Aquarius® Prenatal Probe Range Cytocell's prenatal Fluorescence in Situ Hybridisation (FISH) assays are designed for the rapid and accurate detection of the ‘most common foetal chromosomal disorders. ‘Tiisomy of chromosome 21, resulting in Down syndrome’, is one of the most common chromosome abnormalities in humans and the risk of having an affected child is known to increase with maternal age®, The syndrome represents a particular ‘combination of phenotypic findings, including characteristic facial appearance, a single palmar crease and mental retardation, ‘and may also present with hearing and heart defects. Affected individuals show a highly increased incidence of leukaemia, particularly acute megakaryocytic leukaemia’. “Tisomy of chromosome 18, resulting in Edwards syndrome, occurs in around 1 in 6000-8000 live births with a female sex bias*. The clinica findings are variable, though many exhibit growth delay, heart defects and craniofacial anomalies, as well as possible limb and kidney abnormaliies*. ‘The rarest trisomy, trisomy 13, responsible for Patau syndrome occurs in approximately 1 in 16,000 newborns’. individuals ‘with Patau syndrome present abnormalities affecting many parts of the body, including the heart, spinal cord, eyes, limbs, face/skull and muscles’ ‘Aberrant copy numbers of the X and Y chromosomes can lead to various sex chromosome disorders, such as Kiinefelter (47,XXY), Tuer (45,X) and other syndromes caused by variations in copy number of X and/or Y. These syndromes have variable incidences and clinical findings’. Cytocell Prenatal kts contain fluorescent probes for easy identification of trisomies 21, 18 and 13 present in Down, Edwards ‘and Patau syndrome, respectively, as well as multiple sex chromosome aneuploidies.. Aquarius® FAST FISH Prenatal Kits “The Aquarius” FAST FISH Prenatal kit allows detection of trisomies 13, 18 and 21 (Down syndrome) and sex chromosome aneuploigies utlsing a 2 hour hybridisation protocol. ‘Aquarius® FAST FISH Pronatal kits provide the benafits of: + 2 hour hybridisation protocol demonstrating high intensity signals and minimal background, + Economical kit formats: §, 10, 30° or 0" tests. + Liquid stable reagents premixed in hybridisation solution and provided with DAPI counterstain Each kt contains the variable probe sets, detalls of which are listed below. soar Ca aE X centromere Xpt11-q11.1 (0X21) Green Y centromere Ypt1.1-q11.1 (OYZ3) Orange 18 centromere 18p11.1-q11.1 (01821) Blue Probe set wan 19 unique sequence (184142) Green 21 unique sequence (21q22.13) Orange a LPF 002 X centromere Xpt1-1-q11.1 (OXZ!} Green Y centromere Yptt-1-q11.1 (OYZ3) Orange 18 centromere 18p11.1-q11.1 (01821) Blue 1 unique sequence (13q14.2) Green Bonen rou pscm otic 21 unique sequence (2192.19) OrangeAquarius® Prenatal Enumeration Kits ‘The Aquarius® Prenatal Enumeration range allows detection of trisomies 13, 18 and 21 (Down syndrome) and sex chromosome, aneupioicies utlsing an overnight hybridisation protocol, ‘Aquarius® Prenatal kis provide the benefits of: + Overnight hybridisation protocol demonstrating high intensity signals and minimal background. + Economical kt formats: 5, 10, 30" or 50" tests. + Liquid stable reagents premixed in hybridisation solution and provided with DAPI counterstain. Each kt contains the folowing probe sets. Probe set xz X centromere Xp11.1-q11.1 (DXZ1) Green YY centromere Yp11.1-q11.1 (DYZ3) Orange 18 centromere 18p11.1-q1 1 (01821) Blue — Probe set oi ‘Sure sequence (19142) Geen 21 une saioce (212.19 Orange ee X centromere Xp11.1-q11.1 (OXZ1) Green YY centromere Yp11.1-q11.1 (DYZ3) Orange 18 centromere 18911.1-q11.1 (D182!) Blue 19 unique sequence (13414.2) Green 21 unique sequence (21q22.13) Orange —<—<— << 18 centromere 18p11.1-q1 1.1 (D18Z1) Blue 418 unique sequence (13414.2) Green 1B centromere 18p11.1-q11.1 (D18Z1) Blue 21 unique sequence (21q22.13) Orange Sane ae ios rtelanmascontar aac tepuennam gooeeatontann 1 | Sesweammarenn ans SeSmanicsdacy Gone reeset 7st Fema rn Span Sr Rr Co 205 2