REFERENCE:

1. Dugas, Hermann “ Bioorganic Chemistry: A Chemical Approach to Enzyme Action” 3rd

Edition, Springer, 2003.

BT8305 CELL BIOLOGY LTPC

3 003
OBJECTIVES:
 To provide knowledge on the fundamentals of cell biology
 To help students understand the signalling mechanisms

UNIT I CELL STRUCTURE AND FUNCTION OF THE ORGANELLES 9

Prokaryotic, Eukaryotic cells, Sub-cellular organelles and functions. Principles of membrane
organization membrane proteins, cytoskeletal proteins. Extra cellular matrix, cell-cell junctions.

UNIT II CELL DIVISION, CANCER, APOPTOSIS AND IMMORTALIZATION OF CELLS 9

Cell cycle – Mitosis, Meiosis, Molecules controlling cell cycle, cancer, role of Ras and Raf in
oncogenesis and apoptosis. Stem cells, Cell culture and immortalization of cells and its
applications.

UNIT III TRANSPORT ACROSS CELL MEMBRANE 9

Passive and Active Transport, Permeases, Ion channels, ATP pumps. Na+ / K+ / Ca+2Tpumps,
uniport, symport antiporter system. Ligand gated / voltage gated channels, Agonistsand
Antagonists.

UNIT IV SIGNAL TRANSDUCTION 9

Receptors – extracellular signaling, Cell surface / cytosolic receptors and examples,
Differentclasses of receptors antocrine / paracrine / endocrine models, Secondary messengers
molecules.
UNIT V TECHNIQUES USED TO STUDY CELLS 9
Cell fractionation and flow cytometry, Morphology and identification of cells using microscopic
studies like SEM, TEM and Confocal Microscopy. Localization of proteins in cells –
Immunostaining.
TOTAL: 45 PERIODS
OUTCOMES:
Upon completion of this course, the students
 Would have deeper understanding of cell at structural and functional level.
 Would have broad knowledge on the molecular interaction between cells.
 Would demonstrate a clear understanding of the signal transduction, secondary
 messengers.
 Would develop skill on working principles of microscopy and identification of cell types.
TEXT BOOKS:
1. Lodish, Harvey etal., “Molecular Cell Biology”, 7th Edition, W.H.Freeman, 2005.
2. Cooper, G.M. and R.E. Hansman “The Cell: A Molecular Approach”, VIIth Edition, ASM
Press, 2007.
3. Alberts, Bruce etal., “Molecular Biology of the Cell”, IVth Edition, Garland Science (Taylors
Francis), 2002.

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