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Abstract
Introduction
Some solutions absorb light at certain visible wavelengths more than others. This
property gives the solution a colour which can be observed by the eye. Copper
sulphate is blue in solution, since the Cu2+ ions absorb the red (longer wavelength)
light which pass through. The amount of light absorbed is proportional to the
concentration of the solution, and the path length through which the light passes. This
𝐴 = 𝜀 ∗ 𝑐 ∗ 𝑙
where A is the absorbance, c the concentration (in molar), l the length in cm, and ε is
wavelength, for example 600 nm for red light, and zeroed using a solution which does
not absorb at the wavelength of interest, for example water. To measure the
which the path length (l) is known precisely. The cuvettes we used were 1 cm in
length. Plastic cuvettes are OK when measuring using visible light, but for measuring
measured. A plot of absorbance vs. concentration (in molar) gives a straight line
whose equation is given by the Lambert-Beer law. The slope of the line is the molar
extinction coefficient. The reason for taking many measurements rather than just one
Methods
Materials
CuSO4 as solid matter
Distilled water
Bottles
Pipettes and pipette wheel
Cuvette
Spectrophotometer
Beakers
Test tubes
Magnetic stirrer
Glass stirrer
Analytical balance
Prepared by: Eng. Elham Ghazi Al-Hamaideh
Data sheet
Date Tasted:
Tested by:
Group Number:
Sample Number:
Sample Description:
Table
V of CuSO4 V of
Tube # V of H2O (ml) C A
(ml) solution
1
2
3
4
5
6
Sample calculations
𝑔 𝑔 𝑔
(molar mass for atoms are Cu (63.5 𝑚𝑜𝑙) , O (16 𝑚𝑜𝑙 ), S (32 𝑚𝑜𝑙)
- Concentrations ( 𝑀1 𝑉1 = 𝑀2 𝑉2)
Tube #1:
Tube #2:
Tube #3:
Tube #4:
Tube #5
Tube #6:
Prepared by: Eng. Elham Ghazi Al-Hamaideh
Questions
1. What is the definition of Beer's law? What is the importance of Beer's Law?