GENETICA

LABORATORIO VIRTUAL SOBRE MUTACION

1. Revisen la pagina
http://virtuallaboratory.colorado.edu/BioFun-
Support/labs/OnMutation/OnMutation.html

2. En el menu del lado izquierdo entren a cada uno de los temas (hay
7 temas)

EN EL LABORATORIO CORREREMOS LOS EXPERIMENTOS,

TOMAN DATOS Y RESPONDERAN LAS PREGUNTAS DE CADA
SECCION.

ACA LES DAMOS LOS FORMATOS IMPRESOS PARA RESOLVER

LAS PREGUNTAS

ASEGUREN QUE POR LO MENOS TIENEN PORTATIL POR GRUPO

(NO CORRE EN iPAD)

Informacion de apoyo

Diluciones seriadas =
https://www.youtube.com/watch?v=pmRUBYlPMBM

Clculo de PFU = https://www.youtube.com/watch?v=P14-ep2kag8

 LABORATORIO VIRTUAL SOBRE MUTACION

Nombres:____________________________

Lab report part 1

1. Note your estimated of the original phage stock titer (and show your
data).

2. Repeat the clearing experiment two times to check whether your

estimate is reproducible.

3. Use the vSpec to get an estimates of time to lysis (show your

data)

Questions to answer: What factors contribute to error in your estimate

of viral life cycle time?

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Lab report part 2

Questions to answer

Why did the phage disappear from the supernatant and where did
they go?

When did phage reappear in the supernatant?

 When did PFU appear in the pellet fraction and how did this time
relate to the appearance of phage in the supernatant?

Make a model of phage infection, assembly and release that fits

your data.

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Lab report part 3

1. Outline the steps in the burst size experiment and explain why it
was constructed the way it was.

2. On the plates with plaques, what was the average burst size?

Question to answer: In the first part of experiment its critical to dilute the
phage + bacteria mixture quickly. After diluting there is no particular
urgency in plating out the samples. Explain why this is the case.
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Lab report part 4

Based on your data, is it likely that MOI is the cause of the

differences in burst size?

Lab report part 5

Note down your results.

Was your TR cultures resistant to T4 upon retest?

Why did you add T4 phage to wild type E. coli? What did that
control for?

Why did you test your resistant strains using T1 phage? What did
that tell you?

Based on your previous observations, will bacteria that fail to bind

T4 phage also fail to bind T1 phage?
 What would be the molecular reasoning behind your conclusion?

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Lab report 6

Record the variance and the mean of your fluctuation analysis

(above); is it high or low.

Answer these questions:

Why would induced mutation produce low levels of variation

between cultures?

Do the bacteria somehow "decide" to produce mutations that lead

to phage resistance?