Immunomodulation Effect of Meniran (Phyllanthus niruri linn) on

Blood Profile of Broiler Chickens Infected with Enterotoxin of

Antibiotic-Resistant Escherichia coli
Retno Sri Wahjunia,b, Emy Koestanti Sabdoningrumc, Sri Hidanahc, Diyantoroa
Department of Health Science, Faculty of Vocational Education, Universitas Airlangga
a
bDepartment of Basic Medical Science, Faculty of Veterinary Medicine, Universitas Airlangga
cDepartment of Animal Husbandry, Faculty of Veterinary Medicine, Universitas Airlangga

INTRODUCTION

Most of the Indonesian farmers usually use broad-spectrum antibiotics to treat of infectious disease caused by Escherichia coli. Use
of antibiotic might result in deposition of residues in meat, milk, and eggs. Concern over antibiotic residues in animal origin cause
disease and the possible development of resistant strains which cause failure of antibiotic therapy in the clinical situation.1
Meniran (Phyllanthus niruri Linn ) is one of the Indonesian medicinal plants that can be used as an alternative for treatment and
prevention of Escherichia coli infection. Meniran contains two important chemical compounds i.e flavonoid and tannin.
Flavonoid is known to modulate the immune system, while tannin is known as an antiseptic and hemostatic.2
Therefore, based on the above issues, this research was conducted to know the effectiveness of meniran (Phyllanthus niruri Linn)
as an immunomodulator of broiler chicken infected with enterotoxin of antibiotic-resistant Escherichia coli.

RESULTS AND DISCUSSION

METHODS TABLE 1

This experimental research was conducted using thirty broiler chicken

Treatment Average
that was divided into six group. P0+ 13344.320a
P0- 13344.320 ab

Infected with E.coli + no extract of meniran P1 13524.720a

P0+ (as a positive control) P2 13084.840ab
P3 11159.720 b

Without treatment AB 9574.840b

P0- (as a negative control)
Infected with E.coli +
P1 20% concentration of meniran extract
Infected with E.coli +
P2 25% concentration of meniran extract
The result of this research showed that there was a significant
difference of immunomodulation activity in the group with
30% concentration of meniran extract.
The 30% concentration of meniran extract is able to reduce the
number of lymphocytes in the blood.
A lymphocyte is one of the subtypes of white blood cell that has
the ability to produce antibodies in the immune response after
maturation process.

Infected with E.coli +

P3 CONCLUSION
30% concentration of meniran extract
A 30% concentration of meniran extract can decrease the number
of lymphocyte in the blood of broiler chicken infected with
AB Infected with E.coli + Flemequin antibiotics enterotoxin of antibiotics-resistant Escherichia coli.

Extract of meniran was given orally for five days into the 28-day old REFERENCES
broiler chicken that had been previously infected with E.coli. Blood
1 A.E. Bogaard, and E.E. Stobberingh. Antibiotic Usage in Animals:
sample collection and Staining of blood smear was performed to
Impact on Bacterial Resistance and Public Health. Drugs. 58: 589
calculate the percentage of lymphocytes in white blood cell (WBC)
-607. 1999.
differential. The result was analyzed statistically using one way ANOVA
continued with Duncan Multiple Range Test. 2 Mathivanan R, Selvaraj P, Nanjappan K. Feeding of fermented
soybean meal on broiler performance. Int. J Poult
Sci. 2006;5:868872.

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