Professional Documents
Culture Documents
Rev. AA
This Service Manual is copyrighted and Beckman Coulter reserves all rights. This
document may not, in whole or part, be copied, photocopied, reproduced, translated, or
reduced to any electronic medium or machine-readable form without prior consent, in
writing, from Beckman Coulter, Inc.
RELATED DOCUMENTS
This service Manual does not include the Operator's Manual for this instrument. The
Operator's Manual contains instructions for proper operation of the instrument,
information regarding the limitations, specifications, precautions and hazards associated
with its use.
To obtain a copy of the Operator's Manual(s), please order it through any Beckman
Coulter Sales and Service Office.
Specified Warnings:
WARNING: The use of parts not designed or documented for this product is not
authorized. The use of non-Beckman Coulter parts may cause damage to
the product or personal injury to operating personnel. The use of these
parts will also void any warranty.
WARNING: The bolts for the anchoring bracket are critical and classified as
Class 10. Only those bolts are allowed to use.
IMPORTANT NOTICE
This Service Manual is designed solely for the use of Beckman Coulter Field Service
Representatives who have had training and experience in the servicing of Beckman
Coulter products. To improve product performance, Beckman Coulter may have made
changes in the product to which this Service Manual relates. Such changes may not be
reflected in this manual. Accordingly, Beckman Coulter makes no representation or
warranty, either express or implied, that the information contained in this Service Manual
is complete or accurate with respect to the particular product to which this manual
pertains, and the purchaser must assume all risks in the use of this manual for the purpose
of performing service upon such product.
Do not replace any centrifuge components with parts not specified for use on this
instrument.
Components of this instrument which are considered user serviceable are discussed in
detail in the instrument's instruction manual.
CAUTION:
The use of parts not designed or documented for this product is not
authorized. The use of non-Beckman Coulter parts may cause damage to the
product or personal injury to operating personnel. The use of these parts will also
void any warranty.
WARNING:
The bolts for the anchoring bracket are critical and classified as Class 10.
Only those bolts are allowed to use.
MECHANICAL SAFETY
l. Remember to keep clothing and fingers away from rotating or moving components.
It is easy to slip, lose a tie, or get cut while working near one of these devices.
3. Always use the tools specifically recommended for the job. This not only reduces
the chances of injury, it minimizes damage to the instrument.
5. After the mechanical repairs have been completed, always check that all mounting
hardware and safety shields are in place and secure. By doing so, you will not only
ensure safe operation of the instrument, but may reduce callbacks.
As you can appreciate, all it takes is good common sense, plus good safety techniques
that are basic and easy to remember. With this is mind, you should have no trouble
servicing mechanical devices safely.
ELECTRICAL SAFETY
l. Avoid standing on damp floors! People have been shocked simply by standing on a
wet floor and then working on live equipment.
2. Be sure to disconnect the power cord before working on any high voltage circuitry!
As power switches disappear from new equipment, this precaution is very easy to
overlook. Attach probes for checking voltage and current with power off!
3. Read and heed ALL caution labels! They are posted for YOUR safety.
4. Take care of test equipment. Be sure to use the right probes for the right job.
Measuring high voltages, for example, requires a well-insulated, high voltage probe.
Damaged probes and cables are dangerous and should not be used. Also, be cautious
around test equipment such as an oscilloscope. The oscilloscope case may become
live if you connect the cable ground to a live circuit. Avoid this dangerous situation
at all times!
5. Always treat cathode ray tubes (CRTs) and uncased photomultiplier tubes (PMTs)
with special care. When carrying them, wear eyeglasses and protective gloves. If
these devices are dropped or struck with a sharp object, they can implode violently
and scatter glass.
These basic tips regarding good electrical safety practices are easy to remember.
Combined with good common sense, they should keep you on the job for a long time to
come.
ELECTROSTATIC DISCHARGE
Static electricity is an electrical charge which
can damage circuitry and components.
Listed below are important Electrostatic
Discharge guidelines for handling electronic
components. Following these guidelines will
avoid needless damage to circuit cards and
components on them.
Keep paper, non-conductive plastic, plastic foams or cardboard away from circuit
boards and components and static-free work areas.
Keep hand creams and food away from the conductive work surfaces. If spilled on the
bench top, these materials contaminate and increase the resistivity of the work area.
Be especially careful when using soldering guns around conductive work surfaces.
Solder spills and heat from the gun may melt and damage the conductive mat.
Check the grounding connections on wrist strap and static mats. (Mat must have proper
ground.) Make certain they fit snugly before starting work with the components and
printed circuit cards.
Do not allow anyone not grounded to touch ESD sensitive components in the work area.
To be grounded, they must be standing on the conductive floor mat and must touch the
conductive bench top mat before touching the components or printed circuit cards.
Do not place ESD-sensitive components on work areas that are not grounded properly.
Do not touch the component by the pins or leads since the most damage is done at these
points by ESD. Handle the components by the cap edges or body and the printed circuit
cards by the edges.
Do not handle components or printed circuit cards during transport between work
stations. Components or cards must not be directly handled by anyone not grounded.
Do not use Refrigerant or other chlorinated cleaners at the work area. Use conductive or
anti-static bags and containers for storage and transportation of components or circuit
boards.
HIGH VOLTAGE REMINDER
When you have removed the front panel and/or the rear panel from the instrument and
have the circuit breaker on, there is high voltage (>200VAC/ 300VDC) on some
components. This voltage is there even though the instrument is not running.
REFRIGERATION SYSTEM SAFETY
Working with refrigeration systems requires special equipment and training. If you are
not familiar with the operational theory for refrigeration systems and the special
requirements for performing service on a system, do NOT attempt it. Use the following
precautions for working with refrigeration systems in Beckman instruments.
2. Do NOT inhale refrigerant. It has an intoxicating effect and is harmful to your health.
3. Refrigerant can easily cause "frostbite". Be especially careful with liquid refrigerant.
4. Use caution when replacing a burnt out compressor. The oil in the compressor may
be very caustic or acidic. Use protective gloves.
5. Charge refrigeration systems with gas from the low side only. High side charging
may damage the compressor.
6. Do NOT run a compressor without refrigerant in the system. Compressors are cooled
by the refrigerant and will burn out if there is no cooling.
BRAZING SAFETY PRECAUTIONS
Brazing requires the use of a high-temperature "MAPP Gas" torch and as such many
potential safety hazards exist. Always follow the guidelines below when brazing.
9. Always insure that heated parts have cooled completely before handling them.
ALLEGRA X12 &12R, X-15R
INTRODUCTION 1-1
SCHEMATICS 4-1
PARTS 5-1
APPLICATIONS 7-1
INTRODUCTION
SYSTEM INSTALLATION
SERVICE PROCEDURES
3.3 TROUBLESHOOTING 3 - 15
SCHEMATICS
SCHEMATICS X 15R 4 - 18
SECTION 5
PARTS
APPLICATIONS
SERVICE MEMOS
Time
Set time . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .to 99 hours 59 minutes
or continuous (hold)
Time display
Timed run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . indicates run time remaining
Continuous (hold) or pulse run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . indicates elapsed time
Temperature
Set temperature
Allegra X-12R . . . . . . .. . . . . . . . . . . . . . . . . . . . . . . . . . . . .10 to +40C in 1 increments
Allegra X-12 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .factory set at 20C
Temperature control (after equilibration)
Allegra X-12R . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2C of set temperature*
Allegra X-12. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2C of the 20C set temperature
Temperature display (after equilibration) . . . . . . . . chamber temperature in 1 increments
Ambient temperature range. . . . . . . . . . . . . . . . . . . . . . . . . . . .10 to 35C
Ambient temperature range for optimum operation . . . . . . . . .10 to 25C
Humidity restrictions. . . . . . . . . . . . . . . . . . . . . . . .<75% (noncondensing)
Dimensions
Width . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76.2 cm (30.0 in.)
Depth . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62.2 cm (24.5 in.)
Height, door open. . . . . . . . . . . . . . . . . . . . . . . 96.5 cm (38.0 in.)
Height, door closed . . . . . . . . . . . . . . . . . . . . . 34.3 cm (13.5 in.)
Weight . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 121 kg (267 lb)
Finishes
Control panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . coated polycarbonate
Housing surfaces . . . . . . . . . . . . . . . . . . . . . . . . . . .acrylic baking enamel
Electrical requirements
208-V, 60-Hz instrument . . . . . . . . . . . . . . . . 187229 VAC, 9 A, 60 Hz
200-V, 50/60-Hz instrument . . . . . . . . . . . . 190220 VAC, 10 A, 50/60 Hz
230-V, 50-Hz instrument . . . . . . . . . . . . . . . . . 207253 VAC, 8 A, 50 Hz
Maximum heat dissipation into room under
steady-state conditions . . . . . . . . . . . . . . . . . . . . . . . . 4100Btu/hr (1.2kW)
Noise level 0.91 m (3 ft) in front of centrifuge . . . . . . . . . . . . . . ..68 dBa
Installation category . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .II
Pollution degree. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2
Time
Set time . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .to 99 hours 59 minutes
or continuous (hold)
Time display
Timed run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . indicates run time remaining
Continuous (hold) or pulse run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . indicates elapsed time
Temperature
Set temperature . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10 to +40C in 1 increments
Temperature control (after equilibration) . . . . . . . . . . . . . . . . . . .2C of set temperature*
Dimensions
Width . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76.2 cm (30.0 in.)
Depth . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62.2 cm (24.5 in.)
Height, door closed . . . . . . . . . . . . . . . . . . . . . 34.3 cm (13.5 in.)
Weight . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 128 kg (283lb)
Ventilation clearances (sides and rear) . . . . . . . . . . . . . . . . 7.6 cm (3.0 in.)
Finishes
Control panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . coated polycarbonate
Housing surfaces . . . . . . . . . . . . . . . . . . . . . . . . . . .acrylic baking enamel
Electrical requirements
208-V, 60-Hz instrument . . . . . . . . . . . . . . . . 187229 VAC, 12 A, 60 Hz
200-V, 50/60-Hz instrument . . . . . . . . . . . . 190220 VAC, 12 A, 50/60 Hz
230-V, 50-Hz instrument . . . . . . . . . . . . . . . . . 207253 VAC, 12 A, 50 Hz
Maximum heat dissipation into room under
steady-state conditions . . . . . . . . . . . . . . . . . . . . . . . . . .6800 Btu/hr (2 kW)
Noise level 0.91 m (3 ft) in front of centrifuge . . . . . . . . . . . . . . ..68 dBa
Installation category . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .II
Pollution degree. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2
CENTRIFUGE FUNCTION
The Beckman Coulter Allegra X-12R, X-15R (refrigerated) or Allegra X-12 (constant controlled
temperature) centrifuge (see Figure 1-1) is a benchtop centrifuge that generates centrifugal forces
required for a wide variety of applications. Together with the Beckman Coulter rotors designed
for use in this centrifuge, the centrifuge applications include:
Cell isolation.
Processing large numbers of small-volume samples in multiwell plates for concentrating tissue-
culture cells, cloning and replicate studies, in-vitro cytotoxicity studies, receptor binding, and
genetic engineering experimentation.
The Allegra X-12/R and X-15R are microprocessor-controlled, providing interactive operation.
The instrument design features a brushless asynchronous motor, automatic rotor identification
system, program memory that enables repeated run conditions, temperature control system, and a
choice of acceleration and deceleration rates.
User messages alert the operator to conditions that may need attention.
Available Rotors
CENTRIFUGE CHASSIS
HOUSING
The centrifuge housing is made of sheet steel and structural foam and is finished with acrylic
baking enamel paint. The control panel overlay is made of coated polystyrene copolymer. The
right and left side front panels are made of high-impact polystyrene structural foam coated with
enamel.
DOOR
The aluminum and structural foam door is secured to the housing by solid hinges. A window in
the center allows strobe viewing. When the door is closed, the locking system engages. The door
is locked when a run is in progress and can be opened only when the rotor is stopped and the
LED next to the [Door] key is lit. If there is a power failure, the door lock can be manually
tripped for sample recovery (see Section 4, Troubleshooting).
ROTOR CHAMBER
The aluminum rotor chamber is coated with a chemical resistant epoxy finish. The chamber is
sealed by a labyrinth of four rubber gaskets.
NOTE To avoid chamber icing, refrigeration is off when the door is open.
The centrifuge door must be closed and locked for the refrigeration system to
begin operating.
DRIVE
The asynchronous direct-drive motor is brushless for clean, quiet operation. The resilient
suspension ensures that loads will not be disturbed by vibration, and prevents damage to the drive
shaft if an imbalance occurs during centrifugation. Maximum acceleration and deceleration may
be selected to allow fast processing of samples; alternately, delicate gradients may be preserved
using slower acceleration and deceleration.
SAFETY FEATURES
Saftey features include the following:
PROTECTIVE RING
A structural steel ring surrounding the rotor chamber provides full operator protection.
IMBALANCE DETECTOR
An imbalance detector monitors the rotor during the run, causing automatic shutdown if rotor
loads are severely out of balance. At low speeds, an incorrectly loaded rotor can cause an
imbalance. Rotor instability can also occur if the instrument is moved, or if it is not resting level
on the table (see Section 4, TROUBLESHOOTING).
This safety notice summarizes information basic to the safe operation of the equipment
described in this manual. The international symbol displayed above is a reminder that all
safety instructions should be read and understood before installation, operation,
maintenance, or repair of this centrifuge. When you see the symbol on other pages, pay
special attention to the safety information presented. Observance of safety precautions
will also help to avoid actions that could damage or adversely affect the performance of the
centrifuge.
The centrifuges X-12/R weighs 121 kg ( 267 lb), X-15R weighs 128 kg (283). Do not attempt to
lift or move it without assistance.
Any servicing of this equipment that requires removal of any covers can expose parts that
involve the risk of electric shock or personal injury. Make sure that the power switch is off
and the centrifuge is disconnected from the main power source, and refer such servicing to
qualified personnel.
Do not replace any centrifuge components with parts not specified for use on this instrument.
Electrical Safety
To reduce the risk of electrical shock, this equipment uses a three-wire electrical cord and
plug to connect the centrifuge to earth-ground. To preserve this safety feature:
Make sure that the matching wall outlet receptacle is properly wired and earth-grounded.
Check that the line voltage agrees with the voltage listed on the name-rating plate affixed to
the
centrifuge.
Do not place containers holding liquid on or near the chamber door. If they spill, liquid
may get into the centrifuge and damage electrical or mechanical components.
This centrifuge is not designed for use with materials capable of developing flammable or
explosive vapors. Do not centrifuge such materials (such as chloroform or ethyl alcohol)
in this centrifuge nor handle or store them within the 30-cm (1-ft) area surrounding the
centrifuge.
Mechanical Safety
Use only the rotors and accessories designed for use in this centrifuge.
NEVER attempt to override the door interlock system while the rotor is spinning.
Maintain a 7.6-cm (3-in.) clearance envelope around the centrifuge while it is running.During
operation you should come within the envelope only to adjust instrument controls, if
necessary.
Never bring any flammable substances within the 30-cm (1-ft) area surrounding the
centrifuge.
Never lean on the centrifuge or place items on the centrifuge while it is operating.
Normal operation may involve the use of solutions and test samples that are pathogenic,
toxic, or radioactive. Such materials should not be used in this centrifuge, however, unless
all necessary safety precautions are taken.
Observe all cautionary information printed on the original solution containers prior to
their use.
Handle body fluids with care because they can transmit disease. No known test offers
complete assurance that they are free of micro-organisms. Some of the most virulent
Hepatitis (B and C) and HIV (IV) viruses, atypical mycobacteria, and certain systemic
fungifurther emphasize the need for aerosol protection. Handle other infectious
samples according to good laboratory procedures and methods to prevent spread of
disease. Because spills may generate aerosols, observe proper safety precautions for
aerosol containment. Do not run toxic, pathogenic, or radioactive materials in this
centrifuge without taking appropriate safety precautions. Biosafe containment should
be used when Risk Group II materials (as identified in the World Health Organization
Laboratory Biosafety Manual) are handled; materials of a higher group require more
than one level of protection.
Dispose of all waste solutions according to appropriate environmental health and safety
guidelines.
The Allegra X-12/R, X-15R model centrifuge is customer installed. Installation requirements for
power and location are described below.
Warning
Do not place the centrifuge near areas containing
flammable reagents or combustible fluids. Vapors
from these materials could enter the centrifuge
air system and be ignited by the motor.
SPACE REQUIREMENTS
To reduce the risk of electrical shock, this centrifuge uses 2.5m (8 ft) three-wire electrical cord
(attachedto the power connector at the rear of the instrument) and plug for use in North America
is the NEMA 6-15P; a NEMA 6-15R socket should be available. (Contact your local Beckman
Coulter office for specific information regarding local requirements). To preserve this safety
feature:
Make sure that the matching wall outlet receptacle is properly wired and earth-grounded.
Check that the line voltage agrees with the voltage listed on the name rating plate affixed to
the centrifuge.
If there is any question about voltage, have a qualified service person measure it under load
while the drive is operating.
To ensure safety the centrifuge should be wired to a remote emergency switch (preferably
outside the room where the centrifuge is housed, or adjacent to the exit from that room), in order
to disconnect the instrument from the main power source in case of a malfunction.
Rotor Attachment
It is extremely important for a safe centrifuge operation that the rotor attachment to the drive
shaft is free of play. Otherwise shifts between drive and rotor can develop. Consequences of
this are bent shafts as well as damage to rotors, rotor chamber, etc.
Allegra X-12/R, X-15R centrifuges avoid this by using a conical seat and an additional
expanding connection at the drive shaft. The conical seat is located above the bearing plate,
and the cross-slit end of the shaft with inner cone is the expanding connection. The counterpart
is the boring of the rotor, which is conical at the bottom end.
The rotor is placed onto the drive shaft and the handle is being used to tighten the rotor onto the
shaft. When tightening the spring washers packet is compressed as far as physically possible.
The resulting tension load forces the rotor onto the cone and thus ensures that the bottom end of
the rotor boring is free of play. When tightening the handle further, the cone at the screw
spreads the upper end of the cross-slit shaft apart and thus also presses it against the upper end of
the rotor boring.
Be sure to inform the user of the importance of a secure seating of the rotor and the principle of
the attachment.
Allegra X-12/R, X-15R centrifuges are certified to meet the requirements of the European CE
mark. To meet these requirements, the centrifuge must be secured to the bench using the
anchoring hardware shipped with the instrument. This will prevent the centrifuge from moving
in
the unlikely event of a rotor mishap.Complete instructions for installing the anchoring kit are
packaged with hardware, which is shipped with the centrifuge. The instructions (publication GX-
TB-002A) include a full-size template to be used as a guide for drilling holes in the bench. Refer
to this document for additional installation instructions.
Explain:
Descriptions of instrument
Functions Explain overall functions.
Controls Describe control function of each individual key, program mode.
Indicators Show the different displays and explain audible alerts.
Rotor chamber Instruct to clean and dry chamber after spill and between runs.
Test Run
NOTE: The instrument must be plugged in and the power switch turned to on
position (1) before the door can be opened.
We recommend that you make a test run to ensure that the instrument is in proper operating
condition following shipment.
Show the customer how to setup a simple run. Observe the customer as they setup the run.
Explain:
Troubleshooting
Warning: Unplug the centrifuge or turn off and lock out the main wall circuit
breaker before removing an instrument panel.
Warning: As long as the yellow LED on the Motor driver board is lit, the Bus
Voltage is still present. Wait until LED is off.
Warning: Unplug the centrifuge or turn off and lock out the main wall circuit
breaker before removing an instrument panel.
Warning: As long as the yellow LED on the Motor driver board is lit, the Bus
Voltage is still present. Wait until LED is off.
A. Open lid, TURN POWER SWITCH OFF AND DISCONNECT POWER CORD.
B. Remove four screws on the top housing to open the front panel.
C. Disconnect the drive connector J16 from the Motor Driver board and remove from
the cable holder.
D. Disconnect the ground connector from the motor housing.
E. Disconnect the tach sensor connector J10, Rotor ID connector J8 and Motor Overtemp connector
J11 from the Control Board and remove the cables from the cable holder.
F. Remove the boot from the bowl.
G. Remove two screws on top of the drive that hold the imbalance flag bracket in place.
H. Remove four motor mounting lock-nuts by holding the Philips screws underneath the
suspension plate with a 900 angle Philips screwdriver in place.
I. Remove washers, grommets and screws
J. Pull drive upward, including the white support ring, while feeding the motor harness
through the opening. To ease the removal of the brass cone use a screwdriver to
widen the slit and pull the cone of the shaft.
K. Remove three Phillips screws that attach the Rotor ID sensor plate and retain three
spacers, remove the rotor ID sensor.
L. To reinstall drive, feed harness through the drive opening.
M. Make sure the white ring lines up with the mounting holes in the suspension plate.
N. Carefully set drive down on the suspension plate and ensure the harness is not
pinched.
O. Lightly coat the four new grommets with vacuum grease.
P. Place grommets, washers and nuts on top of drive, make sure screws stay in place and
being held with angled Philips screwdriver while tightening the nuts.
Q. Tighten the four lock-nuts until the specified dimension between the suspension
plate and top of the white ring equals 5/8 inch.
Q. Reinstall Rotor ID and cone.
R. Reconnect all connectors and lock in the cable in the cable holders.
S. Reinstall imbalance bracket.
** Remove silicone sealant from chamber lip and clean boot outer groove of cured
silicone. Apply small bead of GE Silicone #108 to the outer groove of the boot.
T. Reinstall boot.
U. Reinstall front panel.
NOTE: The boot must remain completely seated down around the motor cover to
prevent contact with the rotor.
Warning: Unplug the centrifuge or turn off and lock out the main wall circuit
breaker before removing an instrument panel.
Warning: As long as the yellow LED on the Motor driver board is lit, the Bus Voltage is
still present. Wait until LED is off.
A. Open lid, TURN POWER SWITCH OFF AND DISCONNECT POWER CORD.
B. Remove four screws on the top housing to open the front panel.
C. Disconnect the drive connector J16 from the Motor Driver board and remove the
cable from the cable holder.
D. Disconnect the tach sensor connector J10, Rotor ID connector J8 and motor overtemp connector
J11 from the Control Board.
E. Disconnect the ground wire and remove the cables from the cable holder.
F. Remove the boot from the bowl.
G. Remove four motor mounting nuts.
H. Pull drive upward while feeding the motor harness through the opening.
I. To ease the removal of the brass cone use a screwdriver to widen the slit and pull the
cone of the shaft.
J. Remove three Phillips screws that attach the Rotor ID sensor plate and retain three
spacers, remove rotor ID sensor.
K. To reinstall drive, feed harness through the drive opening.
L. Carefully set the drive down on the suspension plate and ensure that the harness is not
pinched.
M. Insure that the four silicon sleeves are in place.
J. Tighten the four nuts
K. Reinstall Rotor ID sensor and cone.
L. Reconnect all connectors and lock the cable in the cable holders.
** Remove silicone sealant from chamber lip. Apply small bead of GE Silicone #108
to the outer groove of a new boot. Reinstall boot.
M. Reinstall front panel.
NOTE: The boot must remain completely seated down around the motor cover to
prevent contact with the rotor.
Warning: Unplug the centrifuge or turn off and lock out the main wall circuit
breaker before removing an instrument panel.
Warning: As long as the yellow LED on the Motor driver board is lit, the Bus Voltage is
still present. Wait until LED is off.
Warning: Unplug the centrifuge or turn off and lock out the main wall circuit
breaker before removing an instrument panel.
Warning: As long as the yellow LED on the Motor driver board is lit, the Bus
Voltage is still present. Wait until LED is off.
Warning: Unplug the centrifuge or turn off and lock out the main wall circuit
breaker before removing an instrument panel.
Warning: As long as the yellow LED on the Motor driver board is lit, the Bus
Voltage is still present. Wait until LED is off.
Warning: Unplug the centrifuge or turn off and lock out the main wall circuit
breaker before removing an instrument panel.
Warning: As long as the yellow LED on the Motor driver board is lit, the Bus
Voltage is still present. Wait until LED is off.
A. Remove the electrical panel by removing 8 button head screws attached in the back of
the instrument.
B. Remove two screws that hold the brake resistor bracket in place.
C. Replace brake resistor
D. Remove two screws that are attached to the EMI filter and replace filter.
E. Remove two screws that are attached to the receptacle and replace receptacle.
A. Refer to the table below to determine which rotor and loads to be used for calibration.
B. From the centrifuge rotor table, select the appropriate rotor.
C. Install the appropriate test loads per the table below.
D. Press ENTER, 611 ENTER, START.
E. The centrifuge will run the rotor to speed and brake to a stop.
F. Press CE when prompted by the centrifuge.
G. Repeat steps A through F as required.
Warning: Unplug the centrifuge or turn off and lock out the main wall circuit
breaker before removing an instrument panel.
Warning: As long as the yellow LED on the Motor driver board is lit, the Bus
Voltage is still present. Wait until LED is off.
Note:
To reenter calibration value after clearing S1, S2, S7 diagnostics use ENTER 616.
A calibration is necessary when changing the optical flag, optical sensor, motor or motor
mounts.
Sensor Calibration
Note:
An SX4750 rotor needs to be installed before adjusting the voltage below.
Before proceedings with adjustments make sure the flag is free of scratches.
Flag Calibration
J. If voltage is within 2.5 +/- .1Volts DC after moving the rotor in both directions tie
down the bracket.
K. Perform a test run with a balanced rotor load and empty rotor. Monitor the 2.5 V DC
L. If the Voltage is within 2.5+/- .1V between a loaded or empty rotor the calibration is
complete.
M. If it isnt within the tolerance perform steps E to L again.
Warning: Unplug the centrifuge or turn off and lock out the main wall circuit
breaker before removing an instrument panel.
Warning: As long as the yellow LED on the Motor driver board is lit, the Bus
Voltage is still present. Wait until LED is off.
Warning: Refer to the safety notices in the front of this manual for cautionary notes
when working with refrigeration system. Read all brazing safety
notes. Always recover the refrigerant.
B. To precool the rotor and chamber, set temperature to -2 C, speed to 2000 RPM and time
for 30 minutes.
C. After precool, set the temperature to -10C and increase speed to 3750 RPM. Set the time
for 15 minutes.
D. At the instant the time shuts off, record the indicated temperature. The reading should be
-1 C or colder for 60 Hz units and + 1 C or colder for 50 Hz units.
E. When the rotor stops use a temperature meter to measure the actual temperature of the
sample (glycerol) and then record the reading.
B. Connect charging manifold (Access control valves, P/N 964411, simplify connection
to Shrader service valves) and refrigerant supply as shown in Figure 3-1.
C. Before opening the Shrader valves, purge the charging manifold and hoses of air with
R404a refrigerant supply at the low-side and high-side service valves.
D. Precool Chamber to 10 C and install rotor SX 4750. Run the rotor at 3750RPM for
ten minutes and then take pressure readings.
G. If system pressures are low, add refrigerant R404a as a liquid at the low-side until the
required pressure is indicated. Proceed to step 3.2.4 to check for leaks.
H. After removing refrigeration manifold, and access valves, ensure the service valve caps
are replaced and tightened. Insulate the low-side service valve with Presstite insulation
tape.
Figure 3-1
Allegra X-12R
Allegra X-15R
Figure 3-2
LEAK TEST
A. Remove front panel and instrument housing as outlined in the mechanical systems
section.
B. Connect the charging manifold per step 3.2.2.
C. Purge charging manifold and hoses of air before adding refrigerant to the system.
D. If system pressures are low, add refrigerant R404a as a liquid to the low-side with
compressor running.
E. Turn main power off. Allow pressures to equilibrate then test for leaks. (Requires a leak
detector that will detect R404a).
F. Correct all leaks found in the system.
EVACUATION
A. Evacuate the system to 50 microns by using an evacuation manifold and a high capacity
vacuum pump, as shown in figure 3-3. After a vacuum is obtained turn compressor on
for about 2-3 seconds to remove moisture trapped in compressor, turn compressor off and
allow system to achieve 50 microns of vacuum.
B. Close both access service valves at the compressor while the vacuum pump continues to
run. Once the valves are closed, turn off the vacuum pump and remove the evacuation
manifold.
B. Connect charging manifold and R404a refrigerant supply per procedure in step 3.2.3.
Vacuum Monitor
(338866)
Compressor
High Side
Service Valve
Evacuation Manifold
(341676)
Compressor
High Vacuum Low side
Pump Service Valve
FIGURE 3 - 3
C. Disconnect harnesses in brazing area and position them to avoid heat damage.
CAUTION: Do not damage or cut the capillary tube located inside the 1/4 inch tubing.
G. Observe brazing precautions. Unbraze both refrigeration chamber lines .
H. Remove chamber support mounting hardware from bottom side of chassis.
I. Remove the barrier if necessary by removing two screws attached to each barrier bracket.
J. Remove chamber.
K. Re-assemble the chamber, by performing the above steps in reverse order.
DO NOT CUT OR DAMAGE THE CAPILLARY TUBE. Evacuate, charge and
leak test the refrigeration system per step 3.2.3.
NOTE: Ensure chamber gasket and door form a good seal prior to performing
refrigeration tests.
4. The value displayed on the meter should match the value given in the chart below.
10 K at 25 C
Temp. C Res. Temp. C Res. Temp. C Res. Temp. C Res.
-50 441.3K 0 29.49K 20 12.26K 40 5592
-40 239.8K 1 28.15K 21 11.77K 41 5389
-30 135.2K 2 26.89K 22 11.29K 42 5193
-20 78.91K 3 25.69K 23 10.84K 43 5006
-10 47.54K 4 24.55K 24 10.41K 44 4827
-1 30.89K 5 23.46K 25 10.00K 45 4655
6 22.43K 26 9605 46 4480
7 21.45K 27 9227 47 4331
8 20.52K 28 8867 48 4179
9 19.63K 29 8523 49 4033
10 18.79K 30 8194 50 3893
11 17.89K 31 7880 51 3758
12 17.22K 32 7579 52 3629
60 2760 13 16.49K 33 7291 53 3504
70 1990 14 15.79K 34 7016 54 3385
80 1458 15 15.13K 35 6752 55 3270
90 1084 16 14.50K 36 6500 56 3160
100 816.8 17 13.90K 37 6258 57 3054
110 623.5 18 13.33K 38 6026 58 2952
120 481.8 19 12.79K 39 5805 59 2854
3.3 Troubleshooting
System Diagnostics
All System diagnostics are only checked during power up, When a System diagnostic occurs, the user
must call the filed service and cannot clear the diagnostic by pressing the [CE] key.
Power Diagnostics
Momentary power outage will not terminate the run. A Power diagnostic will be given for a power
failure at power glitch or outage. When a Power diagnostic occurs, all program parameters will be
saved and the Power diagnostic will be displayed on the VFD.
Door Diagnostics
Imbalance Diagnostics
The Allegra X-12/R, X-15R centrifuge has a rotor imbalance detector as used in the Allegra 25/64R.
In a situation where an imbalanced rotor is being used an Imbalance diagnostic will be generated and
the instrument will brake the run to a stop. The system reports imbalance diagnostic if the sensor
reading is positive and the door is closed.
Diag. Description Action taken When issued Display
#
I1 Imbalanced rotor. Shutdown with brake I-1 Imbalance, see manual
I2 Bad imbalance detector Shutdown with brake Issued when imbalance I-2 Imbalance Error,
A/D does not change call Service
(Connector not attached)
I3 Imbalance not calibrated Shutdown with brake Issued when Imbalance I-3 Imbalance Error,
calibration value for rotor Call Service
is set to 0. This is the default
value when not calibrated.
I4 Imbalance sensor not calibrated Run will not start Issued when enter/start I4 -Imbalance Error,
pressed call Service
I5 Imbalance flag failure Run will not start Issued when enter/start I5 -Imbalance Error, call
pressed Service
I6 Imbalance flag calibration error Run will not start Issued when enter/start I6 -Imbalance Error, call
pressed Service
Temp Diagnostics
When the software detects the chamber temperature (thermistor) exceeds 50C, or the software reads
the can over temp sensor it will generate an Over temp diagnostic and shutdown the instrument by
coasting. There is no minimum chamber temperature requirement or diagnostic.
Diag # Description Action taken When issued Display
T1 Max temperature exceeded Shutdown no brake, When thermistor reading T-1 Temp, door locks for
door locked for 45 min exceeds 50C 45 minutes, call Service
T2 Chamber overtemp Shutdown no brake, HW sends signal to T-2 Temp, door locks for
lock door 45 minutes control board 45 minutes, call Service
T3 Motor over temp Shutdown, no brake HW sends signal to T-3 Temp, door locks for
control board 45 minutes, see manual
Refrigeration Diagnostics
When the software detects the set temperature is not controlled within a temperature range an error will
be displayed
Diag # Description Action taken When issued Display
C1 Temp exceeds 5C above set No action If reached temp and 4C C-1 Rotor temp exceeds
over set 4C above set
C2 Temp exceeds 8C above set Shutdown no brake, If reached temp and 8C C-2Rotor temp exceeds 8C
above set above set, call Service
C3 Temperature not controlled Shutdown with brake Not trending towards C-3 Temp Error,
set temp call Service
C5 Temperature bad reading Shutdown with brake Software read an C-5 Temp Error,
ambiguous temperature call Service
reading
Drive Diagnostics
Diag # Description Action taken When issued Display
D1 Fault not cleared Shutdown no brake Driver board issues fault; D-1 Drive error,
SW cannot clear call Service
D2 Fault limit exceeded Shutdown no brake More than 2 driver faults D2-Drive Error, call
occurred during run Service
D4 Bus voltage limit exceeded Shutdown, no brake SW detects bus voltage D-4 Drive error, call
over maximum allowed Service
voltage 435 Volt
D5 Driver not powered Shutdown, no brake Drive power lost during D-5 Drive Error, call
run Service
D6 Driver over temperature Shutdown, no brake Signal from driver board D-6 Drive Error, call
indicates an over temp Service
D7 Driver not enabled Shutdown, no brake Driver not enabled after D-7 Drive Error, call
turning On Service
D8 Driver not established Shutdown, no brake Driver not established D-8 Drive Error, call
after turning Off Service
D9 Driver not connected Shutdown, no brake Driver board not D-9 Drive Error, call
connected Service
Speed Diagnostics
Rotor Diagnostics
Diag. # Description Action taken When Issued Display
R1 No index pulse found Shutdown, with brake When running, stopping, R-1 Rotor ID problem,
or pressing ENTER START call Service
R2 No code pulse found Shutdown, with brake When running, stopping, R-2 Rotor ID problem,
or pressing ENTER START call Service
R3 Setup rotor does not match the Shutdown, with brake When running, stopping, R-3 Rotor ID mismatch,
chamber rotor or pressing ENTER START check rotor
R4 No Rotor ID X-12/R only Shutdown with brake When system does not R-4 No Rotor Match,
detect any Magnets see Manual
R5 Unknown Rotor in chamber Shutdown with brake Magnets detected, but R-5 No Rotor Match,
pattern is not one of see Manual
Allegra X-12 R rotors
If facility power fails you will have to restart the run when the power is restored. In the event
of an extended power failure, it may be necessary to trip the door-locking mechanism
manually to remove the rotor and retrieve your sample.
1. Press the POWER switch to OFF and unplug the power cord from the power source.
No displays or LEDs are lit.
2. Make sure that the rotor is not spinning. No sound or vibration comes from the
centrifuge.
! WARNING
Never attempt to override the door interlock system while the rotor is spinning.
3. Locate the release device cap underneath the bottom center of the centrifuge (not
visible from the centrifuge front).
5. Insert the screwdriver through the two cords and pull to release. The latch releases and
the door opens. If the rotor is still spinning, close the door and wait until it stops before
attempting to remove it.
! WARNING
Never attempt to slow or stop the rotor by hand.
Preliminary
Contact the customer prior to making the call. Ask about any current problems, and
decontamination of the instrument if necessary. Order any potentially required parts in
advance.