Professional Documents
Culture Documents
Period 4
January 9, 2017
Abstract
To investigate the rate of photosynthesis using different colored light, a lab was designed to test
the rate of photosynthesis in a leaf disk. Spinach was used as the type of leaf that will be experimented on.
To discover which set of leaf disks produced oxygen first, a leaf disks was exhausted from its oxygen and
put into a cup of water to sink. Different colored cups were put on top of the water cup to stimulate the
different colored light. The different colored cups included in the lab were: red, yellow, green, and clear.
To find out which colored light had the fastest leaf float- indicating a faster rate of photosynthesis- a timer
was set up to time the leaves. At the end of the lab, the leaves under the yellow light had the fastest time
to float up, because yellow had the closest wavelength to photosynthesis’ peak.
Introduction
Photosynthesis is a process used by plants, bacteria and sometimes algae. Photosynthesis is the
process of absorbing sunlight into a plant’s leaf and using the sunlight as energy to make sugar and
oxygen. All plants and leafs use photosynthesis whether the calvin cycle is in the mesophyll cell or not. In
the calvin cycle, carbon is entered into the cycle and makes sugar through the process in the calvin cycle.
First sun shines on a leaf getting the chlorophyll excited. Then, the chlorophyll gives an electron away
and that electron makes water. The water is broken and the hydrogen goes through the NADP to make
NADPH. Then hydrogen is taken and makes hydrogen and carbon dioxide. There the carbon enters the
If the rate of photosynthesis is being tested using different colored light, then the cup leaves with
the yellow light will have a faster rate of photosynthesis; therefore, having the quickest leaves float,
because yellow light’s wavelength is about 570 nanometers and photosynthesis peaks at about 650
nanometers.
Variables
The clear light was chosen as a control because regularly plants grow in clear light.
Methods
Before the lab, the class had to individually learn how to do the lab as homework. Doing this
prepared the class on how to exactly do the lab effectively and not waste time. As seen from the
homework, the class tried to mimic the procedures of the lab. First the class cleared out our station and the
floor from any backpacks for safety reasons. Next, the following materials were gathered to the group’s
stations: 6 clear cups, 1 yellow cup, 1 green cup, 1 red cup, baking soda (carbon dioxide), a beaker with
water, spinach leaves, straws, 4 syringes, timer, and a light source (materials based on color light focus).
Once the materials were gathered the individual groups proceeded to start their labs based on what each
individual group focused on testing. To test which colored light will have the leaf disks float faster
colored cups were put on top of the cups with leaves in them. To set this up, the group had 4 cups with
water in them and about 3 grams of baking soda in each cup. Then the spinach leaves were cut with the
straws to make the leaf disks. One the group had about 5 disks, the team put the leaf disks in the syringes
with water. Next, all oxygen was taken out of the syringe then a thumb was placed on the tip of the
syringe and the tubing was pulled. Pulling the tube was to take out any oxygen left inside the leaves.
Taking the oxygen outside the leaves will cause the leaf disks to start sinking. Once all disks sank, the
leaves were quickly placed in their designated cups (5 leaf disks for each cup). Once all the 4 cups were
set up, the colored cups were put on top of the cup so that the light is colored when the leaves are heated.
Finally, all 4 cups were put under the light and the light was turned on and the process of photosynthesis
starts. To collect the data, a timer was set up to mark when all leaf disks floated up in the individual cup.
Results
Processed Data
Discussion
This lab was to stimulate which colored light would make the leaf disks float the fastest. Spinach
leaves were used for the experiment on testing the rate of photosynthesis. Baking soda was used for the
carbon dioxide since plants need carbon dioxide, considering it is a limiting factor. The lab showed that
the leaf disks that were under the yellow light floated the fastest. Yellow light was the closest color to
photosynthesis’ peak of wavelength. The leaf disks under the yellow light floated at about 6 minutes and
15 seconds while the next color was green at 8 minutes and 30 seconds for the leaves to float.
Conclusion
To conclude the lab, leaf disks under the yellow light had the faster process of photosynthesis so
that the leaves can create sugar and oxygen to start floating. The control took the longest time with 26
minutes; while, the leaves under the green light came close with the ones under the yellow light with 2
minutes and 15 seconds difference. Although the leaf disks under the green light should have taken the
longest due the its wavelength, there could have been a problem from human error. For example, the
leaves under the green light had warmer water or more carbon dioxide. Having these factors are a major
concern on how fast the leaves can produce oxygen; since water and carbon dioxide are limiting factors to
photosynthesis. Warmer water and more carbon dioxide can speed up the oxygen flow of the leaves.