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Tissue
● Start at 5:40
Brain Basics
● Structure
○ Forebrain (large and complex)
■ Cerebrum - intelligence, memory, personality,
emotion, speech, and ability to feel/move
○ Midbrain
■ Communicates with spinal cord
○ Hindbrain
■ Cerebellum - balance, movement,
and coordination
■ Involuntary functions
Brain Basics Continued
● Tissue:
○ Neurons and axons
■ communication and processing
○ Neuroglia
■ Support and protect neurons
○ Gray matter
■ nerve connections and processing
○ White matter
■ connect gray matter regions
■ information “highway”
Current Research Overview
● How well can we grow brain tissue?
● Current applications?
Current Research: Tufts University
● Structure:
○ Silk scaffold
○ Neurons derived from rats
○ Axons made of gel
● Differentiation
○ 3 weeks
■ midbrain & hindbrain
■ precursors to cerebellum -->
○ 4 weeks
■ top-bottom Purkinje Cell markers (motor movement)
■ deep cerebellar projections (cell migration)
○ 15 weeks 1st
■ rudimentary electrophysiological function
■ cell migration Trimester!
Current Research: Institute of Molecular
Biotechnology in Vienna
● Structure
○ Stem cells from human skin
○ Gel resembling connective tissues
○ Spinning bath to infuse with nutrients
and oxygen
○ 3-4 millimeters after 10 months
(no blood vessels)
● Modeled microcephaly
Current Research: Institute of Molecular
Biotechnology in Vienna
● mini-brains
grown from
stem cells
Ethics
Ethical issues at different phases of stem cell research
Phase of research Ethical issues
Use of stem cell lines derived at another institution Conflicting legal and ethical standards
Informed consent
<http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2726839/table/T1/>
Challenges
● Specific neurons need to be generated
● Neurons need to be able to connect to each
other in specific ways
● Sensory input needed for functional circuits
to form
Future Work/Research
● Growing full sized human brain- unlikely
● Growing brain structures to replace poorly
functioning ones in humans
● Injecting stem cells directly into patient and
let the cells organize themselves
Pathway to industry
● Bioengineering
● Neurobiology
● Developmental Biology
● Biochemistry
● Microbiology
● Biomedical Sciences