enzyme volume (µreaction rate (1/s)

200 0.01014
150 0.008649
100 0.006676 0.012
50 0.003954
0 -0.0000164 f(x) = 5.00156E-05x +
0.01 R² = 0.9632413241

substrate concentreaction rate (1/s)

0.008
20 0.01174
15 0.01028
101 0.009268

reaction rate (1/s)

0.006
5 0.006122
2.5 0.003704
1 0.00168 0.004

0.002

0
0 50

-0.002

SUMMARY OUTPUT

Regression Statistics
Multiple R 0.9814485845
R Square 0.9632413241
Adjusted R Squar 0.9509884321
Standard Error 17.5020655751
Observations 5

ANOVA
df SS MS F Significance F
Regression 1 24081.03310182 24081.03310182 78.6133858012 0.0030247342
Residual 3 918.9668981841 306.3222993947
Total 4 25000

Coefficients Standard Error t Stat P-value Lower 95%

Intercept -13.2518628395 14.9805586708 -0.8846040479 3 -60.9266864324
X Variable 1 19258.81773032 2172.107785614 8.8664189954 0.0030247342 12346.20133332
0.012

f(x) = 5.00156E-05x + 0.00087896

0.01 R² = 0.9632413241

0.008

0.006

0.004 reaction rate (1/s)

Linear (reaction rate (1/s))

0.002

0
0 50 100 150 200 250

-0.002

enzyme volume (µl)

 Upper 95% Lower 95.0% Upper 95.0%
34.4229607534 -60.9266864324 34.4229607534
26171.43412733 12346.20133332 26171.43412733
reaction rate (1/s)
Linear (reaction rate (1/s))