Highly Efficient Lentiviral Pluri-
potency Reporters
Features
Higher viral titer than other
lentiviral vectors
Reliable expression levels
Self inactivating vectors
Lentiviral vector design and virus
packing techniques used to create
this product are covered by several
United States patents under agree-
ment to Allele Biotech, contact
vivec@allelebiotech.com or call 858-
587-6645 for details.
Website: www.allelebiotech.com
Call: 1-800-991-RNAi/858-587-6645
(Pacific Time: 9:00AM~5:00PM)
Email: oligo@allelebiotech.com
For Technical Support:
Email:iPS@allelebiotech.com
F or Research Use Only. Not for
Diagnostic or Therapeutic Use.
Purchase does not include or carry any
right to resell or transfer this product
either as a stand-alone product or as a
component of another product. Any use
of this product other than the permit-
ted use without the express written
authorization of Allele Biotech is strictly
prohibited
Allele Biotech-Introducing Cost Effectiveness to Research
Introduction
N
anog, Oct4, and Rex1 promoters have been reported to display ES/iPS cell-
specific expression under undifferentiated state. This kit provides all three
promoter-fluorescent protein (FP) reporters to confirm the state of the reprogrammed
iPSCs. A GFP or RFP gene was cloned behind these ES- specific promoters to offer
convenient fluorescence tracking of undifferentiated cells.
Lentiviral infection has advantages over other gene-delivery methods including:
high-efficiency infection of dividing and non-dividing cells, long- term stable
expression of a transgenes, and low immunogenicity.
Pluripotency Promoter Reporter ABP-SC-ESRKIT1 each reporter 2 vials, 10^7TU in 1 vial
Panel(Nanog, Oct-4 and Rex1) 0.1ml
Nanog Promoter Reporter ABP-SC-RNANOG1 1 vial, 10^7TU in 0.1ml per vial
Nanog Promoter Reporter ABP-SC-RNANOG5 5 vials, 10^7TU in 0.1ml per vial
Oct-4 Promoter Reporter ABP-SC-ROCT4G1 1 vial, 10^7TU in 0.1ml per vial
Oct-4 Promoter Reporter ABP-SC-ROCT4G5 5 vials, 10^7TU in 0.1ml per vial
Rex1 Promoter Reporter ABP-SC-RREX2R1 1 vial, 10^7TU in 0.1ml per vial
Rex1 Promoter Reporter ABP-SC-RREX2R5 5 vials, 10^7TU in 0.1ml per vial
References
1. Da Yong WU, Zhen YAO (2005). Isolation and characterization of the murine
Nanog gene promoter. Cell Research, 15 (5): 317–324.
2. Rachel Eiges, Maya Schuldiner..et.al (2001). Establishment of human embryonic
stem cell-transfected clones carrying a marker for undifferentiated cell. Current
Biology 11: 514–518.
3.Guangjin Pan, Jun Li, Yali Zhou, Hui Zheng, and Duanqing pei (2006). A negative
renewal. ASEB Journal 20: E1094~E1102
Note: The specificity of these promoters are based on scientific publications, which
are subject to debate.
Protocols
Storage: -80oC. Avoid repeated freeze thaw cycles Safety Issues
Lentiviral vectors should be handled using NIH BSL 2
Steps:
safety guidelines. For more information, please see
1. Plate target cells to 90% confluence in a 24-well plate Biosafety in Microbiological and Biomedical Laboratories
before infection. <4th edition> which is available on the Web sites of the
2. Place mWasabi GFP-virus stock (virus titer: 1x10^8 National Institutes of Health at http://bmbl.od.nih.gov.
IU/ml) in a 37°C water bath and before totally thaw The major safety concern about using lentivirus vector is the
transfer onto ice immediately. production of replication competent retrovirus
3. Make a serial dilution of 3-4 different multiplicity of <RCV> in experiment process. The strategy we use to
infection (MOI = infectious units/ cell number) to test decrease the potential risk including:
the target cells. Example 1x10^4 TE671 cells in each We create our vectors by eliminating or modifying ~
well of a 12-well plate:
90% the wild type HIV 1 sequence.
10 MOI 20 MOI 30 MOI 60 MOI None of the structural HIV genes <necessary for
Culture Medium (ul): 397 396 395 392
Polybrene (2mg/ml): 2 2 2 2 production of viral progeny> are present in the
Virus (ul): 1 2 3 6 packaged viral genome.
Total volume (ul): 400 400 400 400 The transducing vector is self inactivating <SIN>.
These SIN vectors have several advantages over wild
4. Tilt the plate gently every 30 min. After 3hrs, add 1 ml type LTR vectors, including reduced transcriptional
fresh medium. interference from the upstream viral promoter and
5. After 48-72 hrs, evaluate mWasabi GFP expression by reduced risk of activating downstream cellular genes
using fluorescence microscope or flow cytometry. upon viral integration.
6. Choose the most efficient MOI with the least toxicity
for iPS cell derivation.

Related Products
Human iPSCs Generation Viral Vectors Human iPSCs Generation Retroviral Particles
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PhoenixTM pCHAC Retroviral Vectors Any individual gene in the set
Human iPSCs Generation Lentiviral Particles Pluripotency Reporter Lentiviral Particles
4-In-One-Vector (OSMK) Set: Nanog, Oct-4 and Rex1
2-In-One-Vector (OS) Any individual promoter in the set
Set: OSKM iPSCs Identification
Set: OSKMP Human iPS RT-PCR Primer Set (50 reactions for
Set: OSKMPT each of 45 genes)
Set: OSNL Mouse iPS RT-PCR Primer Set (50 reactions for
Set: OSNLP each of 29 genes)
Set: OSNLPT Antibodies (Check online)
Pre-Mix: OSNL Irradiated bFGF/LIF Producing Feeder Cells
O: Oct3/4; S: Sox2; K: Klf4; Irradiated bFGF-Producing Human Fibroblasts
M: c-Myc; N: Nanog; L: Lin28 Irradiated bFGF-Producing MEFs
T: Tert; P: p53 shRNA Irradiated bFGF-LIF-Producing MEFs
Any individual gene in the set

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