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Protocol for the synthesis of silver nanoparticles using sodium citrate and
sodium borohydride as reducing agents
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Table of Contents
Page
Disclaimer 1
1. Introduction: Principles of synthesis and application of nanoparticles 1
2. Materials, Reagents and Equipment needed 3
3. Protocol for synthesis and results of characterization 3
4. Characterization of nanoparticles. 4
5. Acknowledgements 5
6. References 6
DISCLAIMER
The present protocol is intended for use in a safe environment, such as a laboratory, and under
the supervision of professionals. Be aware that, in spite of the simplicity of the procedures,
there is an inherent risk in the handling of chemicals and in the disposal of residues.
The matter in the nanoscale present properties which are completely different to those
observed in the macroscale (above 1 m). For example, while gold has been known to be an
inert, noble metal, in nanoparticle form it is an excellent catalyst, facilitating a broad range of
oxidation reactions.[1] Another example is that of silicon, which is a semiconductor in the
macroscale but in the nanoscale it behaves as a conductor (quantum dot).The known
antibacterial properties of silver (whose ions are able to interfere with key biological processes
in a bacteria) are enhanced when silver is used as nanoparticles, not only due to the slow
release of silver ions, but also due to the nanoparticle-mediated formation of reactive oxygen
species (ROS), which contribute to the destruction of bacteria. The more controlled, targeted
manner in which silver nanoparticles operate reduces the impact of silver in health and
aquatic life.[2] (Mavani, K .; Shah, M., 2013).
White papers in nanomaterials
2.1. Reagents
Deionized water
Silver nitrate, AgNO3(99.8% - J.A. Elmer)
Sodium citrate (99.9% -Movilab)
Sodium borohydride, NaBH4 (>98%, Sigma-Aldrich)
Sodium hydroxide (NaOH, 0.1M, Merck)
Nitric acid (HNO3, 0.1%, J.T. Baker)
4. CHARACTERIZATION
there are Ag NPs of larger sizes than at pH 9. Thus, it is clear that the optimal pH is 9 as it
provides a significant amount of Ag NPs without a broad distribution of sizes.
4.2. Citrate vs. borohydride as reducer. Figure 3 shows the difference in the UV-vis spectra
collected using borohydride and citrate. From the difference in intensities, it is clear that
borohydride produces more nanoparticles than citrate. Moreover, from the narrowness of the
borohydride spectra it is deduced that the nanoparticle size is also more uniform with
borohydride.
Fig. 4. Scanning Electron Microscopy (SEM) images: A) Activated carbon exposed to a citrate
solution (without Ag NPs); B) and C) Ag NPs supported on activated carbon with citrate as a
reducer; D) Ag NPs supported on activated carbon with borohydride as a reducer.
White papers in nanomaterials
4.3. Scanning Electron Microscopy (SEM) images. Figure 4 shows a micrograph of activated
carbon exposed to citrate solution with and without Ag NPs. The size of nanoparticles
detected is around 80 nm. Micrographs of activated carbon impregnated with Ag NPs from
borohydride solutions are not shown due to the fact that borohydride modified the
morphology of the activated carbon (it did not show a porous structure such as in Fig. 4), likely
due to the reaction of borohydride and adsorption of by-products on the surface of activated
carbon.
5. ACKNOWLEDGEMENTS
This work has been supported by the Universidad de Ingeniería y Tecnología – UTEC and
Cleveland Clinic through a research grant for joint research projects. Ms. Karinna Visurraga
(UTEC) is acknowledged for administrative and technical support. Dr. Vijay Krishna (Cleveland
Clinic), Mr. Luis Palomino and Mr. Miguel Gakiya (UTEC) are thanked for useful discussions.
6. REFERENCES
[1] T. A. Baker, X. Liu, and C. M. Friend, “The mystery of gold’s chemical activity: local bonding,
morphology and reactivity of atomic oxygen.,” Phys. Chem. Chem. Phys., vol. 13, no. 1, pp.
34–46, 2011.
[2] K. Mavani, M. Shah, “Synthesis of silver nanoparticles by using sodium borohydride as a
reducing agent”, Int. J. Eng. Res. Tech., vol. 2, no. 3, pp. 1-5, 2013.
[3] S. Solomon, M. Bahadori, A. V Jeyarajasingam, S. A. Rutkowsky, C. Boritz, and L. Mulfinger,
“Synthesis and Study of Silver Nanoparticles,” J. Chem. Educ., vol. 322, no. 2, pp. 322–325,
2007.
[4] Z. Khan, S. A. Al-Thabaiti, A. Y. Obaid, A. O. Al-Youbi, “Preparation and characterization of
silver nanoparticles by chemical reduction method”, Colloids Surf. B., vol. 82, no.2, pp. 513-
517,2011.
[5] X. Lu, M. Rycenga, S. E. Skrabalak, B. Wiley, and Y. Xia, “Chemical synthesis of novel plasmonic
nanoparticles.,” Annu. Rev. Phys. Chem., vol. 60, pp. 167–92, 2009.