EEI RESULTS TABLE

Egg white + Sulphates

Whats being tested Time taken to loose Peaks

Control # 1 (Egg white only) 4.11
Control # 2 (Egg white only) 5.04
Control # 3 (Egg white only) 4.28
CuSO4 # 1 (Egg white+ Copper sulphate) 12.23
CuSO4 # 2 (Egg white+ Copper sulphate) 12.33
CuSO4 # 3 (Egg white+ Copper sulphate) 12.14
FeSO4 # 1 (Egg white+ Iron sulphate) 11.11
FeSO4 # 2 (Egg white+ Iron sulphate) 11.01
FeSO4 # 3 (Egg white+ Iron sulphate) 11.31
ZnSO4 # 1 (Egg white+ Zinc sulphate) 11.40
ZnSO4 # 2 (Egg white+ Zinc sulphate) 12.12
ZnSO4 # 3 (Egg white+ Zinc sulphate) 11.40

Colorimeter Controls
Blue Green Red Orange
Dist. H2O 0.000
EGG 1.940 2.122
CuSO4 0.1M 0.399
FeSO4 0.1M 0.161
ZnSO4 0.1M 0.000

pH Controls & Result Averages

0.1M 0.05M 0.02M 0.002M 0.0009M
CuSO4 3.3 3.6 3.9 4.4 4.8
FeSO4 2.6 2.7 2.9 3.5 3.9
ZnSO4 4.6 4.9 5.0 6.6 5.5
Egg White 9.0
CuSO4 + Egg White 5.6 6.0 6.4 8.4 8.3
FeSO4 + Egg White 2.9 3.9 4.7 7.6 8.1
ZnSO4 + Egg White 6.6 6.8 6.9 8.1 8.7

All tested positive for protein using biuret test

 Dilutions

Diluting Molar to Molar solutions

ml of stock solution required= Required Molarity x Volumed required (in ml)

Stock Molarity

From 0.1M stock solutions of CuSO4, FeSO4 & ZnSO4 make the following dilutions 0.05M. 0.02M, 0.002M & 0.0009M.

0.1M > 0.05M

30ml of stock solution required= Required Molarity (0.05) x Volumed required (60 ml)

Stock Molarity (0.1)

0.1M > 0.02M

12ml of stock solution required= Required Molarity (0.02) x Volumed required (60 ml)

Stock Molarity (0.1)

0.05M > 0.002M

2.4ml of stock solution required= Required Molarity (0.002) x Volumed required (60 ml)

Stock Molarity (0.05)

0.002M > 0.0009M

27ml of stock solution required= Required Molarity (0.0009) x Volumed required (60 ml)

Stock Molarity (0.002)

 pH Results
Test Results
#1 #2 #3
CuSO4 0.1M + 5.7 CuSO4 0.1M + 5.6 CuSO4 0.1M + 5.6
Egg White Egg White Egg White
FeSO4 0.1M+ Egg 2.9 FeSO4 0.1M+ Egg 2.9 FeSO4 0.1M+ Egg 2.9
White White White
ZnSO4 0.1M+ 6.5 ZnSO4 0.1M+ 6.7 ZnSO4 0.1M+ 6.8
Egg White Egg White Egg White

#1 #2 #3
CuSO4 0.05M + 6.0 CuSO4 0.05M + 6.1 CuSO4 0.05M + 5.9
Egg White Egg White Egg White
FeSO4 0.05M+ 3.9 FeSO4 0.05M+ 3.9 FeSO4 0.05M+ 3.8
Egg White Egg White Egg White
ZnSO4 0.05M+ 6.8 ZnSO4 0.05M+ 6.7 ZnSO4 0.05M+ 6.8
Egg White Egg White Egg White

#1 #2 #3
CuSO4 0.02M + 6.6 CuSO4 0.02M + 6.4 CuSO4 0.02M + 6.4
Egg White Egg White Egg White
FeSO4 0.02M+ 4.5 FeSO4 0.02M+ 4.8 FeSO4 0.02M+ 4.8
Egg White Egg White Egg White
ZnSO4 0.02M+ 6.8 ZnSO4 0.02M+ 6.9 ZnSO4 0.02M+ 7.0
Egg White Egg White Egg White

#1 #2 #3
CuSO4 0.002M + 8.4 CuSO4 0.002M + 8.6 CuSO4 0.002M + 8.2
Egg White Egg White Egg White
FeSO4 0.002M+ 7.6 FeSO4 0.002M+ 7.7 FeSO4 0.002M+ 7.6
Egg White Egg White Egg White
ZnSO4 0.002M+ 7.9 ZnSO4 0.002M+ 8.2 ZnSO4 0.002M+ 8.3
Egg White Egg White Egg White

#1 #2 #3
CuSO4 0.0009M 8.2 CuSO4 0.0009M 8.3 CuSO4 0.0009M 8.4
+ Egg White + Egg White + Egg White
FeSO4 0.0009M+ 8.1 FeSO4 0.0009M+ 8.2 FeSO4 0.0009M+ 8.1
Egg White Egg White Egg White
ZnSO4 0.0009M+ 8.7 ZnSO4 0.0009M+ 8.8 ZnSO4 0.0009M+ 8.7
Egg White Egg White Egg White

1. Put 10cm of solution into test tube, write the conc. on the test tube.

2. Place a sample into the colorimeter and record the result.

3. Add 2cm of egg white to the test tube, mix for 3 minutes, Rest for 6minutes, Mix for 1 minute.

4. Place a sample into the colorimeter and record the result.

 Rates of Absorption & Transmission

CuSO4 Conc. Abs Before Abs After Difference Trans % before Trans % After % Difference
0.1M 0.399 3.000 2.601 40.1% 0.0% 40.1%
0.1M 0.399 3.000 2.601 40.1% 0.0% 40.1%
0.1M 0.399 3.000 2.601 40.15 0.0% 40.15
0.05M 0.177 3.000 2.823 66.6% 0.0% 66.6%
0.05M 0.177 3.000 2.823 66.6% 0.0% 66.6%
0.05M 0.177 3.000 2.823 66.6% 0.0% 66.6%
0.02M 0.086 3.000 2.914 82.0% 0.0% 82.0%
0.02M 0.086 3.000 2.914 82.0% 0.0% 82.0%
0.02M 0.086 3.000 2.914 82.0% 0.0% 82.0%

0.002M 0.011 0.653 0.642 100% 22.1% 77.9%

0.002M 0.011 0.671 0.660 100% 21.7% 78.3%
0.002M 0.011 0.834 0.823 100% 14.8% 85.2%
0.0009M 0.008 0.397 0.389 100% 40.6% 59.4%
0.0009M 0.008 0.399 0.391 100% 41.7% 58.3%
0.0009M 0.008 0.385 0.377 100% 41.5% 58.5%

FeSO4 Conc. Abs Before Abs After Difference Trans % before Trans %After % Difference
0.1M 0.161 3.000 2.839 69.2% 0.0% 69.2%
0.1M 0.161 3.000 2.839 69.2% 0.0% 69.2%
0.1M 0.161 3.000 2.839 69.2% 0.0% 69.2%
0.05M 0.079 3.000 2.921 83.5% 0.0% 83.5%
0.05M 0.079 3.000 2.921 83.5% 0.0% 83.5%
0.05M 0.079 3.000 2.921 83.5% 0.0% 83.5%
0.02M 0.057 3.000 2.943 87.9% 0.0% 87.9%
0.02M 0.057 3.000 2.943 87.9% 0.0% 87.9%
0.02M 0.057 3.000 2.943 87.9% 0.0% 87.9%

0.002M 0.016 0.958 0.942 96.5% 10.9% 85.6%

0.002M 0.016 0.939 0.923 96.5% 11.4% 85.1%
0.002M 0.016 0.962 0.946 96.5% 10.8% 85.7%
0.0009M 0.013 0.608 0.595 98.3% 24.6% 73.7%
0.0009M 0.013 0.677 0.664 98.3% 20.9% 77.4%
0.0009M 0.013 0.673 0.660 98.3% 21.1% 77.2%

ZnSO4 Conc. Abs Before Abs After Difference Trans % before Trans %After % Difference
0.1M 0.000 3.000 3.000 100% 0.0% 100%
0.1M 0.000 3.000 3.000 100% 0.0% 100%
0.1M 0.000 3.000 3.000 100% 0.0% 100%
0.05M 0.000 3.000 3.000 100% 0.0% 100%
0.05M 0.000 3.000 3.000 100% 0.0% 100%
0.05M 0.000 3.000 3.000 100% 0.0% 100%
0.02M 0.000 3.000 3.000 100% 0.0% 100%
0.02M 0.000 3.000 3.000 100% 0.0% 100%
0.02M 0.000 3.000 3.000 100% 0.0% 100%

0.002M 0.000 1.265 1.265 100% 7.9% 92.1%

0.002M 0.000 1.250 1.250 100% 8.2% 91.8%
0.002M 0.000 1.136 1.136 100% 8.3% 91.7%
0.0009M 0.000 0.510 0.510 100% 31.7% 68.3%
0.0009M 0.000 0.491 0.491 100% 32.7% 67.3%
0.0009M 0.000 0.436 0.436 100% 36.4% 63.6%