A Project Report On

ISOLATION AND CHARACTERIZATION OF MICROORGANISMS FROM

THE GUT OF EARTHWORM AND TO CHECK THEIR SENSITIVITY
AGAINST PESTICIDES

A DISSERTATION

SUBMITTED
IN PARTIAL FULFILLMENT OF THE REQUIREMENTS
FOR THE DEGREE OF
MASTER OF SCIENCE

IN

BIOTECHNOLOGY

SUBMITTED BY:
ABHA CHAUDHARY
ROLL NO. 1596602501
UNDER SUPERVISION OF
Dr. K.B. SINGH

INSTITUTE OF APPLIED MEDICINES & RESEARCH

CH. CHARAN SINGH UNIVERSITY, MEERUT

MEERUT-250004
 ACKNOWLEDGEMENT
I wish to thank, Mr. Rajvardhan Singh Tripathi, Dr.Neha and all the staff members of
IAMR, Ghaziabad, for his constant encouragement and co-operation.

I extend my deep sense of gratitude to Dr. K.B Singh, Director of Allele Life Sciences
Private Limited, for his gracious and inspiring guidance, keen interest and constant
encouragement and invaluable help throughout project work. I sincerely thank her for
her stoic presence, personal affection, friendly counsel and never failing support.

I am deeply indebted to Dr. Mrityunjay for his valuable guidance, constant help,
constructive criticism and providing necessary facilities for this project work.

I wish to express my heartfelt thanks to my parents, siblings who constantly motivated me

in my academic pursuit.

Abha Chaudhary
M.Sc Biotechnology
IInd Year
 DECLARATION BY THE CANDIDATE
I hereby declare that the dissertation entitled “Isolation and Characterization of
Microorganisms from the Gut of Earthworm and to check their Sensitivity against
Pesticides” embodies the work done by me at the Allele Life Science Private Limited,
Noida under the supervision of Dr. K.B. SINGH.

This work in part or full has not been submitted to any other university.

Date:
Signature of candidate.
Place: Noida
 CONTENTS
 INTRODUCTION
 REVIEW OF LITERATURE
 Earthworm
 Soil Microorganism
 Agrochemical
 MATERIALS AND METHODS
 Collection of earthworm
 Dissection of earthworm
 Media preparation
 Isolation of Microorganism
 Culturing of Microorganism
 Identification of Microorganism
 Preparation of pesticide concentration
 Well diffusion method

 RESULTS
 Isolation of Microorganism
 characterization of isolated Microorganism
 Antimicrobial effect of pesticides against different microbial
isolate
 DISCUSSION
 SUMMARY AND CONCLUSION
 REFERENCE
 APPENDICES