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A. Divisions of Microbiology
Microbiology can encompass many species of organisms. The divisions of microbiology
are...
1. Bacteriology is the science that studies bacteria. Numerous bacterial organisms are
found in our environment that are capable of causing disease in man.
a. Examples:
(1). Staphlococcus aureus can produce skin abscesses and food poisoning.
(2). Clostridium tetani may cause tetanus (also known as lock jaw).
2. Rickettsiology studies rickettsia. Rickettsia are sometimes smaller than bacteria and
exist as either spherical in form or they are shaped as rods. Rickettsia are responsible
transmitting typhus, Q-fever and rocky mountain spotted fever in man.
3. Virology is the scientific study of viruses.. Viruses are extremely small in size and are
composed primarily of nucleic acid. Common viral infections include small pox,
hepatitis and HIV
4. Protozoology scientifically studies protozoa. Protozoa are one-celled organisms that
transmit such diseases as amoebic dysentery and malaria.
5. Mycology is a branch of microbiology that studies fungi. Fungi may be unicellular or
multicellular and are often associated with such diseases as candidiasis and
aspergillosis.
(2). Heterotrophic organisms use organic matter for energy and the synthesis of
cell materials. Most bacteria are heterotrophic. Heterotrophic bacteria are
categorized according to the type of organic material upon which they feed.
(a). Strict (obligate) saprophytes are heterotrophic bacteria that obtain
nutritional need from non-living sources. Examples include:
(aa). Clostridium Tetani - (responsible for lock jaw) proliferates on
dead tissue.
(bb). The causative agent for the food poisoning known as
botulism.
(b). Strict (obligate) parasites are heterotophic bacteria that only grow on
other living organisms. Most pathogenic bacteria tend to be of this
type.
. Staph and strept infections are caused by this type of organism.
(c). Facultative bacteria are those heterotrophs that live and adapt in food
conditions that may vary. Sometimes they feed on dead tissue, at
other times they feed on living tissue.
6. Light Requirements - Green and purple pigmented bacteria use light as a source of
part or all of their energy. These organisms undergo a type of bacterial
photosynthesis resulting in the creation of food for the cell. When compared to other
bacterial forms, these species do appear in abundance. (In comparison, most
pathogens are killed by direct sunlight. Both ultraviolet rays and warmth harm
bacteria.)
7. Osmotic Pressure - Osmotic pressure is created by osmosis. Osmosis is the passage
of solvent (e.g., water) from a dilute to a more concentrated area through a
semipermeable membrane. When a cell is placed in a concentrated or hypertonic
environment, such as a salt water solution, water is drawn from the cell. As a result,
the cell may shrink and die. Bacterial cells vary in their resistance to unfavorable
osmotic conditions.
Many saprophytic microorganisms that cause food spoilage are dehydrated by
immersion in strong brines or syrups. They may not always die, but at least they do
not grow and spoil the food product. Shelf-life of such products as pickles, corned
beef, salt fish, jams, and jellies is lengthened by this principle.
A. Levels of Control: Three terms are often used to describe the control of
microbial populations.
1. Disinfection may be described as any process that destroys, neutralizes, or
inhibits pathogenic organisms. (“a process that destroys infectious agents found
on inanimate objects.”) Often, vegetative organisms are destroyed, but not
spores.
A definition of disinfection may also suggest that not all microorganisms are
destroyed. An example would be the pasteurization of milk whereby the harmful
pathogens are killed, but harmless organisms are not. Disinfection is not always
selective. Some harmless organisms may die in the process.
2. Sterilization is defined as any process that completely removes or destroys all
living organisms in or on an object. Sterilization also destroys spores. Think of
sterilization as a process that has a 100% “kill rate.” In contrast, disinfection
results in a “kill rate” less than 100 %.
3. Antisepsis is a process that prevents and/or combats infection or growth of
pathogenic bacteria on living tissue. Antisepsis occurs when an antiseptic product
is applied on a skin surface.
B. Types of Infection
1. Exogenous vs. Endogenous Infection
a. Exogenous infection originates outside of the host.. For example, the common
cold is passed from one person to another. The HIV virus is considered
exogenous , as well.
b. Endogenous infection originates within a cell or organism. Opportunistic
organisms that normally live within the host may proliferate when conditions are
favorable. For example, injury to the host may occur when indigenous flora,
such as staph or strept, grow out of control.
2. Acute vs. Chronic Infection
a. Acute infection will last for a short period of time (if properly treated).
Sore throats, common colds, and flu infections are considered acute infections.
b. Chronic infection will last for a long period of time. Some may result in death
of the host. Hepatitis, tuberculosis, and HIV may be considered chronic
infections.
F. Portals of Entry
Portals of entry are pathways by which microorganisms normally enter the host’s body.
The particular route depends upon the microorganism and, to an extent, the type of
vector involved. Sometimes pathogens cause infection only if they enter their obligate
portal of entry. For instance, the dysentery bacillus would cause no harm of rubbed into
a skin lesion. However, this same organism can produced fatal results of ingested.
Examples of said portals include:
1. Skin wounds
2. Mucous membranes
3. Respiratory tract,
4. Digestive tract,
5. Genito-urinary tract,
6. Placenta.
From the various portals of entry, organisms may pass into the circulatory system and
initiate a secondary infection in some internal organs or in membranes.
G. Portals of Exit - Microorganisms leave the body via a “portal of exit” as they
continue their journey from one host to the next. In humans, microorganisms may enter
one portal, but will leave through another. Example: The portal of entry for the hepatitis
virus can be through a contaminated hypodermic needle, whereas the portal of exit
would be the direct contact with contaminated blood.
To control occupational exposure of pathogens, embalmers should be aware of the
portals of exit. Examples include:
1. Skin wounds
2. Mucous membranes
3. Respiratory tract,
4. Digestive tract,
5. Genito-urinary tract,
6. Placenta.
VI. IMMUNOLOGY
Immunology is the science that studies antigen-antibody reactions. Immunity is a
relative term describing one's ability to resist or overcome a harmful antigen, such as an
infecting bacterium or virus. The term may also refer to any state in which the individual
has produced an immunoglobulin to a specific antigen. The goal of immunity is to
provide resistance to harmful microorganisms.
A. Antigens are substances that stimulate the formation of antibodies within the host.
They react with the antibody in a demonstrable way. Most often, antibodies are large
protein molecules. Exotoxins and extracellular enzymes (originating from microbes)
antigen examples. Antigens may enter the body through infective routes, such as in
food or by way of droplet infection. Substances normally native to the body do not
ordinarily produce the immune response.
C. Types of Immunity
Many infectious diseases produce a specific immunity that does not protect against
other infections. Some diseases confer no immunity at all. Others produce some
immunity, while another group induces a possible life-long immunity.
Categorically, there are two major types of immunity. The difference depends upon
the source of the antibody and the degree of protection and duration.
1. Natural (innate) Immunity - Natural immunity is based on genetics or heredity.
It is not based on a theory of an individual’s prior contact with an infectious
organism.
A species, race, or individual may exhibit natural immunity. For example, man
does not develop hog cholera or distemper, whereas an animal would.
Additionally, some races within a species may be more susceptible than others to
a disease. For instance, the wild mouse may resist pneumococcal infection,
whereby the white mouse is susceptible
4. Neisseria Gonorrhea - a gram negative diplococcus that may be killed with penicillin
therapy. Pili is present on the organism’s surface. Pili allows the organism to adhere
to other surfaces. Hence, the organism readily receives nutrients from the surrounding
environment. Pili serve to enhance the organism’s virulence.
a. This organism is responsible for causing the following diseases:
(1). Gonorrhea - Gonorrhea is a common venereal disease.
(2). Ophthalmia Neonatorum - Ophthalmia neonatorum is characterized by an
inflammation of the eye in newborn infants (contracted during childbirth by
gonorrheal infection from the mother and may result in blindness if left
untreated).
b. Portal of entry and exit - Genito-urinary tract of the infected person.
c. Mode of transmission - Gonorrhea is transmitted by direct contact through
unprotected sexual intercourse.
d. Factor influencing virulence - Presence of Pili.
5. Neisseria Meningitidis (a.k.a. meningococcus)
a. Neisseria Meningitidis is responsible for the following diseases:
(1). Epidemic Meningitis - Epidemic meningitis causes a very dangerous and often
fatal inflammation of the meninges (coverings of the brain and spinal cord).
Also known as meningococcus meningitis, this disease may attack many
people at one time. Symptoms include; sudden fever, intense headache and
nausea. Delirium and coma may follow. The patient could die within six
hours after the initial onset.
(2). Meningococcemia - Meningococcemia may spur thrombosis formation in
many small blood vessels of organs. Meningitis could be a complication
stemming from meningococcemia. One might observe petechial hemorrhages
on the skin of the infected patient.
b. Portals of entry and exit - The respiratory tract (nasopharynx), skin, and mucous
membranes.
c. Modes of transmission - The primary mode of transmission is by way of contact
with aerosol droplets, specifically an infected person coughing or sneezing on
another. Other modes include; physical contact with contaminated blood, spinal
fluid, saliva, and sputum (“hocker,” “lugie”).
(3). Tissue Gas - Although this is not a disease, tissue gas is of concern to the
embalmer since this is postmortem gas formation that can spread throughout
the body. Unclean instruments (esp. trocar) may carry this organism from one
body to another. Instruments used in the preparation of know tissue gas cases
must be sterilized before they are used again.
9. Salmonella Typhi -
a. Salmonella Typhi is responsible for the following disease:
(1). Typhoid Fever - Typhoid fever is an acute infectious disease caused by
salmonella typhi and is characterized by a gradual rise in fever and systematic
invasion throughout the lymphatic system and bloodstream. The end result
yields a condition known as bacteremia.
b. Portals of entry and exit - The digestive tract.
c. Mode of transmission - Typhoid fever is spread via fecal contamination of water
and food. In sum, careless food handlers and contaminated water supplies serve as
vehicles of transmission.
10. Francisella Tularensis - A gram-negative, non-motile bacillus responsible tularemia,
or "rabbit fever."
a. Francisella Tularensis is responsible for the following disease:
(1). Tularemia - Tularemia is a disease marked by chills, fever, and swollen lymph
nodes in the area of infection. Additionally, tularemia can be incapacitating
for long periods of time.
b. Portals of entry and exit - The gastro-intestinal tract and the respiratory tract.
c. Modes of transmission - People working with small animals may encounter
francisella tularensis by inhaling dust found in hay contaminated with infected
feces. Cleaning a contaminated rabbit cage would be a likely activity that could
result in infection. Ticks, fleas, deer flies, and other arthropods found on rodents,
rabbits, and other mammals transmit tularemia from one animal to another and
eventually to humans. Another mode of transmission involves contaminated water
sources. Infected animals will occasionally die on a stream’s bank or near a water
source used for human consumption.
15. Others
Organism Disease
a. Vibrio Cholerae Asiatic Cholera
b. Yersinia Pestis Plague
c. Klebsiella Pneumoniae Pneumonia and urinary infection
d. Clostridium Botulinum Botulism
e. Bacillus Anthracis Anthrax
f. Hemophilus Influenzae Influenzal Meningitis
g. Proteus sp. Secondary infection of burns
h. Shigella sp. Bacillary Dysentery
i. Pseudomonas sp. Secondary infection of burns
j. Leptospira Interrogans Leptospirosis
k. Escherichia Coli Enteritis
l. Campylobacter jejuni Campylobacteriosis
B. MYCOPLASMAS
Mycoplasmas are a genus of bacteria lacking cell wall material due to the cell's genetic
inability for its synthesis. Some would argue that these are the smallest free living
organisms presently known. They range in size between viruses and bacteria.
C. RICKETTSIA
Rickettsia are a genus of gram-negative, pathogenic intracellular parasitic bacteria shaped
as either spheres or rods. They range in size between viruses and bacteria.
Morphologically, they resemble the bacteria. Biologically (because of their obligate
parasitism), they are related to viruses. Rickettsia are widespread in nature and are found
in arthropods (a species of insects and ticks) and mammals. Except for the louse,
rickettsia do not harm arthropods or mammals. Mammals become infected with rickettsia
through the bites of infected ticks, mites, lice, or fleas. Man is an accidental host. Once
transmitted to man, the rickettsia quickly spread by blood to other body areas and tend to
localize in endothelial cells of small blood vessels. The symptoms of man’s disease
include fever, rash, stupor, and terminal shock. Rickettsial diseases can be highly fatal.
There are at least four diseases caused by rickettsial infection.
D. CHLAMYDIA
Chlamydia are a large group of non-motile, gram-negative intracellular parasites.
Closely related to gram-negative bacteria, chlamydia were once thought to be large
viruses. They contain cell walls with high lipid content. Chlamydias appear to
parasitize their host cells to gain energy since they lack energy yielding enzyme
systems.
Chlamydias are responsible for eye infections that could result in blindness from
corneal infection and conjunctivitis in newborns as a result of contact with an infected
mother's birth canal during expulsion. Chlamydias are also responsible for urethritis in
adults.
5. Immunological
a. HIV is responsible for acquired immune deficiency syndrome.
F. FUNGI
Fungi are more complex and higher in evolutionary development than bacteria. They are
eucaryotic, meaning they are plants with a true nuclei. Molds, yeasts, mildews, rusts,
smuts, and puffballs are common types of fungi. Fungi characteristically enter the body
through the respiratory tract and invade the lungs or other internal parts of the body
causing disease. These diseases are sometimes referred to as deep of systemic mycoses.
Other fungal disease are considered "contact diseases" due to the fact that the
susceptible individual will directly come in contact with fungi on the skin surface
G. PROTOZOA
Protozoa are microscopic, single-celled animals.
H. PRION
A small proteinaceous infectious particle that is resistant to most procedures that modify
nucleic acids.