ANTIBACTERIAL ACTIVITY OF Jatropha Curcas L.

ROOTS
METHANOL EXTRACT AGAINST Staphylcoccus Aureus

Dian Puspitawarni _1508062235

Faculty of Pharmacy Ahmad Dahlan Yogyakarta
 Abstract
ABSTRACT
ANTIBACTERIAL ACTIVITY OF Jatropha curcas ROOTS METHANOL
EXTRACT AGAINST Staphylcoccus aureus
Dian Puspitawarni, Muhammad Muhlis
Faculty of Pharmacy, Ahmad Dahlan University
Correspondence :
dianpuspita36@gmail.com
Background: Antimicrobial is substance that can kill/inhibit the growth of pathogenic microorganisms. One of
microorganism that can cause infection is Staphylococcus aureus. Now, there are increasing resistance to many
antibiotic, so scientist encouraged to find new antimicrobial from a variety source including medicinal plants. One of
the herbs that can be used as an antibacterial agent is Jatropha curcas L. Based on previous research showed
antibacterial activity from J. curcas.
Objective: To determine antibacterial activity methanol extract of Jatropha curcas L. against S. aureus based on
research thats been published.
Metode: This study used Systematic Review method. The inclusion criteria: article in English, published in 2006 -
2016, full-text article or abstract has result data, the species used is J. curcas, using methanol extract of Jatropha curcas
root, studied use Staphylococcus aureus bacteria. Exclusion criteria: incomplete publication, not as an antibacterial
effect, there is no MIC values and clear zone.
Outcome measured: MIC and minimum inhibitory zone
Result: Methanol extract of J. curcas has antibacterial activity against S. aureus with MIC 2,3 ; 6,25 ; 30,82,50 mg/ml
and minimum inhibitory zone 14 ; 110,24 ; 29,60,23 mm.
Conclusion: Based on a review article showed that methanol extract of J. curcas root has antibacterial activity
against Staphylococcus aureus, so J. curcas root has potential as antimicrobial source from plant. MIC values showed
no correlation with the minimum inhibitory zone, it caused by some factors that influence so need to standardization
of simplisia.
Keyword: Antibacterial, Jatropha curcas L, root, methanol extract, Staphylococcus aureus
 Background
Antimicrobial is
There has been an increasing
substance that can kill/
interest in use of natural bioactive
inhibit the growth of
compound for medicine resistance
pathogenic
to many antibiotic
microorganisms

Extracts from various parts of

Jatropha curcas L. showed
bactericid activity (Igbinosa et al.,
2009)
Objective

The systematic review aimed to determine

antibacterial activity methanol extract of
Jatropha curcas L. against Staphylococcus aureus
based on research thats been published.
 Method

Searching Data
PICO
P : Antibacterial
Selection & Result
www.google.com I : Methanol extract of
Inclusion: article in English,
www.scholar.google.com Jatropha curcas L. root
published in 2006 2016, full-text
C : Placebo
O: Antibacterial activity
article, the species used is J. curcas,
methanol extract of Jatrophausing methanol extract of Jatropha
curcas L. root against S. curcas root, studied use
aureus Staphylococcus aureus bacteria

Exclusion: incomplete
publication, not as an antibacterial
effect, there is no MIC values and
clear zone.
Selected 3 article
Outcome

Methanol extract of
Jatropha curcas. L
root
(Jarak Pagar)

Antibacterial

MIC and Clear Zone

 Result & Discussion
Tittle Result
Phytochemical Compound and Methanol extract of Jatropha curcas root showed
Antibacterial Activity of antibacterial activity against S. aureus with the MIC
Jatropha curcas Linn. Extract 2,3 mg/ml and clear zone 14 mm
Comparative In Vitro Methanol extract of Jatropha curcas root showed
Antimicrobial and good antimicrobial activity against S. aureus by
Phytochemical Evaluation providing clear zone 11 0,24 mm and the MIC value
Methanolic Extract of Root, 6,25 mg/ml.
Stem and Leaf of Jatropha
curcas Linn.
Antimicrobial Potential of Methanol extract of Jatropha curcas root showed
Methanolic Extract and antibacterial activity with the clear zone sebesar 29,6
Various Fractions of Jatropha 0,23 mm and the lowest MIC value 30,8 2,50
curcas Root mg/ml.
 Result & Discussion
Minimum Inhibition Minimum Inhibitory Zone
Article
Concentration (MIC) (Clear Zone)

1 2,3 mg/ml 14 mm
2 6,25 mg/ml 110,24 mm
3 30,82,50 mg/ml 290,23 mm

Methanol extract of Jatropha curcas L.. root showed antibacterial activity

against Staphylococcus aureus
There is no correlation between the MIC value with the minimum inhibitory
zone
 Conclusion

Based on a review article showed that methanol extract of

Jatropha curcas L. root has antibacterial activity against
Staphylococcus aureus, so it has potential as antimicrobial
source from plant. MIC values showed no correlation with
the minimum inhibitory zone, it caused by some factors
that influence so need to standardization of simplisia.
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