FILENAME: SBTY309.

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Course
BTY309
1 number
2 Course Title Basic Plant Biotechnology
3 Credits 4
Contact Hours
3-0-2
4 (L-T-P)
To introduce the topic of plant tissue culture and its industrial and agricultural application. To
develop the knowledge and techniques of production of industrial compounds. To set up
Course
appropriate conditions for regeneration of transgenic plants from genetically manipulated cells,
Objective
clonal propagation of horticultural and forest species, etc. To develop the knowledge of
5 conservation of germplasm of endangered plant species and other important plants.
After successfully completion of this course students will be able to:
1. Comprehend the basic concept of plant tissue culture and the requirements necessary for its
application.
2. Develop idea for the production of appropriate bioreactors for industrial purpose.
3. Review new and exciting developments that have taken place in the field of plant tissue
culture.
Course
6 4. Demonstrate experimentally totipotency, organogenesis and differentiation.
Outcomes
5. Apply somaclonal variation and germination techniques in regenerating recalcitrant plant
species.
6. Produce somatic and zygotic embryos and perform somatic hybridization.
7. Recognize the importance and utility of germplasm conservation.
8. Improve the characters of crop plants using micropropagation techniques.
9. Develop new horticultural species by clonal propagation.
7 Outline syllabus:
7.01 XXXNNN.A Unit A Introduction to Plant biotechnology
History of plant tissue culture and composition of tissue culture media and
7.02 XXXNNN.A1 Unit A Topic 1 growth hormones
Callus Initiation, maintenance and subculture techniques, suspension culture,
7.03 XXXNNN.A2 Unit A Topic 2 single cell culture techniques
7.04 XXXNNN.A3 Unit A Topic 3 Bioreactors, designing and types of reactors
7.05 XXXNNN.B Unit B Cytodifferentiaton
Cellular totipotency, Organogenic differentiation, Factors affecting
7.06 XXXNNN.B1 Unit B Topic 1 organogenesis, Advantages and disadvantages
7.07 XXXNNN.B2 Unit B Topic 2 Somaclonal variation techniques: Advantages and disadvantages
7.08 XXXNNN.B3 Unit B Topic 3 Techniques used for germination, pollination and cryopreservation
7.09 XXXNNN.C Unit C Somatic embryogenesis and Fertilization
Production of somatic embryos, Factors affecting Somatic embryogenesis,
7.10 XXXNNN.C1 Unit C Topic 1 Somatic embryos and zygotic embryos
7.11 XXXNNN.C2 Unit C Topic 2 Large scale production of somatic embryos, Somatic hybridization
7.12 XXXNNN.C3 Unit C Topic 3 Haploid plants: their utility and application
7.13 XXXNNN.D Unit D Protoplast Culture
7.14 XXXNNN.D1 Unit D Topic 1 Isolation of protoplast
7.15 XXXNNN.D2 Unit D Topic 2 Factors responsible for viability
7.16 XXXNNN.D3 Unit D Topic 3 Protoplast fusion
7.17 XXXNNN.E Unit E Clonal Propagation
7.18 XXXNNN.E1 Unit E Topic 1 Cybridization
7.19 XXXNNN.E2 Unit E Topic 2 Micropropagation techniques and its application in industry
7.20 XXXNNN.E3 Unit E Topic 3 Clonal propagation, factors responsible and advantages
7.21 XXXNNN.L01 Lab Expt 1 Sterilization of working place, glass wares , etc
7.22 XXXNNN.L02 Lab Expt 2 Plugging of culture & test tube
7.23 XXXNNN.L03 Lab Expt 3 Media preparation, preparation of stock solution, media autoclaving.
7.24 XXXNNN.L04 Lab Expt 4 Inoculation of explants under Laminar flow and uses of Growth Chamber
7.25 XXXNNN.L05 Lab Expt 5 Subculture techniques
7.26 XXXNNN.L06 Lab Expt 6 Preparation of Liquid culture and single cell culture
7.27 XXXNNN.L07 Lab Expt 7 Development of homogenous colony from single cell culture
7.28 XXXNNN.L08 Lab Expt 8 In vitro micropropagation
8 Course Evaluation
8.1 Course work: 30 marks
8.11 Attendance None
8.12 Homework None
8.13 Quizzes Two 30-minutes surprise quizzes in lecture hours: 10 marks
Evaluation of work done on each lab turn in the lab notebook and feedback from oral quiz about
the work done that day. Zero, if the student is absent. 0.75N best marks out of N such evaluations:
8.14 Projects 20 marks
8.15 Presentations None
8.16 Any other None
8.2 MTE One, 20 percent
8.3 End-term examination: 50 percent
9 References
Stewart C.N., “Plant Biotechnology and Genetics: Techniques and Applications”, Wiley-
9.1 Text book Interscience’ 2008.
Other 1. Bhojwani S.S., Dantu P.K., “Plant Tissue Culture: An Introductory Text”, Springer, 2013.
9.2 References 1. Oksman-Caldentey K-M., “Plant Biotechnology and Transgenic Plants; CRC Press, 2002.

Mapping of Outcomes vs. Topics

Outcome no. → 1 2 3 4 5 6 7 8 9
Syllabus topic ↓
XXXNNN.A X X X
XXXNNN.A1 X X
XXXNNN.A2 X X
XXXNNN.A3 X
XXXNNN.B X X
XXXNNN.B1 X
XXXNNN.B2 X
XXXNNN.B3 X
XXXNNN.C X
XXXNNN.C1 X
XXXNNN.C2 X
XXXNNN.C3 X
XXXNNN.D X
XXXNNN.D1 X
XXXNNN.D2 X
XXXNNN.D3 X
XXXNNN.E X X
XXXNNN.E1 X
XXXNNN.E2 X
XXXNNN.E3 X