.
3) Optochin Disk = Strep. Pneumoniae is sensitive, Strep Viridian is Resistant. Remember (oPtochin has letter P and pneumonia also
starts with P )
Listeria= Cold Enrichment, Tumbling Motility, Umbrella Growth. Motile at 25 degree Centigrade but not at 37 degre. The only Motile
B. Anthracis= Spore Forming, Ground Glass Bambmoo shoot like structure, Medussa Head, Causes Anthrax and cutaneous ulcer
B Cereus = So this Organism is responsible for Food Poisoning, Toxin Fried Rice. (Just Remember Morning Cereal as food and name
of this organism is also Cereus similar name and it causes Food poisoning)
Erysipelothrix= H2S+, So causes skin infection in Fisherman or Butchers or rose Growers, In short it causes Occupational infection.
Corynebacterium= Chinese letter like, Black Colonies on Tindale Agar or Loeffler Agar Medium. , Confirm by growth in Potassium
Tellurite Agar. Highly Toxic Organism. Need to confirm toxicity by ELEK test to check if organism is a producer of toxin, if it produces
Klebiseilla has 2 types and can be differentiated on the basis of Indole Test. Kleb Oxytoca is Indole + and Kleb Pneumina is Indole -, How will
you remeber which one is Ibdole (+)—-> just remember Oxytoca like oxygen and so indole +
Remember Proteus has 2 types and can be differentiated on the basis of Indole Test
So how do I remember which is positive, just compare Vulgaris with Volcano and its hot and firy and so Indole +
A on Top means ferment Lactose or Sucrose, A on Bottom means Ferment Glucose. A/A = Ferment through out Slant (Body) and Butt (Bottom)
K/A= means does not ferment Lactose but just Ferment Glucose.
Colum 1 =A/A = EEKY = first 3—> EEK are Lactose Fermenter and Yersenia just Ferment Sucrose, so the Agar plate with EEK will be pinkish but
3rd column = K/A= Non Lactose Fermenter, just Glucose Fermenter and does not produce H2S (negative)= SS-PP-M
4th Column= K/A and H2S+= Saci-Pred= Salmonella, Proteus and Mr. Edward
I just kept Citrobacter so you can remeber that Saci-Pred= H2S+ but Citrobacter had already been placed in column 2 bcos its slow lactose
Column 5= K/K= zero nothing, No fermentation and No H2S=Pseudomonas. Only Organism which is Oxidase + among this group.
Special case: But remember not all of providencia Species are Urease (+) only and only Providencia Rettgeri among the Providencia Species
7) Oxidase + = The ones which end with (-onas)= Pseudomonas and Pleisimonas.
8) H2S+= Saci-PrEd= sexy predator= Sal, Citro, Proteus and Mr. Edwardsiella.
9) Non Motile = The ones ending with (-ella)= Klebsiella and Shigella :)_
Remember how Klebsieilla is pronounced Kleb-siiieeellla takes a long time to read so this organism is very mucoid and cannot move bcos it
10) Indole + = E coli , K Oxytoca (remember as Oxygen), P. Vulgaris (Volcano), Mr. Edward and Mrs Morgan (they both work at my Lab ) and
finally 2 of which start with P i.e—> Providenscia and Pleisimonas.
Table # 5 HACEK Group
Table # 6 Haemophilus Gram(-) CoccoBacillus
Summary of Table #6:
These Bacteria will always Grow on Choclate AGar and 10 % CO2 because it supplies both X and V Factors. They do not Grow on Blood Agar
because of NADase in the agar ( which inactivates NAD) but will grow on Horse or Rabbit Agar which contains no NADase.
Exception on Growing in Blood Agar can happen only when this bacteria grow around colonies of S Aureus as S. Aureus produces NAD and
Hemolyse the Blood cells releasing Hemin as well and this Phenomenon is known as Satellitism.
Both H Influenzae and H. Haemolytics (Not Para’s) need both X and V Factor
Both Para (Para Influenz. and Para Haemolytia) need V (NAD) and don’t need (X)
H.Aphrophilus and H Ducreyi = Reactions are exactly opposite For H Aphro= – – – + and H Ducreyi = + – – –
Table # 7 Curved Gram (-) Bacilli AKA Vibrio
Table # 8 Gram (-) Diplococci
Summary:
Neisseria Species Grow on Choclate and selective Media ( MTM, Martin Lewis and NYC)
Lactamica = Starts with L and has M in the middle= SO Fermenrs Glucose + Maltose+ Lactose
Table # 9 Gram (+) Anaerobes
Table # 10 Gram (-) Anaerobes
Summary:
Fusobacterium = Fus Fus= Brain Abcess, spindle shaped (brain like structure so attack Brain)
Indole (+)= Fuso Bacterium and Porphyromas and Both organsims are sensitive to 2 Antibiotics
Table # 11 Zoonotic Diseases Acquired from Animals
Table # 12 Fastidious Gram (-) Rods (Pleomorphic)
Table # 13 Chlamydia and Rickettsiae
Culture Plate Agar and Their Uses For Memorization ( Very Important)
Culture Plate # 1
Culture Plate # 2
MicroBiology Review in a Nutshell :