3 th – 4 th Septem ber 2018

Sw iss B ell H otel, Jl. T im or R aya

K upang, E ast N usa T enggara, Indonesia 85227

SC H E D U L E

D ay 1:
A genda V enue
Sept 3, 2018
08.00 - 09.00 R egistration Tilong R oom
O pening Program
O fficiating G uest of H onor:
1) D rs.Jefrry R iw o K ore, C ity
09.00 – 10.15 M ayor Tilong R oom
2) Prof.O ck y K arna R adjasa,
M .Sc., Ph.D M inistry of
R esearch, Technology and
H igher Education, Indonesia
10.15 – 10.30 C offee B reak Tilong R oom
K eynote Speaker:
D r. H ilconida P. C alum pong,
m em ber of W orld O cean
10.30 – 11.30 A ssessm ent, U nited N ations; Tilong R oom
D irector of Institute of
Environm ental and M arine
Science, Sillim an U niversity,
Philippines
Invited Speaker
11.30 – 12.00 D r. R .K . Trived y, Editor of Tilong R oom
Ecology, Environm ent and
C onservation Journal, India
12.00 – 12.30 Lunch D ining H all
1
 Camplong,
12.30 – 15.00 Paper Presentation Kolbano,
Nembrala
15.00 – 15.15 Coffee Break Dining Hall
Camplong,
15.15 – 15.45 Paper Presentation Kolbano,
Nembrala
Keynote Speaker:
Prof. Colin Binns MBBA, MPH.,
16.00 – 17.00 Ph.DFRACGP. FAFOM., Tilong Room
FAFPHM; Curtin University
Australia

2
 OPENING PROGRAM

Prayer
Pdt. Dr. Adriana Dukabain-Tunliu

Indonesia National Anthem

Words of Welcome
Frankie J. Salean, SE., MP
AWCU, Rector

Keynote Speakers:
Prof. Ocky Karna Radjasa, M.Sc., Ph.D
Ministry of Research, Technology and Higher Education, Indonesia

Dr. Hilconida P. Calumpong,

Member of World Ocean Assessment, United Nations
Director of Institute of Environmental and Marine Science, Silliman University, Philippines

Prof. Colin Binns, MBBA, MPH., Ph.DFRACGP. FAFOM., FAFPHM

Curtin University Australia

Invited Speaker
Dr. R.K. Trivedy,
Editor of Ecology Environment and Conservation Journal, India

Paper Presentation

3
MESSAGE FROM RECTOR
of Artha Wacana Christian University

Dear Authors and esteemed Readers,

It is with deep privilege I write this
Foreword to the Program Book of the 1st
International Conference on Climate Change,
Biodiversity, Food Security and Local
Knowledge (ICCC) held in Kupang, West Timor, Indonesia,
September 3-4, 2018.
ICCC begins to bring tradition of meeting of researchers,
professionals, academics, decision makers of all over the world,
experts in climate changes impacting biodiversity and food security
and those who practice local knowledge in anticipating climate
changes and mitigating the impacts.
This 1st international conference as a pioneer to particularly
encourage the interaction of research students and developing
academics with the more established academic community, to present
and to discuss new and current work in the areas related and this will
be followed up with South-Southeast Consortium meeting as part of
this conference. I also look forwards the next year joint-conference by
this consortium university members. The authors contributions will
significantly help to make this year Conference as outstanding as it is
expected. The papers contribute the most recent scientific knowledge
known in the field of the four main areas but not limited to
Environment, Nutrition, applied Technology and Policy in Marine and
Fisheries sectors.
In addition to the contributed papers, three keynote speakers
presentations are given: by Prof. Colin Binns, MBBS., MPH., PhD.,
FRACGP., FAFOM., FAFPHM., of Curtin University, Australia,
Hilconida P.Calumpong, PhD., the Director of Institute of
Environmental and Marine Science of Silliman University,
Phillippines, and Prof. Ocky K. Radjasa, MSc., PhD., Ministry of
Research, Technology, and Higher Education, Indonesia, who speaks

4
 about. To the list, one invited speaker, Prof. (Dr.) R. K.Trivedy,
the Editor of Ecology, Environment and Conservation Journal, India.
These Proceedings will furnish the scientists of the world with an
excellent reference book. I trust also that this will be an impetus to
stimulate further study and research in all these areas.
We thank all authors and participants for their contributions and
also look for further and continuous scientific works.

SIGNED
Frankie Jan Salean, SE., MP

5
PARALLEL SESSION A – Climate Change
Camplong Room

12.30 – 12.45 Effect of climate change, land uses and geology on soil organic
matter and carbon sequestration in dryland, kupang regency- east
nusa tenggara
Melinda R.S. Moata, Aydamel Takalapeta, Yofris Puay, Charles
Tlonaen

12.45 – 13.00 Climate change adaptation strategies through development of

alternative livelihoods in order to improve traditional fishermen
household income in the border area of belu regency
Chaterina A. Paulus, M.R Pellokila, Y.U.L Sobang, E.
Azmanajaya

13.00 – 13.15 Climate change adaptation in water supply sector through

innovation technology of water treatment plant in 1ndonesia:
numerical and experimental investigation of filter media in
direct filtration
Emil Azmanajaya, Chaterina A. Paulus, Maria R. Naguit,
Wilson L. Tisera

13.15 -13.30 Ecology status of mangrove in Rote-Ndao Regency

Donny M. Bessie, Wilson L. Tisera, Alfred G. O. Kase, Fanny I.
Ginzel

13.30 – 13.45 Mangrove forest habitat conditions in tanah merah coastal beach
East Nusa Tenggara
Jeriels Matatula, Meilyn Renny Pathibang, Ni Kade Ayu Dewi
Aryani, Alfred Umbu Kuala Ngaji

13.45 – 14.00 Analysis of harmful algal blooms (hab’s) phytoplankton

concentration in coastal areas of Kupang Bay East Nusa
Tenggara
Welem Yupiter Giri, Yohanes Merryanto S, Rockie R. L. Supit

14.00 – 14.15 Harvest control rules analysis of some important economic

fishes in east flores regency, east nusa tenggara province
Wilson L. Tisera, Saraswati Adityarini, Fanny I. Ginzel,
Lebrina I. Boikh

14.15 – 14.30 Prospect for transboundary small pelagic fisheries management

of Indonesia - Timor Leste in Ombai Strait
Beatrix M. Rehatta, M.M. Kamal, M. Boer, A. Fahrudin,
Zairion, Yohanes Merryanto.S

6
14.30 – 14.45 The growth of Kappaphycus alvarezii doty in different depth in Batubao
marine waters Tesabela village, West Kupang
Alfred G. O. Kase, Daud Yanti, Marniawati Kalorang, Jhon Septin M.
Siregar, Lebrina I. Boikh, Rockie R. L. Supit, Dessy Mantiri

15.00 – 15.15 Coffee Break

15.15 – 15.30 Escherichia coli quality on tilapia fish (oreocromis niloticus) cultured in
lake and river Tondano
O. Ningsih, F.G. Ijong, S. Berhimpon

15.30 – 15.45 The antitumor activity on Halimeda opuntia, Halymenia durvillaei, Padina
australis and Laurencia pappilosa Marine Algae from Nain Island
OceanNorth Minahasa Regency North Sulawesi
Kurniati Kemer, Esther D. Angkouw, Khristin I.F. Kondoy, Desy M.H.
Mantiri

7
PARALLEL SESSION B – Food Security
Kolbano Room

12.30 – 12.45 The Supplementation Effect Of Probio Fmplus As Symbiotic In Liquid And
Solid Forms On The Broiler Carcass Quality
Helda A. Y. Ninu, Catootjie L. Nalle

12.45 – 13.00 The Impact of Nutrition Training On Mothers’ knowledge And Behaviour
In Gangga Island, Indonesia
Laurensi Meity Sasube

13.00 – 13.15 Application of the marine fish scale derived nanochitosan for increasing
the food security of fishery products
Rumengan Inneke F.M., Pipih Suptijah, Stenly Wullur, Aldian Hein
Luntungan, Fallen Billy Sandana, Netty Salindeho

13.15 -13.30 Subjective Quality of Bakso Tenggiri Fish And Kakap Captural Results In
Larantuka District East Flores
Yosephina M. J. Batafor, Gregorius Gehi Batafor

13.30 – 13.45 Security Assessment and Acceptance Levels on Se'i Tuna Products
Processed with Different Material and Concentration of Liquid Smoke
Ayub U.I. Meko, Melkianus Nuhamara, Mada M. Lakapu

13.45 – 14.00 Food security analysis based on local wisdom on inland Boti tribe in
district Kie, South Central Timor Regency
Jollyanes Petrecia Ledo, Lebrina Ivantry Boikh, Sri Fatmah Sari

14.00 – 14.15 Growth analysis of red algae Aucheuma denticulatum (burman) collins &
hervey, farmed using different method and different strains in Nitbaun
village, Semau Island
Wilson L. Tisera, Chaterina Paulus, Ambariyanto

14.15 – 14.30 Production of Renewable Fuel through Decarboxylation of Pongamia

pinnata L. Seed oils
Neonufa G. F, Prakoso T, Neonufa N. E. M

14.30 – 14.45 Food water total factor productivity growth towards food security in West
Timor
Jonathan E. Koehuan, Bambang Suharto, Gunomo Djoyowasito, Liliya
D. Susanawati

14.45 – 15.00 Analysis of Diversity Seagrasses in Sikka Island, Kabola, Sub District
Alor
Jahved F.Maro, Erin A. Dollu, Adriana Blegur

8
15.00 – 15.15 Fuzzy Set Application Of Environmental Suitability Index For Rice Plant
(Oryza Sativa L) Based A Geographic Information System To Support
Sustainable Land Use Planning
Widiastuti Tiwuk, Mauboy Rony Stenly

15.15 – 15.30 Coffee Break

9
PARALLEL SESSION C – Biodiversity
Tilong Room

12.30 – 12.45 Biomass estimates and species diversity of natural and planted mangrove
forests in Trang, Thailand
Pablina Cadiz, Ponlachart Chotikarn

12.45 – 13.00 Modelling and Prediction of Fish Catch Landing in Songkhla Lagoon,
Thailand Using Artificial Neural Network, Multiple Adaptive Regression
Spline And K-Nearest Neighbors Models
Ha Thi Thu Hue, Siriporn Pradit, Apiradee Lim, Thongchai Nitiratsuwan

13.00 – 13.15 Echiuran density and distribution in relation to tidal flow and sediment
organic matter content and grain size composition
Clarissa T. Reboton, Janet S. Estacion

13.15 -13.30 A Case of Fatal Attraction: Corallivory by the Violet Coral Shell,
Coralliophila violacea on Branching Porites cylindrica
Clarissa T. Reboton

13.30 – 13.45 Improve The Capability of Testing of Export Fishery Products With Smart
Computer Systems
Sebastianus A. S. Mola

13.45 – 14.00 Analysis of Antioxidant and Chlorophyll on Green Algae From Totok Bay
and Tongkaina Waters, North Sulawesi
Desy M.H. Mantiri, Rene C. Kepel , Antonius P. Rumengan

14.00 – 14.15 Metal Content and Pigments on Green Algae Ulva Prolifera O.F. Müller, in
North Sulawesi Waters
Desy M.H, Mantiri, Rene Ch. Kepel1

14.15 – 14.30 Padina autralis the edible brown seaweed from North Sulawesi coast as a
natural antioxidant source
Chatrien A. Sinjal

14.30 – 14.45 Genetic Population Structure of Jerbung Shrimp (Penaeus merguensis De

Man 1988) In Fisheries Management Area, Indonesia Country 573
Melfa Marini, Ali Suman, Achmad Farajallah, Yusli Wardiatno

14.45 – 14.00 The availability of plankton In manado bay

Joice R.T.S.L. Rimper, Silvana D. Harikedua

15.00 – 15.15 Coffee Break

10
PARALLEL SESSION D – Local Knowledge
Kolbano Room

12.30 – 12.45 The use of convently red laru mur as local yeast sources in bread making
without cassava flour substitution
Regina I.M. Banoet, I. Medah, Mesry Manafe, I Made Sudana, G. N. Alit
Susanta Wirya

12.45 – 13.00 Ethnobotany study of natural dyes by women in Hundihopo village, East
Rote District, Rote Ndao Regency
Daud. Y, Sabuna Ch. A, Ngginak. J

13.00 – 13.15 Morphometric analysis, density and diversity of macroalga in Dara Mara
marine waters, Anarae Vilage, Nuse Rote Ndao
Alfred G. O. Kase, Anggreini D.N. Rupidara, Sony R. Klila, Alan Ch.
Sabuna, Anthoinette R. F. Anakotta, Yohanes Meryanto, Imanuel J.
Emola

13.15 -13.30 The analysis of quantitative semi active components extract of gorgonian
sea plumes (Rumphella sp and Hicksonella sp) uses thin layer
chromatography-densitometry method
Yunialdi Hapynes Teffu, Ruddy Suwandi, Nurjanah

13.30 – 13.45 Utilization of local isolate entomopatogen fungus to control Cylas

formicarius fabricus in sweet potato
Nina Jeni Lapinangga, Chatlynbi T. Br. Pandjaitan, Jaqcualine A. Bunga,
Jimrift H. H. Sonbai, Yosefus F. Da Lopez

13.45 – 14.00 Integrating local music, craft and games into the mathematics curriculum
in timor-leste: how teachers and students are motivated
Domingas Ximenes Belo

14.00 – 14.15 Exploration study of the pigment types of crab stone Grapsus
albolineatus as pharmaceutical and cosmetics materials
Darus Saadah J. Paransa, Desy M H. Mantiri, James J.H. Paulus, Cysca
Lumenta, Medy Ompie, Suria Darwisito, Alfread Kase

11
 LIST OF POSTERS

No. Title
1 Extracellular Lipase of Schleichera oleosa Seedas Eco-friendly Biocatalist
Mellissa E.S.Ledo, Hartini, R.L. Sole, Merpiseldin Nitsae
2 Biodiversity of Soil Microfungi in Natural Park of Baumata
Ewinda I.Feni, Mellissa E.S.Ledo, Arnold Ch.Hendrik
3 Manggulu, a Mix Banana Peanuts and Palm Sugar Bar, a Local Food of East Sumba
Maria Pada, Adriana Tunliu and Mefibosed Radjah Pono
4 The Influence of Different Cropping Patterns and Irrigation Techniques on
Productivity of Tomato Plants (Lycopersicum esculentum, Mill)
Olivina Sofia Messakh and team
5 Development of Minapolitan Area Based on Tuna Fisheries in Flores Timur
Regnecy, East Nusa Tenggara Province
Wilson L.Tisera, Johanes W.Kiuk, Welma Pesulima, Ovie Ningsih, Mario R.Naguit
6 The Histological Change of Cunang (Muarenesox talabon) Swim Bladder after
Drying Preservations
Dewi S. Gadi, Adi WInanrto, Wini Trilaksani, Tati Nurhayati
7 Sea turtle Potency in East Nusa Tenggara (A Case Study : Rote Ndao Beach, Sabu
Rajua Beach, Oebelo Beach and Teres Beach, Kupang Regency)
Fani I.Ginzel, Wilson L.Tisera, Alfred G.O.Kase, Donnie M.Bessie
8 Profile of Macroalgae Availability in Rote Barat Waters, Rote Ndao Regency
Fani I.Ginzel, Wilson L.Tisera, Alfred G.O.Kase, Donnie M.Bessie, Rocky L.L.Supit

12
14.15 – 14.30 Protein, amino acids and organoleptic quality of sardine bakasang with
pineapple puree introduction
Ovie Ningsih, Umbu P.L. Dawa, Yulian Abdullah

14.30 – 14.45 The growth of Eucheuma spinosum in different depth of Batubao marine
waters West Kupang
Alfred G. O. Kase, Apriliana Ballo, Anggreini D.N. Rupidara, Jeni
Kambajawa, Anthoinette R. F. Anakotta, Yohanes Meryanto, Darusaadah
Paransa, Kurniati Kemer

13
Day 1:
Agenda Venue
Sept 3, 2018
4.00 – 8.00 Meeting and Dinner of Swiss
Consortium Members: Bellin
1. Rector of Instituto Catolico Hotel
Para a Formacao De
Professores, Timor Leste
2. Director of Citra Husada
Mandiri Kupang Medical
Science Institute , Kupang
3. Rector of Flores University,
Flores
4. Rector of Wira Wacana
Christian University, Sumba
5. Rector of Tribuana
University, Alor
6. Rector of Nusa Cendana
University, Kupang
7. Rector of Widya Mandira
Catholic University, Kupang
8. Rector of Mataram
University, NTB
9. Head of Marine and
Fishesries Department, NTT
Province

14
Day 2:
Agenda Venue
Sept 4, 2018
08.00 – 12.00 Dies Natalies of Artha W acana Cam pus
Christian University of UKAW
12.00 – 12.15 Signing M oU of Consortium : Cam pus
1. Rector of Instituto Catolico of UKAW
Para a Form acao D e
Professores, Tim or Leste

2. Director of Citra Husada

M andiri Kupang M edical
Science Institute , Kupang

3. Rector of Flores University,

Flores

4. Rector of W ira W acana

Christian University, Sum ba

5. Rector of Tribuana
University, Alor

6. Rector of Nusa C endana

University, Kupang

7. Rector of W idya M andira

Catholic University, Kupang

8. Rector of M ataram
University, N TB

Head of M arine and

Fishesries D epartm ent, N TT
ProvinceTribuana Universit
12.15-12.30 Closing of Dies Natalies of Cam pus
AW CU of AW CU

12.30-13.30 Lunch of Consortium Nelayan

Restaurant

15
 ABSTRACTS

EFFECT OF CLIMATE CHANGE, LAND USES AND

GEOLOGY ON SOIL ORGANIC MATTER AND CARBON
SEQUESTRATION IN DRYLAND, KUPANG REGENCY-
EAST NUSA TENGGARA
Melinda R.S. Moata, Aydamel Takalapeta, Yofris Puay, Charles
Tlonaen

Dryland Agriculture, Kupang state Agricultural Polytechnic

E-mail: melinda.moaat@staff.politanikoe.ac.id;
melinda.moata@gmail.com
.

Climate change had been affecting on land productivity especially in

dryland agriculture. Soil organic matter (SOM) content and its carbon
(C) sequestration are the indicators of land productivity. Stoichiometry
between C and soil nutrients (nitrogen and phosphorus)-C:N:P of SOM
plays important role for estimating nutrients mineralization and
immobilization processes in the soil. However, limited studies work on
the Stoichiometry C:N:P of SOM in dryland, whereas dryland farming
is crucial since covered almost all the land in East Nusa Tenggara.
Therefore, this study aims to evaluate the effect of climate change on
dryland productivity.
There were some variables such as rainfall (R), temperature (T), C, N,
P, and physical soil properties. This research was conducted at Kupang
regency areas on different land uses, parent material and slopping.
Overlay of several maps from Geographic Information System (GIS)
were applied to determine 15 sampling site that consisted of 11 parent
materials (geology), 3 land uses and 5 slopping types. Soil samples
were collected at 0-5 and 5-10 cm of top soil and analyzed at
laboratory.
The results showed that linear correlation between climatic factors (R,
T), parent material and slopping types on C, N, P in dryland soils.
Then, those factors had been affected to the Stoichiometry C:N:P of
dryland SOM. This ratio is crucial for determining mineralization and
immobilization processes the C and nutrients storage in the soils. This
information will be benefits for farmers or agricultural practices and
16
government on estimating level of soil fertility and health and fertilizer
needs.

Keywords: soil organic matter, climate, geology, dryland

17
 CLIMATE CHANGE ADAPTATION STRATEGIES
THROUGH DEVELOPMENT OF ALTERNATIVE
LIVELIHOODS IN ORDER TO IMPROVE TRADITIONAL
FISHERMEN HOUSEHOLD INCOME IN THE BORDER
AREA OF BELU REGENCY
Chaterina A. Paulus1*, M.R Pellokila2, Y.U.L Sobang3, E.
Azmanajaya4
1
Department of Aquatic Resource Management, Faculty of Marine
Science and Fisheries, Nusa Cendana University, Indonesia
2
Faculty of Agriculture, Nusa Cendana University, Indonesia
3
Faculty of Animal Husbandary, Nusa Cendana University, Indonesia
4
Polytechnic State of Balikpapan, Indonesia

*Correspondence E-mail: chatepaulus@staf.undana.ac.id

The impact of climate change is causing obstacles for fishermen

because of the increasing risk of fishing, changing seasons and fishing
locations, uncertainty of the wind season, thus affecting the number of
catches. Climate change conditions that disrupt marine ecosystems
certainly can worsen the economic life of fishermen households. The
decrease in fish catch will have implications on household income.
Fishermen in Belu have dependence on fishery sector especially
capture fishery. How fisherman adaptation to climate change requires
a strategy in encouraging alternative livelihoods. One of the economic
adaptation strategy that fishermen can do is adaptation of human
resources in fishery households through economic activities outside
the fishery.
This research aims to determine the income of fishermen households,
analyzed the contribution of alternative efforts to total household
income and analyze the involvement of family members of
fishermenand the time spent to perform alternative livelihoods in the
border areas of Indonesia and Timor Leste. The research was
conducted using survey method through interview and observation
technique.
The results suggest that alternative livelihoods should be developed as
a form of adaptation fishing households in retained revenue caused by
18
decreased income from the main livelihoods as fishermen due to
climate change.

Keywords: adaptation strategies, climate change, alternative

livelihoods, fishermen income

19
 CLIMATE CHANGE ADAPTATION IN WATER SUPPLY
SECTOR THROUGH INNOVATIONTECHNOLOGY OF
WATER TREATMENT PLANT IN INDONESIA:
NUMERICAL AND EXPERIMENTAL INVESTIGATION OF
FILTER MEDIA IN DIRECT FILTRATION
Emil Azmanajaya1*, Chaterina A. Paulus2, Maria R. Naguit3,
Wilson L. Tisera4
1
Polytechnic State of Balikpapan, Indonesia
2
Nusa Cendana University, Indonesia
3
Jose Rizal Memorial State University, Phillipines
4
Artha Wacana Christian University, Indonesia
Correspondence author: emil.azmanajaya@poltekba.ac.id
Climate change has a difficult impact to avoid in many aspects of life.
Extreme impacts of climate change are the rise in temperature as well
as shifting seasons. Climate change causes an imbalance of clean water
availability. Climate change causing floods during the rainy season and
droughts in summer. Flooding causes raw water becomes very dirty
and difficult to be processed into clean water. Drought causes high
groundwater use. The use of groundwater that has a high metal element
is very dangerous to human health. In order to maintain the long-term
availability of clean water for the community, it takes technology
innovation in water treatment systems.
This research was conducted to investigate the filter media at the water
treatment plant through physical laboratory testing and the
implementation of the numerical model of sediment transport in porous
media. The physical test model named “Pilot Plant Direct Filtration”
and the numerical modelling utilizes the equation of Darcy velocities
and sediment transport equation of particles flowing through porous
media for 1 dimension.
The results of this research are expected to be useful for the provision
of clean water in facing the clean water crisis due to climate change in
Indonesia.

Keywords: climate change, water treatment plant, direct filtration,

provision clean water

20
 ECOLOGY STATUS OF MANGROVE IN ROTE-NDAO
REGENCY
Donny M. Bessie1, Wilson L. Tisera1, Alfred G. O. Kase1, and
Fanny I. Ginzel1
1
Faculty of Fisheries and Marine Sciences, Artha Wacana Christian
University

Corresponding author: donny_ikan@yahoo.co.id

Mangrove forests are commonly grown in brackish aquatic swamps

along shorelines and affected by tidal seawater. Mangroves grow in
places where there is puddling and organic material accumulation. In
general, mangroves well-develop in areas that are protected from the
onslaught of the waves, or in the mouth of the river where the water
flow slows down and deposits the mud they carry from the upstream.
The mangrove community has a contribution in supporting fisheries
production, but the increasing human need to transform mangrove
ecosystem into residential, industrial, and recreation areas has caused
economic and ecological conflict of interest. Therefore, this study aims
to analyse community structure of mangrove to become a source of
information and data in supporting the management of fisheries
resources in Rote Ndao Regency.
The method used was survey method which is direct observation of
mangrove vegetation and community activity in the utilization of
mangrove.
This study found 8 species of mangroves, where the mangrove density
was rarely densely packed with density ranged between 80-2,500
trees/hectare. The types of Sonneratia alba and Rhyzophora apiculata
were the types that have great influence on the mangroves community
in Rote Ndao District.

Keywords : Mangroves, Community Structure, Density, Rote-Ndao

21
 ANALYSIS OF HARMFUL ALGAL BLOOMS (HAB'S)
PHYTOPLANKTON CONCENTRATION IN COASTAL
AREAS OF KUPANG BAY
EAST NUSA TENGGARA
Welem Yupiter Giri1, Yohanes Merryanto S.1, Rockie R. L.
Supit1

Water Resources Management Study Program

1
Faculty of Fisheries and Marine Sciences, Artha Wacana Christian
University Kupang
Adisucipto Oesapa Street, PO. Box 147, Kupang-NTT, Phone/Fax
0380-881360
E-mail: welgyesoepeem@gmail.com

This study aims to analyze the phytoplankton concentration of

potential species that give rise to HAB's in supporting the management
of the potential of fishery resources on the coast of Kupang Bay. This
research has been carried out in the waters of Kelapa Lima, the estuary
of the Oesapariver (PohonDuri) and the estuary of the Tarus river
(Manikin beach) from May to June 2018. The collection of water
samples in the waters from the coastline taken using a modified Nansen
bottle with a volume of 10 liters and repeating 50 liters vertically
representing the surface, mid and bottom of the waters. The samples
obtained were filtered using 20 μm of plankton-net, with a
concentration formalin of 4%. The composition of HAB's species of
phytoplankton found in Kupang Bay waters consisted of 19 species
namely PSP 1 species, DSP 2 species, NSP 1 species, ASP 1 species,
CFP 4 species, Red Tide 9 species and Anoxia and Hypoxia 7 species.
The total abundance of overall phytoplankton HAB's potential species
in the waters of TelukKupang ranged between 1052.8-5760 cells/L
divided into groups of toxin-producing phytoplankton 11 species
(1017.6-5734.4 cells/L) and phytoplankton groups causing Red Tide 9
species (25,6-1033,6 cells/L). HAB's species phytoplankton species
diversity index (H') in these three locations is high, evenly distributed
species (E) and no species dominates (C). The predominantly potential
phytoplankton species of HAB consists of 4 species,
Trichodesmiumerythraeum and Trichodesmiumthiebautii from the
Cyanobacteria class, whereas from the class Bacillariophyceae

22
(Diatom) consists of Skeletonema spp. and Chaetoceros spp. In the
Tarus waters (Manikin Beach) have experienced the bloom of
Trichodesmiumerythraeum and in the waters of Oesapa (PohonDuri)
have experienced the bloom of Trichodesmiumthiebautii.

Keywords : phytoplankton, toxin-producing, causes of Red Tide,

potential species of HAB's, Kupang Bay.

23
 PROSPECT FOR TRANSBOUNDARY SMALL PELAGIC
FISHERIES MANAGEMENT OF INDONESIA-TIMOR LESTE
IN OMBAI STRAIT
Beatrix M. Rehatta1,3, M.M. Kamal2, M. Boer2, A. Fahrudin2 dan
Zairion2

1
Department of Coastal and Marine Resource Management,
Graduate School, Bogor Agricultural University, Bogor
INDONESIA
2
Faculty of Fisheries and Marine Science, Bogor
Agricultural University, Bogor INDONESIA
3
Faculty of Fisheries and Marine Science, ArthaWacana
Christian University, Kupang, NTT INDONESIA
Email: beatrixrehatta@gmail.com

The small pelagic fisheries of the Ombai strait offer a pivotal role for
fishers and local communities in the Indonesia-Timor Leste, but
uncontrolled fishing practices may cause copious number of
problems.Regardless proper management, such condition enables to
generate further problems associated with alleviation of fish resource
and ecosystem services, which in turn capable of causing disturbances
in several aspects such as economic, social, politic, and security. In this
regard, there is a clear necessity to establish a collaborative
management between Indonesia and Timor Leste in order to manage
small pelagic resources. Several important factors that could
strengthen the cooperation between two countries included
transboundary resources, identic fishery characteristic (dominated by
small-scale fisheries) and location, presence of informal agreement and
co-operation among traditional fishers in fishing activities, and strong
kinship, relationship between communities in boundary areas of
Indonesia and Timor Leste. Therefore, in order to support
transboundary small pelagic fisheries management, collaboration
related to comprehensive studies and development of management
regime is required.

Keywords: Ombai strait, small pelagic, transboundary fishery

management

24
 MANGROVE FOREST HABITAT CONDITIONS IN TANAH
MERAH COASTAL BEACH EAST NUSA TENGGARA
Jeriels Matatula1, Meilyn Renny Pathibang1, Ni Kade Ayu Dewi
Aryani1, and Alfred Umbu Kuala Ngaji2*
1
Department of Forestry, State Agricultural Polytechnic of Kupang,
Jl. Prof. Herman Yohanes-LasianaKupang
2*
Department of Dry Land Farming Management, State Agricultural
Polytechnic of Kupang, Jl. Prof. Herman
Yohanes-lasianaKupang. (Speaker)
Email : Jerielsforestry@gmail.com

Mangrove ecosystem is a group of plant species that grow along the

tropical coastline until subtropical and also a natural resource that must
be maintained, so that it can support the development and can be
utilized optimally with sustainable management.
This research was conducted in mangrove forest on the coast of Tanah
Merah, which aims to determine the condition of mangrove forest
habitat. Data were analyzed by multiple linear regression to determine
the effect of habitat quality on high growth of mangrove plant. The
research location is 30 ha with horizontal length of 1500 meters and
vertical 200 meters. Determination of plot sampling were
systematically. The measuring plot is made from the ground to the sea
vertically with a size of 5m x 5m. Number of plots were 45 plots.
The results showed that the type of mangrove forest in Tanah Merah
coastal area is Sonneratia casoelaris, Avecenia alba, Avacenia marina
and Avecennia lanata. Environmental factors that influence the high
growth of mangrove in the research location include mud thickness,
texture, temperature, total N, P, K, organic matter, water pH, Slope,
Length of tide, Salinity and DO. Habitat conditions greatly affect the
growth of mangroves on the coast of Tanah Merah.

Keywords: Habitat, Mangrove, Coastal

25
 THE GROWTH OF RED ALGAE Kappaphycusalvarezii
Doty (Doty) WITH LONG LINE METHOD IN DIFFERENT
DEPTH IN BATUBAO MARINE WATERS,TESABELA
VILLAGE, WEST KUPANG SUBDISTRICT KUPANG
REGENCY
Alfred G. O. Kase1,* Daud Yanti2, Kalorang. M2. John Siregar1,
Lebriana Boikh1. Dessy Mantiri3
1
Aquatic Resources Management, Department of
FisheriesandMarineScience, ArthaWacana Christian
University.Adisoecipto Street, Oesapa, Kupang - Indonesia.
2
Biology Department of, ArthaWacana Christian
University.Adisoecipto Street, Oesapa, Kupang - Indonesia
Corresponding email: kasealfred@yahoo.com

This research is to cultivate the red algae Kappaphycus alvarezii based

on different depth. The research aims to know the growth of alga and
several environmental factors that affect algal growth. The observation
and experimentalmethod were used on this research. Long line
cultivation method, binding 60 seeds of red at the depth of 10cm, 1m,
and 2m. The measurements were carried out every week for two
months. The Environmental parameters measured were temperature,
salinity, current velocity, pH, Nitrate and Phosphate.The results
showed that the average weight of algae occurred in the seventh week
at a depth of 10cm of 541gr, at a depth of 1m were 427gr and at the
depth of 2m were 320.5 gr. The average daily growth of algae at a
depth of 10 cm is 9 gr, the depth of 1 m reaches 6.673 gr, while at depth
2m reaches 4.5 gr. The highest percentage of daily growth was
observed in the first week at 10 cm depth of 14.28%, and tended to
decrease at the last week of cultivation up to 2.04%.The decrease of
algae growth at the depth of 1m and 2m is thought to be caused by the
lack of light intensity and the attack of baronang fish which was the
herbivorous fish and known as main causes of growth decline of alga,
while the quality of the environmental factors was on the normal range
for algal growth.

Keywords : Kappaphycus alvarezii, growth, depth.

26
Escherichia coli QUALITY ON Tilapia Fish (Oreocromisniloticus)
CULTURED IN LAKE AND RIVER TONDANO
O. Ningsih1,F.G. Ijong2, S. Berhimpon2
1
Lecturer at Fisheries and Marine Science Faculty of Artha
Wacana Christian University, Kupang, East Nusa Tenggara
Province, ovieningsih@gmail.com
2
Lecturer at Postgraduate Department of Sam Ratulangi
University, Manado, North Sulawesi Province
Water pollution with presence of pathogen bacteria like Escherichia
colicould be done at Lake Tondano and River Tondano as consequence
of human activity. That condition made contamination to the tilapia
fish cultured at Eris Village, Regency of Minahasa and Ternate Baru
Village, Manado City. This study aims to assess the presence and
amount of the bacteria Escherichia colion tilapia fish that cultured
along the sites of Lake and River Tondano. Moreover, to compare the
quality of tilapia fish cultured both in Lake and River Tondano based
on the presence of the bacteria Escherichia coli. From the results ofthis
study concluded that in fish taken from farms in Eris Village, Minahasa
Regency and Ternate Baru Village, Manado City showed the value of
total bacteria(ALT) is still below the quality standard, butthe
totalbacteria of Escherichia col imore higher than the standard.
According to ALT value and total Escherichia coli showed that tilapia
fish cultured from Lake Tondano have a better quality than the tilapia
fish cultured from Tondano River.
Keywords: Lake and River Tondano, tilapia fish, E. coli,quality
standard

27
 THE ANTITUMOR ACTIVITY ONHALIMEDA OPUNTIA,
Halymenia durvillaei, Padina australis and Laurencia pappilosa
MARINE ALGAE FROM NAIN ISLAND OCEAN NORTH
MINAHASA REGENCYNORTH SULAWESI.

Kurniati Kemer, Esther D. Angkouw,

Khristin I.F. Kondoy, Desy M.H. Mantiri,

The waters of Nain Island in North Sulawesi has abundant aquatic

resources including marine algae. The presence of these resources in
the intertidal zone is highly important, because of their chemicals
content are beneficially as raw materials in food industry, cosmetics,
pharmacy and etc.
Various researchwas done in order to optimally sustainable the use of
these biological richness. Therefore, this research was conduct to find
out antitumor activity test on some algae species found in Nain island
waters. Types of algae that are samples of the research to be tested are
Halimedaopuntia, Halymeniadurvillaei, Padinaaustralis and
Laurencia papillosa.Antitumor activity test was performed by MTT
method [3, (4,5-dimethylthiazol-2yl) -2,5-diphenyltetrazolium
bromide]. The tumor cells used were HeLaphasage 30 tumor cells.
The results showed that Halimeda opuntia of methanol extract had
cytotoxic activity and was categorized as an active antitumor.

28
 THE SUPPLEMENTATION EFFECT OF PROBIO FMplus AS
SYMBIOTIC IN LIQUID AND SOLID FORMS ON THE
BROILER CARCASS QUALITY
Helda A. Y. Ninu, and Catootjie L. Nalle*
Animal Husbandry Department, Polytechnic of Agriculture Kupang,
Prof. Herman Yohannes St., Lasiana - 85228, Kupang, East Nusa
Tenggara, Indonesia

Corresponding author: catootjienalle@gmail.com.

Probio FMplus is a probiotic product made of a number of Lactobacillus

sp. bacteria multiplied in mix sago (putak) and lontar liquid sugar (nira
lontar) as prebiotics. This product which was made at Polytechnic of
Agriculture Kupang and Jambi University has not been intensively
evaluated for its effectivity. Thus, the main objective of the present
study was to evaluate the effect of probiotic FMplus in liquid and solid
forms on the quality broiler carcass.
In this study, total of 160 unsex broiler chicks housed in floor pens
from day 1 to 21 post-hatch. The experiment was designed by using
completely randomized design with four treatments and four
replications. The treatments were R0 (control), R1 (5 mL/L drinking
water), R2 (5 g/kg commercial diets), and R3 (5 mL/L drinking water
and 5 g/kg commercial diets). The commercial diets were offered ad
libitum in crumbles form to four replicate cages of broilers (10
birds/pen) from day 1 to d 21 post-hatching.
The results of the study showed that significant differences (P<0.05)
were observed in carcass yield, carcass percentage, water holding
capacity, the brightness level of carcass, and abdominal fat percentage.
No significant differences (P>0.05) were found in pH and cooking
shed. Birds given control treatment (no Probio FMplus) had lower
(P<0.05) carcass yield and carcass percentage compared to those given
probio FMplus through drinking water (R1, 5 mL/L), commercial diet
(R2, 5 g/kg), but it had similar (P>0.05) carcass yield with the
combination treatment (R3, 5 mL/L and 5 g/kg). Water holding
capacity of birds given Probio FMplus 5mL/L (R1) was significantly
(P<0.05) higher than three other treatments. The brightness level of

29
carcass of birds given control treatment (R0) was significantly
(P<0.05) lower than those given Probio FMplus. The abdominal fat of
birds given Probio FMplus (R1, R2, R3) was similar (P>0.05) but it was
significantly (P<0.05) higher than control treatment (R0). In
conclusion, with the exception of pH and cooking shed, the utilization
of probio FMplus in liquid or solid form or in combination produced
better carcass quality.

Keywords: probio FMplus, broilers, carcass quality

30
 APPLICATION OF THE MARINE FISH SCALE DERIVED
NANOCHITOSAN FOR INCREASING THE FOOD
SECURITY OF FISHERY PRODUCTS

Rumengan Inneke F.M. *1; Pipih Suptijah*2, Stenly Wullur*1,

Aldian Hein Luntungan*3, Fallen Billy Sandana *3 and Netty
Salindeho*1

*1Faculty of Fisheries and Marine Science, Sam Ratulangi University

*2Faculty of Fisheries and Marine Science, Bogor Agriculture
Institute
*3Postgraduate School, Sam Ratulangi University
Corresponding author: innekerumengan@unsrat.ac.id

Marine fish scales are abundantly available as wastes of many Sea

Food Restaurants along the coast of Manado Bay, North Sulawesi.
This study has been conducted to prepare nanochitosan and chitosan
from chitin extracted from the scales of some marine fish, and to apply
it for increasing the food security of fishery products such as raw and
smoked fish by evaluating its nutritional values and food hygiene. The
preparation of chitosan was following the standard steps of
deproteinization, demineralization and deacetylation of the chitin
extracted from fish scales. Gelatin ionic method was apply to prepare
nanochitosan by adding drop wise a tripolyphosphate-pentasodium
solution to chitosan solutions under stirring.
Chitin yields (%) of five marine fish species ranged from 17 to 61%,
and chitosan was obtained when the percentage of diacetyl degree
exceeds 50%. Chitosan and nanochitosan were molecularly proven
using Fourier Transform Infrared Spectroscopy (FTIR) within the
wave length of molecular standard. Diameter of nanoparticles was in
the range of 59 to 438 nm. Application of nanochitosan for evaluating
food security was performed by spraying onto the surface of the
products or immersing the product in nanochitosan solution. The
products coated with nanochitosan were found to have less total plate
count of bacteria and longer shelf life within the standard range of
nutritional values.

Keywords: marine fish, scales, chitin, chitosan, nanochitosan, food

security

31
 SUBJECTIVE QUALITY OF MACKEREL AND SNAPPER
FISHBALL CATCHED
IN LARANTUKA DISTRICT EAST FLORES
Yosephina M. J. Batafor1 and Gregorius Gehi Batafor2
1
Fisheries Technology Study Program, Larantuka Institute of
Teacher Training and Technology. Author corresponding:
yosephinabatafor@yahoo.com / 082145145952.
2
Agribusiness Management Program, Kupang State Agricultural
Polytechnic.
Author corresponding: gregorius.batafor@gmail.com

This study aims to obtain an objective presentation, especially about

the quality of mackerel and snapper fishball catcheds in East Flores
regency. The research was conducted at the Laboratory of Fisheries
Technology Study Program, Faculty of Technology Institute of
Teacher Training and Technology Larantuka (IKTL). The research
material used is mackerel and snapper fish, tapioca flour, and spices.
The tools used are analytical scales, meat milling machine, flour
grinding machine, pot basin, and gas stove. The research method used
is experimental method, where data is collected through experiment
and direct observation to the observed object. Subjective of the
observation by panelists of were based on appearance, elasticity, and
taste using the preference and acceptance tests The method used in
this research is descriptive qualitative method.
The results of the panelist's assessment using the preference test
scoring method indicated that the mackerel fish balls were selected
compare to snapper meat with criteria the value of the appearance of
7.40, the elasticity value of 7.33, and the taste value of 7.26, so it is
suggested that there is an appropriate formula and prescription formula
for the manufacture fishball will produce excellent fishball and it is
expected that there will be further research on the making of fishball
using other kind meat fish catches in East Flores Regency and adjusted
to SNI 01-3819-1995.

Keywords: fish ball, fish meat, appearance, elasticity, and taste

32
 Security Assessment and Acceptance Levels on Se'i Tuna
Products Processed with Different Material and Concentration of
Liquid Smoke
Ayub U.I. Meko1, Melkianus Nuhamara2, Mada M. Lakapu1
1
Faculty of Fisheries and Marine Sciences, Artha Wacana Christian
University Kupang
2
Faculty Technology of Agriculture, Artha Wacana Christian
University Kupang

Corresponding author: ayubmeko@yahoo.co.id

Meat se'i products in Kupang City are generally processed using

special wood smokers, namely Kesambi (Schleichera oleosa). The
wood parts used are stems and leaves. The products appearance will be
very interesting and preferred when smoked with wood and leaves of
Kesambi.
The continuously use of this wood will threaten its population.
Modification of smoked meat continues to be developed using liquid
smoke to obtain safe and uniform products in each production period.
A study was carried out to obtain liquid smoke from Kesambi stems
and leaves and then applied to the manufacture of tuna se'i. The aims
of this study was to determine safety level of the liquid smoke and to
characterize tuna se'i which were processed with liquid smoke from
Kesambi stems and leaves at different concentrations.
The result showed that the average pH of liquid smoke from the leaves
5.6 was higher than 2.7 average pH the stems. PAH compounds such
as benzo(a)pyrene and benzo(a)antracene produced in the smoke was
0.01 – 0.14 ppb, while in the tuna se’i was not detected. The liquid
smoke is safely used in tuna se’i making because the PAH level is
below the required maximum limit of 5 ppb. Tuna se'i which is
processed with liquid smoke from kesambi leaves at a concentration of
0.9% v/v has a score of 8.2 or is very preferred compared to the smoke
from Kesambi wood.

Keywords: tuna Se’i, liquid smoke, kesambi leaves, food safety

33
FOOD SECURITY ANALYSIS BASED ON LOCAL WISDOM
ON INLAND BOTI TRIBE IN DISTRICT KIE, SOUTH
CENTRAL TIMOR REGENCY
Jollyanes Petrecia Ledo1, Lebrina Ivantry Boikh*2,Sri Fatmah
Sari3
1
Programme of Theological EducationScience, University of Artha
Wacana Christian. Oesapa-Kupang, NTT, Indonesia
2
Programme of Aquatic Resources Management,Faculty of Fisheries
and Marine Sciences, University of Artha Wacana Christian. Oesapa-
Kupang, NTT, Indonesia.
3
Department of Fisheries Product Technology, Faculty of Fisheries
and Marine Sciences, University of Muhammadiyah Kendari,
Kendari.
Corresponding author: jollyledo@yahoo.com,
rini.boikh88@gmail.com
Boti tribe is a descendent from the indigenous tribe of Timor Island
whose life is still depend on nature and strong customs. Livelihood of
Boti tribe community is farming. The objectives of this study were to
identify food security conditions, analyzee the adequacy of food
availability and determine variables as indicators that can lead to
problems of food security.
A descriptive method was used as the research method. While the
sampling technique was accidental sampling technique. Samples
collected were 30 people of original Boti Tribe. Data used were
primary and secondary data. Data collection techniques were:
observation, structured interview and documentation.
By percentage analysis, the result of this research showed that food
security level of Boti tribe; 37% is in susceptible category, 30% food
resistant, 23% less food and 10% food insecurity. There was a
correlation between the level of household food security with age,
education level, number of household members, household income and
local wisdom of Boti tribe.

Keywords : food security, local wisdom, Boti tribe

34
 GROWTH ANALYSIS OF RED ALGAE Eucheuma
denticulatum (BURMAN) COLLINS & HERVEY, FARMED
USING DIFFERENT METHOD AND DIFFERENT STRAINS
IN NITBAUN VILLAGE, SEMAU ISLAND
Wilson L. Tisera1, Chaterina Paulus2, Ambariyanto3
1
Universitas Kristen Artha Wacana
2
Universitas Nusa Cendana
3
Universitas Diponegoro

Corresponding author: wilson_tisera@yahoo.com

Seaweed mariculture of Eucheuma denticulatum (trade name:

Eucheuma spinosum) is on of the main commodity from Semau
Subdistrict. , East Nusa Tenggara Province.
The objective of the study was to analyse and to compare the growth
of E. denticulatum farmed using longline and off buttom methods.
This study was conducted in one of cultivation areas in Semau
Subdistrict, East Nusa Tenggara Province.
The results showed that the growth rates of E. denticulatum farmed
using longline method was slightly faster and appeared to be better in
thallus morphology compared to off buttom method. Specific Growth
Rate (SGR) of E. denticulatum farmed using longline was 4.13% ± 1.5
day-1, while off buttom showed 3,70% ± 2.4 day-1. In terms of different
strains, between brown and green strains. E. denticulatum brown strain
showed better growth rate compared to the green one. Physical and
chemical parameters of the waters, included temperature, salinity,
transparancy and currents showed in optimal ranged.

Keywords: Growth rate, Eucheuma denticulatum, Longline, Off

buttom, Nitbaun village

35
 PRODUCTION OF RENEWABLE FUEL THROUGH
DECARBOXYLATION OF Pongamia pinnata L. SEED OILS.

Neonufa G. F*,1), Prakoso T2) and Neonufa N. E. M3)

1)
Department of Agriculture Product Technology, Universitas Kristen
ArthaWacana, Kupang, Nusa Tenggara Timur, Indonesia
2)
Department of Chemical Engineering, Institut Teknologi Bandung,
Bandung Jawa Barat, Indonesia
3)
Department of Dry Land Agricultural, Politeknik Pertanian Negeri
Kupang, Nusa Tenggara Timur, Indonesia

Corresponding author: godliefneonufa@gmail.com

The diminishing reserves of fossil fuel, indecision in its availability

and global warming issues has interested many researchers to look for
the renewable source of fuel. The main source of renewable liquid fuel
was oils of the seed crops. One of the oilseed crops that hasd been
studied is Pongamia pinnata L. Oil content and fatty acid composition
are important attribute desirable in seed oil. Monounsaturated fatty
acids are the best components for biodiesel (i.e. oxygenate fuel) when
considering the low temperature fluidity and oxidative stability.
However, biodiesel derived from vegetable oils always consists of
significant amounts of polyunsaturated and saturated fatty acids alkyl
esters, which hampers its practical applications.
Therefore, in order to increase added value of vegetable oils, the
oxygenate fuels (e.g. biodiesel) should be modified to bio-hydrocarbon
fuel (e.g. green diesel) which has similar properties to diesel fuel from
fossil.In addition, straight-chain saturated hydrocarbon molecules (n-
paraffins) should be as well as modified to be branched hydrocarbons
to lowering the freezing point and low temperature fluidity of the
renewable fuel.Therefore, the renewable fuel applications become
more widely. The objective of this work to evaluate the bio-
hydrocarbon (renewable fuel) profile that resulting of decarboxylation
of Mg-Zn basic soap based on Pongamia pinnata L seed oil originate
of Kupang.

36
It was found that oil content in Pongamia pinnata L. seeds originate of
Kupang was 36.01 % by weight. The monounsaturated compounds
contained in the oil approximately 30 %. Decarboxylation of Mg-Zn
basic soap resulting high bio-hydrocarbon with main carbon chain
length are were 15 (15C).

Keywords: renewable fuel, Pongamia pinnata, decarboxylation, seed

oil

37
 FOOD WATER TOTAL FACTOR PRODUCTIVITY
GROWTH TOWARDS FOOD SECURITY IN WEST TIMOR
Jonathan E. Koehuan1), Bambang Suharto2), Gunomo
Djoyowasito 3), and Liliya D. Susanawati4)
1
Department of Agricultural Engineering, Artha Wacana
Christian University Kupang – Indonesia
2
Department of Agricultural Engineering, Brawijaya University
Malang- Indonesia, Department of 3Agricultural Engineering,
Brawijaya University Malang- Indonesia,
4
Department of Agricultural Engineering, Brawijaya University
Malang- Indonesia

Corresponding author: jekoehuan@gmail.com*

It is believe that one of the prominent ways to boost food security in

semi arid region like West Timor where water is scarce is by the
increasing of food water productivity or to increase food production
with less water. This study makes a remarkable contribution with
taking into consideration food water TFP growth with its components
of efficiency change and technology change.
This study furthermore focuses on the estimation of food water
productivity and the estimation of food water TFP growth. To doing
so, 16 years secondary balanced panel data of climate and non climate
data were used. We applied FAO Penman-Monteith method in
estimating crop water use as the denominator of water productivity
subsequently stochastic frontier analysis- Malmquist index in
estimating food water TFP growth and its decomposition.
The results show that during 2000-2015, food water productivity in
terms of production per crop water use showing a fluctuated trend, with
averaging WPPaddy, WPCorn and WPFood which is aggregated of paddy
and corn are 0.459 Kg rice/m3, 0.792 Kg kernel/m3 and 0.458 Kg
rice/m3 respectively. Averaging TFP growth for WPPaddy, WPCorn and
WPFood are 1.003, 0.996 and 1.015 respectively. It is important to note
that the TFP growths are mostly influence by technology growth rather
than efficiency growth. The municipal of Kupang has the higher TFP
growth compared to other districts. Therefore the improvement in
production technology, better access to production input, and

38
enhancing farms management are the key to sustain food security in
West Timor.

Keywords: stochastic frontier analysis, technology change, WPCorn,

WPFood, WPPaddy

39
 FUZZY SET APPLICATION OF ENVIRONMENTAL
SUITABILITYINDEX FOR RICE PLANT (ORYZA SATIVA L)
BASED A GEOGRAPHIC INFORMATION SYSTEM TO
SUPPORT SUSTAINABLE LAND USE PLANNING
Tiwuk Widiastuti1 and Rony Stenly Mauboy2
1
Faculty of Computer Science , Nusa Cendana University:
tritiwuk@gmail.com
2
Faculty of Biologi , Nusa Cendana University:
rony_mauboy@gmail.com

The increased use and capability of geocomputation tools as well as

the increasing availability of land resource information
offerstheopportunity to utilize suchthesetools for the effective
management for planning purposes. One is the development of
spatially-based models that can be used to efficiently assess land
suitability based on available information. This paper presents a land
suitability evaluation study using the fuzzy set approach for rice field
(Oryza Sativa L.). The aim of this study was to evaluate the suitability
oflandfor rice cropsbased on the land characteristics.
The analysis was done using Geograhic Information System this
research was conducted at Kupang Regency, East Nusa Tenggara
Province.In this studycalculated land suitability index for temperature,
precipitation, soil texture, soil depth and slope.
The result showed that the study area of Kupang Regency had actual
physical land suitability classes for rice field in the S1( very suitable,
fuzzy set index > 0.4-0.6) class with an area of 17.363 km2, S2
(suitable, fuzzy set index > 0.6-0.8 ) class with an area of7.065 km2
and S3 (marginally suitable, fuzzy set index ) class of 0,2507km2.

Keywords: Land suitability, fuzzy set, rice field, Geographic

Information System

40
 BIOMASS ESTIMATES AND SPECIES DIVERSITY OF
MANGROVE FOREST IN TRANG, SOUTHERN THAILAND
Pablina Cadiza,b and Ponlachart Chotikarna,b,*
a
Marine and Coastal Resources Institute, Prince of Songkla
University, Songkhla, Thailand 90112
b
Coastal Oceanography and Climate Change Research Center,
Prince of Songkla University, Songkhla, Thailand 90112

*Corresponding author: ponlachart.c@psu.ac.th

Trang located in Southern part of Thailand, facing Andaman sea.

Mangrove conservation in this area is one of the prime initiatives being
4th on the list. Biomass and species diversity were investigated in 10-
25-YO and >50-YO mangrove forests in Sikao, Kantang and Palian
Districts. Based on the 30 plots (100 m2) sampled for biomass and
diversity, highest biomass was obtained in Rhizophora apiculata
(4,166.8372± 538.98 ton ha-1) and Avicennia lanata (3,292.72 ±
1,496.81ton ha-1) both in >50-YO mangrove forests in Kantang . The
>50-YO mangroves in Palian District also showed higher biomass of
the A. officinalis (3,855.46 +2,260.00 ton ha-1). The Species Diversity
Indexes (H’) in Palian, Kantang and Sikao were 1.82, 1.21 and 0.84,
respectively. The Rhizophoraapiculata in 10-25 YO in Kantang
obtained highest Dominance Index (C) of 1.00 and the lowest H’ value
of 0.00. The 10-25 YO mangrove forest had lower species richness
value of 4 compared to >50-YO with species richness value of 13.
Density of mature trees ranged from 560.71 ± 103.55 stems ha-1 in
Palian to 6220.00±459.78 stems ha-1 in Kantang. The basal area (BA)
in Kantang obtained the highest BA of 41.99 ± 37.03 m-2 ha-1. The
monospecific stands of R. apiculata in Kantang also had a BA of 35.58
± 3.91 m-2 ha-1.
Twenty-seven mangrove species and 15 mangrove associates species
were found. Of the 33-species recorded, two critically endangered
species, Bruguiera haenisii and Sonneratia griffithii and one
endangered species, Heritiera fomes were recorded in the surveyed
sites. The Ceriops decandra and Phoenix paludosa were recorded
asNear Threatened species.
Overall findings of this study suggests that biomass and diversity in
natural mangrove areas are higher than planted sites. This study

41
optimized to continue the efforts of management and conservation of
mangrove forests, particularly in Trang.

Keywords: biomass, Species diversity, mangroves, vegetation

characteristics

42
MODELING AND PREDICTION OF FISH CATCH LANDING
IN SONGKHLA LAGOON, THAILAND USING ARTIFICIAL
NEURAL NETWORK, MULTIPLE ADAPTIVE REGRESSION
SPLINE AND K-NEAREST NEIGHBORS MODELS
Ha Thi Thu Hue1, 2, 3 *, Siriporn Pradit1, 3, Apiradee Lim4,
Thongchai Nitiratsuwan5
1
Marine and Coastal Resources Institute, Prince of Songkla
University, (MACORIN), Hat Yai, Songkhla 90112, Thailand.
2
Central Institute for Natural Resources and Environmental Studies,
Vietnam National University.
3
Coastal Oceanography and Climate Change Research Center, Prince
of Songkla University.
4
Faculty of Science and Technology, Prince of Songkla University,
Pattani.
5
Rajamangala University of Technology Srivijaya, Thailand
Corresponding Author: hathithuhue2001@yahoo.com. Tel: +66-
0910495786 +84-0987261294

Artificial Neural Network (ANN) model was chosen from three high
appreciated models to predict the fish catch landing in Songkhla lagoon
based on environmental factors gathered with the water parameters.
Quarterly data of fish catch landing and environmental factor, water
parameters during the period 2009 -2017 were used for this study. The
performance of the models was validated by comparing the predicted
data sets with the measured data.
The results showed that, the proposed ANN model provided the best
result can be used effectively to predict the fish catch landing. The
determination coefficient (R2) was 99.99% for training data and
93.12% of the testing data in neural network model. Again, this study
also confirmed rainfall, turbidity and salinity were the most important
factors effect on fish catch through Multivariate Adaptive Regression
Splines (MARS) model.This research plays important role in choosing
the suitable model for forecasting fish catch in the fisheries, which is a
very necessary tool for fishery sustainable development management.

Keywords: Nonlinear Regression models, Fish catch landing,

Forecasting

43
 ECHIURAN DENSITY AND DISTRIBUTION IN RELATION
TO TIDAL FLOW AND SEDIMENT ORGANIC MATTER
CONTENT AND GRAIN SIZE COMPOSITION

Clarissa T. Reboton, Janet S. Estacion*

Institute of Environmental and Marine Sciences
Silliman University, Dumaguete City 6200, Philippines
Corresponding author, janetsestacion@su.edu.ph
The Echiuran population in the intertidal flat of Tulapos Marine
Sanctuary, Siquijor (9.2842° N, 123.6421° E) was investigated in
terms of density in relation to tides (ebb vs flood) and the substrate
(sediment organic matter content and size composition). Higher
density was observed just after ebb tide (316 individuals 100 m-2)
compared to that recorded prior to flood tide (97 individuals 100 m-2).
Spatial concentration was noted in plots that were in close proximity
to seagrass and mangrove vegetations, both with generally higher
percentages of organic matter content. In plots with high organic
matter but low densities, abundance appeared to be related to sediment
size. Lower densities are associated with moderate to high amounts of
fine (silt, clay) or larger sediment size. Fine sand appeared to be the
favored sediment type. Density was significantly affected when
organic matter and sediment parameters were combined (H; p < 0.05)
suggesting both factors influence echiuran density and distribution at
the study site.
Keywords: Echiura, Sediment, Organic Matter, Density, Distribution

44
 A CASE OF FATAL ATTRACTION: CORALLIVORY BY
THE VIOLET CORAL SHELL, Coralliophila violacea ON
BRANCHING Porites cylindrica

Clarissa T. Reboton

Institute of Environmental and Marine Sciences Silliman University,

Dumaguete City
Corresponding author: idayreboton@yahoo.com

This study examines drivers of predation by the corallivorous snail

Coralliophila violacea on the Indo-Pacific coral Porites cylindrica.
Ex situ experiments show that majority of subject snails responded
very strongly to the tests: healthy coral (H) vs. mechanically-damaged
(MD) coral treatment (X 2 = 15.622, df = 1, p = 0.000, Ø = 0.459) and
healthy coral fragment (H) vs. solitary snail (SN) treatment (X 2 =
24.500, df = 1, p = 0.000, Ø = 0.875). Responsive snails actively
selected their prey at night (H vs. MD: 96%; X 2 = 30.963, df = 3, p =
0.000, Cramer’s V = 0.535; H vs. SN: 67%; X 2 = 24.444, df = 3, p =
0.000, Cramer’s V = 0.638), confirming their nocturnal nature. Fastest
responses were during periods of highest water temperature.
Significant preference towards damaged fragments (70%) over healthy
ones (30%) (X 2 = 8.963, df = 1, p = 0.003, Ø = 0.407) was attributed
to large amounts of mucus released by damaged fragments. Between
healthy fragments and choice snails, all responsive subject snails
preferred healthy fragments indicating the absence of chemical
attraction between conspecifics. Snails that gained contact with the
fragments attached and remained on the abraded sites or on the cut
edges reported as sink sites of photosynthetically-produced nutrients.
Overall, results suggest important implications on the current health of
coral reefs. Climate change and other human-related stresses may put
corals at risk to predation that may lead to their extirpation and
extinction, especially for highly preferred coral species preys.

Keywords: Coralliophila, Mucus, Attractant, Predation, Porites

45
 IMPROVE THE CAPABILITY OF TESTING OF EXPORT
FISHERY PRODUCTS WITH SMART COMPUTER
SYSTEMS

Sebastianus A. S. Mola1
1
Computer Science Program, University of Nusa Cendana
Corresponding author: adimola@staf.undana.ac.id

Fishery products to be exported must pass the testing stage at the

Animal Quarantine Center to ensure no spread of disease to the export
destination area. The spread of the disease to the destination area can
cause an outbreak, destroying the environmental conservation and the
economy of the area. The long-term testing process has an effect on
increasing costs and increasing the risk of death for live fishery
products.
In this research, developed a case-based smart system to reduce the
testing process time. The intelligent system is constantly learning all
the time to improve the ability to identify disease, both in terms of
computing speed and in reducing human error. This study aims to
compare several methods of indexing that are traditionally based on
the types of fishery products, probabilistic indexing with Naïve Bayes,
and indexing based on clustering with K-Means. The calculation of
case similarities tested by case-based case using Tversky [1977] using
the k-NN principle in decision making.
Test results with traditional indexing resulted in an accuracy of 94.6%
was the highest of the other two methods. In test time size, probabilistic
indexing reduces the computation time by nearly 50%. The worst
computing time by using traditional indexing is directly proportional
to the increase in the number of cases on the case base.

Keywords: fishery products test, intelligent computer system,

probabilistic indexing, K-Means clustering

46
 ANALYSIS OF ANTIOXIDANT AND CHLOROPHYLL ON
GREEN ALGAE FROM TOTOK BAY AND TONGKAINA
WATERS, NORTH SULAWESI

Desy M.H. Mantiri, Rene C. Kepel, and Antonius P. Rumengan

Faculty of Fisheries and Marine Science, Sam Ratulangi University,
Jln. Kampus Unsrat
Bahu, Manado 95115, North Sulawesi, Indonesia
Corresponding author: dmh_mantiri@unsrat.ac.id

The purpose of this study is to determine the ability of antioxidant

activity and chlorophyll concentration in green algae Halimedaopuntia
(Linnaeus) J.V. Lamouroux and H. taenicolaW.R. Taylor from the
Totok Bay. Totok Bay located in Southeast Minahasa Regency is a
mine waste disposal area. These waters have high turbidity and water
brightness only 26 cm,also the sediment containing metals exceeds the
quality standard. As a control, green algae are taken of the same species
from Tongkeina Waters, Manado City, relatively clear waters.
Antioxidant analysis useding DPPH method and the test was done by
using microplat 96 wells. For chlorophyll analysis, used UV-Vis
Spectrophotometer and HPLC.
The results obtained, after analyzed by Spectrophotometer Multiskan-
Go, the algae from Teluk Totok have antioxidant activity. Algae from
Totok Bay H. opuntia and H. taenicola have IC50 antioxidant activity
with incubation time 30' respectively of 16,249.90 ppm and 666.91
ppm. For the same species of Tongkeinawaters have IC50 antioxidant
activity with 30' incubation time of 1,433.85 ppm and 629.00 ppm
respectively. The mean chlorophyll-a concentration for H. opuntia and
H. taenicola from Totok Bay was 3.48 μg / ml and 5.96 μg/ml
respectively, while the same species algae from Tongkeinawaters were
5.25 μg/ml and 5.44 μg/ml.These results indicate that green algae can
grow and survive in contaminated waters.

Keywords: Halimeda, Antioxidant, Chlorophyll, Totok Bay,

Tongkeina Waters

47
 METAL CONTENT AND PIGMENTS ON GREEN ALGAE
Ulva prolifera O.F. Müller, IN NORTH SULAWESI WATERS

Desy M.H, Mantiri1 and Rene Ch. Kepel1

Faculty of Fisheries and Marine Sciences, Sam Ratulangi University,

Jln.Kampus Unsrat Bahu, Manado 95115, North Sulawesi, Indonesia
Corresponding author: desy_mantiri@yahoo.com

The purposes of this research were to analyze the metal content and
concentration of photosynthetic pigments in green algae U.
proliferafrom Totok Bay, and Blongko waters algaewas used as
control. U. proliferais one of the algae found in Totok Bay waters
growththroughout the year. Metals content in U. prolifera thallus and
sediment were analysed using APHA (2012) and USEPA (2005)
methods. Pigment concentration was measured using
spectrophotometer and HPLC.
The concentration of metal in thallus of U. prolifera in Totok Bay
waters iswere contained cadmium (Cd) 0.40 ppm, chromium (Cr) 1.00
ppm, copper (Cu) 2.00 ppm, and mercury (Hg) 0.63 ppm. While the
concentration of metal in Ulva sp. in Blongko waters is were cadmium
(Cd) 0,04 ppm, chromium (Cr) 0,020 ppm, copper (Cu) <0,50 ppm,
and mercury (Hg) 0,006 ppm. The concentration of chlorophyll a and
b foundin Totok Bay waters is were 6.59 g / ml and 0.14 g / ml, and
5.17 g / ml and 2.35 g / ml found in Blongko waters respectively.

Keywords: Metals, Pigments, Ulva prolifera, Totok Bay, Blongko

Waters.

48
 GENETIC POPULATION STRUCTURE OF JERBUNG
SHRIMP (Penaeus merguensis de Man 1988) IN FISHERIES
MANAGEMENT AREA, INDONESIA COUNTRY 573

Melfa Marini1,2*,Ali Suman3, Achmad Farajallah4, Yusli

Wardiatno5
1
Research institute for Inland Fisheries and Extention, Ministry of
Marine Affairs and Fisheries, Jl. Gubernur H.A Bastari No 08,
Kel.Silaberanti, Kec. Seberang Ulu I, Palembang, Sumatera Selatan,
Indonesia
2
Inland Fisheries Resources Development and Management
Department, Jl. Gubernur H.A Bastari No 08, Kel.Silaberanti, Kec.
Seberang Ulu I, Palembang, Sumatera Selatan, Indonesia
3
Marine Fishery Research Institute, Ministry of Marine Affairs and
Fisheries, Jl. Muara Baru Ujung, Kompleks Pelabuhan Perikanan
Nizam Zachman, Penjaringan-Jakarta 14440, Indonesia;
4
Department of Biology, Faculty of Mathematic and Natural
Sciences, Bogor Agricultural University, Jalan Raya Darmaga,
Kampus IPB Darmaga, Bogor 16680, West Java, Indonesia;
5
Department of Aquatic Resources Management, Faculty of Fisheries
and Marine Sciences, Bogor Agricultural University, Jalan Raya
Darmaga, Kampus IPB, Bogor 16680, West Java, Indonesia.

Corresponding author: melfa_marini@yahoo.com

Jerbung shrimp (Penaeus merguensis) is a shrimp penaidae high

economic value. The fishing activity of jerbung shrimp in the fishery
management area 573 of Indonesia (FMA RI 573) is very high, so it
needs to be done for sustainable management. The purpose of this
study is to determine the genetic population structure of jerbung shrimp
in the fishery management area 573 of Indonesia.
The total samples used were 31 samples obtained from fish landing
sites in July 2016 to January 2017. The result of genetic analysis shows
similar condition with morphological population structure analysis
result with truss network approach which has been done to shrimp
jerbung in FMA RI 573. For the purpose of analysis, sekunsing the
results of research combined with data sekunsing from GenBank.
The results of haplotipe tissue analysis showed that there were three
groups of jerbung prawns. The first group comprises a population of
49
southern NTT, a second group of members from the Arabian Sea,
Persian Gulf and Egypt, and a third group of populations from southern
Java and southern NTT. The genetic data of this acquired jerbung
shrimp can be a strong foundation for sustainable resource
management. Therefore, we conclude that there are at least two
different jelung shrimp populations in FMA RI 573, one in the south
of NTT and one in the south of Java.

Keywords: Genetic Population Struktur, Penaeus merguensis,

jerbung shrimp, FMA RI 573

50
 THE AVAILABILITY OF PLANKTON IN MANADO BAY

Joice R.T.S.L. Rimper, and Silvana D. Harikedua

Faculty of Fisheries and Marine Sciences, Sam Ratulangi University,

Jl. KampusBahu Manado, 95115

This research aim was to determine the distribution of plankton in

Manado Bay based on the species composition and the relationship
with environmental parameterssuch as temperature and salinity.
Sampling was done twice in four stations onin April and June.
Sampling locations were at the mouth of the river (river estuarine),
onin the middle of the bay, mangrove areas and the coral areas.
The results showed that the zooplankton found are were mostly
copepods and for thephytoplankton arewere diatoms and
dinoflagellates. Diatoms in the bay were consist of Chaetoceros sp.,
Rhizosolenia sp., Skeletonema sp., Coscinodiscus sp., Thalassionema
sp., Nitzschia sp., Navicula sp., Bacteriastrumsp., and Bidulphia sp. As
for the dinoflagellates are were Ceratium sp., Dinophysis sp., and
Pyrocystis sp. The average abundance of plankton at Manado bay
waters iswashighestat station TM3 (middle area of the sea) and lowest
at TM4 (reef). Average abundance of plankton at all station are
were:12.017 sel/m3 at TM1 (river estuary); 15.264 sel/m3 at TM2
(mangrove); 16.204 sel/m3 at TM3 (middle area of the sea or bay?);
11.585 sel/m3 at TM4 (reef).

Keywords: Composition, plankton, Manado Bay

51
 MOLECULAR TEST AND THE DIRECT USE
OF MUR RED LARU AS YEAST IN BREAD MAKING
Regina I.M.BanoEt¹, I Made Sudana², G.N. Alit Susanta Wirya²,
A.I.Medah¹ and Mesry Manafe¹
1
ArthaWacana Christian University Kupang, Jl.Adisucipto 147
OesapaKupang
2
Udayana University, Jl,P.B. Sudirman Denpasar Bali
Corresponding author:
reginailse74@yahoo.com;imadesudana74@yahoo.com

This is a collaboration research funded by Indonesian Higher

Education (PEKERTI) for two years programs (2017–2018). The
research partner wasUdayana University, Denpasar as a co-researchers
with ArthaWacana Christian University, Kupang.
The aims of this study were first; to get a molecular identification of
Sacharomycescereviceaemur of red larucollected from production
houses in Kupang District and Municipal of Kupang. Second; to
carried out the direct use test of mur of red laru as yeast in bread
making. Molecular identification was done in several stage such as
follow: 1. Genomic DNA extraction using Quick-DNA ™ Fungal /
Bacterial Miniprep Kit (Zymo Research, D6005); 2. PCR
amplification using my Taq HS Red Mix (Bioline, BIO-25047); 3. The
PCR product purification using DNA Clean and Concentrator
(ZymoResearch, D4003); 4.A bidirectional Sequencing to run the tests.
The direct use of mur of red laru then being tested as a substitute for
yeast; The mur was then taken using a plastic spoon, put in a jar, leave
it for 1 hour until it settles, remove the liquid until the only one
remaining is the mur slurry, wash the myrrh sediment with cold
cooking water (ratio of 1: 2); the mur then rinsed 5 times until there is
no the sour flavour left in the yeast substitute.
The results showed that molecular identification of mur of red laru
from Sikumana production houses in KupangCity has a 99% - 100%
similarity to Sacharomycescereviceae based on 10 Hit BLAST results
against NCBI in the gene bank database. Then mur of the red larufrom
the Sikumana production house was directly used as yeast in bread
making. Characteristics of bread as follow: moisture content

52
 (25,757%), development volume (14,927%). The scale of
organoleptic test: color (3,711) with hedonic scale really like, flavor
(3,822) hedonic scale really like, taste (3,756) hedonic scale is very
like and texture (3,844) hedonic scales really like it.

Keywords: Mur, Red laru, Sacharomyces cereviceae, Yeast

53
 MORPHOMETRIC ANALYSIS, DENSITY AND DIVERSITY
OF MACROALGA IN DARA MARA MARINE WATERS,
ANARAE VILAGE, NUSE ROTE NDAO

Alfred G. O. Kase1,*), Anggreini D.N. Rupidara2, Sony R. Klila2,

Alan Ch. Sabuna2, Anthoinette R. F. Anakotta1, Yohanes
Meryanto1, Imanuel J. Emola1.
1
Aquatic Resources Management, of
FisheriesandMarineScienceDepartment of Artha Wacana
Christian University.Adisoecipto Street, Oesapa, Kupang -
Indonesia.
2 Biology Department of, Artha Wacana Christian
University,Oesapa, Kupang – Indonesia

Corresponding author: kasealfred@yahoo.com

This research was about morphometric analysis of macroalgae, density

and diversity, located in marine waters of Dara Mara, Anarae Village,
Ndao Nuse Sub District, Rote Ndao Regency.
This study aims to identify and measure the morphometric,density and
diversity of macroalgae. Transect line method using quadrant were
used in this study.
The results showed that there were 21 macroalgae species consisting
of 11 species of Cholorophyta, 3 species of Phaeophyta and 7 species
of Rhodophyta. The highest species and relative density of macroalgae
were found in Caulerpa sp species which were 6.8 individuals/m2 and
51.127%,while the lowest were Codium edule, Halimeda opuntia,
Monostroma nitidum, Dictyota cervicornis, and Padina japonica,
which were 0.033 individuals/m2 and 0.250%. The diversity index
were low with the value of H '0.76, while the morphometric analysis
of each species showed significant differences in each type of algae,
presumably due to differences of environmental factors.

Keywords: Morphometrics, Macroalgae, Density, Diversity.

54
ETHNOBOTANY STUDY OF NATURAL DYES BY WOMEN
IN HUNDIHOPO VILLAGE, EAST ROTE DISTRICT, ROTE
NDAO REGENCY
Daud. Y, Sabuna Ch. A,Ngginak. J
Biology Study Program, Teacher Training And Educational Faculty,
Artha Wacana Christian University. Jl. Adisucipto, Oesapa,
Kupang – NTT. PO BOX 147 Ponsel. +6282236331421
Correspondence author: James_ngginak@yahoo.com Tel.
+6285239196905

Ethnobotany Study of Natural Dye by Women in Hundihopo Village,

East Rote District, Rote Ndao District in August - October 2017. The
method used in this research was exploration and documentation.
Identification of plants was done by referring to Flora's book (Steenis,
2005). Data collection techniques used in this study were interviews.
Respondents were determined using a combination of purposive
sampling and snowball sampling techniques (Bernard, 2004).
Respondents were chiefs and craftsmen. The data were analyzed
descriptively qualitative.
The result of the research shown that there were 22 types of plants and
materials for natural coloring. The dye used was Tarum (Indigofera
tinctorea L) Mengkudu(Morinda citrifolia L) Kunyit (Curcuma
domestica L) Lagundi (Vitex trifolia L) Ketapang (Terminalia catappa)
Jati (Tectona grandis L.f) Kelumpang (Sterculia foetida L) Pinang
(Areca catechu) Trengguli (Cassia fistula L) Kecubung (Datura metel),
Biduri (Calotropis gigantae), Gadung (Dioscorea hispida), Kesambi
(Schleichera oleosa) Tene, Kame, Deras, falitiKelumpang (Sterculia
foetida L), memea, Lilin, Beura dan Kapur siri. The colors formed
from this material were black, red and yellow. Plant-based weaving
coloring was played by housewives. The results of this study also
shown that the role of mothers in sustaining household life was very
significant because the handicrafts could be sold at high prices.

Keywords : Ethnobotany, colors, Natural Dye, plants, Hundihopo

Village

55
 THE ANALYSIS OF QUANTITATIVE SEMI ACTIVE
COMPONENTS EXTRACT OF GORGONIAN SEA PLUMES
(Rumphella sp and Hicksonella sp) USES THIN LAYER
CHROMATOGRAPHY-DENSITOMETRY METHOD

Yunialdi Hapynes Teffu1*, Ruddy Suwandi2, Nurjanah3

1
Technology of Fishery Products Faculty of Fisheries and Marine
Sciences, Artha Wacana Christian University of Kupang-East Nusa
Tenggara
2,3
Lecturer Department of Aquatic Product Technology, Faculty of
Fisheries and Marine Sciences Bogor Agricultural University,
Lingkar Akademik Street, Campus IPB, Bogor 16680
Phone: 0251-8622915; Fax: 0251-8622916
Corresponding author: naldihapynes@gmail.com

Gorgonian sea plume is widely used by coastal communities in Eastern

Indonesia as an accessory such as bracelet and it is believed as a
traditional medicine to cure the rheumatic diseases. Some of the
previous studies have revealed the active component of gorgonian sea
plumes which indicates the potential biological activity to conduct the
further research.
The aim of this research was to analyze the semi quantitative active
component in the gorgonian sea plumes extract which based on
retention factor value (Rf). The determination of crude extract content
of gorgonian sea plumes (Rumphella sp and Hicksonella sp) usesd the
method of KLT-densitometry and it is compared to hydrate the
standard for flavonoids; boldin for alkaloids; digoxin for saponins; b-
sitosterol standard for steroids/triterpenoids.
The results based on retention factors value (Rf) showed that the crude
extract of gorgonian sea plumes (Rumphella sp and Hicksonella sp) for
alkaloid active compounds (Rf 0.50) and flavonoids (0.84) skin
methanol extract having Rf similar to the boldin standard (Rf 0.49) and
standard hydrate routine (0.85). Rf value of crude extract of gorgonian
sea plumes (Rumphella sp and Hicksonella sp) for the
steroid/triterpenoid active components of methanol skin extract (Rf
0.37), the extract of ethyl acetate skin (Rf 0.35) and n-Hexane skin (Rf

56
0.34) and standard b-sitosterol (0.33). Rf value of saponin active
component for skin methanol extract, the extract of ethyl acetate skin
and n-hexane leather extract haved is equal Rf value with standard
value of digoxin which is 0.97. The Rf value for all gorgonian sea
plumes extracts have the similar Rf value to the standard.

Keywords: Gorgonian, Hicksonella, Rumphella, Sea Plumes

57
 UTILIZATION OF LOCAL ISOLATE ENTOMOPATOGEN
FUNGUS TO CONTROL
Cylas formicarius FABRICUS IN SWEET POTATO

Nina Jeni Lapinangga1), Chatlynbi T. Br. Pandjaitan1), Jaqcualine A.

Bunga1), Jimrift H. H. Sonbai1),Yosefus F. Da Lopez2)
1
State Agricultural Polytechnic Kupang, Department of Crops and
Horticulture, East Nusa Tenggara Province, Indonesia.
2)
State Agricultural Polytechnic Kupang, Department of Dryland
Agriculture, East Nusa Tenggara Province, Indonesia

email: ninalapinangga@yahoo.co.id

Cylas formicarius is one of the destructive pests of sweet potatoes. One

of the techniques of biological pest control is by using
entomopathogenic fungi. The effectiveness of biological control by
using entomopathogenic fungi is determined by its adaptability to the
environment in which entomopathogenic fungi is applied. Biological
agents obtained from local sites will have higher adaptation and
performance in pests to be controlled than biological agents introduced
from other regions.
This study aims to explore, isolation and selection of local pathogenic
entomopathogenic fungi as biological agents to control Cylas
formicarius. Isolates used in the study came from around banana tree
crops, forest edges, and grasslands. Isolates were tested on three instar
larvae of Cylas formicarius. The isolate application was performed by
dripping the suspension of entomopathogenic fungus with a density of
1x106 conidia ml -1 topically on the test insect and repeated five times.
Exploratory results found that two types of local entomopatogenic
fungi isolate namely Metarhizium anisopliae and Beauveria bassiana.
The mortality of Cylas formicarius larvae in both fungi were 86.5%
and 85%, respectively. The results showed that the death of C.
formicarius larvae affected by B. bassiana treatment occurred five
days after the treatment but the peak of death on eight days after
treatment while M. anisopliae treatmen caused the death of C.
formicarius larvae has begun on three days after treatment and reached
58
the peak on the seventh day. Both types of fungi are potential to be
developed as bio pesticides to control C. formicarius pests.

Keywords: entomopathogenic fungi, Metarhizium anisopliae,

Beauveria bassiana, Cylas formicarius, biological
control.

59
INTEGRATING LOCAL MUSIC, CRAFT AND GAMES INTO
THE MATHEMATICS CURRICULUM IN TIMOR-LESTE:
HOW TEACHERS AND STUDENTS ARE MOTIVATED

DomingasXimenes Belo
Instituto Católico Para a Formação De Professores Diocese de
Baucau, Timor-Leste

This paper presents the experience of one Mathematics teacher of

integrating local knowledge into the Mathematics curriculum for
primary schools in Timor Leste. From independences in 1999 until the
present, the education system has continued to develop in order to help
teachers to be more effective in the teaching and learning process.
Student centred learning is one of the innovations that the government
has introduced in order to help develop the active thinking and creative
skills of both teachers and students in the classroom.
This paper provides the way in which ICFP has enabled new teachers
to provide local knowledge as a teaching resource at all levels of
education. Not only are the children were motivated, to want to learn
but also the teachers are encouraged to develop their own ability to
provide student centred learning in their lessons.

Keywords: local knowledge, teacher pre-service education, teaching

activities for mathematics education.

60
 EXPLORATION STUDY OF THE PIGMENT TYPES
OF CRAB STONE Grapsusalbolineatus
AS PHARMACEUTICAL AND COSMETICS
MATERIALS

Darus Saadah J. Paransa1*,, Desy M H. Mantiri1,James

J.H. Paulus1, Cysca Lumenta1, Medy ompie1, Suria
Darwisito1, Alfread Kase2.
1
Fisheries and Marine Science Study Program of Sam
Ratulangi University, Manado, North Sulawesi
2
Fisheries and Marine Science Study Program of Artha
Christian University, Kupang, East Nusa Tenggara.
*e-mail : darusparansa@unsrat.ac.id

Grapsusalbolineatus is a type of crustacean on the coastal of rocky

tropical regions like Indonesia. Some of the distribution areas of this
crab is the rocky coastline of, Tanawangko and Manggatasik Village
of, Manado City, North Sulawesi. Grapsusalbolineatus is known to
have a high content of carotenoid pigment. Carotenoid pigments are
useful for human needs, such as immune substances, and various
industrial raw materials such as pharmaceutical, cosmetic and
foodstuff industries. In this study identification of the pigment type
was performed on the carapace, gonad, blood, epidermal layer, and
hepatopancreas, using Thin Layer Chromatography method. The
identification of pigments on the carapace using the method of Column
Chromatography and High Performance Liquid Chromatography
(HPLC).
The results showed that Grapsusalbolineatus was identified at the
molting stage D.Thin Layer Chromatography Analyzes found pigment
types of β-Carotene, Ekinenon, Kantaksantin, Cryptosantin,
Adonirubin type, Astaxantin, Astacen. While the separation by
Column Chromatography method shows the results of pigments such
as β-Carotene, Cantaksantin, Adonirubin, and Fukosantin.
The concentration of pigment on carapace was 6,88 mg/gr, epidermal
layer 210,47 mg/gr, hepatopancreas 454,0 mg/gr, gonad 433,33 mg/gr,
and blood 753 mg/gr. The pigment content of each organ G.

61
albolineatus, ie at 6.95 mg carapace, epidermal layer 7,26 mg,
hepatopancreas 7.07 mg, gonad 34,94 mg, and blood 5,86 mg.

Keywords: Grapsusalbolineatus, Thin Layer Chromatography,

Column Chromatography, pigment

62
PROTEIN, AMINO ACIDS AND ORGANOLEPTIC QUALITY
OF SARDINE BAKASANG WITH PINEAPPLE PUREE
INTRODUCTION

Ovie Ningsih1, Umbu P.L. Dawa1, Yulian Abdullah2

1
Fisheries and Marine Science Faculty, ArthaWacana Christian
University, Kupang, East Nusa Tenggara Province
2
Agriculture Polytechnic State Kupang, East Nusa Tenggara
Province

Corresponding author: ovieningsih@gmail.com

Bakasangas one of traditional fermentation product from East

Indonesia. Usually, bakasang production need 30-45 days. Addition
bromalin enzyme resources, pineapple, could be expected to accelerate
the fermentation process with end product favoured by consumer.
The aim research is analysis of protein, amino acids (histidine, L-
leucine, glutamic acid) and organoleptic to Sardinella fish bakasang
with introduction pineapple puree.
The research result is was bakasang have 13% protein, 4563-6045 ppm
histidine, 6841-10275 ppm L-leucine, 5267-18358 ppm glutamic acid.
Consumer favoured of colour, flavour, taste level on 6-7 point as
preference value. Bakasang with combination 20% salts, 20% unripe
pineapple puree and 20 days fermentation at sardine fish puree 100%
as the best treatment.

Keywords: pineapple puree, bakasang, protein, amino acids,

organoleptic

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