Professional Documents
Culture Documents
Please fashion preliminary answers to the following questions before our class meeting
on Friday 10/13/17 and email me your responses (rmyers@miami.edu); this will help
direct your reading of the paper. Write your answers directly into this Pre-Discussion
File following each question; do not modify the file in any other way. Save your answers
to a file named exactly like this:
Send this file as an attachment to rmyers@miami.edu with the following subject heading
(exactly as written below):
After the meeting on 10/20/17, revisit your answers and modify them to suit your
evolved understanding. Mark your altered text using yellow highlights. Return these re-
answered questions via email to rmyers@miami.edu before 5 PM on Sunday 10/22/17.
Save the second set of answers as before, except replace Pre-Discussion with Post-
Discussion. Also send your email with the subject as I said before except again replace
Pre-Discussion with Post-Discussion. I want two sets of answers from you. If your
understanding remains the same after the discussion, submit your answers again with
the appropriate change to the file name. I use this information to see how your thinking
is altered by the in-class discussion.
Q3: What experimental techniques were used to carry out the plan in Q2?
- Red Recombinase system, immunofluorescence, phylogenetic analysis
and sequence alignment.
Q4: Why did the authors choose to use multiple E. coli UPEC isolates,
Caco-2 colorectal carcinoma cells, female C3H/HeN and female C57BL/6
mice? What unique features led the investigators to use these organisms? What
experimental limits did this choice of research material create?
- Eliminate male aggression, to examine effects of treatment across a wide
spectrum, treatment might be different in males due to higher
testosterone in males.
Q6: What topic or question do you think the authors will take up next based
on this paper? What are the important next questions raised?
Can this affect the natural E.coli present in the digestive system.
Q7: Identify three different experiments that the authors used to come to the
conclusion you stated in answer to Question 5. State what you perceive to be a
limitation to one or more of these experiments that might weaken their
conclusions.
Q8: How do pili promote higher levels of UPEC in the gut? How do they
contribute (if at all) to UTIs?
Pili are adhesive and allow the bacteria to attach (better?, not sure what
word to use) to the lining of the digestive tract.
Q9: What are the implications the restricted phylogenetic distribution of
F17-like pili genes in E. coli?
-E.coli would be less able to cling to digestive lining (no adhesive protein
expression in pili)