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0 Introduction
First of all, as for environmental laboratory we had been given a task to come out with
proposal before begin all the lab’s experiment. The objectives of this lab is to analyse the
water quality of selected water resources according to Malaysia Water Quality Index (WQI)
and to determine the suitable application of the water resources. Besides, it is also to identify
the class of the certain area chosen whether it is suitable for any human activities or not.
As a group 3, we had discuss and agreed that we will do the experiment by taking a
sample from FKAAS’s lake. Generally, we can see that this area is one of the favourite area
for fishing activities. In addition, they have a lots of lotus flowers planted in this lake. So, by
conducting the lab experiment from this area’s water sample we are able to know the class of
water and automatically can identify whether it is suitable or safe for fishing activities and
also planting lotus flowers or not in that area.
Moreover, biochemical oxygen demand (BOD, also called biological oxygen demand)
is the amount of dissolved oxygen needed (i.e. demanded) by aerobic biological organisms to
break down organic material present in a given water sample at certain temperature over a
specific time period. The BOD value is most commonly expressed in milligrams of oxygen
consumed per litre of sample during 5 days of incubation at 20 °C and is often used as a
surrogate of the degree of organic pollution of water.
2.0 Methodology
1) Find any location that we are going to conduct an experiment, by taking a water sample
and also consider about human activities around the area.
2) Find polyethylene bottle and wash it using acid nitrate for 1 night or we also just can wash
it 3 times by using the water at the location of sample that need to be taken.
1. Before taking a sample of water, make sure that we are not taking a sample with bubble.
2. In order to avoid or reduce the bubble, we need to take the water's sample slowly.
3. Make sure that the amount of sample water should be enough to conduct all of the
experiment test in lab.
5. Then, continue with the lab experiment by using the sample water taken.
2.3 Laboratory Procedure (According To APHA Standard Any Related Standard)
BLANK SAMPLE
Cr6+
6+
* Oxidizing agent ,Cr
3+
is reduced to Cr
during digestion
* Organic matter are oxidized by
oxidizing agent.
TITARTION
a) Jot down first reading of burette.
b) b. Titrate FAS until the color change from blue – green to reddish brown
c) Stop titration.
d) Jot down last reading.
Compute the Normality (N) for the FAS standard using formula given :
Compute the COD concentration in mg/L for the samples using formula given :
BLANK 1
BLANK2
BLANK3
SAMPLE1
SAMPLE2
SAMPLE3
BOD bottle
2. Measure out the proper volume of sample size and into BOD bottle.
3. Completely fill the BOD bottle with dilution water.
4. For BLANK bottle, completely fill the bottle with only dilution water.
5. Measure the initial DO for each bottles using DO meter.
Dilution water DO meter
Di = initial DO
Df = final DO
Vs = sample volume
Vb = sample bottle volume, 300m
3 or 5 days
2.3.3 Experiment 3: Total Suspended Solid
5. Bacteria counting
a) After being incubate for 1 day, take out the petri plates.
b) Place the petri plate on the counting chamber.
c) At the end of the incubation period, select all of the petri plates containing between 30
and 300 colonies. Plates with more than 300 colonies cannot be counted and are
designated too many to count (TMTC). Plates with fewer than 30 colonies are
designated too few to count (TFTC).
d) Count the colonies on each plate.
2.3.5 Experiment 6: AN, Nitrogen, Ammonia
Before starting :
1. Hold the reagent droppers and dropper bottles vertically, not at an angle, when the
reagent is added.
2. The reagents that are used in this test contain mercury. Collect the reacted samples for
safe disposal.
3. If the Pour-Thru Cell is used, clean the cell periodically. To clean, add several crystals
of sodium thiosulfate pentahydrate into the cell funnel. Add deionized water to
dissolve the crystals. Rinse fully with deionized water.
4. Review the Safety Data Sheets (MSDS/SDS) for the chemicals that are used. Use the
recommended personal protective equipment.
Test Procedure:
1. Start program 380 N, Ammonia, Ness. For information about sample cells, adapters or
light shields, refer to Instrument specific information on page 1.
2. Prepare the sample: Fill a mixing cylinder to the 25mL line with sample.
3. Prepare the blank: Fill a mixing cylinder to the 25‑mL line with deionized water.
4. Add 3 drops of Mineral Stabilizer to each mixing cylinder.
5. Put the stopper on the mixing cylinders. Invert the mixing cylinders several times to
mix.
6. Add 3 drops of Polyvinyl Alcohol Dispersing Agent to each mixing cylinder.
7. Put the stopper on the mixing cylinders. Invert the mixing cylinders several times to
mix.
8. Use a pipet to add 1.0 mL of Nessler Reagent to each mixing cylinder.
9. Put the stopper on the mixing cylinders. Invert the mixing cylinders several times to
mix.
10. Start the instrument timer. A 1-minute reaction time starts.
11. Pour 10 mL from the blank cylinder into a sample cell.
12. When the timer expires,clean the blank sample cell.
13. Insert the blank into the cell holder.
14. Push ZERO. The display shows 0.00 mg/L NH3–N.
15. Pour 10 mL from the sample cylinder into a second sample cell.
16. Clean the prepared sample cell.
17. Insert the prepared sample into the cell holder.
18. Push READ. Results show in mg/L NH3–N.