LIST OF MOLECULAR BIOLOGY GRADE CHEMICALS TO BE PURCHASED

UNDER BIOTECH PROJECT

1. Abscisic Acid
Formula: C15H20O4
Molecular Wt: 264.32
Form: Powder
Appearance: White to Yellow Powder
Application: Plant Growth Regulator
Solubility: KOH

2. Acetone Ultrapure
DNase/RNase/Proteases not detectable
Acidty/alkalinity max. 0.0005meg/g
Assay (GC) minimum99.9%
Non-volatile matter max 0.0002%
3. Acetosyringone
Formula C10H12O4
Molecular Wt 196.20
Form Powder
Appearance Off-white to Tan
Application Molecular Biology
Solubility DMSO

4. Acrylamide - Solution (40 %) - Mix 19 : 1 Molecular biology grade B

Composition:
Acrylamide (A3812) . . . . . . . 380.0 g/L
Bisacrylamide (A3636) . . . . 20.0 g/L
5. L-Ascorbic Acid
Formula: C6H8O6
Molecular Wt: 176.13
Form: Powder
Appearance: White Crystalline
Application: Antioxident, Vitamin
Solubility: water

6. AGAR, plant TC micropropagation grade

Form Powder
Appearance Cream to Tan Powder
Gel Strength ≥ 700 g/cm2
Suitability Plant Tissue Culture Tested
Solubility boiling water
Ash Content <6%

7. Ampicillin
Assay 96.0-100.5% (anhydrous basis)
Melting point 208 °C (dec.)(lit.)
Solubility 1 M NH4OH: 50 mg/mL

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8. 6-Benzyl amino purine (BAP)
Linear Formula: C12H11N5
Molecular Weight: 225.25
Suitability plant cell culture grade

12. Boric acid

Formula BH3O3
M.W 61.83 g/mol
Melting point . . . . . . . . 185˚C (dec.)
Solubility (20˚C) . . . . . . 50 g/L (H2O)

13. Calcium Chloride Dihydrate

Formula: CaCl2•2H2O
Molecular Wt: 147.02
Form: Powder
Appearance: White powder
Application: Plant Macronutrient
Solubility: water

14. Casein, Enzymatic Hydrolysate

Form Powder
Appearance: Yellow to Beige Powder
Application Microbiology; Orchid Media Supplement
Solubility Water

15. Cefotaxime, Sodium Salt

Formula C16H16N5O7S2Na
Molecular Wt 477.4
Form Powder
Appearance White to Cream Powder
Application Plant Tissue Culture Antibiotic
Solubility Soluble in Water

21. DNA ladder 1kb

Ideal for both DNA sizing and approximate quantification
Sharp bands
Bright reference bands
Ready to use
6x DNA loading dye be supplied alongwith (pH=7.6)
No. of Fragment = 13 or above
Min. fragment = 250 bp

22. DNA transfer Stacks

DNA Transfer Stack containing sponges, cathode and anode electrodes and membrane, pre-assembled
for optimum transfer of DNA from gels to the membrane through Dry blotter
Gel Compatibility: Acrylamide Gels, Agarose Gels
Gel Size: 8 cm x 13 cm
Membrane Type: Nylon Membrane
Pore Size: 0 .2 µm

28. FeNa-EDTAEthylenediaminetetraacetic Acid,

Ferric-Sodium Salt
Formula: C10H12N2O8NaFe

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 Molecular Wt: 367.02
Form: Powder
Appearance: Green to Brown Crystalline
Application: Plant Micronutrient
Solubility: Water

30. Fluorescent Molecular Probe

TaqMan Probes:
Dye/Quencher
5' 6-FAM/3' Tamra (at 200 nmol scale with minimum yield of 20 nmol)
5' HEX/3' Tamra (at 200 nmol scale with minimum yield of 20 nmol)
5'-Cy 3 /3' Tamra (at 200 nmol scale with minimum yield of 10 nmol)
5'-Cy 5/3' Tamra (at 200 nmol scale with minimum yield of 10 nmol)

Dye/NFQ
5' 6-FAM (at 200 nmol scale with minimum yield of 20 nmol)
5' HEX (at 200 nmol scale with minimum yield of 20 nmol)
5'-Cy 3 (at 200 nmol scale with minimum yield of 10 nmol)
5'-Cy 5 (at 200 nmol scale with minimum yield of 10 nmol)

Price should include synthesis and purification of unmodified DNA oligo up to 32mer and reverse phase
purification with guaranteed yield for dye and quencher

31. Gamborg’s B-5 Basal Medium

Form powder
Suitability plant cell culture grade
Formula with the macro- and micronutrients, and vitamins as described by Gamborg, et al. (1968)
Solubility water

32. Gibberellic acid (GA3)

C19H22O6 M=346.37
Plant cell culture grade
Assay………… ≥90% gibberellin A3 basis (of total gibberellins.)

33. D-(+) Glucose Anhydrous

Formula C6H12O6
Molecular Wt 180.16
Form Powder
Appearance White Crystalline
Application Carbohydrate Source (plant cell culture)
Solubility Water

34. Glycerol
Formula C3H8O3
Molecular Wt 92.09
Form Liquid
Appearance Colorless, Clear Liquid
Solubility Miscible with Water

35. Hygromycin B
Formula C2OH37N3O13

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 Molecular Wt 527.5
Form Powder
Appearance White to yellow Powder
Application Plant Tissue Culture selection agent
Solubility Water, buffer, or other aqueous solution

36. Indole-3-Acetic Acid

Formula C10H9NO2
Molecular Wt 175.19
Form Powder
Appearance Cream to Pale Pink Powder
Application Auxin
Suitability Plant Tissue Culture Tested
Solubility KOH

37. Indole-3-Butyric Acid

Formula C12H13NO2
Molecular Wt 203.24
Form Powder
Appearance Cream to Tan Powder
Application Auxin
suitabilityPlant Tissue Culture Tested
Solubility KOH

40. IPTG (Isopropyl-b-Dthiogalactopyranoside)

Formula C9H18O5S
Molecular Wt 238.3
Form Powder
Appearance White Powder
Application Molecular Biology
Solubility Water

41. Kanamycin
Potency ≥750 μg per mg
Total impurities <5% kanamycin B
Suitability Plant cell culture grade

42. Kinetin
Formula C10H9N5O
Molecular Wt 215.2
Form: Powder
Appearance: White to Yellow Crystalline Powder
Application: Cytokinin
Solubility: KOH

43. D-Maltose Monohydrate

Formula C12H22O11•H2O
Molecular Wt 360.32
Form Powder
Appearance White Crystalline
Application Carbohydrate Source (plant cell culture)
Solubility Water

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45. Murashige and Skoog Salts with Vitamins
Form powder
Suitability plant cell culture grade
Formula with macro and micronutrients and vitamins as described by Murashige
& Skoog (1962)
Solubility water

46. myo-Inositol
Formula: C6H12O6
Molecular Wt: 180.16
Form: Powder
Appearance: White Powder
Application: Vitamin
Solubility: Water

47. Naphthalene acetic acid (NAA)

Assay ~97%
Form crystalline
Color light yellow
Suitability plant cell culture grade
Linear Formula: C12H10O2
Molecular Weight: 186.21

48. Nicotinic Acid

Formula: C6H5NO2
Molecular Wt: 123.12
Form: Powder
Appearance: White Fluffy Powder
Application: Vitamin
Solubility: Water

49. Plant RNA Reagents kit

Specifications
Suitable for high yield and quality of total RNA
Ideal for samples rich in polyphenolics or starch

51. Potassium Chloride

Formula: KCl
Molecular Wt: 74.55
Form: Powder
Appearance: White Crystalline
Application: Plant Macronutrient; Electrode Filling Solution; Molecular Biology
Solubility: Water

52. Phytagel
Form powder
Suitability plant cell culture grade

53. Pyridoxine Hydrochloride

Formula: C8H11NO3•HCl
Molecular Wt: 205.64
Form: Powder
Appearance: White Crystalline

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 Application: Vitamin
Solubility: water

54. Rifampicine
Assay ≥97% (HPLC)
Form powder
Color orange-brown to red-brown
Solubility H2O: 1.3 mg/mL pH 4.3
DMSO: 100 mg/mL
H2O: 2.5 mg/mL at 25 °C pH 7.3

55. RNA ladder 100bp

Ideal for RNA sizing and approximate quantification
Sharp bands
Bright reference bands
Ready to use
Supplemented 2x RNA loading dye
No. of Fragment = 7 or above
Minimum fragment size 100bp

56. RNA Plant extraction kit

Specification
Suitable for RNA isolation 1-10% from 10mg of plant tissue
Including filters for reduction of lysate viscosity
Suitable up to 70ug of pure RNA per prep
Including rDNase with buffer

57. 2X Long PCR Master Mix

The ready to use master mix should contain all the reagents (except primers, probe, and template) for
robust amplification of up to 6 kb from genomic DNA and up to 20 kb from viral DNA.

58. Silver nitrate Molecular biology grade

AgNO3 M = 169.87 g/mol
Boiling point………………444oC
Melting point……………212oC
Solubility (20oC)……….2150g/L (H2O)

DNases/RNases/Proteases free Fe…………..max. 0.0002 %

Assay (titr)…………………………..min. 99.8 % Mn…………max. 0.0005%
Chloride………………………………max. 0.0005 % Ni…………..max. 0.0005 %
Sulfate………………………………..max. 0.002 % Pb………….max. 0.001 %
Cd……………………………………….max. 0.0001 % Zn………….max. 0.0001 %

59. Sodium Chloride Molecular biology grade

NaCl M = 58.44 g/mol

Boiling point……………. 146oC

Melting point…………. 801oC
Solubility (20oC)…….. 358 g/L (H2O)

DNases/RNases/Proteases free
phosphate…. .max. 0.001%

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 k………. max. 0.005%
sulfate………. .max. 0.001%
Mg……. max. 0.001%
pH (5 %: H2O)… max. 5.0 – 8.0 (20oC)
Ba………………. max. 0.001%
Pb……. max. 0.0005%
Bromide……… max. 0.005%
Ca……. max. 0.002%
Iodide……………. max. 0.001%
Fe……. max. 0.0001%

60. Sodium dodicyl sulphate (SDS)

Grade for electrophoresis
Assay ≥98.5% (GC)
Mol wt 18,000
Aggregation number 62
Turbidity <2.5 NTU
CMC 7-10 mM(20-25°C)
Melting point 204-207 °C(lit.)
Transition temp cloud point >100 °C
Anion traces chloride (Cl-): ≤500 ppm
phosphate (PO4-): ≤10 ppm
Cation traces heavy metals (as Pb): ≤10 ppm
Absorbance A3%/260 ≤0.1
A3%/280 ≤0.1

61. Spectinomycin
Formula C14H24N2O72HCl5H2O
Molecular Wt 495.35
Form Powder
Appearance White to off-white Powder
Application Plant Tissue Culture Antibiotic
Solubility Water

62. Sodium hydroxide pellets Molecular biology grade

NaOH M = 40.00 g/mol
Boiling points……………… 1390oC
Melting point……………… 324oC
Solubility (20oC)………… 1090oC g/L (H2O)

DNases/RNases/Proteases free
phosphate…. .max. 0.0005%
K………. max. 0.05%
Assay (titr)…… min. 99 %
sulfate….max. 0.0005%
Mg…….max. 0.0005%
pH (1M: H2O)…………………… (20oC)
Ca……….max. 0.0005%
Pb…….max. 0.0005%
Carbonate……………… max. 1.0%
Cu………………. max. 0.0002%

63. D-Sorbitol
Formula C6H14O6

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 Molecular Wt: 182.17
Form: Powder
Appearance: White powder
Application: Carbohydrate Source; Molecular Biology
Solubility: water

64. Streptomycin
C28H26N4O3 M=466.53g/mol
Assay……….. ≥95%
Solubility……. DMSO: soluble
H2O: insoluble
Ethanol: soluble
Methanol: soluble

65. Sucrose
Grade for molecular biology
Assay ≥99.5% (GC)
Total impurities <0.1% Free glucose
Melting Point 185-187 °C (lit.)
Cation traces heavy metals (as Pb) : <5 ppm
Absorbance A50%/260 <0.15

66. 2X SYBR® Green qPCR Master Mix

The master mix should contain all of the optimized reagents and buffers needed for SYBR® Green based real-
time polymerase chain reactions in all real-time PCR instrumentation including ABI® 7500 real-time PCR
system, including real-time PCR buffer, a high-performance HotStart DNA Taq polymerase, nucleotides, and
ROX® reference dye

67. TEMED
N,N,N;N’- Tetramethylethylenediamine, C6H6N12
M = 116.21 G/MOL
Boiling point………….. 121oC
o
n 20 /C…………………. 1.417

Assay (GC)……………. .min99%

Identity ………………… complies
Water (K.F.)………….. max. 1%
TEMED should be suitable for enhancing the polymersitation (cross-linking) of acrylamide and
bisacrylamide in gel electrophoresis. It should catalyse the formation of free radicals of the initiator of
the polymerization

68. Tetracycline
Form powder
Melting point 220-223 °C (lit.)
Solubility H2O: 10 mg/mLas a stock solution. Stock solutions should be filtered sterilized Stable
at 37°C for 4 days.
Suitability Cell culture grade

69. Thiamine Hydrochloride

Formula: C12H17ClN4OS•HCl

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 Molecular Wt: 337.27
Form: Powder
Appearance: White Powder
Application: Vitamin
Solubility: Water

70. THIDIAZURON
Linear Formula C9H8N4OS
Molecular Weight 220.25
Suitability plant cell culture grade

71. Timentin
Formula C15H14N2Na2O6S2/ C8H8KNO5
Molecular Wt 428.4/ 237.3
Form Powder
Appearance Off-white to Yellow Powder
Application Plant Tissue Culture Antibiotic
Solubility Water

72. Tris base ultrapure

Assay (titr.) . . . . . . . . . . . . . min. 99.9%

pH (1 M; H2O) . . . . . . . . . . . 10.5 - 11.5 (20˚C)
Water . . . . . . . . . . . . . . . . . max. 0.2 %
Cu . . . . . . . . . . . . . . . . . . . . max. 0.0001%
Fe . . . . . . . . . . . . . . . . . . . . max. 0.0001%
Pb . . . . . . . . . . . . . . . . . max. 0.0001%

73. Tris HCL

Form crystalline
Color white
Useful pH range 7-9
pKa (25 °C) 8.1
Boiling point 219-220 °C/10 mmHg(lit.)
Melting point 167-172 °C(lit.)

DNases/RNases/
Proteases . . . . . . . . . . . not detectable
Acrylic acid (titr.) . . . . . . max. 0.001 %

74. Tryptone
Quality Microbiologically tested.
Nitrogen analysis ~13% total
Preparation Note Pancreatic digest of casein

75. X-Gal
Formula: C14H15BrClNO6
Molecular Wt: 408.63
Form: Powder
Appearance: White Powder
Application: Molecular Biology
Solubility: DMSO or KOH
76. X-Gluc, Sodium Salt
Formula: C14H12BrClNaNO7
Molecular Wt: 444.6
Form: Powder

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 Appearance: White Powder
Application: Molecular Biology
Solubility: DMSO or KOH
77. Yeast extract
Solubility H2O: 10% remains clear after heating to 40°C
Suitability Plant cell culture grade

A50%/280 <0.15
78. Zeatin
Linear Formula: C10H13N5O
Molecular Weight: 219.24
Approx. 80% Trans (Mixed Isomers)
Form powder
Color off-white to yellow
Suitability plant cell culture grade
79. Triton X-100
Linear formula t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10
mol wt avg mol wt 80,000, average mol wt 625
transition temp 65 °C
HLB 13.5
Flash Point(F) 483.8 °F
Flash Point(C) 251 °C
Biochem/physiol Actions Widely used non-ionic surfactant for recovery of membrane components under
mild non-denaturing conditions.

80. Sodium Phosphate,Monobasic Monohydrate

Formula NaH2PO4•H2O
Molecular Wt 137.99
Form Powder
Appearance: White Crystalline
Application Plant Macronutrient
Solubility Water
Suitability Plant Tissue Culture Tested
81. Hoagland Modified Basal Salt Mixture
Form Powder
Appearance White to Yellow Powder
Application Plant Tissue Culture and Hydroponic nutrient medium suitable for many plant
species
Solubility Water
Contains the macro- and micronutrients as described by Hoagland & Arnon
(1950).
82. 2X TaqMan® qPCR Master Mix
The ready to use master mix should contain all the reagents (except primers, probe, and template) needed for
running quantitative, real-time DNA-detection assays, including qPCR and two-step qRT-PCR, in the hydrolysis
probe detection format on ABI® 7500 real-time PCR system, and supplemented with ROX® passive reference dye

83. Chip kits compatible with Bioanalyzer 2100

a. RNA Nano Chip Kit

Low sample and reagent consumption

Qualitative range (total RNA) 5-500 ng/µl (rnRNA) 25-250 ng/µl
Quantitative range (total RNA) 25-500 ng/µl (rnRNA) 25-250 ng/µl
Reproducibility of quantitation 10 % CV 10 % CV

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b. DNA Chip Kit
i) Resolution and quantitation range 25–500bp
ii) Resolution and quantitation range 25–1000 bp
iii) Resolution and quantitation range 100–7500 bp

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