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29g acrylamide
1g of N,N'-methylbisacrylamide
Dissolve in 60ml of water, adjust the volume to 100ml with water.
Filtration through 0.45 micron pore size filter.
Store at 40C.
Freeze medium
65% glycerol
0.1 M MgSO4
0.025 M Tris-HCl pH 8
1M IPTG
Tryptone, 10 g
Yeast extract, 5 g
NaCl, 10 g
Add distilled water to 950 ml
Adjust the pH to 7.0 with 5 N NaOH.
Adjust the volume to 1 liter with distilled water.
Sterilize by autoclaving.
10M NaOH
Sachin Indian Institute of Technology Guwahati/ Sachin/Protocol‐1
25:24:1 Phenol:Chloroform:Isoamyl-alcohol
25ml Phenol,
24ml Chloroform,
1ml Isoamyl alcohol.
20% SDS
1XSOC
Tryptone, 20 g
Yeast extract, 5 g
NaCl, 0.5 g
add water to 950 ml
3M sodium acetate
Sachin Indian Institute of Technology Guwahati/ Sachin/Protocol‐1
242 g of Tris base
57.1 ml of glacial acetic acid
100 ml of 0.5 M EDTA (pH 8.0)
in 1000ml water.
1M Tris-HCl, pH 6.8
2% X-gal
Sachin Indian Institute of Technology Guwahati/ Sachin/Protocol‐1