Enzyme Technology

Course introduction
Sep 05, 2016
Instructor
Won-Heong Lee
Department of Bioenergy Science and Technology
Room 125, College of Agriculture and Life Science Building #4
Tel: 062-530-2046
E-mail: wonhlee@jnu.ac.kr

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Course description
What is enzyme?
Catalyst in biological system
Accelerates reaction rate at ambient conditions
Substrate specific and reaction specific

Objectives of this class

Understanding of fundamentals and broad perspectives on
enzymes that can be utilized to produce a number of value-
added materials

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Lecture description
Mode of class
Lecture: Monday (15:00-16:50) and Wednesday (15:00-15:50)
Lecture in English type A (basically, 100% English)

Grading
Midterm exam + final exam + attendance (40/50/10)
Absolute evaluation will be considered

Examination
Questions for examination will be prepared in English
Many types of questions will be used
Multiple choices, true or false, description, calculation, derivation, etc.

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Textbooks
Principles of Biochemistry (Moran et al., 5th, 2011)
Enzymes in Food Technology (Whitehurs et al., 2nd, 2010)
Bioprocess Engineering: Basic Concepts (Shuler et al., 2nd, 2002)
Molecular Biotechnology: Principle and Applications of
Recombinant DNA (Glick et al., 4th, 2010)

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Lecture Plan
Amino acids and proteins
General introduction of enzymes
Catalytic properties of enzymes
Enzymes in industry (food, chemical and biofuel)
Midterm examination
Microbial production of enzymes
Introduction of protein engineering
Enzyme kinetics
Enzyme bioreactor operation
Final examination
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Question examples
1. Description
There are three chemical reaction mechanisms frequently observed in
enzymatic reactions, nucleophilic substitution, cleavage and oxidation-
reduction. Describe the three chemical mechanisms.

2. Calculation
The following reaction is the initial step of glycolysis in yeast.
ATP + glucose ADP + fructose-6-phosphate
Calculate the G0 (kJ/mol) value for the above reaction with data from the
table of standard free energy changes of some chemical reactions.

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3. Derivation
Consider the reversible product formation reaction in an enzyme-catalyzed
reaction.
k1 k2
S + E ES E + P
k 1 k 2

1 + 2 1 + 2
(where, = , = , = 2 0 , = 1 0 )
1 2
Develop a rate expression for product formation using the quasi-steady-state
approximation and show that

= =

1+ +