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2 2.5 million
animal species
1000 species
A segment of DNA unique to a species
DNA barcode
Hebert et al. 2003; Hebert et al. 2004; dnalc.org
International Barcode of Life
ibol.org
1. DNA barcoding is very technical?
Museum collections
Fieldwork
Sing, Syaripuddin, Wilson. 2016. Malayan Nature Journal
US$0.03 US$0.71
US$0.31 US$5.86
Museum collections
Fieldwork
Wong et al. 2015. Bulletin of Entomological Research
Barcoding workshop/seminar/meeting
UM, Kuala Lumpur, Malaysia
2013
5th BoL Kunming
Mindanao
Barcoded of the
species of Odonata
Wilson et al. 2014. J Asia-Pacific Entomol; Jisming-See et al. 2016. Genome
Clogmia albipunctatus
Brown
Widow
Spider
Rapid biodiversity inventory
ILLUMINA HTS
Brandon-Mong et al. 2015. Bulletin of Entomological Research
Malaysia
Syaripuddin, Sing, Wilson. 2015. Trop Con Sci; Wilson et al. 2015. BDJ
Environmental DNA
Wilson et al. In review. Mitochondrial DNA A
Environmental DNA
Lee, Sing & Wilson. 2015. PLOS ONE; Lee et al. 2016. Genome
Blowfly-derived DNA
Lee, Sing & Wilson. 2015. PLOS ONE
Blowfly-derived DNA
Mammal DNA persists in blowfly
guts for ~72hrs
Blowflies disperse ~1km per day
Our primer set amplifies 205bp
5-COI mini barcode
90% amplification success for
mammal dataset
Lee et al. 2016. Genome
Blowfly-derived DNA
VS.
Lee et al. 2016. Genome
Blowfly-derived DNA
Blowflies
outperform
traditional
methods nets,
scat, traps,
cameras
Bats, rats, cattle,
boar detected
Lee et al. 2016. Genome
Blowfly-derived DNA
Lim et al. In Review. Urban Ecosystems
Trophic relationships
Agriculture
Forest Urban
Lim et al. In Preparation.
Trophic relationships
DNA barcoding is
a team effort
South
China
DNA
Barcoding
Center