Professional Documents
Culture Documents
Agricultural Science
and Technology A
Volume 5, Number 1, January 2015 (Serial Number 43)
David Publishing
D DAVID PUBLISHING
Contents
Research Papers
1 Evaluation and Selection of Date Palm Varieties from Seedling Progeny in Saudi Arabia
Ibrahim Abdoulhadi Al-Abdoulhadi
40 Inclusion Levels of Fermented Apple Bagasse on in Vitro Rumen Fermentation of Alfalfa Hay
Yamicela Castillo-Castillo, Oscar Ruiz-Barrera, Eduviges Burrola-Barraza, Claudio Arzola-Alvarez,
Agustin Corral-Luna, Carlos Rodriguez-Muela and Manuel Murillo-Ortiz
75 Erasing the Asian Longhorned Beetle with Determined Actions, but at High Costs—A Case
Report
Severin Schwendener, Urs Kamm and Markus Hochstrasser
Journal of Agricultural Science and Technology A 5 (2015) 1-7
doi: 10.17265/2161-6256/2015.01.001
D DAVID PUBLISHING
Abstract: A survey was conducted in 2011 in the major date growing regions of Saudi Arabia to identify and evaluate date palms
with fruit characteristics preferred by the producers and consumers. The regions in the Kingdom surveyed for this purpose were
Al-Zulfi, Al-Hassa, Najran, Al-Madinah Al-Munawarah and Al-Qassim. Seventy palms from seedlings were initially identified for
the study based on the following fruit characteristics, i.e., fruit length, breadth, weight, color, taste, texture, sugar content, external
appearance and also the market value. Subsequently, 12 palms (Al-Afiah, Duhiba, Adbah, Aliah, Shamshula, Masifat Al-Asedy,
Suwaid, Batlyah, Al-Nassar, Hussan, Sukariyat Al-Zulfi and Al-Hamat) mainly from the Zulfi and Riyadh regions were found to
meet the required standards with potential for commercial date farming. Among these, fruits of Adbah had the highest total sugars of
72.9% followed by Duhiba with a total sugar content of 71.9%. Fruit length was maximum (49.1 mm) in the variety Aliah, while the
fruits of the variety Hussan recorded the maximum weight of 19.5 g. Furthermore, it is pertinent to mention that fruit size of the 12
selected cultivars was generally large and comparable to Majdool, a well-known date palm cultivar of North Africa. The fruits of the
selected cultivars were also tasty and can be stored separately.
Key words: Fruit characteristics, Phoenix dactylifera, seedling date palm, Saudi Arabia.
Abstract: Rain water harvesting practices and their effects on the productivity of farming systems in the semi-arid areas of Nigeria
have been investigated using participatory rural appraisal (PRA) technique. There was no direct water harvesting interventions by
government agencies in the area. Farmers take advantage of naturally existing depressions and abandoned burrow pits close to their
farmlands to harvest rain water for surface irrigation using petrol engine pumps. The reservoirs surveyed were found to be grossly
inadequate in the drought period. Water budget analysis revealed that evaporation and seepage losses from reservoirs were high in
both agro-ecological zone considered. The application of rain water harvesting practices was found to have increased farmers income
by 61% and 125% in the Sahel and Sudan Savannah agro-ecological zones, respectively. Governments at all levels in these areas
need to support these farmers in order to boost their production to achieve food security in these drought prone areas. Farmers lack
timely access to farm credit and agricultural inputs; the indigenous water harvesting practices identified need to be enhanced with the
aid of agricultural engineering extension services.
Key words: Rainfall, water harvesting, irrigation, drought, productivity, farming systems.
Abstract: Studies have provided indirect evidence that cellulolytic activity of some anaerobic bacteria is repressed by carbohydrates,
such as glucose. This effect is known as carbon catabolite repression (CCR). Previous work has found that cellulolytic activity of
Clostridium cellulovorans and Eubacterium cellulosolvens are regulated. Many cellulolytic systems of these organisms are expressed
only in the presence of cellulose or cellobiose (the disaccharide of cellulose). Some of these cellulose-induced systems also appear
subject to CCR when more soluble substrates, such as glucose, are also available. To determine if such repression directly effects
cellulolytic activity of C. cellulovorans and E. cellulosolvens, these organisms were cultivated in media containing a glucose analog.
We then measured the ability of low levels of analog to inhibit growth of the organisms when cellobiose or cellulose were the energy
substrates. Our results found that growth of both C. cellulovorans and E. cellulosolvens in cellobiose-containing medium are strongly
inhibited by glucose analogs. In addition, both organisms exhibited delayed and slower growth in cellulose-containing medium when
a glucose analog was added. These results provide direct demonstration that these cellulolytic bacteria are subject to CCR. This
repression of cellulolysis may affect both of these organisms’ ability to serve as industrial platforms for biomass degradation, and
may interfere with the contribution of E. cellulosolvens toward animal digestion of cellulose. These results were also in sharp
contrast to what has been reported regarding CCR activity in Clostridium cellulolyticum, which actively expresses cellulases in the
presence of low levels of glucose.
Key words: Cellulolytic, anaerobic bacteria, catabolite repression, cellulolysis, cellulose degradation.
Abstract: Transport of nonreactive solutes in soils is principally controlled by soil properties, such as particle-size distribution and
pore geometry. Surface tension of soil water yields capillary forces that bind the water in the soil pores. Changes in soil water surface
tension by contaminants may affect flow of soil water due to decreased capillary forces, caused by lowered soil water surface tension.
This study aimed at assessing solute transport in sand columns as affected by effluent surface tension. Miscible displacement (MD)
tests were conducted on sand columns repacked with sands sieved from 2.0, 1.0, 0.5 and 0.25 mm screens. The MD tests were
conducted with 0.05 M bromide solutions prepared using water with surface tension adjusted to 72.8, 64, 53.5 and 42 dyne/cm2.
Obtained breakthrough curves were modeled with the convection-dispersion equation (CDE) model. Coefficient of hydrodynamic
dispersion and pore-water velocity responded inconsistently across decreased particle-sizes and water surface tensions and this was
attributed to non-uniform effect of lowered effluent surface tension on solute transport in different pore-size distribution.
Key words: Breakthrough curve, hydrodynamic dispersion, convection dispersion equation, pore-water velocity, miscible
displacement.
Nataliya Vladimirovna Punina1, Mikhail Anatolievich Remnev2 and Alexey Fedorovich Topunov1
1. A. N. Bach Institute of Biochemistry RAS, Moscow 119071, Russia
2. The Federal State Unitary Enterprise All-Russia Research Institute of Automatics, Moscow 127055, Russia
Abstract: Rapid and reliable diagnostics and identification of pathogenic and symbiotic bacteria are at the top of the agenda. In the
first case, they are important to control and prevent crop damages, and thus reduce economic losses. In the second, it’s necessary to
design and monitor quality of biofertilizer to raise its effectiveness and crop capacity. Development of accurately, rapidly, technically
and commercially accessible methods remains a critical problem for the bacteria with comprehensive phylogenetic structure. In this
work, we investigated pathogenic Xanthomonas and Ralstonia and symbiotic Sinorhizobium. The aim of this investigation was to
examine the applicability of the novel methods for phylogenetic study, identification and diagnostics of closely related species of
these genera. The conventional phenotypic and genotypic (16S rRNA, gyrB) methods were applied as referents. Novel polymerase
chain reaction (PCR)-based approaches, single-adapter amplified fragment length polymorphism (saAFLP) and comparative analyses
of hin-region and Xcc0006-0007 sequences, were first employed for the investigations. Phenotypic tests, 16S rRNA and gyrB
analysis distinguished bacteria at the genus level, but failed to identify them to the species robustly. The new methods identified
bacteria at the inter-species level more precisely. This identification agreed with the accepted genera’s classifications. The only
exceptions were X. fuscans & X. citri and X. perforance & X. euvesicatoria which clustered together. The further outcome of this
study was achieved hin-region-based genus-specific PCR primers for the express-diagnostics of the genera. Summary, these new
methods can be applied for genome-based phylogeny investigations and as convenient and accurate tools for identification and
routine laboratory diagnostics of these comprehensive genera.
Abstract: The aim of this study was to assess the effect of inclusion of fermented apple bagasse (FAB) obtained through solid state
fermentation on pH, ammonia nitrogen (N-NH3), volatile fatty acids (VFA) content, in vitro dry matter digestibility (IVDMD), lactic
acid and microbial counting of alfalfa hay under in vitro rumen environment; four levels of FAB were evaluated (0, 0.25, 0.50 and 0.
75 g/dry matter of FAB) replacing 1.5 g dry matter (DM) of alfalfa hay and incubated at different fermentation times (0, 4, 8, 12 and
24 h) using a complete random design with repeated measures on time. Counts of live yeast colonies (6.08, 6.33, 6.24 and 6.51
CFU/mL expressed as log 10) was higher when FAB was included in the different levels up to the 12 h of fermentation (P < 0.0001);
lactic acid content also increased as FAB was included in the different levels (10.61, 13.86, 16.84 and 14.57 µg/mL) up to the 12 h of
incubation (P < 0.001). Nevertheless, the other variables measured as pH, N-NH3, VFA, IVDMD, total bacteria and fungi counts,
were not affected by the treatments. It is concluded that substitution of FAB by alfalfa hay in an in vitro rumen ecosystem positively
modified live yeast colonies and lactic acid concentration, without effect on the other fermentative and microbial parameters of the in
vitro rumen environment, but considering mixes of FAB and alfalfa hay as a quality ingredient for the feeding of ruminants.
Eliakunda Kimbi1, Faustin Lekule2, James Mlangwa3, Helena Mejer4 and Stig Thamsborg4
1. Tanzania Livestock Research Institute (TALIRI), Uyole, P.O. Box 6191, Mbeya 53126, Tanzania
2. Department of Animal Science and Production, Sokoine University of Agriculture, P.O. Box 3004, Morogoro 67125, Tanzania
3. Department of Veterinary Medicine and Public Health, Sokoine University of Agriculture, P.O. Box 3021, Morogoro 67125,
Tanzania
4. Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Dyrlaegevej 100,
DK-1870 Frederiksberg C, Denmark
Abstract: Pig production is becoming increasingly important in Eastern Africa in recent years. Two studies were carried out in
Mbozi and Mbeya rural districts to characterise smallholder pig production systems and associated pig management practises. A
participatory rural appraisal (PRA), involving 279 pigs-keeping households in nine villages and a cross sectional survey using a
random sample of 300 pig farmers in 30 villages of the study districts, were used to collect data. Important economic activities were
crop farming, livestock keeping, petty business, casual labour and artisan works in that order. The mean farm size and number of pigs
per household was 2 ± 2.7 ha and 4.9 ± 4.2 pigs, respectively. The largest categories of pig herd structure were breeding females
followed by piglets. Age, marital status, household size and land size of household head had significant influence on the pig herd size
(P < 0.05). Pig farmers practised three main types of pig management systems, namely, total confinement, semi confinement and free
range/herding. Each system had specific management practices in relation to feeding and housing. The frequencies of these systems
varied significantly (P < 0.01) by socio-economic factors, such as, educational status of household head, land size and herd sizes of
pigs, goats and cattle. The authors recommend effective use of locally available resources and careful consideration of existing
farming systems for sustainable development of smallholder pig production.
Anja Gräff1, Renate Luise Dörfler1, Manfred Höld2, Jörn Stumpenhausen2 and Heinz Bernhardt1
1. Technische Universität München, Center of Life and Food Sciences, Agricultural Systems Engineering, Am Staudengarten 2,
D-85354 Freising-Weihenstephan, Germany
2. Hochschule Weihenstephan-Triesdorf, University of Applied Sciences Weihenstephan-Triesdorf, Faculty of Agricultural and
Nutritional Sciences, Am Hofgarten 1, D-85354 Freising-Weihenstephan, Germany
Abstract: As there are increasing numbers of small farms in Germany, it is necessary for them, to keep the workload as small as
possible by enhancing the use of automatism. Important as those energetic-technical capabilities and features in a modern dairy farm
are, the final decision when using automatic machinery depends on the animal itself and its behavior. As a result, all
animal-physiological criteria, animal protection and animal welfare have to be taken into consideration. Therefore tests have been
done to investigate dairy cattle behavior on suddenly occurring energy failures, fluctuations or postponements due to a load
management. The experiments have been taken in four different stables. In each stable, 12 “focus cows” have been selected. Their
daily stress response was measured by a heart rate monitor and faecal cortisol metabolites. Video observation showed modifications
in behavior, escape or avoidance reactions; pedometers recorded the movement activity. These scientific experiments will
demonstrate cattle behavior in situations driven by a power load management, but no statistically significant effects on the usual daily
routine are being expected.
Key words: Cattle behavior, cortisol, heart rate variability, power load management, smart grid, Germany.
Abstract: The profitability of broiler chicken production depends on many interrelated factors, such as the size of the farm, farming
system, the genetic potential, feed price and the quality of chicken meat obtained as a final product, e.g., meat, and a strategy of their
sales on the domestic and foreign market. Economic analysis were conducted in randomly selected farms growing commercial
broilers, from April 2008 to March 2013 (28 cycles all together), at the same time in three buildings, with 46 thousands to 54
thousands one-day chickens. In the analyzed period, broilers were reared for 35-50 d. The average slaughter body mass ranged from
2.36 kg to 2.99 kg with feed conversion ratio (FCR) 1.68 kg of feed/kg of body weight gain to 1.78 kg of feed/kg of body weight gain.
Stock mortality at that time ranged from 3.5% to 6.4%. On the basis of macroeconomic data, a clear relationship between the
increase in the period particularly from 2004 onwards, prices of feed 33.6% and the purchase price of livestock 22.5% in the case of
the evaluated farms were found. These values were 27.9% and 31.7%, respectively. From the analysis of the relationship between
different types of broiler production costs, it was concluded that the main determinant of the profitability of live poultry production
was the cost of feed and first of all was the prices of feed. This means that the boom in live poultry production is variable, resulting in
consequences for production and income.
Abstract: In 2012, an infestation of Asian Longhorned Beetle (ALB) was detected in Winterthur (Canton Zurich, Switzerland). With
more than 140 adult beetles and several hundred eggs and larvae, the infestation was by far the most extensive in Switzerland. Based
on long-term experiences made in Braunau (Austria), where the first ALB infestation in Europe occured, the beetle was fought with
extensive actions: removal of all infested trees, preventive removal of potential host trees and installation of a focus- and a buffer
zone. Transport of plant material out of these zones was banned and a close monitoring regime including specialised dogs put in
place. As a result of these efforts, there were no adult beetles and only a few living larvae detected 2013. Neither larvae nor beetles
were found in 2014. However, costs are high: 700,000 Swiss francs were spent in 2012 alone, not including hundreds of working
hours spent by employees of the Canton Zurich. The previewed monitoring programme between 2013 and 2017 is estimated to cost
another 2.8 million Swiss francs, if no more living beetles are detected. The experiences from Winterthur thus show that elimination
of a relatively small ALB infestation with determined actions is likely possible in short time. However, the high costs demonstrate
the need for strong boarder controls or alternative packaging materials for imports from infested regions (China). In addition, a
concept for small communities with limited resources is needed.
Key words: Infestation, zone, focus, buffer, transport, monitoring, elimination, resources.