Barcoded Reverse Primers

Name Barcode Index read Sequence (5’-3’)

R index 2 ACATCG CGATGT CAAGCAGAAGACGGCATACGAGATACATCGGTGACTGGAGTTCAGACGTGTGCTCT
TCCGATCTACTTGCTATTTCTAGCTCTAAAAC
R index 5 CACTGT ACAGTG CAAGCAGAAGACGGCATACGAGATCACTGTGTGACTGGAGTTCAGACGTGTGCTCT
TCCGATCTACTTGCTATTTCTAGCTCTAAAAC
R index 6 ATTGGC GGCAAT CAAGCAGAAGACGGCATACGAGATATTGGCGTGACTGGAGTTCAGACGTGTGCTC
TTCCGATCTACTTGCTATTTCTAGCTCTAAAAC
R index 8 TCAAGT ACTTGA CAAGCAGAAGACGGCATACGAGATTCAAGTGTGACTGGAGTTCAGACGTGTGCTCT
TCCGATCTACTTGCTATTTCTAGCTCTAAAAC
R index 3 GCCTAA TTAGGC CAAGCAGAAGACGGCATACGAGATGCCTAAGTGACTGGAGTTCAGACGTGTGCTCT
TCCGATCTACTTGCTATTTCTAGCTCTAAAAC
R index 4 TGGTCA TGACCA CAAGCAGAAGACGGCATACGAGATTGGTCAGTGACTGGAGTTCAGACGTGTGCTC
TTCCGATCTACTTGCTATTTCTAGCTCTAAAAC
R index 7 GATCTG CAGATC CAAGCAGAAGACGGCATACGAGATGATCTGGTGACTGGAGTTCAGACGTGTGCTC
TTCCGATCTACTTGCTATTTCTAGCTCTAAAAC
R index 10 AAGCTA TAGCTT CAAGCAGAAGACGGCATACGAGATAAGCTAGTGACTGGAGTTCAGACGTGTGCTC
TTCCGATCTACTTGCTATTTCTAGCTCTAAAAC

Universal Forward Primers

Name Stagger Sequence (5’-3’)
F universal (0nt) - AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT
TTGTGGAAAGGACGAGGTACCG
F universal (1nt) C AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT
CTTGTGGAAAGGACGAGGTACCG
F universal (2nt) GC AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT
GCTTGTGGAAAGGACGAGGTACCG
F universal (3nt) AGC AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT
AGCTTGTGGAAAGGACGAGGTACCG
F universal (4nt) CAAC AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT
CAACTTGTGGAAAGGACGAGGTACCG
F universal (6nt) TGCACC AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT
TGCACCTTGTGGAAAGGACGAGGTACCG
F universal (7nt) ACGCAAC AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT
ACGCAACTTGTGGAAAGGACGAGGTACCG
F universal (8nt) GAAGACCC AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT
GAAGACCCTTGTGGAAAGGACGAGGTACCG