Professional Documents
Culture Documents
Heraeus Megafuge 8
Sorvall ST8
Thermo Scientific SL8
PN: 12007200-01
The original version of this manual is valid. Thermo Scientific cannot guarantee the accuracy or completeness of subsequent copies
TABLE OF CONTENTS
Introduction iii
Revision Control iv
INTRODUCTION
PURPOSE
REVISION CONTROL
Heraeus Megafuge 8
Sorvall ST8
Thermo Scientific SL8
Allowable
Rotor /
Rotor name imbalance max. speed max. x g max radius
bucket
[g]
75005701 TX-150
150 Rotor 10 4500 3260 14.4
75005702 TX-150
150 Round Bucket 10 4500 3260 14.4
75005703 TX-150 Ovall bucket 10 4500 3260 14.4
75005704 TX-100S 10 4500 3215 14.2
75005705 TX-100 10 4500 3260 14.4
75005706 M-10
10 Rotor 10 4400 2576 11.9
75005723 M-10
10 Unsealed Bucket 10 4400 2576 11.9
75005721 M-10 Sealed Bucket 10 4400 2381 11.0
75005600 MT-12 Rotor 4 13000 16438 8.7
75005709 HIGHConic III Rotor 10 8700 10155 12.0
75003623 CLINIConic Rotor 10 4400 3030 14.0
75005719 MicroClick 30x2 Rotor 4 14000 21694 9.9
75005715 MicroClick 24x2 Rotor 4 16000 24327 8.5
75003473 Hematocrit Rotor 1 capillary 13300 16810 8.5
75005643 PCR Strip Rotor 8x8 4 15000 17860 7.1
SERVICE 2-1
SERVICE
Servicing schedule
This chapter describes the maintenance work to be performed on a yearly basis.
A checklist can be found in the attachment A-1.
Temperature level
Check the air inlet slots for free ventilation. Insufficient air flow will lead to an inadmissible
temperature rise of the motor and electronic parts.
Imbalance behavior
install available and empty rotors and check the imbalance behavior with rotor dependant cut off
and run through weights (see page 2-18) and replace worn anti vibration mounts, faulty main
board or sensor cover.
Braking circuit
Check the function of the braking circuit (warming up of brake resistor, even and noiseless brake
effect) and replace defective parts in case of malfunction.
Trouble shooting
Malfunctions without error code
Possible Error
Error Error Cause Corrective Procedure
Source
power switch with
integrated thermal
over current cut out
Check supply voltage at X16. If no voltage
no mains mains fuse or circuit is present, check power switch, instrument
voltage supply breaker failed socket and voltage supply.
Replace defective parts
Display faulty mains cord or
remains off instrument socket
Display
backlight is
lit, but
Check for interferences such as radios,
nothing
micro wave ovens, etc. Check for good
displayed data faulty connection
grounding connection. Check the display
or connection from main board to
cable (SSTP shielded cable). If no other
display disturbed display
error source can be identified, replace main
shows
board.
cryptically
(wrong)
characters
LCD
Press any button. To switch off the LCD
powersafe
powersafe mode enter the system menu.
No display mode
backlight
faulty
faulty backlight Replace display.
backlight
Possible Error
Error Error Cause Corrective Procedure
Source
Check anti vibration mounts and replace if
worn anti vibration
worn or older than 3 years.
mounts
(see page 5-6)
Trunnions of
Inform users that trunnions must be
swinging bucket
cleaned and greased on a regular basis.
rotor not greased
See user manual for more information.
mechanical sufficiently
Drive
makes Buckets of swinging
noises, bucket rotor not Check weight index on buckets.
no good weight identical
separation
result Turn motor by hand. If a scratching noise is
bad motor bearings
audible, replace complete motor.
Error-Codes
Should multiple errors occur at the same time, the centrifuge will display up to 3 errors at once, by
alternating them on the display.
Error Description
E-001 System clock unstable
E-002 Main state machine corrupted (software)
E-005 System reset by watchdog
E-008 Check sum error ROM test
E-010 NV-RAM communication error
E-011 NV-RAM does not match E-PROM
E-012 Check sum error NV-RAM constant area
E-014 Chamber over temperature: t > 50C
E-015 Temperature sensor open
E-016 Temperature sensor short
E-017 Speed above rotor recognition speed limit
E-019 Rotor code not in rotor table
E-020 Rotor code corrupted
E-021 Rotor recognition and speed 1 measurement disturbed
E-022 Speed 2 measurement disturbed
E-023 Difference between speed 1 and speed 2 signals
E-025 Lid could not be opened
E-027 State of lid latch micro switches implausible
E-029 Motor does not turn
E-030 Control voltage failure
E-031 Motor over temperature: t > 150C
E-033 High pressure in cooling system
E-034 Over voltage in power circuit
E-036 Over voltage or short circuit at inverter
E-038 Lid latch current abnormal (Speed 0)
E-040 Acceleration too slow
E-041 Offset voltage of imbalance sensor implausible
E-045 Safety test during restart failed
E-046 Lid opened during run
E-048 Software state machine error
E-050 Software state machine error
E-051 Software state machine error
E-052 Software state machine error
E-053 Software primary mask reading error
E-054 Software primary mask writing error
E-060 Chamber under temperature: t < -20C
E-072 Software state machine error
SERVICE 2-7
Error Description
E-074 Latch motor control fault
E-077 System health check error
E-078 Set speed above max speed
E-079 Speed above rotor maximum: >25rpm
E-080 Rotor detection failed
E-081 Second top speed check failed
E-083 Brake resistor over temperature
E-098 Imbalance detected
E-101 Temperature measurement shows great deviations to expected values
E-104 Temperature measurement could not be completed
E-105 Software state machine error
E-106 Lid latch current abnormal (Speed >0)
E-107 Main board over-temperature
SERVICE 2-8
.
SERVICE 2-13
Test Points
Lid latch stepper motor Pin 1-2 46 Ambient at 20C, plug disconnected from
X8 Pin 3-4 46 main board
SERVICE 2-14
15 V control circuit
power supply lit All cables connected, power switch ON
LED V79 (red)
The system menu allows the user to make advanced settings and read units values as additional
information for service calls.
The service menu provides guidance for troubleshooting, such as error history list, statistics
and tests.
SYSTEM MENU
DOWN to select
YES or NO
SYSTEM MENU
SERVICE 2-17
Number of
Total number Last 4 digits runs with this
of runs of rotor code type of rotor
Use SPEED UP
Hours Minutes
and DOWN to
toggle between
screens
Unit hours XXXXXhXX
SERVICE MENU
View device stats? Drive hours XXXXXhXX
SERVICE MENU SERVICE MENU
Lid lock cycles XXXX
Use SPEED UP SERVICE MENU
and DOWN to
toggle between Total counter of lid Use TIME UP
screens open / close cycles and DOWN to
toggle between
screens
Press START to start the test.
Then press the buttons as they are
Start button test? requested in the display. In case of
SERVICE MENU malfunction (no entry is recognized
by the micro controller), the next
button will be requested to press
after 30 seconds.
Use SPEED UP
and DOWN to 1. No display
toggle between element is lit
screens
Imbalance Behavior
Install a rotor from the table below in unloaded condition (without adapter or other inserts)
Insert rotor dependent cut-off weight in all buckets or Sample holders one after the other. The
centrifuge must show an Imbalance message and stop.
Insert the run through weight in the same manner, the centrifuge must run through 4 times to
maximum rotor speed without giving an alarm.
Always use a weight which can be placed at the bottom of the bucket or sample holders (e.g.
screw nuts and flat washers with cable tie)
Electronic components
Clean dusty components carefully with a dry and soft brush and remove loose dust with a
vacuum cleaner.
Fans (refrigerated units only)
Scratch off carefully with a knife or similar tool the crusted dirt from the fans blades. Resulting
grooves or marks must be removed subsequently with a fine abrasive cloth. Loose dirt is to
remove with an absorbent cloth or vacuum cleaner.
Vent holes
Remove dust and dirt from the vent holes of the unit und clean the condenser (refrigerated
units only) using a brush and vacuum cleaner.
SERVICE 2-19
Spezifaktionen fr Megert
10k - TRMS, DC - 5kHz oder mehr
1500 0,22F 5%
- Eingangswiderstand > 1M
5% 5%
- Toleranz 5% oder besser
- Crest Faktor 5 oder besser
0,022F V
5% AC
Specifaction for the meter
500 - TRMS, DC - 5kHz or more
1% - Input resistance > 1M
- Tolerance 5% or better
- Crest Factor 5 or better
L (N)
Krperstrom:
N (L) accessible current: I [mA] = U [mV] / 500:
PE Umax = 250mV Imax = 0,5mA
FUNCTIONAL DESCRIPTION 3-1
FUNCTIONAL DESCRIPTION
Display 3-5
Block functions
The Megafuge 8 / ST8 / SL8 is a non-refrigerated laboratory tabletop centrifuge with microprocessor
control, noise reduced induction drive motor and automatic rotor, speed and imbalance detection.
3~ MOTOR
REAR PANEL
LID LOCK
OVER TEMPERATURE
BRAKE RESISTOR
MAIN BOARD
KEYPAD
FUNCTIONAL DESCRIPTION 3-3
Noise filter and power pack for DC intermediate circuit with line voltage configuration (1)
Power pack for low voltage supply of microprocessor part (2)
Frequency inverter equipped with automatic loading (3) and voltage (brake) controlling
circuits (4)
Microprocessor part with CPU and NV RAM (5)
Lid lock control circuit with driving component for lid latch stepper motor (6)
Speed, imbalance and rotor detection circuit (7)
Display and keypad control circuit (8)
Buzzer circuit (9)
Exchangeable NV-Ram containing specific data of the unit (NV)
Additional circuits for refrigerated models.
4 2
3 6
7
1
NV
9
5
8
FUNCTIONAL DESCRIPTION 3-4
Power Pack
The power pack consists of 1 transformer, 2 rectifiers (single diode) and 2 voltage regulators. It
generates the following low voltage levels:
Intermediate Circuit
The DC intermediate circuit serves as an energy store between the AC power input and the
transmitted motor performance. The intermediate circuit consists of a line voltage selection switch, a
heat-sink-cooled bridge rectifier and two reservoir capacitors which are charged whenever the
centrifuge lid is closed (K1 activated). The presence of the DC voltage is indicated by LED V41.
When switching the unit off, the DC voltage of the intermediate circuit is discharged across a path of
resistors.
Brake Path
Electrical power is fed back into the intermediate circuit during motor deceleration (motor acts as
generator). This braking power is transformed into heat by the connected resistor so that the
intermediate circuit voltage does not rise to an excessive level. The brake resistor is switched into the
intermediate circuit by a fast switching transistor (pulse-width modulation, synchronised by the double
mains frequency). This transistor is voltage dependent controlled by a self-acting stage (closed loop).
Frequency inverter
The 3-phased motor is provided with chopped direct voltage blocks (chopped frequency approx.
5kHz, amplitude is height of DC intermediate voltage). These blocks are variable in frequency and
pulse-width modulation, 120 de-phased. These 3 phases are controlled during acceleration, running
at set speed and deceleration (e.g. for small speed: low frequency and small pulse-width length will
be affected). The frequency inverter is protected against over current, over temperature and over-
voltage.
The controller block includes the central processor unit (CPU) LPC1764 programmed with software to
control the centrifuge and a data storage (NV-RAM). Current versions and revisions of software and
data can be seen in the system menu.
Software is loaded into the LPC1764 via a serial interface on the main board X6 and cannot be
upgraded in the field. The NV-RAM data can be updated by replacing the entire NV-RAM with one of
the latest revision.
NV-RAM data includes rotor parameters, such as max speed and g-force, acceleration and
deceleration profiles and imbalance behaviour.
FUNCTIONAL DESCRIPTION 3-5
Display
The LCD is attached to the front panel. The connection to the main board is done by a RJ45 cable.
Data transfer as well the low voltage supply of 5V is verified. The operating keys are managed by an
own connection.
Rotor detection
Speed detection
Imbalance detection 4
Vibration
ELECTRICAL DIAGRAMS
Overview 4-2
Controller 4-7
3~ MOTOR
REAR PANEL
LID LOCK
OVER TEMPERATURE
BRAKE RESISTOR
BRAKE RESISTOR
DISPLAY
MAIN BOARD
KEYPAD
ELECTRICAL DIAGRAMS 4-3
Mains input -
R143
Steady state condition 20R
Masse V35
Max Power = 500W
Imax@120Vac=6Arms(max)
C87 C86
Imax@230Vac=3Arms(max) 1uF X2 2nF2 - Y2 L15 V36 US1J
7
2
8
1
275 VAC N.M. 2x7.3mH GBPC1508W
9.3A TP18
K1B ~
X16 1 2
Mains Input 62.22.9.024.0300 + U+
U
1 -
2 R144 K5A
3
6
4 7 ~ C15 R145 R146
V275LA40A 2nF2 - Y2 33k
Lumberg K46 40.61.7.024.0000 75K
3 4 6 9 250VAC
C88 Z1
VR C89
2nF2 - Y2 R147
+
N.M. K5B 1200uF 33k
40.61.7.024.0000
4
75K R151
8
DC+
R152 R153 R150 33k
+ 3 DC-
150K 150K 1 R154 R149 470K C91
- 2 10k 10k 100nF
5
C90
+
M4 mounting V37
1uF 500V R156
BAS16
4
1 LM393 450V 33k
D9B R155 R157
Z6 470K
8
75K
V38 + 5
7 R158
BC817-25 C92 33k
+
K2B - 6
1200uF
PCJ-105D3M high to C90 > 200V 250V
C93 R159
+
Masse NK1
4
47F 12k
V39 25V R160 V41
V40 TP19 LED red
3 BAS16 75K
TL431A
2 U-
NK2 5 C94
3 1
X17
Lumberg K37
K3B
PCJ-105D3M
to supply 2 section SW2
Netz 2pol
Z1
2nF2 - Y2
250VAC
6
page 4-4
1
4 2
condensor Z1
3 3 M4 mounting
2
fan 1 N26119048
2 +24V
N C95
SELV
PCJ-105D3M
K2A 100nF +24V
1
V42 50V
DC+ X9
ZK
BAS16
DC- OT Brake Resistor
4
2
GND 1
X18 R161 fan1
Lumberg K15
overpressure V43 4k7
2 K1A
V275LA10P BC817-25
1
switch 62.22.9.024.0300
to controller section
A
R163 GND
R162
DC+DC- V44
C14
R270
U
R271
page 4-7 ES1D 100R
PCJ-105D3M
K3A
B
1
47R
2
1
2 R165 K6B
compressor
1 3 PCJ-105D3M
8 R166 fan1 high speed 10K
D28
R164 LTV-357T
6 V46 4k7 10k
BC817-25
3
2 R292
GND TP16 10K V47
7 V48 BZX84C5V1
K4A Conductor status
BC817-25
40.61.7.024.0000
40.61.7.024.0000
K4B R170
4
V49
DC+ 2K2
BAS16 R169
DC-
4K7
5
TP17
4k7
V50 REL_ON_Buff
GND
VCC
V51 R174
R172 R173 BZX84C5V1 1k
R175 VCC +24V
22k 22k 100k
5
C96 +24V
/overpressure
10nF 2 4
50V
R178 VCC
R246
1M C97 D29 0R
3
N ZK
R217
PTC 6R C840 Pmax(tot) = 18W
NK2
V57
GBU404 Iout = 0.05A
~ VR D37 TP11
L16
LM317 22u@2.13A Iout = 0.12A
- + R242
3 2 +15Vprim
R218 R219 VI VO
ADJ
~ 1K2 1K2 0R
R300
C123 3K9
NK1 C119 R222 C120
+ C121
1
3n3 V77 C122 + C124
R220 R221 250Vac 1N5362B 100uF 47uF 100nF
100nF 249
V58 V59 1K2 1K2 Y1-Type 5W 50V 100nF 35V 50V
50V V78 TP12
R223 50V
N.M.
LED red
P6SMB200A
V60 2K87
T1
R224 TRAF_B66208W 7 U-
R225
1K2 1K2
ES3D TP13
C126 C125
+
L17 R243 Iout = 0.5A
330F
3 +24V
35V
V61 68pF 22u@2.13A 0R
US1J C127 R301
500V 6
4 10nF V79 5K6
50V
V62
10 C110
ES3D 100nF LED Green
V63 R228 R229 5 R230 GND
BZX84C30 5K1 4R7 V64 R231 D38 50V
R226 R227 C129 C131 C132
V76 4K7 A8499
+
2 TP14
33R C128 1 8 L18
680u 100nF 100uF Boot Vin GND
2M 2M C130 + C140 ES1D 470pF 22u@2.13A R244 Iout = 0.5A
0,25W 0,25W 22uF 100nF 200V 35V 50V 50V 2 7
ENB LX VCC
50V 1 9
0R
3 6
1N5362B R234 TSET VBIAS
R232
PAD
C152 5W 36K5 4 5
GND FB
R249 (N.M.) C150 R233 R299
(N.M.) R250 470nF(N.M.) 475R 10K 1K
9
56R (N.M.)
C133
33nF V75
D39 D40 LED Green
TOP255YN LTV-357T R235 R239
R236
R238 3K16
7 1K5 10K
+
DRAIN R237 30K V65 C135 C134
4
SOURCE 4K75 100nF
SS34 470uF
MV2 N15468022 50V
CONTROL
10V
Frequency
N.M.
I-External
V-Monitor
R240
6R8 V66
TL431A TP15
5
3
2
1
C137
100uF
GND
400V
C136 R241
+
1
Z2
M4 mounting
SELV
Power Supply 2
ELECTRICAL DIAGRAMS 4-5
+15V +15V-W
+15V
C32 + C33
100uF 100nF
50V 50V
8 24 U+
VP1 P
C34
R50 100nF DZ2
330R D15 C38 R55 1SMA5934BT3
D16 24V
1 6 100nF 10R
VCC 2
R52 2 5 VUFB
C35
4K7 MURS160T3 10uF + C39 C40
3 4 R53 35V 100nF
D18 0.22F
C42 10R
R56 3 630Vdc
100nF ACPL-P480 VVFB
330R D19 C36 polyprop.
50V GND DZ3
1 6 MURS160T3 10uF + C43
R71 R58 24V 35V 100nF
R57 D20
4K7 2 5 10R
GND D41 4K7 1SMA5934BT3 4
74HCT365 3 4 C44 VWFB L5
16 C45
100nF MURS160T3 DZ4 10uF + C46 1K 100MHz
PWM-UP 2 3 R59 24V 100nF
ACPL-P480 35V
Vcc
4 I1 O1 5 330R D21
PWM-VP I2 O2 GND R60 5 4 +15Vprim
PWM-WP 6 7 1 6 1SMA5934BT3 5 D45 23 6 3
10 I3 O3 9 UP PS21964-AT U
PWM-UN I4 O4 330R R62 7 2
PWM-VN 12 11 R61 2 5 6 8 1
14 I5 O5 13 VP motor temperature sensor
PWM-WN 4K7 330R R63
I6 O6 3 4 C47 7
1 100nF WP 22
GND
DA R64 330R V
15 ACPL-P480 C50 C48 C49
DB 330R D22 100pF 100pF X11
GND 100pF
1 6
2
8
CIN
+15V snap-rivet snap-rivet
GND
R83
VCC R84
15
R86 1M
10K 470R
D25 R87 C59
R85 ACPL-M50L Z5 (contact to frame (earth))
1
1nF R88
4K7 50V 0R033 M4 mounting
/inverter fault V8 150K CER 2W
BC807-25
R89
U-
4K7
GND
SELV ZK
Frequency Inverter
ELECTRICAL DIAGRAMS 4-6
U+
R182
470K
8
+ 10uF GND GND
R194 3 + 680R V53
35V
10K 1 SPP20N60C3
2 - V54
R195 TL431A
10K
4
R197
C102 R198 R196 C104 5K6
V55 10uF 6K8 68K 22pF C103 C101
TL431A 6,3V 50V GND 1uF 1uF
10V 10V
U- TP25
overtemperature motor
V56 D6B
BAT54 MC33072ADG VCC
5 +
7 D49
SN74AHC1G08 TP24
5
6 -
1
Vcc
R200 A 4
out /overvoltage
3 GND
10K 2
link discharge R202 R204 B 420V (Typ)
4K7 4K7 C148
R201 100nF
750k up 210V down 205V 50V GND
VCC
VCC
GND C106
C105 R205 R206 100nF
100nF 10K 50V
R207 C107 2M2 power low
8
50V
750k 100nF 3 + GND
50V 1
2 - D34
LTV-357T
R209 D7A
4
18k LM393
R212
GND
10K
8
5 +
7
6 -
D7B
4
LM393
ZK SELV
Protection Circuit
ELECTRICAL DIAGRAMS 4-7
R4 R5
SELV
+3.3V 4K7 4K7
VCC D10 +3.3V C6 C7 C8
C2
LP3985 3.3V 100nF 100nF 100nF 100nF
1 5 50V 50V 50V 50V R3 R313
VCC Vin Vout 4K7
14
C5 C16 C12 4K7 D2A
3 4 100nF
Ven Byp C3
GND
100nF C4 100nF C1 74HCT14 compressor
50V 10nF 50V VCC C11 100nF 50V
10uF GND compressor_CMD 1 2
50V 10V 100nF 50V
2
/overvoltage 50V J1
D42 12.0 MHz VCC
14
7
SN74AHC1G08 D2B
5
GND
1 74HCT14 fan1
Vcc
28
54
71
96
42
84
19
/Inverter fault VCC A 4 Fault /fan1 3 4
out C13
3 GND
2 C18 100nF
VDD3.3_reg_1
VDD3.3_reg_2
C17
VDD3.3_1
VDD3.3_2
VDD3.3_3
VDD3.3_4
B
VBAT
22pF R314 50V
C143 22pF 50V 4K7
14
14
50V
7
D1F 100nF D4 D2C
74HC14 50V C9 C10 18 22 SN74AHCT1G00D 74HCT14
RTCX2 XTAL1 23 GND GND GND fan1 high speed
1nF 1nF
5
power low 13 12 GND GND 16 /fan1 high speed 5 6
50V 50V RTCX1 XTAL2 1
46 17 /RST 4
14
47 P0.0_SDA1 \RESET 14 D47F 2
14
GND GND P0.1_SCL1 \RSTOUT
7
7
TXD0 98 74HCT14 /Enable Gate D2F
14
3
81 94 REL_ON 13 12
3 4 80 P0.4 P1.1 93 /fan1
79 P0.5 P1.4 92 /fan1 high speed
P0.6 P1.8 GND
7
overtemperature motor D1B 78 91
P0.7 P0.7 P1.9
7
74HC14 77 90 Fault-SM VCC
P0.8 P0.8 P1.10
14 7
P0.9 76 89
D1A 48 P0.9 P1.14 88 R321
/EN /Curr-reduc
14
74HC14 49 P0.10 P1.15 87 4K7 D47D
Conductor status P0.11 P1.16
1 2 SCLK 62 86 74HCT14
P0.15 P1.17 /DIR PWM-Buzzer
63 32 9 8
61 P0.16 P1.18 33
P0.17 P1.19 PWM-UN
MOSI 60 D11 34 PT1000_ON VCC
P0.18 P1.20
7
/CS1 59 35 Fault
14
7
D1D 58 LPC1764FBD100 36 PWM-UP R312
P0.20
14
14
74HC14 57 P0.20 P1.22 37 Display On-Off D47E D3E
P0.21 4K7
9 8 56 P0.21 @40MHz P1.23 38 74HCT14 74HC14
lid closed P0.22 P1.24 PWM-FAN
V9
1
9 39 PWM-VN 11 10 11 10
C144 R7 +3.3V P0.23 P1.25 MCP9701A
8 40
VDD
3K3 P0.24 P1.26 PWM-VP
100nF R6 7 43 2 out C25
NM P0.25 P1.27
7
3 GND
6 44 PWM-WN
P0.26 P1.28
7
25 45 PWM-WP 50V VCC R268
D12A M24C128WBN6 CX1 24 P0.27 P1.29 21
R8 P0.28 P1.30 imbalance 33R
8-pin Socket D12 R253 4x470pF GND 29 20
1 8 3K3 3K3 30 P0.29 P1.31
GND C149
E0 VCC 7 P0.30 H2A
2 GND 75 TXD 100nF TSAL7200
E1 /WC 6 /REL_SM P2.0 IR Socket
3 74 RXD 50V 940nm
4 E2 SCL 5 compressor_CMD 26 P2.1 73 FAN
14
VSS SDA 27 P3.26 P2.2 70 D47C H2
P3.25 P2.3 /Steps GND R269
69 74HCT14 1K
14
/RST /overpressure P2.4 68 D1E 5 6 V67
GND TP27 P2.5 P2.5
D13 R252 82 67 74HC14 Stepper Motor Reset AO3414
+3.3V P4.28 P2.6 P2.6
MCP1319MT-29 0R REL_ON 85 66 10 11
P4.29 P2.7 P2.7
5 1 65 P2.8
Vcc RESET P2.8
7
50V 64
Reset 5V P2.9
100nF 3 Reset 5V 13 53
GND
GND
14
RESET N.C. P2.10 GND
7
C21 4 52 R264 VCC
MR P2.11 rpm
RTCK 100 51 Rotordetection lid lock closed 100R X21
GND TDO/SWO 1 JTAG_RTCK P2.12 50 6 5 4
JTAG_TDO P2.13 /LID CURR 4
2
TDI 2 3
_MR TMS/SWDIO 3 JTAG_TDI D1C 2 3
GND JTAG_TMS +3.3V 2
TRST_N 4 74HC14 R265 C147 1
\JTAG_TRST 1
7
TCK/SWDCLK 5 100R 100nF
SW1 JTAG_TCK 50V Debug Interface
N.M. C24
100nF GND
GND D14 C27
VREFN
VREFP
50V
16
VDDA
VSSA
VSS1
VSS2
VSS3
VSS4
VSS5
VSS6 C26 ST3232C 100nF
GND
100nF 4 1
Vcc
L3 +24V C2+ C1+
1K 100MHz V5 50V 5 3 50V
31
41
55
72
83
97
10
15
12
11
15
4K7 R36 +3.3V +3.3V
1K@100MHz 4K7 R28
GND 47R GND GND GND
PT1000_ON R37
Display On-Off R44
+3.3V JP3 JP1 R322
R25 R26 R27 R15 R16 47K V7 JUMPER 22K JUMPER 22K
+3.3V 10K 10K 10K 10K 10K
BC817-25 2 1 _MR 2 1
GND R43 R42
14
14
14
1 GND GND
10 9 TCK/SWDCLK D48C D48D GND 2
+ + 2 Pin2 +5V
12 11 RTCK +3.3V 74HCT14 74HCT14 R47 100R 3
+ + 3 Pin3 SCLK
14 + + 13 TDO/SWO MOSI 5 6 9 8 R46 100R 4
/RST 5 4 Pin4 GND
16 + + 15 5
R45 100R 6
shield0
Pin5 MTSR
shield1
18 + + 17 6
20 19 R35 R315 7
+ + 7 Pin6 GND
7
14
9B
GND
/CS1 13 12 11 10 Shield GND
GND GND GNDGND
GND GND
Controller
7
7
ELECTRICAL DIAGRAMS 4-8
PCJ-105D3M
VCC
+24V
K7A
1
V18 DC+
C168 BAS16
VCC D31 DC-
100nF
rpm SN74AHCT1G08 D8
4
SN74AHCT1G08
3
/Reset 5V 1 GND
Vcc
VCC A 4 1 R294
Vcc
C60 out A
3 GND
R92 2 4
100nF R93 B out
3 GND
220K 2
R90 D5A 50V 1M D5B B 4k7 V20
33K 74HC4538 74HC4538 BC817-25
K7B
16
GND
2
imbalance N5469480 PCJ-105D3M
GND
C64 2 C65 14
VDD
C61 R91 1uF RC 6 1uF RC 10 REL_SM
R261
10nF NM 10V 1 Q 10V 15 Q
33K GND
50V R248 CX CX VCC
33K 4 12
5 +T 7 11 +T 9
Vss
-T Q -T Q
R
X12 L7 C63 R94 C170 R295
TP68
JST stehend 1K 100MHz 100nF 1K R99 100nF R100 0R5 VCC
13
14
GND GND
8
1 4 5 D2D 4K7 50V 3K6 D35 1W
1 2 3 6 74HCT14 N5468836 3 1 GND INA193
2 R298
3 2 7 9 8 1 5
3 R98 N5468902 V10 OUT V+
4 1 8 R297 2
4 L8 R95 10K BAS16 270K GND C169
8
27K 3 4
100mH 1K C67 R97 Vin+ Vin-
SPEED + + 3 100nF
7
R96 100nF 1K GND /LID-CURR 1 GND 50V
C66 100k 50V GND GND 2
UNBALANCE GND VCC - GND
1nF D52A
R302
50V LM393
C76 15K C111
4
V11 100nF R101
8
GND GND GND GND GND 10nF
50V 1K2 C160 220nF
LED Green + 5 50V
7
C68 6
- V1 V15
10uF TP67 GND
X13 L11 6,3V C77 +
R293 R291 VCC VCC D52B BAT46W BAT46W
14
4
JST stehend 1K 100MHz R102 D2E LM393 C161 100uF
4K7 4K7 GND
1 4 5 1K 74HCT14 C71 220nF 50V
1 GND
2 3 6 11 10 100nF
2 C159
3 2 7 50V
3 L10 10nF SH1
1 8 R296 R251
100mH 50V
4K7 4K7 GND GND GND_1
14
GND 18
GND 19
15
22
20
17
7
6
GND 7
VBOOT
100k 100nF R107 74HCT14
GND
VCP
VSA
VSB
C73 50V R106 1 2 1
1nF CLOCK
1K L20
50V 100R C74
1nF 300R@100MHz
GND GND GND GND 2 5
CW/CCW OUT1A
7
50V
One step ON
Rotordetection GND 21
each falling edge 14 D51 OUT2A
GND EN X8
3.3V L21
VCC L6228D 300R@100MHz 1
14
F = 150-800Hz 0V D43B 23 L22 2 1
/Steps RESET 300R@100MHz 2
74HCT14 8 3
3 4 OUT1B 4 3
/DIR 4
13 16
14
Low = Motor current ON /LID-CURR D43C CONTROL OUT2B
/LID CURR
SENSEA
SENSEB
74HCT14 L23 JST stehend
VREFB
VREFA
R287
7
Low = drive enabled 5 6 12 300R@100MHz
RCA
RCB
/EN H/F
14
Low = Relay closed D43D 100K
/REL_SM
74HCT14
24
10
11
7
3
Low = No Fault 8 9
Fault-SM R284 R283 R279 C164
R289
14
D43E 1K8 1K8 100K 820pF R282 R281
74HCT14 100K
C75 C166 C165
7
11 10 /Reset 5V R280 1R 1R
5n6F
VCC 100K 1W 1W
14
D43F 50V 220nF
VCC 74HCT14
7
13 12 REL_SM C167 820pF
D46 220nF R103 GND GND_1
R116
5
1K8
1 4K7 FDN304P
Vcc
Reset 5V A
7
4 L12 V13
out V21 X14 V12
GND
6 C112 C113
R307 7 2
C79 4K7 4K7 10k 6 3 VCC 820pF 820pF
1nF R121 VCC 5 4 2 1 Lid Switch
50V 10K L4
GND 1K 100MHz VCC C114
K6A 100nF
R266 V22
10k GND
R117 R118 GND BAS16 DC+DC-
R11 GND
5
lid closed
R305 R306 4K7 1
C78 4K7 4K7
V81 /Curr-reduc 4
1nF BC817-25 2
50V 100k 100k R308
10k D26
GND
3
SN74AHCT1G00D
Stepper Motor Reset
C173
R309
4K7
R310
4K7
R311
10k
GND
VCC
SELV GND
1nF
GND 50V GND
Imbalance RPM
ELECTRICAL DIAGRAMS 4-9
V27 BAT54
+3.3V V29 BAT54
R317 V25 BAT54
V28 BAT54
4K7 V30 BAT54
V26 BAT54
R316 R318 V24 BAT54 L13 1K 100MHz
4K7 4K7 V23 BAT54 L14 1K 100MHz
R122 1K D27
R124 1K 74HCT138
VCC 1 15 3 1 1 8
P0.7 A Y0
P0.8 2 14 3 1 2 7
B Y1 1
P0.9 3 C Y2 13 3 3 6
12 3 1 4 5
R123 Y3 1
1K Y4 11 3 1 8
VCC 6 10 3 1 2 7
G1 Y5 1
4 G2A Y6 9 3 3 6
5 7 3 1 4 5
Reset 5V G2B Y7
GND C80
100nF
10
12
14
50V
2
4
6
8
GND
GND VCC X15
+
+
+
+
+
+
+
GND connecting plug cod
+3.3V +24V
+
+
+
+
+
+
+
13
11
1
3
5
7
9
R126
R319 R125 27R C41
4K7 4K7 V31 3.3V 100nF
14
BC807-25 50V
0V D17A GND
V32 MC33072ADG V2
8
P0.20 1 2 R1
BC807-25 3 + BC817-25
PWM-Buzzer R17 C52
D47A R127
+
LEDblock1 100K 1 1uF
74HCT14 4K7 (Fpwm = 4KHz - 2 - 1K 50V
7
4
+3.3V 100nF
50V +
R129 75K H1
R320 R128 27R R12 SMA-17
4K7 4K7 V33 10K
14
BC807-25 -
3 4 V34
P0.21
BC807-25 GND
D47B R130 LEDblock2
74HCT14 4K7
7
VCC
R131
100k
14
50V V3
GND 5V STD12NF06L 200R 300MHz
R22 FAN
GND
VCC 0V 5 + 1K
VCC 7
R20 C145 C151
PWM-FAN 6 - +
C81 D17B
100nF R134 (Fpwm = 4KHz - PWM 10K MC33072ADG 100uF 100nF
50V 100k 100% high = FAN OFF) R21 50V 50V
14
C83
MMSZ5242B
1nF
12V-0.5W
7
50V GND
GND
GND
VCC
R137
100k
14
14
C84 D3F
1nF 74HC14
7
50V 13 12
GND
7
VCC
GND
R140
100k
14
50V
GND
Key Control
DISASSEMBLY OF INSTRUMENT PARTS 5-1
Before disassembling any parts, switch off the unit and pull out the mains
plug.
DISASSEMBLY OF INSTRUMENT PARTS 5-2
Open the lid, switch off the unit and pull out the mains plug
Remove the 2 screws on top of the front panel
Pull the front panel up and lift it off.
Disconnect wiring from display and overlay adaptor board
Back panel
Switch off the unit and pull out the mains plug.
Remove the 7 screws attaching the back panel to the casing.
Tilt the panel down and unplug the cables.
Lid
Open the lid, switch off the unit and pull out the mains plug
Remove gas lid stay from top joint block
Remove the 4 screws attaching the lid hinges to the main
frame.
When reassembling the lid take care that the lid bolt fits the
casing hole centric.
42mm
DISASSEMBLY OF INSTRUMENT PARTS 5-3
Gasket
Bowl
Casing
Before replacing the NV-RAM enter the system menu and note
the version and revision number.
Remove the front panel.
Remove the 4 screws at the swivel cassis and flip down the
main board.
ATTENTION - Discharge your body before handling
CMOS components!
Use a chip removal tool or a small screwdriver to pull the NV-
RAM out of its socket.
After reassembly check the version and revision number of the
new NV-RAM in the system menu.
Main board
Lid latch
LCD
Keypad
Remove the front panel and flip down the main board.
Disconnect the brake resistor and its over temp. cut out from
the main board (X20 and X9).
Remove the back panel.
Remove the 2 screws attaching the brake resistor to its holder.
Attach a string to the cables before pulling them through.
Remove the brake resistor.
Use the string to pull the cables of the new brake resistor
through.
After reassembly check the function of the brake resistor during
a short test run (resistor must heat up during braking
CAUTION it may be hot!).
DISASSEMBLY OF INSTRUMENT PARTS 5-6
Removal of AutoLock
This work requires AutoLock tool kit 70904693 or similar
tools.
Removal of AutoLock
Use the puller supplied with the tool kit to pull off the AutoLock
from the motor cone.
The inner and outer tapered surfaces of the Autolock must not
show signs of damage such as scratches or dents.
Check the correct un-locking with the adaptor and the AutoLock
pin supplied with the tool kit.
CALIBRATION (REFERENCE MEASUREMENT) 6-1
Introduction 6-2
General 6-2
Requirements 6-2
Equipment 6-2
Documentation 6-2
Preventive Maintenance 6-2
Documentation 6-5
CALIBRATION (REFERENCE MEASUREMENT) 6-2
Introduction
This chapter describes the calibration work to be performed on a yearly basis.
A calibration certification form can be found in the attachment B-1.
General
Successfully completing the preventive maintenance checklist and calibration procedure as outlined
will ensure that the instrument is properly maintained and calibrated to Thermo Scientific
specifications.
Requirements
Equipment
Note: Check calibration due date on all test equipment before starting the procedure.
Documentation
Service manual
Centrifuge operating manual
Preventive maintenance checklist (A-1)
Calibration certification form (B-1)
Certification label
Certificate of calibration for each piece of test equipment used to perform the calibration
Training certificate
Preventive maintenance
Perform preventive maintenance checks as outlined in the service manual to ensure the
instrument is in good working order before performing any calibrations.
Complete the preventive maintenance checklist. (A-1)
CALIBRATION (REFERENCE MEASUREMENT) 6-3
Install a rotor and attach a piece of reflecting tape (or black tape
on reflecting rotors) near the center of the rotor. Close the
centrifuge lid.
Set the speed to 1000
000 rpm and start the centrifuge. When the
display shows 1000 rpm wait for 10 seconds. Use an optical
(laser) tachometer and measure the speed of the rotor through
the window in the centrifuge lid. Record the measured speed and
the displayed speed in the speed section of the certification form.
Set the speed to rotor maximum.
imum. When the display shows the set
speed wait for 10 seconds and measure the speed through the
window. Record the measured speed and the displayed speed in
the speed section of the certification form.
Determine whether the speeds recorded meet the specifications
on the form. If the specifications are met, this test is finished. If
the specifications are not met, replace the main board, as
adjustments are not possible and repeat the test.
Time
Install a rotor and close the lid. Set the speed to 1000 rpm and
the time to 10 minutes. Start the centrifuge and the stopwatch at
the same time. Stop the stopwatch when the time section of the
display shows 0. Record the measured time in the time section of
the certification form. Determine if the time value recorded meets
the specifications in the form. If the specifications are met, this
test is finished. If the specifications are not met, replace the main
board, as adjustments are not possible and repeat the test
CALIBRATION (REFERENCE MEASUREMENT) 6-4
Imbalance
Install a rotor. Place the rotor dependent cut off weight in one of
the buckets / cavities and close the centrifuge lid. Set the speed
to rotor maximum and press start. The centrifuge must shut off
with an imbalance alarm sometime during acceleration.
Repeat this test in 3 more positions, each displaced by 90.
Repeat the test with rotor dependent run through weight.
Determine whether the cut off and run through weights meet the
specifications in the service manual and record the rotor type, cut
off and run through weight in the imbalance section of the
certification form. If the specifications are met, this test is
finished. If the specifications are not met, replace the main board,
as adjustments are not possible and repeat the test
Safety test
Documentation
After filling in the certification information complete the following documentation:
SPARE PARTS
Drive 7-6
Spare part numbers and spare parts are subject to change in case of technical upgrades or
supplier change. New specifications and part numbers are announced through technical
service bulletins.
SPARE PARTS 7-2
Front panel
Keypad
Front view
20059092
Brake resistor
NV-RAM
Back view
20059092 20059098
Brake resistor Gas spring
20059189
Gas spring
coupling
70903021
Lid Assembly
20059071
Hinge
20059077
Foot
SPARE PARTS 7-5
Top view
70903021
Lid Assembly
20059071
20059098 Hinge
Gas spring
20059004
Bowl
20058389 70902026
Gasket AutoLock
70903034
Sensor cover
20059014
Front panel
20150316
Display
Keypad
Drive
70902026
AutoLock
70903034
Sensor cover
20059030
Motor
70902001 incl.
3x AV-mount
6x sand paper disc
Preventive maintenance Megafuge 8
SL8 / ST8
checklist
Account name: Account address:
Instrument model: Serial number:
Report number: Date:
1 of 2
Report Number: Date:
Imbalance detection
Cuts off with cut off weight
Does not cut off with run through weight
Comments:
Braking circuit
Brake resistor cold in stand by
Brake resistor hot during deceleration
Brake effect smooth and noiseless
Comments:
Comments:
Use ONLY blue permanent ink when filling out. Leave NO blank spaces. Oct, 2010
Revised 10/07/12
2 of 2
Megafuge 8
Calibration certification ST8 / SL8
Account name: Account address:
Instrument model: serial Number:
Report number: Date:
SPEED
Standard test Rotor used:
Set speed Tolerance Displayed Measured Meets Passed
Notes
(rpm) (whichever is greater) Speed (rpm) speed (rpm) spec. (y/n) (initials)
1000 +/- 10 rpm, 1%
Rotor max +/- 10 rpm, 1%
Custom test Rotor used:
+/- 10 rpm, 1%
+/- 10 rpm, 1%
IMBALANCE
Rotor used: 0 90 180 270
Rotor cut off weight: Cuts off (y/n):
Passed (Initials):
Rotor run through weight: Runs through (y/n):
Passed (Initials):
TIME
Standard test Rotor Used:
Timer Set Tolerance Measured Meets Passed
Notes
(min.) (sec.) time (m/s) spec. (y/n) (initials)
10 +/- 10
Custom test Rotor Used:
+/- 10
+/- 10
SAFETY TEST
Limit Actual Passed (initials)
Grounding resistance < 200 m
Insulation resistance > 10 M
Accessible current < 500 A
TEST EQUIPMENT
Type Model Serial number Date of next calibration
Stopwatch
Temperature meter
Optical tachometer
Safety tester
Use ONLY blue permanent ink when filling out. Leave NO blank spaces. . June, 1999
Revised 10/07/12
X:\DEOHA\Public\TS\SM\Ueberarb\MISTT\Manual Dateien\B1-MISTT calibration form.docx
TECHNICAL BULLETINS C-1
TECHNICAL BULLETINS