National Public Health Laboratory Test Handbook 2018 FINAL (28082018)

THE NATIONAL PUBLIC
HEALTH LABORATORY
TEST HANDBOOK 2018
1st Edition
NPHL, Ministry of Health Malaysia

First Printing, 2018
Copyright National Public Health Laboratory (NPHL) 2018
All right reserved. No part of this publication may be reproduced or transmitted in any form or
by any means, electronic or mechanical, including photocopy, recording, or any information
storage and retrieval system, without permission in writing from National Public Health
Laboratory.
Published in Malaysia by
NATIONAL PUBLIC HEALTH LABORATORY (NPHL)
MINISTRY OF HEALTH MALAYSIA
LOT 1853 KAMPUNG MELAYU
47000 SUNGAI BULOH
SELANGOR DARUL EHSAN
Perpustakaan Negara Malaysia Cataloguing-in-Publication Data
THE NATIONAL PUBLIC HEALTH LABORATORY TEST HANDBOOK 2018/

Editor in Chief Dr Noorliza Mohamad Noordin;
Editor Khairul Azan Hashim, Selvanesan Sengol, Zirwatul Adilah Aziz, Noor Hamisson Abas,
Sharifah Milkah Syed Abd Rahman, Norizah Ismail, Wan Noraini Wan Yussof, Hannah Phoon Yik
Phing, Dr Zulhainan Hamzah, Sharmili Kuppan, Priscilla Dinang Anak Kasim
ISBN 978-967-13278-1-4
1.Public Health Laboratories—Malaysia—Handbooks, manuals, etc
2.Public Health—Malaysia—Handbooks, manuals, etc
I. Noorliza Mohamad Noordin, Dr
II. Khairul Azan Hashim III. Selvanesan Sengol
IV. Zirwatul Adilah Aziz V. Noor Hamisson Abas VI. Sharifah Milkah Syed Abd Rahman
VII. Norizah Ismail VIII. Wan Noraini Wan Yussof IX. Hannah Phoon Yik Phing
X. Zulhainan Hamzah, Dr. XI. Sharmili Kuppan XII. Priscilla Dinang Anak Kasim
362.1
i
Deputy Director General of Public Health’s Foreword
The National Public Health Laboratory (NPHL) has made great strides and effort in preparing and
publishing the NPHL the Test Handbook 2018. This shows the commitment and desire of every
individual in the NPHL to achieve excellence by being relevant to the community in the production of
timely laboratory results, especially in outbreak investigations. The achievement of the objectives set
by the NPHL is of paramount importance for the advancement of the vision and mission of the Public
Health Sector, Ministry of Health Malaysia to ensure that Malaysians have the best public health
delivery system in the region.
I would like to take a moment to extend my heartiest congratulations to the NPHL Director, the
Chairman of the NPHL Test Handbook 2018, the NPHL Test Handbook Editorial Board and Taskforce
and all the contributors of this test handbook for this timely and handy guide. This test handbook will
serve as an important and comprehensive reference for all the public health field officers and many
referring laboratories in Malaysia that the right tests are ordered at the right time and transported in
the right manner. Therefore, the outcome will be quality results and reliable answers to the outbreak
investigations.
On that note, I hope to see that the NPHL Test Handbook 2018 being fully utilized and being made the
definitive reference for the Public Health Sector for years to come.
Thank you.
DATO' DR. HJ. AZMAN BIN HJ. ABU BAKAR

DEPUTY DIRECTOR GENERAL OF HEALTH (PUBLIC HEALTH)
ii
Director of Disease Control Division’s Foreword
The National Public Health Laboratory (NPHL) is spearheading the challenge to align all of the efforts by
the Public Health Sector to the goals and objectives set by the Ministry of Health Malaysia. In doing so,
the NPHL has produced and published a NPHL Test Handbook 2018 which describes in detail all the
necessary information needed for the field officers in the Public Health Sector.
The production of this handy test handbook is highly anticipated by all especially those in the frontlines
of outbreak investigations. The information provided in this test handbook is extremely necessary for
the ordering of tests where the results obtained can curb the spread of infectious diseases in the
community.
Hence, I highly encourage the relevant personnel involved in the frontlines of outbreak investigations
to read and to make full use of this test handbook so that the key findings of your investigations can be
determined early and accurately.
I hereby thank and congratulate the Director of NPHL, the Chairman of the NPHL Test Handbook 2018,
the NPHL Test Handbook Taskforce and the contributors of the handbook for the success and
production of this guide.
Thank you.
DATO' DR CHONG CHEE KHEONG

DIRECTOR OF DISEASE CONTROL DIVISION
iii
Director’s Preface
The National Public Health Laboratory (NPHL) is comprised of five key divisions: The Disease, Food,
Epidemiology, Quality and Administration Divisions. In accordance with the public health objectives of
the country, the NPHL is committed to provide analytical and diagnostic testing capacity while serving
as a reference centre for disease and food safety.
The quality and standard of the services offered by the NPHL is reflected in its assessments by the World
Health Organization (WHO) and the following accreditations by Standards Malaysia: MS ISO 9001:2008
(General), MS ISO 15189:2014 (Disease Division) and MS ISO 17025: 2005 (Food Division and Tar &
Nicotine Unit).
This test handbook is intended to serve as the latest reference to the tests offered by the NPHL and
supplants all previous versions. It includes comprehensive information on the tests offered, the suitable
type and collection of samples, appropriate containers types, transportation requirements and other
necessities for placing test requests. We have also included the information about the other Public
Health Laboratories in Malaysia which offer similar tests, such as, MKA Ipoh, MKA Johor Bahru, MKA
Kota Bharu and MKA Kota Kinabalu, for our users’ reference.
If this handbook does not address any queries that you may have, we welcome you to contact us with
your questions. The latest version of this handbook and its following revisions can be accessed through
the NPHL’s official web address, http://mkak.moh.gov.my/ms/.
Thank you,
DR. HANI BINTI MAT HUSSIN

ADVISOR OF THE NPHL TEST HANDBOOK 2018
DIRECTOR
NATIONAL PUBLIC HEALTH LABORATORY
iv
Chairman’s Preface
Since the establishment of the NPHL, few service handbooks have been published to provide clients
with the necessary information related to its services. The services described in these handbooks not
only included the tests offered but also other supplementary efforts such as quality and training
activities. However, The National Public Health Laboratory Test Handbook 2018 is the first test
handbook we have produced and is designed to function as the reference for all the tests offered by
the NPHL.
In view of the feedback and comments received from clients, we embark to create a test handbook that
specifically focuses on the tests offered by the NPHL and other public health laboratories such as MKA
Ipoh, MKA Johor Bahru, MKA Kota Bharu and MKA Kota Kinabalu. We believe that this test handbook
will function as a handy reference and guide to clients in placing test requests.
This handbook will help the key users of this handbook such as the public health medical officers, Family
Medicine Specialists, and other field officers during an outbreak to find the pertinent information that
they need when ordering tests. This test handbook answers the How, What, Where, When, and Why
questions related to test ordering. For instance, “What specimen is the correct specimen? Which is the
potential pathogen to test for in an outbreak? How do we collect a specimen? And who do we contact?”.
The chapters in this book are arranged in sequence from the syndromic approach leading to the
possible list of pathogens which will take users to the comprehensive lists of tests offered by the NPHL
Disease Division and Food Division. The tests in each of these categories are organized alphabetically
into tables according to the pathogens or parameters tested. The tests listed are further described by
the method of testing, sample type, sample collection, sample volume, sample containers,
transportation requirements, type of request form, cost per test and frequency of testing by the NPHL
and the NPHL’s subsidiary laboratories that offer them. The remarks column indicates the tests that
require prior approval from the NPHL and is followed by the details of the testing unit that can be
contacted for such appointments. The contents of the comprehensive lists of tests are very important
and users should pay attention to every detail in the columns for the information that they need.
v
We have also included a chapter on the procedure for specimen collection and transportation to assist
first-time specimen collectors on the right methods to follow when collecting specimens. The tracing
of results for the tests ordered can also be found in the proceeding chapters to enable the test
requestors to trace their test results.
In keeping with our intention to assist users’ using this book at the field, we conducted a Benchtop
Outbreak Simulation Exercise for Pre-testing of the NPHL Test Handbook 2018, an outbreak simulation
exercise with real case studies among the stakeholders that tested the comprehensiveness of the book
to determine the user-friendliness, ease-of-navigation, completeness, handiness and resourcefulness
of the NPHL Test Handbook. I would like to take this opportunity to extend my gratitude for the
significant contributions from officers of The Disease Control Division, Petaling District Health Office,
Lembah Pantai District Health office, Titiwangsa District Health Office, Klang District Health Office,
Gombak District Health Office and the contributions of Dr Rosemawati Ariffin, Panelist of the benchtop
exercise. A special thanks to Dr Zainudin Abdul Wahab, Director of JKWPKLP and Dato’ Dr Khalid
Ibrahim, Director of JKNS for their continuous support and to all contributors of the test handbook. Our
success is owed to your contributions and efforts.
We hope that this book will serve its purpose as a complete and handy guide to our clients. This book
also is a work in progress and hence, we encourage you to contact us with any queries, comments or
ideas that we may employ in our continuous effort in providing our best service. It is my hope and
expectation that this book will be a referenced resource for all health professionals leading to improved
preparedness, prevention and control of infectious disease in our country.
Thank you,
DR HAJAH NOORLIZA BT MOHAMAD NOORDIN

CHAIRMAN OF NPHL TEST HANDBOOK 2018
HEAD OF DISEASE DIVISION
NATIONAL PUBLIC HEALTH LABORATORY
vi
EDITORIAL BOARD
EDITORIAL BOARD MEMBERS
1. DR. NOORLIZA BINTI MOHAMAD NOORDIN---------------------------Editor in Chief

2. KHAIRUL AZAN B HASHIM
3. SELVANESAN A/L SENGOL
4. ZIRWATUL ADILAH BT AZIZ
5. NOOR HAMISSON BINTI ABAS
6. SHARIFAH MILKAH BT SYED ABD RAHMAN
7. NORIZAH BT ISMAIL
8. WAN NORAINI WAN YUSSOF
9. HANNAH PHOON YIK PHING
10. DR ZULHAINAN BIN HAMZAH (not in the photo)
11. SHARMILI KUPPAN (not in the photo)
12. PRISCILLA DINANG ANAK KASIM (not in the photo)
KINDLY CONTACT US FOR ANY ENQUIRIES, SUGGESTIONS FOR FUTURE IMPROVEMENTS AND COMMENTS.
EMAIL: hannah_phoon@moh.gov.my
TEL: 03-6126 1200 ext 1317/ 4048
vii
THE NATIONAL PUBLIC HEALTH LABORATORY TEST HANDBOOK 2018 TASK FORCE MEMBERS
ADVISOR
DR HANI BT MAT HUSIN
DIRECTOR, NPHL
CHAIRMAN
COORDINATOR
DR NOORLIZA BT NOORDIN
NORIZAH BT ISMAIL
HEAD OF DISEASE DIVISION, NPHL
GROUP I GROUP II GROUP III GROUP IV GROUP V

MICROBIOLOGY FOOD BIOCHEMISTRY ENTOMOLOGY SAMPLE COLLECTION
GROUP LEADER GROUP LEADER GROUP LEADER GROUP LEADER

GROUP LEADER
WAN NORAINI WAN NOOR HAMISSON BT SHARIFAH MILKAH BT SELVANESON SENGOL
SHARMILI KUPPAN
YUSOF ABBAS SYED ABD RAHMAN
viii
Table of Contents
DEPUTY DIRECTOR GENERAL OF PUBLIC HEALTH’S FOREWORD ............................................................................ i
DIRECTOR OF DISEASE CONTROL DIVISION’S FOREWORD .................................................................................... iii
DIRECTOR’S PREFACE............................................................................................................................................. iv
CHAIRMAN’S PREFACE ............................................................................................................................................ v
EDITORIAL BOARD ................................................................................................................................................ vii
THE NATIONAL PUBLIC HEALTH LABORATORY TEST HANDBOOK 2018 TASK FORCE MEMBERS ........................ viii
INTRODUCTION ...................................................................................................................................................... 3
OBJECTIVES OF THE NPHL 2018 TEST HANDBOOK ................................................................................................. 4
QUICK GUIDE OF POSSIBLE PATHOGENS BY ROUTES OF TRANSMISSION .............................................................. 5
COMPREHENSIVE TEST DIRECTORIES ..................................................................................................................... 8
A. THE COMPREHENSIVE DIRECTORY OF INFECTIOUS DISEASE TESTS IN NPHL ....................................... 8
B. THE COMPREHENSIVE LIST OF BIOCHEMISTRY TESTS IN NPHL ........................................................ 135
C. THE COMPREHENSIVE LIST OF FOOD DIVISION TESTS IN NPHL ....................................................... 144
1. MICROBIOLOGY ................................................................................................................................. 144
2. MOLECULAR ...................................................................................................................................... 156
3. GMO .................................................................................................................................................. 166
4. MYCOTOXIN ...................................................................................................................................... 175
5. INDUSTRIAL POLLUTION AND FOOD ENVIRONMENT (PENCEMARAN INDUSTRI DAN PERSEKITARAN
MAKANAN, PIPM) ............................................................................................................................. 177
6. NATURAL CONTAMINATION (PENCEMARAN SEMULAJADI) ............................................................. 182
7. DRUG RESIDUE .................................................................................................................................. 187
8. PESTICIDE RESIDUE ........................................................................................................................... 196
9. SPP..................................................................................................................................................... 207
10. ADDITIVE ........................................................................................................................................... 212
D. THE COMPREHENSIVE LIST OF ENTOMOLOGY TESTS IN NPHL ......................................................... 220
SPECIMEN COLLECTION PROCEDURES FOR DISEASE DIVISION LABORATORY TESTING ..................................... 231
A. CLINICAL SPECIMEN .......................................................................................................................... 231
1. GENERAL RULES ................................................................................................................................ 231
2. SERUM ............................................................................................................................................... 231
3. RESPIRATORY SPECIMEN ................................................................................................................... 231
4. BODY FLUIDS ..................................................................................................................................... 232
5. URINE AND STOOL/RECTAL SWAB .................................................................................................... 233
6. EYE/EAR/GENITAL/PUS SWAB ........................................................................................................... 233
7. SKIN BIOPSY/LESIONS/VESICLE SWABS ............................................................................................. 234
B. ENVIRONMENTAL AND NON CLINICAL SPECIMEN ........................................................................... 235
1.0 UNTREATED WATER SAMPLING FOR LEPTOSPIRA ............................................................................ 235
2.0 SOIL SAMPLING FOR LEPTOSPIRA ..................................................................................................... 235
1
3.0 WATER SAMPLING FOR LEGIONELLA ................................................................................................ 235
4.0 SOIL SAMPLING FOR BURKHOLDERIA PSEUDOMALLEI ..................................................................... 236
5.0 WATER SAMPLE FOR BURKHOLDERIA PSEUDOMALLEI .................................................................... 236
6.0 CIGARETTES TESTING (NICOTINE, TAR & CARBON MONOXIDE) ....................................................... 236
C. SPECIMEN REJECTION CRITERIA ....................................................................................................... 237
A VISUAL GUIDE FOR INFECTIOUS DISEASE SAMPLE COLLECTION ................................................................. 238
SPECIMEN COLLECTION PROCEDURES FOR FOOD DIVISION LABORATORY TESTING ........................................ 241
A. SAMPLING PROCEDURE FOR MICROBIOLOGICAL ANALYSIS ............................................................ 241
1. UTENSILS AND STERILIZATION .......................................................................................................... 241
2. PROCEDURE FOR USING SAMPLING BAGS ........................................................................................ 241
3. QUALITY CONTROL OF SAMPLE DURING SAMPLING ........................................................................ 243
4. SWAB SAMPLING PROCEDURE.......................................................................................................... 243
5. LABELLING AND ‘LAK’ PROCEDURE ................................................................................................... 244
6. PROCEDURE FOR PACKING OF SAMPLES IN AN ICE BOX .................................................................. 245
B. SAMPLING PROCEDURE FOR CHEMICAL ANALYSIS .......................................................................... 246
1. GENERAL RULES ................................................................................................................................ 246
2. SPECIAL REQUIREMENT OF SAMPLING FOR PESTICIDES ANALYSIS .................................................. 246
3. SPECIAL REQUIREMENT OF SAMPLING FOR HEAVY METAL ANALYSIS ............................................. 246
4. SPECIAL REQUIREMENT OF SAMPLING FOR VETERINARY DRUG RESIDUE ANALYSIS ....................... 247
5. SPECIAL REQUIREMENT OF SAMPLING FOR STANDARD AND NUTRITIONAL LABELLING ANALYSIS. 247
FOOD DIVISION CLIENT CHARTER ....................................................................................................................... 248
NPHL OPERATING HOURS AND ON CALL CONTACT NUMBER ............................................................................ 251
RESULTS TRACING ............................................................................................................................................... 251
A. SIMKA (SISTEM INFORMASI MAKLUMAT KESIHATAN AWAM)......................................................... 251
B. TELEPHONE ....................................................................................................................................... 251
REFERENCES........................................................................................................................................................ 252
APPENDIX 1 BORANG PERMOHONAN UJIAN MAKMAL (SPESIMEN KLINIKAL) .................................................. 253
APPENDIX 2 PERMOHONAN UJIAN TB................................................................................................................ 254
APPENDIX 3 LABORATORY REQUEST FORM FOR DENGUE & FLAVIVIRUS ......................................................... 255
APPENDIX 4 BORANG PERMOHONAN UJIAN MAKMAL HFMD .......................................................................... 256
APPENDIX 5 MEASLES - BORANG PERMOHONAN DAN KEPUTUSAN UJIAN MAKMAL ....................................... 257
APPENDIX 6 BORANG PERMOHONAN PENYIASATAN/PEMANTAUAN SAMPEL PERSEKITARAN ........................ 258
APPENDIX 7 BORANG PERMOHONAN UJIAN PARAS CHOLINESTERASE ............................................................. 260
APPENDIX 8 CONGENITAL HYPOTHYROIDISM CORD BLOOD SCREENING TEST ................................................. 261
APPENDIX 9 PERMOHONAN UJIAN KERINTANGAN VEKTOR TERHADAP RACUN SERANGGA ............................ 262
APPENDIX 10 REQUEST FOR ANALYSIS OF FOOD SAMPLE ................................................................................. 264
LIST OF CONTRIBUTORS ...................................................................................................................................... 265
ACKNOWLEDGEMENT ........................................................................................................................................ 266
2
INTRODUCTION
The National Public Health Laboratory (NPHL) is comprised of five

key divisions: the Disease, Food, Epidemiology, Quality and
Administration divisions. The NPHL which reports directly to the
Disease Control Division in the Ministry of Health, Malaysia serves as
the Malaysian reference laboratory for disease and food testing. All
analytical and diagnostic testing done in the NPHL is performed to meet
the public health objectives of the country and to achieve the vision and
mission of being at the forefront of laboratory testing especially in
outbreaks for disease control and prevention.
Since the establishment of the NPHL, few service handbooks have
been published to provide clients with the necessary information
related to its services. The services described in these handbooks not
only included the tests offered but also the NPHL quality and training
activities. However, in view of the feedback and comments received
from the NPHL clients, a brand-new test handbook that specifically
focuses on the tests offered by the NPHL and the other public health
laboratories under the Ministry of Health in Malaysia was created.
The NPHL Test Handbook 2018 offers the latest and most updated
version of the tests offered and comprehensive but highly-relevant test
details for the reference of clients who collect samples in the field. This
effort was done to ensure the best possible information reaches the
clients so that the right samples are collected at the right time and sent
in the right conditions in order to obtain the right results. Ultimately,
optimal control and prevention of communicable diseases can be
achieved by the Ministry of Health, Malaysia.
3
OBJECTIVES OF THE NPHL 2018 TEST HANDBOOK
1. To align the content of the test handbook with the core

functions of the NPHL in achieving the public health objectives
of the country.
2. To be the latest, updated and comprehensive reference to all
the tests offered by the NPHL.
3. To function as a user-friendly, handy and easy-to-navigate
directory for NPHL clients that catalogues the different tests.
4. To be a definitive guide for NPHL clients to meet all the
requirements for test ordering and a directory to other
laboratories than the NPHL that offer related tests.
4
QUICK GUIDE OF POSSIBLE PATHOGENS BY ROUTES OF
TRANSMISSION
Infections according to Possible pathogens (in alphabetical Page number

transmission routes order)
RESPIRATORY INFECTIONS Adenovirus 9
Bordetella pertussis 14
Burkholderia cepacia 16, 104
Burkholderia pseudomallei 17, 104
Coronavirus (MERS CoV) 23
Corynebacterium diphtheriae 25
Ebola virus 31
Haemophilus influenzae 40, 104
Human herpesvirus 5 - (Cytomegalovirus) 45
Human metapneumovirus 61
Human parainfluenza virus 1 99
Human respiratory syncytial virus 106
Influenza A 48, 49, 51, 54
Influenza B 52, 54
Klebsiella pneumoniae 55, 104
Legionella pneumophilia 56
Measles virus 63
Moraxella catarrhalis 64, 104
Mumps virus 66
Mycobacterium tuberculosis 66
Mycoplasma pneumoniae 89
Neisseriae meningitidis 91, 104
Nipah virus 95
Non-tuberculosis mycobacterium (NTM) 96
species
Pseudomonas aeruginosa 102, 104
Rabies virus 103
Rhinovirus 107
Rubella virus 108
Staphylococcus aureus 104, 119
Stenotrophomonas maltophilia 104, 123
Streptococcus Group C 104, 124
Streptococcus Group G 104, 124
Streptococcus pneumoniae 104, 125
Streptococcus pyogenes 104, 124
VECTOR BORNE Dengue virus 29, 39
INFECTIONS Chikungunya 23
Page 5
Japanese encephalitis virus 54
Murray Valley encephalitis virus 39
St. Louis encephalitis virus 39
Tick-borne encephalitis virus 39
West Nile virus 132
Yellow fever virus 132
Zika virus 134
GASTROINTESTINAL TRACT Aeromonas sp. (caviee, hydrophilia, veronii) 10, 32

INFECTIONS Bacillus cereus 12, 32
Blastocystis hominis 21
Campylobacter jejunii 21, 32
Cryptosporidium parvum, hominis 29
Dientamoeba fragilis 30
Entamoeba histolytica 31
Enterovirus A (Human coxsackievirus A16) 28, 33
Enterovirus A (Human coxsackievirus A24) 33
Enterovirus A (Human coxsackievirus B) 33
Enterovirus A(Human echovirus 11) 33
Enterovirus A(human echovirus 9) 33
Enterovirus A(Human enterovirus 70) 33
Enterovirus A(Human enterovirus 71) 33, 35
Enterovirus C (Poliovirus 1) 33
Escherichia coli (Diarrheagenic) 32, 36
Giardia lamblia 39
Hepatitis A virus 43
Listeria monocytogenes 32, 59
Mamastro virus (Astrovirus) 12
Mammalian orthoreovirus 95
Norwalk virus (Norovirus) 97
Plesiomonas shigelloides 32, 101
Rotavirus A 109
Salmonella sp. (bongori & enterica) 32, 110
Salmonella Typhi 32, 110, 112
Quick Guide of Possible Pathogens by Routes of Transmission Page 6

Sapporo virus 115
Shigella sp. 32, 116
Staphylococcus aureus 32, 117
Vibrio cholerae/ Vibrio sp 32, 130
Yersinia enterocolitica 32, 133
CENTRAL NERVOUS Human herpesvirus 1 (HSV Type 1) 45, 46

SYSTEM INFECTIONS Human herpesvirus 2 (HSV Type 2) 46
Human herpesvirus 3 (Varicella Zoster) 45, 130
BLOOD BORNE INFECTIONS Brugia malayi 38

Brugia timori 38
Hepatitis B virus 44
Human immunodeficiency virus 1 48
Human immunodeficiency virus 2 48
Plasmodium falciparum 61
Plasmodium knowlesi 61
Plasmodium malariae 61
Plasmodium ovale 61
Plasmodium vivax 61
Wuchereria bancrofti 38
ZOONOTIC INFECTIONS Leptospira interrogans 57

Brucella sp., B.melintensis, B. abortus, B. suis 20
Burkholderia pseudomallei 17
Coxiella burnettii 27
SEXUALLY TRANSMITTED Neisseria gonorrhoeae 90

INFECTIONS Treponema pallidum 128
CUTANEOUS INFECTIONS Mycobacterium leprae 88
EMERGING AND SPECIAL Orthopoxvirus 98

PATHOGENS Parapoxvirus 99
Quick Guide of Possible Pathogens by Routes of Transmission Page 7

COMPREHENSIVE TEST DIRECTORIES
A. The Comprehensive Directory of Infectious Disease Tests in NPHL
IMPORTANT POINTS TO NOTE:

i. List of test abbreviations:
Abbreviations:
EM Electron Microscopy
IFAT Indirect immunoflouresence assay Test
PCR Polymerase Chain Reaction (conventional)
qPCR Quantitative or Realtime PCR
RAT Rapid Antigen test
RTK Rapid Test Kit
VI Virus Isolation
VTM Viral transport media
ii. The Cost Per Tests listed in this directory are NOT the actual payment charges for the tests. The costs included in this directory are for user reference and
understanding/appreciation of the costs involved to run tests which can be very expensive. For actual payment charges per tests, please refer to the PERINTAH FI
(PERUBATAN) (KOS PERKHIDMATAN) pua_20141231_P.U. (A) 363.
Page 8
Public Health
(Preference of sample
Sent either one only)
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
Pathogen Category Transport LTAT Remark(s)
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
Outbreak IFAT/ Sputum 1-3 ml Sterile 2-8°C √ 1. Sample should

Adenovirus (Respiratory)
MKAK-BPU-U01
EM - 2 days
qPCR - 3 days
IFAT - 7 days
EM-Daily
qPCR-Daily
IFAT-Daily
EM - RM 15.00
qPCR - RM210.00
IFAT - RM 35.00
Virology
Electron Microscopy Unit 1320 / 1335

Molecular Unit 1321
Virus Isolation Unit 1315/1325
qPCR / screw be collected within
EM capped 5 days after onset
container of illness.
Nasopha 1-3 ml Sterile
-ryngeal screw 2. A brief concise
Aspirate capped history of illness
container and physical
Tracheal 1-3 ml Sterile findings is required
Aspirate screw especially the date
capped of onset of illness
container and date of sample
Endo 1-3 ml Sterile collection.
-tracheal screw
tube capped
aspirate container
Broncho 1-3 ml Sterile
-alveolar screw
lavage capped
container
IFAT/ Throat - Sterile
qPCR / swab container
EM with 2.0-
2.5 ml of
VTM
Nasal - Sterile
Pharyn container
-geal with 2.0-
Swab 2.5 ml of
VTM
Nasal - Sterile
Swab container
with 2.0-
2.5 ml VTM
A. The Comprehensive Directory of Infectious Disease Tests in NPHL Page 9

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
Lung 1.5cm Sterile

tissue cube screw
(post- in a capped
mortem few container
case) drops
of
VTM
Outbreak RAT / Fresh 5 ml / Sterile 2-8°C √ 1. Sample should
Adenovirus (Enteric)
MKAK-BPU-U01
EM - 2 days
PCR - 5 days
RAT - 24 hours
EM-Daily
PCR-Daily
RAT -Daily
EM - RM 15.00
PCR-RM214.30
RAT -RM20.00
Virology

Molecular Unit 1321
Virus Isolation Unit 1315 / 1325
PCR/ stool 1.5 cm screw be collected within
EM cube capped 5 days after onset
PCR Rectal - Sterile
2. A brief concise
Swab container
history of illness
with 2.0-
and physical
2.5 ml of findings is required
VTM
especially the date
of onset of illness
and date of sample
collection.
Outbreak Culture & Fresh 3-5 Sterile 5 days Daily 1329/ √ √ √ √ √ 1. Please refer to
Aeromonas sp.
collection at 2°C – 8°C

Send within 2 hours after
MKAK-BPU-U01
RM 70.00
Bacteriology
Sensitivit stool gm container 1330 Specimen
y Collection
Procedures for
Disease Division
Laboratory
Testing.
2. A sample which
is received more
than 2 hours will
be rejected.

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
Fresh NA Cary Blair 5 days Daily 1329/ √ √ √ √ √ 1. Please refer to
unavoidable, send at 2°C – 8°C.

at ambient temperature. If delay is
Send within 24 hours after collection
MKAK-BPU-U01
RM 70.00
Bacteriology
stool 1330 Specimen
Collection
Procedures for
Disease Division
Laboratory
Testing.
2. Delay in
transportation will
affect the viability
of the bacteria.
Rectal NA Cary Blair 5 days Daily 1329/ √ √ √ √ √ 1. Please refer to
send at 2°C – 8°C

at ambient temperature. If delay is unavoidable,
MKAK-BPU-U01
RM 70.00
Bacteriology
swab 1330 Specimen
Collection
Procedures for
Disease Division
Laboratory
Testing.
2. Ensure that the

swab shows some
faecal staining to
avoid low quality
of sampling.
3. Delay in
transportation will
of the bacteria.

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
Astrovirus Outbreak EM/ Fresh 5 ml / Sterile 2-8°C √ 1. Sample should
MKAK-BPU-U01
PCR - 5 days
EM - 2 days
PCR-Daily
EM-Daily
PCR-RM214.30
EM - RM 15.00
Virology
Molecular Unit 1321

PCR stool 1.5 cm screw- be collected within
cube capped 5 days after onset
PCR Rectal - Sterile
Swab container 2. A brief concise
with 2.0- history of illness
2.5 ml of and physical
VTM findings is required
especially the date
of onset of illness
and date of sample
collection.
Bacillus cereus
Send within 2 hours after collection at 2°C – 8°C
MKAK-BPU-U01
RM 70.00
Bacteriology
y Collection
Procedures for
Disease Division
Laboratory
Testing.
2. A sample which
is received more
than 2 hours will
be rejected.

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media

MKAK-BPU-U01
RM 70.00
Bacteriology
stool 1330 Specimen
Collection
Procedures for
Disease Division
Laboratory
Testing.
2. Delay in
transportation will
of the bacteria.
2°C – 8°C.
at ambient temperature. If delay is unavoidable, send at
MKAK-BPU-U01
RM 70.00
Bacteriology
swab 1330 Specimen
Collection
Procedures for
Disease Division
Laboratory
Testing.
2. Ensure the swab

shows some faecal
staining to avoid
low quality of
sampling.
3. Delay in
transportation will
of the bacteria.

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
Toxin Bacterial NA slant 7 days Daily 1329/ √ Inoculate pure
Pure isolate
Send at 2°C – 8°C
MKAK-BPU-U01
RM 70.40
Bacteriology
Detectio Culture 1330 single colony into
n to NA slant
Outbreak or Real- 1-3 Sterile 48 Daily 1329/ √ √ √ √ √ 1. Please refer to

Bordetella pertussis
Nasopharyngeal Aspirates (in-patient)
Send immediately after collection at 2°C – 8°C
MKAK-BPU-U01
RM 350.00
Bacteriology
Diagnostic Time PCR ML container hours 1330 Specimen
Collection
Procedures for
Disease Division
Laboratory
Testing.
2. A sample which
is received more
than 2 hours will
be rejected.

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
NA Amies clear 48 Daily 1329/ √ √ √ √ √ 1. Please refer to
Nasopharyngeal swab (contact)
at 2°C – 8°C
Send immediately after collection
MKAK-BPU-U01
RM 350.00
Bacteriology
with hours 1330 Specimen
flocked or Collection
dacron Procedures for
swab Disease Division
Laboratory
Testing.
2. Do not use
Calcium alginate or
cotton swab.
3. Delay in
transportation will
affect the quality
of the sample.
1-3 NA 10 days Daily 1329/ √ √ √ √ 1. Please refer to

Culture & Identification
Nasopharyngeal Aspirates (in-patient)
temperature. DO NOT REFRIGERATE.

Send within 24 hours after collection at ambient
MKAK-BPU-U01
RM 20.00
Bacteriology
ML 1330 Specimen
Collection
Procedures for
Disease Division
Laboratory
Testing.
2. Delay in
transportation will
of the bacteria.
3. A sample which
is received more
than 24 hours will
be rejected.

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
NA Amies 10 days Daily 1329/ √ √ √ √ 1. Please refer to
(contact)
Nasopharyngeal swab
REFRIGERATE.
ambient temperature. DO NOT
Send within 24 hours after collection at
MKAK-BPU-U01
RM 20.00
Bacteriology
Charcoal 1330 Specimen
with Collection
flocked or Procedures for
dacron Disease Division
swab Laboratory
Testing.
2. Do not use
Calcium alginate or
cotton swab.
3. Delay in
transportation will
affect the quality
of the sample.
Outbreak or Culture & Sputum 1-3 Sterile 5 days Daily 1329/ √ √ √ √ 1. Please refer to
Burkholderia cepacia
at 2°C – 8°C.
after collection
Send within 24 hours
MKAK-BPU-U01
RM 70.00
Bacteriology
Diagnostic Sensitivit ML container 1330 Specimen
y Collection
Procedures for
Disease Division
Laboratory
Testing.
2. Delay in
Tracheal 1-3 Sterile 5 days Daily 1329/ √ √ √ √
collection at 2°C – 8°C.
MKAK-BPU-U01
RM 70.00
Bacteriology
transportation will
Aspirate ML container 1330 affect the viability
(in- of the bacteria.
patient)
3. A sample which
is received more
than 24 hours will
be rejected.

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
1-3 Sterile 5 days Daily 1329/ √ √ √ √
(BAL) (in-patient)
Bronchoalveolar lavage

MKAK-BPU-U01
RM 70.00
Bacteriology
ML container 1330
Outbreak or Culture & Sputum 1-3 Sterile 5 days Daily 1329/ √ √ √ √ √ 1. Please refer to
Burkholderia pseudomallei

MKAK-BPU-U01
RM 70.00
Bacteriology
Diagnostic Sensitivit ML container 1330 Specimen
y Collection
Procedures for
Disease Division
Laboratory
Testing.
2. Delay in
transportation will
Tracheal 1-3 Sterile Send within 24 hours after collection 5 days Daily 1329/ √ √ √ √ √
MKAK-BPU-U01
RM 70.00
Bacteriology
at 2°C – 8°C.
of the bacteria.
Aspirate ML container 1330
(in-
patient)

transportation will
Page 18
affect the quality
1. Please refer to
Disease Division
sample shall be
Remark(s)
Procedures for
3. Haemolysed
of the sample.
Laboratory
2. Delay in
Collection
Specimen
rejected.
Testing.
MKA
K. Kinabalu
√
Public Health
Laboratories
MKA
√
Kota Bharu
MKA
√
Johor Bahru
√ √
MKA Ipoh
√
MKAK
Tel No. 03-
1329/
1329/
1330
1330
61261200
Ext. No
Laboratory
Bacteriology Bacteriology
name
Cost/test (RM) RM 70.00 RM 30.00
Frequency of
Daily
Daily
test
5 days
LTAT
5 days
Type of
form
MKAK-BPU-U01 MKAK-BPU-U01
Sample Send within 24 hours after collection at Send immediately after collection
Transportation 2°C – 8°C. at 2°C – 8°C
Container/
Plain Tube
Transport
container
Media
Sterile

3 ML
1-3
ML
Sample Volume
Sample type
Serum
(Preference of sample Bronchoalveolar lavage (BAL) (in-
is in sequence. patient)
Method
Test
Immunofluorescent Antibody Test (IFAT)

Category
Pathogen
transportation will
Page 19
1. Please refer to
Test will done for
investigation and
Disease Division
epidemiological
of the bacteria.
Remark(s)
Procedures for
linkage only.
2. Delay in
Laboratory
Collection
Specimen
outbreak
Testing.
MKA
K. Kinabalu
√
√
Public Health
Laboratories
MKA
√
Kota Bharu
MKA
√
Johor Bahru
√ √
√ √
MKA Ipoh
√
MKAK
Tel No. 03-
1329/
1329/
1329/
1330
1330
1330
61261200
Ext. No
Laboratory
Bacteriology Bacteriology Bacteriology
name
Cost/test (RM) RM 79.30 RM 79.30 RM 350.00
Frequency of Special
Daily
Daily
test Requirement
21 days
21 days
30 days
LTAT
Type of
form
MKAK/BP/ENV/01 Rev 1 MKAK/BP/ENV/01 Rev 1 MKAK-BPU-U01

Sample Send immediately after collection Send immediately after collection
Transportation at ambient temperature at ambient temperature
Container/
Whirl Pack
Whirl Pack
Transport
NA slant
Media
Sterile
Sterile

200 g
200
ML
NA
Sample Volume
Sample type
Isolate
Water
Soil
is in sequence.
Method
MLST
Test
Culture and Identification of B. pseudomallei in environmental sample
Surveillance
Category
Pathogen
Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
Outbreak or Serology Serum 3 ML Plain Tube 5 days Daily 1329/ √ 1. Please refer to
Brucella sp.
at 2°C – 8°C
after collection
Send immediately
MKAK-BPU-U01
RM 30.00
Bacteriology
Diagnostic IgM 1330 Specimen
Collection
Procedures for
Disease Division
Laboratory
Testing.
Serology Serum 3 ML Plain Tube 5 days Daily 1329/ √ 2. Delay in
at 2°C – 8°C
after collection
Send immediately
MKAK-BPU-U01
RM 30.00
Bacteriology
IgG 1330 transportation will
affect the quality
of the sample.
3. Haemolysed
sample shall be
rejected.
Brucella Serum 3 ML Plain Tube 5 days Daily 1329/ √

at 2°C – 8°C
after collection
Send immediately
MKAK-BPU-U01
RM 30.00
Bacteriology
Capt 1330
Real- Isolate NA Culture 2 days Daily 1329/ √ Inoculate pure

at 2°C – 8°C
after collection
Send immediately
MKAK-BPU-U01
RM 350.00
Bacteriology
Time PCR plate 1330 single colony onto
Blood Agar.

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
Outbreak / Fresh Sterile/ 3 days 2002 √ Fresh stool is

Blastocystis
Polymerase chain reaction (PCR)
2 - 3 gram (peanut size)
unavoidable, please send at 2°C – 8°C.

ambient temperature. If delay is
Send immediately after collection at
PER PATH 301
Only working day
RM 70.00
Parasitology
Surveillance stool/ Clean highly
rectal Container/ recommended
swab in Cary Blair
transport transport
media media
Outbreak Culture & Fresh 3-5 Sterile 5 days Daily 1329/ √ √ √ √ 1. Please refer to
Campylobacter jejunii
Send within 2 hours after collection at 2°C – 8°C.
MKAK-BPU-U01
RM 70.00
Bacteriology
y Collection
Procedures for
Disease Division
Laboratory
Testing.
2. A sample which
is received more
than 2 hours will
be rejected.

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
Fresh NA Cary Blair 5 days Daily 1329/ √ √ √ √ 1. Please refer to

MKAK-BPU-U01
RM 70.00
Bacteriology
stool 1330 Specimen
Collection
Procedures for
Disease Division
Laboratory
Testing.
2. A sample which
is received more
than 48 hours will
be rejected.
3. Delay in
transportation will
of the bacteria.
Rectal NA Cary Blair 5 days Daily 1329/ √ √ √ √ 1. Please refer to
send within 48 hours at 2°C – 8°C.

MKAK-BPU-U01
RM 70.00
Bacteriology
swab 1330 Specimen
Collection
Procedures for
Disease Division
Laboratory
Testing.
2. Ensure the swab
shows some faecal
staining to avoid
low quality of
sampling.
3. Delay in
transportation will
of the bacteria.

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
Outbreak RTK Serum 1-3 ml Plain tube 2-8°C √ 1. Sample should

Chikungunya virus
MKAK-BPU-U01
qPCR - 3 days
RTK (IgM & IgG) - 24 hours
qPCR - Daily
RTK (IgM & IgG) - Daily
qPCR - RM277.20
RTK (IgM & IgG) - RM25.00
Virology
Molecular Unit 1321

(Serology with serum be collected within
)/ separator 5 days after onset
qPCR of illness.
2. A brief concise
history of illness
and physical
findings is required
especially the date
of onset of illness
and date of sample
collection.
Outbreak qPCR / Sputum 1-3 ml Sterile 2-8°C √ 1. Sample should
Coronavirus (MERS CoV)
MKAK-BPU-U01
EM - 2 days
qPCR - 3 days
EM - Daily
qPCR- Daily
EM - RM 15.00
qPCR-RM258.20
Virology

Molecular Unit 1321
EM screw be collected within
capped 5 days after onset
-ryngeal screw 2. A brief concise
Endo- 1-3 ml Sterile collection.
tracheal screw
tube capped
aspirate container
Broncho- 1-3 ml Sterile
alveolar screw
lavage capped
container

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
Throat - Sterile
swab container
with 2.0-
2.5 ml of
VTM
Nasal - Sterile
Pharynge container
al Swab with 2.0-
2.5 ml of
VTM
Nasal - Sterile
Swab container
with 2.0-
2.5 ml of
VTM
Tissue 1.5cm Sterile
biopsy / cube screw
autopsy in a capped
few container
drops
of
VTM

transportation will
Page 25
1. Please refer to
Disease Division
of the bacteria.
Remark(s)
Procedures for
2. Delay in
Laboratory
Collection
Specimen
Testing.
MKA
K. Kinabalu
√
√
Public Health
Laboratories
MKA
√
Kota Bharu
MKA
√
Johor Bahru
√ √
√ √
MKA Ipoh
MKAK
Tel No. 03-
1329/
1329/
1330
1330
61261200
Ext. No
Laboratory
name
Frequency of
Daily
Daily
test
5 days
5 days
LTAT
Type of
form
Sample Send within 24 hours after collection Send within 24 hours after collection
Transportation at ambient temperature. If delay is at ambient temperature. If delay is
unavoidable , send at 2°C – 8°C unavoidable , send at 2°C – 8°C
Amies clear
Amies clear
Container/
Transport
Media

NA
NA
Sample Volume
(contact)
Sample type
Throat
swab
is in sequence.
Culture &
Sensitivit
Method
Test
y
Outbreak or
Diagnostic
Category
Pathogen
Corynebacterium diphtheriae
single colony onto
single colony onto
Page 26
Remark(s)
Inoculate pure
Inoculate pure
Blood Agar.
Blood Agar.
MKA
K. Kinabalu
√
√
Public Health
Laboratories
MKA
√
Kota Bharu
MKA
√
Johor Bahru
√ √
√ √
√ √
MKA Ipoh
√
MKAK
Tel No. 03-
1329/
1329/
1329/
1329/
1330
1330
1330
1330
61261200
Ext. No
Laboratory
Bacteriology Bacteriology Bacteriology Bacteriology
name
Cost/test (RM) RM 70.00 RM 70.00 RM 350.00 RM 100.00
Frequency of
Daily
Daily
Daily
Daily
test
5 days
5 days
2 days
7 days
LTAT
Type of
form
MKAK-BPU-U01 MKAK-BPU-U01 MKAK-BPU-U01 MKAK-BPU-U01

Send at
Send at
Sample Send immediately after Send immediately after 2°C –
2°C –
Transportation collection at 2°C – 8°C collection at 2°C – 8°C
8°C
8°C
Blood Agar
Blood Agar
Container/
Transport
container
container
Media
Sterile
Sterile

Sample Volume Pure isolate Pure isolate
1-3
ML
NA
Sample type
Bacterial
Bacterial
Culture
Culture
(Preference of sample Nasopharyngeal Aspirate Pseudomembrane
is in sequence. (in-patient) exudates (in-patient)
Toxigeni-
Method
Detectio
ELEK for
PCR for
Testing
Test
Toxin
city
n
Category
Pathogen
Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
Multilocu Bacterial Blood Agar 30 days 1329/ √ Test will done for
Pure isolate
MKAK-BPU-U01
Requirement
Special
RM 350.00
Bacteriology
s Culture 1330 outbreak
Sequenc investigation and
e Typing epidemiological
linkage only.
Outbreak or Serology Serum 3 ML Plain Tube 5 days Daily 1329/ √ 1. Please refer to
Coxiella burnettii
at 2°C – 8°C
MKAK-BPU-U01
RM 30.00
Bacteriology
Collection
Procedures for
Disease Division
Laboratory
Testing.
2. Delay in
transportation will
affect the quality
of the sample.
Serology Serum 3 ML Plain Tube 5 days Daily 1329/ √ 3. Haemolysed

at 2°C – 8°C
MKAK-BPU-U01
RM 30.00
Bacteriology
IgG 1330 sample shall be
rejected.

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
Outbreak qPCR Mouth - Sterile 2-8°C √ 1. Sample should

Coxsackie A16
Borang Permohonan Ujian Makmal HFMD
qPCR - 3 days
qPCR- Daily
qPCR-RM292.00
Virology
Molecular Unit 1321

Ulcer container be collected within
Swab with 2.0- 5 days after onset
2.5 ml VTM of illness.
2. A brief concise
Vesicle - Sterile
history of illness
Swab container
and physical
with 2.0-
2.5 ml VTM
especially the date
Stool 5g or Sterile of onset of illness
'pea Screw and date of sample
size' capped collection.
container
Rectal - Sterile
Swab container
with 2.0-
2.5 ml VTM
Throat - Sterile
Swab container
with 2.0-
2.5 ml VTM
Pleural 1-3 ml Sterile
fluid screw
capped
container
Cerebro- 1-3 ml Sterile
spinal Screw
fluid capped
(CSF) container
Serum 1-3 ml Plain tube
with serum
separator

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media

biopsy / cube screw
autopsy in a capped
(post- few container
mortem drops
case) of
VTM
Outbreak/ PCR Fresh Sterile/ 3 days 2002 √ Fresh stool is
Cryptosporidium

PER PATH 301
Only working day
RM 70.00
Parasitology
swab in Cary Blair
transport transport
media media
Dengue Outbreak/ qPCR Serum 1-3 ml Plain tube 2-8°C √ √ √ √ √ Refer to :
MKAK-BPU-D02-rev_Nov_2015
qPCR - 3 days
qPCR- Daily
qPCR-RM277.20
Virology
Molecular Unit 1321

Surveillance with serum 1. Pekeliling KPK
separator Malaysia Bil
14/2011: Program
DVSS;
Bil(23)dlmKKM-
CSF 1 ml Sterile
171/BKP/07/35/05
screw
19Jld4, 25 Januari
capped
2012
container
2.Survelan
Serotaip Virus
Denggi bagi Kes

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
Tissue 1.5cm Sterile "Severe Dengue"

biopsy / cube screw di ICU;
autopsy in a capped Bil(2)dlmKKM-
(post- few container 171/BKP/09/41/06
mortem drops 01Jld.12, 13
case) of Disember 2013
VTM
Outbreak/ PCR Fresh Sterile/ 2002 √ Fresh stool is
Dientamoeba

PER PATH 301
3 days
Only working day
RM 70.00
Parasitology
swab in Cary Blair
transport transport
media media

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
Ebolavirus Outbreak EM/ Serum 1-3 Plain tube 2-8°C √ 1. Sample should
MKAK-BPU-U01
qPCR - 3 days
EM - 2 days
qPCR-Daily
EM-Daily
qPCR-RM257.00
EM - RM 15.00
Virology
Molecular Unit 1321

qPCR ml with serum be collected within
separator 5 days after onset
of illness.
2. A brief concise
history of illness
and physical
especially the date
of onset of illness
and date of sample
collection.
3. Inform MKAK
prior to samples
collection
Outbreak/ PCR Fresh Sterile/ 2002 √ Fresh stool is
Entamoeba histolytica
transported in a cold chain (4°C)

Biohazard Zip Lock plastic bag and
PER PATH 301
3 days
Only working day
Parasitology
RM 70.00
Surveillance stool Clean highly
Container recommended

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
Enteric bacterial Pathogens
at 2°C – 8°C
MKAK-BPU-U01
RM 70.00
Bacteriology
Sensitivit stool gm Container 1330 Specimen
y Collection
Procedures for
Disease Division
Laboratory
Testing.
2 .A sample which
is received more
than 2 hours will
be rejected.
at 2°C – 8°C
at ambient temperature. If delay is unavoidable, send
MKAK-BPU-U01
RM 70.00
Bacteriology
stool 1330 Specimen
Collection
Procedures for
Disease Division
Laboratory
Testing.
2. Delay in
transportation will
of the bacteria.

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media

MKAK-BPU-U01
RM 70.00
Bacteriology
swab 1330 Specimen
Collection
Procedures for
Disease Division
Laboratory
Testing.
2. Ensure the swab

shows some faecal
staining to avoid
low quality of
sampling.
3. Delay in
transportation will
of the bacteria.
Sporadic / VI Mouth - Sterile 2-8°C √ 1. Refer to Surat
Enterovirus (HFMD)
HFMD
Borang Permohonan Ujian Makmal
VI - 21 days
VI - Daily
Virus Isolation - RM100.00
Virology

Surveillance Ulser container Arahan
Swab with 2.0- Pengukuhan
2.5 ml VTM Survelan Makmal
untuk
Vesicle - Sterile PenyakitTangan,
Swab container kaki dan mulut
with 2.0- (HFMD;
2.5 ml VTM (7)dlmKKM-
Stool 5g or Sterile 171/BKP/02/10/01
'pea Screw 42Jld3, Ogos 2012
size' capped 2. Enterovirus
container Screenig Panel
(Enterovirus 71,

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
Rectal - Sterile Coxsackie A16 &

Swab container A24, Coxsackie B,
with 2.0- Enterovirus 70,
2.5 ml VTM Echovirus type
4,6,9,11 &30 and
Throat - Sterile Poliovirus type 1,2
Swab container & 3)
with 2.0-
2.5 ml VTM

fluid screw
capped
container

spinal Screw
fluid capped
(CSF) container
with serum
separator
biopsy/a cube Screw
utopsy in a capped
(post few container
mortem) drops
of
VTM

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
Outbreak qPCR Mouth - Sterile 2-8°C √ 1. Sample should

Enterovirus 71
Borang Permohonan Ujian Makmal HFMD
qPCR - 3 days
qPCR- Daily
qPCR-RM292.00
Virology
Molecular Unit 1321

Ulser container be collected within
Swab with 2.0- 5 days after onset
2.5 ml VTM of illness.
Vesicle - Sterile 2. A brief concise

Swab container history of illness
with 2.0- and physical
2.5 ml VTM findings is required
especially the date
Stool 5g or Sterile
of onset of illness
'pea Screw
and date of sample
size' capped
collection.
container
Rectal - Sterile
Swab container
with 2.0-
2.5 ml VTM
Throat - Sterile
Swab container
with 2.0-
2.5 ml VTM

fluid screw
capped
container
Cerebros 1-3 ml Sterile

pinal Screw
fluid capped
(CSF) container

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media

with serum
separator
biopsy / cube screw
autopsy in a capped
(post- few container
mortem drops
case) of
VTM
Escherichia coli (Diarrheagenic)
Send within 2 hours after collection at 2°C – 8°C
MKAK-BPU-U01
RM 70.00
Bacteriology
Sensitivit stool gm Container 1330 Specimen
y Collection
Procedures for
Disease Division
Laboratory
Testing.
2 .A sample which
is received more
than 2 hours will
be rejected.

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media

MKAK-BPU-U01
RM 70.00
Bacteriology
stool 1330 Specimen
Collection
Procedures for
Disease Division
Laboratory
Testing.
2. Delay in
transportation will
of the bacteria.
2°C – 8°C.
at ambient temperature. If delay is unavoidable, send at
MKAK-BPU-U01
RM 70.00
Bacteriology
swab 1330 Specimen
Collection
Procedures for
Disease Division
Laboratory
Testing.
2. Ensure the swab

shows some faecal
staining to avoid
low quality of
sampling.
3. Delay in
transportation will
of the bacteria.

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
PCR Bacterial NA slant Send at 3 days Daily 1329/ √ √ Inoculate pure
Pure isolate
MKAK-BPU-U01
RM 350.00
Bacteriology
Culture 2°C – 1330 single colony onto
8°C Nutrient Agar.
Full Bacterial NA slant Send at 30 days Daily 1329/ √ Inoculate pure
Pure isolate
MKAK-BPU-U01
RM 20.00
Bacteriology
Serotypin Culture 2°C – 1330 single colony onto
g 8°C Nutrient Agar.
Filaria Outbreak/ Slide Slide 24 2002 √ √ √ √ √ NA

Microscopy Examination
Peripheral blood smear
60 ul Thick blood smear
PER PATH 301
Only working day
RM 10.00
Parasitology
Diagnostic Folder/ Box hours
Slide Box

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
Outbreak/ qPCR Serum 1-3 ml Plain tube 2-8°C √ √ Refer to:

Flavivirus (Pan Flavivirus)
qPCR - 3 days
qPCR- Daily
qPCR-RM214.30
Virology
Molecular Unit 1321

Surveillance with serum 1. Surat Arahan
separator Pelaksanaan
Program Survelan
Makmal Flavivirus;
MKAK 600-
1/7/5(3), 3
Disember 2015.
2. Surat Arahan
Penambahan
CSF 1 ml Sterile
Penghantaran
screw
Sampel Survelan
capped
Makmal Flavivirus;
container
MKAK 600-
1/7/5(10), 24 Jun
2016.
Giardia Outbreak/ PCR Fresh Sterile/ 3 days 2002 √
in a cold chain (4°C)

Biohazard Zip Lock plastic bag and transported
PER PATH 301
Only working day
Parasitology
RM 70.00
Surveillance stool Clean
Container

transportation will
Page 40
1. Please refer to
Disease Division
of the bacteria.
Remark(s)
Procedures for
2. Delay in
Laboratory
Collection
Specimen
Testing.
MKA
K. Kinabalu
Public Health
Laboratories
MKA
√
Kota Bharu
MKA
√
Johor Bahru
√ √
√ √
MKA Ipoh
MKAK
Tel No. 03-
1329/
1329/
1330
1330
61261200
Ext. No
Laboratory
name
Frequency of
Daily
Daily
test
5 days
5 days
LTAT
Type of
form
Sample Send within 24 hours after collection at ambient temperature. If Send within 24 hours after
Transportation delay is unavoidable, send at 2°C – 8°C collection at 2°C – 8°C.
Container/
Transport
container
Media
Sterile
Charcoal
Amies

1-3
ML
Sample Volume
NA
Sample type
(Preference of sample Nasopharyngeal Aspirates Iin-
is in sequence. patient)
Culture &
Sensitivit
Method
Test
y
Outbreak or
Diagnostic
Category
Pathogen
Haemophilus influenzae
Page 41
Remark(s)
MKA
K. Kinabalu
Public Health
Laboratories
MKA
√
Kota Bharu
MKA
√
Johor Bahru
√ √
√ √
√ √
MKA Ipoh
MKAK
Tel No. 03-
1329/
1329/
1329/
1330
1330
1330
61261200
Ext. No
Laboratory
name
Frequency of
Daily
Daily
Daily
test
5 days
5 days
5 days
LTAT
Type of
form
MKAK-BPU-U01 MKAK-BPU-U01 MKAK-BPU-U01

Sample Send within 24 hours after Send within 24 hours after Send within 24 hours after collection
Transportation collection at 2°C – 8°C. collection at 2°C – 8°C at 2°C – 8°C
Container/
Transport
container
container
container
Media
Sterile
Sterile
Sterile

1-3
1-3
1-3
ML
ML
ML
Sample Volume
Sample type
Sputum
(Preference of sample Tracheal Aspirate (in- Bronchoalveolar lavage (BAL) (in-

is in sequence. patient) patient)
Method
Test
Category
Pathogen
Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
CSF 1 ML Sterile 5 days Daily 1329/ √ √ √ √ CSF which is
at 2°C – 8°C
Send within 1 hour after collection
MKAK-BPU-U01
RM 70.00
Bacteriology
container 1330 received more
than 1 hour is not
sutable for
culturing.
PCR CSF 1 ML Sterile 2 days Daily 1329/ √ 1. Please refer to

Send immediately after
MKAK-BPU-U01
RM 350.00
Bacteriology
container 1330 Specimen
Collection
Procedures for
Disease Division
Laboratory
Testing.
2. Delay in
transportation will
Plasma 3 ML EDTA Tube 2 days Daily 1329/ √
MKAK-BPU-U01
RM 350.00
Bacteriology
of the bacteria.
1330

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
Serum 3 ML Plain Tube 2 days Daily 1329/ √

MKAK-BPU-U01
RM 350.00
Bacteriology
1330
Surveillance Serotypin Bacterial Chocolate Send 7 days Daily 1329/ √ Inoculate pure
Pure isolate
MKAK-BPU-U01
RM 20.00
Bacteriology
g Culture Agar at 2°C – 1330 single colony onto
8°C Chocholate Agar.
Surveillance Multilocu Bacterial Chocolate Send 30 days 1329/ √ Test will done for
Pure isolate
MKAK-BPU-U01
Requirement
Special
RM 350.00
Bacteriology
s Culture Agar at 2°C – 1330 outbreak
Sequenc 8°C investigation and
e Typing epidemiological
linkage only.
Outbreak EM/ Fresh 5 ml / Sterile 2-8°C √ A brief concise

Hepatitis A virus
MKAK-BPU-U01
PCR - 5 days
EM - 2 days
Serology- Daily
PCR-Daily
EM-Daily
Serology-RM70.00
PCR-RM214.30
EM - RM 15.00
Virology
Serology Unit 4027

Molecular Unit1321
EM Unit 1320 / 1335
PCR stool 1.5 cm screw- history of illness
cube capped and physical
container findings is required
especially the date
of onset of illness
and date of sample
collection.

Page 44
2. Test requested
by appointment
1. Screening for
MOH staff only
Remark(s)
MKA
K. Kinabalu
Public Health
Laboratories
MKA
√
Kota Bharu
MKA
√
Johor Bahru
√ √
MKA Ipoh
MKAK
Tel No. 03-
61261200 Serology Unit 4027
Ext. No
Laboratory
Virology
name
HBsAg = RM34.00
Cost/test (RM)
antiHBs = RM33.40
Frequency of
By appointment
test
7 days
LTAT
Type of
form
MKAK-BPU-U01
Sample
2-8°C
Transportation
Container/
Transport
container
with 2.0-
2.5 ml of
Media
Sterile
Plain tube/gel tube / EDTA tube / Plain tube / EDTA tube / Lithium tube /
VTM
Lithium tube / Sodium heparinized tube Sodium heparinized tube / citrated tube

1-3 ml
1-3
ml
Sample Volume
-
Sample type
Serum /
Serum /
Plasma
Plasma
Rectal
Swab
is in sequence.
Serology
Serology
antiHBs)
Method
(HBsAg
Test
PCR
and
Diagnostic
Category
Pathogen
Hepatitis B
Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
Outbreak IFAT/ Sputum 1-3 Sterile 2-8°C √ 1. Sample should

Herpes viruses (Herpes simplex type 1, Cytomegalovirus, Varicella Zoster virus)
MKAK-BPU-U01
VI - 21 days
IFAT - 7 days
VI - Daily
IFAT - Daily
VI -100.00
IFAT - RM 68.00
Virology
VI Unit 1315 / 1325

VI ml Screw be collected within
Nasopha 1-3 Sterile 2. A brief concise

-rangeal ml Screw history of illness
Aspirate capped and physical
especially the date
Tracheal 1-3 Sterile of onset of illness
/ Endo- ml Screw and date of sample
tracheal capped collection.
tube container
Aspirate
Broncho- 1-3 Sterile
alveolar ml Screw
lavage capped
container
Throat - Sterile
swab container
with 2.0-
2.5 ml of
VTM
Nasal - Sterile
Pharan- container
geal with 2.0-
Swab 2.5 ml of
VTM

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
Nasal - Sterile
Swab container
with 2.0-
2.5 ml of
VTM
Lungs 1.5cm Sterile
tissue cube Screw
biopsy / in a capped
autopsy few container
drops
of
VTM
Outbreak PCR Mouth - Sterile 2-8°C √ 1. Sample should
Herpes Simplex viruses
MKAK-BPU-U01
PCR-5 days
PCR-Daily
PCR-RM210.20
Virology
Molecular Unit1321
ulcer container be collected within
swab with 2.0- 5 days after onset
2.5 ml of of illness.
VTM
2. A brief concise
Skin - Sterile history of illness
lesion container and physical
swab with 2.0- findings is required
2.5 ml of especially the date
VTM of onset of illness
and date of sample
Broncho- 1-3 ml Sterile collection.
alveolar screw
lavage capped
container
Naso 1-3 ml Sterile

Pharyn- screw
geal capped
aspirate container

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
Tracheal 1-3 ml Sterile

aspirate screw
capped
container
Nasopha - Sterile
-ryngeal container
swab with 2.0-
2.5 ml of
VTM
Rectal - Sterile
swab container
with 2.0-
2.5 ml of
VTM
Eye swab - Sterile

container
with 2.0-
2.5 ml of
VTM
Genital - Sterile
swab container
with 2.0-
2.5 ml of
VTM

spinal Screw
fluid capped
(CSF) container

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
HIV Diagnostic Serology Serum / 1-3 Plain tube / 2-8°C √ √ √ √ Test requested by
(HIV - AIDS-97)
Borang Permohonan Ujian HIV
Serology-7 days
By appointment
Particle Agglutination: RM 53.40

EIA: RM=50.40
Virology
Serology Unit 4027

Plasma ml potassium appointment
EDTA tube
Outbreak IFAT/ Sputum 1-3 ml Sterile 2-8°C √ √ √ √ √ 1. Sample should

Influenza A (H1, H3, H1N1)
MKAK-BPU-U01
qPCR-3 days
EM - 2 days
IFAT - 7 days
qPCR-Daily
EM - Daily
IFAT - Daily
qPCR-RM 292.00
EM - RM 15.00
IFAT - RM 35.00
Virology
Molecular Unit 1321

EM Unit 1320 / 1335
VI Unit 1315 / 1325
EM/ screw be collected within
qPCR capped 5 days after onset
Nasopha 1-3 ml Sterile 2. A brief concise

-rangeal screw history of illness
Tracheal 1-3 ml Sterile especially the date
/ screw of onset of illness
Endotra- capped and date of sample
cheal container collection.
tube
Aspirate
alveolar screw
lavage capped
container

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
qPCR / Throat - Sterile

EM swab container
with 2.0-
2.5 ml of
VTM
Nasal - Sterile
Pharyn- container
geal with 2.0-
Swab 2.5 ml of
VTM
Nasal - Sterile
Swab container
with 2.0-
2.5 ml of
VTM
Lungs 1.5cm Sterile

tissue cube screw
in a capped
few container
drops
of
VTM

Influenza A (H5N1)
MKAK-BPU-U01
qPCR-3 days
qPCR-Daily
qPCR-RM258.20
Virology
Molecular Unit 1321

2. A brief concise
history of illness
and physical

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
Nasopha 1-3 ml Sterile findings is required

-rangeal screw especially the date
Aspirate capped of onset of illness
collection.
/ screw
Endotra- capped
cheal container
tube
Aspirate
alveolar screw
lavage capped
container

EM swab container
with 2.0-
2.5 ml of
VTM
Nasal - Sterile
Pharynge container
al Swab with 2.0-
2.5 ml of
VTM
Nasal - Sterile
Swab container
with 2.0-
2.5 ml of
VTM

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
Lungs 1.5cm Sterile

tissue cube screw
in a capped
few container
drops
of
VTM

Influenza A (H7N9)
MKAK-BPU-U01
qPCR-3 days
qPCR-Daily
qPCR-RM258.20
Virology
Molecular Unit 1321


-rangeal screw history of illness
especially the date
of onset of illness
Tracheal 1-3 ml Sterile and date of sample
/ screw collection.
Endotra- capped
cheal container
tube
Aspirate
alveolar screw
lavage capped
container

EM swab container
with 2.0-

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
2.5 ml of
VTM
Nasal - Sterile
Pharynge container
al Swab with 2.0-
2.5 ml of
VTM
Nasal - Sterile
Swab container
with 2.0-
2.5 ml of
VTM
Lungs 1.5cm Sterile

tissue cube screw
in a capped
few container
drops
of
VTM
Outbreak IFAT/ Sputum 1-3 ml Sterile 2-8°C √ √ √ √ √ 1. Sample should

Influenza B
MKAK-BPU-U01
qPCR-3 days
EM - 2 days
IFAT - 7 days
qPCR-Daily
EM - Daily
IFAT-Daily
qPCR-RM258.20
EM - RM 15.00
IFAT-RM 35.00
Virology
Molecular Unit1321
EM Unit 1320 / 1335
VI Unit 1315 / 1325

-ryngeal screw history of illness

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
Tracheal 1-3 ml Sterile especially the date

/ screw of onset of illness
Endotra- capped and date of sample
cheal container collection.
tube
Aspirate
Broncho 1-3 ml Sterile
alveolar screw
lavage capped
container

EM swab container
with 2.0-
2.5 ml of
VTM
Nasal - Sterile
Pharyn- container
geal with 2.0-
Swab 2.5 ml of
VTM
Nasal - Sterile
Swab container
with 2.0-
2.5 ml of
VTM
Lungs 1.5cm Sterile
tissue cube screw
in a capped
few container
drops
of
VTM

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
ILI VI Throat - Sterile 2-8°C √ Refer to Malaysia

Influenza A / B viruses
Malaysia Influenza -ILI surveillance form (annex 6)
VI - 21 days
VI - Daily
VI - RM35.00
Virology
VI Unit 1315 / 1325

Surveillance swab container Influenza
with 2.0- Surveillance
2.5 ml of Protocol (MISP),
VTM Nov 2015.
1. Sample should
be collected within
5 days after onset
of illness.
2. A brief concise
history of illness
and physical
especially the date
of onset of illness
and date of sample
collection.
Outbreak/ Serology/ CSF 1-3 ml Plain 2-8°C √ √ Refer to Surat
Japanese encephalitis virus
qPCR-3 days
Serology-7 days
qPCR-Daily
Serology-Daily
qPCR-RM257.00
Serology -RM 181.00
Virology
Molecular Unit1321
Serology Unit 4027
Surveillance qPCR tube/gel Pekeliling KPK
tube Malaysia bil
(without 6/2012: Survelan
anticoa- JE Kebangsaan;
gulant) Mkaka 600-
1/1/1(13), 3
Februari 2012
1.Sample should
be collected within
5 days after onset
of illness.

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
Serum 1-3 ml Sterile

screw 2. A brief concise
capped history of illness
especially the date
of onset of illness
and date of sample
collection.
Outbreak Sputum 1-3 ml Sterile 5 days Daily 1329/ √ √ √ √ √ 1. Please refer to
Klebsiella pneumoniae
Culture & Sensitivity
at 2°C – 8°C
MKAK-BPU-U01
RM 70.00
Bacteriology
container 1330 Specimen
Collection
Procedures for
Disease Division
Laboratory
Testing.
2. Delay in
transportation will
of the bacteria.
Tracheal 1-3 ml Sterile 5 days Daily 1329/ √ √ √ √ √

at 2°C – 8°C
MKAK-BPU-U01
RM 70.00
Bacteriology
Aspirate container 1330
(in-
patient)

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
Broncho- 1-3 ml Sterile 5 days Daily 1329/ √ √ √ √ √
at 2°C – 8°C
MKAK-BPU-U01
RM 70.00
Bacteriology
alveolar container 1330
lavage
(BAL) (in-
patient)
Outbreak or Sputum 1-3 ml Sterile 14 days Daily 1329/ √ 1. Please refer to

Legionella pneumophilia
Culture & Identification
at 2°C – 8°C
after collection
Send immediately
MKAK-BPU-U01
RM 70.00
Bacteriology
Diagnostic container 1330 Specimen
Collection
Procedures for
Disease Division
Laboratory
Testing.
Tracheal 1-3 ml Sterile 14 days Daily 1329/ √
at 2°C – 8°C
after collection
Send immediately
MKAK-BPU-U01
RM 70.00
Bacteriology
Aspirate container 1330 2. Delay in
(in- transportation will
patient) affect the viability
of the bacteria.
Broncho- 1-3 ml Sterile 14 days Daily 1329/ √

at 2°C – 8°C
after collection
Send immediately
MKAK-BPU-U01
RM 70.00
Bacteriology
alveolar container 1330
lavage
(BAL) (in-
patient)

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
Outbreak or Water 1 Insulated 21 days 1329/ √ √ √ √ √ 1. Please refer to
Identification
Culture , Enumeration and

MKAK/BP/ENV/01 Rev 1
By Appointment
RM 200.64
Bacteriology
Diagnostic from LITER Sterile 1330 Specimen
Surveillance Cooling screw cap Collection
tower container Procedures for
Disease Division
Laboratory
Testing.
2. Delay in
transportation will
of the bacteria.
Outbreak or Serology Serum 3 ML Plain Tube 3 days Daily 1329/ √ √ √ √ √ 1. Please refer to
Leptospira sp.
at 2°C – 8°C
MKAK-BPU-U01
RM 30.00
Bacteriology
Surveillance Collection
Procedures for
Disease Division
Laboratory
Testing.
2. Delay in
transportation will
affect the quality
of the sample.
3. Haemolysed
sample shall be
rejected.

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
Serum 3 ML Plain Tube 7 days Daily 1329/ √ √ √ √ √ Requester shall
Test (MAT)
Microagglutination
Serology
at 2°C – 8°C
after collection
Send immediately
MKAK-BPU-U01
RM 20.00
Bacteriology
1330 attach the
Serology IgM
Leptospira result
Real- Plasma 3 ML EDTA Tube 2 days Daily 1329/ √ √ 1. Please refer to

MKAK-BPU-U01
RM 350.00
Bacteriology
Time PCR 1330 Specimen
Collection
Procedures for
Disease Division
Laboratory
Testing.
2. Delay in
transportation will
of the bacteria.
MLST Isolate NA NA Send at 30 days 1329/ √ Test will done for
MKAK-BPU-U01
Special Requirement
RM 350.00
Bacteriology
2°C – 1330 outbreak
8°C investigation and
epidemiological
linkage only.

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
Water 250 Sterile 21 days Daily 1329/ √ √ √ √ √ 1. Please refer to
sample
Culture and detection of Leptospira sp. in environmental
temperature.
collection at ambient
RM 350.00
Bacteriology
ML Whirl Pack 1330 Specimen
Collection
Procedures for
Disease Division
Laboratory
Testing.
2. Delay in
transportation will
Soil 200 g Sterile 21 days Daily 1329/ √ √ √ √ √ affect the viability
temperature.
collection at ambient
RM 350.00
Bacteriology
Whirl Pack 1330 of the bacteria.
Outbreak Fresh 3-5 Sterile 5 days Daily 1329/ √ √ √ √ √ 1. Please refer to

Listeria monocytogenes
at 2°C – 8°C
MKAK-BPU-U01
RM 70.00
Bacteriology
stool gm container 1330 Specimen
Collection
Procedures for
Disease Division
Laboratory
Testing.
2 .A sample which
is received more
than 2 hours will
be rejected.

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media

MKAK-BPU-U01
RM 70.00
Bacteriology
stool 1330 Specimen
Collection
Procedures for
Disease Division
Laboratory
Testing.
2. Delay in
transportation will
of the bacteria.
send at 2°C – 8°C.

MKAK-BPU-U01
RM 70.00
Bacteriology
swab 1330 Specimen
Collection
Procedures for
Disease Division
Laboratory
Testing.
2. Ensure the swab

shows some faecal
staining to avoid
low quality of
sampling.
3. Delay in
transportation will
of the bacteria.

be collected within
5 days after onset
Page 61
2. A brief concise
1. Sample should
history of illness
Remark(s)
Management
Guideline of
Reference:
Malaria in
of illness.
Malaysia
MKA
K. Kinabalu
√
√
Public Health
Laboratories
MKA
√
Kota Bharu
MKA
√
Johor Bahru
√ √
√ √
√ √
√ √
MKA Ipoh
√
MKAK
Tel No. 03- Virus Isolation Unit
2002
2002
2002
2002
61261200
1315/1325
Ext. No
Laboratory Parasitolog
Parasitology Parasitology Parasitology Virology
name y
IFAT - RM 35.00
VI - RM 75.00
Frequency of IFAT - Daily
Daily
Daily
Daily
Daily
test VI - Daily
3 days
3 days
3 days
hours
IFAT - 7 days
LTAT
24
VI - 21 days
Type of
PER PATH
form
PER PATH 301 PER PATH 301 PER PATH 301 MKAK-BPU-U01
301
Slide Box Biohazard Slide Box Biohazard Zip Lock
2-8°C
Sample
Transportation Zip Lock Sterile plastic bag
plastic bag
and Filter paper
Blood Tube
Container/
(Whatman
with EDTA
Transport
Grade 1)
transporte
capped
capped
Media
Sterile
Sterile
Folder
Folder
screw
screw
Slide
Slide
d in a cold

chain (4°C)
or minus
20oC if a
1-3 ml
1-3 ml
blood
3 to 5
Slide with thin Slide with thin Approx. 125 ul of blood

ml of
Sample Volume longer

and thick smear and thick smear spotted on filter paper
period is
needed for
Nasopha
Sample type
Aspirate
-rangeal
Blood in
Sputum
spot on
Whole
BFMP
BFMP
paper
Blood
SLIDE
SLIDE
EDTA
filter
(Preference of sample transporta
is in sequence.
tion
Isolation
Method
IFAT/
Microscopy
Virus
Test
(VI)
Examination
PCR
Outbreak
Category
For confirmation Outbreak/ Confirmation

Pathogen
Metapneumovirus
Malaria
(Human
Metapneumovirus)
Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
Throat - Sterile and physical

swab container findings is required
with 2.0- especially the date
2.5 ml of of onset of illness
VTM and date of sample
collection.
Nasal - Sterile
Pharan- container
geal with 2.0-
Swab 2.5 ml of
VTM
Nasal - Sterile
Swab container
with 2.0-
2.5 ml of
VTM
/ endo- screw
tracheal capped
tube
Aspirate

alveolar screw
lavage capped
Lungs 1.5cm Sterile

tissue cube screw
autopsy few
drops
of
VTM

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
Outbreak/ Serology Serum 1-3 ml Plain tube 2-8°C √ Refer to Measles

Measles virus
MSLF:01/2004
Measles: Borang Permohonan dan Keputusan Ujian Makmal
VI - 21 days
qPCR - 3 days
Serology-4 days
VI - Daily
qPCR- Daily
Serology - Daily
VI - RM 75.00
qPCR- RM257.00
Serology - RM 61.34
Virology
Virus Isolation Unit 1315/1325

Molecular Unit 1321
Serology Unit 4027
√ (Serology only)
Surveillance with serum Surveillance
separator Manual, 1st
Edition,
qPCR/ Throat - Sterile September 2004,
Virus swab container Specimens
Isolation with 2.0- Collection for
(VI) 2.5 ml of Laboratory
VTM Investigation
Urine 10 ml Sterile 1.Blood/serum

of screw should be taken
urine capped any time up to 28
(Early container days of rash onset.
morni
ng 2. Respiratory
first secretion should
void) be taken 1 – 5 days
of rash onset.
-ryngeal screw 3. Respiratory
secretion capped secretion
container (nasopharyngeal
specimen) should
Tracheal 1-3 ml Sterile be taken 1 – 7 days
aspirate screw of rash onset.
capped
container

Page 64
Remark(s)
MKA
K. Kinabalu
Public Health
Laboratories
MKA
√
Kota Bharu
MKA
√
Johor Bahru
√ √
√ √
MKA Ipoh
MKAK
Tel No. 03-
1329/
1329/
1330
1330
61261200
Ext. No
Laboratory
name
Cost/test (RM) RM70.00 RM70.00
Frequency of
Daily
Daily
test
5 days
5 days
LTAT
Type of
form
Transportation at ambient temperature. If delay is at 2°C – 8°C.
Container/
Transport
Charcoal
Charcoal
Media
Amies
Amies

1-3
ML
NA
Sample Volume
Sample type
Nasopharyngeal swab (contact) Nasopharyngeal Aspirates (in-patient)
is in sequence.
Method
Test

Outbreak
Category
Pathogen
Moraxella catarrhalis
Page 65
Remark(s)
MKA
K. Kinabalu
Public Health
Laboratories
MKA
√
Kota Bharu
MKA
√
Johor Bahru
√ √
√ √
√ √
MKA Ipoh
MKAK
Tel No. 03-
1329/
1329/
1329/
1330
1330
1330
61261200
Ext. No
Laboratory
name
Cost/test (RM) RM70.00 RM70.00 RM70.00
Frequency of
Daily
Daily
Daily
test
5 days
5 days
5 days
LTAT
Type of
form

Sample Send within 24 hours after Send within 24 hours after Send within 24 hours after
Transportation collection at 2°C – 8°C collection at 2°C – 8°C collection at 2°C – 8°C
Container/
Transport
container
container
container
Media
Sterile
Sterile
Sterile

1-3ML
1-3ML
1-3ML
Sample Volume
Sample type
Tracheal
Aspirate
patient)
Sputum
Bronchoalveolar lavage (BAL)
(in-
or
is in sequence. (in-patient)
Method
Test
Category
Pathogen
and date of sample
the highest yield of

be collected within
especially the date
Please consult the

recommended for
Science Officer in-

5 days after onset
Page 66
of onset of illness
2. A brief concise
1. Sample should
AFB Microscopy.
history of illness
specimens have
i. Early morning
Remark(s)
and physical
collection.
of illness.
It is not
charge.
AFB.
MKA
K. Kinabalu
√
√
Public Health
Laboratories
MKA
√
Kota Bharu
MKA
√
Johor Bahru
√ √
√ √
MKA Ipoh
√
MKAK
Tel No. 03-
1327/
1327/
Molecular Unit 1321

1328
1328
61261200
VI Unit 1315/1325
Ext. No
Laboratory
TB
TB
Virology
name
VI - RM 100.00
qPCR-RM257.00
Frequency of VI - Daily
Daily
Daily
test qPCR- Daily

VI - 21 days
LTAT
1 (working day) 1 (working day)

qPCR - 3 days
Type of
form
MKAK-BPU-U01
TBIS
TBIS
20C
20C
4°C – 8°C. Specimens should be
2-8°C
Sample
Transportation kept cool during transportation
but not be frozen.
-
Container/
Clean leak
Transport
container
container
with 2.0-
2.5 ml of
Media
Sterile
proof
VTM

-
An adequate sample should be
Sample Volume
-
about 3-5ml
-
Sample type
specime
Oral or
buccal
Sputum
swab
Others
is in sequence.
n
Isolation
Method
qPCR
Microscopy for Acid fast bacilli (AFB) smear
Virus
(VI)/
Test
Note : Only for selected case

Outbreak
Category
For confirmation For confirmation

Pathogen
Mycobacterium
Mycobacterium tuberculosis
Mumps virus tuberculosis complex
complex (MTBC)
(MTBC)
Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
Diagnostic Sputum Clean leak Daily TB 1327/ √ √ √ √ √ To ensure optimal

complex (MTBC)
Mycobacterial Culture
about 3-5ml
An adequate sample should be
but not be frozen.

kept cool during transportation
4°C – 8°C. Specimens should be
TBIS 20C
49 (working days)
RM 20.00
proof 1328 recovery of AFB
container bacilli from
sputum, at least
two specimens
should be
collected and
processed for
mycobacterial
culture
Diagnostic Gastric Sterile Daily TB 1327/ √ √ √ √ √ Sample should be

Mycobacterium tuberculosis complex (MTBC)
Mycobacterial Culture
transportation but not be frozen.

4°C – 8°C. Specimens should be kept cool during
TBIS 20C
49 (working days)
RM 20.00
Minimum 5 ml
lavage Screw 1328 neutralise

capped immediately by
container. adding 1.5 ml
sterile 40%
anhydrous
disodium
phosphate
(Na2HPO4) for
every 35-40 ml
specimens if there
is futher delay for
more than 4 hours.
OR
Neutralize
specimen within 4
hours of collection
with 100 mg of
sodium carbonate
per 5 to 10 mL of
gastric washing.

Page 68
Remark(s)
MKA
K. Kinabalu
√
√
Public Health
Laboratories
MKA
√
Kota Bharu
MKA
√
Johor Bahru
√ √
√ √
√ √
MKA Ipoh
MKAK
Tel No. 03-
1327/
1327/
1327/
1328
1328
1328
61261200
Ext. No
Laboratory
TB
TB
TB
name
Frequency of
Daily
Daily
Daily
test
LTAT
49 (working days) 49 (working days) 49 (working days)

Type of
form
TBIS
TBIS
TBIS
20C
20C
20C
Sample 4°C – 8°C. Specimens should be 4°C – 8°C. Specimens should be 4°C – 8°C. Specimens should
Transportation kept cool during transportation kept cool during transportation be kept cool during transport-
but not be frozen. but not be frozen. tion but not be frozen.
Container/
Transport
container
container
capped
capped
Media
Sterile
Sterile
Screw
Screw

2-5 ml
As much as available
Sample Volume Minimum 5 ml (preferably minumum of 1
ml)
l washing
Bronchio
Sample type
Pleural,
Ascitic,
Serous
Perito-
neal)
Fluid
(eg.
CSF
is in sequence.
Method
Test
Mycobacterial Culture Mycobacterial Culture Mycobacterial Culture

Category
Pathogen
Mycobacterium tuberculosis Mycobacterium tuberculosis Mycobacterium tuberculosis
complex (MTBC) complex (MTBC) complex (MTBC)
Page 69
distilled water to
Remark(s)
prevent swab
sterile saline/
2. Add 1-2 ml
1.Should be
desiccation
aseptically
collected
MKA
K. Kinabalu
√
√
Public Health
Laboratories
MKA
√
Kota Bharu
MKA
√
Johor Bahru
√ √
√ √
√ √
MKA Ipoh
MKAK
Tel No. 03-
1327/
1327/
1327/
1328
1328
1328
61261200
Ext. No
Laboratory
TB
TB
TB
name
Frequency of
Daily
Daily
Daily
test
LTAT
49 (working days) 49 (working days) 49 (working days)

Type of
form
TBIS
TBIS
20C
20C
TBIS
20C
Sample 4°C – 8°C. Specimens should be 4°C – 8°C. Specimens should be 4°C – 8°C. Specimens should
Transportation kept cool during transportation kept cool during transportation be kept cool during
but not be frozen. but not be frozen. transportation but not be
frozen.
Container/
Transport
container
container
container
capped
capped
Media
Sterile
Sterile
Screw
Screw
capped
Sterile
Screw

2-5 ml
3-5 ml
Sample Volume -
any swab
synovial)
Sample type
morning
swabs or
urine)
Other
(early
Urine
fluids
body
(eg.
Pus or
is in sequence.
pus
Method
Test
Mycobacterial Culture Mycobacterial Culture Mycobacterial Culture

Category
Pathogen
Mycobacterium tuberculosis Mycobacterium tuberculosis complex (MTBC)
complex (MTBC) complex (MTBC)
Page 70
Add 2-5 ml sterile
water to prevent
saline/ distilled
Remark(s)
desiccation.
MKA
K. Kinabalu
√
√
Public Health
Laboratories
MKA
√
Kota Bharu
MKA
√
Johor Bahru
√ √
√ √
MKA Ipoh
MKAK
Tel No. 03-
1327/
1327/
1328
1328
61261200
Ext. No
Laboratory
TB
TB
name
Frequency of
Daily
Daily
test
LTAT
49 (working days) 49 (working days)

Type of
form
TBIS
TBIS
20C
20C
Sample 4°C – 8°C. Specimens should be kept 4°C – 8°C. Specimens should be kept cool during
Transportation cool during transportation but not be transportation but not be frozen.
frozen.
Container/
Transport
Media
Sterile Screw capped container without

culture
bottle
Blood
preservatives.

As large a sample as possible should be
Sample Volume Adult sample : 5-10 ml Peadiatric sample : 3-5 ml
sent
Sample type
Biopsy
Tissue
Blood
is in sequence.
Method
Test
Mycobacterial Culture Mycobacterial Culture

Category
Pathogen
Mycobacterium tuberculosis complex
(MTBC)
Page 71
Remark(s)
MKA
K. Kinabalu
√
√
Public Health
Laboratories
MKA
√
Kota Bharu
MKA
√
Johor Bahru
√ √
√ √
MKA Ipoh
MKAK
Tel No. 03-
1327/
1327/
1328
1328
61261200
Ext. No
Laboratory
TB
TB
name
Frequency of
Daily
Daily
test
LTAT

Type of
form
TBIS
TBIS
20C
20C
Sample 4°C – 8°C. Specimens should be kept cool 4°C – 8°C. Specimens should be kept cool
Transportation during transportation but not be frozen. during transportation but not be frozen.
Container/
Transport
specimen
culture
Media
As per
bottle
Blood
type

Adult sample : 5-10 ml Peadiatric sample : 3-5
Sample Volume As per specimen type
ml
Sample type
specime
mortem
Marrow
Bone
Post
n
is in sequence.
Method
Test
Mycobacterial Culture Mycobacterial Culture

Category
Pathogen
Mycobacterium tuberculosis complex (MTBC) Mycobacterium tuberculosis complex (MTBC)
Science Officer in-
Science Officer in-
Page 72
request to do this
request to do this
Microbiologist or
Microbiologist or
RR-TB and MDR-
RR-TB and MDR-

test method for
test method for

Remark(s)
Please consult
Please consult
MKA's Clinical
MKA's Clinical
charge if you
charge if you
TB.
TB.
MKA
K. Kinabalu
Public Health
Laboratories
MKA
Kota Bharu
MKA
Johor Bahru
MKA Ipoh
√
MKAK
Tel No. 03-
1327/
1327/
1328
1328
61261200
Ext. No
Laboratory
TB
TB
name
350.00
Cost/test (RM) RM 350.00

Frequency of
2x/week 2x/week
test
7 working days (external samples) 7 working days (external samples)
LTAT
7 working days from date of RR-TB or MDR-TB 7 working days from date of RR-TB or MDR-TB
detection (internal samples) detection
(internal samples)
Type of
form
TBIS
TBIS
20C
20C
Sample 4°C – 8°C. Specimens should be kept cool 4°C – 8°C. Specimens should be kept cool during
Transportation during transportation but not be frozen. transportation but not be frozen.
Container/
Clean leak
Transport
container
Clean leak
container
Media
proof
proof

Sample Volume An adequate sample should be about 3-5ml An adequate sample should be about 0.5 ml
Sample type
Sputum
Processes sediments for culture
is in sequence.
Method
Detection of MTB and assess resistance to Detection of MTB and assess resistance to
Test
fluoroquinolone (FQ) and secondline fluoroquinolone (FQ) and secondline injectable

injectable drugs (SLID) drugs (SLID)
Diagnostic &
Diagnostic &
Surveillance
Surveillance
Category
Pathogen
Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
Xpert Sputum Clean leak TBIS 350.00 TB 1327/ √ 1. Request limited

An adequate sample should be about 3-5ml
4°C – 8°C. Specimens should be kept cool during transportation but not be frozen.
1 (working days)
Daily (working days)

MTB/Rif proof 20C 1328 for high suspected
Assay container MDR-TB and
Paediatric cases
only
2. For specimens
other than sputum
such as CSF,
pleural fluid and
gastric lavage-
please consult
MKAK's Clinical
Microbiologist &
Science Officer in-
charge before
request is made.
Sputum Clean leak TBIS TB 1327/ √ √ √ √ √ 1.Request for high
(MTBC)
rifampicin and isoniazid

Detection of MTB and assess resistance to
5ml
An adequate sample should be about 3-
7 (working days)
2x/week
RM 350.00
proof 20C 1328 suspected MDR-TB
container and Paediatric
cases
2. For specimens
other than sputum
such as CSF,
pleural fluid and
gastric lavage-
please consult
MKAK's Clinical
Microbiologist &
Science Officer in-
charge before
request is made.

Page 74
Remark(s)
MKA
K. Kinabalu
√
√
Public Health
Laboratories
MKA
√
Kota Bharu
MKA
√
Johor Bahru
√ √
√ √
MKA Ipoh
MKAK
Tel No. 03-
1327/
1327/
1328
1328
61261200
Ext. No
Laboratory
TB
TB
name
Frequency of
2x/week 2x/week
test
LTAT

Type of
form
TBIS
TBIS
20C
20C
Sample 4°C – 8°C. Specimens should be

Transportation kept cool during transportation but
not be frozen.
Container/
Transport
container
container
capped
capped
Media
Sterile
Sterile
Screw
Screw

3-5 ml
Sample Volume minimum 5 ml

Sample type
Sputum
Gastric
lavage
is in sequence.
Method
Detection of M. tuberculosis Detection of M. tuberculosis

Test
Complex by PCR method Complex by PCR method

Category
Pathogen
Mycobacterium tuberculosis Mycobacterium tuberculosis
complex (MTBC) complex (MTBC)
Page 75
Remark(s)
MKA
K. Kinabalu
√
√
Public Health
Laboratories
MKA
√
Kota Bharu
MKA
√
Johor Bahru
√ √
√ √
MKA Ipoh
MKAK
Tel No. 03-
1327/
1327/
1328
1328
61261200
Ext. No
Laboratory
TB
TB
name
Frequency of
2x/week 2x/week
test
LTAT

Type of
form
TBIS
TBIS
20C
20C
Sample
Transportation
Container/
Transport
container
container
capped
Media
Sterile
Screw
capped
Sterile
Screw

As much as available
Sample Volume 1-2 ml (preferably minimum of
0.5 ml)
Sample type
CSF
Bronchiol washing
is in sequence.
Method
Detection of M.
Detection of M. tuberculosis
Test
tuberculosis Complex by
Complex by PCR method
PCR method
Category
Pathogen
Mycobacterium
tuberculosis complex
complex (MTBC)
(MTBC)
in sterile container
for swab. Only put
with sterile saline.
Page 76
media is needed
Remark(s)
No transport
MKA
K. Kinabalu
√
√
Public Health
Laboratories
MKA
√
Kota Bharu
MKA
√
Johor Bahru
√ √
√ √
MKA Ipoh
MKAK
Tel No. 03-
1327/
1327/
1328
1328
61261200
Ext. No
Laboratory
TB
TB
name
Frequency of
2x/week 2x/week
test
LTAT

Type of
form
TBIS 20C TBIS 20C

Container/
Transport
container
container
capped
capped
Media
Sterile
Sterile
Screw
Screw

1-2 ml
Sample Volume
-
Synovial)
Pus/ Pus
Sample type
neal) or
Pleural,
Ascitic,
Others
Serous
Perito-
swab/
other
fluids
swab
body
Fluid
(e.g.
(eg.
is in sequence.
Method
Detection of M. tuberculosis Complex by Detection of M. tuberculosis Complex by

Test
PCR method PCR method

Category
Pathogen
Mycobacterium tuberculosis complex Mycobacterium tuberculosis complex
(MTBC) (MTBC)
Page 77
Add 2-5 ml sterile
water to prevent
saline/ distilled
Remark(s)
desiccation
MKA
K. Kinabalu
√
√
Public Health
Laboratories
MKA
√
Kota Bharu
MKA
√
Johor Bahru
√ √
√ √
MKA Ipoh
MKAK
Tel No. 03-
1327/
1327/
1328
1328
61261200
Ext. No
Laboratory
TB
TB
name
Frequency of
2x/week 2x/week
test
LTAT

Type of
form
TBIS 20C TBIS 20C

Container/
Transport
container
capped
Media
Sterile
Screw

preservatives.

1-2 ml
Sample Volume As large a sample as possible should be sent

Sample type
Biopsy
Tissue
Urine
is in sequence.
Method
Detection of M. tuberculosis Complex by Detection of M. tuberculosis Complex by PCR

Test
PCR method method

Category
Pathogen
(MTBC)
Page 78
Remark(s)
MKA
K. Kinabalu
√
√
Public Health
Laboratories
MKA
√
Kota Bharu
MKA
√
Johor Bahru
√ √
√ √
MKA Ipoh
MKAK
Tel No. 03-
1327/
1327/
1328
1328
61261200
Ext. No
Laboratory
TB
TB
name
RM 350.00
Frequency of
2x/week 2x/week
test
LTAT

Type of
form
TBIS 20C TBIS 20C

Sample 4°C – 8°C. Specimens should be kept cool during 4°C – 8°C. Specimens should be kept cool
Transportation transportation but not be frozen. during transportation but not be frozen.
Container/
separator)
Plain tube
Transport
Heparin
Media
serum
(non-

tube
preservatives.

1-2 ml
Sample Volume Adult sample: 5 ml Paediatric sample: 3-5 ml
Sample type
Marrow
Blood
Bone
is in sequence.
Method
Detection of M. tuberculosis Complex by PCR Detection of M. tuberculosis Complex by

Test
method PCR method

Category
Pathogen
(MTBC)
weeks and exclude
from LTAT criteria.
contaminated/mix
Page 79
take another 2-8
need additional
procedure. This
Insufficient and
Remark(s)
procedure will
ed growth will
MKA
K. Kinabalu
√
√
Public Health
Laboratories
MKA
√
Kota Bharu
MKA
√
Johor Bahru
√ √
√ √
MKA Ipoh
MKAK
Tel No. 03-
1327/
1327/
1328
1328
61261200
Ext. No
Laboratory
TB
TB
name
Frequency of
2x/week Daily (working days)
test
3 (working days) for MTBC
LTAT
7 (working days) 35 (working days) for NTM

Type of
form
TBIS 20C TBIS 20C

Sample 4°C – 8°C. Specimens should be kept cool
Ambient temperature
Transportation during transportation but not be frozen.
Container/
Transport
container
capped
Media
Screw
As per specimen type

Visible growth on the egg-based slope (> 20
Sample Volume As per specimen type
colonies). Pure colonies on solid media
Sample type
specime
mortem
Post
Culture isolates- solid medium
n
is in sequence.
Method
Detection of M. tuberculosis Complex by PCR

Test
Culture Identification
method
Surveillance
Diagnostic
Category
Pathogen
The liquid medium
within 3 days after
microscopic result
Page 80
positive. Please
Remark(s)
on the request
should be sent
state the
form.
MKA
K. Kinabalu
√
Public Health
Laboratories
MKA
√
Kota Bharu
MKA
√
Johor Bahru
√ √
MKA Ipoh
MKAK
Tel No. 03-
1327/
1328
61261200
Ext. No
Laboratory
TB
name
RM 50.00
Cost/test (RM)
Frequency of
Daily (working days) Depends on quantity of sample
test
3 (working days) for MTBC
LTAT
35 (working days) for NTM

Type of
form
TBIS 20C
Sample
Ambient temperature
Transportation
Container/
e.g.: MGIT
Transport
container
capped
Media
Screw
tube

Visible growth on the egg-based slope (please ensure more
Sample Volume
than 20 colonies & pure growth)
Sample type
isolates-
medium
Culture
liquid
is in sequence.
Method
Test
Culture Identification
Surveillance
Category
Pathogen
The liquid medium
within 3 days after
microscopic result
Page 81
positive. Please
Remark(s)
on the request
should be sent
state the
form.
MKA
K. Kinabalu
√
√
Public Health
Laboratories
MKA
√
Kota Bharu
MKA
√
Johor Bahru
√ √
√ √
MKA Ipoh
MKAK
Tel No. 03-
1327/
1327/
1328
1328
61261200
Ext. No
Laboratory
TB
TB
name
RM 50.00
Frequency of Daily - Depends on
Daily (working days) (working quantity of
test
days) sample
LTAT

Type of
form
TBIS 20C TBIS 20C

Sample
Ambient temperature Ambient temperature
Transportation
Container/
e.g.: MGIT
Transport
container
container
capped
capped
Media
Screw
Screw
tube

Visible growth on the egg-based slope (please
Sample Volume ensure more than 20 colonies & pure growth) Pure growth in liquid culture
Sample type
isolates-
isolates-
medium
medium
Culture
Culture
liquid
solid
is in sequence.
Method
First-line Drug susceptibility

First-line Drug susceptibility testing (SM, INH,
Test
testing (SM, INH, RIF, ETB) for

RIF, ETB) for MTBC
MTBC
Surveillance
Surveillance
Category
Pathogen
complex (MTBC)
Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
Surveillance Culture Screw TB 1327/ √ √ √ √ √ 1.This test will be

MTBC
First-line Drug susceptibility testing (PZA) for
based slope (> 20 colonies)

Visible growth on the egg-
Ambient temperature
TBIS 20C
21(working days)
2x/week
RM 50.00
isolates capped 1328 done for INH
container or/and Rif
resistance cases
2.For other cases,

please consult
MKAK's Clinical
Microbiologist &
Science Officer in-
based medium
Pure colonies on egg
charge before
request is made.
Culture Screw TB 1327/ √ √ √ √ √ 1. This test will be

complex (MTBC)
testing (PZA) for MTBC

First-line Drug susceptibility
Pure growth in liquid culture
Ambient temperature
TBIS 20C
21(working days)
2x/week
RM 50.00
isolates capped 1328 done for INH
container or/and Rif
e.g.: MGIT resistance cases
tube 2. For other cases,
please consult
MKA's Clinical
Microbiologist or
Science Officer in-
charge before
request is made.

Science Officer in-
Page 83
2.For other cases,
1.This test will be
Microbiologist &
resistance cases
request is made
MKAK's Clinical
Remark(s)
please consult
charge before
done for INH
or/and Rif
MKA
K. Kinabalu
Public Health
Laboratories
MKA
Kota Bharu
MKA
Johor Bahru
MKA Ipoh
√
MKAK
Tel No. 03-
1327/
1328
61261200
Ext. No
Laboratory
TB
name
Frequency of
1x/week
test
LTAT
31 days form detection of RR-TB and MDR-TB (internal)

Type of
form
TBIS 20C
Sample
Ambient temperature
Transportation
Container/
Transport
container
capped
Media
Screw

Visible growth on the egg-based slope (> 20 colonies)
Sample Volume
Pure colonies on solid medium
Sample type
isolates-
medium
Culture
solid
is in sequence.
Method
Second-line Drug susceptibility testing for MTBC

Test
(phenotypic method)
Category
Pathogen
The liquid medium
within 3 days after
microscopic result
and MDR-TB case,
Science Officer in-

1. This test will be
done for RR-TB or
Page 84
Microbiologist or
2. For non-RR-TB
request is made.
positive. Please
Remark(s)
on the request
should be sent
please consult
charge before
MKA's Clinical
MDR-TB case
state the
form.
MKA
K. Kinabalu
Public Health
Laboratories
MKA
Kota Bharu
MKA
Johor Bahru
MKA Ipoh
√
MKAK
Tel No. 03-
1327/
1327/
1328
1328
61261200
Ext. No
Laboratory
TB
TB
name
RM 350.00
Frequency of
1x/week 1x/week
test
31 days form detection of RR-TB and MDR-TB 7 days (external samples)
LTAT
(internal) 7 days from date of RR-TB or MDR-TB detection

(internal samples)
Type of
form
TBIS 20C TBIS 20C

Sample
Transportation
Container/
e.g.: MGIT
Transport
container
container
capped
capped
Media
Screw
Screw
tube

Visible growth on the egg based
Sample Volume Pure growth in liquid culture slope (> 20 colonies)
Pure culture on egg based medium
Sample type
isolates-
isolates-
medium
medium
Culture
Culture
liquid
solid
is in sequence.
Method
Detection of MTB and assess resistance to

Second-line Drug susceptibility testing for MTBC
Test
fluoroquinolone (FQ) and secondline injectable

(phenotypic method)
drugs (SLID)
Category
Pathogen
The liquid medium
within 3 days after
microscopic result
Page 85
positive. Please
Remark(s)
on the request
should be sent
state the
form.
MKA
K. Kinabalu
Public Health
Laboratories
MKA
Kota Bharu
MKA
Johor Bahru
MKA Ipoh
√
MKAK
Tel No. 03-
1327/
1328
61261200
Ext. No
Laboratory
TB
name
Frequency of
1x/week
test
7 days (external samples)
LTAT
7 days from date of RR-TB or MDR-TB detection (internal samples)

Type of
form
TBIS 20C
Sample
Ambient temperature
Transportation
Container/
e.g.: MGIT
Transport
container
capped
Media
Screw
tube

Sample Volume Pure growth in liquid culture
Sample type
Culture isolates-liquid medium
is in sequence.
Method
Detection of MTB and assess resistance to fluoroquinolone (FQ)

Test
and second line injectable drugs (SLID)

Category
Pathogen
1 ml TB 1327/ √ i. Special collection,
Interferon gamma release assay (IGRA) for diagnosis latent TB infection (LTBI) (blood test)
Whole blood in QuantiFERON-Gold tubes
QuantiFERON-QTB Gold collection tubes
First condition & Second condition: See Remarks
TBIS 20C
As scheduled
1x/month
RM 30.00
per 1328 incubation, and
tube centrifugation
procedures must be
followed. First
Condition: Incubate
tubes at collection
site (upright at 37°C
for 16-24 hours)
then ship to lab
NPHL at 4-27°C.
Record as
"incubated".
Second Condition:
Ship tubes to NPHL
at 17-27°C (blood
must be incubated
at 37°C as soon as
possible and within
16 hours of
collection). Record
as "not incubated".
Please contact
MKAK for
QuantiFERON-Gold
collection tubes and
special instruction.
ii. By appointment
ONLY through
MKAK's Clinical
Microbiologist.
iii. ONLY for health

care workers (HCW)
at Ministry of
Health.

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
Outbreak/ Culture Screw TB 1327/ √ 1. Please consult

MIRU-VNTR Typing / Spoligotyping
Pure growth in liquid culture

Ambient temperature
TBIS 20C
As necessary
350.00
Cluster isolates- capped 1328 Science Officer in-
solid container charge before
medium/ request is made.
liquid 2. All the request
medium must be
accompanied by a
complete clinical
notes or details of
cluster/outbreak
report.
BCG BCG vial 2 vials Vial 4 - 8°C BCG 35 days TB 1289 √ √ √ √ √

(MTBC)
If cold chain is indicated
RM 50.00
Viability for request
and each form
Potency lot
Test

Page 88
Remark(s)
NIl
Nil
Nil
MKA
K. Kinabalu
Public Health
Laboratories
MKA
Kota Bharu
MKA
Johor Bahru
MKA Ipoh
√
MKAK
Tel No. 03-
61261200 03-61402474 (Direct line) 03-61402474 (Direct line)
Ext. No
Laboratory
Leprosy Leprosy
name
Frequency of
Daily (Working days) Daily (working days)
test
LTAT
12 – 15 months 7 working days

Type of
Mycobacterium leprae Viability & Drug

form
PER-PAT 301
Sensitivity Test Request Form
Sample Transport sample at 2-8 °C and to reach Sample in container without preservative should
Transportation laboratory within 24 hours after collection be transported to reach the laboratory at 2-8 °C
within 5 days after collection.
Container/
Transport
Media
a) Preferably in sterile plain container without

Sterile plain container without preservative
preservative

Minimum size of 4 Minimum of 5 mm
Sample Volume Minimum size of 4 mm x 12 mm
mm x 12 mm punch biopsy.
Sample type
Biopsy
Biopsy
Biopsy
Punch
Skin
Skin
is in sequence.
Method
Culture & Sensitivity PCR (Detection of Mycobacterium leprae using PCR

Test
(Mouse Foot Pad Inoculation Method) method)

Category
NIL
Pathogen
Mycobacterium leprae
transportation will
Page 89
affect the quality
1. Please refer to
Disease Division
sample shall be
Remark(s)
Procedures for
3. Haemolysed
of the sample.
Laboratory
2. Delay in
Collection
Specimen
rejected.
Testing.
Nil
MKA
K. Kinabalu
√
Public Health
Laboratories
MKA
Kota Bharu
MKA
Johor Bahru
MKA Ipoh
√
MKAK
Tel No. 03-
1329/
1330
61261200 03-61402474 (Direct line)

Ext. No
Laboratory
Leprosy Bacteriology
name
Frequency of
Daily
4th week of each month by batches

test
3 days
LTAT
14 working days
Type of
form
MKAK-BPU-U01
Sample Sample in container with 70% ethanol can Send immediately after collection
Transportation be stored for longer period of time before at 2°C – 8°C
delivery to the laboratory
Container/
Plain Tube
Transport
container
with 70%
ethanol
Media
b) In

3 ML
Sample Volume Minimum of 5 mm punch biopsy.
Sample type
Biopsy
Serum
Punch
is in sequence.
Serology
Method
PCR (Detection of drug resistant

Test
Mycobacterium leprae by Molecular Line

Probe Assay Method
Outbreak
Category
Pathogen
Mycoplasma pneumoniae
transportation will
Page 90
1. Please refer to
Disease Division
of the bacteria.
Remark(s)
Procedures for
Laboratory
2. Delay in
Collection
Specimen
Testing.
MKA
K. Kinabalu
√
Public Health
Laboratories
MKA
√
Kota Bharu
MKA
√
Johor Bahru
√ √
√ √
√ √
√ √
MKA Ipoh
MKAK
Tel No. 03-
1329/
1329/
1329/
1329/
1330
1330
1330
1330
61261200
Ext. No
Laboratory
name
Frequency of
Daily
Daily
Daily
Daily
test
5 days
5 days
5 days
5 days
LTAT
Type of
form

Send within 12 hours Send within 12 hours Send within 12 hours Send within 12 hours
Sample
after collection at after collection at after collection at after collection at
Transportation
ambient temperature ambient temperature ambient temperature ambient temperature
Container/
Transport
Charcoal
Charcoal
Charcoal
Charcoal
Media
Amies
Amies
Amies
Amies

NA
NA
NA
NA
Sample Volume
Sample type
Urethral
Cervical
Vaginal
Swab
Swab
Swab
High
Pharyngeal Swab
is in sequence.
Culture &
Sensitivit
Method
Test
y
Outbreak or
Surveillance
Category
Pathogen
Neisseria gonorrhoeae
transportation will
Page 91
1. Please refer to
Disease Division
of the bacteria.
Remark(s)
Procedures for
2. Delay in
Laboratory
Collection
Specimen
Testing.
MKA
K. Kinabalu
Public Health
Laboratories
MKA
√
Kota Bharu
MKA
√
Johor Bahru
√ √
√ √
√ √
MKA Ipoh
MKAK
Tel No. 03-
1329/
1329/
1329/
1330
1330
1330
61261200
Ext. No
Laboratory
name
Frequency of
Daily
Daily
Daily
test
5 days
5 days
5 days
LTAT
Type of
form

Send within 12 hours Send within 24 hours after collection Send within 24 hours after collection at
Sample
after collection at at ambient temperature. If delay is ambient temperature. If delay is
Transportation
ambient temperature unavoidable, send at 2°C – 8°C unavoidable, send at 2°C – 8°C
Container/
Transport
Charcoal
Charcoal
Charcoal
Media
Amies
Amies
Amies

NA
NA
NA
Sample Volume
(contact)
Sample type
Throat
swab
Conjunctivae Swab Nasopharyngeal swab (contact)
is in sequence.
Culture &
Sensitivit
Method
Test
y
Outbreak or
Diagnostic
Category
Pathogen
Neisseria meningitidis
Page 92
Remark(s)
MKA
K. Kinabalu
Public Health
Laboratories
MKA
√
Kota Bharu
MKA
√
Johor Bahru
√ √
√ √
√ √
MKA Ipoh
MKAK
Tel No. 03-
1329/
1329/
1329/
1330
1330
1330
61261200
Ext. No
Laboratory
name
Frequency of
Daily Daily Daily
test
LTAT
5 days 5 days 5 days

Type of
form

Sample Send within 24 hours Send within 24 hours Send within 24 hours after collection
Transportation after collection after collection at 2°C – 8°C.
at 2°C – 8°C. at 2°C – 8°C.
Container/
Transport
container
container
container
Media
Sterile
Sterile
Sterile

1-3
1-3
1-3
ML
ML
ML
Sample Volume
Sample type Sputum
(Preference of sample Nasopharyngeal Aspirate
Tracheal Aspirates (in-patient)
is in sequence. (in-patient)
Method
Test
Category
Pathogen
transportation will
Page 93
1. Please refer to
Disease Division
than 1 hour not
of the bacteria.
Remark(s)
Procedures for
received more
CSF which is
suitable for
2. Delay in
Laboratory
Collection
Specimen
culturing.
Testing.
MKA
K. Kinabalu
Public Health
Laboratories
MKA
√
Kota Bharu
MKA
√
Johor Bahru
√ √
√ √
MKA Ipoh
√
MKAK
Tel No. 03-
1329/
1329/
1329/
1329/
1330
1330
1330
1330
61261200
Ext. No
Laboratory
name
Frequency of
Daily
Daily
Daily
Daily
test
5 days
5 days
2 days
2 days
LTAT
Type of
form

Sample Send within 24 hours Send within 1 hour Send immediately Send immediately
Transportation after collection after collection after collection after collection
at 2°C – 8°C. at 2°C – 8°C. at 2°C – 8°C. at 2°C – 8°C.
Container/
Plain Tube
Transport
container
container
container
Media
Sterile
Sterile
Sterile

1 ML
1 ML
3 ML
1-3
ML
Sample Volume
Sample type
patient)
CSF (in-
Serum
Bronchoalveolar lavage
CSF
is in sequence. (BAL) (in-patient)
Method
Test
PCR for Neisseria meningitidis

Category
Pathogen
Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
Plasma 3 ML EDTA Tube 2 days Daily 1329/ √
MKAK-BPU-U01
RM 350.00
Bacteriology
at 2°C – 8°C.
1330
Surveillance Isolate NA Blood Agar Send at 3 days Daily 1329/ √ Inoculate pure
Serotyping
MKAK-BPU-U01
RM 20.00
Bacteriology
2°C – 1330 single colony onto
8°C. Blood Agar.
Outbreak Isolate NA Blood Agar Send at 30 days 1329/ √ Test will be done
Electrophoresis
Pulsed-field Gel
MKAK-BPU-U01
Requirement
Special
RM 350.00
Bacteriology
2°C – 1330 for outbreak
8°C. investigation and
epidemiological
linkage only.
Surveillance Isolate NA Blood Agar Send at 30 days 1329/ √ Test will be done
Typing
Sequence
Multilocus
MKAK-BPU-U01
Requirement
Special
RM 350.00
Bacteriology
2°C – 1330 for outbreak
8°C. investigation and
epidemiological
linkage only.

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
Nipah Outbreak qPCR/ CSF 1-3 ml Sterile 2-8°C √ 1. Sample should
MKAK-BPU-U01
EM - 2 days
qPCR - 3 days
EM - Daily
qPCR- Daily
EM - RM 15.00
qPCR-RM257.00
Virology
EM Unit 1320 / 1335

Molecular Unit 1321
virus EM screw be collected within
2. A brief concise
history of illness
and physical
with serum
separator
especially the date
of onset of illness
Urine Sterile
morning first void)
10 ml of urine (Early
and date of sample
screw
collection.
capped
container
Throat - Sterile
swab container
with 2.0-
2.5 ml of
VTM
Tissue 1.5 cm Sterile
biopsy / cube Screw
autopsy in a capped
few container
drops
of
VTM

The liquid medium
within 3 days after
microscopic result
Page 96
positive. Please
Remark(s)
on the request
should be sent
state the
form.
MKA
K. Kinabalu
Public Health
Laboratories
MKA
Kota Bharu
MKA
Johor Bahru
MKA Ipoh
√
MKAK
Tel No. 03-
1288
1327
61261200
Ext. No
Laboratory
NTM
NTM
name
Frequency of 2x/week for conventional method
1x/week for NTM speciation
test 1x/week for NTM speciation
35 days (NTM Runyon Group) 10 days (NTM
LTAT
10 days (NTM speciation)

speciation)
Type of
form
TBIS 20C TBIS 20C

Sample
Transportation
Container/
e.g.: MGIT
Transport
container
container
capped
capped
Media
Screw
Screw
tube

Sample Volume Pure colonies on solid medium Pure growth in liquid culture
Sample type
isolates-
isolates-
medium
medium
Culture
Culture
liquid
solid
is in sequence.
Method
Test
Culture Identification Culture Identification

Category
Pathogen
Non-tuberculous Mycobacterium (NTM) Non-tuberculous Mycobacterium (NTM)
and date of sample
be collected within
especially the date

Science Officer in-
5 days after onset
Page 97
of onset of illness
2. A brief concise
1. Sample should
1. Please consult
request is made.
history of illness
Microbiology or
MKAK’s Clinical
Remark(s)
charge before
and physical
collection.
of illness.
MKA √ (RAT only)

K. Kinabalu
Public Health
Laboratories
MKA √ (RAT only)

Kota Bharu
MKA √ (RAT only)
Johor Bahru
MKA Ipoh √ (RAT only)
√
MKAK
Tel No. 03- VI Unit 1315/1325
1288
61261200 EM Unit 1320 / 1335

Ext. No Molecular Unit 1321
Laboratory
NTM
Virology
name
RAT-RM30.00
Cost/test (RM) RM 50.00 EM - RM 15.00
PCR-RM214.30
Frequency of RAT- Daily
1x/week EM-Daily
test
PCR-Daily
7 days for rapid grower NTM from date of request. RAT - 24 hours
LTAT
21 days for slow grower NTM from date of request. EM - 2 days

PCR - 5 days
Type of
form
TBIS 20C MKAK-BPU-U01

Sample 2-8°C
Ambient temperature
Transportation
Container/
Transport
container
container
container
with 2.0-
2.5 ml of
capped
capped
Media
Sterile
Sterile
Screw
Screw
VTM

1.5 cm
5 ml /
cube
Sample Volume
-
Pure culture on solid medium
Sample type
isolates-
medium
Culture
Rectal
Fresh
Swab
stool
solid
is in sequence.
Method
RAT/
EM/
Test
PCR
PCR
Drug susceptibility testing for NTM
Category
Outbreak
Pathogen
Norovirus
Non-tuberculous Mycobacterium (NTM)
Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
Outbreak EM Fresh 5 ml / Sterile 2-8°C √ 1. Sample should

Orthoreovirus
MKAK-BPU-U01
EM - 2 days
EM-Daily
EM - RM 15.00
Virology
EM Unit 1320 / 1335

stool 1.5 cm container be collected within
cube with 2.0- 5 days after onset
2.5 ml of of illness.
VTM
2. A brief concise
history of illness
and physical
especially the date
of onset of illness
and date of sample
collection.
Orthopox Outbreak / EM Vesicle 0.5 -1 Sterile 2-8°C √ 1. Sample should
MKAK-BPU-U01
EM - 2 days
EM-Daily
EM - RM 15.00
Virology
EM Unit 1320 / 1335

virus Diagnostic fluid ml screw- be collected within
2. A brief concise
history of illness
and physical
especially the date
of onset of illness
and date of sample
collection.

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
Parapox Outbreak / EM Vesicle 0.5 -1 Sterile 2-8°C √ 1. Sample should
MKAK-BPU-U01
EM - 2 days
EM-Daily
EM - RM 15.00
Virology
EM Unit 1320 / 1335

Virus Diagnostic fluid ml screw- be collected within
2. A brief concise
history of illness
and physical
especially the date
of onset of illness
and date of sample
collection.
Outbreak IFAT / Sputum 1-3 ml Sterile 2-8°C √ 1. Sample should
Parainfluenza virus 1/2/3
MKAK-BPU-U01
VI - 21 days
IFAT - 7 days
VI - Daily
IFAT - Daily
VI - RM 75.00
IFAT - RM 35.00
Virology
VI Unit 1315/1325
screw be collected within
Virus capped 5 days after onset
Isolation Nasopha 1-3 ml Sterile of illness.
(VI) ryngeal screw
Aspirate capped 2. A brief concise
Throat - Sterile history of illness
Virus swab container and physical
Isolation with 2.0- findings is required
(VI) 2.5 ml of especially the date
Nasal - Sterile and date of sample
Pharynge container collection.
al Swab with 2.0-
2.5 ml of
VTM
Nasal - Sterile
Swab container
with 2.0-
2.5 ml of
VTM
Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media

/ endo- screw
tracheal capped
tube
Aspirate
alveolar screw
lavage capped
Lungs 1.5cm Sterile
tissue cube screw
autopsy few
drops
of
VTM
Outbreak PCR Serum 1-3 ml Plain tube 2-8°C √ 1. Sample should
Parvovirus B19
PCR - 5 days
PCR- Daily
PCR-RM210.20
Virology
Molecular Unit 1321

with serum be collected within
of illness.
CSF 1 ml Sterile
especially the date
of onset of illness
and date of sample
collection.

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media

Plesiomonas shigelloides
at 2°C – 8°C
MKAK-BPU-U01
RM 70.00
Bacteriology
Collection
Procedures for
Disease Division
Laboratory
Testing.
2. A sample which
is received more
than 2 hours will
be rejected.
unavoidable, send at 2°C – 8°C

MKAK-BPU-U01
RM 70.00
Bacteriology
stool 1330 Specimen
Collection
Procedures for
Disease Division
Laboratory
Testing.
2. Delay in
transportation will
of the bacteria.

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media

MKAK-BPU-U01
RM 70.00
Bacteriology
swab 1330 Specimen
Collection
Procedures for
Disease Division
Laboratory
Testing.
2. Ensure the swab

shows some faecal
staining to avoid
low quality of
sampling.
3. Delay in
transportation will
of the bacteria.
Outbreak Sputum 1-3 Sterile 5 days Daily 1329/ √ √ √ √ √ 1. Please refer to
Pseudomonas aeruginosa
at 2°C – 8°C.
collection
MKAK-BPU-U01
RM 70.00
Bacteriology
ML container 1330 Specimen
Collection
Procedures for
Disease Division
Laboratory
Testing.
2. Delay in
Tracheal 1-3 Sterile 5 days Daily 1329/ √ √ √ √ √ transportation will
at 2°C – 8°C.
after collection
Send immediately
MKAK-BPU-U01
RM 70.00
Bacteriology
Aspirates ML container 1330 affect the viability
(in- of the bacteria.
patient)

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
Broncho- 1-3 Sterile 5 days Daily 1329/ √ √ √ √ √
at 2°C – 8°C.
after collection
Send immediately
MKAK-BPU-U01
RM 70.00
Bacteriology
alveolar ML container 1330
lavage
(BAL) (in-
patient)
Outbreak IFAT/qPC Saliva 1-3 ml Sterile 2-8°C √ 1. Sample should

Rabies virus
MKAK-BPU-U01
IFAT - 7 days
qPCR - 3 days
IFAT- Daily
qPCR-Daily
IFAT-RM 35.00
qPCR -RM257.00
Virology
VI Unit 1315/1325
Molecular Unit 1321
R Screw be collected within
2. A brief concise
Skin 1-3 ml Sterile
history of illness
qPCR biopsy of Screw
and physical
the hair capped
follicles container
especially the date
(at the
of onset of illness
nap of
and date of sample
the neck)
collection.
Brain 1.5cm Sterile

autopsy cube Screw
in a capped
few container
drops
of
VTM

transportation will
Page 104
1. Please refer to
Disease Division
of the bacteria.
Remark(s)
Procedures for
2. Delay in
Laboratory
Collection
Specimen
Testing.
MKA
K. Kinabalu
Public Health
Laboratories
MKA
√
Kota Bharu
MKA
√
Johor Bahru
√ √
√ √
MKA Ipoh
MKAK
Tel No. 03-
1329/
1329/
1330
1330
61261200
Ext. No
Laboratory
name
Frequency of
Daily
Daily
test
5 days
5 days
LTAT
Type of
form
Sample Send within 24 hours after collection Send within 24 hours after collection at
Transportation at ambient temperature. If delay is unavoidable, ambient temperature. If delay is unavoidable,
send at 2°C – 8°C send at 2°C – 8°C
Container/
Transport
Charcoal
Charcoal
Media
Amies
Amies

NA
NA
Sample Volume
(contact)
(contact)
Nasopha
Sample type
ryngeal
Throat
swab
swab
is in sequence.
Culture &
Sensitivit
Method
Test
y
Outbreak or
Diagnostic
Category
Pathogen
Respiratory Bacterial Pathogens
Page 105
Remark(s)
MKA
K. Kinabalu
Public Health
Laboratories
MKA
√
Kota Bharu
MKA
√
Johor Bahru
√ √
√ √
√ √
√ √
MKA Ipoh
MKAK
Tel No. 03-
1329/
1329/
1329/
1329/
1330
1330
1330
1330
61261200
Ext. No
Laboratory
name
Frequency of
Daily
Daily
Daily
Daily
test
5 days
5 days
5 days
5 days
LTAT
Type of
form

Sample Send within 24 hours Send within 24 hours Send within 24 hours Send within 24
Transportation after collection at 2°C after collection after collection hours after
– 8°C. at 2°C – 8°C. at 2°C – 8°C. collection
at 2°C – 8°C.
Container/
Transport
container
container
container
container
Media
Sterile
Sterile
Sterile
Sterile

1-3
1-3
1-3
1-3
ML
ML
ML
ML
Sample Volume
Aspirates
Broncho-
(BAL) (in-
Nasopha
Sample type
Tracheal
Aspirate
patient)
patient)
alveolar
patient)
Sputum
ryngeal
lavage
(in-
(in-
is in sequence.
Method
Test
Category
Pathogen
Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
CSF (in- 1 ML Sterile 5 days Daily 1329/ √ √ √ √ CSF which is
at 2°C – 8°C.
after collection
Send within 1 hour
MKAK-BPU-U01
RM 70.00
Bacteriology
patient) container 1330 received more
than 1 hour not
suitable for
culturing.
Outbreak IFAT / Sputum 1-3 ml Sterile 2-8°C √ 1. Sample should

Respiratory syncytial virus
MKAK-BPU-U01
PCR - 5 days
IFAT - 7 days
PCR-Daily
IFAT-Daily
PCR-RM214.30
IFAT-RM 35.00
Virology
Molecular Unit 1321

VI Unit 1315/1325
√ (IFAT Only)
√ (IFAT Only)
√ (IFAT Only)
√ (IFAT Only)
PCR screw be collected within
ryngeal screw 2. A brief concise
Endotrac 1-3 ml Sterile collection.
heal screw
tube capped
aspirate container
alveolar screw
lavage capped
container
Throat - Sterile
swab container
with 2.0-
2.5 ml of
VTM

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
Nasal - Sterile
Pharynge container
al Swab with 2.0-
2.5 ml of
VTM
Nasal - Sterile
Swab container
with 2.0-
2.5 ml of
VTM
Lungs 1.5cm Sterile
tissue cube screw
in a capped
few container
drops
of
VTM
Rhinovirus Outbreak IFAT Sputum 1-3 ml Sterile 2-8°C √ 1. Sample should
MKAK-BPU-U01
VI - 21 days
IFAT - 7 days
VI - Daily
IFAT - Daily
VI - RM 75.00
IFAT - RM 35.00
Virology
VI Unit 1315/1325
√ (IFAT Only)
√ (IFAT Only)
√ (IFAT Only)
√ (IFAT Only)
screw be collected within
Nasopha 1-3 ml Sterile of illness.
ryngeal screw
Aspirate capped 2. A brief concise
Virus Throat - Sterile history of illness
Isolation swab container and physical
(VI) with 2.0- findings is required
2.5 ml of especially the date
Nasal - Sterile and date of sample
Phalange container collection.
al Swab with 2.0-
2.5 ml of
VTM

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
Nasal - Sterile
Swab container
with 2.0-
2.5 ml of
VTM
/ endo- screw
tracheal capped
tube
Aspirate
alveolar screw
lavage capped
Lungs 1.5cm Sterile
tissue cube screw
autopsy few
drops
of
VTM
Outbreak / Serology Serum 1-3 ml Plain tube 2-8°C √ 1. Sample should
Rubella virus
VI - 21 days
qPCR - 3 days
Serology-7 days
VI-Daily
qPCR- Daily
Serology -Daily
VI-RM 75.00
qPCR-RM257.00
Serology -RM61.34
Virology
VI Unit 1315/1325
Molecular Unit 1321
Serology Unit 4027
√ (Serology only)
MSLF:01/2004
Surveillance with serum be collected within
of illness.
qPCR/Vir Urine Sterile
morning first void)
10 ml of urine (Early
2. A brief concise
us screw
history of illness
Isolation capped
and physical
container
especially the date
of onset of illness
and date of sample
collection.

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
Throat - Sterile
swab container
with 2.0-
2.5 ml of
VTM

ryngeal screw
secretion capped
container

aspirate screw
capped
container
Rotavirus Outbreak RAT / Fresh 5 ml / Sterile 2-8°C √ 1. Sample should
MKAK-BPU-U01
EM - 2 days
PCR - 5 days
RAT - 24 hours
EM-Daily
PCR-Daily
RAT -Daily
EM - RM 15.00
PCR-RM214.30
RAT-RM30.00
Virology
EM Unit 1320 / 1335

Molecular Unit 1321
VI Unit 1315/1325
√ (RAT only)
√ (RAT only)
√ (RAT only)
√ (RAT only)
PCR/ stool 1.5 cm screw be collected within
EM cube capped 5 days after onset
PCR Rectal - Sterile 2. A brief concise

VTM especially the date
of onset of illness
and date of sample
collection.

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media

Salmonella sp.
at 2°C – 8°C.
MKAK-BPU-U01
RM 22.14
Bacteriology
and stool gm container 1330 Specimen
Procedures for
Disease Division
Laboratory
Testing.
2. A sample which
is received more
than 2 hours will
be rejected.

MKAK-BPU-U01
RM 22.14
Bacteriology
stool 1330 Specimen
Collection
Procedures for
Disease Division
Laboratory
Testing.
2. Delay in
transportation will
of the bacteria.

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
8°C
temperature. If delay is unavoidable, send at 2°C –
MKAK-BPU-U01
RM 22.14
Bacteriology
Swab 1330 Specimen
Collection
Procedures for
Disease Division
Laboratory
Testing.
2. Ensure the swab

shows some faecal
staining to avoid
low quality of
sampling.
3. Delay in
transportation will
of the bacteria.
Bacterial NA slant Send at Daily 1329/ √ √ Inoculate pure
Full Serotyping
Pure isolate
MKAK-BPU-U01
60 days (Surveillance)
21 days (Outbreak)
RM 175.17
Bacteriology
Culture 2°C – 1330 single colony onto
8°C Nutrient Agar.
Bacterial NA slant Send at 30 days 1329/ √ √ Test will be done

Electrophoresis
Pulsed-field Gel
Pure isolate
MKAK-BPU-U01
Requirement
Special
RM 350.00
Bacteriology
Culture 2°C – 1330 for outbreak
epidemiological
linkage only.

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
Bacterial NA slant Send at 30 days 1329/ √ Test will be done
Typing
Sequence
Multilocus
Pure isolate
U01
MKAK-BPU-
Requirement
Special
RM 350.00
Bacteriology
epidemiological
linkage only.

Salmonella Typhi
at 2°C – 8°C.
MKAK-BPU-U01
RM 70.00
Bacteriology
Collection
Procedures for
Disease Division
Laboratory
Testing.
2. A sample which
is received more
than 2 hours will
be rejected.

MKAK-BPU-U01
RM 70.00
Bacteriology
stool 1330 Specimen
Collection
Procedures for
Disease Division
Laboratory
Testing.
2. Delay in
transportation will
of the bacteria.

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media

MKAK-BPU-U01
RM 70.00
Bacteriology
swab 1330 Specimen
Collection
Procedures for
Disease Division
Laboratory
Testing.
2. Ensure the swab

shows some faecal
staining to avoid
low quality of
sampling.
3. Delay in
transportation will
of the bacteria.
Outbreak or Real- Fresh 3-5 Sterile 2 days Daily 1329/ √ √ √ √ √ 1. Please refer to
at 2°C – 8°C.
MKAK-BPU-U01
RM 350.00
Bacteriology
Surveillance Time PCR stool gm Container 1330 Specimen
(food Collection
handlers) Procedures for
Disease Division
Laboratory
Testing.
2. A sample which
is received more
than 2 hours will
be rejected.

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
at 2°C – 8°C.
MKAK-BPU-U01
RM 350.00
Bacteriology
stool 1330 Specimen
Collection
Procedures for
Disease Division
Laboratory
Testing.
2. Delay in
transportation will
of the bacteria.
at 2°C – 8°C.
MKAK-BPU-U01
RM 350.00
Bacteriology
Swab 1330 Specimen
Collection
Procedures for
Disease Division
Laboratory
Testing.
2. Ensure the swab

shows some faecal
staining to avoid
low quality of
sampling.
Outbreak Bacterial NA slant Send at 30 days 1329/ √ √
Electrophoresis
Pulsed-field Gel
Pure isolate
MKAK-BPU-U01
Requirement
Special
RM 350.00
Bacteriology
Culture 2°C – 1330 3. Delay in
8°C transportation will
of the bacteria.

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
Surveillance Multilocu Bacterial Pure NA slant Send at 30 days 1329/ √
MKAK-BPU-U01
Requirement
Special
RM 350.00
Bacteriology
s Culture isolate 2°C – 1330
Sequenc 8°C
e Typing
Surveillance Full Isolate NA NA slant Send at 21 days Daily 1329/ √ √ √ √ √ Inoculate pure
MKAK-BPU-U01
RM 350.00
Bacteriology
Serotypin 2°C – 1330 single colony onto
g 8°C Nutrient Agar.
Sapovirus Outbreak PCR/ Fresh 5 ml / Sterile 2-8°C √ 1. Sample should
MKAK-BPU-U01
PCR - 5 days
EM - 2 days
PCR-Daily
EM-Daily
EM - RM 15.00
PCR-RM214.30
Virology
EM Unit 1320/ 1335

Molecular Unit 1321
EM stool 1.5 cm screw be collected within
cube capped 5 days after onset
PCR Rectal - Sterile 2. A brief concise

VTM especially the date
of onset of illness
and date of sample
collection.

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
Shigella sp.
at 2°C – 8°C.
MKAK-BPU-U01
RM 70.00
Bacteriology
y Collection
Procedures for
Disease Division
Laboratory
Testing.
2. A sample which
is received more
than 2 hours will
be rejected.

MKAK-BPU-U01
RM 70.00
Bacteriology
stool 1330 Specimen
Collection
Procedures for
Disease Division
Laboratory
Testing.
2. Delay in
transportation will
of the bacteria.

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media

MKAK-BPU-U01
RM 70.00
Bacteriology
Swab 1330 Specimen
Collection
Procedures for
Disease Division
Laboratory
Testing.
2. Ensure the swab

shows some faecal
staining to avoid
low quality of
sampling.
3. Delay in
Full Bacterial NA slant Send at 7 days Daily 1329/ √ √ transportation will
Pure isolate
MKAK-BPU-U01
RM20.00
Bacteriology
Serotypin Culture 2°C – 1330 affect the viability
g 8°C. of the bacteria.
Outbreak Culture & Fresh 3-5 Sterile 5 days Daily 1329/ √ √ √ √ √

Pathogen)
Staphylococcus aureus (Enteric
at 2°C – 8°C.
MKAK-BPU-U01
RM 46.66
Bacteriology
Sensitivit stool gm container 1330
y

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media

MKAK-BPU-U01
RM 46.66
Bacteriology
stool 1330 Specimen
Collection
Procedures for
Disease Division
Laboratory
Testing.
2. Delay in
transportation will
of the bacteria.

MKAK-BPU-U01
RM 46.66
Bacteriology
Swab 1330 Specimen
Collection
Procedures for
Disease Division
Laboratory
Testing.
2. Ensure the swab

shows some faecal
staining to avoid
low quality of
sampling.
3. Delay in
transportation will
of the bacteria.

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
Toxin Bacterial NA slant Send at 7 days Daily 1329/ √ √ √ √ √
Pure isolate
MKAK-BPU-U01
RM 100.40
Bacteriology
Detectio Culture 2°C – 1330
n 8°C
Pulsed- Bacterial NA slant Send at 30 days 1329/ √ √
Pure isolate
MKAK-BPU-U01
Requirement
Special
RM 350.00
Bacteriology
field Gel Culture 2°C – 1330
Electroph 8°C
oresis
Outbreak Culture & NA Amies clear 5 days Daily 1329/ √ √ √ √ √ 1. Please refer to
Staphylococcus aureus (Respiratory Pathogen)
Nasal swab (MRSA contact screening)

MKAK-BPU-U01
RM 70.00
Bacteriology
Sensitivit 1330 Specimen
y Collection
Procedures for
Disease Division
Laboratory
Testing.
2. Delay in
transportation will
of the bacteria.

Page 120
Remark(s)
MKA
K. Kinabalu
√
√
Public Health
Laboratories
MKA
√
Kota Bharu
MKA
√
Johor Bahru
√ √
√ √
MKA Ipoh
MKAK
Tel No. 03-
1329/
1329/
1330
1330
61261200
Ext. No
Laboratory
name
Frequency of
Daily
Daily
test
5 days
5 days
LTAT
Type of
form
Transportation at ambient temperature. If delay is at ambient temperature. If delay is
unavoidable, send at 2°C – 8°C unavoidable, send at 2°C – 8°C
Amies clear
Amies clear
Container/
Transport
Media

NA
NA
Sample Volume
Nasopha
Sample type
ryngeal
Throat
swab
swab
is in sequence.
Method
Test
Category
Pathogen
Page 121
Remark(s)
MKA
K. Kinabalu
√
√
Public Health
Laboratories
MKA
√
Kota Bharu
MKA
√
Johor Bahru
√ √
√ √
MKA Ipoh
MKAK
Tel No. 03-
1329/
1329/
1330
1330
61261200
Ext. No
Laboratory
name
Frequency of
Daily
Daily
test
5 days
5 days
LTAT
Type of
form
Transportation at 2°C – 8°C at 2°C – 8°C
Amies clear
Container/
Transport
container
Media
Sterile

1-3
1-3
ML
ML
Sample Volume
Aspirates
Nasopha
Sample type
patient)
Sputum
ryngeal
(in-
is in sequence.
Method
Test
Category
Pathogen
Page 122
Remark(s)
MKA
K. Kinabalu
√
√
Public Health
Laboratories
MKA
√
Kota Bharu
MKA
√
Johor Bahru
√ √
√ √
MKA Ipoh
MKAK
Tel No. 03-
1329/
1329/
1330
1330
61261200
Ext. No
Laboratory
name
Frequency of
Daily
Daily
test
5 days
5 days
LTAT
Type of
form
Transportation at 2°C – 8°C at 2°C – 8°C
Container/
Transport
container
container
Media
Sterile
Sterile

1-3
1-3
ML
ML
Sample Volume
Aspirates
Broncho-
Sample type
Tracheal
alveolar
lavage
(BAL)
is in sequence.
Method
Test
Category
Pathogen
Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
Bacterial NA slant Send at 30 days 1329/ √ √ Test will be done
Electrophoresis
Pulsed-field Gel
Pure isolate
MKAK-BPU-U01
Requirement
Special
RM 350.00
Bacteriology
epidemiological
linkage only.
Outbreak Culture & Sputum 1-3 Sterile 5 days Daily 1329/ √ √ √ √ √ 1. Please refer to
Stenotrophomonas maltophilia
at 2°C – 8°C
Send 24 hours after collection
MKAK-BPU-U01
RM 70.00
Bacteriology
Sensitivit ML container 1330 Specimen
y Collection
Procedures for
Disease Division
Laboratory
Testing.
2. Delay in
transportation will
of the bacteria.
Tracheal 1-3 Sterile 5 days Daily 1329/ √ √ √ √ √
at 2°C – 8°C
Send 24 hours after collection
MKAK-BPU-U01
RM 70.00
Bacteriology
Aspirates ML container 1330

transportation will
Page 124
1. Please refer to
Disease Division
of the bacteria.
Remark(s)
Procedures for
2. Delay in
Laboratory
Collection
Specimen
Testing.
MKA
K. Kinabalu
√
√
Public Health
Laboratories
MKA
√
Kota Bharu
MKA
√
Johor Bahru
√ √
√ √
√ √
MKA Ipoh
MKAK
Tel No. 03-
1329/
1329/
1330
1330
1329/
1330
61261200
Ext. No
Laboratory
name
Frequency of
Daily
Daily
Daily
test
5 days
5 days
5 days
LTAT
Type of
MKAK-
form
BPU-
U01
Sample Send 24 hours Send within 24 hours after collection Send within 24 hours after collection
Transportation after collection at ambient temperature. If delay is at ambient temperature. If delay is
at 2°C – 8°C unavoidable, send at 2°C – 8°C unavoidable, send at 2°C – 8°C
Container/
Transport
container
Charcoal
Charcoal
Media
Sterile
Amies
Amies

1-3
ML
NA
NA
Sample Volume
Broncho-
(BAL) (in-
Nasopha
Sample type
alveolar
patient)
ryngeal
Throat
lavage
swab
swab
is in sequence.
Sent either one only) Culture &
Sensitivit
Method
Test
y
Outbreak
Category
Pathogen
Beta Haemolytic Streptococcus
transportation will
Page 125
1. Please refer to
Disease Division
of the bacteria.
Remark(s)
Procedures for
2. Delay in
Laboratory
Collection
Specimen
Testing.
MKA
K. Kinabalu
√
√
Public Health
Laboratories
MKA
√
Kota Bharu
MKA
√
Johor Bahru
√ √
√ √
√ √
MKA Ipoh
MKAK
Tel No. 03-
1329/
1329/
1329/
1330
1330
1330
61261200
Ext. No
Laboratory
name
Frequency of
Daily
Daily
Daily
test
5 days
5 days
5 days
LTAT
Type of
form

Sample Send within 24 hours Send within 24 hours Send within 24 hours after collection
Transportation after collection after collection at ambient temperature. If delay is
at 2°C – 8°C at 2°C – 8°C unavoidable, send at 2°C – 8°C
Container/
Transport
Container
Container
Charcoal
Media
Sterile
Sterile
Amies

1-3
1-3
ML
ML
NA
Sample Volume
Aspirates
(contact)
Nasopha
Sample type
swab or
Sputum
ryngeal
Throat
is in sequence.
Culture &
Sensitivit
Method
Test
y
Outbreak
Category
Pathogen
Streptococcus pneumoniae
Page 126
Remark(s)
MKA
K. Kinabalu
Public Health
Laboratories
MKA
√
Kota Bharu
MKA
√
Johor Bahru
√ √
√ √
√ √
MKA Ipoh
MKAK
Tel No. 03-
1329/
1329/
1329/
1330
1330
1330
61261200
Ext. No
Laboratory
name
Frequency of
Daily
Daily
Daily
test
5 days
5 days
5 days
LTAT
Type of
form

Sample Send within 24 hours after collection Send within 24 hours Send within 24 hours
Transportation at ambient temperature. If delay is after collection after collection
unavoidable, send at 2°C – 8°C at 2°C – 8°C at 2°C – 8°C
Container/
Transport
container
container
Charcoal
Media
Sterile
Sterile
Amies

1 -3
1 -3
ML
ML
NA
Sample Volume
Aspirates
(contact)
Nasopha
Sample type
Tracheal
patient)
Sputum
ryngeal
swab
(in-
is in sequence.
Method
Test
Category
Pathogen
Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
Broncho- 1 -3 Sterile 5 days Daily 1329/ √ √ √ √
at 2°C – 8°C
after collection
Send within 24 hours
MKAK-BPU-U01
RM 70.00
Bacteriology
alveolar ML container 1330
lavage
(BAL) (in-
patient)
CSF 1 ML Sterile 5 days Daily 1329/ √ √ √ √ CSF which is
at 2°C – 8°C
after collection
Send within 1 hour
MKAK-BPU-U01
RM 70.00
Bacteriology
container 1330 received more
than 1 hour not
suitable for
culturing.
PCR CSF (in- 1 ML Sterile 2 days Daily 1329/ √ 1. Please refer to

at 2°C – 8°C
collection
MKAK-BPU-U01
RM 350.00
Bacteriology
patient) container 1330 Specimen
Collection
Procedures for
Disease Division
Laboratory
Testing.
2. Delay in
Serum 3 ML Plain Tube 2 days Daily 1329/ √ transportation will
at 2°C – 8°C
after collection
Send immediately
MKAK-BPU-U01
RM 350.00
Bacteriology
1330 affect the quality
of the sample.

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
Plasma 3 ML EDTA tube Daily 1329/ √
at 2°C – 8°C
after collection
Send immediately
MKAK-BPU-U01
RM 350.00
Bacteriology
1330
Full Bacterial Blood Agar 7 days Daily 1329/ √ Inoculate pure
Pure isolate
MKAK-BPU-U01
RM 91.81
Bacteriology
Serotypin Culture Send at 1330 single colony onto
g 2°C – Blood Agar.
8°C
Outbreak or Serum 3 ML Plain Tube 3 days Daily 1329/ √ √ √ √ 1. Please refer to

Treponema pallidum (Syphilis)
Flocculation Test
at 2°C – 8°C
MKAK-BPU-U01
RM 30.00
Bacteriology
Diagnostic or 1330 Specimen
PT EQA Collection
Procedures for
Disease Division
Laboratory
Testing.
2. Delay in
transportation will
affect the quality
of the sample.

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
Plasma 3 ML Plain Tube 3 days Daily 1329/ √ √ √ 3. Haemolysed
at 2°C – 8°C
MKAK-BPU-U01
RM 30.00
Bacteriology
1330 sample shall be
rejected.
TPPA Serum 3 ML Plain Tube 3 days Daily 1329/ √ √ √ 1. Please refer to

MKAK-BPU-U01
RM 30.00
Bacteriology
1330 Specimen
Collection
Procedures for
Disease Division
Laboratory
Testing.
2. Delay in
transportation will
affect the quality
of the sample.
Plasma 3 ML Plain Tube 3 days Daily 1329/ √ √ √
MKAK-BPU-U01
RM 30.00
Bacteriology
1330 3. Haemolysed
sample shall be
rejected.

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
Varicella Outbreak PCR Vesicular - Do not 2-8°C √ 1. Sample should
MKAK-BPU-U01
PCR - 5 days
PCR-Daily
PCR-RM210.20
Virology
Molecular Unit 1321

Zoster fluid place be collected within
(collect transport 5 days after onset
with medium of illness.
polyester into the
swab) tube; the 2. A brief concise
specimen history of illness
MUST be and physical
kept dry findings is required
especially the date
Maculop - Sterile
of onset of illness
apular screw
and date of sample
lesions capped
collection.
or crusts container
from
lesions
CSF 1-3 ml Sterile
screw
capped
container
Outbreak or Fresh 3-5 ml Sterile 5 days Daily 1329/ √ √ √ √ √ 1. Please refer to

Vibrio cholerae/ Vibrio sp

MKAK-BPU-U01
RM 70.00
Bacteriology
Diagnostic or stool container 1330 Specimen
Procedures for
Disease Division
Laboratory
Testing.
2 .A sample which
is received more
than 2 hours will
be rejected.

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media

MKAK-BPU-U01
RM 70.00
Bacteriology
stool 1330 Specimen
Collection
Procedures for
Disease Division
Laboratory
Testing.
2. Delay in
transportation will
of the bacteria.

MKAK-BPU-U01
RM 70.00
Bacteriology
swab 1330 Specimen
Collection
Procedures for
Disease Division
Laboratory
Testing.
2. Ensure the swab

shows some faecal
staining to avoid
low quality of
sampling.
Serotypin Bacterial NA slant Send at 7 days Daily 1329/ √ √ √ √ √
MKAK-BPU-U01
RM 20.00
Bacteriology
g Culture 2°C – 1330 3. Delay in
Pure isolate
8°C transportation will

of the bacteria.

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
Bacterial NA slant Send at 30 days 1329/ √ √
Electrophoresis
Pulsed-field Gel
Pure isolate
MKAK-BPU-U01
Requirement
Special
RM 350.00
Bacteriology
Culture 2°C – 1330
8°C
West Nile Outbreak qPCR Serum 1-3 ml Plain tube 2-8°C √ 1. Sample should
qPCR - 3 days
qPCR- Daily
qPCR-RM257.00
Virology
Molecular Unit 1321

virus with serum be collected within
of illness.
CSF 1 ml Sterile
especially the date
of onset of illness
and date of sample
collection.
Yellow Outbreak qPCR Serum 1-3 ml Plain tube 2-8°C √ 1. Sample should
qPCR - 3 days
qPCR- Daily
qPCR-RM257.00
Virology
Molecular Unit 1321

fever virus with serum be collected within
of illness.
CSF 1 ml Sterile
especially the date
of onset of illness
and date of sample
collection.

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
Outbreak or Culture & Fresh 3-5 Sterile 5 days Daily 1329/ √ √ √ √ √ 1. Please refer to
Yersinia enterocolitica
at 2°C – 8°C
MKAK-BPU-U01
RM 70.00
Bacteriology
Diagnostic Sensitivit stool gm container 1330 Specimen
y Collection
Procedures for
Disease Division
Laboratory
Testing.
5 days Daily 1329/ √ √ √ √ √ 2. A sample which
MKAK-BPU-U01
RM 70.00
Bacteriology
1330 is received more
than 2 hours will
be rejected.
temperature. If delay is unavoidable, send at 2°C – 8°C

MKAK-BPU-U01
RM 70.00
Bacteriology
stool 1330 Specimen
Collection
Procedures for
Disease Division
Laboratory
Testing.
2. Delay in
transportation will
of the bacteria.

Public Health
Sample Volume
Transportation
Cost/test (RM)
Frequency of
Laboratories
Sample type
is in sequence.
Laboratory
Tel No. 03-

61261200
Container/
Sample
Ext. No
name
Test Type of
test
Johor Bahru
K. Kinabalu
Kota Bharu
MKA Ipoh
Method form
MKAK
MKA
MKA
MKA
Media
temperature. If delay is unavoidable, send at 2°C – 8°C

MKAK-BPU-U01
RM 70.00
Bacteriology
swab 1330 Specimen
Collection
Procedures for
Disease Division
Laboratory
Testing.
2. Ensure the swab

shows some faecal
staining to avoid
low quality of
sampling.
3. Delay in
transportation will
of the bacteria.
Zika virus Outbreak qPCR Serum 1-3 ml Plain tube 2-8°C √ 1. Sample should
qPCR - 3 days
qPCR- Daily
qPCR-RM257.01
Virology
Molecular Unit 1321

with serum be collected within
of illness.
CSF 1 ml Sterile
2. A brief concise
screw
history of illness
capped
and physical
especially the date
of onset of illness
and date of sample
collection.

B. The Comprehensive List of Biochemistry Tests in NPHL
IMPORTANT POINT TO NOTE:
The Cost Per Tests listed in this directory are NOT the actual payment charges for the tests. The costs included in this directory are for user reference and
understanding/appreciation of the costs involved to run tests which can be very expensive. For actual payment charges per tests, please refer to the PERINTAH FI
(PERUBATAN) (KOS PERKHIDMATAN) pua_20141231_P.U. (A) 363.
Time (LTAT) working days

Laboratory Turn-Around-
Sample Transportation
Tel No:03-61261200
Laboratory Name
Transport media
PHL
Sample Volume
Cost/ Test (RM)

Container/
Category
Ext. No.
Sample Type of
Profile Analyte Test Method Reference Range Remarks
type form
MKA Johor Bahru
MKA Kota Bharu
MKA K. Kinabalu
MKA Ipoh
MKAK
RBC Direct Current Whole 3 ml EDTA 4 - 8 0C MKAK- 3 3.5 - 5.5 x 106 / uL √ Results can
Surveillance
RM15
BIOCHEMISTRY
1287
(DC) detection blood tube BPU-U01 be
method obtained
manually
or by post
WBC DC detection Whole 3 ml EDTA 4 - 8 0C MKAK- 3 4.0 - 10.0 x 103 / uL √
method blood tube BPU-U01
PLT Direct Current Whole 3 ml EDTA 4 - 8 0C MKAK- 3 150 - 450 103 / uL √
(DC) detection blood tube BPU-U01
method
Full blood HGB Non-cyanide Whole 3 ml EDTA 4 - 8 0C MKAK- 3 11.0 - 16.0 g / dL √

count method blood tube BPU-U01
HCT Cumulative Whole 3 ml EDTA 4 - 8 0C MKAK- 3 37.0 - 54.0 % √
pulse height blood tube BPU-U01
detection
method
LYM%, Automatic Whole 3 ml EDTA 4 - 8 0C MKAK- 3 20-40 % √

floating blood tube BPU-U01
discriminators
Page 135
Tel No:03-61261200
Laboratory Name
Transport media
PHL
Sample Volume
Cost/ Test (RM)

Container/
Category
Ext. No.
Sample Type of
type form
MKA Johor Bahru
MKA Kota Bharu
MKA K. Kinabalu
MKA Ipoh
MKAK
MXD%, Automatic Whole 3 ml EDTA 4 - 8 0C MKAK- 3 3.0-15 % √
discriminators
NEUT% Automatic Whole 3 ml EDTA 4 - 8 0C MKAK- 3 50-70 % √
discriminators
Mercury AAS 20 ml 4 - 8 0C MKAK- 7 <10 ug/L √
Surveillance
Random urine
container
Urine
HEAVY METAL
1333
Appointment by requestor.
RM 60
BPU-U01
Cadmium GFASS Whole 3 ml 4 - 8 0C MKAK- 7 < 10.0 ug/l √
RM 60
EDTA / EDTA tube
Heavy blood Trace element BPU-U02
Metal
Chromium GFASS 20 ml 4 - 8 0C MKAK- 7 < 0.25 µg/L √

Random urine
RM60
BPU-U03
container
Urine
B. The Comprehensive List of Biochemistry Tests in NPHL Page 136

Tel No:03-61261200
Laboratory Name
Transport media
PHL
Sample Volume
Cost/ Test (RM)

Container/
Category
Ext. No.
Sample Type of
type form
MKA Johor Bahru
MKA Kota Bharu
MKA K. Kinabalu
MKA Ipoh
MKAK
Lead GFASS Whole 3 ml 4 - 8 0C MKAK- 7 <10ug/dl √
RM 60
EDTA / EDTA tube
blood BPU-U04
Trace element
Lipid Cholesterol Cholesterol Serum/ 5 ml 4 - 8 0C MKAK- 3 4.0 - 5.9 mmol/L √ √ √ √ For Fasting
Surveillance
Heparin
Plain/
RM75
BIOCHEMISTRY
1287
Profile Oxidase plasma BPU-U01 Lipid
Profile,
blood
should be
Hdl- Immunological Serum/ 5 ml 4 - 8 0C MKAK- 3 < 1.03 Low HDL √ √ √ √
Heparin
Plain/
collected
Cholesterol inhibition plasma BPU-U01 ≥1.55 High HDL after an
overnight
fasting for
Triglyceride Lipase/GPO-PAP Serum/ 5ml 4 - 8 0C MKAK- 3 0.40 - 1.71 mmol/L √ √ √ √ at least 8
Plain/ Heparin
colorimetric plasma BPU-U01 hours with

without glycerol no intake
correction of food or
drinks
except
water
Albumin Bromocresol Serum/ 5 ml 4 - 8 0C MKAK- 3 35 - 52 g/L √ √ √ √ Results can
Plain/Heparin
RM 75
BIOCHEM
1287
Liver
Surveillance
green (BCG) plasma BPU-U01 be

Function
obtained
Test
manually
or by post

Tel No:03-61261200
Laboratory Name
Transport media
PHL
Sample Volume
Cost/ Test (RM)

Container/
Category
Ext. No.
Sample Type of
type form
MKA Johor Bahru
MKA Kota Bharu
MKA K. Kinabalu
MKA Ipoh
MKAK
ALP AMP, Optimised Serum/ 5 ml 4 - 8 0C MKAK- 3 30 - 120 U/L √ √ √ √
Heparin
to IFCC plasma BPU-U01
Plain/
ALT Rate UV, Tris Serum/ 5 ml 4 - 8 0C MKAK- 3 ♀ - 0-35 U/L √ √ √ √
Plain/ Heparin
buffer without plasma BPU-U01 ♂ - 0-50 U/L
Pyridoxal
phosphate,
IFCC/SFBC
AST Rate UV, Tris Serum/ 5 ml 4 - 8 0C MKAK- 3 ♀ - 0-35 U/L √ √ √ √
Plain/ Heparin
buffer without plasma BPU-U01 ♂ - 0-50 U/L
Pyridoxal
phosphate,
IFCC/SFBC
Total Dichlorophenyl Serum/ 5 ml 4 - 8 0C MKAK- 3 5.0 - 21.0 umol/L √ √ √ √

Heparin
Plain/
Bilirubin Diazonium plasma BPU-U01
Total Biuret reaction, Serum/ 5 ml 4 - 8 0C MKAK- 3 66 - 83 g/L √ √ √ √

Heparin
Plain/
Protein end point plasma BPU-U01

Tel No:03-61261200
Laboratory Name
Transport media
PHL
Sample Volume
Cost/ Test (RM)

Container/
Category
Ext. No.
Sample Type of
type form
MKA Johor Bahru
MKA Kota Bharu
MKA K. Kinabalu
MKA Ipoh
MKAK
Calcium Cresolphthalein Serum/ 5 ml 4 - 8 0C MKAK- 3 2.20 - 2.65 mmol/L RM30 √ √ √ √ Results can
Miscellaneous Tests
Surveillance
Heparin
Plain/
BIOCHEMISTRY
1287
complexone plasma BPU-U01 be
obtained
manually
or by post
Fasting Hexokinase Plasma 3 ml 4 - 8 0C MKAK- 3 4.1 – 5.9 mmol/L RM30 √ √ √ √ For Fasting
Fluoride tube
Glucose BPU-U01 Glucose,
blood
should be
collected
after an
overnight
fasting for
at least 8
hours with
no intake
of food or
drinks
except
water
Inorganic Phosphomolyb- Serum/ 5 ml 4 - 8 0C MKAK- 3 0.81 - 1.45 mmol/L √ √ √ √ Results can
Heparin
Plain/
phosphate date formation plasma BPU-U01 RM30 be

obtained
manually
or by post

Urinary Ammonium Urine 10 ml MKAK- 25 School-age children √ √ √ √ Results can
Nutritional related diseases
Surveillance
Urine container
-20oC (> 7 days)

4 - 8 0C (< 7 days)
RM 60
BIOCHEMISTRY
1287
Iodine Persulfate BPU-U01 (6 years or older) be
Digestion with <20 Insufficient - obtained
Spectrophotom Severe iodine by post
etric Detection deficiency
of the Sandell- 20–49 Insufficient -
Kolthoff Moderate iodine
Reaction, deficiency
Modified 50–99 Insufficient -
Microplate Mild iodine
Method deficiency
100–199 Adequate
- Adequate iodine
nutrition
200–299 Above
requirements - May
pose a slight risk of
more than adequate
iodine intake in
these populations
≥300 Excessive -
Risk of adverse
health
consequences
(iodine-induced
hyperthyroidism,
autoimmune
thyroid disease)
Pregnant women
<150 Insufficient
150–249 Adequate
250–499 Above
requirements
≥500 Excessive
Lactating mother
<100 Insufficient
≥100 Adequate

Tel No:03-61261200
Laboratory Name
Transport media
PHL
Sample Volume
Cost/ Test (RM)

Container/
Category
Ext. No.
Sample Type of
type form
MKA Johor Bahru
MKA Kota Bharu
MKA K. Kinabalu
MKA Ipoh
MKAK
Renal Chloride ISE indirect Serum/ 5 ml 4 - 8 0C MKAK- 3 101 - 109 mmol/L √ √ √ √ Results can
Surveillance
BIOCHEMISTRY
1287
RM 75
Heparin
Profile plasma BPU-U01 be
Plain/
obtained
manually
or by post
Creatinine Alkaline picrate Serum/ 5 ml 4 - 8 0C MKAK- 3 ♀ - 58 - 96 mmol/L √ √ √ √
Heparin
plasma BPU-U01 ♂ - 74 - 110 mmol/L
Plain/
Potassium ISE indirect Serum/ 5 ml 4 - 8 0C MKAK- 3 3.5 - 5.1 mmol/L √ √ √ √
Heparin
plasma BPU-U01
Sodium ISE indirect Serum/ 5 ml Plain/ 4 - 8 0C MKAK- 3 136 - 146 mmol/L √ √ √ √

Heparin
plasma BPU-U01
Plain/
Urea Urease kinetic Serum/ 5 ml 4 - 8 0C MKAK- 3 2.8 - 7.2 mmol/L √ √ √ √

Heparin
plasma BPU-U01
Plain/
Uric Acid Uricase Serum/ 5 ml 4 - 8 0C MKAK- 3 ♀ -154.7 - 357.0 √ √ √ √

plasma BPU-U01 mmol/L
Heparin
Plain/
♂ -208.3 – 428.4
mmol/L

Tel No:03-61261200
Laboratory Name
Transport media
PHL
Sample Volume
Cost/ Test (RM)

Container/
Category
Ext. No.
Sample Type of
type form
MKA Johor Bahru
MKA Kota Bharu
MKA K. Kinabalu
MKA Ipoh
MKAK
Thyroid Thyroid Immunochemi- Serum 5 ml Plain 4 - 8 0C MKAK- 2 01 – 23 months √ √ √ √ Results can
Surveillance
BIOCHEMISTRY
1287
RM 60
Function Stimulating luminometric / Cord tube BPU-U01 (Infants)* 0.87 – be
Test Hormone (ICMA) Blood 6.15* obtained
2 to < 12 (Pediatrics) manually
0.64 - 6.27 or by post
12 to < 18
(Adolescents)
0.51 - 4.94
≥18 (Adults)
0.55 - 4.78
Free T4 Luminescent Serum 5 ml Plain 4 - 8 0C MKAK- 2 Infants (01 – 23 √ √ √ √
Immunoassay / Cord tube BPU-U01 months)* 12.1 –
(LIA) Blood 18.6*
Eurothyroid
11.5 - 22.7
Hypothyroid
less than 11.5
Hyperthroid
greater than 22.7
Tobacco Carbon ISO 8454 Maximum Allowable √ For
Surveillance
Cigarettes
200 sticks / brand / lot
(unopened box)
Original packaging,
Room Temperature
Sampel Hasil Tembakau

Permintaan Bagi Analisis
Borang 2 (Peraturan 19)
- 25
Screening - 14 Confirmation
RM 350
TAR & NIKOTIN
1268
Control Monoxide Level for Nicotine: monitoring
1.0 mg/cig program
under
Control of
Tobacco
Product
Regulations
2004, Food
Act 1983.

Page 143
enforceme
surveillanc
Remarks
obtained
This may
could be
by post
include
Result
e and
nt.
MKA K. Kinabalu
√
MKA Kota Bharu

PHL
MKA Johor Bahru

√
MKA Ipoh
√
MKAK
Tel No:03-61261200 1287
Ext. No.
Laboratory Name BIOCHEMISTRY
Cost/ Test (RM)
RM 30
Maximum Allowable
Maximum Allowable
♀ - 3.93 - 10.8 kUl/L

♂ - 4.62 - 11.5 kUl/L
Reference Range
Level for Tar: 10
Level for COr: 10

mg/cig
mg/cig
Laboratory Turn-Around- Screening - 14 Screening - 14

7
Time (LTAT) working days Confirmation - 25 Confirmation - 25

Type of
form
MKAK/BKM/Cholin-01
Room Temperature 4 - 8 0C
Sample Transportation Room Temperature
Container/ Original packaging, Original packaging,
Plain
tube
Transport media (unopened box) (unopened box)
200 sticks / brand / lot 200 sticks / brand / lot
5 ml
Sample Volume
Sample
Cigarettes
Serum
type
Cigarettes
B. The Comprehensive List of Biochemistry Tests in NPHL

Test Method
GSCC 1994
ISO 10315
ISO 4387
Category Surveillance
Cholinester
Nicotine
Analyte
ase
Tar
Toxicology
Substance
Profile
Abuse
and
C. The Comprehensive List of Food Division Tests in NPHL
IMPORTANT POINTS TO NOTE:

Details of Food testing services provided by the other Public Health Laboratories and Food Safety and Quality Laboratories under MOH can be found at
fsq.moh.gov.my/v5/ms/category/perkhidmatan/
1. Microbiology
Sample condition
MRL
LOD
Unit Microorganism Food Matrix LOQ (Unit) (Reference)/Guideline
(Unit) Perishable /
Sample (Reference)
Non- Temperature Packaging Other Information
weight
perishable
Reference sample
1.0 X 10E2
Food NA Perishable 250 g must be included in
CFU/g 0.0˚C to 4.4˚C
the ice box
Sterile
1.0 X 10E2 packaging/ Sample in frozen
Frozen Samples NA Perishable 250 g Absent (regulation 39)
CFU/g -18˚C to 0˚C Original condition
FOOD MICROBIOLOGY
Container
Samples of shelf
Not exceeding Protected from direct
stable packaged 1.0 X 10E2 Non-
NA 250 g ambient sunlight or from other
Bacillus cereus products & dried CFU/g perishable
temperature sources of heat
products
Sterile
Reference sample
1.0 X 10E1 packaging/
Water NA Perishable 250 g must be included in Absent (regulation 39)
CFU/ml 0.0˚C to 4.4˚C Original
the ice box
Container
Original Reference sample

Swab Samples NA NA Perishable 1 unit 1˚C to 4˚C Container/ must be included in NA
Bottle the ice box
Page 144
Sample condition
MRL
LOD
(Unit) Perishable /
Sample (Reference)
weight
perishable
Sterile
Reference sample
0.04 packaging/
Brucella spp. Milk NA Perishable 250 g must be included in Absent (regulation 39)
CFU/g 0.0˚C to 4.4˚C Original
the ice box
Container
Reference sample
0.04
the ice box
Sterile
0.04 packaging/ Sample in frozen
Campylobacter spp. Frozen Samples NA Perishable 250 g Absent (regulation 39)
Container
Samples of shelf
stable packaged 0.04 Non-
products & dried CFU/g perishable
products
Reference samples
1.0 X 10E2
the ice box
Sterile
Frozen Samples NA Perishable 250 g
Container
Clostridium perfringens Samples of shelf Absent (regulation 39)
250 g or Not exceeding Protected from direct
NA min 3 ambient sunlight or from other
packs temperature sources of heat
products
Sterile
Reference sample
Water NA Perishable 250 g must be included in
the ice box
Container
C. The Comprehensive List of Food Division Tests in NPHL (Microbiology) Page 145
Sample condition
MRL
LOD
(Unit) Perishable /
Sample (Reference)
weight
perishable
Reference sample
Food Perishable 250 g must be included in
0.0˚C to 4.4˚C
the ice box
Sterile
Coagulase positive 1.0 X 10E2 packaging/ Sample in frozen
Frozen Samples Perishable 250 g Absent (regulation 39)
Staphylococci CFU/g -18˚C to 0˚C Original condition
Container
Samples of shelf
stable packaged Non-
products & dried perishable
products
Sterile
Reference sample
Water Perishable 250 g must be included in Absent (regulation 39)
Coagulase positive the ice box
Container
Staphylococci*
Swab Samples NA Perishable 1 unit Container/ must be included in NA
1˚C to 4˚C
Bottle the ice box
Reference sample
0.3 MPN/g
(food) 0.0˚C to 4.4˚C
the ice box
Sterile
0.3 MPN/g packaging/ Sample in frozen Fifteenth schedule
Coliform Frozen Samples NA Perishable 250 g
(food) -18˚C to 0˚C Original condition (regulation 39)
Container
Samples of shelf
stable packaged 1.0 X Non-
250 g ambient sunlight or from other
products & dried 10E1CFU/g perishable
products
Sample condition
MRL
LOD
(Unit) Perishable /
Sample (Reference)
weight
perishable

1.0 X
Swab Samples Perishable 1 unit Container/ must be included in NA
10E1CFU/g 1˚C to 4˚C
Bottle the ice box

1˚C to 4˚C
Bottle the ice box
Coliform: not more than
4 colonies per 100 mL,
E. coli: not listed
(Packaged drinking
water and vended
Sterile
Reference sample water-25th Schedule,
packaging/
Water/ Ice 1CFU/100ml Perishable 250 g must be included in sub regulation 360B (3)
0.0˚C to 4.4˚C Original
the ice box and 360C (3)) (Water-
Container
25A schedule, sub
regulation 394(1))
(Natural mineral water-
26 schedule, sub
regulation 360A (7)
Reference samples
0.04
the ice box
Sterile
Cronobacter sakazakii Frozen Samples NA Perishable 250 g Absent (regulation 39)
Container
Samples of shelf
products
Sample condition
MRL
LOD
(Unit) Perishable /
Sample (Reference)
weight
perishable
Reference sample
0.3 MPN/g
(food) 0.0˚C to 4.4˚C
the ice box
Sterile
0.3 MPN/g packaging/ Sample in frozen
Frozen Samples Perishable 250 g
(food) -18˚C to 0˚C Original condition
Container
Samples of shelf
products
Reference sample
0.3 MPN/g Fifteenth schedule
(food) 0.0˚C to 4.4˚C (regulation 39)
the ice box
Escherichia coli NA
Sterile
0.3 MPN/g packaging/ Sample in frozen
Frozen Samples Perishable 250 g
(food) -18˚C to 0˚C Original condition
Container
Samples of shelf
products
1.0 X
Swab Samples Perishable 1 unit Container/ must be included in
10E1CFU/g 1˚C to 4˚C
Bottle the ice box

1˚C to 4˚C
Bottle the ice box
Sample condition
MRL
LOD
(Unit) Perishable /
Sample (Reference)
weight
perishable
Coliform: not more than
4 colonies per 100 mL,
E. coli: not listed
(Packaged drinking
water and vended
Sterile
Reference sample water-25th Schedule,
packaging/
Water/ Ice 1CFU/100mL Perishable 250 g must be included in sub regulation 360B (3)
the ice box and 360C (3)) (Water-
Container
25A schedule,
subregulation 394(1))
(Natural mineral water-
26 schedule, sub
regulation 360A (7)
Reference samples
0.04
the ice box
Sterile
Escherichia coli O157 Frozen Samples NA Perishable 250 g Absent (regulation 39)
Container
Samples of shelf
products
Reference samples
0.04
Food NA Perishable 250 g Sterile must be included in
packaging/ the ice box
Escherichia coli 0157 Absent (regulation 39)
Original
0.04 Container Sample in frozen
CFU/g -18˚C to 0˚C condition
Sample condition
MRL
LOD
(Unit) Perishable /
Sample (Reference)
weight
perishable
Samples of shelf
products
Swab Samples NA NA Perishable 1 unit Container/ must be included in NA
1˚C to 4˚C
Bottle the ice box
Reference samples
0.04
the ice box
Sterile
Listeria 0.04 packaging/ Sample in frozen
monocytogenes CFU/g -18˚C to 0˚C Original condition
Container
Samples of shelf
products
Reference samples
1.0 X 10E2
the ice box
Sterile
Listeria 1.0 X 10E2 packaging/ Sample in frozen
monocytogenes CFU/g -18˚C to 0˚C Original condition
Container
Samples of shelf
250g or Not exceeding Protected from direct
products
Sample condition
MRL
LOD
(Unit) Perishable /
Sample (Reference)
weight
perishable
NIL in 100mL
(Packaged drinking
Sterile water and vended
Reference sample
Pseudomonas packaging/ water-25th Schedule,
Water NA 1CFU/100ml Perishable 250 g must be included in
aeruginosa 0.0˚C to 4.4˚C Original sub regulation 360B (3)
the ice box
Container and 360C (3)) (Water-
25A schedule,
subregulation 394(1))
Reference sample
0.04
the ice box
Sterile
Container
Samples of shelf Absent (regulation 39)
Salmonella spp. NA 250 g ambient sunlight or from other
products
Sterile
Reference sample
0.04 packaging/
the ice box
Container
1˚C to 4˚C
Bottle the ice box
Reference samples
1.0 X 10E1 Sterile
Staphylococcus aureus Food NA Perishable 250 g must be included in Absent (regulation 39)
CFU/g 0.0˚C to 4.4˚C packaging/
the ice box
Sample condition
MRL
LOD
(Unit) Perishable /
Sample (Reference)
weight
perishable
Original
1.0 X 10E1 Container Sample in frozen
Samples of shelf
products
Original Reference samples
1.0 X 10E1
CFU/g 1˚C to 4˚C
Bottle the ice box
Reference sample
1.0 X 10E1
the ice box
Sterile
1.0 X 10E1 packaging/ Sample in frozen Fifteenth schedule
CFU/g -18˚C to 0˚C Original condition (regulation 39)
Container
Samples of shelf
Total plate count NA min 3 ambient sunlight or from other
products
1.0 X 10E1
CFU/g 1˚C to 4˚C
Bottle the ice box
Sterile
Reference sample
packaging/
Water NA 1.0 CFU/ml Perishable 250 g must be included in NA
the ice box
Container
Sample condition
MRL
LOD
(Unit) Perishable /
Sample (Reference)
weight
perishable
Sterile
Reference sample
1.0 X 10E2 packaging/ Fifteenth schedule
CFU/g 0.0˚C to 4.4˚C Original (regulation 39)
the ice box
Container
Reference sample
0.04
the ice box
Sterile
Container
products
Sterile
Reference sample
Vibrio cholerae 0.04 packaging/
the ice box
Container
1˚C to 4˚C
Bottle the ice box
Reference sample
0.04
Food NA Perishable 250 g Sterile must be included in
packaging/ the ice box
Absent (regulation 39)
Original
0.04 Container Sample in frozen
Sample condition
MRL
LOD
(Unit) Perishable /
Sample (Reference)
weight
perishable
Samples of shelf
products
Sterile
Reference sample
0.04 packaging/
the ice box
Container
1˚C to 4˚C
Bottle the ice box
Reference sample
0.04
the ice box
Sterile
Container
Vibrio stable packaged 0.04 Non-
parahaemolyticus products & dried CFU/g perishable
products
Sterile
Reference sample
0.04 packaging/
the ice box
Container
Sterile
Reference sample
0.04 packaging/
Food NA Perishable 250 g must be included in NA
the ice box
Container
Sample condition
MRL
LOD
(Unit) Perishable /
Sample (Reference)
weight
perishable
Reference samples
1.0 X 10E1
the ice box
Sterile
Yeast and Mold Count Frozen Samples NA Perishable 250 g Not listed
Samples of shelf Container
products
Note: * No SAMM Accreditation
2. Molecular
Sample condition
LOQ (Unit)
Perishable / MRL (Reference)/
Unit Microorganism Food Matrix LOD (Unit) Sample
Non- Temperature Packaging Other Information Guideline (Reference)
weight
perishable
Sterile
Reference sample
Milk and milk packaging/
Brucella 1.0 x 10E-3 ng/µl NA Perishable 250g 0.0˚C to 4.4˚C must be included in NA
products Original
the ice box
Container
Molekular Makanan
Samples of fresh,
perishable,
Sterile
refrigerated, food
packaging/
poisoning products, Perishable 250g 0.0˚C to 4.4˚C
Original
semi-preserved
Container
products, RTE, water
and ice
Sterile
Reference sample
packaging/
Campylobacter Frozen Samples NA NA Perishable 250g must be included in NA
-18˚C to 0˚C Original
the ice box
Container
Samples of shelf Sterile
250g or Not exceeding
stable packaged Non- packaging/
min 3 ambient
products & dried perishable Original
packs temperature
products Container
Original
Swab Samples Perishable 1 unit Container/
1˚C to 4.4˚C
Bottle
Page 156
Sample condition
LOQ (Unit)
weight
perishable
Samples of fresh,
perishable,
Sterile
refrigerated, food
packaging/
Original
semi-preserved
Container
and ice
Sterile
packaging/
Frozen Samples Perishable 250g Reference sample
Cronobacter -18˚C to 0˚C Original
NA NA must be included in NA
sakazakii Container
the ice box
min 3 ambient
packs temperature
products Container
Original
1˚C to 4.4˚C
Bottle
Samples of fresh,
perishable,
Sterile
refrigerated, food
packaging/
Original
semi-preserved Reference sample
Container
Escherichia coli products, RTE, water NA NA must be included in NA
and ice the ice box
Sterile
packaging/
Frozen Samples Perishable 250g
Container
C. The Comprehensive List of Food Division Tests in NPHL (Molecular) Page 157
Sample condition
LOQ (Unit)
weight
perishable
min 3 ambient
packs temperature
products Container
Original
1˚C to 4.4˚C
Bottle
Samples of fresh,
perishable,
Sterile
refrigerated, food
packaging/
Original
semi-preserved
Container
and ice
Sterile Reference sample
NA NA packaging/ must be included in NA
-18˚C to 0˚C Original the ice box
Container
min 3 ambient
packs temperature
products Container
Original
1˚C to 4.4˚C
Bottle
Samples of fresh,
perishable,
Sterile
refrigerated, food Reference sample
packaging/
poisoning products, NA NA Perishable 250g 0.0˚C to 4.4˚C must be included in NA
Original
semi-preserved the ice box
Container
and ice
Sample condition
LOQ (Unit)
weight
perishable
Sterile
packaging/
Container
min 3 ambient
packs temperature
products Container
Original
1˚C to 4.4˚C
Bottle
Samples of fresh,
perishable,
Sterile
refrigerated, food
packaging/
Original
semi-preserved
Container
and ice
NA Sterile Reference samples
Listeria 0.04 CFU/g packaging/ must be included in NA
-18˚C to 0˚C Original the ice box
Container
min 3 ambient
packs temperature
products Container
Original
Swab Samples NA Perishable 1 unit Container/
1˚C to 4.4˚C
Bottle
Sample condition
LOQ (Unit)
weight
perishable
Samples of fresh,
perishable,
Sterile
refrigerated, food Reference samples
packaging/
Salmonella poisoning products, NA Perishable 250g 0.0˚C to 4.4˚C must be included in
Original
Container
and ice
Sterile
0.04CFU/g packaging/ NA
Container
min 3 ambient
packs temperature
products Container
Original
1˚C to 4.4˚C
Bottle
Samples of fresh,
perishable,
Sterile
packaging/
poisoning products, 1.0 x 10E-4 ng/µl NA Perishable 250g 0.0˚C to 4.4˚C must be included in NA
Original
Container
and ice
Sterile
packaging/
Container
min 3 ambient
packs temperature
products Container
Sample condition
LOQ (Unit)
weight
perishable
Original
1˚C to 4.4˚C
Bottle
Samples of fresh,
perishable,
Sterile
packaging/
poisoning products, 1.0 x 10E-3 ng/µl NA Perishable 250g 0.0˚C to 4.4˚C must be included in NA
Original
Container
and ice
Sterile
packaging/
Container
min 3 ambient
packs temperature
products Container
Original
1˚C to 4.4˚C
Bottle
Samples of fresh,
perishable,
Sterile
refrigerated, food
packaging/
poisoning products, NA Perishable 250g 0.0˚C to 4.4˚C
Original
semi-preserved Reference samples
Staphylococcus Container
products, RTE, water NA must be included in NA
aureus
and ice the ice box
Sterile
packaging/
Frozen Samples NA Perishable 250g
Container
Sample condition
LOQ (Unit)
weight
perishable
NA min 3 ambient
packs temperature
products Container
Original
Swab Samples NA Perishable 1 unit Container/
1˚C to 4.4˚C
Bottle
Samples of fresh,
perishable,
Sterile
packaging/
poisoning products, NA Perishable 250g 0.0˚C to 4.4˚C must be included in NA
Original
Container
and ice
Vibrio cholerae Sterile
0.5 ng/µl packaging/
Container
min 3 ambient
packs temperature
products Container
Original
1˚C to 4.4˚C
Bottle
Samples of fresh,
perishable,
Sterile
Vibrio packaging/
poisoning products, NA NA Perishable 250g 0.0˚C to 4.4˚C must be included in NA
parahaemolyticus Original
Container
and ice
Sample condition
LOQ (Unit)
weight
perishable
Sterile
packaging/
Container
min 3 ambient
packs temperature
products Container
Original
1˚C to 4.4˚C
Bottle
Samples of fresh,
perishable,
Sterile
refrigerated, food
packaging/
Original
semi-preserved
Container
and ice
Sterile
packaging/
-18˚C to 0˚C Original Reference samples
NA NA Container must be included in NA
the ice box
min 3 ambient
packs temperature
products Container
Original
1˚C to 4.4˚C
Bottle
Sample condition
LOQ (Unit)
weight
perishable
Samples of fresh,
perishable,
Sterile
packaging/
poisoning products, Perishable 250g 0.0˚C to 4.4˚C must be included in
Original
Container
and ice
Sterile
Reference samples
packaging/
Frozen Samples Perishable 250g must be included in
Yersinia -18˚C to 0˚C Original
NA NA the ice box NA
enterocolitica Container

250g or Not exceeding Reference samples
min 3 ambient must be included in
packs temperature the ice box
products Container
Original Reference samples

Swab Samples Perishable 1 unit Container/ must be included in
1˚C to 4.4˚C
Bottle the ice box
VIRUS
Sterile
Reference samples
packaging/
Adenovirus Water 6.0x10E-7 ng/µl NA Perishable 250g 0.0˚C to 4.4˚C must be included in
Original
the ice box
Container
Sample condition
LOQ (Unit)
weight
perishable
Bivalve Molluscans 250g &

Sterile
Shellfish (BMS), fresh 300g Reference samples
packaging/
Hepatitis A Virus vegetables (salad or 100 RT-PCR unit/µl NA Perishable (Bivalve 0.0˚C to 4.4˚C must be included in NA
Original
slam), soft fruits Molluscan the ice box
Container
(berries) and water s Shellfish)
Bivalve Molluscans 250g &

Sterile
Shellfish (BMS), fresh 300g Reference samples
1.0 x 10E2 packaging/
Rotavirus vegetables (salad or NA Perishable (Bivalve 0.0˚C to 4.4˚C must be included in
copies/µl Original
slam), soft fruits Molluscan the ice box
Container
(berries) and water s Shellfish)
3. GMO
Sample condition
LOD LOQ MRL (Reference)/Guideline
Unit Parameter Food Matrix Perishable /
(Unit) (Unit) Other (Reference)
Non- Sample weight Temperature Packaging
Information
perishable
Raw - min 1 kg
Fruit, Products - 2
Agrobacterium Perishable / Peraturan 11 Part IV
vegetable packs of Frozen/Chilled/Room Any suitable
tumefaciens no’s 3’ 0.1% NA Non- None Peraturan -Peraturan
and their commercial Temperature packaging
Terminator gene perishable Makanan 1985
products packaging; min
200 gm/pack
Raw - min 500

gm (edible
portion without
Animal
Perishable / bones) Peraturan 11 Part IV
tissue and Frozen/Chilled/Room Any suitable
Bovine DNA NA NA Non- products - 2 None Peraturan -Peraturan
GMO food Temperature packaging
perishable packs of Makanan 1985
products
commercial
packaging; min
200 gm/pack
Raw - min 500

gm (edible
portion without
Animal
* Bovine DNA NA NA Non- products - 2 None Peraturan -Peraturan
food Temperature packaging
products
commercial
packaging; min
200 gm/pack
Page 166
Sample condition
Information
perishable
Raw - min 500

gm (edible
portion without
Animal
* Bubalus bubalis DNA NA NA Non- products - 2 None Peraturan -Peraturan
products
commercial
packaging; min
200 gm/pack
Raw - min 500
gm (edible
portion without
Animal
* Bubalus bubalis DNA NA NA Non- products - 2 None Peraturan -Peraturan
products
commercial
packaging; min
200 gm/pack
Raw - min 1 kg
Fruit, Products - 2
Perishable / Peraturan 11 Part IV
CaMV 35S Promoter vegetable packs of Frozen/Chilled/Room Any suitable
0.1% NA Non- None Peraturan -Peraturan
gene and their commercial Temperature packaging
perishable Makanan 1985
200 gm/pack
Raw - min 1 kg
Products - 2
Animal and Perishable /
packs of Frozen/Chilled/Room Any suitable
DNA Product vegetable NA NA Non- None NA
commercial Temperature packaging
fats perishable
packaging; min
200 gm/pack
C. The Comprehensive List of Food Division Tests in NPHL (GMO) Page 167
Sample condition
Information
perishable
Raw - min 1 kg
Products - 2
All food Perishable /
Extraction all food packs of Frozen/Chilled/Room Any suitable
except for NA NA Non- None NA
type except oil commercial Temperature packaging
oil perishable
packaging; min
200 gm/pack
Raw - min 1 kg
Products - 2
Perishable /
Extraction Highly Liquid food packs of Frozen/Chilled/Room Any suitable
NA NA Non- None NA
processed Product - soya commercial Temperature packaging
perishable
packaging; min
200 gm/pack
Raw
material for
Extraction raw Chilled/Room Any suitable
soya, corn, NA NA Perishable Raw - min 1 kg None NA
material Temperature packaging
potato and
tomato
Raw - min 500

gm (edible
Raw and portion without
process bones)
Extraction Frozen/Chilled/Room Any suitable
product for NA NA Perishable products - 2 None NA
tissue/meat Temperature packaging
meat packs of
product commercial
packaging; min
200 gm/pack
Sample condition
Information
perishable
Raw - min 500

gm (edible
portion without
Animal
Gallus gallus tissue and 0.0355 Frozen/Chilled/Room Any suitable
NA Non- products - 2 None Peraturan -Peraturan
domesticus DNA food ng/ul Temperature packaging
products
commercial
packaging; min
200 gm/pack
Raw - min 1 kg
Soybean Products - 2
Kernels and 0.1 packs of Frozen/Chilled/Room Any suitable
Lectin gene NA Non- None Peraturan -Peraturan
soybean ng/ul commercial Temperature packaging
200 gm/pack
Raw - min 1 kg
Corn Products - 2
kernels and packs of Frozen/Chilled/Room Any suitable
Maize Bt 11 gene 0.1% NA Non- None Peraturan -Peraturan
Corn commercial Temperature packaging
Products packaging; min
200 gm/pack
Raw - min 1 kg
Corn Products - 2
Maize Bt 176 gene 0.1% NA Non- None Peraturan -Peraturan
200 gm/pack
Sample condition
Information
perishable
Raw - min 1 kg
Corn Products - 2
Maize GA 21 gene 0.1% NA Non- None Peraturan -Peraturan
200 gm/pack
Raw - min 1 kg
Corn Products - 2
kernels and 0.1 packs of Frozen/Chilled/Room Any suitable
Maize gene NA Non- None Peraturan -Peraturan
Corn ng/ul commercial Temperature packaging
200 gm/pack
Raw - min 1 kg
Corn Products - 2
Maize MON 810 gene 0.1% NA Non- None Peraturan -Peraturan
200 gm/pack
Raw - min 1 kg
Corn Products - 2
Maize T25 gene 0.1% NA Non- None Peraturan -Peraturan
200 gm/pack
Raw - min 1 kg
Kernels and packs of Frozen/Chilled/Room Any suitable
* MON 89788 NA NA Non- None Peraturan -Peraturan
soybean commercial Temperature packaging
200 gm/pack
Sample condition
Information
perishable
Raw - min 1 kg
Products - 2
Potato and Perishable / Peraturan 11 Part IV
* New Leaf Plus gene potato 0.1% NA Non- None Peraturan -Peraturan
products perishable Makanan 1985
packaging; min
200 gm/pack
Raw - min 1 kg
Products - 2
* New Leaf Y gene potato 0.1% NA Non- None Peraturan -Peraturan
packaging; min
200 gm/pack
Raw - min 1 kg
Fruit, Products - 2
vegetable 0.01 packs of Frozen/Chilled/Room Any suitable
* nptII gene NA Non- None Peraturan -Peraturan
and their ng/ul commercial Temperature packaging
200 gm/pack
Raw - min 1 kg
Products - 2
0.1 packs of Frozen/Chilled/Room Any suitable
Patatin gene potato NA Non- None Peraturan -Peraturan
ng/ul commercial Temperature packaging
packaging; min
200 gm/pack
Sample condition
Information
perishable
Raw - min 500

gm (edible
portion without
Fruit,
* Phosphinothricin N- Perishable / bones) Peraturan 11 Part IV
vegetable Frozen/Chilled/Room Any suitable
acetyltransferase (pat) NA NA Non- products - 2 None Peraturan -Peraturan
and their Temperature packaging
gene perishable packs of Makanan 1985
products
commercial
packaging; min
200 gm/pack
Raw - min 1 kg
Products - 2
DNA 0.001 packs of Frozen/Chilled/Room Any suitable
Plant gene NA Non- None Peraturan -Peraturan
Product ng/ul commercial Temperature packaging
packaging; min
200 gm/pack
Peraturan 11 Part IV
* Polygalacturonase Chilled/Room Any suitable
Tomato NA NA Perishable Raw - min 1 kg None Peraturan -Peraturan
gene Temperature packaging
Makanan 1985
Sample condition
Information
perishable
Raw - min 500
gm (edible
portion without
Animal
Porcine DNA NA NA Non- products - 2 None Peraturan -Peraturan
products
commercial
packaging; min
200 gm/pack
Raw - min 1 kg
Roundup Ready® kernels & packs of Frozen/Chilled/Room Any suitable
Soybean (RRS) soybean commercial Temperature packaging
200 gm/pack
Raw - min 1 kg
* Roundup Ready® kernels & packs of Frozen/Chilled/Room Any suitable
Soybean (RRS) soybean commercial Temperature packaging
200 gm/pack
Sample condition
Information
perishable
Raw - min 1 kg
Products - 2
Tomato and packs of Frozen/Chilled/Room Any suitable
* Zeneca® F282 gene 0.1% NA Non- None Peraturan -Peraturan
its products commercial Temperature packaging
packaging; min
200 gm/pack
4. Mycotoxin
Sample condition
MRL (Reference)
Food LOD LOQ
Unit Analyte Group Analytes /Guideline
Matrix (Unit) (Unit)
Sample Perishable / Other (Reference)
Temperature Packaging
weight Non perishable Information
Paper / Plastic 1. Food Act &
Aflatoxin (Sum bag or Regulation;
2.4 Non Room
B1,B2,G1 and Cereal 1.2 1kg container / NA Fifthteenth
ug/kg perishable temperature
G2) original Schedule (Table
packaging II)
Aflatoxin (Sum bag or Regulation;
2.4 Non Room
B1,B2,G1 and Spices 1.2 1kg container / NA Fifthteenth
ug/kg perishable temperature
G2) original Schedule (Table
packaging II)
1.5 kg
(with Paper / Plastic 1. Food Act &
Mycotoxin
Aflatoxin (Sum shell/null) bag or Regulation;

Peanut & 2.4 Non Room
Aflatoxin B1,B2,G1 and 1.2 container / NA Fifthteenth
Rice ug/kg perishable temperature
G2) 1.0kg original Schedule (Table
(without packaging II)
shell/null)
1. Food Act &
Plastic
Regulation;
Milk - 0.02 0.05 Non Room container /
Aflatoxin M1 1L NA Fifthteenth
Liquid ug/kg ug/kg perishable temperature original
Schedule (Table
packaging
II)
bag or Regulation;
Milk - 0.07 0.25 Non Room
Aflatoxin M1 1kg container / NA Fifthteenth
Powder ug/kg ug/kg perishable temperature
original Schedule (Table
packaging II)
Page 175
Sample condition
MRL (Reference)
Food LOD LOQ
Unit Analyte Group Analytes /Guideline
Matrix (Unit) (Unit)
Sample Perishable / Other (Reference)
Temperature Packaging
weight Non perishable Information
Paper / Plastic
Rice,
bag or
Maize & 30 100 Non Room No
Deoxynivalenol Deoxynivalenol 1kg container / NA
Wheat ug/kg ug/kg perishable temperature MRL/Guidelines
original
Products
packaging
bag or Regulation;
Rice & 0.2 0.5 Non Room
Ochratoxin A 1kg container / NA Fifthteenth
Wheat ug/kg ug/kg perishable temperature
packaging II)
Ochratoxin A
bag or Regulation;
0.2 0.5 Non Room
Ochratoxin A Coffee 1kg container / NA Fifthteenth
ug/kg ug/kg perishable temperature
packaging II)
Paper / Plastic
Rice,
bag or
maize & 5 18 Non Room No
Zearalenone Zearalenone 1kg container / NA
wheat ug/kg ug/kg perishable temperature MRL/Guidelines
original
product
packaging
Note:* No SAMM Accreditation
C. The Comprehensive List of Food Division Tests in NPHL (Mycotoxin) Page 176
5. Industrial Pollution and Food Environment (Pencemaran Industri dan Persekitaran Makanan, PIPM)
Sample condition
Analyte LOD LOQ MRL (Reference)

Unit Analytes Food Matrix
Group (Unit) (Unit) Type of /Guideline (Reference)
Sample Other
Samples/ Temperature Packaging
weight Information
Parameter
a) Chilled if
Pencemaran Industri dan Persekitaran Makanan (PIPM)
send within Plastic/ Any samples

24hrs Paper/ for Heavy
Perishable 1 kg or 1 L Metals
b) Frozen if Original
send after 24 Packaging analysis
Food Act & Regulation;
0.02 0.05 hrs should not
Antimony Antimony Soft Drink Fourteenth Schedule
mg/kg mg/kg have direct
(Table 1)
contact with
Plastic /
Non- 500g or Room any metals
Original
Perishable 500ml Temperature container or
Packaging
apparatus
a) Chilled if
Any samples
send within Plastic/
for Heavy
24hrs Paper/
Perishable 1 kg or 1 L Metals
analysis
send after 24 Packaging Food Act & Regulation;
0.3 0.8 should not
Arsenic (As) Total Arsenic Food hrs Fourteenth Schedule
(Table 1 & 1A)
contact with
Plastic /
Original
Packaging
apparatus
a) Chilled if Any samples 1. Food Act &
send within Plastic/ for Heavy Regulation; Fourteenth
Cadmium 0.02 0.07 24hrs Paper/ Metals Schedule (Table 1 & 1E)
Cadmium a) Fish Perishable 1 kg or 1 L
(Cd) mg/kg mg/kg b) Frozen if Original analysis 2. Commission
send after 24 Packaging should not Regulation (EC) No.
hrs have direct 1881/2006
Page 177
Sample condition

Sample Other
weight Information
Parameter
contact with
any metals
Plastic /
Non- 500g or Room container or
Original
Perishable 500ml Temperature apparatus
Packaging
Any samples
for Heavy
Metals
b) Plastic analysis
10 pieces Plastic / Food Act & Regulation;
(Food Ceramic/ Room should not
* Cadmium NA 25 mg/kg from same Original Thirteenth Schedule
Contact Plastic ware Temperature have direct
lot Packaging (Table 1)
Material) contact with
any metals
container or
apparatu
a) Chilled if
send within Plastic/
Any samples
24hrs Paper/
Perishable 1 kg or 1 L for Heavy
Metals 1. Food Act &
send after 24 Packaging
analysis Regulation; Fourteenth
hrs
0.003 0.01 should not Schedule (Table 1 & 1E)
Cadmium Fish
mg/kg mg/kg have direct 2. Commission
contact with Regulation (EC) No.
Plastic / any metals 1881/2006
Non- 500g or Room
Original container or
Perishable 500ml Temperature
Packaging apparatus
C. The Comprehensive List of Food Division Tests in NPHL (Industrial Pollution and Food Environment) Page 178
Sample condition

Sample Other
weight Information
Parameter
a) Chilled if
Perishable 1 kg or 1 L
b) Frozen if Original Metals
send after 24 Packaging analysis
Food Act & Regulation;
0.03 0.1 hrs should not
Methyl Mercury Fish Fourteenth Schedule
(Table 1D)
contact with
Plastic /
Original
Packaging
apparatus
a) Chilled if Any samples

Mercury send within Plastic/ for Heavy
(Hg) 24hrs Paper/ Metals
b) Frozen if Original analysis
Fish & fish 0.01 0.04 send after 24 Packaging should not Commission Regulation
product mg/kg mg/kg hrs have direct (EC) No. 1881/2006
contact with
Plastic /
Total Mercury Original
Packaging
apparatus
a) Chilled if Any samples
send within Plastic/ for Heavy
Vegetable & Food Act & Regulation;
0.01 0.04 24hrs Paper/ Metals
veg. Perishable 1 kg or 1 L Fourteenth Schedule
mg/kg mg/kg b) Frozen if Original analysis
product (Table 1 & 1D)
send after 24 Packaging should not
hrs have direct
Sample condition

Sample Other
weight Information
Parameter
contact with
Plastic /
Original
Packaging
apparatus
a) Chilled if
b) Frozen if Original Metals 1. Food Act &
send after 24 Packaging analysis Regulation; Fourteenth
0.01 0.03 hrs should not Schedule (Table 1 & 1B)
Plumbum a) Food
mg/kg mg/kg have direct 2. Commission
contact with Regulation (EC) No.
Plastic / any metals 1881/2006
Non- 500g or Room
Original container or
Perishable 500ml Temperature
Plumbum Packaging apparatus
(Pb)
Any samples
for Heavy
Metals
b) Plastic analysis
10 pieces Plastic / Food Act & Regulation;
(Food Ceramic/ Room should not
* Plumbum NA 25 mg/kg from same Original Thirteenth Schedule
Contact Plastic ware Temperature have direct
lot Packaging (Table 1)
Material) contact with
any metals
container or
apparatu
Sample condition

Sample Other
weight Information
Parameter
Any samples
for Heavy
a) Chilled if Metals 1. Food Act &
send within Plastic/ analysis Regulation; Fourteenth
Fish & fish 0.7 24hrs Paper/ should not Schedule (Table 1C)
Stanum 10 mg/kg Perishable 1 kg or 1 L
product mg/kg b) Frozen if Original have direct 2. Commission
send after 24 Packaging contact with Regulation (EC) No.
hrs any metals 1881/2006
container or
Stanum apparatus
(Tin) Any samples
for Heavy
a) Chilled if Metals 1. Food Act &
send within Plastic/ analysis Regulation; Fourteenth
0.2 24hrs Paper/ should not Schedule (Table 1C)
Stanum Fish 10 mg/kg Perishable 1 kg or 1 L
mg/kg b) Frozen if Original have direct 2. Commission
send after 24 Packaging contact with Regulation (EC) No.
hrs any metals 1881/2006
container or
apparatus
6. Natural Contamination (Pencemaran Semulajadi)
Sample Condition
MRL (Reference)
LOD LOQ
Unit Analyte Group Analytes Food Matrix Perishable/ /Guideline
(Unit) (Unit) Sample Other
non Temperature Packaging (Reference)
weight Information
perishable
Plastic/
Rice and rice 2.0 100 Non Room
Bromide* 1 kg commercial NA No MRL/Guidelines
products mg/kg mg/kg perishable temperature
pack
Plastic/
Rice and rice 2.0 100 Non Room
Chloride* 1 kg commercial NA No MRL/Guidelines
products mg/kg mg/kg perishable temperature
pack
Pencemaran Semulajadi
Anion Room
temperature/ Commission
Vegetable & 20 50 Plastic bag/
Nitrate* 1 kg Perishable chilled (if NA Regulation (EC)
veg. product mg/kg mg/kg brown paper
transport more 1881/2006.
than 24 hours)
Room
temperature/ Commission
Vegetable & 20 50 Plastic bag/
Nitrite* 1 kg Perishable chilled (if NA Regulation (EC)
veg. product mg/kg mg/kg brown paper
transport more 1881/2006.
than 24 hours)
Original Peraturan 11 (5),

All foods Perishable :
2.5 Frozen or container/ Part IV, Peraturan -
Alergen Egg except food NA 500 g 500 g @ NA
ppm chilled suitable Peraturan
contain egg 500 ml
packages Makanan 1985
Page 182
Sample Condition
MRL (Reference)
LOD LOQ
weight Information
perishable
Non- Original
Perishable : Room container/
NA
500g @ temperature suitable
500ml packages
Original
Perishable :
Frozen or container/
500 g @ NA
chilled suitable
All foods 500 ml Peraturan 11 (5),
packages
except food Part IV, Peraturan -
Gliadin NA 5 ppm 500 g
contain Peraturan
gliadin/gluten Non- Original Makanan 1985
NA
500ml packages
Original
Perishable :
500 g @ NA
chilled suitable
500 ml Peraturan 11 (5),
All foods packages
2.5 Part IV, Peraturan -
Milk except food NA 500 g
ppm Non- Original Peraturan
contain milk
Perishable : Room container/ Makanan 1985
NA
500ml packages
C. The Comprehensive List of Food Division Tests in NPHL (Natural Contamination) Page 183
Sample Condition
MRL (Reference)
LOD LOQ
weight Information
perishable
Original
Perishable :
500 g @ NA
chilled suitable
All foods packages
Peanut except food NA 500 g
ppm Peraturan
contain peanut Non- Original
Makanan 1985
NA
500ml packages
Original
Perishable :
500 g @ NA
chilled suitable
All foods packages
Soy* except food NA 500 g
ppm Peraturan
contain soy Non- Original Makanan 1985
NA
500ml packages
6.0 50
Cadaverine
mg/kg mg/kg
6.0 50
Histamine Frozen or Plastic/ Commission
mg/kg mg/kg
Biogenic Amines Fish 500 g Perishable chilled where commercial NA Regulation (EC) No.
6.0 50
2-phenylethylamine applicable pack 2073/2005
mg/kg mg/kg
6.0 50
Putrescine
mg/kg mg/kg
Sample Condition
MRL (Reference)
LOD LOQ
weight Information
perishable
6.0 50
Spermidine
mg/kg mg/kg
6.0 50
Spermine
mg/kg mg/kg
6.0 50
Tryptamine
mg/kg mg/kg
Original
0.02 0.06 Non- Room container/
4-methylimidazole 4-methylimidazole Drinks 500 mL NA EU = 250 mg/kg
mg/kg mg/kg Perishable temperature suitable
packages
2.0
Bromide 5 mg/L
mg/L
1. 25th Schedule,
4.0 12.5 Original 25th A Schedule &
Cloride
Mineral Water/ mg/L mg/L Non- Room container/ 26th Schedule
Anion 500 mL NA
Drinking water Perishable temperature suitable Food Regulation
0.2 0.5 packages 1985.
Fluoride
mg/L mg/L 2. EU Regulation
2.0
Nitrate 5 mg/L
mg/L
Sample Condition
MRL (Reference)
LOD LOQ
weight Information
perishable
0.2 0.5
Nitrite*
mg/L mg/L
2.0
Phosphate 5 mg/L
mg/L
4.0 12.5
Sulphate
mg/L mg/L
7. Drug Residue
Sample condition MRL

Food (Reference)
Unit Analyte Group Analytes LOD (Unit) LOQ (Unit)
Matrix Perishable /Guideline
Other
/ non Sample weight Temperature Packaging (Reference)
Information
perishable
1. Fifteenth
SDZ: 30 SDZ: 40 A Schedule
µg/kg µg/kg (Regulation
STZ: STZ: 40) Food Act
25µg/kg 40µg/kg 1983 (Act
Sulfadiazine (SDZ), Sample
SMR: 17 SMR: 281) &
Sulfathiazole(STZ), should be
µg/kg 20µg/kg Clean, Regulations,
Sulfamerazine (SMR), sent to the
Meat & SDD: 25 SDD: 40 chemically 2. COUNCIL
Sulfadimidine (SDD), Chilled / laboratory
Meat µg/kg µg/kg inert and REGULATION
Sulfamonomethoxine Frozen / as soon as
Products, SMM: 20 SMM: Meat: 500g light-proof (EEC) No
Antibacterial (SMM), Perishable Room possible,
Fish & µg/kg 30µg/kg Fish: 1000g container / 2377/90
Oxolinic Acid (OXA) Temperature after taking
Fish OXA: 25 OXA: 40 Commercial of 26 June
Sulfadimethoxine (products) precautions
Products µg/kg µg/kg packing 1990
(SDM), against
SDM: 30 SDM: 40 (products) 3. Table #A-
Drug Residue
Sulfaquinoxaline (SQX), leakage and

µg/kg µg/kg 5. FDA & EPA
Nicarbazine (NCZ), spoilage
SQX: 30 SQX: 40 safety levels
µg/kg µg/kg in
NCZ: 10 NCZ: 20 regulations
µg/kg µg/kg and
guidance.
Sample
should be
sent to the
COUNCIL
Clean, laboratory
REGULATION
chemically as soon as
EMA: 0.3 EMA: 1 Chilled / (EEC) No
*Avermectin Emamectin (EMA) Fish Perishable 1000 g inert and possible,
µg/kg µg/kg Frozen 2377/90
light-proof after taking
of 26 June
container precautions
1990
against
leakage and
spoilage
Page 187
Food (Reference)
Other
Information
perishable
2AA: 0.29
µg/kg
ALB: 0.22
µg/kg
1. Fifteenth
ASF: 0.32
2-amino-albendazole Sample A Schedule
µg/kg
sulfone, should be (Regulation
ASOX: 0.38 Clean,
Albendazole sulfone, sent to the 40) Food Act
µg/kg chemically
Fenbendazole, Chilled / laboratory 1983 (Act
FEBAN: inert and
Oxfendazole, Fish & Frozen / as soon as 281) &
0.83 µg/kg Meat: 500g light-proof
Benzimidazole Thiabendazole, Fish 1 µg/kg Perishable Room possible, Regulations,
FEN: 0.76 Fish: 1000g container /
Albendazole, Products Temperature after taking 2. COUNCIL
µg/kg Commercial
Flubendazole , (products) precautions REGULATION
FLUB: 0.47 packing
Mebendazole, against (EEC) No
µg/kg (products)
Albendazole Sulfoxide, leakage and 2377/90
MEB: 0.21
Febantel spoilage of 26 June
µg/kg
1990
OXFEN:
0.70 µg/kg
TBZ: 0.48
µg/kg
CIMA: 0.1 CIMA: 0.5
Sample
µg/kg µg/kg
should be
CLEN: 0.1 CLEN: 0.5 Clean,
sent to the Fifteenth A
Cimaterol (CIMA), µg/kg µg/kg chemically
Chilled / laboratory Schedule
Clenbuterol (CLEN), MABU: 0.2 MABU: 0.5 inert and
Meat and Frozen / as soon as (Regulation
Mabuterol (MABU), µg/kg µg/kg light-proof
Beta-agonist Meat Perishable 500 g Room possible, 40) Food Act
Terbutaline (TERB), TERB: 0.4 TERB: 1 container /
Products Temperature after taking 1983 (Act
Ractopamine (RACTO), µg/kg µg/kg Commercial
(products) precautions 281) &
Salbutamol (SAL) RACTO: 0.1 RACTO: 0.5 packing
against Regulations
µg/kg µg/kg (products)
leakage and
SAL: 0.4 SAL: 1
spoilage
µg/kg µg/kg
C. The Comprehensive List of Food Division Tests in NPHL (Drug Residue) Page 188
Food (Reference)
Other
Information
perishable
Quantity 1. Fifteenth
needed do A Schedule
Amber
not include (Regulation
CAP: 0.05 CAP: 0.2 Non 250 mL / 250 Room bottle /
Chloramphenicol Chloramphenicol Honey the 40) Food Act
µg/kg µg/kg perishable g Temperature Commercial
packaging 1983 (Act
packing
or inedible 281) &
portion Regulations
Food (Reference)
Other
Information
perishable
DANO: 10
µg/kg
DANO:
TILMI: 10
0.23 µg/kg
µg/kg
TILMI: 3.4
DIFLO: 5 1. Fifteenth
µg/kg
µg/kg A Schedule
DIFLO: 0.9
Danofloxacin (DANO), TMP: 5 (Regulation
µg/kg
Tilmicosin (TIL), µg/kg 40) Food Act
TMP: 1.0
Difloxacin (DIFLO), SDZ: 2 1983 (Act
µg/kg Sample
Trimetoprim (TMP), µg/kg 281) &
SDZ: 0.7 should be
Sulfadiazine (SDZ), SMM: 2 Clean, Regulations,
µg/kg sent to the
Sulfamonomethoxine µg/kg chemically 2. COUNCIL
SMM: 0.7 Chilled / laboratory
(SMM), SDD: 2 inert and REGULATION
Fish and µg/kg Frozen / as soon as
*Multi residue Drug Sulfadimidine (SDD), µg/kg Meat: 500g light-proof (EEC) No
Fish SDD: 0.7 Perishable Room possible,
(ATB2) Sulfathiazole(STZ), STZ: 1 Fish: 1000g container / 2377/90
Product µg/kg Temperature after taking
Sulfadimethoxine µg/kg Commercial of 26 June
STZ: 0.3 (products) precautions
(SDM), SDM: 1 packing 1990
µg/kg against
Sulfaquinoxaline (SQX), µg/kg (products) 3. Table #A-
SDM: 0.2 leakage and
Sulfapyridine (SPD), SQX: 1 5. FDA & EPA
µg/kg spoilage
Sulfamerazine (SMR), µg/kg safety levels
SQX: 0.3
Oxolinic acid (OXA), SPD: 1 in
µg/kg
Ormetoprim (OMP) µg/kg regulations
SPD: 0.2
SMR: 1 and
µg/kg
µg/kg guidance.
SMR: 0.3
OXA: 10
µg/kg
µg/kg
OMP: 1
µg/kg
Food (Reference)
Other
Information
perishable
ALB: 50 ALB: 50
µg/kg µg/kg
1. Fifteenth
OXFEN: 50 OXFEN: 50
Sample A Schedule
µg/kg µg/kg
Albendazole (ALB), should be (Regulation
TBZ: 50 TBZ: 50
Oxfendazole (OXFEN), Clean, sent to the 40) Food Act
µg/kg µg/kg
Thiabendazole (TBZ), chemically laboratory 1983 (Act
CAP: 0.2 CAP: 0.2
Chloramphenicol (CAP), Room inert and as soon as 281) &
Multi residue of µg/kg µg/kg 500 mL / 500
Thiamphenicol (TAP), Milk Perishable Temperature light-proof possible, Regulations,
Veterinary Drug in milk TAP: 0.5 TAP: 0.5 g
Florfenicol (FF), / Chilled container / after taking 2. COUNCIL
µg/kg µg/kg
Sulphadimidine (SDD), Commercial precautions REGULATION
FF: 0.5 FF: 0.5
Sulphadimethoxine packing against (EEC) No
µg/kg µg/kg
(SDM) leakage and 2377/90
SDD: 10 SDD: 10
spoilage of 26 June
µg/kg µg/kg
1990
SDM: 5 SDM: 5
µg/kg µg/kg
1. Fifteenth
Sample A Schedule
should be (Regulation
3-amino-2- Clean,
AOZ: 0.3 AOZ: 0.5 sent to the 40) Food Act
oxazolidinone (AOZ), 3- Meat & chemically
µg/kg µg/kg Chilled / laboratory 1983 (Act
amino-5- Meat inert and
Frozen / as soon as 281) &
morpholinomethyl-2- Products, Meat: 500g light-proof
Nitrofuran AMOZ: 0.2 AMOZ: 0.5 Perishable Room possible, Regulations,
oxazolidinone (AMOZ), Fish & Fish: 1000g container /
µg/kg µg/kg Temperature after taking 2. COUNCIL
Semicarbazide (SEM), Fish Commercial
SEM: 0.5 SEM: 1 (products) precautions REGULATION
*1-aminohydantoin Products packing
µg/kg µg/kg against (EEC) No
(AHD) (products)
leakage and 2377/90
spoilage of 26 June
1990
Food (Reference)
Other
Information
perishable
1. Fifteenth
Sample A Schedule
Clean,
sent to the 40) Food Act
Dimetridazole HMMNI: HMMNI: 1 chemically
Chilled / laboratory 1983 (Act
metabolites (HMMNI), 0.2 µg/kg µg/kg inert and
Fish & Frozen / as soon as 281) &
Metronidazole (MNZ), MNZ: 0.2 MNZ: 0.5 Meat: 500g light-proof
Nitroimidazole Fish Perishable Room possible, Regulations,
Ronidazole (RNZ) µg/kg µg/kg Fish: 1000g container /
Products Temperature after taking 2. COUNCIL
*Hydroxymetronidazole RNZ: 0.05 RNZ: 0.2 Commercial
(products) precautions REGULATION
(MNZ-OH) µg/kg µg/kg packing
against (EEC) No
(products)
leakage and 2377/90
spoilage of 26 June
1990
SDZ: 40 SDZ: 40
µg/kg µg/kg
1. Fifteenth
STZ: 40 STZ: 40
A Schedule
Sulfadiazine (SDZ), µg/kg µg/kg
(Regulation
Sulfathiazole(STZ), SPD: 40 SPD: 40
Clean, Quantity 40) Food Act
Sulfapyridine (SPD), µg/kg µg/kg
Room chemically needed do 1983 (Act
Sulfamerazine (SMR), SMR: SMR:
Temperature inert and not include 281) &
Sulfadimidine (SDD), 40µg/kg 40µg/kg Non
*Sulfonamides Egg 500g / 500 mL / Chilled / light-proof the Regulations,
Sulfamonomethoxine SDD: 20 SDD: 20 Perishable
Frozen container / packaging 2. COUNCIL
(SMM), µg/kg µg/kg
(products) commercial or inedible REGULATION
Sulfadimethoxine SMM: SMM:
packing portion (EEC) No
(SDM), Sulfaquinoxaline 40µg/kg 40µg/kg
2377/90
(SQX) SDM: 20 SDM: 20
of 26 June
µg/kg µg/kg
1991
SQX: 40 SQX: 40
µg/kg µg/kg
Food (Reference)
Other
Information
perishable
1. Fifteenth
Sample A Schedule
Clean,
Meat & CAP: 0.1 CAP: 0.2 chemically
Meat µg/kg µg/kg inert and
Chloramphenicol (CAP), Frozen / as soon as 281) &
Products, TAP: 0.2 TAP: 0.5 Meat: 500g light-proof
Phenicol Thiamphenicol (TAP), Perishable Room possible, Regulations,
Fish & µg/kg µg/kg Fish: 1000g container /
Florfenicol (FF) Temperature after taking 2. COUNCIL
Fish FF: 0.2 FF: 0.5 Commercial
Products µg/kg µg/kg packing
against (EEC) No
(products)
leakage and 2377/90
spoilage of 26 June
1990
1. Fifteenth
CIPRO: 2.6 CIPRO: 5
Sample A Schedule
µg/kg µg/kg
Ciprofloxacin (CIPRO), should be (Regulation
ENRO: 2.6 ENRO: 5 Clean,
Enrofloxacin (ENRO), sent to the 40) Food Act
Meat & µg/kg µg/kg chemically
Danofloxacin (DANO), Chilled / laboratory 1983 (Act
Meat DANO: 2.3 DANO: 5 inert and
Sarafloxacin (SARA), Frozen / as soon as 281) &
Products, µg/kg µg/kg Meat: 500g light-proof
Quinolone Marbofloxacin Perishable Room possible, Regulations
Fish & SARA: 2.0 SARA: 5 Fish: 1000g container /
(MARBO), Temperature after taking 2. COUNCIL
Fish µg/kg µg/kg Commercial
Norfloxacin (NORFLO), (products) precautions REGULATION
Products MARBO: MARBO: 5 packing
*Difloxacin (DIFLO), against (EEC) No
2.7 µg/kg µg/kg (products)
*Oxolinic acid (OXA) leakage and 2377/90
NORFLO: NORFLO: 5
spoilage of 26 June
1.7 µg/kg µg/kg
1990
Food (Reference)
Other
Information
perishable
1. Fifteenth
Sample A Schedule
Clean,
Meat & SMR : 0.33 SMR : 0.5 chemically
Sulfamerazine (SMR), Meat ug/kg ug/kg inert and
Sulfamerazine, Frozen / as soon as 281) &
Sulfadimethoxine Products, SDM : 0.53 SDM : 1.0 Meat: 500g light-proof
Sulfadimethoxine and Perishable Room possible, Regulations,
(SDM), Fish & ug/kg ug/kg Fish: 1000g container /
Ormetoprim Temperature after taking 2. COUNCIL
Ormetoprim (OMP) Fish OMP : 0.12 OMP : 0.5 Commercial
Products ug/kg ug/kg packing
against (EEC) No
(products)
leakage and 2377/90
spoilage of 26 June
1990
SDZ: 1.5 SDZ : 5
µg/kg µg/kg
SMM: 2.4 SMM: 10
Sulfadiazine (SDZ), µg/kg µg/kg
Sulfamonomethoxine SDD: 2.4 SDD: 10
Quantity
(SMM), µg/kg µg/kg
needed do
Sulfadimidine (SDD), STZ: 2.6 STZ: 10 Amber
not include
Sulfathiazole(STZ), µg/kg µg/kg Non 250 mL / 250 Room bottle /
Sulfonamides Honey the nil
Sulfadimethoxine SDM: 0.2 SDM: 1 Perishable g Temperature Commercial
packaging
(SDM), µg/kg µg/kg packing
or inedible
Sulfaquinoxaline (SQX), SQX: 0.7 SQX: 5
portion
Sulfapyridine (SPD), µg/kg µg/kg
Sulfamerazine (SMR) SPD: 2.8 SPD: 10
µg/kg µg/kg
SMR: 3.4 SMR: 10
µg/kg µg/kg
Food (Reference)
Other
Information
perishable
1. Fifteenth
A Schedule
(Regulation
40) Food Act
1983 (Act
Sample
281) &
should be
TC: 30 Clean, Regulations,
sent to the
Meat & TC: 9 µg/kg µg/kg chemically 2. COUNCIL
Chilled / laboratory
Tetracycline(TC), Meat OTC: 8 OTC: 30 inert and REGULATION
Frozen / as soon as
Oxytetracycline (OTC), Products, µg/kg µg/kg Meat: 500g light-proof (EEC) No
Tetracyclines Perishable Room possible,
Doxycyline (DC), Fish & DC: 8 µg/kg DC: 30 Fish: 1000g container / 2377/90
Temperature after taking
Chlortetracycline (CTC) Fish CTC: 6 µg/kg Commercial of 26 June
(products) precautions
Products µg/kg CTC: 30 packing 1991
against
µg/kg (products) 3. Table #A-
leakage and
5. FDA & EPA
spoilage
safety levels
in
regulations
and
guidance.
Sample
should be
CV: 0.17 CV: 0.5 Clean,
sent to the
µg/kg µg/kg chemically COUNCIL
Crystal Violet (CV), Chilled / laboratory
LCV: 0.19 LCV: 0.5 inert and REGULATION
Leucrystal Violet (LCV), Fish & Frozen / as soon as
Triphenylmethane µg/kg µg/kg light-proof (EEC) No
Malachite Green (MG), Fish Perishable 1000 g Room possible,
Dyes MG: 0.15 MG: 0.5 container / 2377/90
Leucomalachite Green Products Temperature after taking
µg/kg µg/kg Commercial of 26 June
(LMG), (products) precautions
LMG: 0.23 LMG: 0.5 packing 1991
against
µg/kg µg/kg (products)
leakage and
spoilage
8. Pesticide Residue
Sample condition
MRL
LOD LOQ (Reference)
Unit Analyte Group Analytes Food Matrix Perishable/
(Unit) (Unit) Sample Other /Guideline
Non Temperature Packaging (Reference)
weight Information
Perishable
Azinphos ethyl * 0.0075 0.025
Carbofenothion 0.0033 0.010
Chlorpyrifos 0.0083 0.025
Chlorpyrifos methyl 0.0083 0.025
Cyanofenphos 0.0083 0.025
Diazinon 0.0033 0.010
Dichlorvos (DDVP) 0.0075 0.025
Dimethoate * 0.0075 0.025
Jadual
Ethion 0.0083 0.025 Keenam Belas
Fenitrothion 0.0083 0.025 dan Peraturan
Residu Racun Perosak
Brown
Fensulfothion * 0.0075 0.025 paper/ 41,
Fenthion * Fruit and 0.0075 0.025 Room plastic bags Peraturan-
1kg Perishable None peraturan
Organophosphorus Formothion * Vegetables 0.0075 0.025 Temperature with
ventilation Makanan
Malathion 0.0067 0.020 1985, Akta
hole
Methamidophos * 0.0075 0.025 Makanan
Methidathion * 0.003 0.010 1983 serta
Parathion ethyl * CODEX
0.0075 0.025
Alimentarius
Parathion methyl 0.0167 0.050
Phosalone * 0.015 0.050
Pirimiphos methyl 0.0033 0.010
Profenophos 0.0083 0.025
Prothiophos 0.0083 0.025
Quinalphos 0.0067 0.020
Tolclophos methyl 0.0083 0.025
Chlorpyrifos 0.001 0.010 1 kg None
Page 196
Sample condition
MRL
LOD LOQ (Reference)
weight Information
Perishable
Chlorpyrifos-methyl* 0.0075 0.025

Diazinon 0.001 0.010
Dichlorvos (DDVP) 0.001 0.010
Dimethoate* 0.0075 0.025
Ethion 0.001 0.010
Fenitrothion 0.001 0.010
Fenthion* 0.0075 0.025
Formothion 0.002 0.025
Brown
Malathion Cereal, 0.001 0.010 paper/
Non Room
Methamidophos* Bean and 0.0075 0.025 Plastic /
Perishable Temperature
Methidathion Seed 0.002 0.025 Original
Parathion-ethyl* packaging
0.003 0.010
Parathion-methyl 0.001 0.010
Phenthoate* 0.0075 0.025
Phosalone 0.002 0.025
Pirimiphos-methyl 0.019 0.010
Prothiophos 0.001 0.010
Tolclofos-methyl 0.002 0.025
Clean and
Diazinon 0.018 0.06 chemically
Room
Dichlorvos 0.018 0.06 inert
Milk 500g Perishable Temperature / None
Dimethoate 0.018 0.06 container/
Chilled
Commercial
Ethion 0.018 0.06
packaging
Fenthion 0.018 0.06
C. The Comprehensive List of Food Division Tests in NPHL (Pesticide Residue) Page 197
Sample condition
MRL
LOD LOQ (Reference)
weight Information
Perishable
Malathion 0.018 0.06
Parathion Ethyl 0.018 0.06
Acephate 0.041 0.10
Azinphos-methyl 0.078 0.30
Bromophos-methyl 0.022 0.07
Chlorfenvinphos 0.013 0.04
Coumaphos 0.023 0.08
Demeton-s-methyl 0.03 0.10
Dichlofenthion 0.01 0.04
Dimethoate 0.007 0.02 Brown
Ethoprophos 0.008 0.03 paper/
Room plastic bags
Fenamiphos Pome Fruit 0.014 0.05 1kg Perishable None
Temperature with
Fenchlorphos 0.005 0.02 ventilation
Methamidophos 0.027 0.09 hole
Monocrotophos 0.039 0.10
Omethoate 0.095 0.30
Phorate 0.012 0.04
Phosmet 0.035 0.10
Pirimiphos-ethyl 0.02 0.07
Triazophos 0.007 0.03
Trichlorfon 0.02 0.07
Acephate 0.12 0.40
Brown
Azinphos-methyl 0.17 0.60 paper/
Brassica
Bromophos-methyl 0.01 0.03 Room plastic bags
(cole) 1kg Perishable None
Chlorfenvinphos 0.05 0.20 Temperature with
vegetables
Coumaphos 0.12 0.40 ventilation
hole
Demeton-s-methyl 0.3 1.00
Sample condition
MRL
LOD LOQ (Reference)
weight Information
Perishable
Dichlofenthion 0.005 0.02
Dimethoate 0.01 0.03
Ethoprophos 0.01 0.02
Fenamiphos 0.01 0.05
Fenchlorphos 0.003 0.02
Methamidophos 0.02 0.06
Monocrotophos 0.21 1.00
Omethoate 0.12 0.40
Phorate 0.004 0.02
Phosmet 0.08 0.30
Pirimiphos-ethyl 0.01 0.04
Trichlorfon 0.01 0.04
Azinphos-ethyl 0.01 0.01
Carbofenothion 0.01 0.01
Chlorpyrifos-methyl 0.01 0.01
Diazinon 0.01 0.01
Dichlorvos Leafy 0.01 0.02
1kg
Ethion Vegetables 0.01 0.01
Fenitrothion 0.01 0.01
Fensulfothion 0.01 0.01
Fenthion 0.01 0.02
Formothion 0.01 0.01
Malathion 0.01 0.01
Methidathion 0.01 0.01
Sample condition
MRL
LOD LOQ (Reference)
weight Information
Perishable
Parathion-ethyl 0.01 0.03
Parathion-methyl 0.01 0.04
Phenthoate 0.01 0.02
Phosalone 0.01 0.03
Protiophos 0.01 0.01
Tolclophos-methyl 0.01 0.02
Azinphos-ethyl 0.018 0.06
Chlorfenvinphos 0.018 0.06
Diazinon 0.018 0.06
Methidathion 0.018 0.06 Commercial
Non Room
Parathion-ethyl Cooking Oil 0.018 0.06 1kg/ 1L packaging/ None
Parathion-methyl 0.018 0.06 Plastic
Profenofos 0.018 0.06
2,4-DDT 0.0083 0.025
4,4-DDD 0.0083 0.025
4,4-DDE 0.0083 0.025 Brown
4,4-DDT 0.0083 0.025 paper/
Fruit and Room plastic bags
Organochlorine Aldrin * 0.0075 0.025 1kg Perishable None
Vegetables Temperature with
Captafol * 0.075 0.250 ventilation
Captan * 0.075 0.250 hole
Dichloran 0.0033 0.010
Dieldrin 0.0033 0.010
Sample condition
MRL
LOD LOQ (Reference)
weight Information
Perishable
Endrin 0.0033 0.010
Gamma-HCH 0.0017 0.005
Heptachlor 0.0017 0.005
Quintozene 0.0017 0.005
Triademefon * 0.015 0.050
Triflumuron 0.0083 0.025
2,4-DDT* 0.003 0.010
4,4-DDD* 0.003 0.010
4,4-DDE* 0.003 0.010
4,4-DDT* 0.003 0.010
Aldrin* 0.003 0.010
Dichloran* Cereal, Brown
0.003 0.010 Non Room
Bean and 1kg paper/ None
Dieldrin* 0.003 0.010 Perishable Temperature
Seed plastic
Endrin* 0.003 0.010
Gamma-HCH* 0.0075 0.025
Heptachlor* 0.003 0.010
Quintozene* 0.003 0.010
Triademefon* 0.0075 0.025
Aldrin 0.0009 0.003
Chlordane 0.0009 0.003
DDT 0.0009 0.003
Glass Bottle /
Dieldrin 0.0009 0.003 Non
Water 5L 4-10⁰C Commercial None
Gamma HCH 0.0009 0.003 Perishable
packaging
Hexachlorobenzene 0.0009 0.003
Metoxychlor 0.0009 0.003
2,4'-DDT Tea 0.01 0.05 500g None
Sample condition
MRL
LOD LOQ (Reference)
weight Information
Perishable
4,4'-DDD 0.01 0.05
4,4'-DDE 0.01 0.05
Alpha-endosulfan 0.01 1
Beta-endosulfan 0.01 1
Cypermethrin 0.2 1 Brown
Deltamethrin 0.2 1 paper/
Non Room
Plastic/
Dichloran 0.01 0.05 Perishable Temperature
Commercial
Dieldrin 0.01 0.05 packaging
Endosulfan sulfate 0.01 1
Fenvalerate 0.2 0.5
Gamma-HCH 0.01 0.05
4,4-DDD 0.004 0.010
4,4-DDE 0.003 0.010
4,4-DDT 0.004 0.010
Aldrin 0.003 0.010
Alpha -BHC 0.003 0.010
0.003 0.010 1kg
Alpha-chlordane
(except Aluminium
Beta-BHC Fish, Meat 0.004 0.010
fish or foil and
Delta-BHC and Its 0.003 0.010 Perishable Frozen/Chilled None
meat plastic/
Products
Dieldrin 0.003 0.010 products plastic
Endrin 0.004 0.010 500g)
Gamma-BHC 0.003 0.010
Gamma-chlordane 0.003 0.010
Heptachlor-exo-
0.003 0.010
epoxide
Sample condition
MRL
LOD LOQ (Reference)
weight Information
Perishable
2,4'-DDT 0.02 0.06
4,4'-DDE 0.02 0.07
4,4'-DDT 0.02 0.05
Aldrin 0.01 0.03 Brown
Dicloran 0.04 0.10 paper/
Room plastic bags
Dieldrin Pome Fruit 0.02 0.06 1kg Perishable None
Temperature with
Endrin 0.02 0.07 ventilation
Gamma-HCH 0.01 0.03 hole
2,4'-DDT 0.02 0.06
4,4'-DDE 0.02 0.07
4,4'-DDT 0.02 0.05
Aldrin 0.01 0.03 Brown
paper/
Dicloran 0.04 0.10
Dieldrin 0.02 0.06 1kg Perishable None
Endrin 0.02 0.07 ventilation
Gamma-HCH 0.01 0.03 hole
2,4'-DDT 0.003 0.010 Clean and
Room
4,4'-DDD 0.003 0.010 chemically
Milk 500g Perishable Temperature / None
4,4'-DDE 0.003 0.010 inert
Chilled
4,4'-DDT 0.003 0.010 container/
Sample condition
MRL
LOD LOQ (Reference)
weight Information
Perishable
Aldrin 0.003 0.010 Commercial
Alpha-HCH 0.003 0.010 packaging
Beta-HCH 0.003 0.010
Dieldrin 0.003 0.010
Endrin 0.003 0.010
Gamma-HCH 0.003 0.010
4,4'-DDE 0.018 0.06
Aldrin 0.018 0.06

Alpha-endosulfan 0.018 0.06
Beta-endosulfan 0.018 0.06 Commercial
Non Room
Dieldrin Cooking Oil 0.018 0.06 1kg/ 1L packaging/ None
Endosulfan sulfate 0.018 0.06 Plastic
Endrin 0.018 0.06
Gamma-HCH 0.018 0.06
Cyfluthrin
0.017 0.05
Brown
Cypermethrin paper/
0.017 0.05
1kg Perishable None
Synthetic Pyrethroid Fenvalerate Vegetables Temperature with
0.017 0.05 ventilation
Permethrin hole
0.017 0.05
Cyfluthrin* 0.0075 0.025 1kg None
Sample condition
MRL
LOD LOQ (Reference)
weight Information
Perishable
Cypermethrin* 0.015 0.050
Cereal, 0.015 0.050 Brown
Deltamethrin* Non Room
Bean and paper/
Fenvalerate* 0.015 0.050 Perishable Temperature
Seed Plastic
Permethrin* 0.0075 0.025
Cypermethrin 0.3 1 Brown
paper/
Deltamethrin 0.2 1 Non Room
Tea 500g Plastic/ None
Fenvalerate 0.2 0.5 Commercial
packaging
Cyfluthrin 0.02 0.08 Brown
paper/
Cypermethrin 0.03 0.09
Room plastic bags
Fenvalerate Pome Fruit 0.06 0.2 1kg Perishable None
Temperature with
ventilation
Permethrin 0.04 0.14
hole
Cyfluthrin 0.02 0.08 Brown
paper/
Cypermethrin 0.03 0.09
Fenvalerate 0.06 0.2 1kg Perishable None
ventilation
Permethrin 0.06 0.2
hole
Brown
paper/
Dithiocarbamates, Fruit and Room plastic bags
Dithiocarbamates 0.02 0.05 1kg Perishable None
express as CS2 Vegetables Temperature with
ventilation
hole
Bendiocarb 0.001 0.002 Brown
Fruit and Room
Carbamates Carbaryl 0.001 0.002 1kg Perishable paper/ None
Vegetables Temperature
Carbendazim 0.001 0.002 plastic bags
Sample condition
MRL
LOD LOQ (Reference)
weight Information
Perishable
Fenobucarb 0.003 0.01 with
Pirimicarb 0.001 0.002 ventilation
hole
Propamocarb 0.003 0.01
9. SPP
Sample condition
MRL (Reference)
Food
Unit Analyte Group Analytes LOD (Unit) LOQ (Unit) Perishable /Guideline
Matrix Sample Other
/ Non Temperature Packaging (Reference)
weight Information
Perishable
Original
Alcoholic Non Room Container /
Alcohol Methanol 0.2mg/L 0.6mg/L 250ml None Banned (no MRL)
beverage Perishable Temperature Commercial
Packaging
Food Act 1983 &

Food Regulations
1988 (Regulation
Original
266 (2) (b) (ii),
Coffee Non Room Container /
Caffeine 0.02% 0.02% 250g None ground coffee
products Perishable Temperature Commercial
shall contain not
Packaging
less than 0.9 per
SPP Caffeine cent of anhydrous
caffeine)
Original Food Act 1983 &

Non Room Container / Food Regulations
Caffeine Beverages 2mg/L 2mg/L 250ml None
Perishable Temperature Commercial 1988 (Regulation
Packaging 354: ≤200mg/L)
Original
Non Room Container / Commission
Melamine Melamine Biscuit 0.013mgkg 0.01mgkg 250g None
Perishable Temperature Commercial adopted
Packaging
Page 207
Sample condition
MRL (Reference)
Food
Matrix Sample Other
weight Information
Perishable
Original
Milk Non Room Container /
None
powder Perishable Temperature Commercial
Packaging
Frozen or
Plastic /
Chilled
Perishable Original
Where
Packaging Food Act 1983 &
All types of Not Not 250g or Applicable
Moisture None Food Regulations
food applicable applicable 250ml
1985
Plastic /
Non Room
Original
Packaging
Frozen or
Plastic /
Proximate Analysis Chilled
Perishable Original
Where
Packaging
Applicable Food Act 1983 &
All types of Not Not 250g or
Ash None Food Regulations
food applicable applicable 250ml
1986
Plastic /
Non Room
Original
Packaging
Frozen or
Plastic / Food Act 1983 &
All types of Not Not 250g or Chilled
Protein Perishable Original None Food Regulations
food applicable applicable 250ml Where
Packaging 1987
Applicable
C. The Comprehensive List of Food Division Tests in NPHL (SPP) Page 208
Sample condition
MRL (Reference)
Food
Matrix Sample Other
weight Information
Perishable
Plastic /
Non Room
Original
Packaging
a) Oatmeal
(Regulation 62 (2)
= ≥5%), b)
Cereal & Plastic / Other cereal &
Not Not Non Room
*Fat cereal 250g Original None cereal products
applicable applicable Perishable Temperature
products Packaging (Regulation 18B
states about
nutritional
labelling )
Frozen or Malaysia Food

Plastic / Regulations, 1985
Chilled
Perishable Original and Food Act
Where
Packaging 1983;
Applicable
a) Regulation 82,
84, 89, 90, 91,
91A, 91B, 94, 95,
Milk &
Not Not 250g or 96, 97, 100, 102,
Fat milk None
applicable applicable 250ml 103, 106, 107,
products Plastic /
Non Room 108, 111, 116,
Original 134B, 134C, 137,
Packaging 281 and 282.
b) Regulation 18B
states about
nutritional
labeling.
Sample condition
MRL (Reference)
Food
Matrix Sample Other
weight Information
Perishable
Malaysia Food Act
1983 and Food
Regulations, 1985;
Plastic / • Regulation 255=
Not Not Non Room
Fat Dry food 250g Original None ≥50%
Packaging • Regulation 18B
states about
nutritional
labelling
Malaysia Food Act

1983 and Food
Regulations, 1985;
Plastic / a) Regulation 185=
Not Not 250g or Non Room
Fat Wet food Original None ≥80%)
applicable applicable 250ml Perishable Temperature
Packaging b) Regulation 18B
states about
nutritional
labelling
Frozen or Malaysia Food

Plastic /
Chilled Regulations, 1985
Perishable Original
Where and Food Act
250g Packaging
Applicable 1983; a)
Meat & (edible
Not Not Regulation 141,
Fat meat portion None
applicable applicable 144 and 147
products without
Plastic / b) Regulation 18B
bones) Non Room
Original states about
Perishable Temperature nutritional
Packaging
labelling
Sample condition
MRL (Reference)
Food
Matrix Sample Other
weight Information
Perishable
Frozen or Malaysia Food Act

Plastic /
Chilled 1983 and Food
All type of Perishable Original
Where Regulations 1985;
*Carbohydrate and food (Milk Not Not 250g or Packaging
Applicable None Regulation 18B
Energy and Milk applicable applicable 250ml
Plastic / states about
Products) Non Room
Original nutritional
Perishable Temperature labelling
Packaging
Malaysia Food Act

1983 and Food
Plastic / Regulations 1985;
Not Not Non Room
*Total Dietary Fiber Dry food 250g Original None Regulation 18B
Packaging states about
nutritional
labelling
Frozen or Original
Chilled Container /
Perishable None
Where Commercial
Applicable Packaging Food Act 1983 &
Food Regulations
Salted 250g or
Sodium chloride Sodium chloride 1.0% 1.0% 1985 (Regulation
food 250ml
162, 163,164,166:
Original ≥15%)
Non Room Container /
None
Perishable Temperature Commercial
Packaging
10. Additive
Sample condition
LOD LOQ
Unit
Analyte Group Analytes Food Matrix Perishable/ MRL (Reference) /Guideline (Reference)
Non Temperature Packaging Information
weight
Perishable
Frozen/
3 10 Chilled 1) AM 1983, PPM 1985, Tenth Sch,
BHA Perishable
mg/kg mg/kg where Regulation 24 Table 1
applicable
Plastic/
Edible Fat minimum
Antioxidants Original NA
and Edible Oil 250g
Packaging
3 10 Non Room 2) CODEX STAN 192-1995
BHT
mg/kg mg/kg Perishable Temperature www.fao.org/gsfaonline/docs/CXS_192e.pdf
Aditif Makanan
Sauces,
Frozen/
Juices, minimum 1) AM 1983, PPM 1985, Sixth Sch, Regulation
2 30 Chilled
Benzoic Acid Preserved 500g Perishable 20 Table 1
mg/kg mg/kg where
Dried Fruit, (edible content/EN/ALL/?uri=CELEX%3A32011R0010
applicable
Chilli Slurry, portion
Fish & Fish without
Products, bones) Plastic/
Benzoic Acid & Pasta Soft for fish Original NA
Sorbic Acid Drink
8 30 and fish Non Room Packaging
Sorbic Acid products
mg/kg mg/kg Perishable Temperature
Other
5 20 food- 2) CODEX STAN 192-1995
Benzoic Acid Minimum Perishable
mg/kg mg/kg Room www.fao.org/gsfaonline/docs/CXS_192e.pdf
Flour, Bread 250 gm
6 20 Non Temperature
Sorbic Acid
mg/kg mg/kg Perishable
Cream, 1 5 3) EU Regulation http://eur-
Benzoic Acid Perishable Chilled
Coconut Milk mg/kg mg/kg lex.europa.eu/legal-
Page 212
Sample condition
LOD LOQ
Unit
weight
Perishable
1 5
Sorbic Acid
mg/kg mg/kg
MInimum Plastic/ 1) AM 1983, PPM 1985, Regulation 42

*Benzoyl Benzoyl 5 20 Non Room
Flour 250 gm/ Original NA 2) CODEX STAN 192-1995
Peroxide Peroxide mg/kg mg/kg Perishable Temperature
250ml Packaging www.fao.org/gsfaonline/docs/CXS_192e.pdf
Frozen/
Chilled
Perishable Plastic/
Pasta, Fish & 20 minimum where
Boric Acid Boric Acid NA applicable Original NA Prohibited in Food
Fish Product mg/kg 250g
Packaging
Non Room
minimum
500g
(edible 1) EU Regulation http://eur-
Frozen/
portion Plastic/ lex.europa.eu/legal-
Fish & Fish 5 25 Chilled
Citrate Citrate without Perishable Original NA content/EN/ALL/?uri=CELEX%3A32011R0010
Products mg/kg mg/kg where
bones) Packaging 2) CODEX STAN 192-1995
applicable
for fish www.fao.org/gsfaonline/docs/CXS_192e.pdf
and fish
products
C. The Comprehensive List of Food Division Tests in NPHL (Additive) Page 213
Sample condition
LOD LOQ
Unit
weight
Perishable
Frozen/
Chilled
Perishable
minimum where
500g applicable
Pasta : Pasta :
(edible 1) Administrative guideline BKKM PPI
0.8 2.5
portion Plastic/ 5/2006 & BKKM PPI6/2006 & BKKM PPI
All types of mg/kg mg/kg
Formaldehyde Formaldehyde without Original NA 3/2010 2) CODEX
food Fish : Fish :
bones) Packaging STAN 192-1995
20 20
for fish www.fao.org/gsfaonline/docs/CXS_192e.pdf
mg/kg mg/kg
and fish Non Room
products Perishable Temperature
6 20 minimum 1) AM 1983, PPM 1985, Regulation 147 &

*Nitrate 500g
mg/kg mg/kg 149
(edible
Frozen/
portion Plastic/
*Nitrate & Meat & Meat Chilled
without Perishable Original NA
Nitrite Products where
bones) Packaging
10 20 applicable 2) CODEX STAN 192-1995
*Nitrite for meat
mg/kg mg/kg and meat www.fao.org/gsfaonline/docs/CXS_192e.pdf
products
Sample condition
LOD LOQ
Unit
weight
Perishable
Frozen/
Chilled
Perishable
where
All types of MInimum applicable Plastic/ EU Regulation http://eur-
0.5 2
Phthalate DEHP food except 250 gm/ Original NA lex.europa.eu/legal-
mg/l mg/kg
oily food 250ml Packaging content/EN/ALL/?uri=CELEX%3A32011R0010
Non Room
125 125
Monophosphate minimum
mg/kg mg/kg
500g
125 125 (edible
Diphosphate Frozen/
mg/kg mg/kg portion Plastic/ EU Regulation http://eur-
Fish & Fish Chilled
Polyphosphate without Perishable Original NA lex.europa.eu/legal-
Products where
125 125 bones) Packaging content/EN/ALL/?uri=CELEX%3A32011R0010
Triphosphate applicable
mg/kg mg/kg for fish
and fish
375 375 products
Polyphosphate
mg/kg mg/kg
Bread and Plastic/

100 400 minimum Non Room
Propionic Acid Propionic Acid Flour Original NA AM 1983, PPM 1985, Regulation 65
mg/kg mg/kg 250g Perishable Temperature
Confectionery Packaging
Frozen/ Air tight 1) AM 1983, PPM 1985, Sixth Sch (Reg 20)
All types of 10 10 minimum Chilled container/ Table 1 2) CODEX
Sulfur Dioxide Sulfur Dioxide Perishable NA
food mg/kg mg/kg 250g where original STAN 192-1995
applicable packaging www.fao.org/gsfaonline/docs/CXS_192e.pdf
Sample condition
LOD LOQ
Unit
weight
Perishable
3) EU Regulation http://eur-
Non Room
lex.europa.eu/legal-
content/EN/ALL/?uri=CELEX%3A32011R0010
Solid Solid
matrix: matrix:
10 20
mg/kg mg/kg minimum
LIquid LIquid 250g
Sacharin matrix: matrix:
20 20
mg/kg mg/kg
Frozen/
1) AM 1983, PPM 1985, Seventeenth Sch,
0.01 0.2 minimum Chilled
Perishable Subregulation 133(2)Table 2, Regulation
mg/kg mg/kg 250g where
133(2B), Regulation 134
applicable
Solid Solid
matrix: matrix:
30 100 Plastic/
All types of
Sweeteners mg/kg mg/kg minimum Original NA
food
LIquid LIquid 250g Packaging
Cyclamate matrix: matrix:

80 120
mg/kg mg/kg
0.003 0.2 minimum

mg/kg mg/kg 250g
0.02 0.2 Non Room 2) CODEX STAN 192-1995

*Acesulfame K
mg/kg mg/kg Perishable Temperature www.fao.org/gsfaonline/docs/CXS_192e.pdf
0.004 0.2 minimum
*Aspartame
mg/kg mg/kg 250g
0.01 0.2
*Neotame
mg/kg mg/kg
Sample condition
LOD LOQ
Unit
weight
Perishable
0.01 0.2
*Sucralose
mg/kg mg/kg
7
Acid Red 1 mg/kg
(liquid)
2 mg/L
Auramine O
(liquid) Prohibited in Food
2 mg/L
Rhodamine B
(liquid)
2 mg/L
Patent Blue V
(liquid)
minimum
6
500g
Allura Red AC mg/kg Frozen/
(edible
(liquid) Solid Chilled
portion Perishable
5 matrix: where
without
Amaranth mg/kg 10 applicable
Synthetic bones) Plastic/
All types of (liquid) mg/kg
Water Soluble for meat Original NA
food 7 LIquid
Colour Brilliant Black and meat Packaging
mg/kg matrix:
PN products
(liquid) 20 1) AM 1983, PPM 1985, Seventh Sch,
Other
7 mg/kg Regulation 21 Table 1
Brilliant Blue food-
mg/kg
FCF Minimum
(liquid)
250 gm
4
Carmoisine mg/kg
(liquid)
7
Chocolate
mg/kg
Brown HT
(liquid) Non Room
5 Perishable Temperature
2) CODEX STAN 192-1995
Erythrosine BS mg/kg
www.fao.org/gsfaonline/docs/CXS_192e.pdf
(liquid)
Sample condition
LOD LOQ
Unit
weight
Perishable
6
Fast Green FCF mg/kg
(liquid)
6
Lissamine Green mg/kg
(liquid)
5
Ponceau 4R mg/kg
(liquid)
6
Quinoline
mg/kg
Yellow
(liquid)
4
Sunset Yellow
mg/kg
FCF
(liquid)
5
Tartrazine mg/kg
(liquid)
2 6
minimum
Para Red mg/kg mg/kg
500g
(spice) (spice)
(edible Frozen/
1 3
portion Chilled
Dimethyl Yellow mg/kg mg/kg Perishable
without where Plastic/
*Synthetic Oil All types of (spice) (spice)
bones) applicable Original NA Prohibited in Food
Soluble Colour food 2 6
for meat Packaging
Sudan 1 mg/kg mg/kg
and meat
(spice) (spice)
products
4 15
Other Non Room
Sudan 2 mg/kg mg/kg
food- Perishable Temperature
(spice) (spice)
Sample condition
LOD LOQ
Unit
weight
Perishable
4 15 Minimum
Sudan 3 mg/kg mg/kg 250 gm
(spice) (spice)
1 3
Sudan 4 mg/kg mg/kg
(spice) (spice)
using larvae F1 and adults F1 as samples, accordingly.
Page 220
Eggs are reared until adults to produce progeny, F1

(from eggs to larvae (L3) F1, takes about 45 days).

albopictus). Tests are done separately by species

larvae/female mosquitoes of the same species).

differentiate species (Aedes aegypti & Aedes

Test will proceed with enough samples (140

Identification is made from larva phase to

Results can be obtained by email or

Remarks
manually/postage.
manually/postage.
Initiated by Vector Borne Disease Section, Initiated by Vector Borne Disease Section,
Important Notes
Disease Department, Putrajaya Disease Department, Putrajaya
Tel No: 03-
1284, 2223 1284, 2223
61261200 Ext No.
Laboratory Name Entomology & Pest Entomology & Pest
Cost/Test (RM) RM 13.85 RM 19.28
Frequency of Test Daily Daily
Laboratory Turn-
Around-Time Female adults F0 = 10 days per insecticide,
larvae F1 = 35 days, F2 = 65 days
(LTAT) working F1 = 40 days, F2 = 70 days
days
D. The Comprehensive List of Entomology Tests in NPHL
Type of form KKM/BPS/001/2014, Data Kajian Ovitrap KKM/BPS/001/2014, Data Kajian Ovitrap
Sample Biohazard Zip Lock plastic bag place in a Biohazard Zip Lock plastic bag place in a
Transportation clean container clean container
Collection Ovitrap in field for 14 days Ovitrap in field for 14 days
method
Container/ “Paddle” –hard board (approximate size 1 “Paddle” –hard board (approximate size 1 “
Transport media “ x 6 “) 200 pieces and above x 6 “) 200 pieces and above
Sample Volume More than 1000 eggs More than 1000 eggs
Sample type Eggs Eggs
WHO Insecticide Resistance Test on WHO Insecticide Resistance Test on
Test Method Mosquitoes of Health Importance : Mosquitoes of Health Importance : alpha-
temephos cypermethrin
Category Surveillance Surveillance
Patogen/ Vector Aedes spp. (larva) Aedes spp. (adult)
Page 221







Remarks
manually/postage.
manually/postage.
Initiated by Vector Borne Disease Section, Initiated by Vector Borne Disease Section, Disease
Important Notes
Disease Department, Putrajaya Department, Putrajaya
Tel No: 03-
1284, 2223 1284, 2223
61261200 Ext No.
Laboratory Turn-
Around-Time Female adults F0 = 10 days per Female adults F0 = 10 days per insecticide, F1 =
(LTAT) working insecticide, F1 = 40 days, F2 = 70 days 40 days, F2 = 70 days
days
Sample Biohazard Zip Lock plastic bag place in a Biohazard Zip Lock plastic bag place in a clean
Transportation clean container container
method

Container/ “Paddle” –hard board (approximate size 1 “Paddle” –hard board (approximate size 1 “ x 6 “)
Transport media “ x 6 “) 200 pieces and above 200 pieces and above
WHO Insecticide Resistance Test on
WHO Insecticide Resistance Test on Mosquitoes
Test Method Mosquitoes of Health Importance :
of Health Importance : deltamethrin
cyfluthrin
Patogen/ Vector Aedes spp. (adult) Aedes spp. (adult)
Page 222



species).Results can be obtained by email or


larvae/female mosquitoes of the same

Remarks
manually/postage.
manually/postage.
Important Notes Disease Department, Putrajaya Disease Department, Putrajaya
Tel No: 03- 1284, 2223 1284, 2223
61261200 Ext No.
Entomology & Pest Entomology & Pest
Laboratory Name
RM 19.28 RM 19.28
Cost/Test (RM)
Daily Daily
Frequency of Test
Laboratory Turn- Female adults F0 = 10 days per insecticide, Female adults F0 = 10 days per insecticide, F1
Around-Time F1 = 40 days, F2 = 70 days = 40 days, F2 = 70 days
(LTAT) working
days
KKM/BPS/001/2014, Data Kajian Ovitrap KKM/BPS/001/2014, Data Kajian Ovitrap
Type of form
method

Container/ “Paddle” –hard board (approximate size 1 “ “Paddle” –hard board (approximate size 1 “ x 6
Transport media x 6 “) 200 pieces and above “) 200 pieces and above
More than 1000 eggs More than 1000 eggs
Sample Volume
Test Method Mosquitoes of Health Importance : Mosquitoes of Health Importance : lambda-
etofenprox cyhalothrin
Page 223








Remarks
manually/postage.
manually/postage.
Important Notes
Tel No: 03-
1284, 2223 1284, 2223
61261200 Ext No.
Laboratory Turn-
Around-Time Female adults F0 = 10 days per Female adults F0 = 10 days per insecticide, F1 =
(LTAT) working insecticide, F1 = 40 days, F2 = 70 days 40 days, F2 = 70 days
days
method

Container/ “Paddle” –hard board (approximate size 1 “Paddle” –hard board (approximate size 1 “ x 6
Transport media “ x 6 “) 200 pieces and above “) 200 pieces and above
of Health Importance : fenitrothion
permethrin
Page 224








Remarks
manually/postage.
manually/postage.
Important Notes Disease Department, Putrajaya Disease Department, Putrajaya
Tel No: 03- 1284, 2223 1284, 2223
61261200 Ext No.
Entomology & Pest Entomology & Pest
Laboratory Name
RM 19.28 RM 19.28
Cost/Test (RM)
Daily Daily
Frequency of Test
Laboratory Turn- Female adults F0 = 10 days per insecticide, Female adults F0 = 10 days per insecticide, F1
Around-Time F1 = 40 days, F2 = 70 days = 40 days, F2 = 70 days
(LTAT) working
days
KKM/BPS/001/2014, Data Kajian Ovitrap KKM/BPS/001/2014, Data Kajian Ovitrap
Type of form
method

Container/ “Paddle” –hard board (approximate size 1 “ x “Paddle” –hard board (approximate size 1 “ x
Transport media 6 “) 200 pieces and above 6 “) 200 pieces and above
More than 1000 eggs More than 1000 eggs
Sample Volume
Test Method Mosquitoes of Health Importance : Mosquitoes of Health Importance : pirimiphos
malathion methyl
Page 225
Relative humidity of 70%± 10% can be sustained by
placing ice packs in the container.

Remarks
manually/postage.
Important Notes
Tel No: 03-
1284, 2223 1284, 2223
61261200 Ext No.
Frequency of Test Twice per year/ upon request Twice per year/ upon request
Laboratory Turn-
Around-Time 5 days 5 days
(LTAT) working
days
Type of form KKM/BPS/001 KKM/BPS/001
Sample Universal bottle placed in a cool box Any big container with preserved relative
Transportation humidity (70%± 10%)
Collection Dipping method Human Landing Catch (HLC), Animal-baited
method trap, Resting collection, Human-baited trap

Container/
Universal bottle Mosquito cage
Transport media
Sample Volume At least 140 larvae (alive specimens) A least 140 female adults
Sample type Field collection Wild-caught females
of Health Importance : deltamethrin
temephos
Patogen/ Vector Anopheles spp. (larva) Anopheles spp. (adult)
Page 226
Relative humidity of 70%± 10% can be sustained by
placing ice packs in the container. Results can be
obtained by email or manually/postage.

Remarks
manually/postage.
Important Notes
Tel No: 03-
1284, 2223 1284, 2223
61261200 Ext No.
Cost/Test (RM) RM 19.28 RM 100
Frequency of Test Twice per year/ upon request Monthly
Laboratory Turn-
(LTAT) working
days
Sample Any big container with preserved relative Eppendorf tube placed in a Biohazard Zip Lock
Transportation humidity (70%± 10%) plastic bag
Collection Human Landing Catch (HLC), Animal-baited Human Landing Catch (HLC), Animal-baited
method trap, Resting collection, Human-baited trap trap, Resting collection, Human-baited trap

Container/
Mosquito cage Eppendorf tube
Transport media
Sample Volume A least 140 female adults 20-25 female adults
Sample type Wild-caught females Wild-caught females
Test Method Mosquitoes of Health Importance : Identification by morphology
permethrin
Patogen/ Vector Anopheles spp. (adult) Mansonia spp.
Page 227

Remarks
manually/postage.
manually/postage.
Important Notes
Tel No: 03-
1284, 2223 1284, 2223
61261200 Ext No.
Cost/Test (RM) RM 100 RM 100
Frequency of Test Monthly Monthly
Laboratory Turn-
(LTAT) working
days
Sample Eppendorf tube placed in a Biohazard Zip Eppendorf tube placed in a Biohazard Zip Lock
Transportation Lock plastic bag plastic bag

Container/
Eppendorf tube Eppendorf tube
Transport media
Sample Volume 20-25 female adults 20-25 female adults
Test Method Identification by morphology Identification by morphology
Patogen/ Vector Coquillettidia crassipes Culex quinquefasciatus
Page 228

Remarks
manually/postage.
manually/postage.
Important Notes
Tel No: 03-
1284, 2223 1284, 2223
61261200 Ext No.
Cost/Test (RM) RM 100 RM 100
Frequency of Test Monthly Monthly
Laboratory Turn-
(LTAT) working
days
Sample Eppendorf tube placed in a Biohazard Zip Eppendorf tube placed in a Biohazard Zip Lock
Transportation Lock plastic bag plastic bag

Container/
Eppendorf tube Eppendorf tube
Transport media
Sample Volume 20-25 female adults 20-25 female adults
Patogen/ Vector Anopheles spp. Armigeres subalbatus
Page 229
Eggs are reared until adults to produce progeny, F1.
albopictus). Results can be obtained by email or

Identification are made from larva phase to
Remarks
manually/postage.
manually/postage
Initiated by Vector Borne Disease Section, Colonization done to assist State Vector
Important Notes
Disease Department, Putrajaya without Insectarium
Tel No: 03-
1284, 2223 1284, 2223
61261200 Ext No.
Cost/Test (RM) 100 100
Frequency of Test Monthly Upon request
Laboratory Turn-
Around-Time 14 days Female adults F1 = 40 days, F2 = 70 days
(LTAT) working
days
Type of form KKM/BPS/007 KKM/BPS/001/2014, Data Kajian Ovitrap
Sample Eppendorf tube placed in a Biohazard Zip Lock Biohazard Zip Lock plastic bag place in a
Transportation plastic bag clean container
Collection Human Landing Catch (HLC), Animal-baited Ovitrap

Container/ “Paddle” –hard board (approximate size 1 “
Eppendorf tube
Transport media x 6 “)
Sample Volume 20-25 female adults More than 1000 eggs
Sample type Wild-caught females Eggs
Category Surveillance Colonization
Patogen/ Vector Aedes spp. Aedes spp.
Page 230
Remarks
manually/postage.
Initiated by Vector Borne Disease Section,

Important Notes
Disease Department, Putrajaya
Tel No: 03-
1284, 2224
61261200 Ext No.
Laboratory Name Entomology & Pest
Cost/Test (RM) 100
Frequency of Test Upon request
Laboratory Turn-
Around-Time 7 days
(LTAT) working
days
Type of form KKM/BPS/001/2014
Sample Paper cup with netting. In a cool
Transportation environment.
Collection Human Landing Catch (HLC), Animal-baited

Container/
Paper cup with netting.
Transport media
Sample Volume 20-25 female adults
Sample type Wild-caught females
Test Method Identification by morphology
Category Surveillance
Patogen/ Vector Aedes sp.
SPECIMEN COLLECTION PROCEDURES FOR DISEASE DIVISION
LABORATORY TESTING
Proper collection and transportation of appropriate specimen plays an important

role in obtaining accurate laboratory diagnosis. The specimens must be collected at
the right time using the right procedures and transported to the NPHL in the right
way.
A. CLINICAL SPECIMEN
1. GENERAL RULES
1.1. Aseptic technique must be practiced throughout the procedure.
1.2. Collect the specimen at the right phase of the disease. For microbiological testing, collect the
specimen before antibiotic therapy.
1.3. Collect sufficient specimen volume/quantity.
1.4. Transfer the specimen aseptically in the correct sterile container or transport medium.
1.5. Close the container tightly and seal with parafilm if required to avoid leakage during
transportation.
1.6. Each specimen and requisition form must be labeled properly and completely.
1.7. Place the specimen container inside a biohazard bag. Each sample is to be packed in separate
biohazard bags.
1.8. Pack the specimen properly and send to laboratory as soon as possible. (Please refer to the A
VISUAL GUIDE FOR INFECTIOUS DISEASE SAMPLE COLLECTION).
1.9. Maintain the correct temperature from specimen collection until arrival at NPHL. Refer to The
Comprehensive List of Infectious Disease Tests in NPHL for the correct temperature ranges.
2. SERUM
2.1. Draw 3-5ml blood into a plain tube with/without gel (No anticoagulants or preservatives).
2.2. Allow to clot at ambient temperature for a minimum of 10 minutes.
2.3. Centrifuge at 3,000 rpm for 10 minutes.
2.4. Aliquot the serum into another transfer tube when using a tube without gel.
2.5. For a tube with gel, please send the original tube.
2.6. Seal the tube with parafilm prior to transportation.
3. RESPIRATORY SPECIMEN
3.1. Throat Swab
3.1.1. Depress the tongue with a tongue depressor.
3.1.2. Swab the inflamed area of the throat, pharynx or tonsils with a sterile swab taking care
to collect the pus or piece of membrane. Avoid touching the cheeks, tongue, uvula or
lips.
3.1.3. Place the swab for Bacteriology testing in Amies transport medium (with or without
charcoal).
3.1.4. Place the swab for Virology testing in Viral Transport Media (VTM).
3.2. Nasopharyngeal Swab
Page 231
3.2.1. Use only Dacron or polyester swabs.
3.2.2. Hold back the patient’s head slightly and insert the swab straight into the nostril.
3.2.3. Insert the swab to least 5-6cm in length (for adults) to ensure that it reaches the
posterior pharynx.
3.2.4. Leave the swab in place for few seconds, rotate and withdraw slowly.
3.2.5. Repeat the same procedure on the other nostril using new swab.
3.2.6. For Bacteriology Culture and Sensitivity testing, place the swab in Amies transport
medium with charcoal or without charcoal. The sample shall be transported at ambient
temperature.
3.2.7. For Bordetella pertussis Genome Detection testing, place swab in sterile container with
sterile saline or Amies Transport Medium (without charcoal). The sample shall be
transported at 2-8C.
3.2.8. For Virology testing place swab in Viral Transport Media (VTM). The sample shall be
transported at 2-8C.
3.3. Nasopharyngeal Aspirates (NPA)/Nasopharyngeal Secretion

3.3.1. Insert a small catheter through the nares to the back of nose.
3.3.2. Gently suction as the catheter is withdrawn slowly.
3.3.3. Collect in sterile screw capped container.
3.3.4. Ensure that the container is sealed securely to prevent leakage.
3.3.5. The sample shall be transported at 2-8C (for both Bacteriology and Virology testing).
3.4. Sputum
3.4.1. Collect the sputum early in the morning.
3.4.2. Use a wide-mouthed container for collection.
3.4.3. Instruct the patient to inhale deeply 2-3 times, cough up deeply from the chest and
spit the sputum into the container by bringing it closer to the mouth.
3.4.4. Make sure the sputum sample is of good quality (2-3ml of thick and purulent sputum).
4. BODY FLUIDS
4.1 Collect the specimen (pleural, pericardial, peritoneal, synovial, amniotic fluid, etc.) according
to the Clinical Practice Guidelines (CPG) and transfer 2 to 5ml specimen into a sterile screw
capped container.
4.2 Cerebrospinal Fluid (CSF)

4.2.1 Collect CSF before antimicrobial therapy is started.
4.2.2 Collect 1-3ml in sterile screw capped container (Bijou bottle or Cryo vial).
4.2.3 Do not use urine container.
4.3 Blood Cultures and Bone Marrow Aspirate for TB

4.3.1 Fill 5-10 ml of blood into Myco F Lytic bottle by using aseptic technique.
4.3.2 Send the specimens to the laboratory as soon as possible or incubate them at 370C
in an incubator if there is a delay in transit.
4.3.3 Do not store in the refrigerator.
4.3.4 For bone marrow aspirate, aspirate 1-2 ml and inoculated directly into the bottles.
4.3.5 Send the specimen to the laboratory as soon as possible.
Specimen Collection Procedures For Disease Division Laboratory Testing (A. Clinical Specimen) Page 232
5. URINE AND STOOL/RECTAL SWAB
5.1. Urine
5.1.1.Adult Male
5.1.1.1. Give the patient a sterile urine container.
5.1.1.2. Instruct the patient to wash hands with soap and water before collection of
urine specimen.
5.1.1.3. Cleanse the glands penis with soapy water and rinse with water.
5.1.1.4. Pass the few millimetres of urine to flush out the bacteria from the urethra,
and then collect the mid-stream urine (MSU) in sterile urine container.
5.1.2. Adult Female

5.1.2.1. Give the patient a sterile urine container.
5.1.2.2. Instruct the patient to wash hands with soap and water before collection of
urine specimen.
5.1.2.3. Cleanse the area around the urethral opening with clean water, dry the area,
and collect the urine with the labia held apart.
5.1.2.4. Discard the first portion of the stream and collect MSU in sterile urine
container (without preservative).
5.1.3. Infant and young children

5.1.3.1. Instruct the child to drink water.
5.1.3.2. Clean the external genitalia.
5.1.3.3. Encourage the child to urinate and collect the MSU in sterile urine container.
* Urine collection bag can be used to collect urine.

* If is not possible to send the urine specimen to NPHL within 2-4 hours, boric acid must
be added, except for virology testing.
5.2. Stool/Rectal Swab

5.2.1. Collect 5g or 5ml stool into stool screw-capped container.
5.2.2. Collect rectal swab if fresh stool collection is not possible. Ensure that the swab shows
some faecal staining.
5.2.3. Place the swab for Bacteriology testing in Amies transport medium (with or without
charcoal).
5.2.4. Place the swab for Virology testing in Viral Transport Media (VTM).
6. EYE/EAR/GENITAL/PUS SWAB
6.1. Eye swab
6.1.1.Clean skin around the eye using a sterile moistened swab to remove pus and discharge.
6.1.2.Use a separate swab for each eye for specimen collection.
6.1.3.Place the swab for Bacteriology testing in Amies transport medium (with or without
charcoal).
6.1.4.Place the swab for Virology testing in Viral Transport Media (VTM).
6.2. Ear Swab

6.2.1.Do not apply any antibiotic drops 3 hours prior to specimen collection.
6.2.2.Swab the external ear canal by using a sterile swab.
6.2.3.Place the swab for Bacteriology testing in Amies transport medium (with or without
charcoal).
6.2.4.Place the swab for Virology testing in Viral Transport Media (VTM).
6.3. Urethral Discharge Swab for Sexually Transmitted Infection (Male)
6.3.1.Clean the foreskin of the penis using sterile moistened swab.
6.3.2.Collect the exudates with a sterile swab and inoculate into the Amies transport medium
with/without charcoal or Stuart transport medium.
6.3.3.If discharge cannot be obtained, use a sterile swab to collect material from about 2cm
inside the urethra.
6.4. Pus/Pus Swab

6.4.1.Clean the skin around the specimen collection area using a sterile swab.
6.4.2.Collect pus in sterile screw capped container or if minimal pus is available, use a sterile
swab. Place the swab in Amies transport media with/without charcoal or Stuart
Transport Media.
7. SKIN BIOPSY/LESIONS/VESICLE SWABS

7.1. Skin Biopsy for Leprosy
7.1.1.Take a minimum size of 4mm x 12mm skin biopsy samples or a minimum of 5mm punch
biopsy.
7.1.2.Place the samples into sterile screw capped container without preservatives for mouse
foot pad inoculation for drug sensitivity testing.
7.1.3.Samples for detection of Mycobacterium leprae using PCR method and detection of
drug resistant Mycobacterium leprae by Molecular Line Probe Assay Method can be
transported in sterile screw capped container without preservatives or with 70%
ethanol.
7.2. Skin lesions/Vesicle swab

7.2.1.Examine the body part and choose the largest vesicle (representative lesion).
7.2.2.Clean the area around the lesion gently with a normal saline-soaked cotton swab.
7.2.3.Rupture the vesicle carefully with a hypodermic needle.
7.2.4.Swab the vesicular fluid from the ruptured vesicle quickly or use the swab to squeeze
out the vesicular fluid.
7.2.5.Place the swab in VTM.
7.2.6.Send the specimen to laboratory as soon as possible.
B. ENVIRONMENTAL AND NON CLINICAL SPECIMEN
1.0 UNTREATED WATER SAMPLING FOR LEPTOSPIRA

1.1. Select appropriate water sampling areas, such as:
1.1.1. Bodies of water such as lakes/ wells/ rivers/ waterfalls etc.
1.1.2. Water in a shaded area.
1.1.3. Water in an area with the presence of animals (i.e. paw print marks).
1.1.4. Water in between stones or cracks on the stone.
1.1.5. Water samples taken one foot below the water surface.
1.2. Record temperature and pH (compulsory) in the test request form.

1.3. Sterilize the collection bucket/pail by pouring 70% alcohol. Let it dry.
1.4. Lower the bucket into the river/ well/ pond/ lake and ensure that the connecting rope/string
does not touch inner part of the bucket.
1.5. Draw up bucket with caution when it is full.
1.6. Fill 250ml (minimum) of the collected untreated water into sterile universal samples bag (e.g.
Whirl-Pak bags).
1.7. Label accordingly and put it into the cooler box following a vertical arrangement.
1.8. Wash the bucket with clean water after use, sterilize with 70% ethanol and dry it.
1.9. Repeat procedures for sampling in other areas.
2.0 SOIL SAMPLING FOR LEPTOSPIRA

2.1 Select the land area for sampling with distance of less than 5 meters from a river/pond/ lake.
2.2 The appropriate soil sampling areas are:
2.2.1 Wet land area soil.
2.2.2 Shaded area soil.
2.2.3 Soil in an area with presence of animals (ie: paw print marks).
2.3 Sterilize the respective tools by pouring 70% alcohol. Let it dry.
2.4 A soil sample size of 15 – 20 cm X 4 – 8cm should be taken in the area after all the loose
surface materials are removed.
15 – 20 cm
4 – 8 cm
2.5 Place 200 g (minimum) of soil into a sterile universal sample bag (e.g. Whirl-Pak).
2.6 Record temperature and pH in the test request form.
3.0 WATER SAMPLING FOR LEGIONELLA

3.1 Collect water from a central air conditioning cooling tower or other similar types of water
sources.
3.1.1 Water samples must be collected before dosing with biocide.
3.1.2 If dosing has been carried out, take water samples at least 3 days after the dosing.
3.2 Ensure that the central air conditioning system is operating and water from the cooling tower
is circulated through the system for at least one hour before collection of samples.
3.3 Do not collect samples near the take-up water inlet.
3.4 Do not stir up sediments in cooling tower.
3.5 Take 1000 ml of water for each sample in sterile containers.
*Note: Please notify the laboratory at least 3 days before sending the samples.
Page 235
4.0 SOIL SAMPLING FOR BURKHOLDERIA PSEUDOMALLEI
4.1 Use a spade, a small gardening shovel or a scoop.
4.2 Sterilize the tools by pouring 70% alcohol. Let it dry.
4.3 Collect a 100g (minimum) of moist soil samples from a depth of 30cm during the dry season or
surface soil during the rainy season.
4.4 Put the soil sample in a sterile universal sample bag (e.g. Whirl-Pak).
4.5 Label the sample accordingly and record the site sampled.
5.0 WATER SAMPLE FOR BURKHOLDERIA PSEUDOMALLEI

5.1 Sterilize the tools by pouring 70% alcohol. Let it dry.
5.2 Collect 100ml of stagnant water (from a suspected contaminated pool of water) into a sterile
universal sample bag.
5.3 Label the sample accordingly and record the site sampled.
6.0 CIGARETTES TESTING (NICOTINE, TAR & CARBON MONOXIDE)

The procedure for sample collection is regulated under Regulation 17 Control of Tobacco Product
Regulations 2004 and Section 5 of the Food Act 1983.
Specimen Collection Procedures (B. Environmental & Non Clinical Specimen) Page 236
C. SPECIMEN REJECTION CRITERIA
The specimens will be rejected if
1.1 Request Form

1.1.1 Request form without sample
1.1.2 No IC, name or R/N of patient
1.1.3 No address of requestor
1.1.4 No signature of requesting doctors or requestors.
1.1.5 No test indicated
1.1.6 Date of collection is not stated
1.1.7 Incomplete required clinical history
1.2 Sample
1.2.1 Sample without request form
1.2.2 No label
1.2.3 Leaking sample
1.2.4 Haemolysed sample
1.2.5 Insufficient sample
1.2.6 The type of specimen sent is not appropriate for the test requested.
1.2.7 Wrong container
1.3 Others
1.3.1 Test is not offered
1.3.2 Specimen received is not in suitable condition.
Page 237
A VISUAL GUIDE FOR INFECTIOUS DISEASE SAMPLE COLLECTION
The steps Visual guide

Select the correct collection transport
container and medium by referring to the
A. The Comprehensive Directory of
Infectious Disease Tests in NPHL before
commencing collection procedure. Ensure
that the transport medium used is not Flocked swab
expired.
Swab with Cary Blair medium
Swab with Amies (without charcoal) medium
Swab with Amies (with charcoal) medium
Place the collected sample in sterile

container or VTM [for throat or
nasopharyngeal swab (NPS)]. Sample in sterile
Bijoux bottle
Throat /
NPS swab in
VTM
Page 238
Label the sample with:
1. Patient’s name;
2. Patient’s ID number;
3. Sample type; The label
4. Date of sample collection.
Fill in the laboratory request form with Refer to the Appendix.

complete details.
Place individual samples in separate

biohazard plastic bags.
1 sample /
plastic bag
Follow the proper triple packing guidelines

especially when packing highly infectious
substances/samples to ensure safe
transportation.
A Visual Guide For Infectious Disease Sample Collection Page 239

Put the sample into cooler box with 3 units
of ice packs (i.e. 1 unit at the bottom of the
cool box and 2 units at both sides of the Ice packs
samples). Place some absorbent material
to protect the container from high impact
or unforeseen rough handling of the cooler
box. Place sample
individually
Seal the cool box.

Laboratory
request form
Place the laboratory request form in a
plastic bag and paste on top of the cool
box. Ensure that the accompanying request
form is secured properly to the box to
prevent loss or misplacement of request
forms. Seal the cool
box
Transport the sample to the respective
regional public health laboratories at 2 to
8°C within 48 hours after collection.
Address of MKA
All samples must be sent to the sample
receiving counter of the respective
laboratories.
A Visual Guide For Infectious Disease Sample Collection Page 240

SPECIMEN COLLECTION PROCEDURES FOR FOOD DIVISION
LABORATORY TESTING
A. SAMPLING PROCEDURE FOR MICROBIOLOGICAL ANALYSIS
1. UTENSILS AND STERILIZATION

1.1. Sampling should be done quickly and with caution.
1.2. Make sure that the staffs involved are not wearing rings,
watches, bracelets, etc.
1.3. Wear the suitable personal protective equipment (PPE)
including masks and sterile, powder-free gloves.
1.4. Wear an overall/apron first, followed by, a mask and a cap.
1.5. Follow the 7 hand washing steps before sampling is carried
out (http://fsq.moh.gov.my/v5/ms/faq-cara-membasuh-
tangan-yang-betul/).
1.6. The seven hand washing steps must also be done if cross
contamination occurs or is identified during sampling
activities.
1.7. Wipe hands by using hand sanitising wipes or a hand
sanitizer that contains at least 75% alcohols.
1.8. Wear sterile gloves.
1.9. Perform the sampling aseptically by using the appropriate
sterile utensils such as sterile spoons, forceps or ladles to
prevent cross contamination. If possible, use the food
handler’s utensils which were used for serving food to
customers.
1.10. Metal and stainless steel utensils can be sterilized directly by
using a propane torch, spirit lamp or other suitable
apparatus.
2. PROCEDURE FOR USING SAMPLING BAGS

2.1. Ensure that the sterile sampling bag is opened only a moment before placing the food
samples into the bag and close the sampling bag immediately after.
2.2. Do not touch the inner part and the opening area of the sampling bags.
2.3. Label the bag with sample information.
Page 241
2.4. Tear off the top of the bag along the perforation.
2.5. Use pull tabs on each side to open the bag. Sometimes a little pull on the bottom of the
bag helps open it completely.
2.6. Put sample, liquid or solid, into the bag. Make sure that the ready-to-eat (RTE) sample is
not over-filled in the sampling bags. Leave enough space at the top for closing and mixing
if needed.
2.7. Pull the ends of the wire to close the bag. Holding the bag by the wire ends, whirl the bag
three complete revolutions to form a leak-proof seal. Whirling the bag will form the tightest
seal. Larger bags can be closed by “folding” the tab over as tightly as possible. Bend the wire
ends over onto the bag to complete the closing. Make sure no air is trapped in the sampling
bags.
A. Sampling Procedure For Microbiological Analysis Page 242

3. QUALITY CONTROL OF SAMPLE DURING SAMPLING
3.1. For the positive control of sampling bags during sampling activities, make sure that the time
exposed to the air for the sampling bag is same to the time used for the sampling activities.
Sterile distilled water can be filled into the positive control of sampling bags. It can indicate
the air quality at the sampling area.
3.2. Un-opened sampling bags can be used as negative control of sampling bag during sampling
activities.
4. SWAB SAMPLING PROCEDURE

4.1. The suitable type of swab to be used for
monitoring of environmental hygiene are 3M
quick swab, Hygiena Q-swab and etc.
4.2. For wet swabbing,
4.2.1.Label the swab, bend snap valve at a 45°
angle until snapping sound is heard.
4.2.2.Squeeze bulb to transfer all the broth to
the tube.
4.2.3.Remove swab from the tube, and swab
targeted area. Place swab back into tube
and bring to the lab.
4.2.4.Shake tube vigorously for 10 seconds to
release bacteria from swab.
4.3. For dry swabbing,
4.3.1.Label the swab, remove swab from the tube, and swab targeted area. Place swab back
into tube and bring to the lab.
4.3.2.Bend snap valve at a 45° angle until snapping sound is heard. Squeeze bulb to transfer
all the broth to the tube.
4.3.3.Shake tube vigorously for 10 seconds to release bacteria from swab.

5. LABELLING AND ‘LAK’ PROCEDURE
5.1. Insert the label in the small plastic packaging and ‘lak’ the sample bags accordingly.
5.2. Take the photos of the sample for further reference (court case).
5.3. Insert the labelled sample bags into second layer of transparent plastic packaging to protect
the label and ‘lak’.

6. PROCEDURE FOR PACKING OF SAMPLES IN AN ICE BOX
6.1. The samples are separated in crushed ice / ice cube, no hot spot occurred, enhanced rapid
cooling and easy flowing for melting ice.
6.2. Sample will be rejected by the laboratory if hot spot occurred. Hot spot can occur due to:-
6.2.1.The size of ice is too big and not enough to control the temperature inside the box
6.2.2.The overlapping samples
6.2.3.There are too many samples
6.2.4.Sample touch to an ice box
6.2.5.The big air space in packaging
6.2.6.Using ice pack

B. SAMPLING PROCEDURE FOR CHEMICAL ANALYSIS
1. GENERAL RULES
1.1. Refer PART III PROCEDURE FOR TAKING SAMPLES, Regulation 4 in Food Regulations 1985.
1.2. An authorized officer shall :–
1.2.1. divide the sample into three separate parts and mark and seal or fasten up each part
in such a manner as its nature will permit.
1.2.2. offer one part to the seller, importer or manufacturer or his agent or the person
having charge of the food;
1.2.3. deliver either personally or through another authorized officer or by A.R.
(Acknowledgement of Receipt) registered mail or by courier services with
acknowledgment of receipt one of the remaining parts to an analyst; and
1.2.4. retain the other remaining part.
1.3. Label the food sample according to Regulation 6 in Food Regulations 1985.
1.3.1. The label for food sample shall be in quadruplicate with a common counterfoil in the
form as prescribed in the Second Schedule in Food Act 1983.
1.3.2. Where a food sample is divided into three parts one of such label shall be pasted on
each part of the sample while the remaining label is to be affixed to the request for
analysis form.
1.3.3. Sample need to be paste with official lacquer (lak rasmi).
1.4. The request for analysis of food sample shall be made in Form A as set out in the Third
Schedule in Food Act 1983.
1.5. Obtain a sample acceptance letter or receipt from analyst.
2. SPECIAL REQUIREMENT OF SAMPLING FOR PESTICIDES ANALYSIS

2.1. For pesticides analysis; sample like cabbage, watermelon must not be divided (cannot be cut)
as it might affect the composition or impede the proper analysis of the content.
2.2. For samples such as fruits & vegetables, it is recommended to wrap it using clean browning
paper to avoid it from wilting. Other options use clean containers and robust packaging such
as Polythene or polypropylene bags, ventilated if appropriate. Perishable sample such as fish
and meat are wrapped using clean packaging with no ventilated hole.
2.3. It is also recommended to submit as soon as possible to the laboratory preferably within one
day, especially fresh products as it (pesticides) can be degraded.
2.4. Samples of commodities pre-packed for retail sale should not be removed from their
packaging before transport.
2.5. Samples that are frozen at the time of collection must be transported without thawing.
Samples that may be damaged by chilling (e.g. bananas) must be protected from both high
and low temperatures.
2.6. The use of marker pens containing organic solvents should be avoided for labelling bags
containing samples to be analysed for fumigant residues, especially if an electron capture
detector is to be used.
3. SPECIAL REQUIREMENT OF SAMPLING FOR HEAVY METAL ANALYSIS

3.1. Any samples for heavy metals analysis should not have direct contact with any metals
container or apparatus.
Page 246
4. SPECIAL REQUIREMENT OF SAMPLING FOR VETERINARY DRUG RESIDUE ANALYSIS
4.1. Sample should be sent to the laboratory as soon as possible, after taking precaution against
leakage and spoilage
4.2. For shipping/ courier: if possible all perishable samples should be frozen to minus 20⁰C,
immediately after collection
4.3. Quantity needed do not include the packaging or inedible portion.
5. SPECIAL REQUIREMENT OF SAMPLING FOR STANDARD AND NUTRITIONAL LABELLING

ANALYSIS
5.1. Sample should send to laboratory according to the requirements in the 1983 Food Act under
PART VIII Standards and Particular Labelling Requirement for Food. This is referring to
Regulation 42 until Regulation 395.
5.2. Example:
5.2.1. Parameter being requested: Percent of Fat
5.2.2. Regulation 62: Oatmeal shall contain not less than 5% of oat fat.
5.2.3. Example of food sample:
5.2.4. Oatmeal (cleaned oats after the removal of the husk and not mixed with other
ingredients)
5.3. If mixed with other substances such as sugar, malt and salt, it falls under Regulation 64:
Prepared Cereal Food. Under this regulation, no allowable percentage of fat content is
specified.
5.4. Any request for testing of Food Standard shall be referred to Food Regulations 42 to 395 for
its suitability prior to sampling.
B. Sampling Procedure For Chemical Analysis Page 247

FOOD DIVISION CLIENT CHARTER
SERVICES SAMPLE TYPE LABORATORY TURN AROUND TIME

(DAYS)
1 OUTBREAK / CRISIS (Food poisoning, complaint, etc.)
i Chemical Analysis All food 7

ii Microbiological Analysis All food, environmental and hand swab 4 - 18
iii Molecular Analysis All food 7
iv GMO & DNA Analysis All food 7
2 ENFORCEMENT (Routine/Domestic, Operational, etc.)

ii Microbiological Analysis All food, environmental and hand swab 18
21 (Commercial sterility test)

3 SURVEILLANCE / MONITORING / PROGRAMME / RESEARCH
3.1) Plan, Ad-hoc, #TDS, National / Specific Research

ii Microbiological Analysis All food, environmental and hand swab
iii Molecular Analysis All food
iv GMO & DNA Analysis All food
Page 248
(DAYS)
3.2) *Export

3 3.3) Import
a) Hold Test Release / Undertaking


b) Entry Point Monitoring

21 (Commercial sterility test)

3.4) Pre-Market Approval and Industry
Food Division Client Charter Page 249

(DAYS)

3.5) **Subcontract

Food Division Client Charter Page 250

NPHL OPERATING HOURS AND ON CALL CONTACT NUMBER
Normal Service Hours - From 7.30am to 5.30pm

Mondays to Fridays excluding Public Holidays.
*All samples/specimens should be sent and received by MKAK during the normal service hours except
for outbreak samples/specimens.
Outbreak Service Hours (24-hour service)

*Please visit MKAK web site http://mkak.moh.gov.my/ms/ (Muat Turun> Jadual On-Call> Bahagian
Penyakit> Jadual On Call> Bahagian Penyakit or Bahagian Makanan) for the current on-call personnel
in-charge.
Customers should inform the on-call personnel prior sending the samples/specimens by calling the
NPHL Hotline at +6011-1438 2816.
RESULTS TRACING
A. SIMKA (Sistem Informasi Maklumat Kesihatan Awam)
Results for samples sent to the Disease Division sections, including:

a) Virology
b) TB and Leprosy
c) Bacteriology
are traceable via NPHL’s online system SIMKA (simka.moh.gov.my/result/). The results can be
downloaded and printed by users with authorized logins and passwords.
However, results for Biochemistry, Parasitology and Mycology, Entomology are traceable via phone
and fax at the present time until further notice of results availability via SIMKA. Please see the the
Comprehensive Directory of Infectious Disease, Biochemistry and Entomology Tests for full details.
B. TELEPHONE
All results for samples sent to the Food Division are traceable by phone +60 (3) 6126 1200 (General
Line) or fax. +60 (3) 6140 2249.
The extension numbers of the specific officers to contact are available in the NPHL website:
http://mkak.moh.gov.my (Hubungi Kami > Direktori Staf > Bahagian Makanan)
Page 251
REFERENCES
1. http://mkak.moh.gov.my
2. MKAK Service Handbook 2016
3. Instruction Sheet for WHIRL-PAK Sample Bag, NASCO Canada
4. 3M Product Guide: All-in one Swab for Consistent Surface Sampling, 3M Health Care Food
Safety Department
Page 252
APPENDIX 1
MKAK-BPU-U01/Rev2018
BORANG PERMOHONAN UJIAN MAKMAL (SPESIMEN KLINIKAL)

MAKMAL KESIHATAN AWAM ……………………………
NO RUJUKAN MAKMAL (MKA) :

A. MAKLUMAT PESAKIT
Nama Pesakit: Umur: No Rujukan Pesakit (R/N):
No K.P/ Lain-lain: Jantina: L / P
Warga Negara: Bangsa: Wad:
Alamat pesakit: Pekerjaan: Status perkahwinan Tanda ( √ ) yang berkenaan:
No. Tel.: Bujang Berkahwin Lain-lain
B. TUJUAN PERSAMPELAN Tanda ( √ ) yang berkenaan C. LAIN-LAIN MAKLUMAT

Wabak/ Kluster Pesakit (Ada gejala) Lokaliti kejadian:
Survelan Kes
Diagnostik Kontak
Projek Kluster Sejarah melancong: Ada / Tiada Negara:
Lain-lain Tarikh keluar: Tarikh masuk:
D. RINGKASAN KLINIKAL Tanda ( √ ) yang berkenaan

Tanda dan Gejala Ada (√) Tarikh onset Tanda dan Gejala Ada (√) Tarikh onset
0 5)
1) Demam ( C)
2) Selsema 6)
3) Cirit-birit 7)
4) Muntah 8)
Status & tarikh imunisasi berkaitan: Ada ___________Tarikh___________ Tiada ______________ Tidak diketahui _____________
E. MAKLUMAT SPESIMEN
Jenis Spesimen Jenis ujian dipohon Tarikh diambil Tarikh dihantar Tanda Tangan Pegawai yang mengambil spesimen
(sila cop)
* Nota: Sila rujuk Service Handbook Makmal Kesihatan Awam Kebangsaan untuk maklumat lanjut tentang spesimen
F. BUTIRAN PEMOHON G. BUTIRAN MAKMAL TRANSIT
Nama Nama
Jawatan Jawatan
Tempat bertugas Tempat bertugas
(sila cop) (sila cop)
No H/P: Email: No tel & samb. Email:
KK/PKD/Hospital: Nama Pusat Transit:
Daerah: Negeri: Daerah: Negeri:
H. MAKMAL (untuk kegunaan MKA):

Unit Pengurusan Spesimen Makmal Catatan
0
Suhu: C Jenis sampel: Terima / Tolak
Sampel dlm transport 0
Sampel: Terima / Tolak Suhu: C
media: Ya / Tidak
Nama Penerima : Nama Penerima:
Tarikh & masa: Tarikh & Masa:
Keputusan ujian disahkan oleh : Tarikh:
Page 253
APPENDIX 2
TBIS 20C
Sistem Maklumat TB,KKM
Kementerian Kesihatan Malaysia

Program Kawalan Penyakit TB
Permohonan ujian TB
A. Pusat Pungutan spesimen (Wad/KK/Hospital) : Tarikh Permohonan:
B. Maklumat Pesakit
Nama : No Pengenalan Diri (IC/Paspot) :
Umur : No Telefon : Jantina: M F
Alamat: Warganegara : Malaysia
□ Bukan Malaysia, Nyatakan
………………………………..
Status RVD : Positif Negatif Diabetik? : Ya Tidak
C. Sebab memohon (Tandakan satu) Adakah pesakit telah menerima rawatan ≥ 1 bulan?
Presumptive TB □ Ya Tidak (New Case)
Follow-up TB case (Month of treatment: ……..months) Sekiranya YA,
Contact of TB case No Pendaftaran TB bagi kes adalah: - -
Contact of DRTB case (RR, MDR, XDR, TDR) Klasifikasi Previously Treated TB adalah :
Suspected MDR-TB After Failure of 1st treatment After Failure of retreatment
□ Surveillance of …………………………………………. After loss to follow-up Relapse Others
D. Jenis Specimen : Kahak ( x1 / x2 / x3 ) Spot Pagi lain-lain (nyatakan) : ……………

Tarikh pengambilan spesimen : - - ………………………………....
E. Ujian Di pohon Microskopik Kultur ID & Kerentanan Ubatan (Drug susceptibility)

PCR MTB Xpert MTB/RIF LPA Interferon Gamma Release Assay (IGRA)
F. Maklumat Pemohon
Tandatangan :
Nama :
Jawatan & Cop Rasmi : No.Telefon :
KEPUTUSAN UJIAN MAKMAL (Di isi oleh pihak makmal yang menjalankan ujian)
(Sila gunakan bahagian belakang mukasurat ini sekiranya ruangan tidak mencukupi)
Diuji oleh: Disahkan oleh
Tandatangan: Tandatangan:
Nama: Nama:
Jawatan & Cop Rasmi: Jawatan & Cop Rasmi:
No.Telefon: No.Telefon:
Page 254
APPENDIX 3
MKAK-BPU-D02(rev_Nov_2015)
MAKMAL KESIHATAN AWAM KEBANGSAAN, KEMENTERIAN KESIHATAN MALAYSIA
Lot 1853, Kg Melayu Sungai Buloh, 47000 Sungai Buloh, Selangor Darul Ehsan
Tel: 03-61565109
Fax: 03-61402249/61569654
LABORATORY REQUEST FORM FOR DENGUE AND FLAVIVIRUS
Lab No. (for lab use) :
REQUESTOR INFORMATION
Name :
Post :
Address :
District : State :
Tel. No. : Fax No. : Email :
Purpose of Sampling
a. Dengue (please tick purpose of sampling as b. Flavivirus (please tick purpose of sampling
below) as below)
Outbreak Outbreak
Surveillance Surveillance
Diagnostic Diagnostic
Specimen Category : case Contact
A. PATIENT’S INFORMATION
Name : Age : Date of birth
Sex : Male Female
IC No.
Reference No. : Nationality : Malaysian Non Malaysian
Address (Please state country of origin)_ _ _
Postcode : Occupation :
District : State : Tel. No. :
B. CLINICAL SUMMARY
Fever : T …….oC Diarrhea Laboratory findings at admission
Retro-orbital pain Bleeding tendencies Hb : TWBC : (PN : %; L: %; M : %; E: %)
Maculopapular rash Hepatomegaly Platelets : /mm3 HCT :
Vomitting Shock Dengue NS1 : Date of test :
Myalgia/arthralgia CNS Complications Method :
Dengue IgG : Date of test :
Date of fever onset : (dd/mm/yyyy) Method :
Dengue IgM : Date of test :
Method :
Clinical/Provisional Diagnosis :
Dengue Fever Dengue Hemorrhagic
Dengue Shock Syndrome Death : (dd/mm/yyyy)
Compensated Shock Other (flavivirus).
C. PATIENT’S LOCATION
Clinic Ward ICU
D. SPECIMEN INFORMATION
Type of specimen : Name of Collector :
Date of Collection: (dd/mm/yyyy) Date specimen Received (for lab use) : (dd/mm/yyyy)
E. RESULTS (for lab use only)
Verified by : Date:
Page 255
APPENDIX 4
MAKMAL KESIHATAN AWAM KEBANGSAAN
BORANG PERMOHONAN UJIAN MAKMAL HFMD
No. Rujukan Makmal: MKAK/ENT/20 / )
A. TUJUAN PERSAMPELAN
Wabak O
Survelan (Klinik Sentinel) O
Kes Teruk (Masuk Wad & Umur < 5 tahun) O

B. MAKLUMAT PESAKIT
Nama Pesakit:
No. Kad Pengenalan / Passport: Umur:
Warganegara: Jantina: L / P
Hospital / Klinik Kesihatan: Wad:
R/N: Bangsa :
Negeri: Daerah :
C. MAKLUMAT KLINIKAL
Gejala Tandakan ( √ ) di ruangan berkenaan Tarikh mula
Demam ≥ 38oC
Ulser di mulut & tekak
Maculopapular rash dan / vesikel pada tapak tangan dan
tapak kaki
Tanda dan gejala URTI
Lain-lain
D. MAKLUMAT SPESIMEN KLINIKAL

Jenis Spesimen Tandakan ( √ ) di ruangan Tarikh diambil Tarikh dihantar Pengambil
berkenaan Sampel
Rectal swab
Mouth ulcer
Vesicle swab
Stool
E. MAKLUMAT PEMOHON F. MAKLUMAT MAKMAL TRANSIT* (sekiranya berkenaan)

Tandatangan & Cop Pegawai: Tandatangan & Cop Pegawai:
No. Telefon: No. Telefon:

G. UNTUK KEGUNAAN MAKMAL
Kaunter Penerimaan Sampel Makmal
Tarikh spesimen diterima: Tarikh spesimen diterima:
oC oC
Suhu: Suhu:
Jenis spesimen: Jenis spesimen:
Status: Sampel Diterima / Sampel Ditolak* Status: Sampel Diterima / Sampel Ditolak*
* Sekiranya spesimen ditolak, sila nyatakan sebab: ……………………………………………………………………………………….…..

CATATAN:
Tandatangan & Cop Pegawai: Tandatangan & Cop Pegawai:
Sebarang kemusykilan sila hubungi:

• Makmal Kesihatan Awam Kebangsaan (MKAK) Sungai Buloh, Selangor (u.p. Makmal Isolasi Virus): 03-6126 1200 / 1325
• Sample swab mesti dimasukkan dlm vtm dan suhu penghantaran utk semua sample adalah 2-8 degree celcius
Page 256
APPENDIX 5
MSLF :01I 2004
No. Rujukan Makmal:
MEASLES - BORANG PERMOHONAN DAN KEPUTUSAN UJIAN MAKMAL

A. MAKLUMAT PESAKIT
Negeri: Daerah:
Hospi tal / Klinik Kesihatan:
Nama Pesakit:
No. KIP: Umur: Jantina: L /P
B. MAKLUMAT IMUNISASI MEASLES
lmunisasi measles:
□ Ada □ Tiada □ Tidak diketahui Tarikh dos terakhir diberi:
C. MAKLUMAT KLINIKAL
Gejala (Simptom) Ada/Tiada (Tandakan √ diruang berkenaan) Tarikh mula
Demam
Ruam (maculopapular rash)
Konjunktivitis
Batuk
"Coryza"
D. SPESIMEN KLINIKAL
□Kedua
Spesimen:
□Pertama
Spesimen (tandakan -,/ diruang berkenaan) Tarikh diambil Tarikh penghantaran
Darah / Serum
Sekresi pernafasan (Respiratorysecretion)
Air kencing (Urine)
E. MAKLUMAT PEMOHON
Nama dan Cop Pegawai: No telefon:

No. fax:
e-mail:
Tandatangan:
F. MAKMAL (Untuk Kegunaan Makmal)
Keadaan spesimen: Tarikh terima spesimen:
Spesimen Jenis ujian Keputusan ujian Komen
Darah / Serum
Sekresi pernafasan (Respiratory secretion )
Air kencing (Urine)
Nama dan tandatangan Pegawai Makmal:
Jawatan Pegawai Makmal dan Cop Makmal: Tarikh:
• Nota: Jika spesimen ini adalah spesimen kedua, maklumat mengenai lmunisasi Measles dan Klinikal tidak perlu diisi jika telah diisi pada borang
spesimen pertama.
Spesimen klinikal (darah I sekresi pernafasan I air kencing) hendaklah diambil jika pesakit disyakki sebagai kes measles.
Defini kes (case definition) adalah seperti dinyatakan di belakang
Measles Elimination In Malaysia - Measles Surveillance Manual (1st edition)
Page 257
APPENDIX 6
No. Makmal (untuk kegunaan makmal):
MAKLUMAT PEMOHON (cop rasmi)
Nama : MAKMAL KESIHATAN AWAM KEBANGSAAN
Jawatan : KEMENTERIAN KESIHATAN MALAYSIA
Alamat : Lot 1853, Kg. Melayu Sungai Buloh,
Daerah : Negeri : 47000 Sungai Buloh, Selangor Darul Ehsan Tel :
No. Tel : No. Faks : 03 - 61565109
E-mel : Faks : 03 - 64102249 / 61569654
BORANG PERMOHONAN PENYIASATAN/PEMANTAUAN SAMPEL PERSEKITARAN
A. MAKLUMAT SAMPEL
Jenis Sampel : Tujuan persampelan :

Tarikh Persampelan : Wabak / kluster
Lokasi Persampelan : Survel na
Nama Pegawai Persampelan : Program / projek
No. Kad Kuasa Pegawai Persampelan : Lain-lain :
Jenis ujian :
Analisa Parameter Fizikal

ID Sampel Masa Persampelan Suhu (⁰C) pH Clarity Catatan
B. MAKLUMAT SAMPEL BERKAITAN PENGESANAN LEGIONELLA SAHAJA
Jumlah Tangki/Menara Penyejuk : Tarikh Akhir Penyelenggaraan :

Kaedah yang digunakan untuk penyelenggaraan (termasuk jenis bahan kimia) :
C. MAKLUMAT KES (sekiranya ada) :
Nama kes : Status kes / Hidup

kontak* : Mati
No. K/P atau ID :
Pekerjaan / Pendedahan (Exposure) : * potong mana yang tidak berkenaan
Page 258
D. MAKLUMAT LOKASI PERSAMPELAN
Keadaan Sekitar Lokasi Persampelan :

Premis makanan *(Kekal / Bergerak)
Penternakan haiwan. Nyatakan :
Kawasan Kediaman / Perumahan. Nyatakan :
Aktiviti rekreasi. Nyatakan :
Aktiviti pertanian
Sistem pengurusan sisa *(Baik / Tidak)
Sistem saliran air *(Baik / Tidak)
Kawasan banjir
Kawasan redup / celah batu
Lain-lain :
* potong mana yang tidak berkenaan
Adakah sampel air menjadi sumber bekalan air kepada awam? Ya Tidak
Jenis sumber air : Terawat Tidak Terawat. Nyatakan :
E. LAKARAN LOKASI PERSAMPELAN
Petunjuk :
MKAK/BP/ENV/01 Rev 1 Page 259

APPENDIX 7
Nama PKD:
BORANG PERMOHONAN UJIAN PARAS CHOLINESTERASE

(A) BUTIR PERIBADI
Nama : Umur :
No Kad Pengenalan : Pekerjaan :
Jantina : L /P Bangsa : M/C/I/L Taraf Perkahwinan: Bujang/Berkahwin
Alamat Majikan :
(B) BUTIR KLINIKAL
Berat badan : kg Tinggi : m Tekanan darah : /mmHg
Ada menggunakan racun perosak selain daripada semasa bekerja? Ya
(contoh berkebun atau ladang) Sila tandakan (/) pada kotak. Tidak
Ada mengambil ubat?
(Jika ada sila nyatakan nama ubat-ubat tersebut)
Ada tanda-tanda klinikal berikut (Sila tanda [/] pada kotak) Sejarah klinikal (termasuk
Jaundice Anxiety Staggering gait pengambilan alkohol,
Lymphadenopathy Tremors Mental confusion merokok)
Hepatomegaly Salivation Miosis
Spleenomegaly Lacrimation Hypotension
Lain-lain: Sila nyatakan ..................................
(C) BUTIR PENYEMBURAN RACUN PEROSAK

Jenis racun perosak. (Sila tandakan [/] pada kotak) Organophosphate Carbamate
Topeng muka (mask)
Ada memakai perlindungan diri seperti berikut Sarong tangan
semasa mengendalikan racun perosak? Apron/kot/Baju khas
[Sila tandakan [/] pada kotak.] Kasut but getah
Earplug/earmuff
Goggles
Lain-lain. Sila nyatakan…........................................
Tarikh akhir penyemburan/pendedahan pada
racun perosak (Diisi untuk permohonan baseline shj)
Tempoh masa anggota direhatkan > 30 hari
(Diisi untuk permohonan baseline shj) > 14 hari
(D) UJIAN CHOLINESTERASE (Sila tandakan [/] pada yang berkaitan)
a) Serum BASELINE (Sebelum Penyemburan)
1st Baseline Sample
2nd Baseline Sample Tarikh Pengambilan Masa Pengambilan
rd
3 Baseline Sample
(Jika perlu)
b) Serum POST EXPOSURE (Selepas Penyemburan)
Tarikh Penyemburan
Tarikh Pengambilan darah Masa Pengambilan
Nama Pegawai Perubatan :
Cop dan Pengesahan : Tarikh penghantaran :
Page 260
APPENDIX 8
Page 261
APPENDIX 9
PERMOHONAN UJIAN KERINTANGAN VEKTOR TERHADAP RACUN SERANGGA

Borang Penghantaran Spesimen (KKM/BPS/001/2014)
Negeri:
Daerah :
Lokaliti :
Koordinat GPS :
Minggu epid :
Sejarah penggunaan racun serangga di lokaliti:
Tujuan ( sila tanda √ pilih satu sahaja)

Survelan Wabak
Jenis Ujian (sila tanda √ pilih satu sahaja dan potong yang tidak berkenaan)
Ujian Kerintangan Nyamuk
Pengesanan Parasit Filariasis / Malaria Dalam Nyamuk
Pengesanan Virus Denggi Dalam Nyamuk
Pengesanan Virus JE /Chikungunya Dalam Nyamuk
Page 262
Borang Penghantaran Spesimen (KKM/BPS/001/2014)
Bilangan Spesimen Tarikh
Spesis
No Nama Lokaliti No Sampel Pungutan /
Nyamuk Telur Larva Dewasa Penghantaran
Tangkapan
Nama Pemohon:
Jawatan :
Alamat Tempat Bertugas:
Telefon / Fax Pejabat :
Email :
Page 263
APPENDIX 10
Page 264
LIST OF CONTRIBUTORS
➢ IN ALPHABETICAL ORDER
GROUP I
MICROBIOLOGY
1. AMRISH SHAH B OSMAN 23. NOR FARAH DINA BT PADDY
2. AZIYATI BT OMAR 24. PPKP NURAQILAH BT ABDUL MAJID
3. AZLI BIN ISMAIL 25. NUR HASMIMI BT HASSAN
4. DAWARAH @ THEVARAJ A/L 26. NURIZZAT B MUHAMAD
PALANIAPPAN 27. NURUL AINA MURNI BT CHE AZID
5. PPKP DEENESH KUMAR A/L KRISHNAN 28. WAN NORAINI WAN YUSSOF
6. DR DONAL HUDA BT NASRIL 29. PARVATHY A/P S. SUNDARA RAJOO
7. DR. FARIDAH BT AMIN 30. PUVANESWARY A/P RAMASAMY
8. HADAUAN BIN JENUN 31. REHAN SHUHADA BT ABU BAKAR
9. HAMZAN B MADAN 32. ROHAYATI BT MOHD NAPIAH
10. DR. JOSHITA HEPHZIBAH A/P 33. ROZITAH BINTI OMAR
JOTHIMANICKAM 34. ROZLIANA HANIM BT MAT HASAN
11. KHAIRUL AZAN B HASHIM 35. R. PUSPARANI A/P RAMASAMY
12. KIROSHIKA PILLAI A/P VELL PILAY 36. PPKP SAIFUL AFFIEZAL SAIDINA ALI
13. KUMUTHAMALAR A/P SANGARAN 37. SALFRYNA BINTI ALZAHARI
14. MAHFUZAH BT MOHAMAD AZRANYI 38. SELVANESAN A/L SENGOL
15. MOHD ASRI BIN YAMIN 39. SITI SUBKHIAH BT SATARI
16. MOHD HATIMI B TUKIMAN 40. USHANANTHINY A/P RAVI
17. MOHD SUKRI B IBRAHIM 41. WONG LAI MENG
18. PPKP MUZALIFAH BT MOHD IBRAHIM 42. WAN NUR AFIZA BT WAN MOHD
19. NOOR KASMAWATI BT OTHMAN ARIFIN
20. NORAZIMAH BT TAJUDIN 43. YU KIE A/P CHEM
21. NORIZAH BT ISMAIL 44. ZIRWATUL ADILAH BT AZIZ
22. DR. NOR ZAHRIN BT HASRAN 45. DR. ZULHAINAN B HAMZAH
GROUP II
FOOD
1. GHANI ABU SAMAH 9. SITI KHAIRANI ABD KEDER
2. WAN AINIZA WAN MUSTAPHA 10. TAN CHENG KENG
3. SHARMILI KUPPAN 11. ROSE HELDAWATI CHE ROSE
4. NORHIDAYU IBRAHIM 12. NUR AIN ZAKARIA
5. PRISCILLA DINANG ANAK KASIM 13. ZULIANA ZAKARIA
6. NORHASHIMAH HASHINI 14. MAZLIZA RAMLI
7. NORYANTI RAHMAT 15. PPKP MOHAMMED RAZIMAN B
8. NORULHAIDA ABD. RAZAK RAJAB
GROUP III
BIOCHEMISTRY
1. FARAH SYAHIBAH BT MOHD HARIRI 4. NIK FARHANA BT SAMSUDIN
2. JANAGI NAIDU 5. NIK SYAMIMI BT NIK YUSOFF
3. MASLINDA MANSOR 6. NOOR HAMISSON BT ABAS
GROUP IV
ENTOMOLOGY
1. NURUL HUDA BINTI NORDIN 2. SHARIFAH MILKAH BT SYED ABD
RAHMAN
GROUP V
SAMPLE COLLECTION
1. DR DONAL HUDA BINTI NASRIL 6. NORULHAIDA BT. ABD. RAZAK
2. HANNAH PHOON YIK PHING 7. ROHAYATI BT MOHD NAPIAH
3. MASLINDA BINTI MANSOR 8. SELVANESAN SENGOL
4. MOHD ASRI BIN YAMIN 9. SITI SUBKHIAH BT SATARI
5. NOOR KASMAWATI BINTI OTHMAN 10. USHANANTHINY RAVI
Page 265
ACKNOWLEDGEMENT
We acknowledge the contributions of:

Dato' Dr. Hj. Azman Bin Hj. Abu Bakar, Deputy Director General of Health (Public Health)
Dato' Dr Chong Chee Kheong, Director of Disease Control Division
Dr Zainudin Abdul Wahab, Director of JKWPKLP
Dato’ Dr Khalid Ibrahim, Director of JKNS
The Disease Control Division, MOH
Petaling District Health Office
Lembah Pantai District Health office
Titiwangsa District Health Office
Klang District Health Office
Gombak District Health Office
For all who have contributed to the success of producing this NPHL Test Handbook, directly
or indirectly, we extend our gratitude and appreciation to you.
Page 266
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National Public Health Laboratory Test Handbook 2018 FINAL (28082018)

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THE NATIONAL PUBLIC

NPHL, Ministry of Health Malaysia

Perpustakaan Negara Malaysia Cataloguing-in-Publication Data

THE NATIONAL PUBLIC HEALTH LABORATORY TEST HANDBOOK 2018/

DATO' DR. HJ. AZMAN BIN HJ. ABU BAKAR

DATO' DR CHONG CHEE KHEONG

DR. HANI BINTI MAT HUSSIN

DR HAJAH NOORLIZA BT MOHAMAD NOORDIN

EDITORIAL BOARD MEMBERS

1. DR. NOORLIZA BINTI MOHAMAD NOORDIN---------------------------Editor in Chief

GROUP I GROUP II GROUP III GROUP IV GROUP V

GROUP LEADER GROUP LEADER GROUP LEADER GROUP LEADER

The National Public Health Laboratory (NPHL) is comprised of five

1. To align the content of the test handbook with the core

Infections according to Possible pathogens (in alphabetical Page number

GASTROINTESTINAL TRACT Aeromonas sp. (caviee, hydrophilia, veronii) 10, 32

Quick Guide of Possible Pathogens by Routes of Transmission Page 6

CENTRAL NERVOUS Human herpesvirus 1 (HSV Type 1) 45, 46

BLOOD BORNE INFECTIONS Brugia malayi 38

ZOONOTIC INFECTIONS Leptospira interrogans 57

SEXUALLY TRANSMITTED Neisseria gonorrhoeae 90

CUTANEOUS INFECTIONS Mycobacterium leprae 88

EMERGING AND SPECIAL Orthopoxvirus 98

Quick Guide of Possible Pathogens by Routes of Transmission Page 7

IMPORTANT POINTS TO NOTE:

Tel No. 03-

Outbreak IFAT/ Sputum 1-3 ml Sterile 2-8°C √ 1. Sample should

Electron Microscopy Unit 1320 / 1335

A. The Comprehensive Directory of Infectious Disease Tests in NPHL Page 9

Tel No. 03-

Lung 1.5cm Sterile

Electron Microscopy Unit 1320 / 1335

collection at 2°C – 8°C

A. The Comprehensive Directory of Infectious Disease Tests in NPHL Page 10

Tel No. 03-

Fresh NA Cary Blair 5 days Daily 1329/ √ √ √ √ √ 1. Please refer to

unavoidable, send at 2°C – 8°C.

Rectal NA Cary Blair 5 days Daily 1329/ √ √ √ √ √ 1. Please refer to

send at 2°C – 8°C

2. Ensure that the

A. The Comprehensive Directory of Infectious Disease Tests in NPHL Page 11

Tel No. 03-

Astrovirus Outbreak EM/ Fresh 5 ml / Sterile 2-8°C √ 1. Sample should

Molecular Unit 1321

Send within 2 hours after collection at 2°C – 8°C

A. The Comprehensive Directory of Infectious Disease Tests in NPHL Page 12

Tel No. 03-

Fresh NA Cary Blair 5 days Daily 1329/ √ √ √ √ √ 1. Please refer to

unavoidable, send at 2°C – 8°C.

Rectal NA Cary Blair 5 days Daily 1329/ √ √ √ √ √ 1. Please refer to

2. Ensure the swab

A. The Comprehensive Directory of Infectious Disease Tests in NPHL Page 13

Tel No. 03-

Toxin Bacterial NA slant 7 days Daily 1329/ √ Inoculate pure

Send at 2°C – 8°C

Outbreak or Real- 1-3 Sterile 48 Daily 1329/ √ √ √ √ √ 1. Please refer to

Nasopharyngeal Aspirates (in-patient)

Send immediately after collection at 2°C – 8°C

A. The Comprehensive Directory of Infectious Disease Tests in NPHL Page 14

Tel No. 03-

NA Amies clear 48 Daily 1329/ √ √ √ √ √ 1. Please refer to

Nasopharyngeal swab (contact)

1-3 NA 10 days Daily 1329/ √ √ √ √ 1. Please refer to

Nasopharyngeal Aspirates (in-patient)