Professional Documents
Culture Documents
Submitted by:
Kate Pamela T. Bialen
Researcher
Page |2
Researcher:
Kate Pamela T. Bialen
ABSTRACT
RESEARCH PLAN
Tools:
Incubator, mortar and pestle, alcohol lamp,
refrigerator, beakers, test tubes, cheese cloth, and amber
bottles.
B. Treatments / General Procedure
Extraction Procedure
1. Pound mg of Agavaceae plants separately using
mortar and pestle.
2. Squeeze, strain and filter the pounded leaves of
Buntot-tigre into a beaker with the use of the
cheese cloth. The same procedure implies with the
pounded leaves of Tigre. This procedure is repeated
until the desired amount of extract is acquired.
Treatments:
Treatment A= 100% combined Agavaceae plant extracts
Treatment B= 75% combined Agavaceae plat extracts and
25% distilled water
Treatment C= 50% combined Agavaceae plant extracts and
50% distilled water
Treatment D= Positive control (chloramphenicol)
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Procedure:
2. Bioassay Proper
Mueller-Hinton agar plates and test plates in 4
replicates were divided in to four equal parts each by
means of using a marking pen and were respectively
labeled.
All plates were heavily streaked with the bacterial
suspension of pseudomonas aeruginosa. The four
replicates of the four replicates of the streaked
Mueller-Hinton agar plates. The four parts were then
applied with the corresponding treatments with the use
of a dropper. The same procedure was used with the 3
other replicates. These plates were allowed to stay at
room temperature for a few minutes, then these plates
were incubated at 37 C for 24 hours.
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C. Procedural Design
Preparation of
I. Title Page 1
II. Abstract 2
Research Plan 3
Pr o c e d u r a l d e s i g n 4
I I I . In t r o d u c t i o n 8
Ba c k g r o u nd o f t h e Study 8
St a t e m e n t o f t h e problem 8
Objectives 9
Definition of Terms 10
VII. Plates 22
VIII. Reference/Bibliography 27
IX. Appendices 28
X. Acknowledgements 32
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INTRODUCTION
A. Background of the Study
D. OBJECTIVES
To determine the antibacteriall activity of the
Agavaceae family.
To determine which specie of Agavaceae is most
effective as an antibacterial.
E. SIGNIFICANCE OF THE STUDY
G. Definition of terms
Chloramphenicol- Chloramphenicol is a man-made
antibiotic. It slows growth of bacteria by preventing
them from producing important proteins that they need
to survive.
- it refers to the positive control usesd in
the study
Mueller- Hinton Agar-is a medium very rich in
nutrients that was originally recommended for the
isolation and development of gonococci and
meningococci. It is used primarily for sensitivity
testing of microorganisms.
Agavaceae- the flowering plant order Asparagales,
consisting of 23 genera and 637 species of short-
stemmed, often woody plants distributed throughout
tropical, subtropical, and temperate areas of the
world. Members of the family have narrow, lance-
shaped, sometimes fleshy or toothed leaves that are
clustered at the base of each plant. Most species have
large flower clusters containing many flowers. The
fruit is a capsule or berry.
- In this study, buntot-tigre (Cordyline
roxburghiana (Schultes) Merr.) and Tigre
(Sansevieria trifasciata Prain) was used in
the study.
P a g e | 11
Pseudomonas Aeruginosa
Characteristics
Buntot-tigre
Tigre
R1 R2 R3 R4 Mean
A 30 20 30 35 28.75
B 25 25 25 28 25.75
C 15 18 13 18 16
Positive
15 18 14 20 16.75
Control
Results show that the Treatment A got the highest mean among the
4 treatments with 28.75 followed by Treatment B with the mean of
25.75, Treatment D or the Positive control with 16.75 and the
treatment C with the mean of 16.
P a g e | 19
ANOVA
Inhibition
Sum of Df Mean F Sig.
Squares Square
Between
427.500 3 142.500 13.959 .000
Groups
Within
122.500 12 10.208
Groups
Total 550.000 15
CONCLUSION
Based on the executed experimentation, the researcher
therefore concludes that:
The constituents of the agavaceae species are
effective as an antibacterial against Pseudomas
Aeruginosa.
There are significant differences in the mean zones of
inhibition on Pseudomas Aeruginosa using the different
concentrations of the combined extracts of the
agavaceae species.
P a g e | 21
RECOMMENDATIONS
Based on the conclusion and results, the following
proposals are given:
1. Supplementary study about the utilization of the other
parts of the plants used in this study.
2. Supplementary studies are recommended regarding the use of
the similar plants.
3. Additional studies should be conducted if the agavaceae
species have an antibacterial effect on other bacteria.
P a g e | 22
PLATES
Putting of holes unto the agar plate for the extract to fully
penetrate.
Application of treatments.
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Incubation
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REFERENCE/ BIBLIOGRAPHY
A. Website
http://www.stuartxchange.com/Tigre.html
http://www.stuartxchange.com/Buntot-tigre.html
B. Books
APPENDICES
A. Statistical Tool
ANOVA
Inhibition
Sum of df Mean F Sig.
Squares Square
Between
427.500 3 142.500 13.959 .000
Groups
Within Groups 122.500 12 10.208
Total 550.000 15
Multiple Comparisons
Dependent Variable: Inhibition
Tukey HSD
B. Time table
DA T E TASK REMARKS
evaluated and th e
researcher mad e
some corrections
Ju l y 2 1 , 2 0 1 4 C o r rection of the The task was don e
s i gnificance of
the study,
s t atement of the
problem, the
hypothesis
objectives,
scopes and
limitations
Ju l y 2 8 , 2 0 1 4 A p proval of the The task was done
s i gnificance of
the study,
s t atement of the
problem, the
hypothesis
objectives,
scopes and
limitations
Au g u s t 1 5, 2 0 1 4 P r eparation and The task was done
g a thering of the
tools and
m a terials needed
in the
e x perimentation
Au g u s t 1 6 , 2 0 1 4 A pplication of The task was done
extracts.
Au g u s t 1 7 , 2 0 1 4 The task was done
M e asuring of the
zones of
P a g e | 31
inhibition
Au g u s t 2 5, 2 0 1 4 P hytochemical The task was done
a n alysis of the
p lant samples.
Se p t e m b e r 1, 2 0 1 4 Analysis and The task was done
c o nstruction of
the acquired
data.
Se p t e m b e r 1 0 , F i nalization of The document wa s
20 1 4 t he write ups. submitted to be
corrected.
Se p t e m b e r 1 3 , R e vision of the The task was
20 1 4 write ups. done.
Se p t e m b e r 1 6 , A p proval of the The task was
20 1 4 write ups. done.
P a g e | 32
ACKNOWLEDGEMENT