To Cover Page

SECTION 2 HYDRAULICS

2.1 PNEUMATIC SYSTEM......................................................... 1

2.2 USE OF PRESSURE AND VACUUM ................................... 2
2.3 HYDRAULIC SYSTEM ......................................................... 3
2.4 ANALYSIS PARAMETERS................................................... 4
2.5 ANALYSIS FLOW ................................................................ 5
2.6 FCM DETECTOR BLOCK .................................................... 6
2.6.1 FUNCTION ....................................................................... 7
2.6.2 OPTICAL SYSTEM........................................................... 9
2.7 HYDRAULIC PARTS ...........................................................10
2.7.1 SOLENOID VALVE FUNCTION..................................... 10
2.7.2 HYDRAULIC PARTS LOCATION .................................. 11
2.8 HYDRAULIC SEQUENCE DESCRIPTION ..........................17

UF-1000i S/M June 2006

SECTION 2 HYDRAULICS

2.1 PNEUMATIC SYSTEM

The system to provide the required pressure and vacuum for main unit operation consists of the following. Pressurized air is regulated at 0.26
MPa by a relief valve. An air drier is used to remove any remaining moisture before it is supplied to the main unit. The pressure of the dry air is
regulated at 0.05 MPa by .Relief Valve.
The source vacuum from pneumatic unit is adjusted to 0.053 MPa by the bellows unit in the main unit.
A filter is provided on the bellows unit to prevent the needle in the bellow from accumulating dirt.
The outline of pneumatic control system is shown below.

Figure 2-1: UF-1000i Pneumatic System

UF-1000i S/M 2-1 June 2006

2.2 USE OF PRESSURE AND VACUUM

Use of pressure and vacuum for the main unit are as follows.

Type Name Functions

Pressure 0.26 MPa Driving master valves, air cylinders,
0.05 MPa Drain of Waste Chamber-2,
Driving Diaphragm Pumps
Sheath Sheath Flow forming for FCM
Pressure
Vacuum -0.053 MPa Replenishment of reagent and driving
diaphragm pumps.
Drain of Waste Chamber-1

UF-1000i S/M 2-2 June 2006

2.3 HYDRAULIC SYSTEM

SED/BAC Block Diagram

UF-1000i S/M 2-3 1 Revised September 2006

2.4 ANALYSIS PARAMETERS

Analysis parameters
5 analysis parameters
(1) RBC (Red Blood Cells)
(2) WBC (White Blood Cells)
(3) EC (epithelial cells)
(4) CAST
(5) BACT (Bacteria)
Flagging Parameters
(6) Path. CAST (CAST with inclusion)
(7) MUCUS
(8) SRC (Small Round Cells)
(9) XTAL (crystals)
(10) YLC (Yeast-Like Cells)
(11) SPERM (spermatozoa)
(12) RBC-Info. (RBC Information)

Analysis
SED BAC
Block
Principle FCM
Parameter RBC WBC EC CAST BACT
S_FLLW S_FLLW
S_SFC S_SFC B_FSC
Information 2 2
S_FLH S_FLL B_FSCW
SFLLW SFLLW
UFPACK-SED UFPACK-BAC
Reagent
UFSEARCH-SED UFSEARCH-BA

UF-1000i S/M 2-4 June 2006

2.5 ANALYSIS FLOW
Sample
Aspiration
Diluent Stain Probe Diluent Stain

Volumetric Volumetric Volumetric Volumetric

SRV SRV
Syringe Syringe Syringe Syringe
435 L Asp. 15 L Asp. 150 L Asp. 12.5 L Asp. 12.5 L Asp. 425 L Asp.
Disp. Disp. Disp. Disp. Disp. Disp.
Reaction Unit
Temperature Control, Mixing, Dilution, Stain
Conductivity Sheath Sheath
Detector Chamber Chamber

Sample Sample
Syringe Syringe

Sheath Flow Cell Red Light Semiconductor Laser

Registance FSC Sensitive SSC Sensitive SFL Sensitive

Amplification Circuit Element Element Element

Sensitivity
Correction Analysis Unit

Conductivity SC Signal SFL Signal

Resistance Signal
Value SSC Pulse Height SFL Signal Height
Resistance Pulse
SSC Pulse Width SFL Pulse Width

Micro Computor

Result Display

UF-1000i S/M 2-5 June 2006

 2.6 FCM DETECTOR BLOCK

Optical Unit No.22 NOTE:

PCB No. 2161T1 (Incl. PCB NO. 2159T1) PMT
Laser Diode is very sensitive to static electricity. Follow the
instruction below when you replace the Laser Diode.
-To prevent electrostatic discharge damage, touch the chassis
to establish a ground before servicing Optical Unit No.22.
- Remove jumper pin from the terminal on the Laser Diode
Pin Hole AFTER connecting to PCB No. 2161. If the jumper pin is
removed before connecting to PCB No.2161, Laser Diode
will be damaged.
- Do not disconnect the connector to the PCB No.2161.
In the worst case, the device inside the Laser Diode will be
destroyed.
Before reapplying the power, be sure that VR-1 on PCB 2161
Optical Unit No.23 is turned all the way CCW.
(Incl. PCB NO.2159) Collector Flow Cell
Lense Laser Diode

T o PCB N o .2 1 6 1

Jumpe r P in

Lase r D iode

The life of the Laser Diode is approx. 10,000 hours.

UF-1000i S/M 2-6 June 2006

2.6.1 FUNCTION
(1)Outline
Laser diode (Wave length: 635 nm) apply to samples in
sheath flow generated flow cell. FCM Detector Block detects
Forward scattered light, side scattered light, and side
fluorescence light from these samples. Uf-1000i generates
two-dimensional scattergrams.
(2) Beam spot generating system
Beam spot generating system consists of laser diode, collimator
lens. Laser beam generated by laser diode is converted to parallel
beam by collimator lens. This beam is collected by condenser lens
and applies to samples. Beam shape is as follows.
(5) Dichroic mirror
Light below 640 nm wave length reflects to the side scattered
light PMT.
(6) Side fluorescence light system
Side fluorescence light system consists of interference filter,
PMT (Photo Multiplier Tube). The light through dichroic mirror
is filtered by interference filter and fluorescence light over 660
nm wave length is detected by PMT. Detected fluorescence
light signal is amplified by pre-amplifier and transmitted to
main amplifier.

10 m
(7) Side Scattered light system
Side Scattered light system consists of ND filter (Neutral
220 m Density filter) and PMT. The light through dichroic mirror is
attenuated by ND filter and scattered light is detected by
(3) Sheath flow system photo diode. Detected scattered light signal is amplified with
Sheath flow is generated in the flow cell. Sample flows center pre-amplifier and transmitted to main amplifier.
of the flow cell.

(4) Forward scattered light system

Forward scattered light system consists of beam stopper and
FSC condenser lens. Beam stopper shade direct light from
laser diode, FSC condenser lens collect the scattered light
from the sample particles.

UF-1000i S/M 2-7 June 2006

 (8) Temperature Control
FCM Detector block is heater controlled to maintain the optical
stability.

(9) Cover switch

Cover switch is to prevent interference of external light and
leakage of the laser beam. If the cover is opened, UF-1000i
stops laser emission and an error massage is displayed.

UF-1000i S/M 2-8 June 2006

2.6.2 OPTICAL SYSTEM
Beam Spot Generator System

Side Fluorescence Light System

PMT

Interference Filter

Dichroic NDFilter (Nuetral Density Filter )

Mirror Photo Diode

Condenser lens Side Scattered Light System

Laser Diode
Pin Hole
Beam Stopper

Condenser Lens
Colimeter Condenser
Lens Sheath Flow System Front Scattered Light System
Lens

Beam Spot Generator System

UF-1000i S/M 2-9 June 2006

2.7 HYDRAULIC PARTS
2.7.1 SOLENOID VALVE FUNCTION
SV No Function Located Unit Page SV No. Function Located Unit Page
1 BAC dilution DP operate Valve Unit 183 Assy 2-15 25 WC1 Drain Valve Unit 185 Assy 2-15
2 Switching liquid path between Valve Unit 183 Assy 2-15 26 Remove sheath heater bubble Valve Unit 185 Assy 2-15
Cellclean and distilled water for
Flow Cell cleaning
3 Flow Cell Rinse line IN Valve Unit 183 Assy 2-15 27 Rinse w-urine pump Valve Unit 185 Assy 2-15
4 SED dil DP operate Valve Unit 183 Assy 2-15 28 High speed sheath Valve Unit 185 Assy 2-15
5 29 Remove sheath block bubbles Valve Unit 185 Assy 2-15
6 Rinse mixing line Valve Unit 183 Assy 2-15 30 Low speed sheath IN Valve Unit 185 Assy 2-15
7 Dispense sheath in Rinse cup Valve Unit 183 Assy 2-15 31 Rinse charging pump Valve Unit 185 Assy 2-15
8 Flow Cell Rinse line OUT Valve Unit 183 Assy 2-15 32 Air Filter waste Drain Valve Unit 186 Assy 2-15
9 Depriming Sheath Block for Valve Unit 183 Assy 2-15 33
Shipping
10 Charging WC1 open Valve Unit 183 Assy 2-15 34 RINSE Chamber Replenish Valve Unit 186 Assy 2-15
11 Charging open Valve Unit 183 Assy 2-15 35 FCM Chamber Replenish Valve Unit 186 Assy 2-15
12 36 WC2 Drain Valve Unit 186 Assy 2-15
13 37 BAC dye DP operate Valve Unit 187 Assy 2-16
14 SED sample flow open Valve Unit 185 Assy 2-15 38 SED dye DP operate Valve Unit 187 Assy 2-16
15 BAC sample flow open Valve Unit 185 Assy 2-15 39 Sample mixing air blow Valve Unit 184 Assy 2-15
16 Open BAC diluent nozzle dispense Valve Unit 185 Assy 2-15 40 Mix sample bubble Valve Unit 184 Assy 2-15
17 Rinse nozzle by BAC diluent Valve Unit 185 Assy 2-15 41 Mixing DP Operate Valve Unit 184 Assy 2-15
18 ED diluent dispense line Valve Unit 185 Assy 2-15 42 Blood Collection Tube detection Valve Unit 184 Assy 2-15
19 Dispense sheath into SED reaction Valve Unit 185 Assy 2-15 43 SRV rotate Valve Unit 184 Assy 2-15
unit
20 BAC diluent dispense line Valve Unit 185 Assy 2-15 44 Pinch SED reaction unit Valve Unit 184 Assy 2-15
21 Dispense sheath into BAC reaction Valve Unit 185 Assy 2-15 45 Pinch BAC reaction unit Valve Unit 184 Assy 2-15
unit
22 SED reaction unit drain Valve Unit 185 Assy 2-15 46 Pinch rinse cup drain Valve Unit 184 Assy 2-15
23 Rinse sheath syringe Valve Unit 185 Assy 2-15 47 Remove rinse cup droplet Valve Unit 184 Assy 2-15
24 BAC reaction unit drain Valve Unit 185 Assy 2-15 48

UF-1000i S/M 2-10 June 2006

 VALVE UNIT NO.
2.7.2 HYDRAULIC PARTS LOCATION 187 ASSY BELLOWS UNIT
TRAP CHAMBER
VALVE UNIT NO.
184 ASSY

REGUATOR
BAC
(0.05MPa) 1
Reaction Unit
SED
Reaction Unit REGULATOR 1
(Sheath Pressure)

SHEATH
BLOCK

SHEATH
SYRINGE
ASSY 1
Trap Chambers
AIR BUILT-IN
SRV DRIER PNEUMATIC
UNIT

UF-1000i S/M 2-11 1 Revised September 2006

 SHEATH BCK VALVE NO.183 ASSY CHAMBER UNIT
SHEATH CHAMBER RINSE CHAMBER

VALVE NO.186 ASSY

WASTE CHAMBER 1
WASTE CHAMBER 2

VALVE NO.185
ASSY

LEAKAGE DETECTION
SENSOR

UF-1000i S/M 2-12 June 2006

WHOLE URINE PUMP and CHARGING PUMP 1

WHOLE URINE PUMP 1

CHARGING PUMP 1

UF-1000i S/M 2-13 1 Revised September 2006

 SRV Move
Pos. Sensor SRV Initial
Pos. Sensor

Sample Filter Assembly

UF-1000i S/M 2-14 June 2006

(1) VALVE NO.183 ASSY (3) VALVE NO.185 ASSY

10 4 3
8 2
6 1
11 9
7 4 3
2 1

SED REAGENT PUMP

(585.0L)
BAC REAGENT PUMP
(487.5L)

(4) VALVE NO.186 ASSY

(2) VALVE NO.184 ASSY

UF-1000i S/M 2-15 June 2006

(5) VALVE NO.187 ASSY (5) SHEATH SYRINGE ASSY

37
38

BAC STAIN PUMP

(12.5L) 1
SED STAIN PUMP
(15L)

UF-1000i S/M 2-16 1 Revised September 2006

2.8 HYDRAULIC SEQUENCE DESCRIPTION
0.0 -5.2 sec.

SRV rotates to
MOVE position for
4.1 17.6
seconds.

Sample aspiration probe

ascend to the initialize
position for 4.0 - 5.2
seconds..

UF-1000i S/M 2-17 June 2006

0.0 -5.2 sec.
(1) The whole urine syringe descends to aspirate 800L (1200L for Sampler Mode) of urine sample for 0.2 - 3.1 seconds.
(2) Sample aspiration probe ascend to the initialize position for 4.0 - 5.2 seconds.
(3) SRV rotates to the move position by activating SV43 for 4.1 - 17.6 seconds.
(4) SED reaction chamber is drained by activating SV22 for 4.1 - 4.5 sec.

UF-1000i S/M 2-18 June 2006

5.2 15.0 sec.

SRV is at MOVE
position for 4.1
17.6 seconds.

Sample aspiration probe

moves to above rinse
cup 12.1 -13.3 sec.,
and descends into rinse
cup 12.1 13.3
seconds.

UF-1000i S/M 2-19 June 2006

5.2 15.0 sec.
(5) Mixing rod for both of SED & BACT reaction chambers starts rotation by activating its DC motor for 5.3 - 14.9 seconds.
(6) 435L of SED diluent and 150L of whole urine (585L in total) are dispensed into SED reaction chamber by activating SV4 for 5.3 - 7.0
seconds.
(7) 12.5L of SED-dye is added into SED reaction chamber by activating SV38 for 6.0 - 8.0 seconds
(8) Sample aspiration probe is driven backward to above rinse cup for 6.1 - 7.4 seconds by Y axis mechanism (STM4).
(9) Sheath chamber is replenished by activating SV35 for 11.9 - 16.9 seconds in maximum.
(10) Sample prove descends into rinse cup by driving Z axis motor (STM3) counter clockwise for 12.1 - 13.3 seconds.

UF-1000i S/M 2-20 June 2006

15.0 19.1 sec.

SRV returns to
initial position at
17.6 seconds.
Mixing
16.3-34.3 SV19
sec.
SV21

Mixing
16.3-34.3
sec.

UF-1000i S/M 2-21 June 2006

15.0 19.1 sec.
(11) Sample Aspiration probe at the sample aspirating position moves to above rinse cup driven by Z AXIS MOTOR (STM3) for 15.5 -16.9
seconds.
(12) 425L of BACT diluent and 62.5L of whole urine (487.5L in total) are dispensed into BACT reaction chamber by activating SV1 for
15.4 - 17.1 seconds. (Contents: BACT diluent: 425L, Whole Urine: 62.5L)
(13) SV11 is activated, and charging pump is driven as pre-drive for 16.0 - 16.3 seconds.
(14) Sample in SED chamber is charged into FCM measurement line by charging pump (STM8) operation while SV11 is activated for 16.4 - 18.6
seconds.
(15) Rinse cup is drained by activating PV46 for 16.4 - 23.4 seconds.
(16) Sheath liquid in rinse chamber is pushed into rinse cup for rinsing outside aspiration probe by activating SV7 for 16.4 - 19.3 seconds.
(17) 425L of BACT diluent and 62.5L of whole urine (487.5L in total) are dispensed into BACT reaction chamber by activating SV1 for
15.4 - 17.0 seconds.
(18) Sheath liquid in rinse chamber is pushed through whole urine pump, SRV, Aspiration probe and wasted into rinse cup for rinsing purpose
activating SV27 for 19.3 - 23.0 seconds.
(19) PV44 is activated to open SED sample charging path for 16.5 - 18.4 seconds.
(20) Sample charging pump descends for 16.6 - 18.1 seconds to charge SED sample from reaction chamber into FCM sample measurement line.
(21) SV30 is activated to form low speed sheath flow for 17.5 - 19.6 seconds.
(22) SV26 is activated for 17.5 - 18.1 seconds to remove air bubbles from sheath block.
(23) SV31 is activated for 18.7 - 19.8 seconds, and SV10 is activated for 18.7 - 20.0 seconds to rinse sample charge pump with rinse liquid
(UF-Sheath).

UF-1000i S/M 2-22 June 2006

19.1 28.4 sec.

UF-1000i S/M 2-23 June 2006

19.1 28.4 sec.
(24) SV14 is activated to open the FCM path for SED measurement for 19.1 - 28.2 seconds.
(25) Sheath Syringe ascends for 19.2 - 20.0 seconds as SED pre-drive.
(26) SV28 is activated to form fast Sheath Flow for 19.4 - 29.4 seconds.
(27) Sheath syringe ascending speed is changed for analysis mode, for 20.0 - 28.4 seconds.
(28) Rinse liquid is pushed into whole urine pump for by activating SV27 for 19.3 - 23.0 second, simultaneously, whole urine pump position is
initialized for 19.4 - 22.1 seconds.
(29) SV47 is activated for 22.7 - 23.8 seconds to blow remained liquid inside rinse cup off.
(30) Laser diode in optical unit emits for 19.2 - 28.4 seconds for SED measurement.
(31) SED mode count operation is performed for 22.3 - 27.8 seconds.
(32) SV25 is activated for 24.2 - 29.2 seconds to drain WC-1.
(33) SV37 is activated for 25.4 - 27.4 seconds to add 12.5L of BACT dye into BACT reaction chamber.

UF-1000i S/M 2-24 June 2006

28.4 - 28.9 sec.

UF-1000i S/M 2-25 June 2006

28.4 - 28.9 sec.
(34) Charging pump descends for 28.6 - 28.8 seconds while SV11 at sample charging path is activated for 28.5 - 28.9 seconds.
By this operation, sheath reagent is fed into FCM nozzle in reverse direction for rinsing purpose.

UF-1000i S/M 2-26 June 2006

28.9 31.5 sec.

UF-1000i S/M 2-27 June 2006

28.9 31.5 sec.
(35) PV44 is activated to open SED sample charging path for 29.1 - 29.6 seconds.
(36) SV22 is activated to drain SED reaction chamber for 29.1 - 30.0 seconds.
(37) SV10 is activated for 29.0 - 32.1 seconds, as the preparation of operation (38) and (39).
(38) SV11 is activated for 29.7 - 32.1 seconds while SV14 and SV23 are activated for 29.1 - 32.3 seconds to rinse inside of sample charging line
with sheath reagent. meanwhile, sheath syringe position is returned to its initial position for preparing BACT measurement.
(39) SV31 is activated for 29.0 - 29.6 seconds to rinse inside of charging pump with sheath reagent.
(40) SV 35 is activated for replenishing sheath chamber for 30.0 - 34.0 seconds.
(41) Sample aspiration probe is driven forward to its initial position for 30.5 - 31.7 seconds by Y axis drive mechanism.
(42) SV4 is activated for 31.2 - 32.9 seconds while SV18 is activated for 31.3 - 33.0 seconds to dispense 585L of SED diluent for rinsing
purpose.
(43) SED reaction chamber is drained by activating SV22 for 31.0 - 31.5 seconds.

UF-1000i S/M 2-28 June 2006

31.5 34.3 sec.

Mixing
16.3-34.3 SV19
sec.
SV21

Mixing
16.3-34.3
sec.

UF-1000i S/M 2-29 June 2006

31.5 34.3 sec.
(44) PV44 is activated for 31.8 - 32.3 seconds to rinse the SED sample charging path.
(45) SV32 is activated for 32.8 - 33.1 seconds to drain air filter.
(46) SV36 is activated for 32.8 - 38.8 seconds to drain WC2.
(47) To fill and familiarize sample measurement line with BACT diluent in prior to BACT sample charging, following operations are performed.
SV1 is activated for 32.5 - 34.2 seconds.
SV11 is activated for 33.1 - 34.3 seconds.
SV10 is activated for 35.4 - 35.7 seconds.
SV17 is activated for 32.4 - 34.3 seconds.

UF-1000i S/M 2-30 June 2006

34.3 35.1 sec.

SV19

SV21

UF-1000i S/M 2-31 June 2006

34.3 35.1 sec.
(48) To familiarize and to rinse inside FCM nozzle with BACT diluent in prior to BACT sample charging, following operations are performed.
SV16 is activated for 34.5 - 35.1 seconds to open FCM nozzle rinse line, meanwhile, charging pump ascends upward for 34.5 - 34.9 seconds,
and Low speed sheath flow is formed by activating SV30 for 34.5 - 35.1 seconds.

UF-1000i S/M 2-32 June 2006

35.1 38.0 sec.

UF-1000i S/M 2-33 June 2006

35.1 38.0 sec.
(49) PV45 is activated for 35.8 - 37.6 seconds to open BACT sample charging path.
(50) Charging pump descends for 35.9 - 37.4 seconds to charge BACT sample into BACT measurement line.
(51) Low speed sheath flow is formed by activating SV30 for 36.8 - 38.5 seconds,
(52) Air bubbles inside sheath block are removed by activating SV26 for 36.8 - 37.4 seconds.

UF-1000i S/M 2-34 June 2006

38.0 43.9 sec.

SV19

SV21

UF-1000i S/M 2-35 June 2006

38.0 43.9 sec.
(53) High speed sheath flow is formed by activating SV28 for 38.3 - 47.6 seconds.
(54) SV15 is activated for 38.0 - 43.8 seconds to open sample injection line between sheath syringe and flow cell.
Sheath syringe starts ascending for 38.1 - 43.8 seconds to inject charged sample into sheath flow cell.
(55) SV34 is activated for 36.1 - 44.1 seconds in maximum for replenishing rinse chamber with UF Sheath reagent.
(56) Laser diode in optical unit emits for 38.7 - 43.9 seconds for BACT measurement.
(57) BACT mode count operation is performed for 42.2 - 43.6 seconds.
(58) SV1 is activated for 38.3 - 40.0 seconds while SV20 is activated for 38.2 - 40.1 seconds to rinse BACT reaction chamber with 487.5L of
BACT diluent.
(59) BACT reaction chamber is drained by activating SV24 for 40.5 - 42.0 seconds.

UF-1000i S/M 2-36 June 2006

43.9 44.4 sec.

SV19

SV21

UF-1000i S/M 2-37 June 2006

43.9 44.4 sec.
(60) Charging pump descends for 44.0 - 44.4 seconds while SV11 is activated for 43.9 - 44.3 seconds.
By this operation, sheath reagent is fed into FCM nozzle in reverse direction for rinsing purpose.

UF-1000i S/M 2-38 June 2006

44.4 45.0 sec.

SV19

SV21

UF-1000i S/M 2-39 June 2006

44.4 45.0 sec.
(61) Inside sheath syringe, BACT sample charge path and inside BACT reaction chamber are rinsed by following operations.
SV15 and SV23 are activated for 44.5 - 48.4 seconds.
PV45 is activated for 44.5 - 45.0 seconds.
BACT reaction chamber is drained by activating SV24 for 44.5 - 45.4 seconds.
(62) SV31 is activated for 44.4 - 45.0 seconds while SV10 is activated for 44.4 - 47.1 seconds to rinse inside charging pump and sample charging
line.

UF-1000i S/M 2-40 June 2006

45.0 48.9 sec.

UF-1000i S/M 2-41 June 2006

45.0 48.9 sec.
(63) SV21 is activated for 45.4 - 46.0 seconds to rinse inside BACT reaction chamber

(64) BACT reaction chamber is drained by activating SV24 for 46.5 - 48.9 seconds, while PV45 is activated for 47.7 - 48.2 seconds to rinse BACT
sample charging path.

UF-1000i S/M 2-42 June 2006