Professional Documents
Culture Documents
Disadvantages :
-High cost of nano drugs
-Nano particles may have different properties for an example
amalgamation of some particles may result in complete different
properties of that particle than all other individual particles.
-
a. Provide the full literature reference for the nanoparticle you chose. (1 pt)
Yu, T.; Malugin, A.; Hamidreza, G. Impact of Silica Nanoparticle Design on
Cellular Toxicity and Hemolytic Activity. Am. Chem. Soc. 2011, 5(7), 57175728.
e. Particle Characterization:
i. How was the particle characterized? (3 pts)
Particles are characterized by TEM imagining (For different shape and
sizes), Nitrogen adsorption and desorption isotherms for different shapes.
Dynamic Light scattering for size distribution profile and zeta potential
measurement for surface charge density.
By increasing, opaque light power density reduces the lights spot size, gives
narrower light beam.
c. Identify from primary literature at least one use of lithography in nanomedicine
i. Provide the full literature reference(s). (1 pts)
d. Assume you have just synthesized a new batch of PEGylated iron oxide
nanoparticles. Describe the tests you would use to characterize the particle
hydrodynamic radius, size of the polymer corona, size of the iron oxide core,
chemical composition/purity, and magnetic properties? (5 pts)
Hydrodynamic radius: Dynamic light scattering
Size of polymer corona: Infrared spectroscopy, EDS, XPS
Size of iron oxide cores: TEM
Chemical composition/ purity: Mass spectroscopy
Magnetic Properties: Zeta potential mesurement
5. Creation of Polymeric Materials:
a. Given the monomer with the structure of CH2=CHS (where S is a substituent)
i. Describe the steps involved in free radical polymerization of the
compound. Include the formula scheme(s) in each step. (4 pts)
c. Describe RAFTs and ATRP and how they differ from other types of
polymerization. Include a reaction scheme in each case. (4 pts)
Attached solution (Last page)
6. What are the advantages and disadvantages of in vitro vs in vivo testing for nanoparticle
toxicology (the creation of a table is recommended)? (6 pts)
7. Cellular uptake of Nanoparticles
a. Describe the various cellular uptake mechanisms and include schematic diagrams.
(5 pts)u
b. How does ____ influence particle uptake?
i. Size (2 pts)
ii. Geometry (2 pts)
iii. Porosity (2 pts)
iv. Surface chemistry (2 pts)
8. Influence of the protein corona on nanomaterials
a. What is the protein corona and why is it an important consideration for
nanomedicine both for in vitro and in vivo? (3 pts)
b. What factors influence the composition and thickness of the protein corona on a
nanoparticle? (2 pts)
9. Carmeda AB is a company that produces nanoscale coatings to improve the blood
compatibility of various medical devices. Look up information online about the
CARMEDA Bioactive Surface online and answer the following questions.
a. What is this coating made from and how is it synthesized? (2 pts)
b. How does this coating work to prevent/reduce thrombosis? (3 pts)
c. What specific blood components are targeted in its function? (2 pts)
d. Why might this strategy function effectively under only certain blood exposure
conditions? (3 pts)
e. Would you expect the effectiveness of this coating to differ between venous and
arterial flows? Please, justify your answer. (3 pts)