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ABSTRACT
Acetic acid, CH3COOH is an organic compound that is in form of colourless solution and
classified as a weak acid. The aim of this experiment is to determine the morality of a solution
and to study the percent by mass of acetic acid in vinegar by titration with the standardized
sodium hydroxide solution. By performing titration process, the concentration of acetic acid in
vinegar can be determined by calculating both molarity ad percent by mass of acetic acid in
vinegar itself. The experiment is divided into two parts, first part is standardizing the NaOH
solution and the second part is proceeded with the determining the molarity of a solution and
the percent by mass of acetic acid in vinegar. In this experiment, approximately morality of
1M NaOH solution was already prepared by lab assistant. This NaOH solution is then titrated
with potassium hydrogen phthalate or KHP solution which has been prepared by diluting 1.5g
of KHP granules in 30mL of distilled water. This experiment is then proceeded to the second
part of the experiment which standardized NaOH solution is titrated with the 10mL vinegar
that has been diluted with 100mL of distilled water. Titration for part 1 were repeated twice so
that more accurate result can be determined. From equivalence point, volume of NaOH to
neutralized vinegar solution can be determined. By undergo a few steps of calculation, the
percent by mass of acetic acid is calculated.
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2. INTRODUCTION
Molarity (M) = (Equation 1-2)
Percent by mass is the mass in grams of solute per 100 grams of solution.
Percent solute = 100% (Equation 1-3)
Vinegar is a dilute solution of acetic acid. Percent volume of acetic acid in vinegar is between
range of 5% to 20%. Both molarity and percent by mass of acetic acid in a vinegar solution can
be determine by performing a titration. A titration is a process in which small increments of a
solution of known concentration are added to a specific volume of a solution of unknown
concentration until the stoichiometry for that reaction is attained and equivalence point is
reached. The equivalence point is reached when the added quantity of one reactant is the exact
amount necessary for stoichiometry reaction with another reactant. By adding NaOH, which is
basic solution, to the acetic acid, which is and acidic solution, a neutralization will occur.
Before the experiment begins, solution of sodium hydroxide that will be used need to be
standardised first to determine its concentration. This is done by titrating sodium hydroxide
solution with stable acidic compound of high purity which can be accurately prepared to be
known a concentration. In this experiment, we will use potassium hydrogen phthalate (KHP)
for this purpose.
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3. OBJECTIVES
a) To determine accurately the morality of a solution and the percent by mass of acetic
acid in vinegar by titration with the standardized strong base sodium hydroxide
solution.
4. THEORY
Acid-Base titrations are usually used to find the amount of a known acidic or basic substance
through acid base reactions. The analyte is the solution with an unknown molarity. The reagent
is the solution with a known molarity that will react with analyte. In the titration process, a
burette is used to dispense a small, quantifiable increment of solution of known concentration
(Figure 1.1a). A typical burette has the smallest calibration unit of 0.1mL (Figure 1.1b),
therefore volume dispense from the burette tip should can be estimated to the nearest 0.01mL.
Figure 1.1: a) Depicts a typical 50-mL burette. b) Indicates smallest calibration unit,
0.1mL on a typical 50 mL burette.
In this experiment, the equivalence point occurs when the moles of acid in the solution equals
to the moles of base added in the titration. For example, the stoichiometric amount of 1 mole
of the strong base, sodium hydroxide (NaOH), is necessary to neutralize 1 mole of the weak
acid, acetic acid (CH3CO2H) as indicated in equation.
The sudden change in pH of the solution shows that the titration has reached the equivalence
point. pH in an aqueous solution is related to its hydrogen ion concentration. The hydrogen ion
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concentration is written as [H3O+]. pH is defined as the negative of the logarithm of the
hydrogen ion concentration.
Figure 1.2: shows example of acid-base titration of CH3COOH titrated with NaOH
curve graph.
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5. MATERIALS AND APPARATUS
1M NaOH solution, 1.5g of KHP, 10mL of vinegar, distilled water, 50mL burette, 500mL
beaker, 250mL conical flask, 10mL volumetric pipette, burette stand, stirrer, weighing
balance and pH meter.
6. EXPERIMENTAL PROCEDURES
a) 250mL of approximately 1M NaOH were prepared in the beaker and filled in the burette.
b) An empty 250ml beaker have been weighed to the nearest 0.001g. Weight have been
recorded. Then 1.5g of KHP have been added in the beaker.
c) Beaker with KHP inside have been weighted again to the nearest 0.001g. Mass of KHP have
been recorded by the differences in previous data.
d) Then, 30mL of distilled water were added to the beaker filled with KHP. The solution was
stirred until KHP dissolved completely.
e) This solution then was titrated with NaOH with 1mL addition each and pH data was
recorded.
f) Steps a to e then were repeated to perform a second trial to standardize the NaOH solution.
g) All data were recorded. Graph pH versus NaOH was plotted then volume of NaOH required
to neutralize KHP solution in each titration were determined.
h) Molarity and average molarity of NaOH for titraton 1 and 2 have been calculated.
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6.2 Molarity of acetic acid and percent of vinegar
a) 10mL of vinegar were transferred to a clean, dry 250mL beaker using a 10mL volumetric
pipette. 100mL of distilled water were added to the vinegar solution to cover pH electrode tip.
b) Titration were conducted by adding 1mL each of NaOH to the solution. pH data were
recorded.
c) Steps a and b then were repeated to perform a second trial for this experiment.
d) Graph pH vs volume of NaOH were plotted. Volume of NaOH required to neutralize vinegar
solution has been determined and data were recorded.
e) Molarity and average molarity of acetic acid in vinegar for titration 1 and 2 has been
calculated.
f) Percent by mass of acetic acid in vinegar for titration 1 and 2 has been calculated.
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7. RESULTS AND CALCULATIONS
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KHP titrated with NaOH Trial 1
14
12
10
pH reading
6
10.6mL NaOH at
4 the equivalence
2
point
0
0 2 4 6 8 10 12 14 16
Volume of NaOH (mL)
Graph shows data plotted for KHP titrated with NaOH Trial 1
12
10
pH reading
6 10.5mL NaOH at
4
the equivalence
point
2
0
0 2 4 6 8 10 12 14 16
Volume of NaOH (mL)
Graph shows data plotted for KHP titrated with NaOH Trial 2
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-Molarity of NaOH solution
1
10.6mL NaOH x = 0.0106L NaOH
1000
0.007399
Molarity = = = 0.6980M NaOH
0.0106
0.007346
Molarity = = = 0.6996M NaOH
0.0105
Molarity for Trial 1 = 0.6980M NaOH, Molarity for Trial 2 = 0.6996M NaOH
0.6980M+ 0.6996M
= 0.6988M NaOH
2
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7.2 Molarity of acetic acid and percent of vinegar
7.2-1 Volume of NaOH required to neutralize vinegar.
Titration 1 Trial Titration 2 Trial
Volume of NaOH required 17.55 17.60
to neutralize vinegar (mL)
10
Vinegar titrated with NaOH Trial 1
14
12
10
pH reading
4
17.55mL NaOH at the
2 equivalence point
0
0 4 8 12 16 20 24
Volume of NaOH (mL)
Graph shows data plotted for Vinegar titrated with NaOH Trial 1
12
10
pH reading
4
17.60mL NaOH at the
2 equivalence point
0
0 4 8 12 16 20 24
Volume of NaOH (mL)
Graph shows data plotted for Vinegar titrated with NaOH Trial 2
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- Moles of CH3COOH neutralized by moles of NaOH
1 3
0.01227mol NaOH x = 0.01227mol CH3COOH
1
Molarity for Trial 1 = 1.227M CH3COOH, Molarity for Trial 2 = 1.230M CH3COOH
1.227M+ 1.230M
= 1.2285M CH3COOH
2
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7.2-5 Percent % of mass of acetic acid in vinegar for titration 1 and 2.
Percent by mass for Trial 1 = 7.368% CH3COOH, Percent by mass for Trial 2 = 7.386%
CH3COOH
7.368%+7.386%
= 7.377% CH3COOH
2
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8. DISCUSSION
The results show in every 10mL of vinegar solution contains average 0.7377g CH3COOH acid
approximately and have 7.377% of mass percent each. Adding 100mL of distilled water to
10mL vinegar solution did not change the mole number of acid, just to dilute and to cover pH
meter electrode tip. Logically, the results will not be 100% accurate because inaccurately
measurement could easily have been made without professional equipment in way to determine
the molarity of the sodium hydroxide.
Besides that, the way to determine the equivalence point in every graph also not really 100%
accurate. The equivalence point might be slightly greater than 7 because if titration occurs
between weak acid and strong base, the solution produced is weakly alkaline. The product
which is CH3COONa will dissociates to produce conjugate base and reacts with water to
produce hydroxide ions (H+). Thus, the equivalence point will not always be 7 for some
reactions. It will be higher or lower than 7 instead. End point for all of this experiment also
hard to determine because there is no indicator used such as phenolphthalein, but can be known
as vertical section of the curve.
The significance of percent by mass and molarity of solution in this experiment tells whether
the solution either diluted or concentrated solution. Hence, we know the acetic acid in vinegar
is diluted solution due to its percent by mass and molarity is relatively small.
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9. CONCLUSION
10. RECOMMENDATIONS
There are some recommendations for this experiment. First the pH meter that we used must to
be calibrated first in order to get more accurate reading during immersed in solution. Then, we
must aware there is no bubble in the burette tip since NaOH is filled inside. Parallax error must
be avoided when the reading is taken at the burette measuring scale. To get more accurate and
precise data, the series of trial must be repeated at least twice or thrice. Indicator such as
phenolphthalein also can be suggested to indicate an end point in each experiment.
11. REFERENCES
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12. APPENDICES
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