You are on page 1of 21

Int. J. Agri. Agri. R.

International Journal of Agronomy and Agricultural Research (IJAAR)


ISSN: 2223-7054 (Print) 2225-3610 (Online)
http://www.innspub.net
Vol. 8, No. 5, p. 39-59, 2016
RESEARCH PAPER OPEN ACCESS

Productivity of some forage grasses under foliar sprinkler


irrigation and foliar application of potassium nitrate under
salinity stress

A.A. Kandil, A.E. Shareif *

Department of Agronomy, Mansoura University, Egypt


Article published on May 12, 2016
Key words: Alfalfa, Rhodes and Blue panic, Potassium nitrate, water use efficiency.

Abstract
Field experiment was conducted during 2013/2014 and 2015 seasons was aimed to evaluate productivity of
Rhodes and Blue panic grasses as well as Alfalfa under sprinkler irrigation with foliar application of potassium
nitrate that enable plants to cope with water stress. Each two forage grasses beside alfalfa were conducted in
separate experiments. Irrigation every 10 days surpassed in total chlorophyll, leaf area, plant height, number of
stems/m2, forage green yield/fed and forage dry matter yield/fed than those irrigated every 20 days. Highest
percentages of water use efficiency, crude fiber and nitrogen free extract as well as yield/fed of crude protein,
crude fiber, ether extract, ash and nitrogen free extract were produced from irrigation every 7 days. However,
highest percentages of crude protein, ether extract and ash were produced from irrigation every 14 days. Forage
species significantly differed on total chlorophyll, leaf area/plant, plant height, average of number of stem/cm 2,
forage green yield/fed and forage dry matter yield/fed. A significant effect due to forage species on percentages of
water use efficiency, crude protein and fiber, extracting ether, ash and nitrogen free extract and nitrogen free
extract as well as crude fiber, extracting ether, ash and nitrogen free extract yield/fed. Sown Rhodes grass
produced maximum forage green and forage dry matter yield/fed and its quality compared with other studied
forage crops. Foliar spraying of potassium nitrate at 15 ppm significantly increased total chlorophyll, leaf area,
plant height, number of stems/m2, forage green and forage dry matter yield/fed. The results showed that highest
percentages of water use efficiency, crude protein, crude fiber and ether extract, and nitrogen free extract and
nitrogen free extract as well as crude fiber, extracting ether, ash and nitrogen free extract yield/fed. It could be
stated that irrigation every 10 days and sown Rhodes grass and foliar spraying of potassium nitrate at 15 ppm
maximized forage green and forage dry matter yield/fed.
* Corresponding Author: Shareif AE  shariefali42@gmail.com

Kandil and Shareif


Page 39
Int. J. Agri. Agri. R.

Introduction Ca(NO3)2 at 0.406%. Al-Soqeer and Al-Ghumaiz


Alfalfa is the main forage crop in newly reclaimed soil (2012) showed that there was a stronger response in
in Egypt. In arid land, especially in Egypt, irrigation grasses species dry matter yield to irrigation
water is very limited and recently, the salinity soils treatment. Rhodes grass had superior performance
and waste water were increased. Water is the single for all agronomic character comparing with other
most limiting resource for world agriculture and food genotypes. Moosavi et al. (2012) showed that the 5
production, highly exceeding other key limitations. days irrigation interval allocated to itself the highest
Efficient water use by irrigation systems is becoming vegetative growth, yield and more quality of forage
increasingly important, especially, in arid and semi- but with respect to water limitation and intense need
arid regions with limited water sources. In to forage in the region, in order to scrounge in the
agricultural practice, the sufficient and balanced amount of consumption water in the level unit and
application of irrigation water and nutrients are increase of under cultivation lands, we can use 10
important methodology to obtain maximum yield per days irrigation interval for the planting of forage
unit area. Several investigators studied the effect of sorghum. Abusuwar and Eldin (2013) pointed out
irrigation water amounts or levels on some important that the two grasses (Teff and Rhodes grass) were
plant characters. Large amount of water is used in more drought tolerant than two legumes (alfalfa and
field production of food crops, leading to a deficit of Siratro) as they resulted in higher plant density,
fresh water resources in many arid or semi-arid areas higher number of leaves and higher productivity
in the world (Zhu, 2002). Al-Suhaibani (2006) under all treatments. Zeinab Mobarak et al. (2013)
reported that both 1st and 2nd cut yield was about 85% reported that foliar application of magnesium nitrate
of the total obtained forage. Concerning effect for as foliar fertilization has positively affected growth
growing season and irrigation intervals on the forage and development of maize plants through increasing
yield, expanding irrigation interval from 3 to 7 and 11 concentration and uptake of macro and
days decreased the potential yield from 143.6 to 123 micronutrients by the plant tissues. Moreover, Osman
and 85.3 t/ha, respectively. Al-Soqeer and Al- et al. (2014) concluded that Rhodes grass significantly
Ghumaiz (2012) showed that a significant effect for out yielded forage in all cuts other than the first one.
cuts on forage dry matter yield. recent years Ali et al. (2007) stated that temporal prediction of soil
freshwater shortages have led to restrictions on the moisture and evapotranspiration (ET) plays a crucial
use of drinking water for landscape irrigation and an role in irrigation water management. Mirdad et al.
increase in the use of low quality water sources such (2009) indicated that the main effects of each
as waste-water and brackish water. They added that irrigation dates (every 2, 4, 6 and 8 days) significantly
expanding irrigation interval decreased all agronomic differed vegetative growth and yield characters.
characters. There was a stronger response in grasses Akram and Ashraf (2009) concluded that exogenous
species dry matter yield to irrigation treatment. application of potassium nitrate increased the
(Thomas et al., 2006). In addition, Abusuwar and photosynthetic rate and leaf turgor which in turn
Eldin (2013) pointed out that irrigation every other resulted enhanced growth and yield in sunflower
day resulted in a significantly higher plant density, plants subjected to salt stress. Foliar application of
higher number of leaves per plant and higher fresh KNO3 was found to increase nitrate content of
and dry yields compared to the longer irrigation ryegrass leaves under non-saline or saline condition
interval (irrigation every 6 days). Sarkar and Malik (Tabatabaei and Fakhrzad, 2008). Jabeen and Ahmed
(2001) stated that foliar spray of KNO3 at 0.50% (2011) found that application of KNO3 significantly
during 50% flowering stage showed maximum values reduced the increasing tendency of Na+ and Cl- and
of pods/plant, length of pod, seeds/pod and 1000 increased leaf area, its fresh and dry weight per plant,
seed weight; it was significantly superior to water NO3- and soluble protein concentration and NR
spray and unsprayed control, but was on par with activity in leaves irrespective to the growth of plant

Kandil and Shareif


Page 40
Int. J. Agri. Agri. R.

under non saline or saline conditions. Furthermore,


conditions. Whereas, Gimeno et al. (2014) concluded Number of stems per/m2: Number of stems per plant
that a 2% foliar KNO3 application can enhance the counted per sample i.e. 0.25 m2 and then transferred
tolerance of citrus plants to water stress by increasing to number of stems/m2.
the osmotic adjustment process. Therefore, the
objective of this investigation was aimed to introduce Plant height (cm): Ten stems was taken at random
forage grasses tolerant to water stress in new from each plot. Stem length was measured from base
reclaimed soils to overcome the shortage in summer of stem till its end.
forage production in Egypt.
Leaf area/plant in cm2 was measured using Field
Materials and methods Portable Leaf Area Meter AM-300 (Bio-Scientific,
Experimental treatments Ltd., Great Am well, Herefordshire, England).
Three separate experiments carried out for the two
forage grasses beside alfalfa. This experiment aimed Forage green yield per faddan: Two random samples
to evaluate productivity of studied forage grasses taken using a square wooden frame 100×100 cm from
under normal of sprinkler irrigation with foliar each plot. Samples cut by sickle at about 3 cm height
application of potassium nitrate which play adaptive from soil surface and then weighted. Mean weight per
roles in plant stress tolerance. Each experiment square meter calculated then transformed as forage
included two treatments of irrigation. yield/feddan.

1. Irrigation every 10 days or 20 days. Plastic sheet Forage dry matter yield/feddan: Two random
put in soil horizontally at depth 100 cm between samples of known weight (100 gm) taken from each
irrigation treatments to prevent irrigation water plot. These samples dried at 70 0 C for 24 hr and then
movement. at 105 0 C till reached a constant weight. The dry
matter yield/ feddan calculated using the following
2. Foliar application of potassium nitrate affects the equation:
tolerance of salinity of some forage i.e. foliar spraying Dry matter yield/feddan = (Green yield per feddan) ×
of potassium nitrate i.e. 0, 5, 10 and 15 mg/L were (known weight of dry sample) ÷ (known weight of
applied as foliar spray after three weeks of each cut. green sample).
The experiment was laid out in split plot design with
four replications. The three grasses Rhodes grass Water use efficiency (WUE): Water use efficiency
(Chloris gayana, Kunth), Blue grass (Panicum (kg/m3) according to Wright, 1988. The consumed
antidotale, Retz.), and Alfalfa (Medicago sativa, L.) water (m3/ha) was estimated as follows:
assigned in three separate experiments. The two
sprinkler water irrigation every 10 days or 20 days Consumed Water (m3/ha) = (Moisture percentage in
occupied main plots. The sub plot treatment was field capacity- soil moisture percentage) × root depth
allocated with four rates of foliar spraying of × Soil bulk density (1.76) × 10000
potassium nitrate i.e. 0, 5, 10 and 15 mg/L.
Total yield (kg / ha)
WUE =

Studied Character Consumed water (m 3 / ha)


The following data were collected in each cut in the
Where: WUE is Water use efficiency.
three experiments
Total chlorophyll (SPAD): Total chlorophyll (SPAD):
Chemical analysis
Chlorophyll content in leaves samples was assessed
Crude protein (CP): The wet ash will have prepared
by SPAD-502 (Minolta Co. Ltd., Osaka, Japan).
and nitrogen be determined calorimetrically in the

Kandil and Shareif


Page 41
Int. J. Agri. Agri. R.

acid digest using the method recommended by Koch per faddan was calculated by multiplying nitrogen
and McMeekin (1924). Then crude protein percent free extract × yield of dry matter in Kg/faddan.
was calculated by multiplying total nitrogen percent ×
6.25 as described by Bolton and McCarthy (1962). Statistical analysis:
Moreover, crude protein in Kg/faddan was calculated A combined analysis between the three studied forage
by multiplying crude protein percent × dry matter crops was done to obtain the main effect of the three
yield in Kg/faddan. forage crops. In 2013/2014 season green fodder three
cuts and dry matter analysis was carried out. Data
Ether extract (EE): Soxhelt apparatus will be used for was subjected to statistical analysis and means
determination of ether extract percent, heating by compare using LSD test at 5% level according to
electric heater; cold water at 80o C was used through Gomez and Gomez (1991). The data were analyzed
the condenser Ethyl ether that preferred for statistically following RCBD design by MSTAT-C
extraction which continued for not less than 8 hrs. computer package developed by Russel (1986).
(rate of siphoning is six per hr.) These methods are
recommended by official and tentative methods of Results and discussion
A.O.A.C. (2000). Moreover, ether extract in Cutting effects
Kg/faddan was calculated by multiplying ether extract The results presented in Table (1) showed that means
percent X yield of dry matter in Kg/faddan. of total chlorophyll (SPAD), leaf area (cm2), plant
height (cm), number of stems/m2, forage green
Crude fiber (CF): The usual weaned method was used yield/fed and forage dry matter yield/fed as affected
for determination of fiber percent; boiling for 30 min by cuttings, irrigation intervals, forage species and
took place in a suitable beaker under reflux. Filter proline concentrations foliar application. The results
medium is a suitable filter paper (corrugated and clearly showed that cuttings insignificantly affected
both acid and alkali resistant) be fitted into suitable total chlorophyll (SPAD), leaf area (cm2), plant height
Buckner flask attached to vacuum pump. Final (cm), number of stems/m2, forage green yield/fed and
washing after NaOH treatment was hot water, 5% forage dry matter yield/fed. Concerning to chemical
HCl, hot water, alcohol and ether. Suction will be dry forage composition, the results presented in
continued till almost dry, then the residue was Tables (2 and 3) clearly showed insignificant effect
quantitatively transformed out of the filter paper into due to cuttings on percentages of water use efficiency
a suitable crucible by gentle tapping and using a crude protein and fiber, extracting ether, ash and
suitable brush. Drying at 105o C before ashing in the nitrogen free extract as well as crude fiber, extracting
usually way. Moreover, crude fiber in Kg/faddan was ether, ash and nitrogen free extract yield/fed during
calculated by multiplying crude fiber percent × yield 2013/2014 and 2015 seasons.
of dry matter in Kg/faddan.
Irrigation intervals effect
Ash content: A 5 grams of dry matter was burned in a The results in Table (1) showed that irrigation
muffle furnace at 600o C for four hrs. and then ash intervals significantly affected total chlorophyll
percent will be calculated. Moreover, ash in (SPAD), leaf area (cm2), plant height (cm), number of
Kg/faddan was calculated by multiplying ash content stems/m2, forage green yield/fed and forage dry
× yield of dry matter in Kg/faddan. matter yield/fed. The results indicated that irrigation
every 10 days surpassed in total chlorophyll, leaf area
Nitrogen free extracts (NFE): It was calculated by (cm2), plant height (cm), number of stems/m2, forage
using the following equation: Nitrogen free extract = green yield/fed and forage dry matter yield/fed than
100 – (crude protein percent + crude fiber percent + those irrigated every 20 days. The corresponding
ether extract percent + ash percent). Then, amounts results were 16.09, 20.79, 67.04, 318.6, 3.311 and

Kandil and Shareif


Page 42
Int. J. Agri. Agri. R.

2.052, respectively. The lowest values of total compared to the longer irrigation interval (irrigation
chlorophyll, leaf area (cm2), plant height (cm), every 6 days).
number of stems/m2, forage green yield/fed and
forage dry matter yield/fed were produced from With respect to results presented in Tables (2 and 3)
irrigation every 20 days, which were 13.01, 12.41, results clearly indicated a significant effect due to
33.05, 158.1, 0.998 and 0.628, respectively. Al- irrigation intervals on percentages of water use
Suhaibani (2006) reported that a significant effect for efficiency crude protein and fiber, extracting ether,
growing season and irrigation intervals on the forage ash and nitrogen free extract as well as crude fiber,
yield, expanding irrigation interval from 3 to 7 and 11 extracting ether, ash and nitrogen free extract
days decreased the potential yield from 143.6 to 123 yield/fed during 2013/2014 and 2015 seasons.
and 85.3 t/ha, respectively. Al-Soqeer and Al- Highest percentages of water use efficiency, crude
Ghumaiz (2012) showed that expanding irrigation fiber and nitrogen free extract as well as yield/fed of
interval decreased all agronomic characters. There crude protein, crude fiber, ether extract, ash and
was a stronger response in grasses species dry matter nitrogen free extract were produced from irrigation
yield to irrigation treatment. Abusuwar and Eldin every 7 days. However, highest percentages of crude
(2013) found that irrigation every other day resulted protein, ether extract and ash were produced from
in a significantly higher plant density, higher number irrigation every 14 days.
of leaves per plant and higher fresh and dry yields

Table 1. Means of total chlorophyll (SPAD), leaf area (cm2), plant height (cm), number of stems/m2, forage
green yield/fed and forage dry matter yield/fed as affected by cuttings, irrigation treatments, forage species,
nitrate potassium concentrations and their interactions of three cuts during 2013/2014 and 2015 seasons.
Characters Total leaf
Plant height Number of Forage green Dry matter
Treatments chlorophyll area/plant
(cm) stems/m2 yield t/fed yield t/fed
(SPAD) (cm2)
C- Cutting effects
1st cut 14.57 16.55 50.25 236.7 2.066 1.287
2nd cut 14.40 16.66 50.03 237.7 2.148 1.355
3rd cut 14.27 16.33 49.63 237.9 2.178 1.342
4th cut 41.41 16.72 21.45 5.9.1 2.186 4..21
5th cut 41.41 16.73 21.49 511.4 2.194 4..44
LSD at 5 % N.S. N.S. N.S. N.S. N.S. N.S.
I-Irrigation intervals
Every 10 days 16.09 20.79 67.04 318.6 3.311 2.052
Every 20 days 13.01 12.41 33.05 158.1 0.998 0.628
F- Test at 5 % * * * * * *
F-Test C × I * NS * NS N.S. *
F Forage species
Alfalfa 15.72 13.40 37.94 132.0 1.141 0.756
Rhodes grass 14.80 20.59 65.97 340.6 3.117 1.913
Blue panic grass 13.14 15.80 46.23 242.3 2.207 1.351
LSD at 5 % 0.13 0.05 0.32 0.16 0.031 0.054
F- Test C×F NS NS NS NS * *
I×F * * * * * *
C×I× F NS NS NS NS * N.S.

Kandil and Shareif


Page 43
Int. J. Agri. Agri. R.

Characters Total leaf


Plant height Number of Forage green Dry matter
Treatments chlorophyll area/plant
(cm) stems/m2 yield t/fed yield t/fed
(SPAD) (cm2)
N-Nitrate Potassium
0 ppm 13.91 15.89 49.01 233.6 2.076 1.281
10 ppm 14.44 16.53 50.11 239.2 2.161 1.330
15 ppm 14.89 17.39 51.03 242.2 2.228 1.410
LSD at 5 % 0.11 0.15 0.32 0.16 0.032 0.054
C×N NS NS NS NS * N.S.
I×N * * * * * *
F×N NS NS NS NS N.S. *
C×F×N NS NS NS NS NS NS
C×I×N NS NS NS NS * NS
I×F×N NS NS NS NS NS NS
C × I× F × N NS NS NS NS NS NS
N. S.= Not significant, *= significant at 5%, **= significant at 1%.

Table 2. Percentages of Water Use Efficiency, Crude Protein, Crude Fiber, Ether Extract, Ash and Nitrogen free
extract as affected by cuttings, irrigation treatments, forage species, nitrate potassium concentrations and their
interactions of three cuts during 2013/2014 and 2015 seasons.
Water Use Ether Ash Nitrogen
Crude Crude
Treatments Efficiency Extract content % free extract
Protein % Fiber %
WUE% % %
C- Cutting effects
1st cut 5.51 41.44 24.09 2.05 11.22 47.95
2nd cut 5..5 41.42 24.04 2.08 11.31 48.07
3rd cut 5..2 41.42 24.02 2.06 11.25 48.27
4th cut 5.11 41.1. 24.08 2.06 11.26 48.11
5th cut 5.14 41.2. 24.11 2.10 11.27 48.06
LSD at 5 % N.S. N.S. N.S. N.S. N.S. N.S.
I-Irrigation intervals
Every 10 days 3.59 41.11 23.63 2.03 11.20 48.56
Every 20 days 1.11 41.45 24.51 2.12 11.32 47.63
F-Test at 5 % * * * * * *
F-Test C×I N.S. N.S. N.S. N.S. N.S. N.S.
F Forage species
Alfalfa 1.28 49.45 16.74 2.38 12.82 49.19
Rhodes grass 3.40 44..4 25.55 2.15 10.54 50.22
Blue panic grass 2.36 4..12 29.91 1.69 10.43 44.87
LSD at 5 % 0.05 1.41 0.12 0.05 0.11 0.14
F- Test C × F N.S. N.S. N.S. N.S. N.S. N.S.
F- Test I × F * * * * * *
F- Test C × I× F N.S. N.S. N.S. N.S. N.S. N.S.
N-Nitrate Potassium
0 ppm 2.27 41..1 23.58 1.95 10.79 49.29
10 ppm 2.36 41.41 24.08 2.07 11.24 48.16
15 ppm 2.41 41.99 24.54 2.19 11.76 46.83
LSD at 5 % 0.04 1.45 0.09 0.05 0.07 0.45
C×N NS NS NS NS N.S. N.S.
I×N NS NS NS NS NS NS
F×N * NS * NS * N.S.
C×F×N NS NS NS NS NS NS
C×I×N NS NS NS NS NS NS
I×F×N NS NS NS NS NS NS
C × I× F × N NS NS NS NS NS NS
N. S.= Not significant, *= significant at 5%, **= significant at 1%.

Kandil and Shareif


Page 44
Int. J. Agri. Agri. R.

Table 3. Means of Crude Protein, Crude Fiber, Ether Extract, Ash and Nitrogen free extract yield/fed as affected
by cuttings, irrigation treatments, forage species, nitrate potassium concentrations and their interactions of three
cuts during 2013/2014 and 2015 seasons.
Crude Ether Nitrogen free
Crude Fiber Ash content
Treatments Protein Extract extract yield
Yield Kg/fed yield Kg/fed
Yield Kg/fed Yield Kg/fed Kg/fed
C- Cutting effects
1st cut 415.9 322.5 24.8 140.5 623.6
2nd cut 411.4 328.8 26.7 143.9 625.9
3rd cut 419.4 336.2 26.8 145.5 654.2
4th cut 441.. 341.1 27.1 143.2 659.9
5th cut 445.4 341.7 27.6 148.7 662.6
LSD at 5 % N.S. N.S. N.S. N.S. N.S.
I-Irrigation intervals
Every 10 days 274.0 205.8 40.0 222.2 992.4
Every 20 days 41.2 162.4 13.3 68.6 298.2
F-Test at 5 % * * * * *
F-Test C × I N.S. N.S. N.S. N.S. N.S.
F Forage species
Alfalfa 143.3 124.3 18.1 97.0 773.0
Rhodes grass 216.4 482.2 40.0 200.9 970.7
Blue panic grass 178.0 395.7 21.8 138.3 592.1
LSD at 5 % 4.1 6.5 1.9 2.9 23.0
F- Test C× F N.S. N.S. N.S. N.S. N.S.
I×F * * * * *
C × I× F N.S. N.S. N.S. N.S. N.S.
N-Nitrate Potassium
0 ppm 167.9 316.2 24.4 133.9 636.7
10 ppm 178.6 333.0 26.2 145.0 643.7
15 ppm 494.5 353.1 29.3 157.2 655.4
LSD at 5 % 5.9 4.8 1.7 2.4 8.0
C×N NS NS NS N.S. N.S.
I×N NS NS NS NS NS
F×N * * NS * N.S.
C× F × N NS NS NS NS NS
C×I×N NS NS NS NS NS
I×F×N NS NS NS NS NS
C×I×F×N NS NS NS NS NS
N. S.= Not significant, *= significant at 5%, **= significant at 1%.

Interaction between cuttings and irrigation intervals results indicated that the fifth cut and irrigation every
effect 10 days recorded highest values of total chlorophyll
Regarding the interaction between cuttings and without significant differences, which was 16.52.
irrigation intervals on total chlorophyll (SPAD), the However, the lowest values of total chlorophyll were
results indicated that this interaction significantly obtained from the third cut and irrigation every 20
affected total chlorophyll as shown in Table (4). The days, which was 12.62.
Kandil and Shareif
Page 45
Int. J. Agri. Agri. R.

With respect to the interaction effect between cuttings Concerning to the interaction between cuttings and
and irrigation intervals on leaf area/plant (cm2), the irrigation intervals on fresh forage yield/fed, the
results in Table (1) indicated that this interaction results indicated that this interaction significantly
insignificantly affected leaf area/plant (cm2). affected forage green yield. The results in Table (6)
clearly revealed that highest forage green yield/fed
Table 4. Averages of total chlorophyll (SPAD) as was produced from the fifth cut and irrigation every
affected by the interaction between cuttings and 10 days, which was 3.989 t/fed. However, the lowest
irrigation intervals during 2013/2014 and 2015 forage green yield was obtained from the second cut
seasons. and irrigation every 20 days, which was 0.993 t/fed,
Irrigation intervals respectively.
Cuttings
Every 10 days Every 20 days
1st cut 15.84 13.31 Table 6. Averages of forage green yield (ton/fed) as
2nd cut 15.92 12.89 affected by the interaction between cuttings and
3rd cut 15.92 12.62 irrigation intervals during 2013/2014 and 2015
4th cut 16.27 13.01 seasons.
5th cut 16.52 13.23 Irrigation intervals
Cuttings
LSD at 5 % 0.23 Every 10 days Every 20 days
1st cut 3.129 1.005
Concerning to the interaction between cuttings and 2nd cut 3.302 0.993
irrigation intervals on plant height, the results in 3rd cut 3.362 0.994
Table (5), the results indicated that this interaction 4th cut 3.373 0.999
significantly affected plant height. The results 5th cut 3.989 0.999
revealed that the tallest plants were produced from LSD at 5 % 0.007
the fourth or fifth cut and irrigation every 10 days
without significant differences (67.5 cm). However, With respect to the interaction between cuttings and
the shortest plants were obtained from the third cut irrigation intervals on forage dry matter yield/fed, the
and irrigation every 20 days, which was 32.4 cm. results indicated that this interaction significantly
affected forage dry matter yield/fed as shown in Table
Regarding the interaction effect between cuttings and (7). The results revealed that highest forage dry
irrigation intervals on number of stems/m2, the matter yield/fed was produced from the fifth cut and
results in Table (1) indicated that this interaction irrigation every 10 days, which was 2.086 t/fed.
insignificantly affected number of stems/m2. However, the lowest forage dry matter yield/fed was
obtained from the second or third cut and irrigation
Table 5. Means of plant height (cm) as affected by every 20 days without significant differences, which
the interaction between cuttings and irrigation was 0.620 t/fed.
intervals during 2013/2014 and 2015 seasons.
Irrigation intervals Regarding the interaction effect between cuttings and
Cuttings
Every 10 days Every 20 days irrigation intervals on percentages of water use
1st cut 66.6 33.9 efficiency, crude protein and fiber, extracting ether,
2nd cut 66.8 33.2 ash and nitrogen free extract and nitrogen free extract
3rd cut 66.8 32.4 as well as crude fiber, extracting ether, ash and
4th cut 67.5 32.8 nitrogen free extract yield/fed during 2013/2014 and
5th cut 67.5 32.9 2015 seasons, the results in Tables (2 and 3) indicated
LSD at 5 % 0.6 that this interaction insignificantly affected these
traits during 2013/2014 and 2015 seasons.

Kandil and Shareif


Page 46
Int. J. Agri. Agri. R.

Table 7. Averages of forage dry matter yield amount of consumption water in the level unit and
(ton/fed) as affected by the interaction between increase of under cultivation lands, we can use 10
cuttings and irrigation intervals during 2013/2014 days irrigation interval for the planting of forage
and 2015 seasons. sorghum. Abusuwar and Eldin (2013) pointed out
Irrigation intervals that the two grasses (Teff and Rhodes grass) were
Cuttings
Every 10 days Every 20 days more drought tolerant than two legumes (alfalfa and
1st cut 1.951 0.622 Siratro) as they resulted in higher plant density,
2nd cut 2.081 0.628 higher number of leaves and higher productivity
3rd cut 2.063 0.620 under all treatments. Zeinab Mobarak et al. (2013)
4th cut 5.114 1.4.1 reported that foliar application of magnesium nitrate
5th cut 5.144 1.4.9 as foliar fertilization has positively affected growth
LSD at 5 % 0.010 and development of maize plants through increasing
concentration and uptake of macro and

Forage species effect micronutrients by the plant tissues. Recently, Osman

The results in Table (1) clearly indicated that forage et al. (2014) concluded that Rhodes grass significantly

species significantly differed on total chlorophyll, leaf out yielded forage in all cuts other than the first one.

area/plant (cm2), plant height (cm), average of


number of stem/cm2, forage green yield/fed and Regarding to results presented in Tables (2 and 3)

forage dry matter yield/fed. Highest values of total results clearly indicated a significant effect due to

chlorophyll was produced from sown alfalfa (15.72). forage species on percentages of water use efficiency,

However, the lowest values of total chlorophyll were crude protein and fiber, extracting ether, ash and

produced from sown Blue panic grass (13.14). The nitrogen free extract and nitrogen free extract as well

results showed that Rhodes grass recorded highest as crude fiber, extracting ether, ash and nitrogen free

values of leaf area/plant (cm2), plant height (cm), extract yield/fed during 2013/2014 and 2015 seasons.

average of number of stem/cm2, forage green Highest percentages of water use efficiency, and

yield/fed and forage dry matter yield/fed. The nitrogen free extract as well as crude fiber, extracting

corresponding data were 20.59, 65.97, 340.6, 3.117 ether, ash and nitrogen free extract yield/fed sown

and 1.913, respectively. However, the lowest values Rhodes grass during 2013/2014 and 2015 seasons

were obtained from sown alfalfa, which were 13.40, were obtained from. The corresponding data were

37.94, 132.0, 1.141 and 0.756, respectively. Sarkar and 3.40, 50.22% and 216.4, 482.2, 40.0, 200.9 and 970.7

Malik (2001) stated that foliar spray of KNO3 at Kg/fed, respectively. Highest percentages of crude

0.50% during 50% flowering stage showed maximum protein, extracting ether and ash percentages from

values of pods/plant, length of pod, seeds/pod and sown Alfalfa during 2013/2014 and 2015 seasons. The

1000 seed weight; it was significantly superior to corresponding data were 19.12, 2.38 and 12.82%,

water spray and unsprayed control, but was on par respectively. Efficient use of water resources can be

with Ca (NO3)2 at 0.406%. Al-Soqeer and Al-Ghumaiz made possible through the assessment of crop water

(2012) showed that there was a stronger response in requirements and proper scheduling of irrigation.

grasses species dry matter yield to irrigation Hashim et al. (2012) determined the water

treatment. Rhodes grass had superior performance requirements and crop water productivity of different

for all agronomic character comparing with other seasonal and forage crops cultivated in the Makkah

genotypes. Moosavi et al. (2012) showed that the 5 region of Saudi Arabia and concluded that in forage

days irrigation interval allocated to itself the highest crops, the water requirements varied from 962.75 mm

vegetative growth, yield and more quality of forage in Sudan grass to 1922.50 mm in alfalfa. The total

but with respect to water limitation and intense need crop water consumption (mm) was highest in alfalfa

to forage in the region, in order to scrounge in the (1922.50) followed by Rhodes grass (1821.94), blue

Kandil and Shareif


Page 47
Int. J. Agri. Agri. R.

panic grass (1287.76) and Sudan grass (962.75). Blue interaction significantly affected forage dry matter
panic grass can be recommended for cultivation as a yield/fed. The results revealed that highest forage dry
forage crop. Kamel et al. (2012) demonstrated that matter yield/fed was obtained from the fifth cut and
optimal irrigation scheduling requires accurate sown Rhodes grass, which were 1.92. t/fed. On
estimates of crop evapotranspiration. Whereas, Sir contrary, the lowest forage dry matter yield/fed was
and Ahmed (2014) reported that the shorter irrigation recorded from the first cut and sowing alfalfa, which
intervals (7 and 14 day) were statistically significant was 0.718 t/fed.
over the longer interval (21days).
With respect to the interaction effect between cuttings
Interaction between cuttings and forage species and irrigation intervals on percentages of water use
effect efficiency, crude protein and fiber, extracting ether,
With respect to the interaction effect between cuttings ash and nitrogen free extract and nitrogen free extract
and forage species on total chlorophyll, leaf as well as crude fiber, extracting ether, ash and
area/plant (cm2), plant height (cm) and average nitrogen free extract yield/fed during 2013/2014 and
number of stem/cm2, the results in Table (1) showed 2015 seasons, the results in Tables (2 and 3) indicated
insignificant effected on total chlorophyll, leaf that this interaction insignificantly affected these
area/plant (cm2), plant height (cm) and average traits during 2013/2014 and 2015 seasons.
number of stem/cm2.
Table 9. Means of forage dry matter yield (ton/fed)
Regarding to the interaction effect between cuttings as affected by the interaction among cuttings and
and forage species on forage green yield/fed, the forage plant species during 2013/2014 and 2015
results in Table (8) clearly indicated this interaction seasons.
significantly affected forage green yield/fed. The Forage species
Cuttings
results showed that highest forage green yield/fed Alfalfa Rhodes grass Blue panic grass
was produced from the fifth cut and sowing Rhodes 1st cut 0.718 1.863 1.281
grass (3.160 t/fed). On contrary, the lowest forage 2nd cut 0.758 1.888 1.418
green yield/fed was recorded from the first cut of 3rd cut 0.761 1.921 1.343
alfalfa (1.066 t/fed). 4th cut 1.149 4.911 4..2.
5th cut 1.111 4.92. 4..45
Table 8. Means of forage green yield ton/fed as LSD at 5 % 0.012
affected by the interaction between cuttings and
forage species during 2013/2014 and 2015 seasons. Interaction between irrigation intervals and forage
Forage species species effect
Cuttings Rhodes Blue panic Regarding to the interaction between irrigation
Alfalfa
grass grass treatments and forage plant species on total
1st cut 1.066 3.020 2.115 chlorophyll (SPAD), the results in Table (10) clearly
2nd cut 1.149 3.108 2.187 showed that this interaction significantly affected
3rd cut 1.157 3.142 2.235 total chlorophyll. The results revealed that irrigation
4th cut 4.44. ..42. 5.51. every 10 days and sowing Rhodes grass produced
5th cut 4.411 ..441 5.525 highest values of total chlorophyll, which was 17.07.
LSD at 5 % 0.070 While, the lowest values of total chlorophyll were
produced from irrigation every 20 days and sowing
Concerning the interaction between cuttings and Blue panic grass (11.67).
forage species on forage dry matter yield/fed, the
results in Table (9) clearly indicated that this

Kandil and Shareif


Page 48
Int. J. Agri. Agri. R.

Table 10. Averages of total chlorophyll (SPAD) as With respect to the interaction effect between
affected by the interaction between irrigation irrigation intervals and forage species on number of
intervals and forage species, as average of three cuts, stems/m2, the results in Table (13) clearly showed
during 2013/2014 and 2015 seasons. that this interaction significantly affected number of
Irrigation Forage species stems/m2. The results indicated that irrigation every
intervals Alfalfa Rhodes grass Blue panic grass 10 days and sowing Rhodes grass produced highest
Every 10 days 16.60 17.07 14.61
Every 20 days 14.84 12.53 11.67 number of stems/m2, which was 450.0 stems/m2.
LSD at 5 % 0.18 Whilst, the lowest number of stems/m2 was produced
from irrigation every 20 days and sowing alfalfa
Concerning the interaction between irrigation
(107.0 stems/m2).
intervals and forage species on leaf area/plant (cm2),
the results in Table (11) clearly showed that this
Table 13. Averages of number of stem/m2 as affected
interaction significantly affected leaf area/plant
by the interaction between irrigation intervals and
(cm2). The results indicated that irrigation every 10
forage plant species, as average of three cuts, during
days and sowing Rhodes grass produced highest
2013/2014 and 2015 seasons.
values of leaf area/plant (cm2), which was 25.53
Irrigation Forage species
cm2/plant. While, the lowest values of leaf area/plant
intervals Alfalfa Rhodes grass Blue panic grass
(cm2) was produced from irrigation every 20 days and
Every 10 days 155.5 450.0 349.3
sowing alfalfa (10.45 cm2/ plant).
Every 20 days 107.9 231.0 153.3
LSD at 5 % 0.7
Table 11. Averages of leaf area/plant (cm2) as
affected by the interaction between irrigation
Regarding the interaction effect between irrigation
intervals and forage species, as average of three cuts,
intervals and forage species on forage green yield/fed,
during 2013/2014 and 2015 seasons.
the results in Table (14) clearly showed that this
Irrigation Forage species
intervals Alfalfa Rhodes grass Blue panic grass interaction significantly affected forage green
Every 10 days 16.53 25.53 20.49 yield/fed. The results showed that irrigation every 10
Every 20 days 10.45 15.66 11.12
LSD at 5 % 0.29 days and sowing Rhodes grass produced highest
forage green yield/fed, which was 4.645 t/fed. While,
Regarding the interaction effect between irrigation the lowest forage green yield/fed was obtained from
intervals and forage species on plant height, the irrigation every 20 days and sowing alfalfa (0.514
results in Table (12) clearly showed that this t/fed).
interaction significantly affected plant height. The
results showed that irrigation every 10 days and Table 14. Means of forage green yield (ton/fed) as
sowing Rhodes grass produced tallest plants (84.6 affected by the interaction between irrigation
cm). While, the shortest plants were produced from intervals and forage species, as average of three cuts,
irrigation every 20 days and sowing alfalfa (25.7 cm). during 2013/2014 and 2015 seasons.
Irrigation Forage species
Table 12. Means of plant height (cm) as affected by intervals Alfalfa Rhodes grass Blue panic grass
the interaction between irrigation intervals and forage Every 10 days 1.768 4.645 3.520
species, as average of three cuts, during 2013/2014 Every 20 days 0.514 1.588 0.892
and 2015 seasons.
LSD at 5 % 0.044
Irrigation Forage species
intervals Alfalfa Rhodes grass Blue panic grass
Every 10 days 50.1 84.6 66.3 With respect to the interaction effect between
Every 20 days 25.7 47.3 26.2 irrigation intervals and forage species on forage dry
LSD at 5 % 0.49
matter yield/fed, the results in Table (15) clearly

Kandil and Shareif


Page 49
Int. J. Agri. Agri. R.

showed that this interaction significantly affected Table 17. Average of crude protein percentage as
forage dry matter yield/fed. The results revealed that affected by the interaction between irrigation
irrigation every 10 days and sowing Rhodes grass intervals and forage species, as average of three cuts,
produced highest forage dry matter yield/fed, which during 2013/2014 and 2015 seasons.
was 2.830 t/fed. While, the lowest forage dry matter Irrigation Forage species
yield/fed was produced from irrigation every 20 days intervals Alfalfa Rhodes grass Blue panic grass
Every 10 days 44.4. 44.55 4...1
and sowing alfalfa (0.316 t/fed). Every 20 days .49.14 44.24 4..2.
LSD at 5 % 0.21

Table 15. Average of forage dry matter yield


With respect to the interaction effect between
(ton/fed) as affected by the interaction between
irrigation intervals and crude fiber percentage, the
irrigation intervals and forage species, as average of
results in Table (18) clearly showed that this
three cuts, during 2013/2014 and 2015 seasons.
interaction significantly affected crude fiber
Irrigation Forage species
intervals Alfalfa Rhodes grass Blue panic grass percentage. The results revealed that irrigation every
Every 10 days 1.196 2.830 2.131 20 days and sowing Blue panic grass produced
Every 20 days 0.316 0.996 0.572
LSD at 5 % 0.077 highest crude fiber percentage (30.09%). However,
the lowest crude fiber percentage was produced from
With respect to the interaction effect between irrigation every 10 days and sowing alfalfa (16.42%).
irrigation intervals and forage species on water use
efficiency, the results in Table (16) clearly indicated Table 18. Average of crude fiber percentage as
that this interaction significantly affected forage water affected by the interaction between irrigation
use efficiency. The results revealed that irrigation intervals and forage species, as average of three cuts,
every 10 days and sowing Alfalfa produced highest during 2013/2014 and 2015 seasons.
values of water use efficiency (4.49). While, the lowest Irrigation Forage species
forage dry matter yield/fed was produced from intervals Alfalfa Rhodes grass Blue panic grass
Every 10 days 44.15 51.1. 59.11
irrigation every 20 days and sowing alfalfa (1.06). Every 20 days 41.11 54..4 .1.19
LSD at 5 % 0.13
Table 16. Average of water use efficiency as affected
Concerning to the interaction effect between
by the interaction between irrigation intervals and
irrigation intervals and forage species on ether
forage species, as average of three cuts, during
extract, the results in Table (19) clearly showed that
2013/2014 and 2015 seasons.
this interaction significantly affected ether extract.
Irrigation Forage species
intervals Alfalfa Rhodes grass Blue panic grass The results revealed that irrigation every 10 days and
Every 10 days 1.19 ..41 ..41 sowing Alfalfa produced highest ether extract
Every 20 days 4.14 4.44 4.44
LSD at 5 % 0.04 (2.40%). While, the lowest ether extract was produced
from irrigation every 10 days and sowing Blue panic
Regarding to the interaction effect between irrigation grass (1.63%).
intervals and forage species on crude protein
percentage, the results in Table (17) clearly showed Table 19. Average of ether extract as affected by the
that this interaction significantly affected crude interaction between irrigation intervals and forage
protein percentage. The results revealed that species, as average of three cuts, during 2013/2014
irrigation every 20 days and sowing Alfalfa produced and 2015 seasons.
highest crude protein percentage (19.41%). which was Irrigation Forage species
2.792 t/fed. While, the lowest crude protein intervals Alfalfa Rhodes grass Blue panic grass
Every 10 days 5.11 5.11 4.4.
percentage was produced from irrigation every 10 Every 20 days 5..1 5.55 4.14
days and sowing Rhodes grass (11.22%). LSD at 5 % 0.06

Kandil and Shareif


Page 50
Int. J. Agri. Agri. R.

With respect to the interaction effect between 318.2 Kg/fed. While, the lowest crude protein
irrigation intervals and forage species on ash yield/fed was produced from irrigation every 20 days
percentage, the results in Table (20) clearly showed and sowing alfalfa (44.1 Kg/fed).
that this interaction significantly affected ash
percentage. The results revealed that irrigation every Concerning to the interaction effect between
51 days and sowing Rhodes grass produced highest irrigation intervals and forage species on crude fiber
ash percentage Blue panic grass (1.63%). While, the yield/fed, the results in Table (23) clearly showed that
lowest ash percentage was produced from irrigation this interaction significantly affected crude fiber
every 20 days and sowing alfalfa (10.78%). yield/fed. The results discovered that irrigation every
10 days and sowing Rhodes grass produced highest
Table 20. Average of ash percentage as affected by crude fiber yield/fed, which was 701.8 Kg/fed. While,
the interaction between irrigation intervals and forage the lowest crude fiber yield/fed was produced from
species, as average of three cuts, during 2013/2014 irrigation every 20 days and sowing alfalfa (52.1
and 2015 seasons. Kg/fed).
Irrigation Forage species
intervals Alfalfa Rhodes grass Blue panic grass Table 22. Average of crude protein yield/fed as
Every 10 days 41.14 44.44 44.42
Every 20 days 41.44 44..4 44.41 affected by the interaction between irrigation
LSD at 5 % 0.07 intervals and forage species, as average of three cuts,
during 2013/2014 and 2015 seasons.
Regarding to the interaction effect between irrigation
Irrigation Forage species
intervals and forage species on nitrogen free extract
intervals Alfalfa Rhodes grass Blue panic grass
percentage, the results in Table (21) clearly showed
Every 10 days 552.5 .44.5 514.4
that this interaction significantly affected nitrogen
Every 20 days 44.1 441.4 11.2
free extract percentage. The results revealed that
LSD at 5 % 5.8
irrigation every 10 days and sowing Rhodes grass
produced highest nitrogen free extract percentage
Table 23. Average crude fiber yield/fed as affected
(51.29%). While, the lowest forage dry matter
by the interaction between irrigation intervals and
yield/fed was produced from irrigation every 20 days
forage species, as average of three cuts, during
and sowing alfalfa (44.79%).
2013/2014 and 2015 seasons.

Table 21. Average of nitrogen free extract percentage Irrigation Forage species

as affected by the interaction between irrigation intervals Alfalfa Rhodes grass Blue panic grass

intervals and forage species, as average of three cuts, Every 10 days 494.1 114.4 449.1

during 2013/2014 and 2015 seasons. Every 20 days 25.4 545.1 415.1
LSD at 5 % 12.6
Irrigation Forage species
intervals Alfalfa Rhodes grass Blue panic grass
Every 10 days 19.11 24.59 11.92
Every 20 days 14.92 19.44 11.19 Regarding to the interaction effect between irrigation
LSD at 5 % 0.60 intervals and forage species on ether extract yield/fed,
the results in Table (24) clearly showed that this
With respect to the interaction effect between
interaction significantly affected ether extract
irrigation intervals and forage species on crude
yield/fed. The results revealed that irrigation every 10
protein yield/fed, the results in Table (22) clearly
days and sowing Rhodes grass produced highest ether
showed that this interaction significantly affected
extract yield/fed, which was 57.3 Kg/fed. While, the
crude protein yield/fed. The results revealed that
lowest ether extract yield/fed was produced from
irrigation every 10 days and sowing Rhodes grass
irrigation every 20 days and sowing alfalfa (7.4
produced highest crude protein yield/fed, which was
Kg/fed).

Kandil and Shareif


Page 51
Int. J. Agri. Agri. R.

Table 24. Average of ether extract yield/fed as chlorophyll (SPAD), leaf area/plant (cm2), plant
affected by the interaction between irrigation height (cm) and number of stems/m2, the results in
intervals and forage species, as average of three cuts, Table (1) clearly showed that this interaction
during 2013/2014 and 2015 seasons. insignificantly affected total chlorophyll, leaf
Irrigation Forage species area/plant (cm2), plant height (cm) and number of
intervals Alfalfa Rhodes grass Blue panic grass stems/m2.
Every 10 days 54.1 21.4 ...4
Every 20 days 1.1 55.. 41.1 Regarding the interaction effect between cuttings,
LSD at 5 % 2.7 irrigation intervals and forage species on forage green
yield/fed, the results in Table (27) clearly revealed

With respect to the interaction effect between that this interaction significantly affected forage green

irrigation intervals and forage species on ash yield/fed. The results showed that highest forage

yield/fed, the results in Table (25) clearly showed that green yield/fed was produced from the fifth cut of

this interaction significantly affected ash yield/fed. Rhodes grass when irrigated every 10 days, which was

The results revealed that irrigation every 10 days and 4.735 t/fed. On contrary, the lowest forage green

sowing Rhodes grass produced highest ash yield/fed, yield/fed was produced from the first cut and sowing

which was 296.8 Kg/fed. While, the lowest ash alfalfa when irrigated every 20 days, which was 0.490

yield/fed was produced from irrigation every 20 days t/fed.

and sowing alfalfa (40.6 Kg/fed).


Table 26. Average of nitrogen free extract yield/fed

Table 25. Average of ash yield/fed as affected by the as affected by the interaction between irrigation

interaction between irrigation intervals and forage intervals and forage species, as average of three cuts,

species, as average of three cuts, during 2013/2014 during 2013/2014 and 2015 seasons.

and 2015 seasons. Irrigation Forage species

Irrigation Forage species intervals Alfalfa Rhodes grass Blue panic grass

intervals Alfalfa Rhodes grass Blue panic grass Every 10 days 214.9 4121.1 954.1

Every 10 days 42..1 594.4 544.1 Every 20 days 421.5 141.4 522.9

Every 20 days 11.4 412.5 41.5 LSD at 5 % 7.8

LSD at 5 % 4.2
With respect to the interaction effect between

Regarding to the interaction effect between irrigation cuttings, irrigation intervals and forage species on

intervals and forage species on nitrogen free extract forage dry matter yield/fed, the results in Table (1)

yield/fed, the results in Table (26) clearly showed that clearly indicated that this interaction insignificantly

this interaction significantly affected nitrogen free affected forage dry matter yield/fed.

extract yield/fed. The results revealed that irrigation


every 10 days and sowing Rhodes grass produced Regarding to the interaction effect between cuttings,

highest nitrogen free extract yield/fed, which was irrigation intervals and forage species on percentages

4121.1 Kg/fed. While, the lowest nitrogen free extract of water use efficiency, crude protein and fiber, ether

yield/fed was produced from irrigation every 20 days extract, ash and nitrogen free extract as well as crude

and sowing alfalfa (421.5 Kg/fed). protein and fiber, ether extract, ash and nitrogen free
extract yield/fed during 2013/2014 and 2015 seasons,

Interaction between cuttings, irrigation intervals the results in Tables (2 and 3) indicated that this

and forage species effect interaction insignificantly affected these traits during

Concerning to the interaction effect between cuttings, 2013/2014 and 2015 seasons.

irrigation intervals and forage species on total

Kandil and Shareif


Page 52
Int. J. Agri. Agri. R.

Table 27. Averages of forage green yield (ton/fed) as affected by the interaction between cuttings, irrigation
intervals and forage species during 2013/2014 and 2015 seasons.
Irrigation intervals
Every 10 days Every 20 days
Cuttings
Forage species Forage species
Alfalfa Rhodes grass Blue panic grass Alfalfa Rhodes grass Blue panic grass
1st cut 1.641 4.424 3.322 0.490 1.616 0.909
2nd cut 1.781 4.639 3.487 0.517 1.578 0.886
3rd cut 1.796 4.707 3.583 0.518 1.577 0.887
4th cut 4.41. 1.155 ..294 1.25. 4.242 1.491
5th cut 4.441 1.1.2 ..441 1.25. 4.242 1.491
LSD at 5 % 1.199

Potassium nitrate concentrations foliar application application of KNO3 significantly reduced the
effect increasing tendency of Na+ and Cl- and increased leaf
With respect to effect of potassium nitrate area, its fresh and dry weight per plant, NO3- and
concentrations foliar application on total chlorophyll soluble protein concentration and NR activity in
(SPAD), leaf area (cm2), plant height (cm), number of leaves irrespective to the growth of plant under non
stems/m2, forage green and forage dry matter saline or saline conditions. Furthermore, conditions.
yield/fed., the results in Table (1) showed that foliar Gimeno et al. (2014) concluded that a 2% foliar KNO3
application of potassium nitrate at different application can enhance the tolerance of citrus plants
concentrations significantly affected total chlorophyll to water stress by increasing the osmotic adjustment
(SPAD), leaf area (cm2), plant height (cm), number of process. High salt concentrations in the root zone
stems/m2, fresh and dry forage yield ton/fed. The causes adverse effects on plant. These salt effects may
results showed that foliar spraying of potassium cause membrane disorganization, production of toxic
nitrate at 15 ppm significantly increased total metabolites and inhibition in photoshynsis leading to
chlorophyll (SPAD), leaf area (cm2), plant height reduced growth of plants. Foliar fertilization can
(cm), number of stems/m2, forage green and forage therefore be a complementary measure taken to
dry matter yield/fed. The corresponding data were provide nutrients during a critical phase of restricted
14.89, 17.39, 51.03, 242.2, 2.228 and 1.410, nutrient supply. In This Respect, Kaya et al. (2007)
respectively. However, the lowest values of total found that exogenously applied potassium nitrate
chlorophyll (SPAD), leaf area (cm2), plant height through the root zone significantly mitigated the
(cm), number of stems/m2, forage green and forage adverse effects of salt stress on growth of melon. They
dry matter yield/fed were obtained from without added that the alleviating effects of potassium nitrate
potassium nitrate foliar spraying i.e. the control were attributed to maintenance of membrane
treatment, which were 13.91, 15.89, 49.01, 233.6, permeability and enhanced concentrations of Ca2+, N
2.076 and 1.281, respectively. Akram and Ashraf and K+ in the leaves of salt stressed plants.
(2009) concluded that exogenous application of
potassium nitrate increased the photosynthetic rate With respect to results presented in Tables (2 and 3)
and leaf turgor which in turn resulted enhanced results clearly indicated a significant effect due to
growth and yield in sunflower plants subjected to salt foliar application of potassium nitrate on percentages
stress. Foliar application of KNO3 was found to of water use efficiency, crude protein and fiber,
increase nitrate content of ryegrass leaves under non- extracting ether, ash and nitrogen free extract and
saline or saline condition (Tabatabaei and Fakhrzad, nitrogen free extract as well as crude fiber, extracting
2008). Jabeen and Ahmed (2011) found that ether, ash and nitrogen free extract yield/fed during

Kandil and Shareif


Page 53
Int. J. Agri. Agri. R.

2013/2014 and 2015 seasons. Highest percentages of Table 28. Averages of forage green yield/fed as
water use efficiency, crude protein, crude fiber and affected by the interaction between cuttings and
ether extract, and nitrogen free extract and nitrogen potassium nitrate concentrations foliar spraying
free extract as well as crude fiber, extracting ether, during 2013/2014 and 2015 seasons.
ash and nitrogen free extract yield/fed during Potassium nitrate concentrations
Cuttings
2013/2014 and 2015 seasons. The correspond data 0 ppm 10 ppm 15 ppm
were 2.41,14.99, 24.54, 2.19, 11.76 % as well as 191.2, 1st cut 2.004 2.065 2.131
353.1, 29.3,157.2 and 655.4 Kg/fed, respectively 2nd cut 2.061 2.154 2.229
during 2013/2014 and 2015 seasons. In addition, the 3rd cut 2.098 2.186 2.250
lowest of above traits were produced from without 4th cut 5.412 5.492 5.529
foliar application of potassium nitrate during 5th cut 5.441 5.51. 5.549
2013/2014 and 2015 seasons. However highest LSD at 5 % 0.072
percentages of nitrogen free extract (49.29%) was
produced from without foliar application of Regarding the interaction effect between cuttings and
potassium nitrate during 2013/2014 and 2015 potassium nitrate concentrations foliar application on
seasons. Akram and Ashraf (2009) reported that forage dry matter yield/fed, the results in Table (1)
exogenous application of KNo3 can increased the indicated that this interaction insignificantly affected
photosynthetic rate and leaf turgor which in turn forage dry matter yield/fed.
resulted in enhanced growth and yield in sunflower
plants subjected to salt stress. Foliar applied KNO3 With respect to the interaction effect between cuttings
increased leaf N and K+ and regulated the opening and foliar application of potassium nitrate on
and closing of stomata thereby maintaining plant percentages of water use efficiency, crude protein and
water efficiency. fiber, ether extract, ash and nitrogen free extract as
well as crude protein and fiber, ether extract, ash and
Interaction between cuttings and potassium nitrate nitrogen free extract yield/fed during 2013/2014 and
concentrations foliar application effect 2015 seasons, the results in Tables (2 and 3) indicated
Concerning to the interaction effect between cuttings that this interaction insignificantly affected these
and potassium nitrate concentrations foliar traits during 2013/2014 and 2015 seasons.
application on total chlorophyll, leaf area/plant (cm2),
plant height (cm) and number of stems/m2, the Interaction between irrigation intervals and
results in Table (1) clearly indicated that this potassium nitrate foliar application effect
interaction insignificantly affected total chlorophyll, Regarding the interaction effect between irrigation
leaf area/plant (cm2), plant height (cm) and number intervals and potassium nitrate concentrations foliar
of stems/m2. application on total chlorophyll, the results in Table
(29) indicated that this interaction significantly
Regarding the interaction effect between cuttings and affected total chlorophyll. Highest values of total
potassium nitrate concentrations foliar application on chlorophyll were produced from irrigation every 10
forage green yield/fed, the results in Table (28) days and potassium nitrate foliar application at 15
indicated that this interaction significantly affected ppm (44.1). However, the lowest values of total
forage green yield/fed. Highest forage green yield/fed chlorophyll were produced from irrigation every 20
was produced from the fifth cut and potassium nitrate days and without potassium nitrate foliar application
foliar application at 15 ppm (2.269 t/fed). However, (12.4).
the lowest forage green yield/fed was produced from
the first cut and without potassium nitrate foliar
application (2.004 t/fed).

Kandil and Shareif


Page 54
Int. J. Agri. Agri. R.

Table 29. Averages of total chlorophyll as affected by Table 31. Means of plant height as affected by the
the interaction between irrigation intervals and interaction between irrigation intervals and
potassium nitrate concentrations, as average of three potassium nitrate concentrations, as average of three
cuts, during 2013/2014 and 2015 seasons. cuts, during 2013/2014 and 2015 seasons.
Irrigation Potassium nitrate concentrations Irrigation Potassium nitrate concentrations
intervals 0 ppm 10 ppm 15 ppm intervals 0 ppm 10 ppm 15 ppm
Every 10 days 15.74 16.14 16.40 Every 10 days 65.8 67.2 68.1
Every 20 days 12.40 12.98 13.97 Every 20 days 32.3 33.0 33.9
LSD at 5 % 0.16 LSD at 5 % 0.45

Regarding the interaction effect between irrigation Concerning to the interaction effect between
intervals and potassium nitrate concentrations foliar irrigation intervals and potassium nitrate
application on leaf area (cm2), the results in Table concentrations foliar application on number of
(30) indicated that this interaction significantly stem/m2, the results in Table (32) indicated that this
affected leaf area (cm2). Highest leaf area (cm2) was interaction significantly affected number of stem/m2.
produced from irrigation every 7 days and potassium Highest number of number of stem/m2 was produced
nitrate concentrations foliar application at 15 ppm from irrigation every 10 days and potassium nitrate
(21.77 cm2/plant). However, the lowest leaf foliar application at 15 ppm (319.2 stems /m2).
area/plant (cm2), was produced from irrigation every However, the lowest number of stem/m2 was
20 days and without potassium nitrate foliar produced from irrigation every 20 days and without
application (11.84 cm2/plant). potassium nitrate foliar application (149.3 stems/m2).

Table 30. Averages of leaf area as affected by the Table 32. Averages of number of stem/m2 as
interaction between irrigation intervals and affected by the interaction between irrigation
potassium nitrate concentrations, as average of three intervals and potassium nitrate concentrations, as
cuts, during 2013/2014 and 2015 seasons. average of three cuts, during 2013/2014 and 2015
Irrigation Potassium nitrate concentrations seasons.
intervals 0 ppm 10 ppm 15 ppm Irrigation Potassium nitrate concentrations
Every 10 days 19.95 20.65 21.77 intervals 0 ppm 10 ppm 15 ppm
Every 20 days 11.84 12.40 13.00 Every 10 days 317.8 318.8 319.2
LSD at 5 % 0.20 Every 20 days 149.3 159.8 165.2
LSD at 5 % 0.77
With respect to the interaction effect between
irrigation intervals and potassium nitrate Regarding the interaction effect between irrigation
concentrations foliar application on plant height intervals and potassium nitrate concentrations foliar
(cm), the results in Table (31) indicated that this application on forage green yield/fed, the results in
interaction significantly affected plant height. Tallest Table (33) indicated that this interaction significantly
plants were produced from irrigation every 10 days affected forage green yield/fed. Highest forage green
and potassium nitrate foliar application at 15 ppm yield/fed was produced from irrigation every 10 days
(68.1 cm). However, the shortest plants were and potassium nitrate foliar application at 15 ppm
produced from irrigation every 20 days and without (3.431 t/fed). However, the lowest forage green
potassium nitrate foliar application (32.3 cm). yield/fed was produced from irrigation every 20 days
and without potassium nitrate foliar application
(0.969 t/fed).

Kandil and Shareif


Page 55
Int. J. Agri. Agri. R.

Regarding the interaction effect between irrigation application on total chlorophyll, plant height (cm)
intervals and potassium nitrate concentrations foliar and number of stems/m2, the results in Table (1)
application on forage dry matter yield/fed, the results clearly indicated that this interaction insignificantly
in Table (34) indicated that this interaction affected total chlorophyll, leaf area/plant (cm2), plant
significantly affected forage dry matter yield/fed. height (cm) and number of stems/m2.
Highest forage dry matter yield/fed was produced
from irrigation every 10 days and potassium nitrate With respect to the interaction effect between forage
foliar application at 15 ppm (2.167 t/fed). However, species and potassium nitrate concentrations foliar
the lowest forage dry matter yield/fed was produced application on forage green yield/fed the results in
from irrigation every 20 days and without potassium Table (35) indicated that this interaction significantly
nitrate foliar application (0.605 t/fed). affected forage green yield/fed. The results showed
that highest fresh forage yield/fed was obtained from
Table 33. Averages of forage green yield (ton/fed) as planting Rhodes grass and potassium nitrate foliar
affected by the interaction between irrigation application at 15 ppm (3.195 t/fed). However, the
intervals and potassium nitrate concentrations, as lowest forage green yield/fed was produced from
average of three cuts, during 2013/2014 and 2015 planting alfalfa and without potassium nitrate foliar
seasons. application (1.084 t/fed).
Irrigation Potassium nitrate concentrations
intervals 0 ppm 10 ppm 15 ppm Table 35. Averages of forage green yield (ton/fed) as
Every 10 days 3.183 3.320 3.431
Every 20 days 0.969 0.0014 1.024 affected by the interaction between forage species and
LSD at 5 % 0.046 potassium nitrate concentrations, as average of three
cuts, during 2013/2014 and 2015 seasons.
Table 34. Averages of forage dry matter yield
Potassium nitrate concentrations
ton/fed as affected by the interaction between Forage species
0 ppm 10 ppm 15 ppm
irrigation intervals and potassium nitrate Alfalfa 1.084 1.146 1.193
Rhodes grass 3.022 3.133 3.195
concentrations, as average of three cuts, during Blue panic grass 2.121 2.204 2.294
2013/2014 and 2015 seasons. LSD at 5 % 0.056

Irrigation Potassium nitrate concentrations


intervals 0 ppm 10 ppm 15 ppm Regarding the interaction effect between forage
Every 10 days 1.956 5.1.1 2.167 species and potassium nitrate concentrations foliar
Every 20 days 0.605 0.626 0.653
LSD at 5 % 0.076 application on forage dry matter yield/fed, the results
in Table (1) indicated that this interaction
With respect to the interaction effect between insignificantly affected forage dry matter yield/fed.
irrigation intervals and foliar application of
potassium nitrate on percentages of crude protein With respect to the interaction effect between forage
and fiber, ether extract and nitrogen free extract as species and potassium nitrate concentrations foliar
well as crude protein and fiber, ether extract and application on percentages of crude protein and fiber,
nitrogen free extract yield/fed during 2013/2014 and ether extract and nitrogen free extract as well as crude
2015 seasons, the results in Tables (2 and 3) indicated protein and fiber, ether extract and nitrogen free
that this interaction insignificantly affected these extract yield/fed during 2013/2014 and 2015 seasons,
traits during 2013/2014 and 2015 seasons. the results in Tables (2 and 3) indicated that this
interaction insignificantly affected these traits during
Interaction between forage species and potassium 2013/2014 and 2015 seasons.
nitrate concentrations foliar application effect
Concerning to the interaction effect between forage
species and potassium nitrate concentrations foliar

Kandil and Shareif


Page 56
Int. J. Agri. Agri. R.

Interaction between cutting, irrigation intervals and With respect to the interaction effect between cutting,
potassium nitrate concentrations foliar application effect irrigation intervals and foliar application of
Concerning to the interaction effect between cuttings, potassium nitrate on percentages of water use
irrigation intervals and potassium nitrate efficiency, crude protein and fiber, ether extract and
concentrations foliar application on total chlorophyll, nitrogen free extract as well as crude protein and
leaf area/plant (cm2), plant height (cm) and number fiber, ether extract and nitrogen free extract yield/fed
of stems/m2, the results in Table (1) clearly indicated during 2013/2014 and 2015 seasons, the results in
that this interaction insignificantly affected total Tables (2 and 3) indicated that this interaction
chlorophyll, leaf area/plant (cm2), plant height (cm) insignificantly affected these traits during 2013/2014
and number of stems/m2. and 2015 seasons

Regarding the interaction effect between cuttings, Interaction between Cuttings, Forage Species and
irrigation intervals and potassium nitrate Potassium Nitrate Concentrations Foliar Application
concentrations foliar application on forage green Effect
yield/fed, the results in Table (36) indicated that this With respect to the interaction effect between
interaction significantly affected forage green cuttings, forage species and potassium nitrate
yield/fed. The results revealed that highest forage concentrations foliar application on total chlorophyll,
green yield/fed was produced from the third cut and leaf area/plant (cm2), plant height (cm), number of
irrigation every 10 days and potassium nitrate foliar stems/m2, forage green and forage dry matter
application at 15 ppm (3.251 t/fed). However, the yield/fed, the results in Table (1) clearly indicated that
lowest forage green yield/fed was obtained from the this interaction insignificantly affected total
first cut and irrigation every 20 days and without chlorophyll, leaf area/plant (cm2), plant height (cm),
potassium nitrate foliar application (0.962 t/fed). number of stems/m2, forage green and forage dry
matter yield/fed.
Regarding the interaction effect between cuttings,
irrigation intervals and potassium nitrate With respect to the interaction effect between cutting,
concentrations foliar application on forage dry matter forage species and foliar application of potassium
yield/fed, the results in Table (1), the results indicated nitrate on percentages of water use efficiency, crude
that this interaction insignificantly affected forage dry protein and fiber, ether extract and nitrogen free
matter yield/fed. extract as well as crude protein and fiber, ether
extract and nitrogen free extract yield/fed during
Table 36. Averages of forage green yield (ton/fed) as 2013/2014 and 2015 seasons, the results in Tables (2
affected by the interaction between cuttings, and 3) indicated that this interaction insignificantly
irrigation intervals and potassium nitrate affected these traits during 2013/2014 and 2015
concentrations during 2013/2014 and 2015 seasons. seasons.
Irrigation intervals
Every 10 days Every 20 days Interaction between irrigation intervals, forage
Cuttings Potassium nitrate Potassium nitrate species and potassium nitrate concentrations foliar
concentrations concentrations application effect
0 ppm 10 ppm 15 ppm 0 ppm 10 ppm 15 ppm
Regarding the interaction effect between irrigation
1st cut 3.046 3.136 3.205 0.962 0.995 1.057
intervals, forage species and potassium nitrate
2nd cut 3.156 3.308 3.443 0.966 1.000 1.014
concentrations foliar application on total chlorophyll,
3rd cut 3.228 3.372 3.487 0.969 1.000 1.014
4th cut ..5.1 ...42 ..199 1.91. 4.114 4.149 leaf area/plant (cm2), plant height (cm), number of

5th cut ..514 ...99 ..251 1.91. 4.114 4.149 stems/m2, forage green and forage dry matter

LSD at 5 % 0.011 yield/fed, the results in Table (1) clearly indicated that

Kandil and Shareif


Page 57
Int. J. Agri. Agri. R.

this interaction insignificantly affected total Requirements for Green Fodder Crops in New
chlorophyll, leaf area/plant (cm2) plant height (cm), Reclaimed Lands". Lot of thanks to Agronomy Dept.,
number of stems/m2, forage green and forage dry Faculty of Agriculture for providing help and
matter yield/fed. cooperation during field experiment and chemical
analysis.
With respect to the interaction effect between
irrigation intervals, forage species and foliar References
application of potassium nitrate on percentages of Abusuwar AO, Eldin AK. 2013. Effect of Seed
water use efficiency, crude protein and fiber, ether Pelleting and Water Regime on the Performance of
extract and nitrogen free extract as well as crude Some Forage Species under Arid Conditions.
protein and fiber, ether extract and nitrogen free American-Eurasian Journal Agriculture &
extract yield/fed during 2013/2014 and 2015 seasons, Environment Science 13 (5), 728-734.
the results in Tables (2 and 3) indicated that this
interaction insignificantly affected these traits during Akram MS, Ashraf AM. 2009. Alleviation of
2013/2014 and 2015 seasons adverse effects of salt stress on sunflower Helianthus
annus, L. by exogenous application of potassium
Interaction between cuttings, irrigation intervals, nitrate. Journal of Applied Botany and Food Quality
forage species and potassium nitrate concentrations 83, 19-27.
foliar application effect
Regarding the interaction effect between cuttings, Ali MH, Hoque MR, Hassan AA, Khair A. 2007.
irrigation intervals, forage species and potassium Effects of deficit irrigation on yield, water
nitrate concentrations foliar application on total productivity, and economic returns of wheat.
chlorophyll, leaf area/plant (cm2), plant height (cm), Agricultural water management 92, 151-161.
number of stems/m2, forage green and forage dry
matter yield/fed, the results in Table (1) clearly Al-Soqeer A, Al-Ghumaiz NS. 2012. Studies on
indicated that this interaction insignificantly affected forage yield and feeding value for smegrasses species
total chlorophyll, leaf area/plant (cm2), plant height under different irrigation treatment in Al-Qassium
(cm), number of stems/m2, forage green and forage Region. Journal of Agricultural and Veterinary
dry matter yield/fed. Qassim University 5 (1), 3-16.

With respect to the interaction effect between cutting, Al-Suhaibani NA. 2006. Effect of Irrigation Intervals
irrigation intervals, forage species and foliar and Nitrogen Fertilizer Rates on Fresh Forage Yield of
application of potassium nitrate on percentages of Sudan grass Sorghum sudanense (Piper) Stapf.
water use efficiency, crude protein and fiber, ether Research Bulten Food Science & Agriculture Research
extract and nitrogen free extract as well as crude Center, King Saud Univ 142, 1-14.
protein and fiber, ether extract and nitrogen free
extract yield/fed during 2013/2014 and 2015 seasons, AOAC. 2000. Methods of Analysis of Association of
the results in Tables (2 and 3) indicated that this official Agricultural Chemist. 17th Ed. Washington
interaction insignificantly affected these traits during D.C. USA.
2013/2014 and 2015 seasons.
Bolton ET, McCarthy BJ. 1962. Proc. Nat. Acad.
Acknowledgment Sci., U.S.A 48, 1390–1397.
Authors would like to thank Research Unit of
Mansoura University for funded this works through Gimeno V, Díaz-López L, Simón-Grao S,
funding the project titled "Reducing Irrigation Water Martínez V, Martínez-Nicolás JJ, García-

Kandil and Shareif


Page 58
Int. J. Agri. Agri. R.

Sánchez F. 2014. Foliar potassium nitrate forage sorghum as affected by irrigation interval and
application improves the tolerance of Citrus planting pattern. Advances in Environmental Biology
macrophylla L. seedlings to drought conditions. Plant 6(3), 1298-1303.
Physiology Biochemistry 83, 308-315.
Osman AAM, Abdel Aziz HA, Babiker FSH. 2014.
Gomez AK, Gomez AA. 1991. Statistical procedures A Comparative Study between Rhodes Grass Chloris
for agricultural research. Second Edition, A Wiley gayana Kunth with Local Grass Forages. Universal
Interscience Publication, Jhon Wiley and Sons. Journal of Agricultural Research 2(2), 50-55.

Hashim MAA, Siam N, Al-Dosari A, Asl-Gaadi Sarkar RK, Malik GC. 2001. Effect of foliar spray
KA, Patil VC, Tola EHM, Rangaswamy M, of potassium nitrate and calcium nitrate on grasspea
Samdani MS. 2012. Determination of Water Lathyrus sativus L. grown in rice fallows.
Requirement and Crop water productivity of Crops Department of Agronomy, University College of
Grown in the Makkah Region of Saudi Arabia. Agriculture, Calcutta University, Calcutta, West
Australian Journal of Basic and Applied Sciences Bengal, India pp. 47-48.
6(9), 196-206. Sattar S, Hussnain T, Javaid A. 2010. Effect of
NaCl Salinity on Cotton (Gossypium arboreum L.)
Jabeen N, Ahmed R. 2011. Foliar Application of Grown on MS Medium and in Hydroponic Cultures.
Potassium Nitrate Affects the Growth and Nitrate Journal of Animal and Plant Sciences 20(2), 87 – 89.
Reeducates Activity in Sunflower and Safflower
Sir AM, Ahmed GS. 2014. Effect of Water Stress on
Leaves under Salinity. Notulae Botanicea Horti
Growth and Yield of Alfalfa Medicage stavia L.
Agrobotanici 39(2), 172-178.
Journal of Agriculture-Food and Applied Sciences
2(5), 134-138.
Kamel N, Mohamed MM, Mechliaba NB. 2012.
Impacts of irrigation regimes with saline water on Tabatabaei SJ, Fakhrzad F. 2008. Foliar and soil
carrot productivity and soil salinity. Journal of the application of potassium nitrate affects the tolerance
Saudi Society of Agricultural Sciences 11(1), 19-27. of salinity and canopy growth of perennial ryegrass
Lolium perenne var ‘Boulevard’. American Journal
Kaya C, Levant Tuna A, Ashraf M, Tunlu HAl. Agriculture Biology Sciences 3(3), 544-550.
2007. Improved Salt tolerance of melon Cucumis
melo L. by the addition of proline and potassium Thomas JC, White RH, Vorheis JT, Harris HJ,
nitrate. Environmental Experimental Botany 60, 397- Diehl K. 2006. Environmental impact of irrigating
403. turf with type I recycled water. Agronomy Journal 98,
Koch FC, McMeekin TL. 1924. A new direct 951–961.
nesslerization micro-Kjeldahl method and a
modification of the Nessler-Folin reagent for Zainab M Mobarak, Mahmoud MS, Mohamed
ammonia. Journal American Chemistry Society 46, ME, El-Zanaty AAA. 2013. Improving Growth and
2066-2069. Nutrient Content of Maize and Cotton Plants through
Magnesium Nitrate Foliar Fertilization. American
Mirdad ZM. 2009. Spinach Spinacia oleracea, l.
Journal of Plant Nutrition and Fertilization
Growth and yield responses to irrigation dates,
Technology 3, 22-32.
mineral nitrogen sources and levels application.
Zhu JK. 2002. Salt and drought stress signal
Journal Agricultural & Environmental Sciences
transduction in plants, Annual Review Plant Biology
Alexandria University 8(1), 43-69.
53, 247-273.
Moosavi SGR, Seghatoleslami MJ, Ansari-Nia
E. 2012. Qualitative and morphologic response of

Kandil and Shareif


Page 59

You might also like