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Full Title: FIELD RESISTANCE OF NEW PROMISING SUGARCANE CLONES AGAINST TOP
BORER AND SUGARCANE MOSAIC VIRUS IN INDONESIA
Funding Information:
Abstract: Sugarcane (Saccharum officinarum L.) possesses a high economic value since it
serves as a basic material for the production of sugar. Sugar production in Indonesia
faces several major obstacles, one of which is infestation from pests and disease
especially top borer and mosaic virus. This research aimed to identify the resistance of
new sugarcane clones to top borer and mosaic virus so that they can be utilized to
develop top borer and mosaic virus -resistant sugarcane and to improve productivity.
To test the field resistance of new promising sugarcane clones, an experiment was
conducted in Ngemplak Experimental Station. Fifty-nine potential clones with one
controlling variety (PS-921 variety or Kentung) were tested for their relative degree of
tolerance and susceptibility against top borers infestation and mosaic virus incidence.
These sugarcane clones were grown in Randomized Block Design with three
replications. Each variety was planted in a plot consisting of 3 rows @ 5-meter length.
The seedling was planted 50 cm apart so that the number of plants per plot was 30
plants. The distance of PKP (from center to center) was 100 cm, while the distance
between replicates was 3m. The observation included the types and percentage of top
borer infestation and mosaic virus incidence at two weeks interval. The results showed
that some of the new sugarcane clones were tolerant to top borer infestation and
mosaic incidence. The lowest sugarcane top borer infestation (tolerant category) was
found in five sugarcane clones, i.e. PS.06.334, PS 04.257, PS 06.356, PS 06.381 and
PS 04.259. Meanwhile, the lowest sugarcane mosaic virus incidence (tolerant
category) was found in one sugarcane clones, i.e. PS.05.258. programs. The top borer
infestation and mosaic virus incidence negatively affected the height of the sugarcane
plant, the length of the processed stalk, the weight of the stalk, yield, production, and
the sugar content. The resistant clones identified in this study could be used as
sources of top borer and mosaic virus resistance in sugarcane breeding.
Corresponding Author's Institution: Indonesian Sweetener and Fiber Crops Research Institute
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Assist. Professor, Cairo University
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Cover Letter Click here to access/download;Cover Letter;COVERING
LETTER sugarcane.docx
TITLE PAGE
Heri Prabowo1a, Nur Asbani1b, Supriyadi1c, Bambang Heliyanto1d, and Sri Adikadarsih1e
1
Indonesian Sweetener and Fiber Crops Research Institute
Jl Raya Karangploso Kotak Pos 199, Malang, East Java, Indonesia 65152
Correspondence : aheribalittas@gmail.com, bnurasbani@yahoo.com, csupriyadi@yahoo.com,
d
b.heliyanto@yahoo.com and eadikadarsih@yahoo.com
COVERING LETTER
I am pleased to submit an original research article entitled “Field resistance of new promising
sugarcane clones against top borer and sugarcane mosaic virus in Indonesia” by Heri Prabowo, Nur Asbani,
Supriyadi, Bambang Heliyanto and Sri Adikadarsih for consideration for publication in Egyptian
Journal of Biological Pest control. We are all researcher from Indonesian sweetener and fiber crops
research institute with address Jl Raya Karangploso Kotak Pos 199, Malang, East Java, Indonesia 65152.
Details contact all author i.e. Heri Prabowo (heribalittas@gmail.com), Nur Asbani
(nurasbani@yahoo.com), Supriyadi (supriyadi@yahoo.com), Bambang Heliyanto
(b.heliyanto@yahoo.com) and Sri Adikadarsih (adikadarsih@yahoo.com).
In this manuscript, we show that the resistant sugarcane clones identified in this study could be
used as sources of top borer and mosaic virus resistance in sugarcane breeding. The five sugarcane clones
PS.06.334, PS 04.257, PS 06.356, PS 06.381 and PS 04.259 could be uses as sugarcane clones resistance
to control top borer infestation. Meanwhile, for controlling mosaic virus, we can use sugarcane clone
PS.05.258.
This manuscript has not been published and is not under consideration for publication elsewhere. We
have no conflicts of interest to disclose. The second author, third author, fourth writer, and fifth author agreed to
publish the manuscript. All authors read and approved the final manuscript. We clarify that figures or tables on this
paper have not previously been published elsewhere.
Sincerely,
Heri Prabowo
1 Table 1. Potential high yield clones and control varieties which evaluated on this research
1
12 PS.06.181 13,13defghij Susceptible 42 PS.06.188 6,94efghij Moderately tolerant
2
16.67
15 PS.06.369 45.83cdefghij Higly susceptible 45 PS.05.124 Susceptible
abcdefg
16.67
16 PS.04.237 43.06bcdefghij Higly susceptible 46 PS.06.303 Susceptible
abcdefg
15.28
17 PS.06.181 41.67abcdefghij Higly susceptible 47 PS.04.392 Susceptible
abcdef
15.28
18 PS.05.130 41.67 ab Higly susceptible 48 PS.05.390 Susceptible
abcdef
19 PS.06.391 38.89 abcdefghij Higly susceptible 49 PS.05.393 13.89abcde Susceptible
26 PS.05.455 33.33 abcdefghi Higly susceptible 56 PS.05.251 9.72 abcd Moderately tolerant
27 kentung 31.94 abcdefgh Higly susceptible 57 PS.05.327 9.72 abcd Moderately tolerant
28 PS.06.103 31.94 abcdefgh Higly susceptible 58 PS.04.380 8.33 abcd Moderately tolerant
29 PS.06.400 31.94 abcdefgh Higly susceptible 59 PS.06.395 8.33 abc Moderately tolerant
3
b
Figure Click here to access/download;Figure;figure.docx
25
15
10
0
2 4 6 8 10 12 14 16 18 20 22 24 26
300 a ab b b
250
the height of the plant (cm)
200
150
243,8 260,6
100 255,0 258,7
50
0
Highly Susceptible Moderately Tolerant
susceptible tolerant
resistance criteria
5 Fig 2. Relationship between top borer resistance criteria and the height of the plant. Bars represent the standard errors of the means.
6 Means sharing similar letters for each location are insignificantly different at (P > 0.05), using DMRT Test.
7
ab b
300 a ab
the length of the processed stalk
250
200
150 252,9 258,5 265,5
235,1
(cm)
100
50
0
Highly Susceptible Moderately Tolerant
susceptible tolerant
resistance criteria
8 Fig 3. Relationship between top borer resistance criteria and the length of the processed stalk. Bars represent the standard errors of the means.
9 Means sharing similar letters for each location are insignificantly different at (P > 0.05), using DMRT Test.
10
11
1
b
2500 b
ab
1500
1000
1492,8 1740,8 1915,7 2120,1
500
0
Highly Susceptible Moderately Tolerant
susceptible tolerant
resistance criteria
12 Fig 4. Relationship between top borer resistance criteria and the weight of the stalk. Bars represent the standard errors of the means. Means
13 sharing similar letters for each location are insignificantly different at (P > 0.05), using DMRT Test.
14
a ab b b
10
8
cane sugar (%)
Commercial
6
4 8.7 8,9 8,9 9
2
0
Highly Susceptible Moderately Tolerant
susceptible tolerant
resistance criteria
15
16
17 Fig 5. Relationship between top borer resistance criteria and the sugar content. Bars represent the standard errors of the means. Means
18 sharing similar letters for each location are insignificantly different at (P > 0.05), using DMRT Test.
19
b c c
the height of the plant (cm)
350 a
300
250
200
150
290,0 297,2 299,5 299,5
100
50
0
Highly Susceptible Moderately Tolerant
susceptible tolerant
resistance criteria
20 Fig 6. Relationship between mosaic virus resistance criteria and the height of the plant. Bars represent the standard errors of the means.
21 Means sharing similar letters for each location are insignificantly different at (P > 0.05), using DMRT Test.
22
23
2
the length of the processed stalk (cm)
300 b c d
a
250
200
150
100 243,8 255,0 258,7 260,6
50
0
Highly Susceptible Moderately Tolerant
susceptible tolerant
resistance criteria
24 Fig 7. Relationship between mosaic virus resistance criteria and the length of the processed stalk. Bars represent the standard errors of the
25 means. Means sharing similar letters for each location are insignificantly different at (P > 0.05), using DMRT Test.
26
27
the weight of the stalk (gr)
3000 d
c
2500 b
a
2000
1500
1000
500 1755,9 1767,0 2568,9 2420,6
0
Highly Susceptible Moderately Tolerant
susceptible tolerant
resistance criteria
28 Fig 8. Relationship between mosaic virus resistance criteria and the weight of the stalk. Bars represent the standard errors of the means.
29 Means sharing similar letters for each location are insignificantly different at (P > 0.05), using DMRT Test.
30
31
12 d
a b c
10
the sugar content (%)
8
6
4
8.7 8,8 8,9 9.4
2
0
Highly Susceptible Moderately Tolerant
susceptible tolerant
resistance criteria
32 Fig 9. Relationship between mosaic virus resistance criteria and the sugar content. Bars represent the standard errors of the means. Means
33 sharing similar letters for each location are insignificantly different at (P > 0.05), using DMRT Test.
34
35