2. Microscopic agglutination caused by CAIHA: answers: presence of cold antibodies & infection w/ Mycoplasma pneumoniae
3. Mycoplasma pneumo causes walking pneumonia
4. Latex agglutination staph aureus – clumping & protein A 5. False DECREASE ESR – delay 8 hrs in set up 6. Prolonged apnea – pseudocholinesterase 7. Specimen rotavirus – stool 8. Specimen legionella – urine antigen 9. Cushing’s syndrome – hyperglycemia 10. Increased Ca and normal PTH – metastatic carcinoma 11. Primedone – Phenobarbital 12. Low sodium – hyperglycemia 13. Low sodium – repeat ion selective electrode 14. Low erythropoietin – polycythemia vera 15. PT normal (patient for gall bladder surgery), PTT prolonged, TT normal – factor XII assay 16. They give Ab panel and you identify (lewis Ab) – adsorbed from plasma 17. Cbc result: about method 1, method 2 – lyse resistant in Hgb C 18. quantitative fecal fat test – weight & extraction 19. absent trophozoite / merozoite – PLASMODIUM FALCIPARUM 20. lupus anticoagulant – thrombosis 21. UA results: 25 – 30 renal tubular epi cells acute tubular necrosis 22. Bacteria LAP (-), bile esculin (+), NaCl (growth), PYR (-) Resistant to vancomycin – leuconostoc 23. Carbon dioxide ion selective electrode measure – pCO2 24. ANA patterned picture – (speckled or nuclear anti-SSA) 25. Monocytosis seen in tuberculosis 26. FBS:120, OGTT: 140 – impaired glucose 27. Patient with fasting blood glucose 155mg/dL & random 225 mg/dL - OGTT 28. Hair perforation test: trichophyton mentagropytes & T. rubrum 29. 18% retics – Heinz body stain 30. 0.1% retics – pure red cell aplasia 31. streptokinase therapy does not work in myocardial infarction – D-dimer positive 32. lesion of arm, cigar bodies – sporothrix schoenkii 33. hct 33%, hct 33.5% in manual – lipemic 34. rbc in reagent strip, none seen in microscope: diluted ALKALINE urine 35. blastoconidia – mother & daughter cells 36. CSF storage in subsequent culture – incubate at 35C temp 37. TSI, A/A, oxidase positive – aeromonas 38. Pink colony on MAC agar, LOA -++: enterobacter cloacae 39. CA 19-9: pancreatic marker 40. Increased hemolytic anemia – increased UNCONJUGATED bili, increased urobilinogen 41. EIA HTLA ½ reactive, what to do next? – western blot 42. False NEGATIVE ABO – red cells positive DAT 43. Anti-IgG NEGATIVE, anti C3D POSITIVE – prewarm saline solution 44. Echinocytes picture – faulty to dry the slide 45. No growth @ 6.5% NaCl – streptococcus bovis (group D), endocarditis & colorectal cancer 46. Specific gravity 1.010 at 4C result glucose 1000mg/dL – correct the temperature due to high glucose 47. What is the saliva Le(A+ B-) person? – Lea 48. Ph 4.5 in urine – high protein diet 49. Tap water: M. gordonae 50. Mutation of polycythemia vera: JAK 51. Pharyngitis, seen in renal biopsy: s. pyogenes 52. Differentiate p. aeruginosa from p. putida: 42C 53. Valinomycin: potassium 54. Image of crithidia lucilae: double stranded, SLE 55. pH measurement needs: known buffer @ constant temp 56. butchers cut – e. rhusiopathiae 57. many tear drops(PBS), what deficiency? – DNA 58. favors growth of anaerobic GNR – vitamin K & hemin 59. rapid testing for CMV? - PCR DNA urine 60. rouleaux not seen in what phase? AHG 61. Burr cells – uremia 62. Newborn w/o thymus gland, normal B cells but no production of t cells – DiGeorge syndrome 63. PT control out but APTT within normal range – change CaCl2 reagent 64. Organism isolated in Hektoen: TSI K/A, H2S (+), PAD (-), lysine decarboxylase (-), urea(+), citrate (+) tech report as NORMAL FLORA 65. Instrument linearity something about comparing means – paired T-test 66. Postprandial lipemia? Triglycerides!!! 67. Whole blood donation stops at 390 mL: pRBC (low volume unit) 68. Le(a) Le(b) IS 37 AHG 0+1+00 0+1+00 + 0 0 + / - 2W + / - 2W + 0 0 +/ -2W +/-2W glycolipid absorbed from plasma 69. Ab that deteriorates in storage: P1 70. Choose positive controls to test for anti-c and negative control to test anti-Fy(a): C+c+ for positive control, Fy(a) for negative control 71. Detection of Ab where 11 tubes resulted NEGATIVE in AHG, but when added CC 4 of them didn’t agglutinate – machine didn’t dispense correctly the saline in the wash 72. IS 37 AHG CC SC1 0 0 0 2+ SC2 +/- +/- 0 2+ Answer: add 4 drops of serum 73. patient DAT (4+), IAT (+), did eluate and the results are DAT (2+) they auto absorb serum and keeps reacting to SCI1 & SC2 in AHG, what should you do? Panel cells (there was also enzyme panel cells, report DAT or make another autoadsorption) 74. Anti-A Anti-B Rh Du Control D 0 0 3+ + - IS 37 AHG CC SC1 0 0 0 2+ SC2 0 0 0 2+ Patient cells 0, 0, 2+ not tested presents auto allo Ab 75. calculate % of saturation – UIBC 185, Fe 125, TIBC = 185 + 125 = 310 %sat (125/310) * 100 = 40% 76. PT normal, PTT (56), mix 1: plasma (47) factor VIII deficiency 77. Sample taken from indwelling catheter, patient isn’t on anticoagulants yet PTT & TT are way elevated – HEPARIN CONTAMINATION (from catheter) 78. In the second phase of platelet aggregation what is irreversible? Fibrin formation 79. Control and patient’s PTT elevated, control & patient PT elevated: thromboplastin was added by error 80. Anti-A Anti-B A B 4+ 4+ 2+ 2+ What should tech do? First, perform Ab screen w/ autocontrol. If screen & autocontrol = negative THEN Prewarm b/c cold agglutinins 180. Anti-A Anti-B A B 0 2+mf 4+ 0 Discrepancy due to Bx-subgroup 81. HgbA1C – what can be the trouble with the test??? decreased life span on RBCs (in the case of sickle cell) 82. Mycoplasma can’t be treated w/ penicillin = no cell wall 83. Effect of dextran as anticoagulant: destroy D antigen 84. Potassium permanganate: quenching agent 85. Common error in PCR: nucleic acid contamination 86. Low incidence Ag present in blood panel: Wra 87. Surfactant fetal lung maturity – phosphatidyl glycerol 88. Anti-microsomal – hashimoto’s thyroiditis 89. In multichannel analyzer, controls of enzymatic assays are lower than expected values while non-enzymatic assay controls are within normal limits. What is the probable cause? instrument temperature may be low 90. Speckled pattern – anti SBB, anti RNP, anti Sm 91. Patient has the results after collecting blood in an indwelling catheter. Patient is not in heparin / anticoagulant therapy. APTT: abnormal, PT: normal, fibrinogen: 150 mg/dL, what test should be ordered? Factor XII assay 92. PREDOMINANTLY seen in acute phase of infection but rarely seen in chronic infection? anti- HBc, IgM 93. Adrenal cushing syndrome – TSH decrease, cortisol increase 94. Deferred donor: Hepatitis Ig six months ago