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US005242820A
United States Patent [191 [11] Patent Number: 5,242,820
Lo [45] Date of Patent: Sep. 7, 1993

[54] PATHOGENIC MYCOPLASMA Lo et al (1989) Fatal Infection of Silvered Leaf Mon


keys . . .' Am. T Trop Med Hyg 40(4):399409.
[75] Inventor: Shyh-Ching Lo, Potomac, Md. Lo et a1 (1989) Virus-dike Infectious Agent . . . Am J
[73] Assignee: American Registry of Pathology, Trop Med Hyg 4l(5):586600.
Washington, DC. Marquart et a1 (Feb. 1985) Abstract Only Eur J Clin
Microbiol 4(1):73-74.
[21] Appl. No.: 710,361 Hu et a] (1990) Gene 93:67-72.
[22] Filed: Jun. 6, 1991 Primary ExaminerChristine M. Nucker
Assistant Examiner-D. R. Preston
Related [35. Application Data Attorney, Agent, or Finn-Venable, Baetjer, Howard &
Civiletti.
[63] Continuationin-part of Ser. No. 265,920, Nov. 2, 1988,
abandoned, which is a continuation-in-part of Ser. No. [57] ABSTRACT
875,535, Jun. 18, 1986, abandoned. The invention relates to a novel pathogenic myco
[51] Int. Cl.5 ....................... .. CIZN 5/00; C12N 5/02; plasma isolated from patients with Acquired Immune
C12N l/OO; C12Q 1/70 De?ciency Syndrome (AIDS) and its use in detecting
[52] US. Cl. ................................... .. 435/2401; 435/5; antibodies in sera of AIDS patients, patients with
435/872 AIDS-related complex (ARC) or patients dying of dis
[58] Field of Search ............... .. 435/870, 5, 872, 240.2 eases and symptoms resembling AIDS diseases. The
invention further relates to speci?c DNA sequences,
[56] References Cited antibodies against the pathogenic mycoplasma, and
PUBLICATIONS their use in detecting DNA or antigens of the patho
genic mycoplasma or other genetically and serologi
Marquart et a] (1985) Mycoplasrna-Like Structures . . . cally closely related mycoplasmas in infected tissue of
Eur J Clin Microbiol 4(l):73-74. patients with AIDS or ARC or patients dying of symp
Lo et al (1989) A Novel Virus-like Infectious Agent . . . toms resembling AIDS diseases. The invention still
Am J Trop Med Hyg 40(2):213226. - further relates to a variety of different forms of vaccine
Lo et al (1989) Identi?cation of M Incognitus . . . Am. J. against mycoplasma infection in humans and/or ani
Trop-Med. I-Iyg 4l(5):60l-6l6. mals.
Lo et al (1989) Association of the Virus-like Agent . . .
Am J Trop Med Hyg 4l(3):364-376. 2 Claims, 39 Drawing Sheets
US. Patent Sep. 7, 1993 Sheet 1 of 39 5,242,820

FIG. 1A FIG. 18
US. Patent Sep.7,1993 Sheet 2 of 39 5,242,820

FlG. 1C

FIG. 1D
US. Patent Sep. 7, 1993 Sheet 3 of 39 5,242,820

FIG. 2A FIG. 28
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US. Patent Sep. 7, 1993 Sheet 4 of 59 5,242,820

FIG.3A FIG.3B
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US. Patent Sep. 7, 1993 Sheet 5 of 39 5,242,820

FIG. 4A FIG. 48
US. Patent Sep.7,1993 Sheet 7 of 39 5,242,820

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U.S. Patent Sep. 7, 1993 Sheet 9 of 39 5,242,820
US. Patent Sep.7,1993 Sheet 10 of 39 5,242,820
US. Patent Sep. 7, 1993 Sheet 11 of 39 5,242,820

Am.9- g
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US. Patent Sep.7,1993 Sheet 12 of 39 5,242,820

FIG. 10A

FIG. 10B FIG. 100


US. Patent Sep.7,1993 Sheet 13 M39 5,242,820
US. Patent Sep. 7, 1993 Sheet 14 of .39 5,242,820

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US. Patent Sep.7,1993 Sheet 15 of 39 5,242,820

FIG; 13

FIG. 14
US. Patent sep. 7, 1993 sheet 16 0539 5,242,820

A FIG.- 15A FIG. 158


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US. Patent Sep. 7, 1993 Sheet 17 of-39 5,242,820

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US. Patent Sep. 7, 1993 Sheet 18 339 5,242,820

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US. Patent Sep. 7, 1993 Sheet 19 of 39 5,242,820

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