Professional Documents
Culture Documents
tRNA - transfer RNA is a small RNA that has a very specific secondary
and tertiary structure such that it can bind an amino acid at one end, and
mRNA at the other end. It acts as an adaptor to carry the amino acid
elements of a protein to the appropriate place as coded for by the mRNA.
How does an mRNA specify amino acid sequence? The answer lies in
the genetic code. It would be impossible for each amino acid to be specified
by one nucleotide, because there are only 4 nucleotides and 20 amino acids.
Similarly, two nucleotide combinations could only specify 16 amino acids.
The final conclusion is that each amino acid is specified by a particular
combination of three nucleotides, called a codon, thus there are 64 codons in
total. Deciphering these triplet codes will answer the questions such as 1)
which codons specify each of the amino acids?, 2) how many of the 64
possible triplet codons are used? and 3) how are they used?
The main features of the genetic code were worked out during the
1960s. They include
The genetic code is composed of nucleotide triplets: Three
nucleotides in mRNA specify one amino acid in the poylpetide
product; thus each codon contains three nuecleotides.
The genetic code is degenarte: All but two of the amino acids are
specified by more than one codon.
The genetic code is ordered: Multiple codons for a given amino acid
and codons for amino acids with similar chemical properties are
closely related, usually differing by a single nucleotide.
The genetic code contains start and stop codons: Specific codons are
used to intiate and to terminate poypeptide chains.
U C A G
UUU Phenylalanine UCU Serine UAU Tyrosine UGU Cysteine
U
(Phe) (Ser) (Tyr) (Cys)
UUC Phe UCC Ser UAC Tyr UGC Cys C
UAA STOP UGA STOP
U UUA Leucine (Leu) UCA Ser A
(Ochre) (Opal)
UGG
UAG STOP
UUG Leu UCG Ser Tryptophan G
(Amber)
(Trp)
CCU Proline CAU Histidine CGU Arginine
CUU Leucine (Leu) U
(Pro) (His) (Arg)
CUC Leu CCC Pro CAC His CGC Arg C
C
CAA Glutamine
CUA Leu CCA Pro CGA Arg A
(Gln)
CUG Leu CCG Pro CAG Gln CGG Arg G
ACU AAU
AGU Serine
AUU Isoleucine (Ile) Threonine Asparagine U
(Ser)
(Thr) (Asn)
AUC Ile ACC Thr AAC Asn AGC Ser C
A
AAA Lysine AGA Arginine
AUA Ile ACA Thr A
(Lys) (Arg)
AUG Methionine
ACG Thr AAG Lys AGG Arg G
(Met) or START
GCU Alanine GAU Aspartic GGU Glycine
GUU Valine Val U
(Ala) acid (Asp) (Gly)
GUC (Val) GCC Ala GAC Asp GGC Gly C
G
GAA Glutamic
GUA Val GCA Ala GGA Gly A
acid (Glu)
GUG Val GCG Ala GAG Glu GGG Gly G
The tRNA molecules are clover leaf shaped. There are four major
arms, named for their structure and function. The acceptor arm consists of a
base-paired stem that ends in an unpaired sequence whose free 2’ – or 3’ –
OH group is aminoacylated. The other arms consist of base-paired stems
and unpaired loops. The TC arm is named for the presence of this triplet
sequence, the anticodon arm always contains the anticodon triplet in the
centre of the loop, and the D arm is named for its content of the base
dihydrouridine ( stands for pseudouridine and D stands for
dihydrouridine). The overall length of tRNA lengths is from 74 to 95 bases.
The tRNA are considered as adapter molecules since they carry amino acids.
There are from one to four tRNAs for each of the 20 amino acids.
The ribosomes contain binding sites for mRNA, for tRNAs and for a
variety of protein factors.
Ribosomes are also the site of a catalytic activity that is probably responsible
for the formation of the peptide bond during protein synthesis and have
several active centers. Each ribosome has two domains: translational
domain and exit domain. The traslational domain has aminoacyl site (A
sites) and peptidyl site (P Site) and the exit domain has exit site (E site).
Translation
Initiation
Prokaryotes Eukaryotes
The initiation of translation is in The initiation of translation is
prokaryotes, involves three initiation more complex in eukaryotes,
factors. involving several soluble initiation
factors.
The aminogroup of the methionine The aminogroup of the methionine
on the initiator tRNA is formylated. on the initiator tRNA is not
formylated.
The initiation complex forms at the The initiation complex forms at
Shine-Dalgarno sequence the 5’terminus of the mRNA.
(AGGAGG).
Initiation complex scans the mRNA, Initiation complex scans the
starting at Shine-Dalgarno sequence, mRNA, starting at 5’end,
searching for AUG translation- searching for AUG translation-
initiation codon. initiation codon.
5’ terminus of the mRNA is free of 7- A cap binding protein (CBP) binds
methyl guanosine cap to the 7-methyl guanosine cap at
the 5’ terminus of the mRNA.
In prokaryotes, there two release In eukaryotes, a single release
factors, RF-1 and Rf-2. Rf-1 factor (eRF) recognizes all three
recognizes termination codons UAA termination codons.
and UAG; RF-2 recognizes UAA and
UGA.