Standard Curve for estimation of Proline

Concentration
1.4

1.2
y = 0.0863x 1.219
Absorbance at 520 nm

0.8 0.819

0.6
0.528
0.4

0.255
0.2
0.095 0.106
0 0
0 2 4 6 8 10 12 14
Proline Concentration (μg/ml)

Standard Curve for estimation of

Carbohydrate Concentration
0.8 0.76

0.7 y = 0.01x 0.631

Absorbance at 525 nm

0.6
0.5
0.5

0.4 0.361

0.3 0.228

0.2
0.092
0.1 0.055
0
0
0 10 20 30 40 50 60 70 80

Concentration of dextrose (μg/ml)

 Standard curve for estimation of Free Amino
Acids
0.45

0.4 y = 0.0103x 0.426

Absorbance at 570 nm

0.35

0.3

0.25 0.285

0.2

0.15 0.19

0.1 0.14

0.05
0.06
0 0.035
0 5 10 15 20 25 30 35 40 45

Concentration of Proline (μg/ml)