Professional Documents
Culture Documents
Biotechnology
10 9 8 7 6 5 4 3 2 1
Keywords
amino acid; clotting factor; CRISPR; EPO; eugenics; GMO; h
emoglobin;
hybrid; mitochondria; mRNA; onco mouse; patents; protein; ribosome;
survival of the fittest; transcription; translation
Contents
Preface��������������������������������������������������������������������������������������������������ix
The Basics����������������������������������������������������������������������������������������������xi
Chapter 1 Introduction��������������������������������������������������������������������1
Chapter 2 How Is Biotechnology Used?�������������������������������������������7
Chapter 3 A New Breakthrough: CRISPR�������������������������������������13
Chapter 4 Genetic Engineering in the Media���������������������������������15
Chapter 5 GM Technology: What Are Pros and Cons?������������������17
Chapter 6 Patenting Genes������������������������������������������������������������21
Chapter 7 Other Cases������������������������������������������������������������������25
Chapter 8 Ethical Issues: Who Will Decide?����������������������������������27
Chapter 9 How Will This Influx of Genetic Information Affect
Our Future?������������������������������������������������������������������29
Chapter 10 The Papaya and the Biologist: The Man Behind
the Rainbow������������������������������������������������������������������31
Chromosome
Are made up of the DNA
Genes
are lined along the DNA and code for
Amino Acids
The building blocks of proteins
Introduction
First, biotechnology is a medical word. The first thing you do if you want
to discover the meaning of a medical word is separate it into its parts.
Luckily, our word has only three parts and you already know two of them:
Let us try: Bio---Techno---Ology
We all know the meaning of first syllable, Bio. It means life.
The last syllable, Ology means study of.
The middle part is a little harder; it has many meanings: Techno
generally means applying scientific knowledge for practical purposes
(medicine, farming, art, etc.).
When we define all the parts ----voila!, a meaning appears (well maybe
in your brain?).
Our word, biotechnology, means a study of how to apply technology
to living systems (see Figure 1.1).
The manipulation of small particles (such as DNA) has been available
for years. The fantastic microscopes and even more fantastic biologists
have opened our eyes to some amazing things.
So, here we go! First, go back to the page before Chapter 1 called—
The basics. This simply shows the mechanism of protein formation. We
will discuss this later as we go deeper into the DNA molecule.
What Does DNA do?
DNA has two main functions in living things:
The protein copies and DNA copies must be 100 percent perfect.
What if they are not perfect?
2 Biotechnology
Biotechnology
Genetic
engineering
Genes are
inserted or Gene inserted
turned on in bacteria
or off
If inserted
Humalin* Human
they can
human insulin clotting factor
come from
Diabetes
Same type of
Another
animal or Hemophilia
plant species
Example: Example:
Turn off a Place the
gene to make a human insulin
plant immune gene in a
to a virus bacteria.
Steps of Replication
First: The helical DNA (see Figure 1.2) splits into two strands.
Second: Each half finds the matching triplset to its pairs, from the
fluid within the nucleus and makes a clone of itself.
Introduction 3
5’ 3’
5’
G C G C
T A T A
A T A T
A T
A
C G Sugar-phosphate G C
backbone
G C C G
G C C G
T A A
C G C G
A T A T
5’
Figure 1.2 (a) Flat DNA. (b) Helical DNA (double helix)
DNA contains the code for all amino acids needed to create all human
proteins (proteins can have as many as 1,000 amino acids).
Protein synthesis: Follow along with Figure 1.3.
The mRNA moves to the ribosome that reads three bases (triplet) at a
time and presents the amino acids in order.
A mRNA is produced and takes the code, out of the nucleus into the
cytoplasm where a specific protein can be made.
This is called translation (translating a code hidden in the messenger
DNA).
Ribosomes read the code and place the correct amino acids in order.
An example of a few codes:
But what if we could cut the “wrong gene” and replace it with a
healthy one?