Poster Session 6

Submission ID: 798

IN-VITRO GLYCEMIC INDEX DETERMINATION OF SOURDOUGH

BREADS PREPARED BY USING SOURDOUGHS COLLECTED FROM
DIFFERENT REGIONS OF TURKEY

1 2 1
EZGI ENOL , MUSTAFA YAMAN , M. ZEKI DURAK

ABSTRACT
Bread is of great importance for diet of Turkish people. Especially white bread is one of the
most consumed foods with its high glycemic index (GI). As known, white sourdough bread or
sourdough bread containing different rate of dietary fibers has lower GI than white bread. The aim of
this study was to collect traditional sourdough samples from different regions of Turkey and determine
the GI of sourdough breads made from these sourdoughs. For this purpose, 5 different traditional
sourdoughs were obtained: 4 different homemade white sourdoughs (Ouzlu (O), Kastamonu (K),
Lleburgaz (L), Afyon/ Bolvadin 2 (B2)) and 1 commercial whole wheat sourdough, Safranbolu
Yldz Frn (SYF). Sourdough breads were prepared using the dough of these samples with their own
flour and long timed-cold fermentation (18h). Then GI values of the prepared sourdough breads were
evaluated by using an in vitro method based on Goniassay calculating the GI by measuring rate of
starch digestion. A similar glycemic response was observed for sourdough breads except whole wheat
sourdough bread. O, K, L, B2, SYF had GI of 50.050.3, 51.81.65, 500.5, 510.5 and 46.5 0.5,
respectively. Compared to the control (White bread, GI: 70), the GI of sourdough breads was found
low (<55). There was no significant difference among the GI values of white sourdough breads
collected from different regions. The difference of SYF was thought to stem from dieatary fiber. There
was a negative correlation between the GI value and sourdough - dietary fiber. According the results,
the ability of breads to lower GI was affected by sourdough, dieatry fiber and long timed-cold
fermentation.

KEYWORDS
in vitro glycemic index, sourdough, dietary fiber, fermentation temperature

Corresponding Author: M. ZEK DURAK, mzdurak@yildiz.edu.tr

1
YILDIZ TEKNK NVERSTES
2
STANBU ZAM NVERSTES

891
 Poster Session 6
Submission ID: 799

CHEMICAL COMPONENTS AND ANTIMICROBIAL ACTIVITIES OF

NEST MATERIALS DOLICHOVESPULA SAXONICA

1 2 1
MER ERTRK , CEREN YAVUZ , ZEYNEP KOLREN

ABSTRACT
Social wasp nests serve as a place for rearing brood and the centre for their nesting activities
[1,2]. Wasps collect plants, woody fibers, mud, which they masticate and mix with oral secretion to
construct the nest with a variety of architectural design [1]. This salivary secretion is also used to
physically maintain their nests. In the present study, were determinated the antimicrobial effects of bee
nest samples obtained from East Black Sea Region. The nest of D. saxonica collected in Trabzon
during July-August 2015 Region of East Black Sea. Larvae, pupae and eggs were removed from the
nest. Small fragments were cut from the comb for observation. The nests were stored in the
Entomology Laboratory at Biology Department of Ordu University. The antimicrobial effects of bee
nest sample was determined with disc diffusion method. The antimicrobial activity of bee nest samples
was studied using ten microorganisms. Three gram-positive: Staphylococcus aureus ATCC25923,
Micrococcus luteus B1018, Basillus subtillis B209, and five gram negative: Proteus vulgaris B123,
Klebsiella pneumonia ATCC13883, Pseudomonas aeruginosa ATCC27853, Streptomyces murinus
ISP 5091, Yersina enterocolitica ATCC27729, and two fungus ATCC10231, Aspergillus niger
ATCC 9642. The antibacterial activity of diluted with ethanol five fraction nest was assayed in vitro
by agar disc diffusion method against 8 bacterial and 2 fungi species. The five dilute nest ethanol
extract showed Antibacterial antifungal activity. On the other hand, ethanol extracts of almost all the
nest exhibited antibacterial antifungal activity towards one or another bacterium against to all of
microorganism used in this study. The maximum antibacterial antifungal activity was shown by
diluted 25 L (2.5 mg) of nest extracts 20 L (2 mg), followed by and 15 L (1.5mg), respectively. In
conclusion, beeswax nest extracts possess a broad spectrum of activity against a panel of bacteria
responsible for the most common bacterial diseases. Key words: Dolichovespula saxonica,
antimicrobial activity

KEYWORDS
Dolichovespula saxonica, antimicrobial activity

Corresponding Author: CEREN YAVUZ, ceren.yavuz@amasya.edu.tr

1
ORDU NVERSTES
2
AMASYA NVERSTES

892
 Poster Session 6
Submission ID: 800

FATTY ACID COMPOSITION OF SOME EUPHORBIA SPECIES BY

USING GC/MS

1 2 3 4 5
SMAIL YENER , ABDULSELAM ERTA , SERKAN YIITKAN , MURAT YOLCU , HILAL SARUHAN FIDAN , ESRA
2 6 7 8 9
YARI , YETER YEIL , MEHMET ZTRK , HAMDI TEMEL , UFUK KOLAK

ABSTRACT
Euphorbia genus have been investigated for a long time in view of different specialities, like
more energy content as alternative source of hydrocarbons, laticifers , phytochemicals and systematics.
Mediterranean Euphorbia species have been the object of various studies and they have been proposed
as potential renewable sources of unsaturated and uncommon fatty acids. Euphorbia genus patterns has
been found to include chemotaxonomically important myrsinane diterpenoids and cycloartane
triterpenoids. The aim of this study was to determine the fatty acid composition of petroleum ether
extracts of Euphorbia aleppica, E. eriophora, E. grisophylla, E. seguiriana subsp. seguiriana, E.
craspedia, E. denticulata and E. fistulosa. E. aleppica, E. eriophora, E. grisophylla, E. seguiriana subsp.
seguiriana, E. craspedia, E. denticulata and E. fistulosa species were collected from Turkey
(Diyarbakr, Diyarbakr, Van, Diyarbakr, Mardin, Kayseri and Diyarbakr, respectively) and
identified by Mehmet Frat (Yznc Yl University, Faculty of Education, Department of Biology).
Powdered form of the whole plant material was weighed (100 g) and macerated three times with
petroleum ether (250 mL each) at 25 C for 24 hours. Esterification of the petroleum ether extract and
GC/MS procedures were applied as described by Ertas et al . Thermo Scientific Polaris Q GC-MS/MS
instrument was used. The major fatty acid components were identified as 17-tetratriactonen (31.59%)
for E. aleppica, palmitic acid (43.83%) for E. eriophora, hexatriasontan (52.32%) for E. grisophylla,
17-tetratriactonen (19.86%) for E. seguiriana subsp. seguiriana linoleic acid (40.52%) for E. craspedia,
17-tetratriactonen (64.75%) for E. denticulata and hexatriasontan (38.13%) for E. fistulosa.

KEYWORDS
Euphorbia, Fatty acid, GC-MS

Corresponding Author: SERKAN YTKAN, syigitkan@hotmail.com

1
DCLE UNVERSTY, FACULTY OF PHARMACY, DEPARTMENT OF ANALYTCAL CHEMSTRY, DYARBAKR
2
DCLE UNVERSTY, FACULTY OF PHARMACY, DEPARTMENT OF PHARMACOGNOSY, DYARBAKIR
3
DCLE UNVERSTY, FACULTY OF PHARMACY, DEPARTMENT OF PHARMACEUTCAL BOTANY,
DYARBAKIR
4
DCLE UNVERSTY, FACULTY OF PHARMACY DEPARTMENT OF PHARMACEUTCAL TOXCOLOGY
5
DCLE UNVERSTY, FACULTY OF PHARMACY, DEPARTMENT OF BOCHEMSTRY
6
ISTANBUL UNVERSTY, FACULTY OF PHARMACY, DEPARTMENT OF PHARMACEUTCAL BOTANY
7
MUGLA SITKI KOCMAN UNVERSTY, FACULTY OF SCENCE, DEPARTMENT OF CHEMSTRY
8
DCLE UNVERSTY, FACULTY OF PHARMACY DEPARTMENT OF PHARMACEUTCAL CHEMSTRY
9
ISTANBUL UNVERSTY, FACULTY OF PHARMACY, DEPARTMENT OF GENERAL AND ANALYTCAL
CHEMSTRY, ISTANBUL

893
 Poster Session 6
Submission ID: 801

DETERMINATION OF BIOACTIVE COMPONENTS OF THYMUS

PRAECOX OPIZ. SSP. GROSSHEIMII PLANT BY USING ON-LINE
HPLC-FRAP METHOD

1 2 2
NESIBE ARSLAN BURNAZ , MURAT KK , KAMIL COKUNELEB

ABSTRACT
Thymus praecox OPIZ ssp. grossheimii (Ronniger) Jalas, which is an aromatic plant with its
own odor, is used for therapeutic purposes. It is a thyme species and is known as the "yayla ay" in
the Surmene, county of Trabzon, in Turkey. It is consumed as herbal tea because of its appetizing,
digestion facilitating and nervous relaxing effects. It also has antimicrobial and antioxidant effects and
is uses in cases of colds. In this study, FRAP (Fe (III) reduction antioxidant power) assays were
performed by spectrophotometric (off-line) and on-line HPLC methods in yayla ay extracts prepared
in six different solvents (water, methanol, ethanol, isopropanol, butanol and acetonitrile). By the
developed post-column HPLC-FRAP method, separation and antioxidant activity determination were
performed simultaneously. In the course of method development with HPLC, fifteen phenolic acid
standards were used (gallic acid, protocatechuic acid, protocatechuic aldehyde, p-OH benzoic acid,
chlorogenic acid, vanillic acid, caffeic acid, syringic acid, vanillin, p-coumaric acid, syringic aldehyde,
benzoic acid, ferulic acid, sinapic acid and rosmarinic acid). When chromatograms of yayla ay
extracts in different solvents were examined, the most phenolic acids (protocatechuic acid,
protocatechualdehyde, p-OH benzoic acid, vanillic acid, caffeic acid, syringic acid, ferulic acid) were
detected in the aqueous extract of yayla ay. In addition, when the spectrophotometric (off-line) iron
(III) reduction potential values of 1:10 diluted samples of yayla ay extracts were compared in M
FRAP and M TEAC, especially the iron (III) reduction antioxidant power value (1501 M FRAP,
590 M TEAC) of aqueous extract was found significantly higher than the others. The fact that this
plant is consumed as a tea brewed with water among the people and shows highest activity in aqueous
extract makes consumption of yayla ay become important (*). *This work reflects a part of the
doctoral thesis prepared in Karadeniz Technical University, Institute of Science, Department of
Chemistry and was supported by Scientific Research Project of KTU. Project No. 666 (2008-
111.02.5).

KEYWORDS
on-line HPLC, spectrophotometric, FRAP, different solvents, antioxidant

Corresponding Author: NESBE ARSLAN BURNAZ, nsbburnaz@gmail.com

1
GMHANE NVERSTES
2
KARADENZ TEKNK NVERSTES

894
 Poster Session 6
Submission ID: 802

DETERMINATION OF ANTIHYPERGLYCEMIC AND

ANTIOXIDATIVE EFFECTS OF PRUNUS LAUROCERASUS
EXTRACT ON STREPTOZOTOCIN-INDUCED EXPERIMENTAL
TYPE I DIABETIC RATS

1 2 3
HAMIT USLU , GZDE ATLA , MUSA KARAMAN

ABSTRACT
Diabetes mellitus is a metabolic disorder that is diagnosed with hyperglycemia resulting from
defects in insulin secretion, insulin action, or both. The chronic hyperglycemia can adversely effect
various organs like as eyes, kidneys, liver, nerves, heart, and blood vessels. The existing drugs are not
treating diabetes only in the prevention of complications. Nowadays researchers are directed to find
new sources due to the lack of certain treatment of diabetes. For this reason herbal sources have begun
to be preferred because of the less or no side effects. The extract used in this study was obtained from
Prunus laurocerasus leafs. Prunus laurocerasus is a member of the Rosaceae family which is known
wild cherry or chery laurel. This plant is mostly spread on during the coast of the Black Sea region of
Turkey and is locally called Taflan or Karayemi. This species is also well known as a traditional
medicine in northern side of Anatolia; the leave extract is used in the therapy of coughs, hemorrhoids,
eczemas, asthma, digestive system complaints as well as in the treatment of stomach ulcers. In
northern Anatolia, the fruits and seeds of Cherry laurel are used against Diabetes mellitus and its
complications widely on the grounds that useful in among to local people. In this study, we aimed to
determine effect of administering oral Cherry laurel leaf extract and subcutaneous insulin for 28 days
on fasting blood glucose and glycated hemoglobin (HbA1c) levels and oxidative stress. It was
determined that Prunus laurocerasus plant has antioxidant properties in vitro total phenolic component,
nitric oxide scavenging and iron reduction tests. A total of 60 Spraque-Dawley rats, aged 2 months,
divided into 6 groups including 10 animals, were used. Groups were consist of normoglycemic control
group, diabetic control group, 500 mg/kg (PL500), 1000 mg/kg (PL1000), 1500 mg/kg (PL1500) leaf
extract administrating groups and insulin group (2IU). The rats were defined as type I diabetic if the
fasting blood glucose levels were higher than 200 mg/dL after 72 hours of Streptozotocin
administration. Blood glucose levels in insulin-treated group significantly decreased from day 7th
(p<0.05). At the end of study blood glucose levels of PL500, PL1000 and PL1500 groups lower than
diabetic control group, though this reduction was not significant (p>0.05). HbA1c levels significantly
increased (p<0.05) as serum insulin levels decreased (p<0.001) in the diabetic control group. However
no significant change in both insulin and HbA1c levels in the experimental group (p>0.05).
Thiobarbituric acid reactive substances (TBARS) levels increased in both the liver and kidney tissues
(p<0.05, p<0.001 respectively), while catalase (CAT) (p0.001) and superoxide dismutase (SOD)
(p<0.001, p<0.05 respectively) levels decrease significantly in the diabetic control group compared to

Corresponding Author: HAMT USLU, hamit_uslu@hotmail.com

1
DEPARTMENT OF HEALTH CARE SERVCES, ATATRK VOCATONAL SCHOOL OF HEALTH SERVCES,
UNVERSTY OF KAFKAS
2
DEPARTMENT OF PHSOLOGY, FACULTY OF VETERNARY MEDCNE, UNVERSTY OF KAFKAS
3
DEPARTMENT OF PATHOLOGY, FACULTY OF VETERNARY MEDCNE, UNVERSTY OF BALIKESR

895
normoglycemic control group. Despite significant reduction of TBARS levels in kidney tissue at the
end of substances use (p0.001), this reduction was observed only in the liver of the insulin group
(p<0.05).SOD enzyme levels of the PL500, PL1000, PL1500, and of the insulin groups were
significantly higher than the liver (p<0.01) and kidney (p0.001) tissues of the diabetic control group.
CAT enzyme levels in liver (p<0.05) and kidney (p<0.01) tissues were increased by insulin
administration, but the changes in other groups were not significant (p>0.05). In conclusion, Prunus
laurocerasus has not been a significant effect on hyperglycemia, HbA1c and insulin levels in diabetes,
but it has been determined that to be effective in reducing oxidative damage by decreasing TBARS
levels and increasing SOD levels.

KEYWORDS
Prunus laurocerasus, Type I Diabetes mellitus, Oxidative stress, Antioxidant

896
 Poster Session 6
Submission ID: 803

LAVENDER ESSENTIAL OIL IN CLINICAL PRACTICE

1 1 2
ENES BULUT , DLEK LNGR , CEYDA UZUN AHN

ABSTRACT
Patients take health care due to their health problems. In health care process, people get
medical treatment and/or surgical treatment. Because they think that they will have high level of pain
and they will die due to operation, especially in surgical treatment people are anxious. Also, because
there are invasive approaches, enfection risk is available. These problems increase the hospitalization
time and healt care costs. Also they effect negatively the quality of health of people. To eliminate
these possible situations, drugs have different effects are used. But using many drugs cause liver
dysfunction. To hinder this, today complementary therapies are used commonly. Aromatherapy is one
of these therapies. In this therapy, essential oils extracted from aromatic plants are used. These oils can
be applied by inhalation, topical or massage. Lavender essential oil is well known. This essential oil
has different properties. Some of these properties are antiseptic, antifungal, sedative, anxiolytic and
analgesic activity. In the literature, it is seen that lavender essential oil is used for different aims and it
has positive effects. Soltani et al. (2013) found that aromatherapy with lavender essential oil was
effective to decrease the number of required analgesics after tonsillectomy. Ghods et al. (2015) stated
that topical application of lavender essential oil decreased intensity of pain related to dialysis needles.
In an other study conducted with patients undergoing coronary artery bypass graft surgery, Bikmoradi
et al. (2015) found that lavender aromatherapy decreased the systolic blood pressure. Also Vakilian et
al. (2011) stated that lavender was superior to povidone-iodine for episiotomy wound care. As seen in
the studies, lavender essential oil is important in managing the symtoms which can be seen in health
care. By generalizing this therapy, a qualified care can be provided to patients. For this, more scientific
meetings should be organized and people should be informed.

KEYWORDS
aromatherapy, clinical, essential oil, lavender

Corresponding Author: ENES BULUT, ebulut61ts@hotmail.com

1
KARADENZ TEKNK NVERSTES SALIK BLMLER FAKLTES
2
RECEP TAYYP ERDOAN NVERSTES

897
 Poster Session 6
Submission ID: 804

NORHARMANE PRODUCTION OF ANABAENA ORYZAE UNDER

DIFFERENT TEMPERATURE AND LIGHT CONDITIONS

1 1
TNAY KARAN , RAMAZAN ERENLER

ABSTRACT
Cyanobacteria are photosynthetic prokaryotes found in all around the world including the
extreme condition. Cyanobacteria have been accepted as one of the most promising groups of
organisms having biologically active natural products. Herein, Anabaena oryzae was collected from
freshwater and was isolated under inverted microscope. Identification was carried out by
morphologically. The isolated Anabaena oryzae was cultivated in BG11 nutrient medium. The
different temperature and light conditions were executed at 15 C and 35 C at the irradiation of 1896
lux and 4300 lux. The norharmane production was determined by HPLC using the C18 120 A reverse
phase column. Based on the results, the amount of norharmane at 15 C and 1896 lux, 15 C and 4300
lux, 35 C and 1896 lux, 35 C and 4300 lux were 0.231, 0.268, 2.741, 1.016 g/g respectively.

KEYWORDS
Anabaena oryzae, norharmane, HPLC, light, temperature

Corresponding Author: TNAY KARAN, biyo_tunay@hotmail.com

1
GAZOSMANPAA NVERSTES

898
 Poster Session 6
Submission ID: 805

EVALUATION OF PULPS OF THE FRAGRANT JUNIPER

(JUNIPERUS FOETIDISSIMA) CONES USED FOR PRODUCTION IN
THE ISPARTA REGION

1 2 1 2
HASAN ASLANCAN , SMET DOKUR , RAFET SARIBA , NAZLI ZG

ABSTRACT
Total forest area in our country is 21,678,134 ha and it is constitutes 27.6 % of our total land
size. Approximately 4.29 % (958.423 hectares) of this area constitutes the junipers that have the
potential to use as a non-wood forest product. In our country, especially Eirdir Forest Nursery, Forest
Nursery Directorates collect an average of 85 tons of cones every year and about 5 million fragrant
juniper (Juniperus foetidissima) saplings produced from these cones. During this production, about 20
tons of seeded cone emerged and this pulp completely discarded. In this study, the evaluation
possibilities of the Fragrant Juniper (Juniperus foetidissima) pulp as a non-wood forest product
investigated. In the study, it was determined that the fragrant Juniper (Juniperus foetidissima) cone
pulp contained about 2% volatile oil. It found that about 400 liters of juniper volatile oil could
obtained annually when about 20 tons of pulp produced.

KEYWORDS
Non-wood forest product , Juniperus foetidissima, essential oil, essential oil component.

Corresponding Author: HASAN ASLANCAN, hasan.aslancan@tarim.gov.tr

1
MEYVECLK ARATIRMA ENSTTS MDRL ERDR / ISPARTA
2
ERDR ORMAN FDANLII MDRL - ISPARTA

899
 Poster Session 6
Submission ID: 806

ISOLATION OF AXILLARIN FROM TANACETUM ALYSSIFOLIUM

AND DETERMINATION OF ANTICANSEROGEN AND
ANTIOXIDANT PROPERTIES

1 1 1 2 2
SAMED IMEK , EKREM KKSAL , MUSTAFA ATIR , ALI RIZA TFEKI , BRAHIM DEMIRTA

D
ABSTRACT

TE
The aim of this study was to isolate the available secondary metabolites in Tanacetum
alyssifolium plants by column chromatography and determine their structures with spectroscopic
methods. It was also aimed to examine the antioxidant and anticanserogen properties of Tanacetum
alyssifolium. For this purpose, Tanacetum alyssifolium plants was collected from the foothills of

EN
Munzur Mountains in Erzincan province and dried at room temperature. The upper part of ground
plant was extracted with ethyl acetate/ butanol solvent system. The ethyl acetate extract was subjected
to the procedure of column chromatography. The structure of the isolated compound with column
chromatography method was elucidated by spectroscopic techniques (1D-NMR, 2D-NMR, HPLC-
TOF). The isolated compound was determined as 2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-3,6-
ES
dimethoxy-4H-chromene-4-on (axillarin). The antioxidant capacity of the isolated compound was
evaluated with CUPRAC method and DPPH radical scavenging activity tests, respectively. Trolox was
used as standard antioxidant. In terms of the iron (Fe+3) reduce ability, axillarin compound was found
to have more reduction potential than standard trolox and when compared the cupper (Cu+2) reduce
ability, axillarin compound displayed less reduction potential. However, axillarin and trolox showed
PR

close results in terms of DPPH radical scavenging capacities. For anticanserogen tests, 3 different
concentrations of axillarin compound were prepared and applied to HeLa cells in order to determine
the concentration at which the maximal activity was shown. The highest anticanserogen activity was
observed at 50 g/mL and reached at 43 hours.
T

KEYWORDS
O

Tanacetum alyssifolium, isolation anticancerogen activity, antioxidant activity

N

Corresponding Author: EKREM KKSAL, koksalekrem@gmail.com

1
ERZNCAN NVERSTES
2
ANKIRI KARATEKN NVERSTES

900
 Poster Session 6
Submission ID: 807

THE EFFECTS OF TARAXACUM OFFICINALE EXTRACT ON

HYPERLIPIDEMIA, OXIDATIVE STRESS AND VASCULAR
CONTRACTIONRELAXATION RESPONSES IN EXPERIMENTAL
TYPE II DIABETES

1 2 3 2 4 2
HAMIT USLU , GZDE ATLA , EMIN ENGL , VOLKAN GELEN , DINER ERDA , MUSTAFA MAKAV

ABSTRACT
Diabetes mellitus is a very important health problem increasing day by day in the world. This
disease negatively affects of the entire body, especially the cardiovascular system. Nowadays, there is
no drug that treats diabetes precisely; for this reason scientists to search for new sources of may be
effective on diabetes and its complications. Taraxacum officinale, a member of the Asteraceae family,
its expressed that to be effective in the treatment of disorders such as upper respiratory tract infections,
jaundice, anemia, fever, eye problems, gastrointestinal problems, eczema in traditional medicine.
There are also expressions that Taraxacum officinale may have an impact on the complications of
diabetes. In this study, we aimed to determine the effects of administering oral Taraxacum officinale
extract and metformin for 30 days on the hyperlipidemia, oxidative stress and vascular contraction
relaxation responses in type II diabetic rats. Taraxacum officinale plant was collected from Trabzon
province and then extracted with ethanol using soxhlet method. It was determined that Taraxacum
officinale plant has antioxidant properties in vitro total phenolic component, nitric oxide scavenging
and iron reduction tests. In this study a total of 50 Spraque Dawley male rats, aged 3 months, divided
into 5 groups including 10 animals, were used. Groups were consist of normoglycemic control (NC),
extract (E) (50 mg/kg), diabetic control (DC), diabetic + extract (D + E) (50 mg/kg) and diabetic +
metformin (D + M) (1000 mg/kg). Rats with fasting blood glucose levels of 200 mg/dL 7 days after
nicotinamide + streptozotocin injection were defined as type II diabetics. Serum HDL, LDL and
triglyceride levels were determined by commercial ELISA kits. MDA, GSH and GPx analyzes were
performed spectrophotometrically in homogenizates obtained from heart and aortic tissues.
Furthermore, thoracic aorta tissues were used for take values of phenylephrine (PE 10-910-5 M)
induced contraction acetylcholine (ACh 10-810-5 M) induced relaxation in the isolated organ bath.
The triglyceride level decreased significantly in the D+E (p<0.05) and D+M (p<0.001) groups
compared to the DC group. LDL cholesterol level increased, while HDL cholesterol level decreased
significantly in the DC group (p<0.01). Although there were some positive changes in HDL and LDL
cholesterol levels of D+E and D+M groups, this changes was not significant (p>0.05). The MDA level
of aortic tissue was significantly elevated in the DC group (p<0.01), but this result did not change with
substance applications (p>0.05). There was no change in the MDA levels of heart tissue among groups

Corresponding Author: HAMT USLU, hamit_uslu@hotmail.com

1
DEPARTMENT OF HEALTH CARE SERVCES, ATATRK VOCATONAL SCHOOL OF HEALTH SERVCES,
UNVERSTY OF KAFKAS
2
DEPARTMENT OF PHSOLOGY, FACULTY OF VETERNARY MEDCNE, UNVERSTY OF KAFKAS
3
DEPARTMENT OF PHSOLOGY, FACULTY OF VETERNARY MEDCNE, UNVERSTY OF ATATRK
4
DEPARTMENT OF MEDCAL SERVCES AND TECHNQUES, ATATURK HEALTH VOCATONAL SCHOOL,
UNVERSTY OF KAFKAS

901
(p>0.05). GSH levels were significantly elevated in the D+E (p<0.01, p<0.05 respectively) and D+M
(p<0.01) groups in both the heart and the aortic tissues compared to the DC group. Although GPx
level of the DC group was significantly reduced (p<0.05) in aorta there was no change in the diabetic
trial groups (p>0.05). GPx level did not change in heart tissue of the DC group (p>0.05), but it was
significantly increased in the D+M group (p<0.001). In the thoracic aorta induced with ACh (10-5 M)
values of relaxation were significantly lower in the DC group compared to other groups (p<0.05). In
the thoracic aorta induced with PE (10-7, 10-6 and 10-5 M) values of concentrations were significantly
high in the NC group compared to other groups (p<0.05, p<0.01, p<0.01 respectively). As a result, it
was determined that Taraxacum officinale extract has significant positive effects on the lipid
metabolism, antioxidant system and vessels contractionrelaxation mechanisms distrupted in type II
diabetes.

KEYWORDS
Taraxacum officinale, Type II diabetes, Hyperlipidemia, Oxidative stress, Vascular
contractionrelaxation

902
 Poster Session 6
Submission ID: 808

A DIFFERENT USE AREA OF AROMATIC PLANTS: LIQUID SMOKE

FOR MEAT PRODUCTS

1
CEM OKAN ZER

D
ABSTRACT
Researchers and industry in many different areas have been an increased interest in medicinal

TE
and aromatic plants in recent years. However, aromatic plants have been used in preservation of meat
products by smoking process for centuries. The smoking process of meat products is one of the most
ancient and important methods. Smoking process contribute flavor and color of meat products with
aromatic compounds and additionally show bacteriostatic and antioxidant effect. However, from a
human healthy point of view, smoking with gases is not very favorable as it generates several
EN
polyaromatic hydrocarbons that are carcinogenic compounds. Therefore, liquid smoke has been used
as an alternative method to gas smoking in recent years. The organoleptic smoke properties depend on
the smoke composition and therefore depend on the source from which it is produced. Some aromatic
plants such as daphne, juniper, thyme and linden have used in liquid smoke. These aromatic plants
ES
provide suitable gas composition that contains some phenols, carbonyls, alcohols and organic acids for
meat products. In this review study, properties and usage areas of liquid smoke produced from
aromatic plant for meat industry are explained.
PR

KEYWORDS
Aromatic plants, smoke process, meat products
T
O
N

Corresponding Author: CEM OKAN ZER, cemokanozer@nevsehir.edu.tr

1
NEVEHR HACI BEKTA VEL UNVERSTY

903
 Poster Session 6
Submission ID: 809

IMPORTANT FUNGAL DISEASES IN CUMIN PRODUCTION AND

SUGGESTIONS FOR THE DISEASE MANAGEMENT

1 1 2 2
GKSEL ZER , HSEYIN KABAKCI , GLSM PALACIOLU , HARUN BAYRAKTAR

ABSTRACT
Cumin is an important spice plant that is widely grown in the Central Anatolia Region of
Turkey and commonly used in cooking and also in traditional medicine because of its essential oils.
Cumin production is seriously affected by some seed-borne and soil-borne plant pathogens causing
significant crop losses. Different fungi species that cause diseases were determined previously in
cumin production. Fusarium wilting caused by Fusarium oxysporum f.sp. cumini and Alternaria blight
caused by Alternaria burnsii have been identified as the most important diseases limiting the
production of cumin in Turkey. In the field observations, Alternaria blight was determined as the most
widespread in the periods after flowering and the losses could be up to 100% in some areas. It has
been observed that F. oxysporum f.sp. cumini restricts seed germination, destroys the root systems and
causes typical signs of wilting. In this study, the prevalence of the pathogens, favorable environmental
conditions required for the disease epidemic and genetic structures of the pathogens were assessed.
The necessary elements to be considered in the breeding studies and integrated diseases management
methods to prevent possible losses have been discussed.

KEYWORDS
Cumin, Alternaria blight, Fusarium wilt, Disease management

Corresponding Author: GKSEL ZER, ozer_g@ibu.edu.tr

1
ABANT ZZET BAYSAL NVERSTES, ZRAAT VE DOA BLMLER FAKLTES, BTK KORUMA
BLM
2
ANKARA NVERSTES, ZRAAT FAKLTES, BTK KORUMA BLM

904
 Poster Session 6
Submission ID: 810

ANTIOXIDANT, ANTIMICROBIAL ACTIVITIES, AND PHENOLIC

COMPOUNDS OF LYCOPUS EUROPAEUS DETERMINED BY LC/MS-
MS

1 2 2 3 1 4
ABDLMELIK ARAS , EKREM KKSAL , HATICE TOHMA , MER KILI , YUSUF ALAN , LHAMI GLIN , ERCAN
1
BURSAL

ABSTRACT
Evaluation of biological activities of medicinal plants is important, because they are the
largest natural source of pharmaceuticals. In the present study, we assessed antioxidant, antimicrobial
activities, and phenolic composition of Lycopus europaeus, (L. europaeus) which is widely known and
used in medicinal treatments in different part of the globe. For Antioxidant activity of the sample,
CUPRAC, FRAP and DPPH assays were utilized. For Antimicrobial activity properties, disc diffusion
method was used against three Gram positive, four Gram-negative microbial species and three fungi
species. Phenolic composition of L. europaeus was evaluated by LC/MS/MS technique. The result
showed that water and ethanol extract of L. europaeus exhibited moderate metal reducing and DPPH
radical scavenging activities compared to standard antioxidants (BHA, BHT and ascorbic acid). The
sample showed the strongest antibacterial activity against the B. megaterium, K. pneumoniae and E.
aerogenes, weak antibacterial activity against P. aeroginosa, S. aureus and E. coli however no
antifungal activity. The HPLC-MS/MS analysis has revealed that Rosmarinic and Kaempferol are the
most abundant phenolics among the studied 27 compounds. The findings of this study have could be
useful for the preparation of high-value medicines and functional ingredients for foods.

KEYWORDS
Lycopus europaeus, CUPRAC, FRAP, DPPH, LC/MS/MS and phenolics

Corresponding Author: ABDLMELK ARAS, abadi.49@hotmail.com

1
MU ALPARSLAN NVERSTES
2
ERZNCAN NVERSTES
3
BNGL NVERSTES
4
ATATRK NVERSTES

905
 Poster Session 6
Submission ID: 811

TOTAL PHENOLIC CONTENT AND ANTIMICROBIAL ACTIVITY OF

METHANOL EXTRACT OF GERANIUM MACRORRHIZUM L.
(GERANIACEAE) NATURALLY FOUND IN TURKEY

1 1 1 1 1
S. ELIF KORCAN , IBRAHIM BULDUK , YASEMIN S. KARAFAKIOLU , MERAL ZTRK , HAVA KLEMEK ,
1
AHMET KAHRAMAN

ABSTRACT
The genus Geranium L. is taxonomically classified within the family Geraniaceae and
comprises about 400 species distributed in temperate regions and tropical mountains all over the
world. Turkish Geranium includes 40 species, 9 of which are endemic. G. macrorrhizum L. is mainly
distributed in temperate zones of Europe, but in Turkey it is only found on the Kapda Peninsula
(Erdek, Balkesir Province). This species is a perennial herbaceous plant with a stout, long, cylindrical
and horizontal rhizome, orbicular leaves and erect stems with entire and magenta petals. Geranium has
been used in folk medicine for the treatment of various ailments due to its therapeutic characteristics,
such as anticarcinogenic, antipyretic and antiseptic in wounds. However, total phenolic content and
antimicrobial activity of many species of this genus have been virtually unexplored. In this study, total
phenolic content and antimicrobial activity of methanol extracts of G. macrorrhizum in Turkey were
comprehensively investigated for the first time. The level of total phenols in methanol extracts was
determined by using FolinCiocalteu reagent and external calibration with gallic acid. The absorbance
was measured at 760 nm using a spectrophotometer. The concentration of the total phenolics was
calculated as mg of gallic acid equivalent by using an equation obtained from gallic acid calibration
curve. The total amount of phenolic substance in the extracts was 76.32 mg. GAE/g.sample DW.
Additionally, susceptibility tests were performed by the disc diffusion method of Bauer et al. (1966)
with Mueller-Hinton agar (Difco). Zones of inhibition were measured after 24 hours of incubation at
370C. A bacteria culture was used to Mueller-Hinton agar plates evenly using a sterile swab. The test
organisms used in this study were as follows: Escherichia coli ATCC35218, Klebsiella pneumoniae
ATCC700603, Bacillus subtilis, Pseudomonas aeruginosa ATCC27853, Staphylococcus aureus
ATCC25923, Enterococcus faecalis ATCC 51299 and Listeria monocytogenes ATCC1911. Methanol
was used as a negative control while Ceftizoxime (ZOX 30 g), Penicillin (P 10U), Tetracycline (TE
30 g), Clindamycin (DA 2 g), Erythromycin (E 15 g), Chloramphenicol (C 30 g), Ofloxacin
(OFX 5 g) and Vancomycin (VA. 30 g) were used as positive controls. The antibacterial activities
were assessed by the presence or absence of inhibition zones and MIC values. The negative control
showed no inhibiting effect. The antimicrobial activity of GE extract was compared with standard
antibiotic disc and showed inhibition diameters ranging from 8 to 46 mm. The microbial strains
displayed a variable degree of susceptibility against the GE methanole extract. Antimicrobial activity
was observed against K. pneumoniae ATCC700603, B. subtilis, S. aureus ATCC25923 and E.
faecalis. The most susceptible bacteria to the G. macrorrhizum extract preparations was K.
pneumoniae ATCC700603 (13 mm).

Corresponding Author: MERAL ZTRK, meralztrk19@hotmail.com

1
UAK NVERSTES

906
KEYWORDS
Geranium macrorrhizum, Geraniaceae, Total phenolic content, Antimicrobial activity

907
 Poster Session 6
Submission ID: 812

ANTIBIOFILM EFFECTS OF THREE MACROFUNGI (MORCHELLA

ANGUSTICEPS, TRAMETES VERSICOLOR, LACTARIOUS
DELICIOUS) ON ENTEROCOCCUS FAECALIS

1 1 1 1 2
ARZU COLER CHAN , BASAR KARACA , AYSE BUSRA KARAKAYA , ILGAZ AKATA , ERGIN MURAT ALTUNER

ABSTRACT
It is commonly well known that 80% of all the bacterial infections are in relation with
biofilms. Due to common problems caused by biofilms, alternative antibofilm strategies must be
developed. Enterococcus strains are able to form complex surface-associated communities, called
biofilms, contributes to its resistance and persistence in both host and non-host environments.
Mushrooms are functional foods and a source of physiologically beneficial medicines. Possible
antibiofilm effects of medicinal mushrooms have also a notable potency against biofilms.
Additionally, increasing prevalence of infectious diseases is becoming a world wide problem, and the
resistance problem demands that novel antimicrobial agents originated from natural products such as
medicinal mushrooms to combat with the infections should be observed. The present study reports the
capacity of three medicinal methanolic macrofungi extracts (Lactarius deliciosus, Trametes versicolor,
Morchella angusticeps) to inhibit in vitro biofilm formation by Enterococcus faecalis ATCC 21599
strain. The strains antimicrobial susceptibility testing was performed by microdilution method. 6.25,
12.5, 25, 50, 100, and 200 mg/mL concentrations of three macrofungi extracts were selected to
perform MIC (Minimum Inhibition Concentration) test. For antibiofilm assays, the optimum biofilm
forming capacity of the strain was primarly confirmed under different incubation conditions such as
incubation period (24-48 h) and culture media (TSB medium was adjusted with the different
concentrations of glucose; 0, 0.25, 0.5, 1.0, and 1.25%) by microtiter biofilm assay. Quantification of
the antibiofilm effects of macrofungi was carried out by modifying the microtiter biofilm formation
protocol described by Stepanovic et al (2000). Briefly, in a U bottom 96 microplate, wells were filled
with 200 L different concentrations of methanolic macrofungi extracts (6.25, 12.5, 25, 50, 100, and
200 mg/mL) and at 5% (v/v) inoculum. Following this step, the microplate was incubated at 37 C for
48 h. To quantify biofilms, the each well was discarded and washed 3 times with sterile 0.85% NaCl
in order to remove non-adherent cells. The attached biofilm samples were fixed with 200 L of
methanol (95%) for 10 min. Methanol was then discarded and the wells were air dried. After that, 200
L of crystal violet solution (0.1%, prepared with distilled water) were added to the wells for 30 min.
Excess stain was removed by rinsing the plate under tap water and then air dried. The bounded dye
was dissolved with 70:30 ethanol-acetone solvent mixture for 15 min. The amount of dye was
quantified by measuring the Optical Density (OD) at 595 nm using a microtiter plate reader. Results
for this test were given as percentage of biofilm formation inhibition applying the following formula:
Biofilm formation inhibition percentage = [100-(ODassay/ODcontrol)] x 100. The MIC values were
determined as 50, 25, and 50 mg/mL for M. angusticeps, T. versicolor, and L. delicious respectively.
The optimum biofilm forming conditions for the strain were assigned as 48 h-incubation period and

Corresponding Author: ARZU COLER CHAN, arzucoleri@gmail.com

1
ANKARA UNVERSTY
2
KASTAMONU UNVERSTY

908
0.50% glucose concentration. The biofilms were sampled considering these conditions for antibiofilm
assays. While all macrofungi extracts had inhibitory effects on the biofilm formation of the strain, T.
versicolor extract was found to be the most effective with the highest reduction rates of 99.54, 100,
and 100% (50, 100, and 200 mg/mL, respectively). The results obtained showed that all the tested
mushroom extracts, but mostly the T. versicolor extract, had inhibitory effects on E. faecalis biofilm
production. As this report was the first on the inhibition of E. faecalis biofilms by these macrofungi
extracts, it can be concluded that medicinal macrofungi could be considered as candidates for novel
antibiofilm strategies.

KEYWORDS
Enterococcus faecalis, biofilm, antibiofilm, medicinal macrofungi

909
 Poster Session 6
Submission ID: 813

ANTIMICROBIAL EFFECT OF ESSENTIAL OIL EXTRACTS OF

ONION AND GARLIC

1 2 2
DIDEM SZERI , NIHAT AKIN , MEHMET DEMIRCI

ABSTRACT
Physical and chemical preservative methods were used for many years to protect foods from
spoilages and pathogens. Essential oil extracts which extracted from various part of plants have been
considered as natural preservatives or food additives. They are mixture of numerous components and
aromatic oily liquids. They also contain alkoloids, flavonoids, isoflavonoids, tannins, cumarins,
glycosides, terpens and organic acids which may have antimicrobial and antioxidant properties.
Distillation, extraction and pressing methods are used in extraction of essential oils from matrix.
Allium family have more than 500 species although have similar biochemical, nutraceutical and
phytochemical properties. Many are known to have antibacterial and antifungal effects because of
containing antioxidants, sulphur and phenolic components. Onion and garlic are members of Allium
family and also contain medically important organosulfur including components. The aim of this study
was to discussed area of utilization and antimicrobial effect of essential oil extract of onion and garlic.

KEYWORDS
onion, garlic, essential oil, antimicrobial

Corresponding Author: DDEM SZER, didem.sozeri@yahoo.com

1
NAMIK KEMAL NVERSTES
2
SELUK NVERSTES

910
 Poster Session 6
Submission ID: 814

USE OF AROMATIC PLANTS AND SPICES AS AN ALTERNATIVE

FEED ADDITIVE

1 1 1 1 2
AYSUN KOP , ALI YILDIRIM KORKUT , HATICE TEKOUL , HLYA SAYI , HARUN DIRAMAN

ABSTRACT
The rapid increase in the world population makes it necessary to use limited food sources
more efficiently. Today, it has been understood that a balanced diet is very important for people. The
production of aquatic products in the world and in Turkey is increasing every year and natural fish
stocks are decreasing due to various environmental factors. This suggests that the products obtained
through hunting can no longer be increased and that existing stocks must be protected. As a result, it is
inevitable that there will be an increase in the products obtained by aquaculture. Along with the
increasing amount of aquaculture, the feed sector has also gained great importance. In aquaculture,
feed constitutes 40-60% of the cost required for successful and healthy production. Fish feeds are
obtained by mixing animal (fish meal, fish oil, squid flour, crab flour, shrimp flour, fish silage etc.)
and vegetable (soybean flour and pulp, wheat, corn flour and gluten etc.) raw materials as well as feed
additives (Antioxidants, pigment substances, vitamin-mineral mixture, etc.) in varying proportions.
Fish meal is the most widely used protein source in fish feeds because of its good protein quality, its
rich energy and mineral properties, its high digestibility and its consumption by fish. Due to the fact
that the factories producing fish meal in Turkey are few and the nutritional content of fish flour is
inadequate, the importation of fish meal from abroad has begun to be started and the feed cost has
increased accordingly. Therefore, research on the use of alternative vegetable protein sources to reduce
the use of fish meal in fish feeds has begun to gain importance. As raw materials of alternative
vegetable origin which can be used in fish feed, algae, hazelnut meal, potato flour, duckweed, alfalfa
flour can be given. Alternative raw materials of animal origin are more limited, such as worm flour
and black fly flour. New alternative additives used in practice are enzymes, organic acids, probiotics,
oligosaccharides (prebiotics) and plant extracts. The aim of this study was to investigate the use of
natural and partially cheaper aromatic plants and spices such as fennel, marjoram, caraway,
chamomile and garlic as alternative feedstuffs and / or additives in previous national and international
publications and to evaluate the nutritional content of the morphological properties and its benefits and
to investigate its use in fish feeding.

KEYWORDS
Fish feed, Feed additives, aromatic plants, spice

Corresponding Author: HARUN DIRAMAN, hdiraman@aku.edu.tr

1
EGE NVERSTES, SU RNLER FAKLTES, YETTRCLK BLM, BORNOVA-ZMR
2
GIDA KONTROL ARATIRMA VE UYGULAMA MERKEZ, AFYON KOCATEPE NVERSTES

911
 Poster Session 6
Submission ID: 815

SYNERGISM BETWEEN LACTARIOUS DELICIOUS AND

VANCOMYCIN AGAINST VANCOMYCIN-RESISTANT
ENTEROCOCCUS FAECIUM

1 1 2 1 1
ARZU COLER CHAN , BASAR KARACA , ERGIN MURAT ALTUNER , ILGAZ AKATA , OZLEM YILDIRIM

ABSTRACT
Novel drug development strategies are needed to struggle antimicrobial resistance. The object
of our study is to highlight a strategy such as: treating Enterococcus faecium antibiotic resistance with
Lactarious delicious extract rather than individual antibiotics. In this respect, screening of
antimicrobial activity of medicinal and edible macrofungus L. deliciosus was performed against
vancomycin resistant Enterococcus faecium NJ-1 and this natural macrofungus extract and antibiotic
combinations were evaluated to treat E. faecium. Disk diffusion and micro dilution tests were
performed according to the National Committee for Clinical Laboratory Standards guidelines for
enterococci (NCCLS). Agar well diffusion and micro dilution tests were also performed with
methanolic (60%) extracts of L. delicious against E. faecium NJ-1 strain. Minimum bactericidal
concentrations (MBC) of vancomycin and L. delicious extract were also determined to maintain
combinative antibiotic/macrofungus antimicrobial studies. 99.9% logarithmic reduction of the
bacterial population was considered as MBC value for both antibiotic and L. delicious extract. While
perfoming the MBC assay, EC50 values were synchronously determined by performing plate count
assay and the logarithmic reductions of microbial populations under different concentrations of
antibiotic and macrofungus extracts were evaluated by XLSTAT 2017 software, in terms of
evaluating the effects of antibiotic/macrofungus extract combinations further. For developping an
approach in an effort to evaluate possible antimicrobial interactions (synergistic, antagonistic, additive
etc.), vancomycin/L. delicious extract was selected in different combinations. The possible
antimicrobial effects of combinative vancomycine/macrofungus extracts were quantified and
determined by the Compusyn software. The strain was found to be resistant to vancomycin with 6
mm (milimeter) zone diameter (disk diffusion) and with 128g/mL MIC value. 10 mg/mL and 50
mg/mL concentrations of methanolic medicinal L. delicious extracts were used to evaluate
antimicrobial susceptibility with agar well diffusion method. Both 10 mg/mL and 50 mg/mL extracts
of L. delicious were found to be effective against the strain with the inhibition zone diameters of 17
mm and 30 mm, respectively. 6.25 mg/mL concentration of L. delicious was found as MIC value
against the strain. 1024 g/mL of vancomycin concentration and 25 mg/mL of L. delicious extract
values were found to be as MBC values. EC50 values of vancomycin and L. delicous extract were
calculated as 145.4 g/mL and 12.41 mg/mL, respectively by XLTSAT software. 140, 145.4, and 150
g/mL concentraions of vancomycin, 10, 12.41, and 15 mg/mL concentrations of L. delicious, and all
possible dual combinations of these factors were applied to calculate dose effects and dose
response curves. Dose effects values were specified according to the log-reduction of microbial
population by using XLSTAT software. Obtained dose effect data were used to model the

Corresponding Author: ARZU COLER CHAN, arzucoleri@gmail.com

1
ANKARA UNVERSTY
2
KASTAMONU UNVERSTY

912
drug/macrofungus interaction by using Compusyn software. According to the dose response index
(DRI) and dose response curve data, there was an absolute synergism between the vancomycin and L.
delicious extract. As the aim in this study was to achieve synergistic therapeutic effect and dose
reduction, it was absolutely concluded that natural products, such as L. delicious, could be taken into
consideration to struggle with the antibiotic resistance in the context of combining medicinal
macrofungi extracts and commonly used antibiotics. This approach has a notable potency to develop
alternative clinical applications to struggle infectious diseases.

KEYWORDS
Medicinal macrofungus, Vancomycin-resistant enteroccocci, synergism, antimicrobial
resistance

913
 Poster Session 6
Submission ID: 816

TRUFFLES AND STUDIES OF FOREST GENERAL DIRECTORATE

1 2 3
ZGE DENL , ZGR BALCI , MURAT MASUN

ABSTRACT

D
Truffles have a special position in gourmet kitchens, due to their intensive aroma. Valuable
truffle species grow naturally in forests that are especially in Mediterranean ecosystem. The amount of
truffles, which are harvested from natural areas, is not enough to supply the demands. Therefore, many

TE
European countries like France, Spain, Italy, Norway and Sweden, United State of America, Australia
and many other countries like New Zealand carry out truffle cultivation. There are many families in
Europe engaged in cultivation of truffles and transferring it from generation to the next. Cultivation of
truffles is more complex than typical mushroom cultivation. The cultivation of this mushroom which
EN
is the fruit of the mycorrhizal collaboration between forest trees and fungus needs specific seedling
propagation techniques. The most valuable truffle species are Tuber magnatum, T. melanosporum and
Tuber aestivum which can all be cultivated except T. magnatum. T. aestivum can be collected from
natural Red pine-oak and Black pine-oak mixed forests in Turkey. Truffle hunting has its own unique
methods, because the fungus matures under the soil and never appears on the soil surface. Till 2014,
ES
local people who live in naturally truffle distributed regions did not have the tradition of collection,
consume and trade in Turkey. The General Directorate of Forestry prepared the Truffle Action Plan in
2014 with the recognition of that truffles naturally grow in many parts of Turkey but were not know
by the people. The aim of the action plan is to stimulate the collection truffles from nature, and
PR

increase the knowledge and awareness of local people and entrepreneur to establish the specific
plantations for truffle cultivation. In this work, the studies which were carried out by the General
Directorate of Forestry related to truffles and the plans which are under consideration for future
activities are explained. Additionally, the fields where truffle mushrooms are grown in Turkey, the
actual situation of truffle market in the world, and ecological needs of truffle mushrooms are given.
T

KEYWORDS
O

truffle, mushroom, mycorrhizae, cultivation, oak, tuber

N

Corresponding Author: ZGE DENL, ozgedenlii@gmail.com

1
BATI AKDENZ ORMANCILIK ARATIRMA ENSTTS MDRL
2
ORMAN GENEL MDRL
3
DENZL ORMAN BLGE MDRL

914
 Poster Session 6
Submission ID: 817

USE OF AROMATIC PLANT EXTRACTS IN CHEESE PRODUCTION

1 1
NEE BADAK , YUSUF TUNTRK

ABSTRACT

D
Nowadays, the use of natural additives as food preservatives has become popular due to
concern for synthetic chemical additives and consumer conscious. There is great interest in developing
new techniques to make foods safer and more natural. High quality flavored cheeses containing some

TE
aromatic vegetable such as sage, thyme or rosemary have been described as suitable in studies.
Aromatic plants and their essential oils can be used as antimicrobial and antioxidants agents and also
some of them can prevent cheese blowing. Cheeses are traditionally affected by common paste defects
known as early and late blowing. Early blowing is caused by coliform bacteria such as Escherichia
EN
coli, Enterobacter aerogenes and late blowing is caused by butyric acid fermentation of the clostridial
spores present in raw milk. The use of certain aromatic plants as antimicrobial agent can prevent
blowing defect and innovative cheese covers may help to prevent the external growth of fungal
spoilers and thus avoid consumer exposure to mycotoxins. In addition, recent research on spices and
aromatic herbs suggests that they may be more effective in improving flavour and preserving food
ES
than artificial flavourings.

KEYWORDS
PR

aromatic plant, cheese blowing, antimicrobial aromatic plants, natural food preserving
T
O
N

Corresponding Author: NEE BADAK, nnesekaraca@gmail.com

1
YZNC YIL NVERSTES

915
 Poster Session 6
Submission ID: 818

EVALUATION OF PLEUROTUS OSTREATUS EXTRACTS FOR ITS

POTENTIAL ANTIBIOFILM ACTIVITY

1 1 1 1
BASAR KARACA , NAZNOOSH SHOMALI MOGHADDAM , AYSE BUSRA KARAKAYA , ARZU COLER CHAN ,
1
OZLEM YILDIRIM

ABSTRACT
Biofilms are structures formed by aggregates of bacterial cells on both biotic and abiotic
surfaces. These naturally existing biofilms can cause major risks to human beings, as 80% of the
bacterial infections were obtained by biofilms. Biofilms are major causes of nosocomial infections and
the bacterial attachment to some surfaces and the subsequent biofilm formation are important steps in
the establishment of chronic infections. In the food industry, biofilms can be a source of recalcitrant
contaminations, causing food spoilage, and are possible sources of public health problems. The reason
why bacteria growing in biofilm structures attain antimicrobial resistance is still unknown. Therefore,
the resistance of biofilm cells to antimicrobial agents make biofilms important on behalf of
discovering and developing novel and natural antibiofilm and antimicrobial agents. In this context, a
common edible mushroom, Pleurotus ostreatus was evaluated with respect to its antibiofilm activities
on Gram-positive and Gram-negative pathogens. Pseudomonas aeruginosa ATCC 27853, Escherichia
coli ATCC 25922, Staphylococcus epidermidis ATCC 35984, and methicillin-resistanst
Staphylococcus aureus (MRSA) ATCC 43300 strains were used both antimicrobial and antibiofilm
assays and were routinely cultured in Tyriptic Soy Broth (TSB) at 35C . A total of 20 g P. ostreatus
dried samples were powdered and treated with 200 mL of 95% ethanol under shaking conditions at
room temperature for 24 h. Then, the ethanolic extract was filtered through Whatman No. 4 paper, and
finally, the filtrate was lyophilized. The lyophilized extract was resuspended in sterile distilled water
with a final concentration of 200 mg/mL. The other extraction protocol was carried out with hot water.
20 mg of the dried sample was boiled in 200 mL of distilled water for 2 h. These two suspensions
were used in both antimicrobial and antibiofilm assays. Biofilm production conditions were optimized
for all strains before antibiofilm assays. Cultivation under low osmolar conditions (Luria-Bertanii
broth without NaCl) at 28C for E. coli, cultivation in TSB medium supported with 3% NaCl, and
1.0% glucose at 35C for S. aureus, and S. epidermidis, cultivation in TSB medium supported with
0.5% glucose for P. aeruginosa were preferred to increase their biofilm productions. Antimicrobial
assay was performed according to the microdilution method (NCCLS). Antibiofilm assays were
performed according to the Stepanovic et al. (2000) with slight modifications. Briefly, the TSB broths,
prepared according to the optimal biofilm forming conditions of the pathogens and different
concentrations of ethanolic and hot water extracts of P. ostreatus (0, 6.25, 12.5, 25, 50, and 100
mg/mL) were transferred to the U bottom 96 well microtiter plates with a final concentration of 200
L. The plates containing E. coli cultures were incubated at 28C, whereas other bacterial cultures
were incubated at 35C for 24 h. The wells, containing only bacterial inocula, and the wells containing
only test media were considered as positive and negative controls, respectively. At the end of the
incubation period, crystal violet binding assay was performed to determine the potential antibiofilm

Corresponding Author: BASAR KARACA, karaca@ankara.edu.tr

1
ANKARA UNVERSTY

916
activities of these extracts. Briefly, the plates were aspirated and washed with sterile physiological
serum for three times. The dried plates were filled with 200 L of 95% methanol and emptied again.
Following this step, the plates were filled with 0.1% crystal violet solution and incubated for 30 min at
room temperatures. Finally, the plates were rinsed under running tap water and each well was filled
with 200 L of an ethanol:acetone solvent mixture (70:30) to dissolve the bounded dye. The dissolved
dye was quantified by measuring the Optical Density (OD) at 595 nm using a microplate reader.
Results for this test were given as the percentage of biofilm formation inhibition using the following
formula: Biofilm formation inhibition percentage = [100-(ODassay/ODcontrol)] x 100. There was not
a significant antimicrobial activity on all the tested bacteria, but a significant antibiofilm acitivity of P.
ostreatus was observed. The ethanolic extract of the mushroom sample was found to be more
inhibitive on biofilm formation than hot water extract. The highest antibiofilm effect was detected on
MRSA for both ethanolic and hot water extracts (almost 100% reduction). The lowest antibiofilm
effect was detected on P. aeruginosa ATCC 27853 strain for the ethanolic extract of the mushroom
sample having a reduction rate of 45.78%. P. ostreatus is an edible mushroom that also has high
medicinal value such as its antimicrobial effect. In this study, P. ostreatus was tested for its ability to
inhibit the in vitro biofilm formation of common pathogens for the first time. It can be certainly
concluded that this mushroom should be taken into consideration to develop novel antibiofilm
strategies.

KEYWORDS
Pleurotus ostreatus, antibiofilm, antimicrobial, biofilm formation

917
 Poster Session 6
Submission ID: 819

USE OF BLACK SEED IN FOODS AS A NATURAL ADDITIVE

1 1
YUSUF TUNTRK , NEE BADAK

ABSTRACT

D
Nowadays, there is a tendency for the use of additives which will not affect the structure,
appearance, taste and smell of foods negatively and will protect the microbial, physical and chemical
properties of foods. Synthetic antioxidants have been used to prevent oxidation, which is one of the

TE
most important problems that can occur during the storage of foods, and to extend the shelf life of
foods. In recent years, due to their toxicological and carcinogenic properties synthetic antioxidants
added to food has been avoided. The most important feature of antioxidants to be added to food is
being natural, not synthetic. Another criterion for the use of antioxidants is the low cost of
EN
antioxidants, which is an important factor in consumer preference. One of the plants with antioxidant
effect is the essential oil of black seed. Black seed is a valuable medicine and spice plant of
Ranunculaceae family whose botanical name is Nigella sativa L. and used for more than 2000 years.
Seed, oil and seed components - mainly thymoquinone - are traditionally used because of their potent
therapeutic properties. It has attracted attention recently due to its antimicrobial, antioxidant,
ES
antiinflammatory, gastroprotective, antidiabetic, antitumor, hepatoprotective, immune system
strengthening effects and contains important fatty acids, vitamins, minerals and volatile components.
Black seed is widely consumed worldwide as food and spices due to its nutritious, flavorer and
ornamental qualities.
PR

KEYWORDS
black seed, natural additives, food antioxidant, aromatic oils
T
O
N

Corresponding Author: NEE BADAK, nnesekaraca@gmail.com

1
YZNC YIL NVERSTES

918
 Poster Session 6
Submission ID: 820

TOTAL PHENOLIC CONTENT AND ANTIMICROBIAL ACTIVITY OF

METHANOL EXTRACT OF STACHYS OBLIQUA WALDST. & KIT.
AND S. THRACIA DAVIDOV (LAMIACEAE) IN TURKEY

1 1 1 1 1
S. ELIF KORCAN , YASEMIN S. KARAFAKIOLU , BRAHIM BULDUK , HAVA KLEMEK , MERAL ZTRK ,
1
AHMET KAHRAMAN

ABSTRACT
Stachys L. is one of the largest genera of the family Lamiaceae with about 300 taxa. Stachys is
a taxonomically complex and widely distributed genus in the temperate regions of the world. Many
specie of Stachys mainly grow in rocky areas. In Turkey, it is represented by 110 taxa, 51 of which are
endemic. S. obliqua Waldst. & Kit. is a perennial plant with erect stems, oblong-lanceolate basal
leaves, mostly remote verticillasters, subcampanulate sepals and pale yellow petals. S. thracica
Davidov is a perennial plant with erect or ascending stems, oblong or oblonglanceolate to lanceolate
basal leaves, distant verticillasters, subcampanulate sepals and purple petals. Phytochemical
investigations on Stachys species have shown the occurrence of flavonoids, diterpenes, phenyl
ethanoid glycosides and saponins. They have been reported to treat genital tumors, sclerosis of the
spleen, inflammatory tumors and cancerous ulcers. The aim of this work is to comprehensively
investigate total phenolic content and antimicrobial activity of methanol extracts of S. obliqua and S.
thracica naturally growing in Turkey. The level of total phenols in methanol extracts was determined
by using FolinCiocalteu reagent and external calibration with gallic acid. The absorbance was
measured at 760 nm using a spectrophotometer. The concentration of the total phenolics was
calculated as mg of gallic acid equivalent by using an equation obtained from gallic acid calibration
curve. The total amount of phenolic substance in the extracts was 35.76 mg. GAE/g.sample DW.
Susceptibility tests were also performed by the disc diffusion method of Bauer et al. (1966) with
Mueller-Hinton agar (Difco). Zones of inhibition were measured after 24 hours of incubation at 370C.
A bacteria culture was used to Mueller-Hinton agar plates evenly using a sterile swab. The test
organisms used in this study were as follows: Escherichia coli ATCC35218, Klebsiella pneumoniae
ATCC700603, Bacillus subtilis, Pseudomonas aeruginosa ATCC27853, Staphylococcus aureus
ATCC25923, Enterococcus faecalis ATCC 51299 and Listeria monocytogenes ATCC1911. Methanol
was used as a negative control while Ceftizoxime (ZOX 30 g), Penicillin (P 10U), Tetracycline (TE
30 g), Clindamycin (DA 2 g), Erythromycin (E 15 g), Chloramphenicol (C 30 g), Ofloxacin
(OFX 5 g) and Vancomycin (VA. 30 g) were used as positive controls. The antibacterial activities
were assessed by the presence or absence of inhibition zones and MIC values. The negative control
showed no inhibiting effect. The antimicrobial activity of extract of the species studied was compared
with standard antibiotic disc and showed inhibition diameters ranging from 7 to 18 mm. The microbial
strains displayed a variable degree of susceptibility against the methanole extract of the species
examined. Antimicrobial activity was observed against K. pneumoniae ATCC700603, S. aureus

Corresponding Author: HAVA KLEMEK, hava.klmk@gmail.com

1
UAK NVERSTES

919
ATCC25923 and E. faecalis. The most susceptible bacteria to their extract preparations was S. aureus
ATCC25923 (17 mm).

KEYWORDS
Stachys, Lamiaceae, Total phenolic content, Antimicrobial activity

920
 Poster Session 6
Submission ID: 821

THE INVESTIGATION OF GENOTOXIC EFFECTS OF SITAGLIPTIN

THE ACTIVE INGREDIENT OF AN ANTIDIABETIC DRUG USING
CHROMOSOMAL ABERRATION TEST

1 1 1
DENIZ YZBAIOLU , CEMILE ENGZEL , FATMA NAL

ABSTRACT
Sitagliptin, the active ingredient of an anti-diabetic drug, is used in the treatment of Typ 2
diabetes mellitus. The aim of the present study was to investigate the ability of Sitagliptin to induce
chromosomal aberrations (CAs) in human lymphocytes in vitro. The effect of Sitagliptin on mitotic
index (MI) was also evaluated. 31.25, 62.50, 125.00, 250.00, 500.00, 1000.00 g/mL concentrations of
Sitagliptin were used. A negative, a solvent (25% DMSO) and a positive control (MMC) were also
included. This study was approved by the ethical committee of Gazi University, Faculty of Medicine
(14.11.2012-363). Sitagliptin significantly increased the frequency of CAs at 125.00, 500.00, and
1000.00 g/mL concentrations for 24 h and at all the concentrations for 48 h treatment compared to
the negative control. MI significantly decreased at the highest concentration (1000.00 g/mL) of
Sitagliptin for 24 h treatment and at all the concentrations of Sitagliptin (except for 31.25 g/mL) for
48 h treatment compared to the negative control. Our results demonstrate that Sitagliptin have
clastogenic and cytotoxic effects on human lymphocytes in vitro. Acknowledgment: This study was
supported by TUBITAK under the project number 212T256.

KEYWORDS
Sitagliptin, Genotoxicity, Chromosomal aberrations, Mitotic index

Corresponding Author: DENZ YZBAIOLU, deniz@gazi.edu.tr

1
GAZ NVERSTES, FEN FAKLTES, BYOLOJ BLM, GENETK TOKSKOLOJ LABORATUARI, 06500,
ANKARA, TRKYE

921
 Poster Session 6
Submission ID: 822

INVESTIGATION OF RETINOL (VITAMIN A) POTENTIAL

ANTIGENOTOXIC EFFECTS ON MITOMYCIN-C GENOTOXICITY
BY CHROMOSOMAL ABERRATION TEST

1 1 1 1
KBRA SEVIMLI-CAN , DENIZ YZBAIOLU , ECE AVULOLU-YILMAZ , FATMA NAL

ABSTRACT
Vitamin-A (Vit-A) has a supporting effect on skin and immune system and protects against
harmful effect of pro-oxidants. The free radical scavenging effect of Vit-A is ineffective to neutralize
of singlet oxygen (1O2), but it is considered to be a strong antioxidant since it can affect the level of
other antioxidants in the tissue. In this study, potential antigenotoxic effect of Vit-A (retinol) was
investigated in human peripheral lymphocytes (HPLs) by chromosomal aberration (CA) test against
Mitomycin-C (MMC), an antitumor agent. Different concentrations of retinol (0.05, 0.010, 0.015, and
0.020 g/mL) were used in combination with Mitomycin-C. Three treatment procedures were applied
for 24 h and 48 h; pre-, simultaneous and post-treatment. At the 24 h pre-treatment with retinol, the
percentage of abnormal cells and the frequency of abnormality significantly decreased in all the
concentrations compared to positive control. This decrease was significant at the two highest
concentrations at simultaneous treatment and at the only highest concentration at post- treatment. At
48 h, in all the treatments and concentrations (except 0.05+MMC g/mL in simultaneous treatment)
the frequency of abnormality and percentage of abnormal cells reduced compared to positive control.
These results indicates that retinol reduced the frequency of CAs induced by MMC at all the treatment
types. It can be concluded from these results that retinol has protective and ameliorating effect against
MMC induced genotoxicity in vitro human lymphocytes.

KEYWORDS
Retinol, Antigenotoxicity, Chromosomal aberration

Corresponding Author: DENZ YZBAIOLU, deniz@gazi.edu.tr

1
GAZ NVERSTES, FEN FAKLTES, BYOLOJ BLM, GENETK TOKSKOLOJ LABORATUARI, 06500,
ANKARA, TRKYE

922
 Poster Session 6
Submission ID: 824

QUALITY EVALUATION OF FLAXSEED AND BLACK CUMIN OIL

OBTAINED BY MICROWAVE AND ULTRASONIC EXTRACTION

1 1
FETHIYE TAKADA , ONUR DKER

ABSTRACT
The growing interest in green chemistry requires fresh perspectives on analytical extractions
on the medicinal and aromatic plants. Reduced solvent consumption, alternative safer solvents, and
reasonable energy demands must be balanced with traditional analytical considerations such as
extraction yield and selectively [1]. Especially medicinal and aromatic plant extraction methods tend
to use of green solvent and new extraction technologies instead of the traditional methods. Studies in
recent years, different extraction methods of plant extraction have been become crucial. Some of those
supercritical fluid extraction, microwave extraction, ultrasonic extraction and accelerated solvent
extraction [2,3]. In this study, the effect of different extraction technologies on flaxseed oil and black
cumin oil composition will be aimed. However, these investigations are needed for the appropriate
assessment of the prospective of these novel techniques. Also, black cumin seed oil and flaxseed oil is
quite valuable in terms of the linoleic acid and oleic acid. The presence of these bioactive compounds
helps in the prevention of cardiovascular diseases, diabetes, memory loss, and constipation. In
accordance with the aim of this work, the oils will be extracted by microwave extraction and ultrasonic
extraction with ethanol and oil composition will be analyzed. Consequently, for these seeds, which are
wealthy in terms of fatty acid, an alternative extraction method will be investigation. [1] Essel, Victor,
and Douglas E. Raynie. "Green Chemistry Perspectives on Analytical Extractions." Lc Gc North
America, 31 (2013): 18-21. [2] Khattab, Rabie Y., and Mohammad A. Zeitoun. "Quality evaluation of
flaxseed oil obtained by different extraction techniques." LWT-Food Science and Technology 53.1
(2013): 338-345. [3] Bakhshabadi, Hamid, et al. "The effect of microwave pretreatment on some
physico-chemical properties and bioactivity of Black cumin seeds oil." Industrial Crops and Products
97 (2017): 1-9.

KEYWORDS
fatty acid, flax seed, black cumin, microwave, ultrasonic extraction

Corresponding Author: FETHYE TAKADA, takadasfethiye@gmail.com

1
MERSN UNVERSTY

923
 Poster Session 6
Submission ID: 826

EFFECT OF LAVANDULA ANGUSTIFOLIA ESSENTIAL OILS ON

SOME RHEOLOGICAL PROPERTIES (ALVEOGRAPH AND
FARINOGRAPH) OF DOUGH

1 2 3 4
HARUN DIRAMAN , YUNUS EMRE GKE , TURGAY ANAL , AMIR SOLTANBEIGI

D
ABSTRACT

TE
The effects of Lavandula angustifolia essential oils on rheological profiles (alveograph and
farinograph characteristics) and also, some chemical properties (moisture content 12.1 %, ash content
0.73 %, total protein content 12.90 %, wet gluten 31 %, Perten Gluten Index 90 %, standard and
modified sedimentation tests 39 ml and 62 ml respectively, before essential oil addition) for flour

EN
quality were investigated. There is no detailed study concerning the effect of essential oils of L.
angustifolia on rheological properties (alveograph and farinograph) of dough. Aromatic profile of
essential oil obtained by a hydrodistilation method from Lavandula angustifolia (leave + flower)
grown in Afyonkarahisar Turkey (Medicinal and Aromatic Plant Center), was determined by GC-
MS (Agilent 7890 B) and this volatil oil contained as major components 1,8 Cineole (59.446 %),
ES
camphor (7.202 %), Linaol (5.368 %), -pinene (2.950), dl-linomenen (2.337)%) and -pinene (1.460
%), respectively. This essential oil from Lavandula angustifolia was added to dough as % 0.2 in the
course of mixing. The purpose of the present study was to elucidate the effect of essential oils (0.2 %
Lavandula angustifolia) on the alveograph and farinograph characteristics of wheat dough. The
standard and modified Zeleny sedimentation values and all farinograph data (except for development
PR

time) were generally not affected by adding with essential oil (Lavandula angustifolia). These results
on dough rheological were rewardigly found based on alveograph (increasing W [213 -230 Joules] and
L [34 -38 mm]) and farinograph data(increasing development time 2.29 7.49 and Falling Quality
Number 116-122 ).
T

KEYWORDS
O

Lavandula angustifolia, essential oil, alveograph, farinograph

N

Corresponding Author: HARUN DIRAMAN, hdiraman@aku.edu.tr

1
AFYON KOCATEPE NVERSTES MHENDSLK FAK. GIDA MHENDSL & GIDA KONTROL
ARATIRMA VE UYGULAMA MERKEZ, AFYONKARAHSAR
2
AFYON KOCATEPE NVERSTES. FEN BLMLER ENSTTS. GIDA MHENDSL ABD.
AFYONKARAHSAR
3
TARLA BTKLER ERKEZ ARATIRMA ENSTTS. GIDA VE KALTE DEERLENDRME BLM.
YENMAHALLE ANKARA
4
AFYON KOCATEPE NVERSTES GIDA KONTROL ARATIRMA VE UYGULAMA MERKEZ,
AFYONKARAHSAR

924
 Poster Session 6
Submission ID: 827

INFLUENCE OF DIFFERENT CARBON SOURCES ON GROWTH AND

ROSMARINIC ACID ACCUMULATION OF THYMUS
LEUCOTRICHUS HAL. SHOOT CULTURES

1 2 1 3
TUBA BEKRCAN , MUSTAFA CCE , SERCAN YILDIRIM , ATALAY SKMEN

ABSTRACT
Aim of the study: The aim of this study was to examine the effect of different concentrations
of sucrose on growth and rosmarinic acid accumulations in vitro grown Thymus leucotrichus Hal.
Material and Methods: Four different groups were obtained by changing the sucrose concentrations of
the MS medium which include 2 mg/L 2ip and 0.1 mg/L NAA. Cultures in sucrose-containing (10%,
20%, 30%, 40%) media were evaluated 4 weeks later. Number of shoot and node, shoot length, fresh
and dry weight of Thymus leucotrichus seedling were measured. In addition, the in vitro grown
seedlings were dried and subjected to HPLC analysis to determine the amount of rosmarinic acid.
Results: The highest shoot length(34.00 2.83 mm ) and number of nodes (6.47 0.68) were
observed at 10% sucrose concentration. The highest number of shoots was observed in the medium
containing 30% sucrose with 4.47 0.51 . When the fresh (114.8 11.2 mg) and dry weight (18.05
1.6 mg) parameters were examined, the most effective group was 40% sucrose concentration. While
rosmarinic acid content of natural seedling was 6.78mg/g dry weight the highest rosmarinic acid value
observed in the experimental groups was 19.10 1.10 mg / g dyr weigth at 40% sucrose
concentration. As the sucrose concentration increased, the amount of rosmarinic acid was found to be
statistically significantly increased

KEYWORDS
T. leucotrichus, in vitro, sucrose, HPLC, rosmarinic acid

Corresponding Author: TUBA BEKRCAN, tubabekircan@gmail.com

1
KARADENZ TEKNK NVERSTES
2
GRESUN NVERSTES
3
KONYA GIDA VE TARIM NVERSTES

925
 Poster Session 6
Submission ID: 828

PHYTOESTROGENS EFFECTS ON CARDIOVASCULAR DISEASES

1 1 1 1 1 1
GLIZER ELK , MBERA L , BRA GZ , EZGI TOPTA BIYIKLI , ALI EMRAH BIYIKLI , ELMAS ERSZ

ABSTRACT
Cardiovascular diseases have the most incidence density all over the world which are
originated from oxidative stress, reactive oxygen production and peroxidation of lipids. In order to
prevent this disease, decrease its complications or inhibit its development diet has an important factor.
Phytoestrogens which are a kind of functional nutrition, act as a guardian against oxidative stress on
the vascular cells. This property of phytoestrogens is coming from the endothelial nitric acid synthesis,
thereby stimulating the vasodilatation activity. In addition to this, phytoestrogens provide anti
thrombotic and anti atherogenic effects. OBJECTIVE: In this review study, phytoestrogens
cardiovascular risk factors, serum lipid levels, inflammatory reagents and arterial hardness effects are
investigated. METHOD: According to the up to date literature research, the influence of
phytoestrogens which are naturally occurring on the food or supplementary phytoestrogens on
cardiovascular diseases are investigated. RESULTS: Soya, whichs phytoestrogen contribution is too
high, is consumed too much in Asia territory. According to this, the cardiovascular disease rate in Asia
is too low with respect to western populations. Phytoestrogens, especially increase the isoflavone
groups anti oxidative activity and nitric oxides bioavailability and thereby provides vasodilatation and
prevents vascular cell damage. In addition to this, it decreases liver and/or total serum triglyceride
level and/or LDL cholesterol, and it increases HDL cholesterol and/or HDL/LDL cholesterol rate.
Therefore it has positive effects on cardiovascular disease risk factors. Phytoestrogens contribute to
the vascular consistency and helps to prevent hypertension which is one of the important risk factor
due to atherosclerosis. In some studies it is mentioned that, there is a reverse relationship between
dietary isoflavones and aorta hardness. However, there are also some studies which provide that, the
isoflavones coming from soya and red trefoil has no influence on cholesterol levels. CONCLUSION:
Phytoestrogens could be one of the important nutrition for preventing cardiovascular diseases which
are the most common public health issue. However, there should be more studies made on this issue,
in order to understand its effects more clear and the amount should be consumed for these type of
illnesses.

KEYWORDS
Phytoestrogens, soya, cardiovascular diseases.

Corresponding Author: GLZER ELK, diyetisyen.72@gmail.com

1
SELUK NVERSTES AKEHR KADR YALLAGZ SALIK YKSEK OKULU

926
 Poster Session 6
Submission ID: 829

DETERMINATION OF FACTORS AFFECTING INDIVIDUALS'

THYME CONSUMPTION (THE CASE OF MANISA PROVINCE OF
SALIHLI DISTRICT)

1 1
RVEYDA KIZILOLU , SARA NILDA ATAOLU

ABSTRACT
Total spice plant production in Turkey is 38341 tons for the year 2016. Of these 48.48% are
caraway, 38.40% are thyme, 6.59% are black seed, 6.43% are fennel and 0.11% are coriander. A total
of 14724 tons of thyme were produced in Turkey in 2016. Thyme was produced 1585 tons in TR33
(Manisa-Afyon-Ktahya-Usak) region and 828 tons of in Manisa province. In other words, the
production of Manisa is generated thyme constitutes 5.62% of Turkey and 52.24% of the region. With
752 tons of production, Salihli is firstly found in 90.82% of the thyme production. Salihli produces
47.44% of the region's thyme production and 5.11% of Turkey. In this context, it is aimed to
determine of the individuals in Salihli Province, which is one of the most produced places of the
region and province the level of thyme, which is one of the medicinal aromatic plants, consumption
consciousness and factors affecting thyme consumption. This was discussed with 272 individuals
determined by proportional sampling. The average age of the interviewed individuals was found to be
36.07 age, 52.21% female, 63.60% single and average income was 2,634.77 TL / month. The first ten
medicinal aromatic plants that individuals bought; sage (%85.29), mint (%74.26), thyme (%73.53),
tilia (%70.96), garlic (%68.75), nettle (%61.40), dill (%58.46), hibiscus (%49.63), cumin (%47.43)
and nigella (%45.96). Thyme, which is the third choice for individuals to consume consumption is
59.10 grams per month. Binary logit analysis was used to determine the factors that affect the thyme
consumption of individuals. A dummy variable is used as a dependent variable for consuming or not
consuming thyme. As an explanatory variable; the age of the individual, gender, marital status,
education status, monthly income, the number of individuals in the family, the state of knowing the
definition of medical aromatic plants, use purpose (treatment and odor-taste), consumed amount,
purchasing frequency (weekly and monthly), preferred shape (open and packed) and the factors to be
considered when purchasing (harvest time, color, smell, freshness, drying environment, amount of
drying and amount of moisture) were modeled. As a result of the analysis, it was determined that
thyme consumption at the 5% significance level negatively affected the gender and the product
positively affected the open purchase and drying environment. That means that women may consume
9% more thyme than men. One unit increase in thyme consumption is expected to increase by 14%
when buying thyme, rather than buying it openly. In the same way, consumers consume about 11% of
the drying environment compared to those who do not consume. It was observed that those who prefer
to use for treatment, use for taste, and purchase once a week, which are meaningful at the 1%
significance level, are positively related to consumption. One unit increase in those who prefer to
consume is a 45% increase in therapeutic use. That is, consumers consume 45% probability of
treatment according to their consumption. It has been determined that 21% of the thyme is consumed

Corresponding Author: SARA NLDA ATAOLU, arasadlin25@gmail.com

1
GAZOSMANPAA NVERSTES

927
for taste and odor. One unit increase in the likelihood of consuming another tag is expected to increase
the likelihood of choice for taste and odor by 21%. The likelihood of consuming in individuals is
directly proportional to the increase in the purchase frequency of thyme. That is, a unit increase in the
likelihood of consumption is expected to increase the frequency of purchases per week by 21%. It
gives the result that the individual prefers to consume freshly or take it as much as they need to buy
thyme.

KEYWORDS
Consciousness Level, Binary Logit Analysis, Thyme, Preference, Consumption

928
 Poster Session 6
Submission ID: 830

PHYTOESTROGENS AND THYROID FUNCTIONS

1 1 1 1 1 1
GLIZER ELK , BRA GZ , MBERA L , EZGI TOPTA BIYIKLI , ELMAS ERSZ , ALI EMRAH BIYIKLI

ABSTRACT
Thyroid gland is an organ which regulates the metabolic functions via hormones. Heredity,
microorganisms, aging, iodine deficiency or over iodine and some of the medicines could cause
thyroid originated illnesses. In addition to these, some endocrine damagers may negatively influence
thyroid functions. Phytoestrogens are natural endocrine damagers. A phytoestrogen called genistein
effects thyroid functions negatively. Therefore studies made to understand this issue. OBJECTIVE: In
this study, thyroid autoantibody, subclinical hypothyroid parameters are investigated with their
interactions between phytoestrogens. METHOD: Phytoestrogen supplementation and its effects to
thyroid related sicknesses are investigated through literature research. RESULTS: Most of the studies
point that, phytoestrogens effect the connections of thyroid hormone receptors to the thyroid hormone
and negatively effects the thyroid hormone signals. Guanthyrogenic effects of genistein is coming
from isoflavones thyroid hormone synthesis, its metabolism and thyroid hormone transport proteins.
Some invivo and invitro studies shows that, genistein inhibits the thyroid peroxidase (TPO) which
have an important role in synthesis of thyroid hormone, and decreases the thyroid hormone levels in
the circulating system. Soya, which contains phytoestrogen, consumption negatively influences the
thyroid functions. Although there are some studies which reveal that, phytoestrogen consumption on
healthy or thyroid sickness persons has no reasonable effects. CONCLUSION: Phytoestrogens have
lots of positive effects on healthy people. However it shows negative influences on thyroid
metabolism. But at some studies it has no effect on thyroid functions. In order to enlighten this topic,
there should be more studies made.

KEYWORDS
Phytoestrogen, genistein, nutrition, thyroid

Corresponding Author: GLZER ELK, diyetisyen.72@gmail.com

1
SELUK NVERSTES AKEHR KADR YALLAGZ SALIK YKSEK OKULU

929
 Poster Session 6
Submission ID: 831

DETERMINATION OF TOTAL PHENOLIC, TOTAL FLOVANOID,

CONDENSED TANNINS AND ANTIOXIDANT ACTIVITY OF
KARAALI FRUITS (PALIURUS SPINA CHRISTI)

1 2 2
KASIM TAKIM , MESUT IIK , ADEM NECIP

ABSTRACT
Paliurus spina-christi (PSC) plant is used against the antidiarrhea, diuretic and rheumatism
among the population. In addition, the plant's samara-type fruits are used as anti-inflammatory agents
against kidney stones, chest and eye infections, and the leaves are externally used for inflammation of
the uterus [1]. There are five known species of plant, a member of the Rhamnaceae family. In Turkey,
there are only P. spina-christi Mill from these five species [1]. P The PSC (blackcurrant) plant is a
very well known plant in Asia and the Mediterranean region. Southern Europe, Crimea, Caucasus,
Western Syria, North-South-West Iran and Northern Iraq. Where the forests are destroyed, the
invaders are in frequent bushes and woodlands. The zigzag branch, which grows in almost all Anatolia
in Turkey, is seen as a thorny hedge with a height of two to three meters. Leaves are ovate, stipules are
thorn-like, flowers are yellow, fruits are circle; Flat, winged, three-seeded and dry [3]. In this study on
the mature fruit of the PSC known to be biologically active, Total phenolics, total floovoid and
condensed tannin analysis of the fruits used as part of the plant were investigated and the antioxidant
activity was investigated accordingly. The total phenolic compound content of the PSC extracts
prepared by the deoxygenation method was found to be 22,10 0,09 mg Gallic Acid Equivalent / g
dry PSC. Total amount of flavonoid compound: 8,29 0,07 mg Quercetin Equivalent / g dry PSC. The
amount of compound in the condensate of PSC extracts was determined to correspond to 238,11 2
mg Tannic Acid Equivalent / kg dry PSC. The total antioxidant capacity of PSC was determined by
Cuprac method and found to be 10.47 0.07 mg Trolax Equivalent / g dry PSC. This result shows that
Trolox, Ascorbic acid and BHT, which are standard antioxidant compounds and used for comparison,
have higher activity. In addition, the DPPH method was used to determine the specific antioxidant
properties of PSC, and consequently PSC was reduced to 63.69 1.32% at 30 g / mL with gallic acid
as standard antioxidant compounds (66.33% 2.11), BHT (42.44 1.87%) showed higher radical
reduction activity than trolax (23.10 0.91) and ascorbic acid (28.38 1.12%).

KEYWORDS
Paliurus spina-christi, antioxidant, tannin, phenolic

Corresponding Author: KASIM TAKIM, kasimtakim@harran.edu.tr

1
HARRAN NVERSTES VETERNER FAKLTES
2
HARRAN NVERSTES SALIK MESLEK YKSEK OKULU

930
 Poster Session 6
Submission ID: 833

ANTIHYPERTENSIVES AND GENOTOXICITY

1 1 1
DENIZ YZBAIOLU , ECE AVULOLU-YILMAZ , FATMA NAL

ABSTRACT
Hypertension known as high blood pressure is the most common cardiovascular disease in the
world. Hypertension usually does not cause symptoms initially, but sustained hypertension over time
is a major risk factor for hypertensive heart disease, coronary artery disease, stroke, aortic aneurysm,
peripheral artery disease, and chronic kidney disease. Antihypertensive are drugs used to regulate
blood pressure which is a measure of the repulsive force of the blood on the vessel wall. In this study,
investigations of the genotoxic effect of antihypertensive agents using different genotoxicity tests and
model organisms were reviewed and the results were presented. The genotoxic effect of
antihypertensive was evaluated in many in vivo and in vitro studies. Some of these studies showed that
some drug active ingredient did not have genotoxic effect. On the other hand, some other studies have
suggested that long-term use of antihypertensive drugs may be genotoxic and therefore associated with
increased risk of cancer. In addition, the association between hypertension disease and increased
incidence of some types of cancer (especially renal cancer) is determined in some studies. For this
reason, it is considered that the in vitro and in vivo genotoxic effects of antihypertensive should be
investigated by independent laboratories.

KEYWORDS
Antihypertensive, genotoxicity, cancer

Corresponding Author: ECE AVULOLU-YILMAZ, eceavuloglu@hotmail.com

1
GAZ NVERSTES, FEN FAKLTES, BYOLOJ BLM

931
 Poster Session 6
Submission ID: 835

GENOTOXIC AND ANTIGENOTOXIC EFFECTS OF CYNARIN

AGAINST MMC- INDUCED MICRONUCLEUS FORMATION

1 1 1
ESRA ERIKEL , FATMA NAL , DENIZ YZBAIOLU

ABSTRACT
Artichokes are traditionally used as a medicinal plant and consumed fresh or canned since
ancient times. It has been known that artichoke extracts possess many pharmacological activities,
including hepatoprotection, anti-oxidative, anti-inflammatory, anti-microbial, anti-mutagenic, and
anti-proliferative effects. It is a good source of natural phytochemicals such as flavonoids (mainly
apigenin and luteolin) and hydroxycinnamic acids (mono- and dicaffeoylquinic acids i.e.). Cynarin, a
phenolic compound present especially in leaves, is a type of di-caffeoylquinic acid in artichoke. It has
strong antioxidant activity. The aim of this study was to investigate in vitro genotoxic and
antigenotoxic effects of cynarin against MMC induced micronuclei in human lymphocytes (HLs). HLs
from two healthy donors (1 male and 1 female) were incubated with different concentrations of
cynarin (6.25, 12.5, 25, 50, 100 g/mL) alone and simultaneously with mitomycin-C (MMC, 0.20
g/mL) at 37C for 48 h. A negative (sterile distilled water), a solvent (50% methanol) and a positive
control (MMC, 0.20 g/mL) were also run. Cytochalasin B was added at 44 h of culture for blocking
cytokinesis. Totally, 2000 well-spread binucleated cells (1000 binucleated cells per donor) were
examined for each treatment. All the concentrations of Cynarin alone did not induce statistically
significant micronuclei formation compared to controls. Simultaneous treatment of Cynarin+MMC
diminished the frequency of micronuclei induced by MMC alone compared to positive control in all
the concentrations (except 6.25 g/mL). However, this decline was significant at only 12.5 g/mL
concentration. The results of this study showed that cynarin neither genotoxic alone nor strong
antigenotoxic against MMC induced MN formation.

KEYWORDS
Cynarin, genotoxicity, antigenotoxicity, micronucleus

Corresponding Author: ESRA ERKEL, esraerikel@gmail.com

1
GAZ NVERSTES, FEN FAKLTES, BYOLOJ BLM

932
 Poster Session 6
Submission ID: 836

CYTOTOXIC ACTIVITIES OF FRUIT AND LEAF EXTRACTS OF E.

ELATERIUM AGAINST MALIGN MELANOMA

1 2 1 3
FATMA AYDOMU ZTRK , KERIMAN GNAYDIN , MEHMET ZTRK , MUHAMMAD IQBAL CHOUDHARY

ABSTRACT
Ecballium elaterium, a member of the Cucurbitaceae family, is a poisonous plant. The fruit of
the plant is used in different regions of the world to treat sinusitis traditionally. It was reported that it
exhibits various biological activities such as cytotoxic, antitumor, anti-inflammatory, antihepatotoxic,
purgative, cardiovascular, analgesic and antipyretic. To the best of our knowledge, the studies have
been continuing for searching a cure to skin cancer. Recently, the incidence of mortality from
malignant melanoma of the skin has been rising rapidly in white populations around the world for
many years. It accounts for 75% of skin cancer-related deaths in the world, although the incidence is
4% in all skin cancers. As a continuous of our study, Ecballium elaterium was studied against Malign
Melanoma cell lines (HT 144). In this study, cytotoxic activity, intracellular ROS production,
apoptosis of fruit and leaf extracts of E. elaterium were investigated against malign melanoma cells.
Our results revealed that the extracts exhibited strong cytotoxic activity against HT 144. The extracts
induced cell death through apoptosis was used Annexin V/PI. E. elaterium fruit and leaf methanolic
extracts also promoted the release of reactive oxygen species (ROS). Therefore, it can be concluded
that the cytotoxic activity of E. elaterium extracts induced apoptosis through generation of ROS.

KEYWORDS
Malign melanoma, Ecballium elaterium, cytotoxicity, ROS, apoptosis

Corresponding Author: FATMA AYDOMU ZTRK, ftmaydogmus@gmail.com

1
MULA SITKI KOMAN NVERSTES
2
STANBUL NVERSTES
3
KARACH UNVERSTY

933
 Poster Session 6
Submission ID: 837

DOES LUTEOLIN PROTECTS DNA AGAINST GENOTOXINS?

1 1 1
ESRA ERIKEL , DENIZ YZBAIOLU , FATMA NAL

ABSTRACT
Compounds that can resist or repair genetic damage caused by a chemical mutagen are known
as antigenotoxic agents. Recently, numerous studies have been conducted on the antigenotoxic effects
of phytochemicals. Especially, there has been a growing interest in chemopreventive phytochemicals
to protect and to develop alternative treatment strategies against many chronic diseases such as cancer.
Luteolin, one of the most common flavonoid, exists in medicinal plants and in some vegetables. A
major source of luteolin are celery, green pepper, carrots, olives, and artichokes. It is known that this
flavonoid has many biological activities such anti-oxidant, anti-inflammatory, cardioprotective, anti-
diabetic, anti-allergic, and anti-cancer. The purpose of this research is to review investigations on
genotoxic and antigenotoxic effects of Luteolin. In vivo and in vitro studies using different
genotoxicity tests and model organisms have been compiled to include genotoxic and antigenotoxic
activity of luteolin against DNA damage induced by various genotoxins (mitomycin -C, aflatoxin B1,
and hydrogen peroxide i.e.). Numerous studies have demonstrated that luteolin exhibits protective
effect against genetic damage caused by different type of mutagens in in vitro and in vivo.
Furthermore, epidemiological studies have shown that luteolin has an anticancer activity against lung,
head and neck, prostate, breast, colon, liver, cervical, and skin cancers. Generally, this efficacy of
luteolin is associated with the induction of apoptosis and inhibition of cell proliferation, metastasis and
angiogenesis. Thus, it is believed that luteolin could be a potent chemopreventive agent.

KEYWORDS
Luteolin, genotoxicity, antigenotoxicity, cancer

Corresponding Author: ESRA ERKEL, esraerikel@gmail.com

1
GAZ NVERSTES, FEN FAKLTES, BYOLOJ BLM

934
 Poster Session 6
Submission ID: 838

ISOLATION OF THE CHEMICAL COMPONENTS OF TURMERIC

AND THEIR SEMI-SYNTHETIC MODIFICATIONS

1 1 1
GULAY ZENGIN , MERVE FIRAT , HUSEYIN ZENGIN

ABSTRACT
Turmeric (Curcuma longa) is a perennial herb of the ginger or Zingiberaceae family. It is
cultivated mainly in India, China and South East Asia. The turmeric plant has underground rhizomes,
otherwise known as its root, and is a valuable part of this plant, having culinary and medicinal
properties. Its scientific history is covered by two centuries of research. It was in the year 1815 when
it was first isolated [1] and later in 1870 its crystalline form was presented [2], being identified as 1,
6-heptadiene-3, 5-dione-1,7-bis(4-hydroxy-3-methoxyphenyl) (1E,6E) or diferuloylmethane. Potential
medicinal benefits of turmeric is described in the literature on many occassions, and include anti-
microbial and anti-cancer properties, among many others. The literature describes turmeric as an
orange-yellow crystalline powder, and with respect to its solubility it is considered to be soluble in
ethanol, dimethylsulfoxide, and acetone and insoluble in water and ether [3,4]. The characteristic
colour of turmeric is due to the presence of cucurmin, the principal curcurminoid found in turmeric.
The word curcuminoid has been coined to refer to as the chemical constituents of turmeric and include
mainly curcumin (~70%), demethoxycurcumin (~17%) and bis-demethoxycurcumin (~3%), also
known as curcumin I, II and III, respectively. Curcumin exists as the keto-enol tautomeric forms,
where the keto form predominates in acidic and neutral solutions and enol in alkaline solutions [5].
The aim of this study was to extract and isolate components to be reacted with other compounds, in
order to synthesize semi-synthetic compounds of improved medicinal uses. Typical spectroscopic
techniques, such as, GC-MS, NMR, FT-IR, UV-Vis and Photoluminescence will be used for
characterization purposes and in vitro biological and pharmacology data of these new semi-synthetic
derivatives will be obtained. REFERENCES [1] Vogel, H. A., Pelletier, J. Curcumin-Biological and
Medicinal Properties, J. Pharma., 1815, 2, 50. [2] Daube F. V. Uber Curcumin, den Farbstoff der
Curcumawurzel, Ber. Dtsch. Chem. Ges. 1870, 3, 609-613. [3] Milobedeska, J., Kostanecki, V.,
Lampe, V. Structure of Curcumin, Ber. Dtsch. Chem. Ges., 1910, 43, 2163-2170. [4] Lampe, V.,
Milobedeska, J. Studien ber Curcumin, Ber. Dtsch. Chem. Ges., 1913, 46, 2235-2240. [5]
Priyadarsini, K. I. Photophysics, Photochemistry and Photobiology of Curcumin: Studies from
Organic Solutions, Bio-Mimetics and Living Cells, J. Photochem. Photobiol. C: Photochem. Rev.,
2009, 10, 81-96.

KEYWORDS
turmeric, curcumin, anti-microbial properties, photoluminescence

Corresponding Author: MERVE FRAT, firatmerve0777@gmail.com

1
DEPARTMENT OF CHEMSTRY, FACULTY OF SCENCE AND LTERATURE, GAZANTEP UNVERSTY,
GAZANTEP, 27310

935
 Poster Session 6
Submission ID: 839

IDENTIFICATION OF THE COMPONENTS OF WALNUT KERNELS

AND THEIR SOAP PREPARATION

1 1 1
HUSEYIN ZENGIN , OZDEN DOGAN , GULAY ZENGIN

ABSTRACT
Walnuts are the nuts of temperate regions and belong to the Juglandaceae plant family. They
are considered to be the oldest cultivated fruit in the world [1]. Walnuts and grow on large trees and
bear edible nuts described as kernels; the most outer part a tough leathery husk, then the hard shell
enclosing these kernels. A diet intake of these flavorable nuts provides beneficial effects on the human
health, such as the lowering of blood cholesterol [2] and cardiovascular protection [3]. There many
varieties of walnuts but the main types are the Persian or English walnut (Juglans regia L.), Black
walnut (Juglans nigra L.) and White walnut (Juglans cinerea L.). Walnut kernels are rich in oil content,
varing from 52-70%, where cultivar, location and irrigation rate affects this amount [4]. Hence, due to
the high fat content, walnuts may assist in skin treaments for the care and repair of skin functions and
thus provide valuable cosmetic value. The literature provides a wide variety of other medicinal
applications of walnuts and include anti-cancer [5], and anti-microbial properties [6]. The goal of the
study was to extract, isolate and identify the components of walnut kernels. Extraction was to be
ahieved by the soxhlet extraction technique. The extracts were then to be tested for their antimicrobial
effects. The collected walnut kernel oils will be utilized for soap making purposes. REFERENCES [1]
Caglarirmak, N. Biochemical and Physical Properties of Some Walnut Genotypes (Juglans regia L.),
Nahrung, 2003, 47(1), 28-32. [2] Savage, G. P. Chemical composition of walnuts (Juglans regia L.)
grown in New Zealand, Plant Food Hum. Nutr., 2001, 56(1), 75-82. [3] Simopoulos A. P. Essential
Fatty Acids in Health and Chronic Disease. Am. J. Clin. Nutr. 1999, 70(3), 560-569 (Suppl.). [4]
Ozkan, G., Koyuncu, M. A. Physical and Chemical Composition of Some Walnut (Juglan regia L.)
Genotype Grown in Turkey, Grasas Aceites, 2005, 56(2), 142-146. [5] Hardman, W. E. , Ion, G.
Suppression of Implanted MDA-MB 231 Human Breast Cancer Growth in Nude Mice by Dietary
Walnut, Nutr. Cancer, 2008, 60(5), 666-674. [6] Noumi, E., Snoussi, M., Hajlaoui, H., Valentin, E.,
Bakhrouf, A. Antifungal Properties of Salvadora Persica and Juglans regia L. Extracts Against Oral
Candida Strains, Eur. J. Clin. Microbiol. Infect. Dis., 2010, 29(1), 81-88.

KEYWORDS
walnut kernel, oil, anti-microbial effects, soap

Corresponding Author: OZDEN DOGAN, ozdnndognn@gmail.com

1
DEPARTMENT OF CHEMSTRY, FACULTY OF SCENCE AND LTERATURE, GAZANTEP UNVERSTY,
GAZANTEP, 27310

936
 Poster Session 6
Submission ID: 840

DETERMINATION OF THE COMPONENTS OF CRANBERRY SEEDS

AND THEIR ANTI-MICROBIAL EFFECTS AND SOAP PREPARATION
FROM CRANBERRY SEED OIL

1 1 1
HUSEYIN ZENGIN , ADEM MERT , GULAY ZENGIN

ABSTRACT
Cranberries are evergreen dwarf shrubs found worldwide, and fall under the genus Vaccinium,
and their subgenii include oxycoccos, erythrocarpum, macrocarpon and microcarpum, depending, on
the region of cultivation. Cranberry fruit is a berry of admirable sweet sour taste. They have health
promoting qualites and may contribute colour, flavour, and nutritional value, allowing them to be used
as dyes, foodstuff and dietary supplements. They are well known for their rich phenolic compound
content [1], and have been shown to exhibit beneficial anti-oxidant and anti-proliferative properties
[2]. Research on the health benefits of cranberries has been provided in the literature on numerous
occassions [3,4]. The literature gives a report on the anti-adhesion effects of cranberries, leading to
bioactivitiy against urinary tract infections [5]. Anti-adhesion effects were also observed in another
study, offering promise in the prevention and cure of ulcers due to Helicobacter pylori infections [6].
Cranberry seeds are described as being the waste material of cranberry fruit. However, the oils
extracted from these seeds are known for their fine quality and invaluable cosmeceutical applications.
Cranberry seed oil (CSO) is rich in tocotrienols and tocopherols (Vitamin E), and other anti-oxidants,
and has significant amounts of Vitamin A [7], and thus can help nourish, soothe and moisturize skin
and hair. The purpose of the study was to isolate and identify the components of CSO. Several CSO
extraction procedures will be presented and compared for optimal oil yields. The CSO extracts will be
evaluated for their anti-microbial properties, and the extracts collected will be utilized for CSO soap
preparations. REFERENCES [1] Singh, A. P., Wilson, T., Kalk, A. J., Cheong, J., Vorsa, N. Isolation
of Specific Cranberry Flavonoids for Biological Activity Assessment, Food Chem., 2009, 116, 963-
968. [2] Sun, J., Chu, Y. F., Wu, X. Z., Liu, R. H., Antioxidant and Anti Proliferative Activities of
Common Fruits, J. Agric. Food Chem., 2002, 50, 7449-7454. [3] Hakkinen, S., Heinonen, M.,
Karenlampi, S., Mykkanen, H., Ruuskanen, J., Torronen, R. Screening of Selected Flavonoids and
Phenolic Acids in 19 Berries, Food Res. Int., 1999, 32 345-353. [4] Cote, J., Caillet, S., Doyon, G.,
Sylvain, J. F., Lacroix, M. Bioactive Compounds in Cranberries and their Biological Properties, Crit.
Rev. Food Sci., 2010, 50, 666-679. [5] Howell, A. B. Cranberry Proanthocyanidins and the
Maintenance of Urinary Tract Health, Crit. Rev. Food Sci. Nutr., 2002, 42, 273-278. [6] Burger, O.,
Weiss, E. I., Sharon, N., Tabak, M., Neeman, I., Ofek, I. Inhibition of Helicobacter Pylori Adhesion to
Human Gastric Mucus by a High-Molecular-Weight Constituent of Cranberry Juice, Crit. Rev. Food
Sci. Nutr., 2002, 42 (Suppl.), 279-284. [7] Nawar, W. W. Tocotrienols and Omega-3 Fatty Acids in
Cranberry Seed Oil, FASEB J., 2001, 15(5), A985-A985.

Corresponding Author: ADEM MERT, ademmert@gantep.edu.tr

1
DEPARTMENT OF CHEMSTRY, FACULTY OF SCENCE AND LTERATURE, GAZANTEP UNVERSTY,
GAZANTEP, 27310

937
KEYWORDS
cranberry fruit, cranberry seed oil, anti-microbial effects, soap

938
 Poster Session 6
Submission ID: 841

SYNTHESIS, CHARACTERIZATION AND ANTI-MICROBIAL

STUDIES OF DOPAMINE-DERIVED SULFONAMIDES

1 2 1
GULAY ZENGIN , MUDHAFAR SALLOOM AHMED AL TAMEEMI , HUSEYIN ZENGIN

ABSTRACT
Dopamine (DA) is a catecholamine neurochemical having neurohormonal functions that
include cognition, and coordination. In addition to being present in the human body, it is found in
many types of food. DA is essential for the proper life-dependent functions of the human body, and
plays an important role in many diseases and disorders, of which Parkinsons disease [1] and
schizophrenia [2] are the most common ill conditions. Further, DA has also been shown to have anti-
oxidant properties [3], effectively scavengering any superoxide and hydroxyl radicals (O2- and HO-,
respectively), and thus has been widely used in the food industry, as well as for medicines. The
literature provides work on DA and various derivatives of pharmaceutical applications, and as anti-
oxidants and anti-microbial agents in the food and cosmetic industry. Sulfonamide-type drugs are
known for their varied biological applications, and are particularly used for the treatment of urinary
tract infections, bronchitis and malaria [4]. The aim of this study was to synthesize new dopamine-
sulfonamide derivatives with valuable medicinal properties, for potential use in the treatment of
various ill states and disorders, and for application in food and cosmetics. The characterization
techniques to be used include FT-IR, UV-Vis, GC-MS, NMR and Photoluminescence. Further, in-
vitro biological and pharmacology data for the synthesized novel dopamine-sulfonamide derivatives
will also be obtained. REFERENCES [1] Mueller, T. Catechol-O-Methyltransferase Inhibitors in
Parkinson's Disease, Drugs, 2015, 75, 157-174. [2] Howes, O., McCutcheon, R., Stone, J. Glutamate
and Dopamine in Schizophrenia: An Update for the 21st Century, J. Psychopharmacol., 2015, 29(2),
97-115. [3] Yen G. C., Hsieh C. L. Antioxidant Effects of Dopamine and Related Compounds, Biosci.
Biotechnol. Biochem., 1997, 61, 1646-1649. [4] Hansch C., Sammes P. G., Taylor J. B.
Comprehensive Medicinal Chemistry, Vol. 2, Pergamon Press, Oxford, UK, 1990.

KEYWORDS
dopamine, sulfonamide, anti-microbial properties, photoluminescence

Corresponding Author: MUDHAFAR SALLOOM AHMED AL TAMEEM, mudhafarahmed76@gmail.com

1
DEPARTMENT OF CHEMSTRY, FACULTY OF SCENCE AND LTERATURE, GAZANTEP UNVERSTY,
GAZANTEP, 27310
2
BOCHEMCAL SCENCE AND TECHNOLOGY, SCHOOL OF NATURAL AND APPLED SCENCE, GAZANTEP
UNVERSTY, GAZANTEP, 27310

939
 Poster Session 6
Submission ID: 842

EFFECTIVENESS OF GARLIC ON HIGH BLOOD PRESSURE

1 1
ALAR AKALI , ASLI UAR

ABSTRACT
Death rates due to cardiovascular diseases is the first in the world and in our country. Recently
it has been reported that garlic has lowered blood pressure and therefore has a positive effect in
preventing cardiovascular diseases. This effect was associated with the alicin compound found in
garlic. While this compound regulates the blood pressure with the help of angiotensin-II inhibition and
vasodilator effect, its mechanism of action is still contradictive. It has been stated that garlic and allisin
that is its active metabolite cause a nitric oxide dependent relaxation on pulmonic arteries that are
isolated from rats. It has been asserted that gamma-glutamylS-allyl cysteine which is a peptide in
garlic inhibits an enzyme that takes a role in production of certain hormones increasing blood pressure.
Moreover, it has been stated that garlic regulates the blood pressure of hypotensive people. Garlic is
the second most common consuming food among individuals with cardiovascular diseases due to
these effects. It is used in many European countries such as Germany, England and Austria due to its
nutraceutical properties. Some studies suggest that garlic and garlic derived bioactives have important
medicinal features with the potential for ameliorating hypertension. In this study, it was aimed to
investigate the studies which searched garlics effect on blood pressure, and to make recommendations
interested with nutrition.

KEYWORDS
garlic, blood pressure, alicin, nutrition

Corresponding Author: ALAR AKALI, akcalicaglar@gmail.com

1
ANKARA NVERSTES SALIK BLMLER FAKLTES

940
 Poster Session 6
Submission ID: 843

ANTIOXIDANT PROPERTIES AND PHENOLIC COMPONENTS OF

PRUNUS SPINOSA L. BRANCHES, LEAF AND FRUITS FROM
TEKIRDAG

1 2 2 2
GLAH YILMAZ , YAKUP RN , HILAL EBRU AKIR , SEVGI KOLAYLI

ABSTRACT
Prunus spinosa, named in traditional as akal plum, or gvem plum, a species from the family
of Rosaceae. A wide distribution area is shown in Anatolia. This plum type is widely consumed as
fresh, dried or marmalade. The high antioxidant capacity of the fruit is depends on polyphenols
compositions and this changed collected area geographical properties.1 In this study, polyphenolic
profiles and antioxidant capacities of Gvem plum was studied in the three paert of stembark, leaf and
fruit collected from Tekirda Muratl region. The total phenolic contents of the ethanolic extracts was
found 382, 927 and 956 mg GAE / 100 g in fruit, leaf and branch, respectively. Rutin was the major
component. While rutin, epicatechin and ferulic acid were detected in stembarks, vanillic acid,
-OH benzoic acid and p-coumaric acid were found in the fruits. Syringic acid and cinnamic
acid were detected only on the leaf side. Compared with other studies, Gmhane species, vanillic
acid and benzoic acid were major components of the fruit, but it was found that other polyphenols
were changed. As a result, the stembark and leaves of Gvem plum is rich in flavanoids, and it is
beneficial for public health to evaluate these parts as phytotherapy. References 1. Aliyaziciolu, R.,
Yildiz,O., Sahin, H.,Eyuolu, O. E., Ozkan, M.T., Karaolu, S. Kolayl,S. Phenolic Components and
Antioxidant Activity of Prunus spinosa from Gumushane, Turkey. Chemistry of Natural Compounds ,
51, 2 346-349, 2015.

KEYWORDS
Guvem plum, antioxidant, phenolics, flavanolids, Tekirdag

Corresponding Author: GLAH YILMAZ, ylmzgulsah@gmail.com

1
KARADENZ TEKNK NVERSTES BYOLOJ BLM
2
KARADENZ TEKNK NVERSTES KMYA BLM

941
 Poster Session 6
Submission ID: 844

THE EFFECTS OF DIFFERENT PREPARATION CONDITIONS ON

THE ANTIOXIDANT POTENTIALS OF CINNAMON, CLOVE AND
HIBISCUS TEAS

1 1
FATMA GIZEM AKAKAYA , LEVENT YURDAER AYDEMIR

ABSTRACT
In this study, the effects of different preparation conditions on the antioxidant activities of
cinnamon, clove and hibiscus teas were investigated. The teas were prepared by infusion at 95C and
decoction of dried plant materials (2 g/40 ml deionized water) for 5 and 10 minutes. Total phenolic
contents (TPC), free radical scavenging activities (FRSA), and iron chelating capacities (ICC) of teas
were determined and their antioxidant potentials were evaluated by using Folin-Ciocalteu reagent,
measuring the DPPH radical scavenging activity, and measuring the Fe2+ chelating capacity,
respectively. The highest TPC and FRSA values were determined for hibiscus teas and varied from
19580 to 52779 g gallic acid/g and from 54.86 to 73.91 mol trolox/g, respectively. Hibiscus teas had
between 2 and 45 times higher TPC and between 1.25 and 17 times higher FRSA than other tea
samples. As the infusion time increased from 5 to 10 minutes, TPC (between 12 and 87%), FRSA
(between 62 and 93% - except for clove teas) and ICC (more than 500%) values of teas were
significantly increased (P0,05). Particularly cinnamon tea had the highest increment in antioxidant
parameters among teas. On the other hand, decoction process had reverse situation and all parameters
were inversely changed as the time was increased (P0,05). Only ICC of clove tea increased almost 4
times. In hibiscus tea, TFC was conserved while FRSA was decreased for 25% as the decoction time
was increased. However, decoction process produced teas with lower TPCs compared those of
produced with infusion process, FRSA values of teas were higher in 5 minute-decoction but lower in
10-minute-decoction (P0,05). This study revealed the potential of mostly consumed teas to use as
natural antioxidant additive in functional food formulations by determining the variations in their
antioxidant activities depending on the preparation conditions. For better evaluation, further studies
are needed to determine the storage stability of teas and formation of powder from teas by
lyophilization or spray drying.

KEYWORDS
cinnamon, clove, hibiscus, antioxidant activity, functional food

Corresponding Author: LEVENT YURDAER AYDEMR, lyaydemir@adanabtu.edu.tr

1
ADANA BLM VE TEKNOLOJ NVERSTES

942
 Poster Session 6
Submission ID: 845

ANTIOXIDANT ACTIVITIES OF ROSA CANINA SPECIES

COLLECTED FROM TURKEY

1 2 3 4 5 6
MESUT IIK , HSEYIN KANBUR , EMRAH DKC , FATIH TOZOLU , KR BEYDEMR , LHAMI GLN ,
7
EKREM KKSAL

ABSTRACT
Plants generate secondary metabolites, which could be used as novel therapeutic compounds.
These compounds are well known for their beneficial effects on human health. Thus, it is important to
evaluate total phenolic and flavonoid contents as well as antioxidant activities of different plants. The
present study was conducted to determine the total phenolic/flavonoid content and antioxidant activity
of ethanol extract prepared from fruits of Rosa canina. This black Rosa canina is only grown in
Gmhane, Bayburt, Ar and Erzurum in our country. The total phenolic content of the ethanol
extract of the sample were determined using the Folin-Ciocalteu reagent. Antioxidant activity of Rosa
canina was determined using different in vitro experimental models, which include DPPH, FRAP,
CUPRAC and potassium thiocyanate method. Total phenolic and flavonoid contents of Rosa canina
were found to be 153,4 mg GAE/g and 13,8 mg QE /g, respectively. Antioxidant activity of the sample
was comparable to commercial antioxidant standards (BHT, - tocopherol and trolox). The results
showed that Rosa canina has moderate free radical scavenging and reducing capacity. Overall, this
study discovers total phenolic and flavonoid content as well as antioxidant properties of Rosa canina.

KEYWORDS
Antioxidant, Rosa canina, oxidative stress

Corresponding Author: FATH TOZOLU, fatih.tozoglu@adu.edu.tr

1
HARRAN NVERSTES
2
YILDIZ TEKNK NVERSTES
3
AKSARAY NVERSTES
4
ADNAN MENDERES NVERSTES
5
ANADOLU NVERSTES
6
ATATRK NVERTES
7
ERZNCAN NVERSTES

943
 Poster Session 6
Submission ID: 846

POLLEN AND NUTLET MORPHOLOGY OF TWO MEDICINAL

SALVIA L. (LAMIACEAE) TAXA IN HATAY

1 1 1
AHMET LM , BERNA DEMR , FARUK KARAHAN

ABSTRACT
The genus Salvia L. (Lamiaceae), is represented by 950-100 taxa worldwide. The genus has
been widely distributed in five regions of the world; central and South America (*500 spp.), western
Asia (*200 spp.), eastern Asia (*100 spp.), Africa (*60spp.) and Europe (*36 spp.). Turkey is one of
the centres of diversity regions in Southwest Asia with 99 Salvia species. Turkey is main center of
diversity for the genus. Of the 97 Salvia taxa in Turkey, 51 are endemic to the country and endemism
rate of the genus is 52,5%. In this study, pollen and seed morphology of Salvia sericeo-tomentosa var.
tomentosa L. and Salvia sericeo-tomentosa var. hatayica L. endemic for Turkey were investigated.
Plants specimens used in this study were collected from evlik coastal area (C6 Hatay) in vegetation
period in 2016. The detailed pollen and seed morphological structures of Salvia sericeo-tomentosa var.
tomentosa L. and sericeo-tomentosa var. hatayica L. were comparatively studied using scanning
electron microscopy (SEM). In the SEM investigations pollen grains were directly placed onto stubs,
sputter-coated with gold, and examined by ZEISS Evo LS10 scanning electron microscope.
Palynological analysis showed that the polen shape of S. sericeo-tomentosa var. hatayica is oblate, but
S. sericeo-tomentosa var. tomentosa is suboblate. The ornamentasyon are reticulate and bireticulate.
Aperture number/type is similar (6-colpus). The nutlets are subprolate and prolate-spheroidal. nutlet
surface sculpturing are reticulate. However according to size of nutlets, Salvia sericeo-tomentosa var.
hatayica (1,58x,66 mm) is bigger than S. sericeo-tomentosa var. tomentosa (1,28x1,62 mm). The
results demonstrated that pollen and seed micromorphology useful for the identification of these
species. We believe that fnding of this study will significantly contribute to the biodiversity and
taxonomy studies of medicinal endemic plant species at local and regional scales.

KEYWORDS
Lamiaceae, Salvia, Pollen morphology, Seed morphology, SEM.

Corresponding Author: BERNA DEMR, bernademir1067@gmail.com

1
MUSTAFA KEMAL NVERSTES BYOLOJ BLM

944
 Poster Session 6
Submission ID: 847

ANTIOXIDANT PROPERTIES OF ETHANOLIC EXTRACT OF BEE

POLLEN FROM ERELI (KONYA, TURKEY) REGION

1 1 1
GKHAN ZENGN , HALUK OZPARLAK , RAMAZAN CEYLAN

ABSTRACT
Pollen of bee is one of the purest and the richest natural food supplements packed by
honeybees into granules and subsequently harvested from hives by humans. Honeybee-collected
pollen is composed of nutritionally essential substances such as proteins, amino acids, carbohydrates,
lipids, vitamins, mineral substances and trace elements. Its beneficial effect on health is thought to be
due to the presence of phenolic compounds with its antioxidant activity. The aim of this study was to
determine the total phenolic and flavonoid contents and antioxidant activity of ethanolic extract of
honeybee-collected pollen from Ereli (Konya, Turkey) region for the first time. In this study,
ultrasonication assisted extraction method was used in contrast to routine methods. Total phenolic and
flavonoid contents present in the extract were also determined by Folin-Ciocalteu and AlCl3 assays.
Antioxidant activities were investigated by using different assays, including free radical scavenging
assays (DPPH and ABTS) reducing power (FRAP and CUPRAC), phosphomolybdenum and metal
chelating assays. Total phenolic and flavonoid contents were found to be 15.90 mgGAE/g extract and
4.89 mgRE/g extract, respectively. The extract was more effective than ABTS (34.77 mgTE/g extract)
as compared to DPPH (19.64 mgTE/g extract). Moreover, the reducing abilities were 77.12 mgTE/g
extract for CUPRAC and 18.02 mgTE/g extract for FRAP. Molybdenum reducing power was found to
be 260.54 mgTE/g extract. Ferrous chelating power was 9.47 mgEDTA/g extract. Results indicate
that, pollen of bee from Ereli region could be considered as a natural source of high-valued functional
ingredients for further use in healthful formulations.

KEYWORDS
Bee pollen, Ereli, Free radical scavenging, Konya, Total flavonoid, Total phenolic.

Corresponding Author: HALUK OZPARLAK, hozparlak@selcuk.edu.tr

1
SELCUK UNVERSTY, SCENCE FACULTY, DEPARTMENT OF BOLOGY, KONYA, TURKEY

945
 Poster Session 6
Submission ID: 848

IN VITRO ENZYME INHIBITORY PROPERTIES OF ETHANOLIC

EXTRACT OF BEE POLLEN FROM ERELI (KONYA, TURKEY)
REGION

1 1 1
GOKHAN ZENGIN , HALUK OZPARLAK , RAMAZAN CEYLAN

ABSTRACT
Bee pollen is a valuable apitherapeutic product greatly appreciated by the natural medicine
because of its potential nutritional and medical applications. In the last three decades many papers
have been published on issues concerning bee pollen. Although many studies have been conducted on
bee pollen and other bee products, there are few studies of enzyme inhibition. Therefore, the enzyme
inhibitory potentials of ethanolic extract of honeybee-collected pollen from Ereli (Konya, Turkey)
region were investigated against cholinesterase, amylase and glucosidase for the first time. In this
study, ultrasonication assisted extraction method was used in contrast to routine methods. The in vitro
enzyme inhibitory potentials were measured with a microplate reader. The activities were evaluated as
standard equivalents. The extract was found to be effective on these enzymes. Acetylcholine- and
butrylcholinesterase inhibitory activities were 2.51 mgGALAE/g and 1.70 mgGALAE/g, respectively.
Anti-diabetic activity was evaluated with -amylase and -glucosidase inhibitions and the results were
determined as 0.34 mmolACAE/g and 2.57 mmolACAE/g extract, respectively. The results suggested
that honeybee-collected pollen from Ereli region could be considered as a source of natural enzyme
inhibitors for the treatment of major health problems such as Alzheimer Disease and Diabetes
mellitus.

KEYWORDS
Bee pollen, Enzyme inhibition, Ereli, Konya, Natural agents.

Corresponding Author: HALUK OZPARLAK, hozparlak@selcuk.edu.tr

1
SELCUK UNVERSTY, SCENCE FACULTY, DEPARTMENT OF BOLOGY, KONYA, TURKEY

946
 Poster Session 6
Submission ID: 849

THE CHARACTERISTICS OF POPLAR MUSHROOM (AGROCYBE

AEGERITA) AND WOOD CHICKEN MUSHROOM (LEATIPORUS
SULPHUREUS) SPECIES

1
EYUP BAER

ABSTRACT
In this study, some characteristics of the species of poplar mushroom (Agrocybe aergerita) and
wood chicken mushroom (Leatiporus sulphureus) identified in Konya ecology have been discussed.
The poplar mushroom was found in the root zone of poplar tree and the wood chicken mushroom was
found on trunk of the black willow tree (Salix nigra). Poplar mushroom is a kind of mushroom which
is on the white stalk with a cap and milky coffee color, non-poisonous, edible and delicious. If poplar
mushroom spores are present in the roots or logs of old poplar trees, they grow spontaneously when
ambient conditions occur. Mushrooms can harvest in 20-28 days, if mushroom spores begin to develop
after rainfall in spring and autumn, when the relative humidity reaches 95-100% and the temperature
reaches 21-27 0C in Konya central ecology. Poplar mushroom is a natural type of Turkey nature and is
not culturally cultivated but it is seen in the nature of Asia, Europe and Australia and culture is also
being made. The wood chicken mushroom known as sulfur mushroom is a kind of edible mushroom
species from the Polyporaceae family. The taste is known as chicken mushroom because it is similar
to chicken meat taste. Chickens are easily recognized by their large clusters of overlapping brackets,
and bright yellow-orangish colors. The colors fade as the mushroom grows older. Its appearance is
impressive, like an opened large flower that in fleshy, in large chunks, and weight can be too much.
The chicken mushroom tree which is the subject of my work of this study, have been identified in the
fall on the ordinary willow tree (Salix nigra). It has been reported in the literature in which chicken
mushrooms are also found in eucalyptus, elm and walnut trees. If the chicken mushroom is not
harvested on the tree, it becomes aged and becomes wood. Fresh mushrooms should be preferred for
consumption. Wood chicken mushrooms can cause allergies in some people. Adults should consume a
small amount by trying. It has been reported that wood chicken mushrooms cause some problems such
as hallucinations and ataxia in children and that consumption is not appropriate. As a result, the types
of poplar and wood chicken mushrooms found in the central ecology of Konya are not among the
cultivated mushroom species that are very well known in Turkey. It has been concluded that it is
appropriate to study for cultivation these mushroom species in terms of human nutrition and economic
contribution.

KEYWORDS
poplar mushroom, Agrocybe aegerita, wood chicken mushroom, Leatiporus sulphureus

Corresponding Author: EYUP BAER, ebaser32@gmail.com

1
BAHR DADA ULUSLARARASI TARIMSAL ARATIRMA ENSTTS -KONYA

947
 Poster Session 6
Submission ID: 850

CULTIVATION OF NATURAL PLANTS "MEDICAL MINT"

EXAMPLE IN TOKAT-ERBAA

1 2
TUBA AHN , CEVDET YILMAZ

ABSTRACT
The rural part of Tokat's Erbaa County is very rich in terms of medical and aromatic plant
diversity, and some people continue their livelihood by collecting them. Some of these plants that
grow in natural environment are cultured depending on demand. As a matter of fact, some plants such
as rosehip, poppy, fenugreek grass are raised by taking incentives from the government. One of these
plants, Medical Mint (Mentha x piperita L.), has grown in size over the last years in terms of the size
of the cultivation area, as well as the amount of production. In the research, the subjects such as the
production, drying, processing, packaging, marketing and exporting of the medical mint in the rural
part of Erbaa have been determined on site by contacting the relevant institutions. The research also
examines the place of other plants in natural plant diversity of Erbaa and the potential and emphasizes
its contribution of Erbaa and Turkey to the economy in case of making use of this potential. Some of
the farmers who participated in the medical and aromatic plant course launched in Erbaa in 2012
applied for the benefit of the relevant monetary grant and incentive for production after they have
received their certificates. The mint seedlings brought to Erbaa through a company were replicated by
two farmers in an area of 100-150 m and first production was achieved. Thus, the medical mint whose
production started in 2013, soon attracted attention with its efficiency in rural part of Erbaa due to the
suitability its geographical conditions. Later, as a result of the fact that purchase guarantee was
provided for this plant whose production continued with contracted agriculture method to the
producers, they achieved a guaranteed and stable income. The harvest that starts on May, despite
changing based on the natural conditions, continues up to November in harvesting 4-5 times. At each
harvest, 2 tons per acre are produced. There is also no need for extra labor due to the fact that the
natural environment conditions are effective and machinery is used during harvest. The harvested
medical mint is dried with natural methods and exported abroad through the related company. There
are around 10 farmers who produces it, as well as the farmers who are currently in the preparation
stage for sowing. With the increasing demand for natural products, studies on medical and aromatic
plants in the world and in Turkey have gained importance. Natural plants and new drugs derived
therefrom are becoming more and more important as an alternative to widely used drugs such as
antibiotics, etc. The increase in the consumption of these plants as raw materials both in the chemical
and pharmaceutical industries has also changed the way these plants are obtained. The production in
the form of harvest in the past is being replaced with plantation-type production over time. As demand
increases in the market, this transition is accelerating. Increasing market value of natural plants has led
to the necessity of readdressing the production and sale of these plants with more scientific methods.
In addition, in order to slow down the immigration process from rural areas to urban areas, which is
one of the biggest problems of our country, the cultivation of these products in the rural areas provides

Corresponding Author: TUBA AHN, tugba.sahin.329@gmail.com

1
ONDOKUZ MAYIS NVERSTES SOSYAL BLMLER ENSTTS
2
ONDOKUZ MAYIS NVERSTES ORTA RETM SOSYAL ALANLAR ETM

948
an important contribution. The revenue generated is likely to be an important revenue channel for
those residing in rural areas and wanting to engage in such agricultural activities. As one of the leading
counties in Turkey, Erbaa contributes to the economy of the country through both rural development
and exports.

KEYWORDS
Medical and Aromatic Plants, Medical Mint, Erbaa, Tokat.

949
 Poster Session 6
Submission ID: 851

INVESTIGATION OF PHENOLIC COMPOUNDS AND ANTIOXIDANT

ACTIVITY OF TEUCRIUM POLIUM L. DECOCTION AND INFUSION

1 1 1 2
ZLEYHA ZER , TURGUT KILI , SEMA ARIKCI , HASIBE YILMAZ

ABSTRACT
Teucrium polium L. belongs to the family of Lamiaceae (Labiatae), which is one of the most
common and diverse plants in the world, comprising over 150 species. T. polium named as mayasl
otu and widely used as herbal tea in folk medicine. Also decoction and infusion of this species is used
as treatment diabetes, kidney, liver diseases, stomach and hemorrhoids. In the previously studies anti-
inflammatory, anti-nociceptive, anti-bacterial and anti-hypertensive activities of T. polium were
reported. In this study, we report the phenolic compounds and antioxidant activity of the decoction and
infusion of T.polium. The quantitative amounts of the phenolic compounds were determined by
LC/MS-MS. The main compounds and amounts were determined as follow for decoction; fumaric
acid, luteolin-7-glucoside, luteolin-5-o-glucoside, palargonin (2060.09; 1167.04; 835.18; 829.96
mg/kg dried herba, respectively). For the infusion samples main compounds and amounts were as
follow; fumaric acid, luteolin-7-glucoside, palargonin, luteolin-5-O-glucoside (1456.2; 431.15; 312.5;
278.43mg/kg dried herba, respectively). The antioxidant activities were determined based on three
methods: 2,2-Diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging, -carotene linoleic acid and
cupric ion reducing antioxidant capacity (CUPRAC) assays. For all the activity assays, infusion and
decoction of the T.polium showed good activity.

KEYWORDS
Teucrium polium, decoction, infusion, phenolic compounds, antioxidant activity.

Corresponding Author: ZLEYHA ZER, zuleyhaozer@balikesir.edu.tr

1
BALIKESR NVERSTES
2
TUBITAK (UME)

950
 Poster Session 6
Submission ID: 854

PHENOLIC COMPOUNDS AND ANTIOXIDANT ACTIVITY OF

SIDERITIS SIPYLEA BOISS. TEA

1 1 1 2
TURGUT KILI , SEMA ARIKI , ZLEYHA ZER , HASIBE YILMAZ

ABSTRACT
The genus Sideritis (Labiatae=Lamiaceae), collected under two sections, 46 species, 12
subspecies and two varieties in Turkey. The species are widely found in the Mediterranean area
especially Spain and Turkey and Turkey is gene center of these species. Moreover Sideritis is one of
the genera with high endemism rate, almost 80%. The species have spread especially in Western
Anatolia. Sideritis species have been popularly used as herbal tea especially in Aegean and
Mediterranean regions for centuries due to their anti-inflammatory, anti-ulcerogenic, digestive and
antimicrobial properties. In this study, we aimed to determine phenolic compounds and antioxidant
activity of infusion and decoction tea samples of the Sideritis sipylea. Fort his purpose, the aerial parts
of S. sipylea collected from Sipil Mountain (Manisa)-Turkey, in June 2014. The quantitative amounts
of the phenolic compounds were determined by using LC/MS-MS. Fumaric acid and chlorogenic acid
were determined as the main components of the both decoction and infusion. The amounts are as
follow; fumaric acid (277.7; 281.05 mg/kg dried herba) and chlorogenic acid (204.47; 174.85 mg/kg
dried herba) respectively. The antioxidant activities were determined based on three methods: 2,2-
Diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging, -carotene linoleic acid and cupric ion
reducing antioxidant capacity (CUPRAC) assays. The tea samples of S. tmolea showed good
antioxidant activity for all the tested activity assays.

KEYWORDS
Sideritis sipylea, phenolic compounds, antioxidant activity, decoction, infusion

Corresponding Author: TURGUT KILI, tkilic@balikesir.edu.tr

1
BALIKESR NVERSTES
2
TBTAK UME

951
 Poster Session 6
Submission ID: 855

PHENOLIC COMPOUNDS AND ANTIOXIDANT ACTIVITY OF

SIDERITIS TMOLEA P. H. DAVIS TEA

1 1 1 2
SEMA ARIKI , TURGUT KILI , ZLEYHA ZER , HASIBE YILMAZ

ABSTRACT
Sideritis L. belongs to the family of Lamiaceae (Labiatae), which is one of the most common
and diverse plants in the world, comprising over 150 species. Sideritis species are generally known
under the names adacayi or dagcayi and widely used as herbal tea in folk medicine in Turkey as
well as Europe. Phenols are very important plant constituents because of their scavenging ability on
free radicals due to their hydroxyl groups. Therefore, phenolic contents of plants may contribute
directly to their antioxidant activity. Sideritis tmolea P.H. Davis, named as Sivri ay in vernacular, is
an endemic species for Turkey. Aerial parts of S. tmolea collected in July 2015 from Bozda, demi
in Turkey. In present study, we investigated the phenolic compounds and antioxidant activity of the
decoction and infusion of S. tmolea. The quantitative amounts of the phenolic compounds were
determined by using LC/MS-MS. The main compounds and amounts were determined as follow for
decoction; fumaric acid, penduletin and chlorogenic acid (240.33; 160.85; 160.21; mg/kg dried herba,
respectively). For the infusion samples main compounds and amounts were as follow; fumaric acid,
chlorogenic acid and penduletin (238.71; 142.22; 70.22 mg/kg dried herba, respectively). The
antioxidant activities were determined based on three methods: 2,2-Diphenyl-1-picrylhydrazyl
(DPPH) free radical scavenging, -carotene linoleic acid and cupric ion reducing antioxidant capacity
(CUPRAC) assays. For all the activity assays, infusion and decoction of the S. tmolea has showed
good activity.

KEYWORDS
Sideritis tmolea, phenolic compounds, antioxidant activity, decoction, infusion

Corresponding Author: SEMA ARIKI, carikci@balikesir.edu.tr

1
BALIKESR NVERSTES
2
TBTAK UME

952
 Poster Session 6
Submission ID: 856

PHENOLIC COMPOUNDS OF CHERRY LAUREL (LAUROCERASUS

OFFICINALIS ROEM.)

1 2 2 3
HILAL AKIR , ZEHRA CAN , SEVGI KOLAYLI , MERYEM KARA

ABSTRACT
Cherry laurel (Laurocerasus officinalis Roem.) or taflan is a characteristic summer fruit of
Blacksea Region. Its a variety of cherry that grows up without using agricultural pesticide or
fertilizer. Its mostly consumed as fresh fruit. Also its consumed after dried, pickled or boiled as
molasses. Total phenolic content, total flavonoid content, antioxidant activity and phenolic
compositions of the edible parts of the fruits were investigated in this study. The quantity of total
phenolic content was found as 280 mg GAE /100 g fresh fruit according to Folin-Ciocalteu method,
total flavonoid content was found as 7.2 mg QUE/100 g and total antioxidant activity was found as

determined by using HPLC-UV system. While the major component was found as vanillic acid;
syringic acid, epicatechin, gallic acid, p-OH benzoic acid, protocatechuic acid, caffeic acid, rutin,
luteolin, p-coumaric acid and ferulic acid were determined in a descending sort. The cherry laurel fruit
that has high phenolic content and antioxidant capacity is a valuable natural fruit for preventive
medicine. It will be beneficiary to extend its utilization by cultivation and to return it to the economy.

KEYWORDS
Cherry laurel, Laurocerasus officinalis Roem., phenolic compounds, antioxidant

Corresponding Author: MERYEM KARA, meryemkaram72@gmail.com

1
KARADENZ TEKNK NVERSTES, FEN FAKLTES, KMYA BLM
2
GRESUN NVERSTES, EBNKARAHSAR TEKNK BLMLER MESLEK YKSEKOKULU,
3
SELUK UNVERSTY CUMRA VOCATONAL HGH SCHOOL

953
 Poster Session 6
Submission ID: 857

RELATION OF MINERAL COMPONENTS IN OUR NUTRITION AND

CANCER

1
YAVUZ YKSEL

ABSTRACT
Cancer is one of the most important diseases of today. There are many types of cancer. Every
type of cancer is affected by many factors. In some societies, the incidence and type of cancer and
their lifestyle, culture and nutritional habits have been compared. Misnutrition is one of the causes of
cancer. Reduction of cancer risk can be achieved through proper nutrition. We take many food items
with our food in our bodies. While some nutrients help prevent cancer formation, some nutrients can
also increase. Minerals, which are a major factor in the survival of our vital functions, are one of these
nutrients, even though they are small in quantity. In our regular work of our body, more than 20 denier
minerals are involved. Some minerals help prevent cancer, while others cause cancers. Some of them
affect both ways, depending on their intake patterns. In this study, information on the types of
minerals found in foods and the positive or negative effects on cancer were compiled.

KEYWORDS
Nutrition, Mineral components, Cancer

Corresponding Author: YAVUZ YKSEL, yavuzyuksel@balikesir.edu.tr

1
BALIKESR NVERSTES

954
 Poster Session 6
Submission ID: 858

THE RESEARCH OF THE USING OF OPUNTIA FICUS-INDICA, A

MEDICAL AROMATIC PLANT IN THE FIELD OF LANDSCAPE
ARCHITECTURE

1 2 3
HEDA BASIRE AKA , LEVENT YAZC , KBRA YAZC

ABSTRACT
The plants which are significant element of landscape arrangement are used to form both
living and livible places. Being the core elements of green places, plants fulfill so many tasks in terms
of functionality, esthetic and ecology. In this regard, xerophilous landscape movement has gradually
become widespread nowaydays and instead of common cultivated plants, the usage of natural plants
has been increased in planting works. Opuntia ficus-indica from opuntia kind, is a plant of cactus
(Cactaceae) family. In Turkish, some different names such as frenk inciri, frenk yemii, dikenli
incir or public saying kaynanadili are used. This plant can be seen in West and South Anatolia.
As it is an edible fruit, it is a means of living for people. In this study, the using of Opuntia ficus-
indica in the area of landscape architecture for ornamental purposes and application (aesthetic and
functional properties) opportunities are evaluated.

KEYWORDS
Opuntia ficus, Landscape, Medicinal plant

Corresponding Author: HEDA BASRE AKA, suhedabasire@hotmail.com

1
BLENT ECEVT NVERSTES, AYCUMA GIDA VE TARIM MESLEK YKSEKOKULU- ZONGULDAK
2
ORTA KARADENZ GET KUAI TARIMSAL ARATIRMA ENSTTS MDRL, TOKAT
3
GAZOSMANPAA NVERSTES ZRAAT FAKLTES,BAHE BTKLER BLM- TOKAT

955
 Poster Session 6
Submission ID: 860

A TOXICOLOGICAL INVESTIGATION OF GENETICALLY

MODIFIED ORGANISMS

1 1
KEVSER YKSEL , GLDEN ZEHRA OMURTAG

ABSTRACT
Genetically modified organisms (GMO) has been a question of debate in science for years.
Production of GMOs is increasing gradually. They take place in numerous fields of our lives and they
benefit us, however they also have risks. Thus society approaches GMOs with suspician. But the gene
technology that takes place in various sectors from agriculture to health, is a new technology and it has
been improving fast. Therefore there are not sufficient scientific data about the subject, such as; long
term studies or human trials to prove those risks. Even so, it is an acknowledged necessity to approach
this process with caution.

KEYWORDS
Foods, genetically modified organisms, toxicology

Corresponding Author: KEVSER YKSEL, kevser.yuksel2@gmail.com

1
STANBUL MEDPOL NVERSTES

956
 Poster Session 6
Submission ID: 861

UTILIZATION OF ESSENTIAL OILS AS BIOPESTICIDES

1 1 1
SEMA DEMR , BANU AKGN , MERTIN HAMZAOLU

ABSTRACT
Essential oils are lipophilic and highly volatile secondary metabolites of plants. They are
extracted from more than 17,000 aromatic plant species commonly belonging to angiospermic families
Lamiaceae, Rutaceae, Myrtaceae, Zingiberaceae and Asteraceae. They have insecticidal, nematicidal,
ovicidal, fungicidal and bactericidal effects against pathogens and pests owing to the active
biochemical compounds. Mode of action of these effects is based on the ability to disrupt the cell wall
and cytoplasmic membrane of bacteria and fungi, leading to lysis and leakage of intracellular. There is
an increasing demand for alternative strategies to control of insect pests for avoiding the negative
effects of the chemical control of pests. From this aspect, biopesticides is one of the best alternative
strategies for eco-friendly and relatively safe pest management. Biopesticides are much more active to
the targeted pests as opposed to synthetic pesticides that have harmful effects to birds, mammalian
species and human. According to the Food and Agriculture Organization (FAO), about 1000 million
metric tons of food is wasted each year because of mycotoxins produced by moulds during storage.
Boswellia carterii essential oil is recommended for fumigation against these moulds and aflatoxin
metabolites. Active packaging that is based on adding active compounds in the packaging material
is one of the most promising innovations for food preservation. In this context, the use of citronella
essential oil in a coating material for carton packages has been published in a study, recently.

KEYWORDS
essential oils, biopesticides, food preservation

Corresponding Author: SEMA DEMR, sema.demir@tarim.gov.tr

1
GIDA VE YEM KONTROL MERKEZ ARATIRMA ENSTT MDRL

957
 Poster Session 6
Submission ID: 862

CLINICAL STUDIES EXAMINING THE EFFECTS OF ALOE VERA IN

SKIN CONDITIONS: A SYSTEMATIC REVIEW

1
ZEHRA GK METIN

ABSTRACT
Background: Aloe vera is a succulent from the Aloe family includes 400 different species, a
tropical plant which is easily grown in hot and dry climates and widely distributed in Asia, Africa and
other tropical areas. The use of Aloe vera is being promoted for a large variety of skin conditions. The
aim of this systematic review was to summarize all skin oriented clinical studies on Aloe vera products
in human subjects. Methods: Extensive literature search were carried out to identify all clinical studies
published between 1991-2017 years on the subject. Data were extracted from PubMed, Medline,
Google Scholar data bases a predefined standardized manner using Aloe vera, clinical trial, psoriasis,
burn, skin condition, wound-healing research terms. All studies published in English were read by
author and data were extracted in a standardized, predefined manner. Results: A total of thirty-seven
studies met inclusion criteria. They were characterized by different study designs including double-
blind placebo-controlled or double blind controlled (n=27), experimental (n=7) and case-reports (n=3).
Total of 3001 patients participated in all studies. The mean age of participants was 42,2 years. The big
majority of studies were performed in Asian countries (45.9%), USA (18.9), and European countries
(18.9%). Duration of intervention varied between two days and nine months. Aloe vera applied orally
or topically once, twice, third or four times throughout the day. Studies focused on dermatitis (n=10),
wound-healing process (n=6), oral-mucosa related problems including stomatitis, radiotherapy-
induced mucositis, and burning mouth syndrome (n=5), lichen planus (n=3), psoriasis (n=3), burn
(n=3), dry skin (n=3) and other skin-conditions involved ultraviolet erythema test, acne vulgaris,
scabies and sulfur mustard exposure (n=4). Dermatitis-related studies on topical administration of
Aloe vera had contradictory findings whether Aloe vera was effective (n=5) or not (n=5). As for oral
mucosa-related problems, studies (n=2) indicated that use of Aloe vera had superior impact on
radiation-related mucositis rather than placebo and was as effective as placebo in remaining studies
(n=3). Studies (n=3) conducted in oral-vulval Lichen Planus reported that Aloe vera gel reduced
erosive and ulcerative lesions and assisted a complete clinical remission. Considering psoriasis studies,
two of those found that placebo was more effective than Aloe vera, while one of them showed Aloe
vera was superior that of placebo. Likely, wound-healing studies founded misleading findings
including beneficial (n=4), harmful (n=1) or no effects (n=1). In addition, Aloe vera showed a
significant effect on second-degree burn (n=1) or no superior effects than placebo in patients with
sunburn or sun exposure (n=2). All studies (n=3) implied that Aloe vera was efficacious for dry-skin
problem. Other skin-related studies (n=4) involving ultraviolet erythema test, acne vulgaris, scabies
and sulfur mustard exposure problems stated promoting results including significant reductions in the
frequency of pruritus, burning sensation, and inflammatory lesions scores. Moreover, almost all
studies (89.1%) reported no side effects associated with use of Aloe vera. Conclusion: In conclusion,
studies outcomes point that Aloe vera is an important aromatherapic plant is generally well tolerated,

Corresponding Author: ZEHRA GK METN, zehragok85@hotmail.com

1
HACETTEPE NVERSTES HEMRELK FAKLTES HASTALIKLARI HEMREL AD

958
as no serious side effects were reported. However, small sample size, limited number of studies, and
lack of knowledge on randomization procedures in included studies need to be considered. Results on
the effectiveness of Aloe vera are contradictory; study analysis reveals the presence of methodological
gaps preventing to reach final conclusions. Therefore, clinical effectiveness of oral and topical Aloe
vera application is need to be evaluated using recent guidelines and well-designed clinical trials.

KEYWORDS
Aloe vera, medical herbal, skin, randomized trial, systematic review

959
 Poster Session 6
Submission ID: 863

INVESTIGATION OF THE EFFECT OF DIFFERENT DRYING

METHODS ON BIOACTIVITY AND BIOAVAILABILITY OF
CHESTNUT POLLEN

1 1 2
SELMA KAYACAN , HATICE BEKIROLU , OSMAN SADI

D
ABSTRACT

TE
Pollen is a bioactive food with high nutritional value, which is gathered from flowers by
honey bees. Although our country is a very rich country in terms of bee products, there is no
standardization about bee products to put on market as qualified products in some countries. Due to
high water content and nutritional value, pollen can be exposed to spoilage by microorganisms.

EN
Therefore, drying of pollen and preserving the bioactive compounds of pollen when it is dried is
important. In this study, chestnut pollen obtained from a beekeeper in Duzce was dried using oven,
vacuum and microwave dryers and the effect of drying processes on bioactive properties and
bioavailability of chestnut pollen were investigated. To determine the bioactive properties of bee
pollen; total phenolic content, total flavonoid content and total antioxidant capacity were performed.
ES
Total antioxidant capacity was carried out two different methods which were DPPH and CUPRAC
methods. Total phenolic content and total flavonoid content were determined as
spectrophotometrically. According to results of analyses, values of total phenolic content, total
flavonoid content, antioxidant activity and bioavailability of fresh chestnut pollen were decreased after
drying processes. The lowest value of total phenolic content was found with oven method and the
PR

lowest values of total flavonoid and antioxidant activity was found with microwave method. The
bioavailability percentage of the fresh pollen IN fraction was determined to 8.27% for the total
phenolic content. After drying this value was ranged from 2.05 to 3.32%. It was determined that the
total flavonoid content was 1.64%, while it was 1.08 to 1.43% after drying. Total antioxidant activity
decreased from 1.05% to 0.33-0.69%.
T
O

KEYWORDS
Bee pollen, Antioxidant, Phenolic, Flavonoid, Bioavailability
N

Corresponding Author: OSMAN SADI, osagdic@yildiz.edu.tr

1
YILDIZ TEKNK NVERSTES, KMYA METALURJ FAKLTES, GIDA MHENDSL BLM
2
ILDIZ TEKNK NVERSTES, KMYA METALURJ FAKLTES, GIDA MHENDSL BLM

960
 Poster Session 6
Submission ID: 866

CONSOLIDA ORIENTALIS'IN FARKLI EKSTRAKLARININ

ANTIOKSIDAN ZELLIKLERI

1 1 2
GOKHAN ZENGIN , ABDURRAHMAN AKTUMSEK , YAVUZ SELIM CAKMAK

ABSTRACT
In this study, antioxidant effects of different extracts (ethyl acetate, methanol and water) form
Consolida orientalis were investigated with spectrophotometric methods. Antioxidant capacity was
evaluated using different assay including free radical scavenging (DPPH), reducing power (FRAP),
phosphomolybenum and -carotene/linoleic acid test system. Total phenolic and flavonoid contents
were also determined. Total phenolic content in water extract was higher than other extracts. However,
the methanol extract was the richest in terms of flavonoid content. Also, DPPH scavenging activities
were determined as 15.27% (in ethyl acetate), 38.11% (in methanol) and 40.18% (in water) at 1 mg/ml
concentration. The best FRAP activity was observed in the water extract, followed by methanol and
ethyl acetate extracts. In -carotene/linoleic acid test system, the inhibition abilities of these extracts
were ranked as water (86.20%), methanol (76.78%) and ethyl acetate (27.99%). Our findings suggest
that the C. orientalis may serve as an important source of natural antioxidants for using in food and
pharmaceutical industry.

KEYWORDS
Consolida, free radical scavenging, phenolic, different solvents.

Corresponding Author: ABDURRAHMAN AKTUMSEK, aktumsek@selcuk.edu.tr

1
SELCUK UNVERSTY
2
AKSARAY UNVERSTY

961
 Poster Session 6
Submission ID: 867

SPARTIUM JUNCEUM'UN FARKLI EKSTRAKLARININ

ANTIOKSIDAN ETKILERI ZERINE BIR ALIMA

1 1 2
GOKHAN ZENGIN , ABDURRAHMAN AKTUMSEK , YAVUZ SELIM CAKMAK

ABSTRACT
In this study, antioxidant abilities of different extracts (ethyl acetate, methanol and water)
form Spartium junceum were investigated with spectrophotometric methods. Antioxidant capacity was
evaluated using different assay including free radical scavenging (DPPH), reducing power (FRAP),
phosphomolybenum and -carotene/linoleic acid test system. Total phenolic and flavonoid contents
were also determined. Total phenolic and flavonoid contents in methanol extract was higher than other
extracts. Also, DPPH scavenging activities were determined as 32.79% (in ethyl acetate), 77.68% (in
methanol) and 73.65% (in water) at 1 mg/ml concentration. The best FRAP activity was observed in
the methanol extract, followed by water and ethyl acetate extracts. In -carotene/linoleic acid test
system, the inhibition abilities of these extracts were ranked as water (90.86%), methanol (82.38%)
and ethyl acetate (74.82%). From these results, S. junceum could be considered as potential candidate
for designing new nutraceuticals or drugs.

KEYWORDS
Spartium, free radical scavenging, flavonoid, different solvents.

Corresponding Author: ABDURRAHMAN AKTUMSEK, aktumsek@selcuk.edu.tr

1
SELCUK UNVERSTY
2
AKSARAY UNVERSTY

962
 Poster Session 6
Submission ID: 868

ASPHODELINE LIBURNICA KKLERININ METANOL

EKSTRAKTININ ANTIOKSIDAN ZELLIKLERI

1 1
ABDURRAHMAN AKTUMSEK , GOKHAN ZENGIN

ABSTRACT
Asphodeline is one of the most important genera of family Xanthorrhoeaceae (the genus was
recently classified under the family Liliaceae) and widely distributed in the Mediterrenaen region
(mainly in the Middle-East countries). In Turkey this genus contains 20 taxa, 12 of which are endemic.
The genus Asphodeline has medicinal importance and some Asphodeline members are traditionally
used in different countries including Turkey. Antioxidant effects methanol extract form Asphodeline
liburnica were investigated with spectrophotometric methods. Antioxidant capacity were evaluated
using different assay including free radical scavenging (DPPH and ABTS), reducing power (FRAP
and CUPRAC), phosphomolybenum, and metal chelating. Total phenolic and flavonoid contents were
also determined. Total phenolic and flavonoid contents of A. liburnica were determined as 9.67
mgGAE/g extract and 1.48 mgRE/g extract, respectively. Radical scavenging effects in ABTS and
DPPH assays were found to be 66.99 mgTE/g extract and 13.23 mgTE/g extract. Also, the reducing
power activities of the extract were moderate in CUPRAC (33.29 mgTE/g extract) and FRAP (33.81
mgTE/g extract). These findings suggest that the A. liburnica could serve as an important natural
source of biologically active agents for using in food and pharmaceutical industry.

KEYWORDS
Asphodeline, free radical scavenging, phenolic, natural product.

Corresponding Author: GOKHAN ZENGN, gokhanzengin@selcuk.edu.tr

1
SELCUK UNVERSTY

963
 Poster Session 6
Submission ID: 869

ASPHODELINE LIBURNICA KK EKSTRAKTININ ALZHEIMER,

DIYABET VE DERI HASTALIKLARI ILE BALANTILI ENZIM
ZERINE INHIBITR ETKILERI

1 1
ABDURRAHMAN AKTUMSEK , GOKHAN ZENGIN

ABSTRACT
Key enzyme inhibitory theory is the most popular for the management of global health
problems including Alzheimer Disease, diabetes mellitus and skin disorders. For these purposes,
enzyme inhibitory effects (cholinesterase, tyrosinase, -amylase and -glucosidase) of root methanolic
extract form Asphodeline liburnica were investigated with spectrophotometric methods. The
cholinesterase inhibitory activities were determined as 1.67 mgGALAE/g extract in AChE and 1.64
mgGALAE/g extract in BChE. Anti-tyrosinase effect was found to be 29.78 mgKAE/g extract. Anti-
diabetic effects of the extract were determined 0.31 mmolACAE/g extract in amylase and 0.70
mmolACAE/g extract in glucosidase. The presented results suggest that the A. liburnica roots may be
considered as valuable candidate for new nutraceutical, pharmaceutical or cosmeceuticals.

KEYWORDS
Enzyme inhibitory activities, cholinesterase, tyrosinase, amylase, glucosidase.

Corresponding Author: GOKHAN ZENGN, gokhanzengin@selcuk.edu.tr

1
SELCUK UNVERSTY

964
 Poster Session 6
Submission ID: 1844

AROMATHERAPY OILS USED FOR SYMPTOM MANAGEMENT IN

RHEUMATIC DISEASES: A LITERATURE REVIEW

1
AFRA ALIK

ABSTRACT
Background and aim: Rheumatic diseases are characterized by inflammation that affects the
connecting or supporting structures of the body most commonly the joints, but also sometimes the
tendons, ligaments, bones, and muscles. Some rheumatic diseases even affect the organs1,2. Patients
with rheumatologic diseases seek to use complementary therapies due to the etiology of rheumatic
diseases is not fully understood, lack of complete cure, chronic symptoms including pain and fatigue,
decreased quality of life, and side effects related conventional therapies. Particularly, aromatherapy
oils have gained popularity for alleviating rheumatic symptoms in recent years. This review was
written to evaluate the specific effects of aromatherapy oils used for the management of symptoms in
rheumatic disease. METHOD: Extensive literature search was conducted using PubMed database
involving 1990 and 2017 years. Predefined standardized keywords including ankylosing spondylitis,
Behcet disease, fibromyalgia, osteoarthritis, systemic lupus erythematosus and rheumatoid arthritis,
Studies conducted in pediatric population were excluded. A total of 178 articles were found and finally
12 studies met inclusion criteria3-14. RESULTS: Studies had randomized controlled trials (RCTs)
(n=10) and semi-experimental design (n=2). The big majority of studies conducted osteoarthritis
(n=10), in patients with rheumatoid arthritis (n=1) and fibromyalgia (n=1). The total of 727 patients
participated in the studies and the age of patients ranged from 29 to 40 years. Aloe vera (Aloe
Barbadensis), black seed (Nigella sativa), castor oil (Ricinus communis), chamomile (Matricaria
chamomilla), eucalyptus (Eucalyptus globulus), ginger (Zingiber officinale), lavender (Lavandula
angustifolia), lemon (Citrus lemon), orange (Citrus sinesis), peganum (Peganum harmala), peppermint
(Mentha piperita), rosemary (Rosmarinus Officinalis) mostly preferred aromatherapy oils. The
intervention period varied between two and 12 weeks. Aromatherapy oils were applied orally,
topically or with a compress. Specific symptoms including pain, fatigue, joint motion and quality of
sleep were examined in the studies. 3-14. Brief Pain Inventory (BP), Fullerton Advanced Balance
Scale (FAB), Korean Sleep Scale, Modified Health Evaluation Questionnaire (MHEQ), Ontario and
Mc. Master Universities Osteoarthritis Index (WOMAC), SF-36 Quality of Life Scale, Ritchie
Articular Index (RAI) and Visual Analog Score (VAS) often used in data collection process3-14.
Studies testing the effects of ginger oil (n=5), found a significant reduction in pain scores in the
intervention group compared with the control group3-7. Another study examining the impact of
lavender, ginger and chamomile aromatherapy oil blend reported a decrease in pain and fatigue scores
and improved functional capacity8,9. A semi-experimental study conducted in patients with
rheumatoid arthritis, applying black seed topically revealed a significant reduction in disease activity
scores of patients10. CONCLUSIONS: Considering the results of these clinical studies, aromatherapy
oils have promising outcomes in pain, fatigue, inflammation parameters and capability of joint motion.
However, small sample sizes and lack of information on randomization procedures need to be

Corresponding Author: AFRA ALIK, afracalik@hotmail.com

1
HACETTEPE NVERSTES

965
considered. Therefore, well-design RCTs testing the effectiveness of aromatherapy oils on specific
symptoms including morning stiffness, low back pain, or depression are needed. References 1.
Karada A. Romatizmal hastalklarda alternatif tp ve tamamlayc tedavi yntemleri. Uzmanlk Tezi.
Sivas: Cumhuriyet niversitesi Tp Fakltesi, Fiziksel Tp ve Rehabilitasyon Anabilim Dal, 2012. 2.
Gne D. Romatizmal hastalklarn tedavisinde sklkla kullanlan bitkiler ve bitkisel rnler. Bitirme
Tezi. Erciyes niversitesi Eczaclk Fakltesi, Farmakognozi Anabilim Dal, 201. 3. Bliddal H,
Rosetzsky A, Schlichting P, Weidner MS, Andersen LA, Ibfelt HH, Christensen K, Jensen ON,
Barslev J. A randomized, placebo-controlled, cross-over study of ginger extracts and ibuprofen in
osteoarthritis. Osteoarthritis Cartilage. 2000;8(1):9-12. 4. Yip YB, Tam AC. An experimental study on
the effectiveness of massage with aromatic ginger and orange essential oil for moderate-to-severe knee
pain among the elderly in Hong Kong. Complement Ther Med 2008;16(3):131-138. 5. Therkleson T.
Topical ginger treatment with a compress or patch for osteoarthritis symptoms. Journal of Holistic
Nursing 2014;32(3):173-172 6. Paramdeep G. Effcacy and tolerablty of gnger (Zingiber Officinale )
in patients of osteoarthrts of knee. Indian J Physiol Pharmacol 2013; 57(2) : 177183. 7. Drozdov
VN, Kim VA, Tkachenko EV, Varvanina GG. Influence of a specific ginger combination on
gastropathy conditions in patients with osteoarthritis of the knee or Hip. J Altern Complement Med.
2012;18(6):583-588. 8. Kim IJ, Kim EK. Effects of aroma massage on pain, activities of daily living
and fatigue in patients with knee osteoarthritis. Journal of Muscle and Joint Health 2009;16(2):145-
153. 9. Nasiri A, Mahmodi MA,Nobakht Z. Effect of aromatherapy massage with lavender essential
oil on pain in patients with osteoarthritis of the knee: A Randomized Controlled Clinical Trial.
Complementary Therapies in Clinical Practice 2016;25: 7580. 10. Gheita TA, Kenawy SA.
Effectiveness of nigella sativa oil in the management of rheumatoid arthritis patients: A placebo
controlled study. Phytother Res. 2012;26(8):1246-1248. 11. Shoara R, Hashempur MH, Ashraf A,
Salehi A, Dehshahri S, Habibagahi Z. Efficacy and safety of topical matricaria chamomilla L.
(Chamomile) oil for knee osteoarthritis: A randomized nontrolled clinical trial. Osteoarthritis
Cartilage. 2000;8(1):9-12. 12. Abolhassanzadeh Z, Aflaki E, Yousefi G, Mohagheghzadeh A.
Randomized clinical trial of peganum oil for knee osteoarthritis. J Evid Based Complementary Altern
Med 2015;20(2):126-131. 13. Medhi B, Kishore K, Singh U, Seth SD. Comparative clinical trial of
castor oil and diclofenac sodium in patients with osteoarthritis. Phytother Res. 2009;23(10): 1469-
1473. 14. Rutledge DN, Jones CN. Effects of topical essential oil on exercise volume after a 12-week
exercise program for women with fibromyalgia: A pilot study. Journal Of Alternative And
Complementary Medicine 2007;13(10):1099-1106.

KEYWORDS
aromatherapy, herbal medicine, inflammation, rheumatology, symptom control

966
 Poster Session 7
Submission ID: 130

ANALYSIS OF BAY (LAURUS NOBILIS) LEAF VOLATILE OIL BY

GC/MS AND ITS USAGE IN COSMETIC APPLICATIONS

1 1 1
EYDA KIVRAK , TOLGA GKTRK , BRAHIM KIVRAK

ABSTRACT
Bay-tree (Laurus nobilis) is an evergreen tree specie unique to the Mediterranean region and
has been extensively cultivated as an ornamental plant in many countries. Turkey is the leader bay leaf
exporter of the World. The reason of great attention on the bay leaf as a medicinal and aromatic plant
is the content of volatile oil in its leaves. Volatile oils have been used since ancient times in order to
make people healthier, change their appearance, protect the body against possible damage by
environment. Volatile oils are used in fragrances, skin products, hair care products and in general
terms to give a pleasant fragrance to the products. These oils, also known as essential oil, etheric oil by
people, can contain terpenic hydrocarbons and their oxygenated derivatives as well as organic acids,
alcohols, phenols and ketones. Volatile oils are found in any organs of the plant as well as in organs
such as secretory follicles, secretory pockets, secretory ducts or secretory cells according to the family.
It has been conclusived that the essential oils are formed by the hydrolysis of glycosides as well as
asserted that its presence in protoplasm of the plant or formed by the decomposition of the resinous
layer of the cell wall. Antioxidant, antimicrobial, analgesic and antiinflammatory properties of bay
(Laurus nobilis) leaf volatile oil have been proven in previous studies. Previous studies have also
reported the use of bay leaf volatile oil in the form of soap and anti-dandruff hair care preparations. In
this study, the components of the obtained volatile oil were determined using Agilent 6890 GC (Gas
Chromatography) and 5975 MS (Mass Spectrometry). 1,8-Cineole (46.16%), alpha-Terpinyl acetate
(10.62%), alpha-Pinene (6.27%), Terpinen-4-ol (5.07%) and Sabinene (4.99%) were found to be the
major compounds in volatile oil. The obtained volatile oil was used to prepare skin lotion. For this
purpose, 6 g of lilac-flavored hazelnut oil and 6 g of bay leaf oil were added to 87 g of purified water.
Then it was stirred with magnetic stirrer for 10 minutes. Finally, the formulation is completed by the
addition of the homogenized polymer mixture. The rheological properties of the final product were
determined after all treatments were finished.

KEYWORDS
Laurus nobilis, Volatile oil, 1,8-Cineol, Cosmetic

Corresponding Author: EYDA KIVRAK, skivrak@mu.edu.tr

1
MULA SITKI KOMAN NVERSTES

967
 Poster Session 7
Submission ID: 131

DETERMINATION OF PHENOLIC COMPOSITION OF TILIA

TOMENTOSA FLOWERS USING UPLC-ESI-MS/MS

1 1 1
TOLGA GKTRK , EYDA KIVRAK , BRAHIM KIVRAK

ABSTRACT
As a result of bioactive components found in different parts of plant such as flowers, peel, leaf
and increased interest in natural products, medicinal and aromatic plants have found application fields
such as pharmaceutical, cosmetic and dye industries. Therefore, they have been subjected to numerous
researches . Phenolic compounds, which are secondary metabolites of plants, are one of the main
groups of compounds that provide antiallergic, antiarterogenic, antiinflammatory, antimicrobial,
antioxidant, cardioprotective properties of medicinal and aromatic plants. These broad physiological
effects that they possess lead researchers to examine the phenolic contents of plants. Tilia tomentosa
Moench is one of 45 species belonging to Tiliaceae family, and the usage of flowers in traditional
treatment methods is quite common. Researches on different parts of T. tomentosa showed that the
plant possesses spasmolytic, diuretic and sedative effects due to its flavonoids, essential oil and
mucilage components and has been used to treat disorders such as nervous tension, cough, flu,
migraine. There are no studies on phenolic components of its parts such as leaves, flowers using
UPLC-ESI-MS / MS, etc. until now. In this study, firstly, T. tomentosa flowers were extracted with
hexane and volatile oil fractions were separated from the plant. Distilled water was added to the
remaining flower part at 80 C and subjected to extraction for 15 minutes. The obtained extract was
filtered and dried in a lyophilizer at -70 C. The residue was redissolved in a mixture of
water:methanol (80:20). The solution was analyzed by UPLC-MS / MS (Waters Acquity Ultra
Performance LC, Xevo TQ-S MS-MS) by passing through Macherey-Nagel Chromafil Xtra PTFE-
20/25 0.20m filters. According to the analysis results, 3,4-Dihydroxybenzoic acid (66.82 mg/kg),
Myricetin (29.39 mg/kg), Rutin (21.42 mg/kg), Ferulic acid (12.33 mg/kg) ve 3,4-
Dihydroxybenzaldehyde (10.38 mg/kg) were detected. T. tomentosa flowers have great potential to
usage in industries such as food, medicine and cosmetic due to its rich content of phenolics.

KEYWORDS
Phenolic compunds, 3,4-Dihydroxybenzoic acid, Tilia tomentosa, UPLC-ESI-MS/MS

Corresponding Author: TOLGA GKTRK, tolgagokturk@mu.edu.tr

1
MULA SITKI KOMAN NVERSTES

968
 Poster Session 7
Submission ID: 132

INVESTIGATION OF MYRTUS COMMUNIS LEAF ESSENTIAL OIL

AND SEED FIXED OIL COMPOSITION USING GC-MSD

1 1 1
TOLGA GKTRK , EYDA KIVRAK , BRAHIM KIVRAK

ABSTRACT
Myrtus communis belongs to Myrtaceae family and it is a evergreen plant in the form of
shrub. This plant which grows by itself along the Mediterranean region has been used for its medicinal
and aromatic benefits since ancient times. It is called "hambeles", "mersin" or "murt" in various
regions of Turkey. The essential oil of plants leaves has very rich content and this essential oil is
widely used in food, pharmaceutical and cosmetic industries. It has been determined in various studies
that about 30 components constitute %90 of the myrtle leaf essential oil. However, the essential oil
yield and composition varies according to ecological and geographical conditions. Some of the known
biological activities of leaf essential oil include antioxidant activity and antimutagenic activity,
antimicrobial activity, antibacterial activity and antifungal activity. There is few research on the seeds
of plant but in some studies, it has been reported that the content of seeds fixed oil is rich in linoleic
acid and shows antioxidant activity. In this study, the components of essential oil obtained by M.
communis leaves using hydrodestillation and fixed oil obtained by its seeds using cold press method
analyzed with GC-MSD. According to the analyse result, 1,8-Cineole (21.68%), Alpha-Pinene
(18.02%), Linalol (14.12%), Alpha-Terpinyl Acetate (10.40%) and Myrtenol (8.59%) were detected as
majr compounds in the myrtle leaf essential oil. In the content of fixed oil obrained by its seeds
presences Linoleic acid (77.59%) as the major fatty acid. According to the analyse result toher fatty
acids were determined as Palmitic acid (10.36%), Oleic acid (8.26%), Stearic acid (2.81%), Elaidic
acid (0.91%), Eicosanoic acid (0.05%) and Myristic acid (0.03%).

KEYWORDS
Essential oil, Myrtus communis, 1,8-Cineole, GC-MSD

Corresponding Author: TOLGA GKTRK, tolgagokturk@mu.edu.tr

1
MULA SITKI KOMAN NVERSTES

969
 Poster Session 7
Submission ID: 870

ASPHODELUS AESTIVUS KKLERININ FARKLI

EKSTRAKLARININ INVITRO ANTIOKSIDAN AKTIVITELERI

1 1 1
ABDURRAHMAN AKTUMSEK , GOKHAN ZENGIN , SENGUL UYSAL

ABSTRACT
Asphodelus aestivus is mostly used in the treatment of hemorrhoids, nephritis, and burns in
Turkey. The aim of the study was to test the antioxidant activity of A. aestivus extracts obtained by
extraction with ethyl acetate, dichloromethane, methanol, and water. Antioxidant activity of these
extracts was evaluated by different assays (including ABTS, DPPH, FRAP, CUPRAC,
phosphomolybdenum and metal chelating activity). The higher level of total phenolic content was in
the ethyl acetate and dichloromethane extracts. Generally, the ethyl acetate extract exhibited the best
results in these assays. The highest free radical scavenging activity was observed in ethyl acetate
extract in both ABTS (21.23 mgTE/g) and DPPH (9.12 mgTE/g). Our findings suggest that A.
aestivus could be used as a biologically-active compounds for food and pharmaceutical purposes.

KEYWORDS
Asphodelus aestivus, antioxidant, phenolic content, Turkey.

Corresponding Author: SENGUL UYSAL, sennguluysal@gmail.com

1
SELCUK UNVERSTY

970
 Poster Session 7
Submission ID: 871

ASPHODELUS AESTIVUS KKLERININ FARKLI

EKSTRAKLARININ ANTI-KOLINESTERAZ, ANTI-TIROZINAZ,
ANTI-AMILAZ, ANTI-GLUKOZIDAR VE ANTI-LIPAZ
AKTIVITELERI

1 1 1
ABDURRAHMAN AKTUMSEK , GOKHAN ZENGIN , SENGUL UYSAL

ABSTRACT
Asphodelus aestivus is known by different local names like iri otu and yabani prasa in
Turkey. In the present study, the various solvent extracts (ethyl acetate, dichloromethane, methanol
and water) from Asphodelus aestivus were assessed for in vitro enzyme inhibitory activity against
AChE, BChE, -amylase, -glucosidase and tyrosinase. The methanol extract exhibited the strongest
AChE (1.91 mgGALAE/g extract) and BChE (2.07 mgGALAE/g extract) inhibitory activity. The anti-
diabetic activity of A. aestivus was screened using -amylase, and -glucosidase inhibition assays.
The ethyl acetate extract showed high -amylase (0.75 mmolACAE/g) and -glucosidase (2.97
mmolACAE/g) inhibitory activity. The dichloromethane extract had the highest lipase inhibitory
activity (86.32 mgOE/g). A. aestivus could be effective as natural for treatment of various diseases
like Alzheimers disease, diabetes mellitus, and obesity.

KEYWORDS
Asphodelus aestivus, enzyme inhibitory activity, Turkey

Corresponding Author: SENGUL UYSAL, sennguluysal@gmail.com

1
SELCUK UNVERSTY

971
 Poster Session 7
Submission ID: 873

LOTONONIS GENISTOIDES SOLVENT EKSTRATKLARININ

ANTIOKSIDAN ZELLIKLERI

1 1 1
ABDURRAHMAN AKTUMSEK , RAMAZAN CEYLAN , GOKHAN ZENGIN

ABSTRACT
Antioxidant effects of ethyl acetate, methanol, and aqueous extracts from Lotononis
genistoides (Fabaceae) were investigated with spectrophotometric methods. Antioxidant capacity were
evaluated by using different assay including free radical scavenging (DPPH and ABTS), reducing
power (FRAP and CUPRAC), phosphomolybdenum, and metal chelating experiments. Total phenolic
and flavonoid content was also determined as 30.40-53.94 mg GAEs/g and 29.53-51.15 mg REs/g,
respectively. ABTS and DPPH free radical scavenging activities were ranged from 112.90 to126.10
mgTE/g and 80.76 to 99.27 mgTE/g extract, respectively. The best reducing activity in CUPRAC and
FRAP were observed in ethyl acetate and water extracts, respectively. The highest metal chelating
ability were detected in ethyl acetate extract with 12.31 mgEDTAE/g extract, followed by methanol
and water. This study supports the potential use of L. genistoides for designing new functional food
formulations.

KEYWORDS
Lotononis genistoides, antioxidant properties, natural products.

Corresponding Author: RAMAZAN CEYLAN, biyoram7@gmail.com

1
SELCUK UNVERSTY

972
 Poster Session 7
Submission ID: 874

LOTONONIS GENISTOIDES FARKLI SOLVENT

EKSTRATKLARININ NROPROTEKTIF, ANTI-DIABETIC VE DERI
HASTALIKLARI ZERINE INVITRO BIR ALIMA

1 1 1
ABDURRAHMAN AKTUMSEK , RAMAZAN CEYLAN , GOKHAN ZENGIN

ABSTRACT
Enzyme inhibitory properties of water, methanol and ethyl acetate extracts form Lotononis
genistoides (Fabaceae) were investigated by using colorimetric methods. Enzyme inhibitory effects
were evaluated aganist cholinesterases, tyrosinase, -amylase and -glucosidase. The ethyl acetate
(1.92 mgGALAE/g) and methanol (1.89 mgGALAE/g) extracts have the highest acetyl cholinesterase
inhibitory effects as compared to water extract. The ethyl extract exhibited the strongest anti-
tyrosinase effect with 13.51 mgKAE/g. -amylase and -glucosidase inhibition were found to be 0.11-
1.51 and 11.26-28.14 mmol ACAEs/g. Our findings could provide an important contribution for
Lotononis genistoides different solvent extracts potential uses in pharmaceutical and nutraceutical
fields.

KEYWORDS
Lotononis genistoides, enzyme inhibitory potentials, natural products.

Corresponding Author: RAMAZAN CEYLAN, biyoram7@gmail.com

1
SELCUK UNVERSTY

973
 Poster Session 7
Submission ID: 875

DORYCNIUM PENTAPYLLUM SUBSP. HAUSSKNECHTII'NIN

FARKLI METOTLAR ILE ANTIOKSIDAN ZELLIKLERININ
INCELENMESI

1 1
ABDURRAHMAN AKTUMSEK , ALIME CIFTCI

ABSTRACT
The extraction yield and antioxidant properties of Dorycnium pentapyllum subsp.
haussknechtii have been evaluated by use different extraction methods including maceration, soxhlet,
and ultrasonication-assisted extraction. Antioxidant properties were performed by free radical
scavenging activity (DPPH and ABTS), reducing power activity (FRAP and CUPRAC), metal
chelating activity, and phosphomolybdenum assays. Compared with different extraction methods for
the yields, the soxhlet extraction was more efficient than other methods. Total phenolic content was
varied from 48.33 to 105.12 mgGAE/g. In free radical scavenging assays (ABTS and DPPH), the
methanol extract exhibited more potent radical scavenging activity as compared to ethyl acetate and
water extracts. The water extract showed highest metal chelating activity as compared to ethyl acetate
and methanol extracts. This study demonstrated that Dorycinum pentapyllum subsp. haussknechtii can
be use as a promising source in the fields of food and pharmaceutical.

KEYWORDS
Dorycinum pentapyllum subsp. haussknechtii, antioxidant, extraction methods

Corresponding Author: ALME CFTC, alimee.ciftci@hotmail.com

1
SELCUK UNVERSTY

974
 Poster Session 7
Submission ID: 876

DIANTHUS CALOCEPHALUS 'UN ENZIM INHIBITR

ZELLIKLERI ZERINE BIR ALIMA

1 1
ABDURRAHMAN AKTUMSEK , HAMIYET UNUVAR

ABSTRACT
Dianthus genus is represented by 67 species in Turkey. Dianthus species are widely used for
treating gastro-intestinal disorder, wound, and cough. This work aimed to evaluated the effects of
three various solvents (ethyl acetate, methanol, and water) and three different extraction methods
(maceration, soxhlet, and ultrasonication-assisted) on extraction yield, phytochemical profile and
enzyme inhibitory activity of Dianthus calocephalus. The inhibitory activities of extracts were tested
against cholinesterases (AChE and BChE), -amylase, -glucosidase and tyrosinase. The highest
extract yields were obtained from soxhlet extraction method. In all extraction methods, methanol
extract had the highest total phenolic and flavonoid content. The water extract demonstrated the lowest
AChE inhibitory activity than ethyl acetate and methanol. The results showed that ethyl acetate extract
exhibited the greast tyrosinase and -amylase inhibitory activity. D. calocephalus can be used as
natural sources in cosmetic and pharmaceutical industries.

KEYWORDS
Dianthus, extraction methods, enzyme inhibitory activity.

Corresponding Author: HAMYET UNUVAR, hamiyetunuvar91@hotmail.com

1
SELCUK UNVERSTY

975
 Poster Session 7
Submission ID: 877

BARSSIA SP. NOVA (ASCOMYCOTA, PEZIZALES); A NEW

HYPOGEOUS SPECIES TURKEY (OSMANIYE)

1 2
HASAN HSEYIN DOAN , ABAN GNER

ABSTRACT
Osmaniye situated in the East Mediterranean region of Turkey and in square C6 according to
grid square system of Davis (Davis, 1965). Antakya in south Kahramanmara in north, Gaziantep in
east and Adana in west of Osmaniye are located. Many studies on the fungal diversity of Turkey were
investigated by different researchers (Sesli and Denchev, 2014). Recently, new fungal records for
Turkey were determined with macrofungal studies (All et al. 2011; Gngr et al., 2013; Sesli and
Helfer, 2013; Kaya, 2015). Thus, aforementioned these local studies show that it is necessary more
research on fungal diversity of Turkey. The aim of this study is contribute to macrofungi of Turkey
with a new fungal record.

KEYWORDS
Osmaniye situated in the East Mediterranean region of Turkey

Corresponding Author: ABAN GNER, sabanguneri@ogm.gov.tr

1
SELUK NVERSTES, FEN FAKLTES, BYOLOJ BLM
2
ADANA ORMAN BLGE MDRL, ODUN DII RN VE HZMETLER UBE MDRL

976
 Poster Session 7
Submission ID: 878

THE EFFECTS OF LYCIUM BARBARUM (GOJI BERRY)

POLYSACCHARIDES ON 17--ESTRADIOL SERUM LEVELS OF
OVARIECTOMIZED FEMALE RATS

1 1 1 1
BIHTER GKE BOZAT , FATMA PEHLVAN KARAKA , HAYRIYE ORALLAR , HAMIT COKUN

ABSTRACT
The Effects of Lycium barbarum (Goji berry) Polysaccharides on 17--estradiol Serum Levels
of Ovariectomized Female Rats Lycium barbarum (Kurt zm) Polisakkaritlerinin Ovarektomili Dii
Sanlarn Serum 17--stradiol Seviyeleri zerine Olan Etkileri Bihter Gke BOZAT1, Fatma
PEHLVAN KARAKA1,2, Hayriye ORALLAR3, Hamit COKUN4 1Abant Izzet Baysal
University, Department of Biology, Faculty of Science and Art, Bolu, Turkey 2Abant Izzet Baysal
University, Department of Field Crops, Faculty of Agriculture and Natural Sciences, Bolu, Turkey
3Abant Izzet Baysal University, Department of Poultry Breeding, Faculty of Agriculture and Natural
Sciences, Bolu, Turkey 4Abant Izzet Baysal University, Department of Psychology, Faculty of
Science and Art, Bolu, Turkey Email: bozatgokce@gmail.com Abstract Menopause is a physiological
and endocrinological process that lead to a diminish in circulating levels of the female sex steroids
naturally or after surgery. During menopause, both diminish in 17-beta-estradiol (17- ES) and the
increment in follicle-stimulating hormone trigger several alterations in the body. Healthy womens
reproductive life span is avarage 36 years, with an age range varying between 40 and 60 years.
Although bilaterally surgical removal of ovaries has been applied for treatment in women with
endometrial and ovarion cancer, but it has been applied for ovariectomy model in rodents. The
ovariectomy has been used to clarify insufficiency of estrogen hormone and its metabolic results to
rodents. Estrogens are steroid hormones found in three form (estrone, 17- ES and estriol) in the
women body, protect and regulate life of neurons and glial cell and regulation of brain functions. Goji
berry belongs to genus Lycium of the family Solanaceae. The fruits used in herbal medicine and health
food for thousands of years in China, Southeast Asia, Europe, and North America. Goji berry can
lower blood lipid levels and promote fertility. Goji berries have carotenoids, zeaxanthin and
polysaccharides. Especially, its polysaccharides importantly alleviated neuronal injury and obstructed
lactate dehydrogenase release. Furthermore, it was also reported that Lycium barbarum
polysaccharides (LBP) could prevent cognitive and memory deficits. Similarly, estrogen has
protective roles in woman body, lock of the protective roles during the menopause causes trigger many
of diseases such as behavioral, hormonal and cognitive disorders. For these similar protective roles of
estrogen and LBP on brain functions, we investigated the effects of LBP on 17--ES serum level of
ovariectomized female rats using ELISA test. Fifteen days after ovariectomy operations, rats were
divided into four major groups: control (distile water, 3 mL/kg, oral gavage, per day), low dose of
LBP (20 mg/kg, 3 mL/kg, oral gavage, per day), high dose of LBP (200 mg/kg, 3 mL/kg, p.o., per
day), 17--ES (1 mg/kg, 3 mL/kg, oral gavage, per day) and two minor group within the each major
group: sham (pseudo ovariectomized rat) and overiectomized (ovx) rat groups. The treatments were

Corresponding Author: BHTER GKE BOZAT, bozatgokce@gmail.com

1
ABANT ZZET BAYSAL NVERSTES

977
applied for 30 consecutive days and then serum sample of all rats were collected. 17- ES analysis of
the samples were performed by ELISA test. The findings of the experiment showed that distile water
treated-sham operated group had higher serum level of 17--ES than distile water treated-ovx operated
group (p< 0.05). This means that ovariectomy model caused decreasing 17- ES serum level.
Furthermore, the high dose LBP, low dose of LBP and 17- ES applications showed a similar increase
in 17- ES serum levels of ovarictomized rats compared to control group (distile water treated) (p<
0.05). In conclusion, LBP treatments may be perform protective and regulator roles on the brain by
increasing 17- ES serum level. Keywords: Goji berry, Lycium barbarum polysaccharides,
Ovariectomy, 17--estradiol, ELISA Acknowledgement: This work was supported by grants from the
Abant Izzet Baysal University Research Foundation (Project No: 2016.10.07.956).

KEYWORDS
Goji berry, Lycium barbarum polysaccharides, Ovariectomy, 17--estradiol, ELISA

978
 Poster Session 7
Submission ID: 879

CULTIVATION OF PLEUROTUS OSTREATUS AND THE PROBLEMS

ENCOUNTERED IN TOKAT PROVINCE

1 1
BRAHIM TRKEKUL , LEVENT GKREM

ABSTRACT
Today, the production of Pleurotus ostreatus (Oyster mushroom) is increasing day by day both
in the world and in our country. The problems encountered during the production of Pleurotus
ostreatus mushroom at Gaziosmanpaa University mushroom production establishment and the
problems of small scale enterprises in Tokat province were investigated. As a result of the work, it has
been observed that there are problems in marketing of the mushrooms produced, the enterprises where
the mushroom production is made are not sufficiently modernized, and the producers do not have
sufficient equipment about mushroom growing.

KEYWORDS
Mushroom cultivation, Oyster mushroom, Pleurotus ostreatu

Corresponding Author: BRAHM TRKEKUL, ibrahim.turkekul@gop.edu.tr

1
GAZOSMANPAA NVERSTES

979
 Poster Session 7
Submission ID: 883

USE OF SOME MEDICINAL AROMATIC PLANTS IN DAIRY

PRODUCTS

2 1 1
BRA GNC , MUSA SERDAR AKIN , KAMILE BURCU BYKKILI

ABSTRACT
Turkey has an important place that geographical location, climate, plant diversity and flora
with regard in trade of medical and aromatic plants. Today, about 50% of this plants, which are used
in many areas including medicine, cosmetics, perfume, paint etc. industry, has been used in food
sector. In food industry, it is offered as consumption of spices, herbal tea and food additives in terms
of functionality. In recent years, with increasing demand for minimum treatment and natural additives
products, the studies on the active ingredients in essential oils, which obtained from medicinal
aromatic plants and their extracts, have also gained momentum. It has been found that these
components both give flavor and prolong the shelf life of the foods by the antioxidative effect and
bacteriostatic / fungustatic effect, which are prevented oxidative rancidity, and microbial spoilage.
Nowadays, studies has been increased which benefit from antimicrobial compounds naturally found in
spices instead of chemical and synthetic antimicrobial agents. In this regard when looking at the
studies done, has been seen the most use of thyme, black cumin, pepper (pulbiber, isot), cumin,
cinnamon like spices, extracts and oils that high antimicrobial and antioxidant capacities. In this
review has been searched use of thyme, black cumin, pepper, cumin and cinnamon in dairy products
and showing antimicrobial effects.

KEYWORDS
Spice, antimicrobial effect, dairy products

Corresponding Author: BRA GNC, busragoncu@harran.edu.tr

2
HARRAN NVERSTES MHENDSLK FAKLTES GIDA MHENDSL BLM

980
 Poster Session 7
Submission ID: 884

DETERMINATION OF VITAMIN A, C AND E AMOUNTS IN

PLEUROTUS OSTREATUS (OYSTER MUSHROOM) SAMPLES
COLLECTED FROM NATURAL HABITATS AND CULTURED
MUSHROOMS

1 2 1
BRAHIM TRKEKUL , AYDIN KR BENG , MER FARUK TRKEKUL

ABSTRACT
Oyster mushroom (Pleurotus ostreatus) production is on the increase both in our country and
in the World each day. Naturally grown and cultured macro fungi are consumed as food. Fungi have
been very important for human nutrition and health since ancient times. In addition to having
necessary proteins for human nutrition, fungi are also rich in vitamins and minerals, which have
important functions for health. Oyster mushroom (P. ostreatus) samples grown in Gaziosmanpaa
University mushroom establishment and naturally collected from Tokat region were examined in
terms of vitamins A, C and E content. Vitamins A, C and E in fungal samples collected from natural
environment were found as; 73.38 mg / g; 1531, 40 mg / g and 1143.22 mg / g tissue while in cultured
mushroom samples they were found as: 10.08 mg / g, 352.60 mg / g and 763.32 mg / g tissue
respectively.

KEYWORDS
HPLC, Pleurotus ostreatus, vitamins (A, C, E)

Corresponding Author: BRAHM TRKEKUL, ibrahim.turkekul@gop.edu.tr

1
GAZOSMANPAA NVERSTES
2
BNGL NVERSTES

981
 Poster Session 7
Submission ID: 886

IN VITRO MICROPROPAGATION OF HYPERICUM PRUINATUM

BOISS& BAL.

1 2 3
EYMA NL , HUSSEIN ABDULLAH AHMED AHMED , SERKAN URANBEY

ABSTRACT
Hypericum pruinatum Boiss & Bal. is a medicinal plant spread out in Blacksea region in
Turkey. Hypericum species have medicinal properties such as antidepressant, antibacterial and
antivirale and has been used traditionally for many years in the world. Secondary metobolites contents
of the species are very rich and plant tissue culture applications represent a potential source of
valuable bioactive compounds. The goal of the research was also to achieve high compact, friable calli
production and regeneration of H. pruinatum. by somatic embryogenesis or organogenesis. The seeds
of H. pruinatum were collected from Amasya-Gmhacky district and incubated in a water solution
containing 1.5 g /L GA for breaking dormancy. The seeds were surface sterilized and germinated on
MS medium containing 0.005 mg/L GA and 0.6 % agar. Leaves, axillary buds, root and
hypocotyledon explants were excised and cultured on MS medium supplemented with B5 vitamins.
The compact and suitable calli production obtained on MS callus induction medium containing 2 mg/L
2,4-D using axillary buds. Axillary buds gave the best regeneration results in all media tested. The best
adventitious shoot regeneration was also achieved on MS medium supplemented with B5 vitamins and
1mg/L BAP+0.5 mg/L NAA and 1mg/L BAP. The shoots were successfuly rooted on MS medium
medium contaning 1.5 mg/L IBA and rooted plantlets were transferred to torf and perlit (1:1) mixture
and acclimatized in greenhouse conditions with high survival ratio.

KEYWORDS
Hypericum pruinatum Boiss&Bal., in vitro, medicinal plant, axillar buds, micropropagation

Corresponding Author: EYMA NL, s.sofuoglu@alparslan.edu.tr

1
MU ALPARSLAN UNVERSTY SCENCE AND ART FACULTY DEPARTMENT OF MOLECULAR BOLOGY
AND GENETCS, MU
2
DEPARTMENT OF FELD CROPS, FACULTY OF AGRCULTURE AND NATURAL SCENCES, UAK
UNVERSTY, UAK
3
DEPARTMENT OF FELD CROPS, FACULTY OF AGRCULTURE, ANKARA UNVERSTY, ANKARA

982
 Poster Session 7
Submission ID: 888

CAULERPIN, A BISINDOLE ALKALOID, FROM CAULERPA SPP OFF

THE TURKISH COASTLINE

1 1
SONGL TURHAN , LEVENT CAVAS

ABSTRACT
Caulerpa cylindracea and Caulerpa taxifolia are the most famous members of Genus Caulerpa
because of their invasive properties. Although the number of publications on C. taxifolia is higher than
that of C. cylindracea, the latter one has invaded more areas compared to C.taxifolia. Turkey is one of
the countries where C.cylindracea has invaded. No eradication method has been proposed for these
species, therefore the biotechnological evaluation methods are of great importance. Recent studies
show medicinal importance of secondary metabolites of Caulerpa genus. One of the secondary
metabolites is bisindole based caulerpin. Anticancerogen effect has been recently attributed to
caulerpin. Therefore, the monitoring of caulerpin within invasive and non-invasive species is
important for their biotechnological evaluation. In this study, the levels of caulerpin were analysed in
the C.cylindracea and C.prolifera collected off the zmir coastlines (Turkey). Since there is no
authenticate caulerpin standard available in market, caulerpin was first isolated from C.cylindracea
and then characterized by means of chromatographic techniques. According to the results, the levels of
caulerpin in C.cylindracea are significantly higher than that of non-invasive C.prolifera. In conclusion,
the conditions related to biotechnological production of caulerpin from invasive C. cylindracea are
strongly recommended.

KEYWORDS
Caulerpa cylindracea, Caulerpa prolifera, Caulerpin, invasive species.

Corresponding Author: LEVENT CAVAS, lcavas@deu.edu.tr

1
DOKUZ EYLL NVERSTES

983
 Poster Session 7
Submission ID: 890

DENIZLI DE TRUFFLE MUSHROOMS

1 1
KADIR NL , MUSTAFA KORUCU

ABSTRACT
ABSTRACT Truffle mushroom known as black diamond in the world has come to the agenda
of Turkey widely in Denizli city. Denizli Forest Regional Directorate has determined which tuff
mushroom species are in our records. Tuber aestivum (summary truffle) is usually found widespread
in fungus flora of Denizli. The trees with truffle mushroom were controlled its productivity taking
under protection. Our staffs were informed about truffle by Truffle experts from the USA and
European Union. Sapling infected with mycorrhizae of Tuber melenosporum (winter truffle) was
planted for the purpose of the experiment. When detected that Turkey was rich in the direction of
truffle, a species action plan comprising 2014-2018 years was prepared by General Directorate.
Sapling infected with mycorrhizae of Tuber melenosporum (winter truffle) was planted for the purpose
of the experiment. Oak seedlings infected with truffle mycorrhizae were produced by Seedlings
Directorate in Regional Directorate. For this purpose, a special greenhouse was build and taken
equipment. The necessary seedlings will be provided here for erection of artificial truffle forests that
will be generated in constitution of species action plan of Forest General Directorate.

KEYWORDS
Tuber aestivum, Tuber melenosporum, mycorrhiza

Corresponding Author: KADR NL, kadirunlu@ogm.gov.tr

1
ORMAN GENEL MSRL

984
 Poster Session 7
Submission ID: 892

USE OF PROBIOTICS AND PREBIOTICS IN CHRONIC KIDNEY

DISEASE

1 1
FERAY GENER , HILAL YILDIRAN

ABSTRACT
Chronic kidney disease is defined as decrease in glomerular filtration rate that would cause
significant changes in kidney function. Uremic syndrome is a clinical manifestation of severe renal
insufficiency and is intoxication affecting many systems. In healthy individuals, uremic toxins are
cleared by the kidneys, but these toxins cause to uremic syndrome by accumulating in individuals with
renal insufficiency. In uremic syndrome, toxins such as phenols and indole are formed throughout the
gastrointestinal tract and impaired intestinal microbiota has an important role in their production.
Potential use of intestinal microbiota-regulating treatments such as probiotics has emerged as an
attractive strategy to reduce uremic toxins. Experimental and clinical data have strengthened the
hypothesis that probiotics have a therapeutic role in the protection of the gastrointestinal tract, the
progression of chronic kidney disease, and the reduction of uremic toxin formation. It is believed that
probiotics make this effect by blocking the passage of pathogens through the mucosa and reducing
intestinal permeability. Prebiotics, which are indigestible nutrients, have a positive effect because they
stimulate the activity and development of bacteria in the colon. Nutritional resources of probiotics and
prebiotics usually contain high levels of sodium, potassium, phosphorus and sugar. For this reason,
patients with renal insufficiency have difficulty in taking probiotic and prebiotics with nutritional
resources, and supplementation is an alternative. Individuals have different microbiota diversity. For
this reason, increase in strain type of supplementation suggests that it increases the positive effect.
Personal biota analysis together with evolving technology can be an important guide for
supplementation.

KEYWORDS
Chronic kidney disease, probiotic, prebiotic

Corresponding Author: FERAY GENER, gencerferay@gmail.com

1
GAZ NVERSTES, SALIK BLMLER FAKLTES

985
 Poster Session 7
Submission ID: 893

USEFUL PLANTS USED AS HERBAL TEA IN TOSYA (KASTAMONU)

DISTRICT

1 2 3
GAMZE TUTTU , GKHAN ABAY , INASI YILDIRIMLI

ABSTRACT
Introduction: The relationship between man and plants is as old as human history. Some plants
have been used as food sources and some of them in the treatment of diseases. In the course of time,
humans learned to identify plants in the nature, benefit from them and developed different forms of
usage. One of the common usage forms is to brew the tea. To prepare a drink, the plant parts (leaves,
fruits, barks, branch etc.) are brewing (pouring boiling water on the plant and waiting for 5 min-
infusion) or boiling (putting the plant in cold water for half an hour by boiling-decoction). This drink
is generally named tea. Also, tea has special names according to the prepared herbs (linden tea, sage
tea, lemon balm tea etc.). In our country, tea is often consumed as food or in order to benefit from its
medicinal properties (to increase body resistance and the treatment of diseases). The aim of this study
is to determine the plants used as herbal tea in Tosya district and to present usage purposes. Material
and Methods: The plants specimens were collected from Tosya district, Kastamonu province, in 2014-
2016 within the fieldwork of the doctoral program. The specimens were identified by using Flora of
Turkey and the East Aegean Islands'. Interviews were carried out with local people in Tosya center
and villages to determine the ethnobotanical usages of plants. Also we went to Tosya Bazaar to get
information about plant usages from sellers and villagers. In addition, a survey about edible plants
and medicinal plants was applied to 217 people. This study includes, plants used as herbal tea and
their usage purposes is presented. Results: In this study, as a result of the interviews and surveys 48
taxa were determined used as herbal tea in Tosya. One of them is food, 34 are medical and 13 are both
food and medical purposes. Most of the plants (25 taxa) used to prepare herbal tea are collected from
nature and some of them are cultivated (16 taxa). However some plants which are not growing in
Tosya (7 taxa) are taken from the bazaar. Some of the diseases commonly treated with herbal tea are:
cold, cough, flu, stomach diseases, indigestion, rheumatism, urethritis, diabetes and cancer.
Acknowledgement: We thank to ankr Karatekin University scientific research projects unit (BAP-
project no: of12035d02), for the support provided for this project.

KEYWORDS
Herbal tea, Ethnobotany, Useful plants, Tosya, Turkey

Corresponding Author: GAMZE TUTTU, gamzeertugrul@karatekin.edu.tr

1
ANKIRI KARATEKN NVERSTES, ORMAN FAKLTES, ORMAN MHENDSL BLM, 18200
ANKIRI
2
RECEP TAYYP ERDOAN NVERSTES, PEYZAJ MMARLII BLM, BTK MATERYAL VE
YETTRCL ANABLM DALI, RZE
3
HACETTEPE NVERSTES, FEN FAKLTES, BYOLOJ BLM, 06800 BEYTEPE, ANKARA

986
 Poster Session 7
Submission ID: 894

EVALUATION OF ANTIOXIDANT AND ANTICHOLINESTERASE

ACTIVITIES OF IRIS XANTHOSPURIA EXTRACTS GROWING IN
KYCEIZ REGION

1 2 1 1
MEHMET ALI ZLER , YUSUF SICAK , MEHMET ZTRK , MEHMET EMIN DURU

ABSTRACT
The effect of oxidation on human health has been widely recognized. Oxidative metabolism is
compulsory for the survival of cells. An antioxidant may be defined as a substance that significantly
delays or inhibits the oxidation of a substrate even at low concentrations when compared with
oxidizable. Antioxidants play an important role in the prevention and cure of various of chronic
diseases such as cancer, cardiovascular, atherosclerosis, stoke, diabetes, and Alzheimers diseases. The
study was aimed to determine antioxidant and anticholinesterase activities of various extracts; namely,
n-hexane, dichloromethane, ethyl acetate and n-butanol obtained from rhizome and stem of Iris
xanthospuria. The in vitro antioxidant activity were performed by four complementary assays, namely,
ABTS cation radical scavenging, -carotene-linoleic acid, CUPRAC and DPPH free radical
scavenging methods, while anticholinesterase activity performed according to Ellman method in
which acetylcholinesterase and butyrylcholinesterase were used as enzymes. The ethyl acetate extract
of rhizome and stem showed the highest antioxidant capacity and anticholinesterase activity. The
results for both activities were close to those of standards. Compounds present in this plant are good
candidates for isolation and commercial use as antioxidants and anticholinesterase agents.

KEYWORDS
Iris xanthospuria, antioxidant activity, anticholinesterase activity

Corresponding Author: YUSUF SICAK, yusufsicak@mu.edu.tr

1
DEPARTMENT OF CHEMSTRY, FACULTY OF SCENCES, MULA SITKI KOMAN UNVERSTY, 48210,
MULA, TURKEY
2
DEPARTMENT OF HERBAL AND ANMAL PRODUCTON, KYCEZ VOCATONAL SCHOOL, MULA SITKI
KOMAN UNVERSTY, 48800, MULA, TURKEY

987
 Poster Session 7
Submission ID: 895

AN ASSESSMENT OF NON-WOOD FOREST PRODUCTS IN TURKEY

1 1 1
STNER BIRBEN , SERHAT URSAVA , HASAN EMRE NAL

ABSTRACT
Forest resources produce a variety of benefits such as direct use values, indirect use values,
option values and existence or non-use values. Wood has been the most recognized economic product
form while Non-Wood Forest Products (NWFPs) have been labelled as minor forest products. The
term NWFPs encompasses all biological materials other than wood, and may include foods,
medicines, spices, essential oils, resins, gums, tannins, dyes, ornamental plants, water and wildlife.
Turkey has a spectacular geography with its natural passageway between Asia and Europe, and is at
the junction of Mediterranean, Irano-Turanian, and Euro-Siberian phytogeographic regions, besides
being under the influence of Mediterranean, continental, oceanic climates. These unique properties put
Turkey among the richest countries with regard to biodiversity that covering diverse flora and fauna
values. That is why Turkey has great potential in NWFPs. This study sought to introduced NWFPs
important in Turkey and to reveal the economic value of NWFPs in the national market and to
determine both the difficulties in managing of NWFPs and the policies, strategies and regulations that
govern, and finally, various deliberations and suggestions have been made on the axis of sustainable
development of that resources.

KEYWORDS
Non-Wood Forest Products (NWFPs), Trade, Sustainability, Turkey,

Corresponding Author: STNER BRBEN, birben@karatekin.edu.tr

1
ANKIRI KARATEKN NVERSTES ORMAN FAKLTES

988
 Poster Session 7
Submission ID: 896

A ETHNOBOTANICAL RESEARCH ON WILD FRUITS OF BNGL

1 2 2
RIDVAN POLAT , EBRU YCE BABACAN , UUR AKILCIOLU

ABSTRACT
This study carried out between May 2012 and September 2014 proposed to identify wild
frutits and the diverse ways they are used by the local populations of Bingl. During this period, 56
vascular plant specimens were collected. The plants were pressed in the field and prepared for
identification. A total of 22 wild fruit plants belonging to different families were identified in the
region. In the research area, local people were found to use wild fruits for food and for curative
purposes. These plants are used in the treatment of many diseases. By drying infusions or decoctions
of these plants, local people use them during the whole seasons of the year. Most commonly used
plants genus are Crataegus, Rosa, Rubus and Pyrus.

KEYWORDS
Etnobotany, Traditional medicine, Food plants, Wild fruits, Bingl.

Corresponding Author: RIDVAN POLAT, rpolat10@hotmail.com

1
BNGL NVERSTES
2
MUNZUR NVERSTES

989
 Poster Session 7
Submission ID: 897

CEREALS AND ANTHOCYANNS

1 1
ALA KAYOLU , MNR ANIL

ABSTRACT
Cereals and Anthocyanins ala KAYOLU, Mnir ANIL Ondokuz Mays University
Engineering Faculty Food Engineering Department, Samsun The anthocyanins compose a group of
intensely coloured pigments responsible for the orange, red, purple and blue colours of many fruits,
vegetables, flowers, leaves, roots and other storage organisms of plants. They are found in nature in
the form of polyhydroxylated and or methoxylated heterosides which derive from the flavylium ion or
2-phenylbenzopyrilium. The de-glycosylated or aglycone forms of anthocyanins are known as
anthocyanidins. Aglycon (anthocyanidin) is found united to one or various sugars, which, in turn, can
be acylated with different organic acids. The presence of these hydroxyl groups on the rings, as well as
one or several sugar molecules, make these compounds quite soluble in water, ethanol, and methanol.
Anthocyanin stability increases with the number of methoxyls in the B ring and decreases as
hydroxyls increase. Thus, among the most common anthocyaninidins, the most stable is malvidin,
followed by peonidin, petunidin, cyanidin and delphinidin. The differences between anthocyanins
relate to the number of hydroxyl groups, the nature and number of sugars attached to the molecule, the
position of this attachment, and the nature and number of aliphatic or aromatic acids attached to sugars
in the molecule. Anthocyanins occur naturally in fruits and vegetables as glycosides, having glucose,
galactose, rhamnose, xylose or arabinose attached to an aglycon nucleus. Purple corn is a special
cultivar of corn that is rich in anthocyanins and other functional phytochemicals. The health benefits
of anthocyanins in purple corn have been attributed to their high antioxidant activities and to other
mechanisms, such as the presence of components that have been shown to potentially reduce the risk
of colon cancer by inhibiting the proliferation of human colon cancer cells in vitro. The anthocyanins
of purple corn have been characterized and these include cyanidin-3-glucoside, cyanidin-3-(6-
malonylglucoside), cyanidin-3-(3, 6-dimalonylglucoside), pelargonidin-3-glucoside, peonidin-3-
glucoside and their malonated counterparts as the major anthocyanins. Although widely consumed as
white rice, there are many special cultivars of rice that contain colour pigments, such as black rice, red
rice and brown rice. Their name refer to the kernel colour (black, red or purple) which is formed by
deposits of anthocyanins in different layers of the pericarp, seed coat and aleurone. The most abundant
colored rice anthocyanins are cyanidin 3-glucoside and peonidin 3-glucoside with cyanidin 3-
glucoside levels being significantly higher than peonidin 3-glucoside. The functional properties of the
extracts from black rice have been widely studied using in vivo and in vitro models. Because of their
high antioxidant activity, they can protect endothelial cells prevent heart and cardiovascular diseases
and act as anticancer agents. Whole wheat grain is a good source of dietary fiber and antioxidants
which can promote health benefits towards several chronic diseases usually associated with oxidative
stress. Although most of the cultivated cultivars are white or red-grained, some varieties such as
purple and blue wheat grains have drawn the attention of researchers and food industry due to their
high content in anthocyanin pigments and to their antioxidant properties. Color in wheat grains is

Corresponding Author: ALA KAYOLU, cagla.kucuksozen@gmail.com

1
ONDOKUZ MAYIS NVERSTES, MHENDSLK FAKLTES, GIDA MHENDSL BLM

990
localized in the bran layers. The red color is due to the presence of major catechin-tannin and minor
anthocyanins in the diploid testa of seed coat. The purple color is due to anthocyanins and in the
diploid pericarp layer. Blue color is due to anthocyanins in the aleurone layer. Anthocyanins in purple
wheat; delphinidin 3-galaktosid, delphinidin 3-arabinosid, siyanidin 3-arabinoz, petunidin 3-
galaktosid, siyanidin 3-glukozid, peonidin 3-glukosid, malvidin 3-glukosid 3-galaktosid, pelargonidin
3-arabinosid ve peonidin 3-arabinosid, malvidin 3-glukosid, siyanidin. Siyanidin 3-glikosid was also
the most abundant anthocyanin in purple wheat; however, this was followed by siyanidin 3-galaktosid
and malvidin 3-glikosid. Blue wheat, reported the most abundant anthocyanin to be delphinidin-3-
glucoside followed by delphinidin-3-rutinoside, accounting for %37 and %32 of the total anthocyanin
content, respectively. Cyanidin-3-glucoside and peonidin-3-glucoside have also been detected in
purple wheat reported that cyanidin-3-glucoside was the principal anthocyanin in aleurone blue wheat
with pelargonidin 3-glucoside and cyanidin-3-galactacide also being present. In barley, delphinidin 3-
glucoside, delphinidin 3-rutinoside, cyanidin 3-glucoside, petunidin 3-glucoside, and cyanidin chloride
were positively identified with delphinidin 3-glucoside and cyanidin 3-glucoside being high in yellow
and purple barley, respectively.

KEYWORDS
anthocyanin, wheat, corn, rice, barley

991
 Poster Session 7
Submission ID: 898

ETHNOMEDICINAL USES OF ONOPORDUM ACANTHIUM L. IN

BINGL REGION

1 2 2
RIDVAN POLAT , UUR AKILCIOLU , EBRU YCE BABACAN

ABSTRACT
This study, conducted between 2011-2012, gathered information on the medicinal uses of
Onopordon acanthium L. in Bingl region. Onopordon acanthium L. from Asteraceae family is a
biennial plant growing in the Asia and Europe. Onopordon acanthium L. is an important medicinal
plant which has been used in folk medicine. In the research area, local people were found to use
Onopordon acanthium for curative hemorrhoids. In this study, collection, drying and usage phases of
Onopordon acanthium were examined.

KEYWORDS
Etnobotany, Traditional medicine, Onopordon acanthium, Bingl.

Corresponding Author: RIDVAN POLAT, rpolat10@hotmail.com

1
BNGL NVERSTES
2
MUNZUR NVERSTES

992
 Poster Session 7
Submission ID: 899

A REVEW OF DETARY TOTAL ANTIOXIDANT CAPACITY AND

CORONARY ARTERY DEASE

1 1 1 1
NESLIHAN ARSLAN , GAMZE AKBULUT , NILFER ACAR TEK , GLSM DEVEC

ABSTRACT
The relationship between diet and health have been an important issue. The roles of dietary
antioxidants and health also have been of particular interest. Compounds in fruits and vegetables are
bioactive and they interact with each other. These compounds are antioxidants. The Institute on
Medicine has defined a dietary antioxidant as a substance in foods that significantly decreases the
adverse effects of reactive species (oxygen and nitrogen species) on normal physiological function in
humans. Reactive oxygen species can form as a result of metabolic and physiological processes and as
oxidative reactions in the organism. The organism can prevent these reactive oxygen species with
enzymatic and non-enzymatic antioxidative mechanisms. Under some circumstances, the increase of
oxidants and the decrease of antioxidants may not be prevented and the oxidative/antioxidative
balance may shift towards the oxidative state. As a result, oxidative stress is responsible for more than
100% of the disease. Antioxidant molecules prevent oxidative reactions that occur in the organism.
The total antioxidant capacity (TAC) aims to measure the free radical-reducing capacity of all
antioxidants in the diet and takes into account synergistic effects between substances. Measuring the
total antioxidant capacity of the diet is a challenge and different alternatives have been explored
which show similar ranking of foods according to their antioxidant capacity. Concentrations of
different antioxidants in serum can be measured separately in the laboratory environment. But these
measurements are both time consuming, expensive and complicated. Since individual measurements
of different antioxidant molecules are impractical and the effects of these antioxidants are synergistic,
an antioxidant response is measured, and this is named Total Antioxidant Capacity (TAC). Dietary
intake of antioxidants has been reported to reduce the risk of many metabolic diseases. However, it is
not clear which antioxidant is more effective in reducing this risk, but there are also studies showing
the ineffectiveness or adverse effect of using a single antioxidant. Total antioxidant capacity reflects
all antioxidants in the diet and synergistic effects of these antioxidants. Dietary total antioxidant
capacity and serum antioxidant capacity were found to be inversely related to coronary artery diease
according to the data obtained from epidemiological studies. At the same time the antioxidant capacity
of serum is related with coronary artery diease. The purpose of this rewiev is to demonstrate the
relationship between dietary and serum total antioxidant capacity and coronary artery diease. In a
study of 53 coronary artery 42 healthy control groups, serum TAC levels in the patient group were
significantly lower than in the healthy control group (p<0.001). TAC levels were significantly lower in
diabetic, smokers, hyperlipidemic and obese subjects compared to those who did not include these
factors [13]. In the study with individuals with coronary angiography abnormalities who admitted to
the hospital with 42 myocardial infarction under the age of 35, the TAC level was significantly higher
in the control group. TOS level was significantly higher in the patient group. The disease showed a
positive correlation with severity and TOS. As a result, increasing the total antioxidant capacity of the

Corresponding Author: NESLHAN ARSLAN, akdeniz.neslihan91@gmail.com

1
GAZ NVERSTES SALIK BLMLER FAKLTES BESLENME VE DYETETK BLM

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diet in individuals with a high risk of coronary artery disease is an important effect in preventing
disease formation.

KEYWORDS
dietary antioxidant capacity,coronary artery disease, antioxidants

994
 Poster Session 7
Submission ID: 900

DETERMINATION OF APPROPRIATE AREAS AND DESIGN

PROPOSALS FOR HEALING GARDENS IN TOKAT

1 2 3
KBRA YAZC , HEDA BASIRE AKA , LEVENT YAZC

ABSTRACT
In recent years, research on the psychological impact of the structural environment on human
health has come to the fore in the world public, resulting in the emergence of the 'healthcare design
and quality of life' approach. Healing garden designers have positive effects on users by creating
spaces with natural and artificial elements. Created spaces ensure that people are physically and
mentally healthy. Recently, in Tokat, projects related to the importance of medicinal aromatic plants as
well as their development have been carried out. However, the design of healing gardens is a fairly
new topic today and there is no public healing garden in Tokat. The purpose of this research was to
determine the appropriate areas for the healing gardens in Tokat in the light of the available research
and to explain the design principles and benefits for visitors. In conclusion, this study was described
the role of the history of the region and plant diversity in planning in the design of healing garden.

KEYWORDS
Healing garden, Landscape design, Tokat

Corresponding Author: HEDA BASRE AKA, suhedabasire@hotmail.com

1
GAZOSMANPAA NVERSTES ZRAAT FAKLTES BAHE BTKLER BLM- TOKAT
2
BLENT ECEVT NVERSTES AYCUMA GIDA VE TARIM MESLEK YKSEKOKULU - ZONGULDAK
3
ORTA KARADENZ GET KUAI TARIMSAL ARATIRMA ENSTTS MDRL- TOKAT

995
 Poster Session 7
Submission ID: 901

DETERMINATION OF ANTIBACTERIAL ACTIVITIES OF

DIFFERENT PLANT SPECIES BELONGING TO ASTERACEAE
FAMILY

1 1 1 1
DUDU DUYGU KILI , ARIF AYAR , CEREN YAVUZ , TUBA YILDIRIM

ABSTRACT
The use of herbs as alternative medicine have increased dramatically in the last years. In
recent years, due to the harmful effects of chemical drugs are frequently observed antimutagenic,
antioxidant, antibacterial and antifungal properties of extracts which derived from plants and they have
been used in medicine. In this study, aim was investigate the antibacterial activities of some plant
species belonging to Asteraceae family which distributed to different ecological conditions in Amasya.
The antibacterial activities of Anthemis tinctoria, Matricaria chamomilla and Achillea biebersteinii
plant extracts which belonging to Asteraceae family from Amasya region were determined by disc
diffusion and microdilution method. The methanolic extracts of these plants were prepared with
Soxhlet extractor. The antibacterial activities of plant extracts were tested against standard strains of
Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 35218, Klebsiella pneumoniae ATCC
70600, Pseudomonas aeruginosa ATCC 27853 and Salmonella enteritidis ATCC 13076. As a result of
this study, it was observed that plant extracts had antibacterial effects when they compared with
control group antibiotics According to the results of disk diffusion method, the highest antibacterial
effect was identified Anthemis tinctoria, Matricaria chamomilla and Achillea biebersteinii
respectively. The microdilution method was studied concentration range from 6.25 - to 50 mg/ml. The
minimum inhibition concentration (MIC) of the most effective Anthemis tinctoria plant extract were
12.5 mg/ml for Klebsiella pneumoniae and Pseudomonas aeruginosa; 25 mg/ml for Staphylococcus
aureus and Escherichia coli; 25 mg/ml for Salmonella enteritidis. The MICs of the most effective
Matricaria chamomilla plant extract were 25 mg/ml for Klebsiella pneumoniae, Escherichia coli,
Salmonella enteritidis and Pseudomonas aeruginosa; 50 mg/ml for Staphylococcus aureus. The MICs
of the most effective Achillea biebersteinii plant extract were 25 mg/ml for Klebsiella pneumoniae;
50 mg/ml for Escherichia coli and Pseudomonas aeruginosa; 12.5 mg/ml for Staphylococcus aureus;
50 mg/ml for Salmonella enteritidis. As a result, We determined Anthemis tinctoria, Matricaria
chamomilla and Achillea biebersteinii plant extracts which are belonging to Asteraceae family have
properties of bacterial inhibition. In this context, it can be considered that these plant species are used
as an alternative treatments and can lead to later studies.

KEYWORDS
Asteraceae, Disc diffusion, Microdilution

Corresponding Author: DUDU DUYGU KILI, drduygukilic@gmail.com

1
AMASYA NVERSTES

996
 Poster Session 7
Submission ID: 902

TYROSINASE INHIBITORY ACTIVITIES OF IRIS XANTHOSPURIA

EXTRACTS

1 2 1 1
MEHMET ALI ZLER , YUSUF SICAK , MEHMET ZTRK , MEHMET EMIN DURU

ABSTRACT
Tyrosinase inhibitors inhibit the production of melanin in the derm. Up to date, many natural
compounds have been screened for their tyrosinase inhibitory potential, and they were compared to
those of synthetic tyrosinase inhibitors. The tyrosinase inhibitors such as arbutin, kojic acid, and
hydroquinones are used as whitening and anti-hyperpigmentation agents. In this context, there is a
need for new tyrosinase inhibitors without the side effects. The study was aimed to determine
tyrosinase inhibition activities of various extracts obtained using n-hexane, dichloromethane, ethyl
acetate and n-butanol solvents from rhizome and stem of Iris xanthospuria. In this study, it can be
concluded that the ethyl acetate extracts of I. xanthospuria rhizome can be a potential candidate for the
inhibition of tyrosinase enzyme.

KEYWORDS
Iris xanthospuria, Tyrosinase inhibition activity

Corresponding Author: YUSUF SICAK, yusufsicak@mu.edu.tr

1
DEPARTMENT OF CHEMSTRY, FACULTY OF SCENCES, MULA SITKI KOMAN UNVERSTY, 48210,
MULA, TURKEY
2
DEPARTMENT OF HERBAL AND ANMAL PRODUCTON, KYCEZ VOCATONAL SCHOOL, MULA SITKI
KOMAN UNVERSTY, 48800, MULA, TURKEY

997
 Poster Session 7
Submission ID: 903

SPIRULINA AND ITS HEALTH EFFECTS

1 1 1 1
NESLIHAN ARSLAN , GAMZE AKBULUT , NILFER ACAR TEK , GLSM DEVEC

ABSTRACT
The dried state of Arthrospira platensis, an oxygenic photosynthetic bacteria found in sea
water, is called Spirulina. The name Spirulina comes from the spiral filaments of Arthrospira platensis.
Spirulina naturally grows in alkaline lakes and seas and is commercially produced under controlled
conditions in the greenhouse. Because it has not cellulose and cell walls, it is readily digested. In this
review, spirulina's effects on health such as antioxidant, antidiabetic, antihypertensive,
antiinflammatory are discussed. THE CONTENT OF SPIRULINA 60-70% of the dry weight is
composed of protein. It contains all essential amino acids ; gamma-linolenic acid, alpha-linolenic acid,
linoleic acid, EPA, DHA, stearidonic acid and arachidonic acid. It contains vitamin B1, B2, B3, B6,
B9, C,A and E and minerals such as calcium, potassium, chromium, copper, manganese, iron,
phosphorus, magnesium, and selenium. EFFECTS ON HEALTH ANTIOXIDANT EFFECT The
antioxidant effect of spirulina has been demonstrated by in vitro and in vivo studies. This antioxidant
effect is known to be caused by phytocyanins, B-carotene and other vitamins and minerals contained
in the content. In a study conducted in hypercholesterolemic rats, 1-5 g / kg spirulina is administered
for 8 weeks. Ultimately, it has been shown to increase glutathione, glutathione peroxidase and
glutathione reductase activity in the liver. The DNA degradation in lymphocytes is significantly
reduced. ANTIDIABETIC EFFECT In a study of diabetics, SP administration reduced plasma fasting
glucose level significantly. Fluid uptake was significantly lower in the SP group than in the 2 diabetic
groups. The possible mechanism of SP is suggested to stimulate pancreatic beta cells, either to
increase insulin release or to help transport blood glucose to peripheral tissues. NUTRITION STATUS
A study in which 87 malnourished children aged 0-5 years were given 3 grams of spirulina for 12
weeks showed significantly better height and body weight when compared to the vitamin and mineral
treated group. It is also more effective than vitamin-mineral treated group at ferritin and iron levels.
TOXICITY There is no information yet on the toxicity of spirulina. However, it is said that potentially
toxic materials such as heavy metals can be found in the water. It has also been reported that pesticides
may contain cyanobacterial toxins. That is why Spirulina should be acquired from trusted sources.
Spirulina usually works at 1-10 g/day doses. Doses recommended for adults are 3-10 g/day. The
Dietary Supplements Information Expert Committee (DSI-EC) has shown that spirulina does not
constitute a class of health hazard. However, it is still unclear whether it interacts with other
medicines. Headache, stomachache, muscle pain and concentration problems have been reported in
some cases. It is also not recommended for use in patients with phenylketonuria, autoimmune diseases
and ALS . Spirulina is generally recognized as safe(GRAS list). In 2012, it was found safe to use both
spirulina and extract as a coloring agent. The dose with no observable side effects (NOAEL) was
determined to be 10.00 mg/ kg. The safe dose was 4,12 mg/kg according to the information obtained
from human studies. CONCLUSIONS AND RECOMMENDATIONS Human studies done up to
daylight are rather limited. More case-control studies are needed. Given its composition, spirulina is

Corresponding Author: NESLHAN ARSLAN, akdeniz.neslihan91@gmail.com

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998
said to be a rich source of protein, vitamins, minerals and phytochemicals. The use of this source in
poor and malnutrition-fighting countries is being debated. In order to be cheap and accessible, studies
on the use of spirulina for this purpose should be undertaken.

KEYWORDS
Spirulina,Arthrospira platensis, Nutrition

999
 Poster Session 7
Submission ID: 904

ANTIBACTERIAL EFFECTS OF METHANOL EXTRACTS OF SOME

PLANT SPECIES BELONGING TO LAMIACEAE FAMILY

1 1 1 1
DUDU DUYGU KILI , ARIF AYAR , CEREN YAVUZ , TUBA YILDIRIM

ABSTRACT
The effects of medicinal and aromatic plants have been known since ancient times and these
plants are widely used in the treatment of diseases. Especially the species belonging to Lamiaceae
family are used as antibacterial agent among medical plants. The aim of the present study was to
determinated the antibacterial effects of the methanolic extracts of some plant species belonging
Lamiaceae family which distributed to different ecological conditions in Amasya. The antibacterial
effects of Stachys annua, Scutellaria salviifolia and Nepata nuda plant extracts was determined by disc
diffusion and microdilution methods. The methanolic extracts of these plants were prepared with
Soxhlet extractor. The antibacterial effects of plant extracts were tested against standard strains of
Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 35218, Klebsiella pneumoniae ATCC
70600, Pseudomonas aeruginosa ATCC 27853 and Salmonella enteritidis ATCC 13076. In this study,
we observed that plant extracts had antibacterial effect when they compared with control group
antibiotics. According to the results of disk diffusion method, the highest antibacterial effect was
identified Scutellaria salviifolia, Stachys annua and Nepata nuda respectively. The microdilution
method was studied concentration range from 6.25 - to 50 mg/ml. The minimum inhibition
concentrations (MIC) of the most effective Scutellaria salviifolia plant extract were 12.5 mg/ml for
Staphylococcus aureus, Salmonella enteritidis and Escherichia coli; 25 mg/ml for Klebsiella
pneumoniae and Pseudomonas aeruginosa. The MICs of Stachys annua plant extract were 12.5 mg/ml
for Pseudomonas aeruginosa, Salmonella enteritidis and Escherichia coli; 25 mg/ml for
Staphylococcus aureus; 50 mg/ml for Klebsiella pneumoniae. MICs of Nepata nuda plant extract were
12.5 mg/ml Klebsiella pneumonia; 25 mg/ml for Staphylococcus aureus; 50 mg/ml for Pseudomonas
aeruginosa, Salmonella enteritidis and Escherichia coli. As a result, Our study it was determined that
Scutellaria salviifolia, Stachys annua and Nepata nuda species belonging to the Lamiaceae family have
antibacterial activities in vitro conditions. After investigating toxicological and pharmacological
properties, we think that it may be the subject of use in medicine, food, cosmetics and other industrial
fields.

KEYWORDS
Lamiaceae, Disc diffusion, Microdilution

Corresponding Author: DUDU DUYGU KILI, drduygukilic@gmail.com

1
AMASYA NVERSTES

1000
 Poster Session 7
Submission ID: 906

ANTIOXIDANT PROPERTIES OF SOME NON-WOOD FOREST

PRODUCTS AT DIFFERENT TEMPERATURES

1 2 3 1
CEYHUN KILI , ZEHRA CAN , AYENUR YILMAZ , HLYA TURNA

ABSTRACT
Some non-wood forest products are brewed and consumed as tea. Among the reasons for the
consumption of herbal tea, digestive problems are located in the first row. Antioxidants help to human
body for arranging digestive and immune system. Herbal tea is brewed in various ways such as boiling
at different durations or waiting in hot water at different temperatures etc. Type of brewing can affect
to bioactive properties of herbal tea. In this study, it was investigated the bioactive properties (total
phenolic content, total flavonoid content, condensed tannin content and antioxidant properties) of
some herbals brewed (Green tea / Camellia sinensis, senna / Zea mays ssp., corn silk / Cassia ssp.,
rosemary / Rosmarinus officinalis) at different temperature. These herbs were brewed for 10 minutes
at 60oC, 80 oC and 100 oC temperatures. After cooling, total phenolic content, total flavonoid content,
condensed tannin content and antioxidant properties of these herbs were determined. Consistently; the
highest results were found in the tea brewed at 100oC The highest total flavonoid (0.305 0.005 mg
QE/g) and ferric reducing ability (670.150 2.121 mol FeSO47H2O/g) was in Rosmarinus
officinalis. . The highest condensed tannin (9.443 0.524 mg CE/g) and the highest total phenolic
content (4.872 0.005 mg GAE/g) was in Camellia sinensis and Cassia ssp., respectively

KEYWORDS
Antioxidant, corn silk, green tea, rosemary, senna

Corresponding Author: CEYHUN KILI, ceyhunkilic@gmail.com

1
DOU KARADENZ ORMANCILIK ARATIRMA ENSTT MDRL
2
GRESUN NVERSTES
3
KARADENZ TEKNK NVERSTES

1001
 Poster Session 7
Submission ID: 908

UTILIZATION OF JERUSALEM ARTICHOKE FLOUR IN THE

MANUFACTURING OF TURKISH DRY FERMENTED SUCUK

1 1 1 1
AYEGL GNDEM , DUDU GMEN , DILEK KAZAN , CEM OKAN ZER

D
ABSTRACT
Jerusalem artichoke is a medicinal plant with various effects such as antimicrobial, antifungal

TE
and anticancer activities and it is often used as folk medicine for the treatment of some problems.
Additionally, it contains high amount of phenolic compound, antioxidants and dietary fiber. It can be
concluded that artichoke may be used as a functional food additive in food industry because of these
useful properties. The objective of this study was to investigate the effect of replacing animal fat with
Jerusalem artichoke flour on the quality characteristics of sucuk (Turkish dry fermented sausage).
EN
Experimental sucuks were manufactured with replacing animal fat with 0, 5, 10, 15 and 20% artichoke
flour. Chemical composition, color and texture properties and microbial properties of sucuks were
determined during manufacture and storage period. The results indicated that addition of artichoke
flour decreased TBARS values compared to control group (p<0.05). The use of Jerusalem artichoke
ES
flour affected lactic acid bacteria counts and fermentation process in positive manner (p<0.05).
Moreover, pH, ash and hardness values were influenced by use of Jerusalem artichoke flour (p<0.05).
Addition of artichoke flour enhanced pH decrease in sucuk during the fermentation and storage period
(p<0.05). Increasing levels of artichoke flour in sucuk formulation caused a decrease in hardness
values of sucuk (p<0.05). The results indicated that the use of artichoke flour in sucuk manufacture
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had no negative effects on quality parameters of sucuk and therefore it can be concluded that
utilization of artichoke flour in sucuk manufacture may has positive nutritional effects and may
contribute to improve shelf life of sucuk.
T

KEYWORDS
Sucuk, Jerusalem artichoke, Fermentation Texture, TBARS
O
N

Corresponding Author: AYEGL GNDEM, 13260410038@nevsehir.edu.tr

1
NEVEHR HACI BEKTA VEL UNVERSTY

1002
 Poster Session 7
Submission ID: 909

YOUTH ATTITUDES AND BEHAVIORS OF MEDICINAL PLANT OR

MEDICINAL PLANT BASED DRUG USE

1 2
ZKAN GRGL , ASLI AKILLI

ABSTRACT
Herbal treatment and alternative medicine methods are widely used in old and strong eastern
cultures like China and India and also Middle East countries like Syria, Iran, and Iraq. In the last 20
years, interest in herbal medicine has been dramatically increased in our country and Europe. When
compulsory migrations from the Middle East are expected to affect western cultures; it is envisaged
that herbal cure will become prevalent and the importance of the herbal cure will increase. This work
was supported by the Ahi Evran University Scientific Research Projects Coordination Unit. Project
Number: TIP.E2.17.006. In this study, it was aimed to determine the attitudes and behaviors of the
young population in the age of university in Turkey regarding the use of medicinal plants or medicinal
plant based drugs. For this purpose, a survey study was conducted on 400 university students.
Participation in the survey was done on a voluntary with randomly selected students. The descriptive
statistics as well as the chi-square test were used in the study. The average age of the students who
participated in the survey was calculated as 20.43 0.098. In this study conducted with a young group,
it was determined that 32.5% of the youth use medicinal plants or medicinal plant based drugs and
67.5% do not use them. Gender was statistically significant in the use of these products (p<0.01) and
also it was found that the place of birth in the rural or urban was not effective on the medicinal plant or
medicinal plant based drug use (p> 0.05). Mothers and grandparents were found to be most effective
in the use of these products in their families (p <0.01). In the study of participation from seven
geographical regions of Turkey, it was determined that there was no significant relationship between
the medicinal plant or medicinal plant-based drug use and the regions where the students' families
lived (p>0.05). However, the distribution of the medicinal plant or medicinal plant based drugs used
according to regions was statistically significant (p<0.01).

KEYWORDS
Alternative medicine, complementary medicine, herbal medicine, herbal treatment, medicinal
plant

Corresponding Author: ZKAN GRGL, ozkangorgulu@gmail.com

1
AH EVRAN NVERSTES TIP FAKLTES
2
AH EVRAN NVERSTES ZRAAT FAKLTES

1003
 Poster Session 7
Submission ID: 910

DETERMINATION OF ANTIOXIDANT AND ANTIMICROBIAL

ACTIVITIES OF SOME TAXA OF THE GENUS TANACETUM L.

1 1 2 2
YUSUF ALAN , AHMET SAVCI , SIRA TOPDEMR , MURAT KURAT

D
ABSTRACT
Tanacetum L., the third largest genus in the family Asteraceae (Compositae), which has 160

TE
taxa worldwide. Usually, the taxa of Tanacetum have spread to Europe, Asia, North Africa and North
America. In our country, 46 taxa are present. In our work, plant samples were collected in the province
of Bitlis. Extracts of Tanacetum taxa (Tanacetum aureum (lam.) Greuter var. oligocephalum (DC.)
Kandemir (Eki pireotu), Tanacetum parthenium (L.) Sch. Bip. / beyaz papatya, Tanacetum
balsamitoides L. (Marsuvanotu), Tanacetum zahlbruckneri (Nab) Grierson (zge pireotu)) were
EN
obtained from the plant samples by clevenger hydrodistillation. Antimicrobial and antioxidant
activities of extracts of Tanacetum taxa were investigated. The antimicrobial activities of the T.
aureum var. oligocephalum, T. parthenium, T. Balsamitoides and T. zahlbruckneri taxon extracts were
determined by the hollow agar method. Bacillus subtilis ATCC 6633, Staphylococcus aureus ATCC
ES
25923, Bacillus megaterium DSM 32, Enterobacter aerogenes ATCC 13048, Escherichia coli ATCC
11229, Pseudomonas aeroginosa ATCC 9027, Klebsiella pneumonia ATCC 13883, Candida albicans
ATCC 10231, Yarrowia lipolytica and Saccharomyces cerevisiae was used as test microorganisms.
Eritromisin (E-15), Ampisillin (AM- 10), Amikasin (AK-30), Rifampisin (RD-5) and Fluconazole (25
g) antibiotics were used for positive control. According to the results obtained, T. aureum var.
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oligocephalum extract showed the highest antimicrobial activity against Y. lipolytica (26 mm) and T.
zahlbruckneri extract the lowest activity against B. subtilis (11 mm). We have found that plant extracts
generally have better antimicrobial activity when compared with antibiotics that we use. Antioxidant
activities of plant extracts were examined using different in vitro methodologies such as total
antioxidant activity by ferric thiocyanate, total reducing power by potassium ferricyanide reduction
method, reduction capacity of cupric ions (Cu2+) by the Kuprak method, 1,1-diphenyl-2-picryl-
T

hydrazyl (DPPH) free radical scavenging, 2,2-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)

(ABTS) radical scavenging activity. Compared with the standard antioxidants BHA, BHT and -
O

tocopherol, reduction capacities for samples are as follows T. balsamitoides BHA > T. aureum var.
oligocephalum > BHT > -tokoferol > T. parthenium T. zahlbruckneri. According to the results of
the study,% inhibition was 70.77% for BHT, 66.35% for BHT, 62.11% for tocopherol, 61.86% for
N

extracts. When we look at the results of ABTS+ radical scavenging activity, we can say that all of
them are close to each other and show very good activity. ABTS radical activities of plants and
standards are listed as follows: BHT (%96,16) -tocopherol (%96,15) BHA (%96,04) T. aureum
var. oligocephalum (%95,99) T. balsamitoides (%95,97) T. parthenium (%95,95) T.
zahlbruckneri (%95,92).

Corresponding Author: SIRA TOPDEMR, srctpdmr@gmail.com

1
MU ALPARSLAN NVERSTES
2
BTLS EREN NVERSTES

1004
KEYWORDS
Tanacetum (Asteraceae), Exract, Antimicrobial and Antioxidant Activity

1005
 Poster Session 7
Submission ID: 911

HYPOGEOUS FUNDAL SPECES GROWN IN OSMANIYE

1 2 3 4
FUAT BOZOK , HATIRA TAKIN , HASAN HSEYIN DOAN , ABAN GNER

ABSTRACT
In the present study, we report seven truffle taxa from Amanos Mountains and Karatepe-
Aslanta regions (Osmaniye) for Turkey. These species are as follows: Tuber aestivum, T. brumale, T.
borchii, T. nitidum, T. rufum, Terfezia olbiensis and Hysterangium clathroides. Of these species, we
also report new localities for T. olbiensis and H. clathroides within Turkey.

KEYWORDS
Tuber, Truffle, Osmaniye, Turkey

Corresponding Author: ABAN GNER, sabanguneri@ogm.gov.tr

1
DEPARTMENT OF BOLOGY, FACULTY OF SCENCE AND ARTS, OSMANYE KORKUT ATA UNVERSTY
2
DEPARTMENT OF HORTCULTURE, FACULTY OF AGRCULTURE, UKUROVA UNVERSTY
3
DEPARTMENT OF BOLOGY, FACULTY OF SCENCE, SELUK UNVERSTY
4
ADANA REGONAL DRECTORATE OF FORESTRY, NON-WOOD PRODUCTS AND SERVCES UNT

1006
 Poster Session 7
Submission ID: 912

INVESTIGATION OF ANTIMICROBIAL ACTIVITIES OF EXTRACTS

OBTAINED FROM NEPETA TRANSCAUCASICA GROSSH. VE
SCUTELLARIA ALBIDA L. SUBSP. CONDENSATA (RECH.F.)
J.R.EDM. TAXA

1 1 2 2
YUSUF ALAN , AHMET SAVCI , SIRA TOPDEMR , MURAT KURAT

D
TE
ABSTRACT
Lamiaceae family in Turkey is represented by 46 genera, 577 species and 755 taxa in total. In
our work, plant material was collected in Bitlis province. The collected plant samples of Nepeta
transcaucasica (Kaf pisikotu) and Scutellaria albida L. subsp. condensata (Krk kaside) were dried and

EN
extracted with the soxhalat method. The antimicrobial activities of the extracts obtained were
investigated. The antimicrobial activities of the Nepeta transcaucasica ve Scutellaria albida L. subsp.
condensata taxon extracts were determined by the hollow agar method. Bacillus subtilis ATCC 6633,
Staphylococcus aureus ATCC 25923, Bacillus megaterium DSM 32, Enterobacter aerogenes ATCC
13048, Escherichia coli ATCC 11229, Pseudomonas aeroginosa ATCC 9027, Klebsiella pneumonia
ES
ATCC 13883, Candida albicans ATCC 10231, Yarrowia lipolytica and Saccharomyces cerevisiae was
used as test microorganisms. Eritromisin (E-15), Ampisillin (AM- 10), Amikasin (AK-30), Rifampisin
(RD-5) and Fluconazole (25 g) antibiotics were used for positive control. According to the results
obtained, Nepeta transcaucasica extract showed the highest antimicrobial activity against C. albicans
(27 mm) and extracts the lowest activity against E.coli ve P. aeroginosaa (12 mm). When we compare
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plant extracts with the antibiotics we use, it has been found that they generally exhibit similar
antimicrobial activity.

KEYWORDS
T

Nepeta transcaucasica, Scutellaria albida L. subsp. condensata , Extract, Antimicrobial

O

Activity
N

Corresponding Author: SIRA TOPDEMR, srctpdmr@gmail.com

1
MU ALPARSLAN NVERSTES
2
BTLS EREN NVERSTES

1007
 Poster Session 7
Submission ID: 915

ASTAXANTHIN, OXIDATIVE STRESS AND SENESCENCE

1 1 1 1
GLSM DEVEC , NLFER ACAR TEK , GAMZE AKBULUT , NESLHAN ARSLAN

ABSTRACT
A progression process becomes reality by each turn of Earth around the sun. In this period,
numerous systems such as hormones, proteins, immunity are affected by results of changes in gene
expression of human. In addition to gene expression, changeable factors such as type of nutrition,
environment, stresve life style end up with people s appearanceduringthis all term.Senescence ensues
from these differentiations and cumulations increasing based on it. Stochastic theories that are some of
assumptions explained by approximately over 300 theories, suggesting which senescence occurs with
accumulation of toxic products, include hypotheses of free radical, wear and tear, destructive DNA
damage, mitochondrial and lacking of cellular adaptation mechanism. Besides of degenerative
diseases, process of senescence also accelerates due to heaps and increments of free radicals in cells
and tissues and alterations in oxidation pathway depending on some metals. Natural antioxidants that
are present as ascorbic acid, caratenoids, phenolic compounds, phospholipids,sterols, reaction products
of maillard and protein-releated components in foods minimize this oxidative damage of biomolecules
and hinders oxidative cycle. Although astaxanthin, being a carotenoid source, resembles its genus,
having both hydroxy- and oxi-parts makes it different from other carotenoids. Including both bathces,
double bonds in its structure and property of polar-nonpolar-polar enable it to be more antioxidant
activity. Astaxanthin, doses of it is 2 mg/kg/day, 6 mg/day or 0.02 % of daily diet, possesses favorable
effects on reactive oxygen species by affecting activity of antioxidative enzymes such as superoxide
dismutase, catalase and glutathione peroxidase, levels of oxidative stress products such as nitric oxide,
acrolein and 8-hydroxydeoxyguanosine (8-OHdG), pathways of heme oxygenase-1, nuclear factor
like-2 (Nrf2) and phosphatidylinositol-3 kinase/protein kinase-B (PI3K/Akt), and also decreases these
cases increasing with senescence. It is mentioned that effects of different amounts of astaxanthin on
oxidative stress and aging process in this review. After all, it has been stated that effects of antioxidant
and inflammation prevention would influence positively senescence mechanisms and impressions of
aging. Astaxanthin is important with regards to awareness raising in this topic.

KEYWORDS
Astaxanthin, oxidative stress, antioxidative enzymes, senescence

Corresponding Author: GLSM DEVEC, devecigulsum@hotmail.com

1
GAZ NVERSTES SALIK BLMLER FAKLTES

1008
 Poster Session 7
Submission ID: 920

POTENTIAL EFFECTS OF ASTAXANTHIN IN THE PREVENTION OF

DISEASES

1 1 1 1
GLSM DEVEC , NLFER ACAR TEK , GAMZE AKBULUT , NESLHAN ARSLAN

ABSTRACT
Astaxanthin, belonging to xanthophylls which are oxidized derivates of carotenoids, differs
from other xanthophylls due to having both hydroxy- and oxy-groups. Hydroxyl groups in both
terminal ends gives astaxanthin polar property and middle section also does nonpolar property. While
there have been various isomers in parallel with configurations of these hydroxy groups, esterification
of astaxanthin with diverse fatty acids and their degree influence its effectiveness and half of life. Not
being activity of vitamin A during metabolized in liver, ability of passing blood-brain barrier, joining
in grey matter of brain are other specific features of astaxhantin. In addition these, taking part in
membrane structure, activities of antioxidative, anti-inflammatory and antiapoptotic make it protective
effectin diverse chronic diseases.Treatments with different quantity of astaxanthin, 25 mg/kg/day, 50
mg/kg/day, 75 mg/kg/day, 100 mg/kg/day or 720 mg/kg/day, result ina reducing in cytokine and
bacterial burden in gastric inflammation, amelioration of GLUT4 and interlinkage of insulin-substrate,
decreasing postprandial blood sugar, enhancing insulin secretion by -cells. Besides these findings,
inhibiting growth of cancer cells, enhancing cerebral edema and blood-brain barrier, attenuatingretinal
ischemic damage, increasing liveliness of sperm and ameliorating glomerular function and social
interaction in autism are also suggested. As a result, astaxanthin may be a promising in usage of it as
adjuvant to prevention and treatments of renal, cardiovascular, neurologic and diabetes mellitus
diseases. Accordingly, it has been reported to be needed more studies.

KEYWORDS
Astaxanthin, chronic diseases, antioxidative, anti-inflammatory, antiapoptotic.

Corresponding Author: GLSM DEVEC, devecigulsum@hotmail.com

1
GAZ NVERSTES SALIK BLMLER FAKLTES

1009
 Poster Session 7
Submission ID: 923

MIYTHS RELATED TO MEDICAL PLANT OLIVE IN THE AEGEAN

REGION

1 2
NEE ERDIN , ZLEM YILMAZ

ABSTRACT
In recent years there have been many studies on the effects of medicinal and aromatic plants
on human health. Turkey has an important place in the world with its geographical features and unique
culture in terms of medical plants. The richness of the Aegean region has also been noticed in ancient
times. There are many legends and mythos, which are quoted daily before the history, and various
plants are mentioned. Mythos have played an important role for the daily use of these plants for their
medical use. The history of medical plants is as old as human history. Plants are considered to be the
most precious gift of the gods to mankind. Hippocrates is known as the father of medicine and has
produced about 400 medicinal plant classifications in his books. The great Turkish Islamic scientist
Ibni Sina mentioned various plants used as medicines in his book "The Law" written in the 11th
century. In later years, Ibni Sina's books have been used as resources for many years in medical
education in Europe. In this study, the use of olives (Olea Europaea) for medical purposes throughout
the history and related myths are mentioned. Our findings are the result of the literature search.

KEYWORDS
Medicinal plant, myth, olive, olea europaea

Corresponding Author: ZLEM YILMAZ, oaylmz@gmail.com

1
AKUPUNKTUR-FTOTERAP ZEL SALIK HZMETLER, SELUK, ZMR
2
EGE NVERSTES TIP FAKLTES, FZYOLOJ ANABLM DALI, ZMR

1010
 Poster Session 7
Submission ID: 927

WATER SOLUBLE FLUORESCENT CALIX[4]ARENE AS

NARINGENIN CARRIER: ENHANCED SOLUBILITY,
CYTOTOXICITY AND CANCER CELL IMAGING

1 2 3 4
MEHMET OGUZ , ASIF ALI BHATTI , SERDAR KARAKURT , MUSTAFA YILMAZ

ABSTRACT
Flavonoids are micronutrients that are widely identified in foods of plant origin and have been
recognized potent antioxidants, possessing bioactive potential to reduce cancer risk, prevent
cardiovascular disease, and neurodegenerative disorders. Among flavanone, naringenin is regarded as
a phytoestrogen with weak estrogenic and antiestrogenic activities that inhibits proliferation of colon
cancer cells and melanoma cells. However, poor water solubility of this flavanone make it less
bioactive. To tackle problem of solubility of this anticancer drug, different methods are being used in
pharmaceutics. Moreover, in recent years, supramolecular chemistry has gained large attention in drug
delivery system and enhancemnt of solubility of the water insoluble drugs. In this connection, different
macromolecules have been used as carrier. In this study we have synthesized water soluble calixarene
containg fluorescent moity at lower rim and formed the inclusion complex with naringenin. Different
studies such as jobs plot, phase solublity and binding constant were determined. Cancer cell imaging
were carried out to observe the movement of drug in cancer cells and IC50 values were determined.

KEYWORDS
p-Sulphonatocalix[4]arene, Dansyl, Fluorescence, Flavonoid, Naringenin, Cell maging

Corresponding Author: MEHMET OGUZ, m.oguz2011@gmail.com

1
DEPARTMENT OF ADVANCED MATERAL AND NANOTECHNOLOGY, SELCUK UNVERSTY, KONYA
2
DEPARTMENT OF CHEMSTRY, SELCUK UNVERSTY, KONYA /NATONAL CENTRE OF EXCELLENCE N
ANALYTCAL CHEMSTRY, UNVERSTY OF SNDH, JAMSHORO, PAKSTAN
3
DEPARTMENT OF BOCHEMSTRY, SELCUK UNVERSTY, KONYA
4
DEPARTMENT OF CHEMSTRY, SELCUK UNVERSTY, KONYA

1011
 Poster Session 7
Submission ID: 928

MYTHOS CONCERNING DAPHNE AND ITS MEDICINAL USAGE

1 2
NEE ERDIN , ZLEM YILMAZ

ABSTRACT
Healing with medicinal plants is as old as mankind itself. The connection between human and
his search for drugs in nature dates from the far past, of which there is ample evidence from various
sources: written documents and legends. In recent years, with the growing interest of traditional
medicine, many studies have been carried out on the effects of on the effects of plants on health. The
ancient legends and myths occupies an important place for understanding the properties of medicinal
plants. This information is transmitted orally and in writing illuminate still holds today. Turkey has a
special precaution in terms of medical plants in terms of both its geographical features and the myths.
The daphne tree is one of the important medicinal plants subject to the myths in Anatolia. Laurel
leaves be obtained from the daphne tree . Turkey, the most important laurel leaves exporter country in
the world, meets about 90% of the world bay requirement. In this study, it is mentioned about mythos
and medicinal use areas related to Daphne / Laurel leaves (Laurus nobilis) plant.

KEYWORDS
Medicinal plant, myth, daphne, laurel leaves

Corresponding Author: ZLEM YILMAZ, oaylmz@gmail.com

1
AKUPUNKTUR-FTOTERAP ZEL SALIK HZMETLER, SELUK, ZMR
2
EGE NVERSTES TIP FAKLTES, FZYOLOJ ANABLM DALI, ZMR

1012
 Poster Session 7
Submission ID: 931

THE PHENOLIC PROFILE OF NEPETA CONGESTA VAR.

CONGESTA AND NEPETA CELIOTROPIFOLIA VAR.
CELIOTROPIFOLIA

1 1 2 3
ABDULSELAM ERTA , MEHMET VEYSI ALAYAN , MUSTAFA ABDULLAH YILMAZ , NESRIN HAIMI , FIRAT
4 1 4 5
AYDIN , SERKAN YIITKAN , SMAIL YENER , UFUK KOLAK

ABSTRACT
The genus Nepeta is a member of Lamiaceae family and has a worldwide distribution with
over 250 species which widely grow in Europe, Asia, North America, North Africa and in the
Mediterranean region . In Turkey, Nepeta species are represented by 41 taxa (18 of them are endemic)
and mostly distributed in East Anatolia and Taurus Mountains . Some Nepeta species have been
traditionally used as diuretic, diaphoretic, antitussive, antispasmodic, antiasthmatic, febrifuge,
sedative, spice and herbal tea . In this study, the chemical profile of ethanol extracts of Nepeta
congesta var. congesta and Nepeta celiotropifolia var. celiotropifolia were determined using LC-
MS/MS. A comprehensive LC-MS/MS method validation was developed for the qualitative and
quantitative analysis of 37 phytochemicals including 15 phenolic acids, 17 flavonoids, 3 nonphenolic
organic acids, 1 phenolic aldehyde and 1 benzopyrane. The powdered plant materials (stems, leaves,
flowers, roots and mixed parts) were extracted three times with ethanol (50 mL each) at room
temperature for 24 h. Afterwards, the extracts obtained were combined, filtered and evaporated under
low pressure. Dry filtrates were reconstituted in ethanol at a concentration of 250 mg L-1 and filtered
through the 0.2 m PTFE filter prior to LC-MS/MS analysis. These two species were found to be rich
in rosmarinic acid. Especially, their flowers extracts possessed very high amount of rosmarinic acid
and cosmosiin. Acknowledgements: The research was funded by grant: BYP-2016-20585 from
Istanbul University

KEYWORDS
Nepeta, Phenolic, LC-MS/MS

Corresponding Author: MEHMET VEYS ALAYAN, mehmetveysicaglayan@hotmail.com

1
DEPARTMENT OF PHARMACOGNOSY, FACULTY OF PHARMACY, DCLE UNVERSTY, 21280 DYARBAKIR
2
DCLE UNVERSTY, FACULTY OF PHARMACY DEPARTMENT OF PHARMACEUTCAL CHEMSTRY TR-
21280 DYARBAKIR
3
DEPARTMENT OF NUTRTON AND DETETCS, SCHOOL OF HEALTH, BATMAN UNVERSTY, 72060
BATMAN
4
DCLE UNVERSTY, FACULTY OF SCENCE, DEPARTMENT OF ANALYTCAL CHEMSTRY, TR-21280
DYARBAKIR, TURKEY
5
DEPARTMENT OF GENERAL AND ANALYTCAL CHEMSTRY, FACULTY OF PHARMACY, ISTANBUL
UNVERSTY, TURKEY

1013
 Poster Session 7
Submission ID: 932

THE ANTIBACTERIAL ACTIVITY OF LAUREL (LAURUS NOBILIS)

ESSENTIAL OILS AGAINST BACILLUS SUBTILIS

1 1 2
DENZ DAMLA ALTAN , TUNCAY GM , ERAY TULUKU

ABSTRACT
Medicinal and aromatic plants have been used since ancient times for their organoleptic
characteristics, therapeutic and medicinal properties. There has been an increased interest in
antimicrobial properties of essential oils and extracts from aromatic plants. Laurus nobilis L. is an
aromatic plant, frequently used as a spice in Mediterranean cookery. Laurel commonly known as
sweet bay, bay laurel, Grecian laurel, true bay, and bay. It belongs to Laureacea family and is native to
warm regions of the world, particularly in the Mediterranean countries (Turkey, Greece, Spain,
Portugal, Italy and France). The essential oil and extracts obtained from the leaves of laurel have been
used to fungal and bacterial infections, to treat epilepsy, parkinsonism, hemorrhoid and rheumatic
pains. Several studies show that essential oils of this plant can prolong the storage life of foods by
their antioxidant and antimicrobial activities. Ropiness is bacterial spoilage of bread that is commonly
caused by Bacillus spp., especially Bacillus subtilis. It is the most important spoilage of bread after
mouldiness which occurs particularly in summer and initially occurs as an unpleasant fruity odor,
followed by a discoloured, sticky and softbread crumb, caused by enzymatic degradation. The aims of
this study was to determine antibacterial activity of essential oils of laurel against Bacillus subtilis.
The essential oils were obtained from laurel leaves that collected from different locations of Turkey
(Izmir, Denizli, Antakya-Hatay) and purchased commercially, were experimented for their
antibacterial activity against Bacillus subtilis. Reference bacteria used in the study was Bacillus
subtilis ATCC 11774. Disc diffusion method was employed for the determination of antibacterial
activity of the essential oils and the inhibition zone diameters were measured in millimeters. The
essential oils of laurel (Izmir, Antakya-Hatay, Denizli and commercial) showed antibacterial activity
against tested reference bacteria Bacillus subtilis with different inhibition zones of 37.903.89,
17.500.43, 7.600.84 and 37.950.63 mm, respectively.

KEYWORDS
Laurel, Laurus nobilis L., Essential Oils, Bacillus subtilis, Antibacterial Activity

Corresponding Author: DENZ DAMLA ALTAN, ddaltan@nku.edu.tr

1
NAMIK KEMAL NVERSTES
2
SELUK NVERSTES

1014
 Poster Session 7
Submission ID: 933

ANTIOXIDANT ACTIVITIES OF THE EXTRACTS FROM DIFFERENT

PARTS OF ENDEMIC SALVIA CERINO-PRUINOSA VAR. CERINO-
PRUINOSA AND SALVIA ROSIFOLIA

1 1 2 3 4
ESRA YARI , ABDULSELAM ERTA , HATICE AKIRCA , MEHMET FIRAT , MEHMET BOA , MUSTAFA
5 6 5 7 8
ABDULLAH YILMAZ , MEHMET ZTRK , HAMDI TEMEL , UFUK KOLAK , GLATI TOPU

ABSTRACT
The genus Salvia, with about 900 species, is one of the most widespread members of the
Lamiaceae family plants. An unusually large number of useful secondary metabolites, belonging to
various chemical groups, such as terpenoids, flavonoids, and other compounds, have been isolated
from the genus, which features prominently in the pharmacopeias of many countries throughout the
world for wound healing and alleviating stomach, liver, and rheumatism pains and for treating the
common cold in the form of infusion. Some Salvia (Sage) species have been used as medicinal plants
to treat bronchitis, tuberculosis, menstrual and digestive disorders. They are also being used as spices
and tea throughout the world since ancient times. They posses antioxidant, antibacterial, antitumor,
cardioactive and antidiabetic activities. Antioxidant activities of Lamiaceae (Labiatae) plants which
are widely grown in Turkey were also investigated by our group. In this research, antioxidant activities
of extracts obtained from various parts of (roots, leaves, stems, flowers and mixed) Salvia cerino-
pruinosa var. cerino-pruinosa and Salvia rosifolia were compared (DPPH, Beta Caroten Cuprac,
ABTS). While petroleum ether and chloroform extracts of both species have shown low antioxidant
activity, ethanol extracts of both species has shown well antioxidant activity. When compairing both
species various parts each other, ethanol extracts of leaves of both species have shown high activity,
espacially. It can be said that both endemic Salvia species have potential of antioxidant.
Acknowledgements: The research was funded by grant : KBAG 114Z801 from TUBITAK, The
Scientific and Technological Research Council of Turkey.

KEYWORDS
Salvia cerino-pruinosa var. cerino-pruinosa, Salvia rosifolia, antioxidant.

Corresponding Author: HATCE AKIRCA, haticecakirca@windowslive.com

1
DEPARTMENT OF PHARMACOGNOSY, FACULTY OF PHARMACY, DCLE UNVERSTY, DYARBAKIR
2
DCLE UNVERSTY, FACULTY OF PHARMACY, DEPARTMENT OF ANALYTCAL CHEMSTRY,
DYARBAKR, TURKEY
3
DEPARTMENT OF BOLOGY, FACULTY OF EDUCATON, YZNC YIL UNVERSTY, VAN
4
DEPARTMENT OF PHARMACEUTCAL TECHNOLOGY, FACULTY OF PHARMACY, DCLE UNVERSTY,
DYARBAKIR
5
DCLE UNVERSTY, FACULTY OF PHARMACY DEPARTMENT OF PHARMACEUTCAL CHEMSTRY TR-
21280 DYARBAKIR
6
MUGLA SITKI KOMAN UNVERSTY, FACULTY OF SCENCE, DEPARTMENT OF CHEMSTRY, TR-48121
MUGLA
7
DEPARTMENT OF GENERAL AND ANALYTCAL CHEMSTRY, FACULTY OF PHARMACY, ISTANBUL
UNVERSTY,ISTANBUL
8
DEPARTMENT OF PHARMACOGNOSY AND PHYTOCHEMSTRY, BEZMALEM VAKF
UNVERSTY,ISTANBUL, TURKEY

1015
 Poster Session 7
Submission ID: 935

ENZYME INHIBITORY PROPERTIES OF DIFFERENT SOLVENT

EXTRACTS FROM COLUTEA CILICICA

1 2 1 1
ABDURRAHMAN AKTUMSEK , GOKALP OZMEN GULER , GOKHAN ZENGIN , RAMAZAN CEYLAN

ABSTRACT
Enzyme inhibitory properties of ethyl acetate, methanol, and water extracts form Colutea
cilicica (Fabaceae) were investigated with spectrophotometric methods. Enzyme inhibitory effect were
evaluated aganist cholinesterase, tyrosinase, -amylase and -glucosidase. The methanol extract
exhibited the strongest cholinesterase inhibitory activity with 1.326 mgGALAE/g extract in AChE and
0.682 mgGALAE/g extract in BChE. However, the water extract was not active on cholinesterases.
The best tyrosinase inhibitory effect were observed in the ethyl acetate extract with 54.70 mgKAE/g
extract. Similarly, the ethyl acetate extract exerted the strongest amylase and glucosidase inhibitory
effect. These findings suggest that the C. cilicica could serve as an important natural source of
biologically active agents for using in food and pharmaceutical industry. Acknowledgements: This
work was supported by The Scientific and Technological Research Council of Turkey (TUBITAK),
Turkey, Project No: 113Z892.

KEYWORDS
Colutea cilicica, enzyme inhibitory properties, natural products

Corresponding Author: GOKALP OZMEN GULER, gguler@konya.edu.tr

1
SELCUK UNVERSTY, SCENCE FACULTY, DEPARTMENT OF BOLOGY, KONYA, TURKEY
2
NECMETTN ERBAKAN UNVERSTY, AHMET KELESOGLU EDUCATON FACULTY, DEPARMENT OF
BOLOGCAL EDUCATON, KONYA, TURKEY

1016
 Poster Session 7
Submission ID: 936

ENZYME INHIBITORY EFFECTS OF DIFFERENT SOLVENT

EXTRACTS FROM EBENUS HIRSUTA

1 2 1 1
ABDURRAHMAN AKTUMSEK , GOKALP OZMEN GULER , GOKHAN ZENGIN , RAMAZAN CEYLAN

ABSTRACT
Enzyme inhibitory capacities of ethyl acetate and water extracts form Ebenus hirsuta
(Fabaceae) were investigated with spectrophotometric methods. Enzyme inhibitory effects were
evaluated aganist cholinesterase, tyrosinase, -amylase and -glucosidase. The ethyl acetate extract
has the highest cholinesterase inhibitory effects as compared to water extract. However, the water
extract exhibited the strongest anti-tyrosinase effect with 55.06 mgKAE/g extract. Also, the ethyl
acetate extract had the best anti-diabetic effects with the highest amylase inhibiton. Our findings
suggest that the Ebenus hirsuta could serve as an valuable source of natural enzyme inhibitors.
Acknowledgements: This work was supported by The Scientific and Technological Research Council
of Turkey (TUBITAK), Turkey, Project No: 113Z892.

KEYWORDS
Ebenus hirsuta, enzyme inhibitory effect, natural products

Corresponding Author: GOKALP OZMEN GULER, gguler@konya.edu.tr

1
SELCUK UNVERSTY, SCENCE FACULTY, DEPARTMENT OF BOLOGY, KONYA, TURKEY
2
NECMETTN ERBAKAN UNVERSTY, AHMET KELESOGLU EDUCATON FACULTY, DEPARMENT OF
BOLOGCAL EDUCATON, KONYA, TURKEY

1017
 Poster Session 7
Submission ID: 938

ANALYSIS OF MEDICINAL AND AROMATIC PLANTS

CONSUMPTION OF HOUSEHOLDS IN URBAN AREA: THE CASE OF
IZMIR-TURKEY

1 2 3 4 5
HLYA ARSLAN , SAIT ENGINDENIZ , GRKEM ZTRK , NDER VOLKAN BAYRAKTAR , DOAN ARSLAN

ABSTRACT
Medicinal and aromatic plants play a significant role in the life of people and are present in
innumerable forms. Medicinal and aromatic plants constitute a major segment of the flora, which
provides raw materials for use in the pharmaceuticals, cosmetics, and drug industries. The main aim of
this study is to analyze consumer structure, consumption trends and preferences with data obtained by
survey from hoseholds in urban area of Izmir. For this aim, 96 surveys by proportional sampling have
been carried out with members of households in center of Karyaka district of Izmir. Study was
carried out on hoseholds of dwellings located at six distinct streets of 16 quarters. As a results of the
study, a large part of household, medical and aromatic plants does not recognize under the name. In
their natural environment, the additivies include, delicious foods, and they have to be protective,
immune system booster due to the properties of the products are preferred. Consumer preferred the
kind, quantity and product production with the right group of consumers as a result of the
determination, the process from production to marketing for effectiveness and sustainability will be
provided.

KEYWORDS
medicinal and aromatic plant, consumer analysis, consumer attitudes and behaviors,
consumer preferences

Corresponding Author: GRKEM ZTRK, gorkem.cosar@tarim.gov.tr

1
T.C. ORMAN GENEL MDRL,ZMR ORMAN BLGE MDRL
2
EGE NVERSTES, ZRAAT FAKLTES, TARIM EKONOMS BLM
3
ZRA MCADELE ARATIRMA ENSTTS MDRL-BORNOVA
4
SRT NVERSTES, ZRAAT FAKLTES, TARIM EKONOMS BLM
5
SRT NVERSTES, ZRAAT FAKLTES,TARLA BTKLER BLM

1018
 Poster Session 7
Submission ID: 939

INVESTIGATION OF ANTIOXIDANT ACTIVITIES OF ESKTRAKTS

OBTAINED FROM LALLEMANTIA CANESCENS (L.) FISCH. & C.A.
MEY. AND LALLEMANTIA PELTATA (L.) FISCH. & C.A. MEY.
SPECIES

1 2 3 4
AHMET SAVCI , YUSUF ALAN , MURAT KURAT , SIRA TOPDEMR

D
TE
ABSTRACT
There are three species of Lallemantia (Lamiaceae) genus in Turkey. In our work, plant
material was collected in Bitlis province. The collected plant samples of Lallemantia canescens
(Topajadarba) and Lallemantia peltata (Kalkanba) were dried and extracted with the soxhalat

EN
method. The antioxidant activities of the extracts obtained were investigated. Antioxidant activities of
plant extracts were examined using different in vitro methodologies such as total antioxidant activity
by ferric thiocyanate, total reducing power by potassium ferricyanide reduction method, reduction
capacity of cupric ions (Cu2+) by the Kuprak method, 1,1-diphenyl-2-picryl-hydrazyl (DPPH) free
radical scavenging, 2,2-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical scavenging
ES
activity. Compared with the standard antioxidants BHA, BHT and -tocopherol, reduction capacities
for L.canescens and L. peltata plants are as follows; BHA > L.peltata > BHT > -tokoferol >
L.canescens. According to the results of the study,% inhibition was 70.77% for BHT, 66.35% for
BHT, 62.11% for -tocopherol and 61.86% for extraction. When we look at the results of ABTS+
PR

radical scavenging activity, we can say that all of them are close to each other and show very good
activity. ABTS radical activities of plants and standards are listed as follows: BHT (%96,16) -
tocopherol (%96,15) BHA (%96,04) L.peltata (%95,86) L.canescens (%95,50).

KEYWORDS
T

Lallementia canescens, Lallamentia peltata,Extract, Antioxidant Activity

O
N

Corresponding Author: AHMET SAVCI, a.savci@alparslan.edu.tr

1
MU ALPARSLAN NVERSTES, KMYA BLM, FEN EDEBYAT FAKLTES, 49100-MU /TRKYE
2
MU ALPARSLAN NVERSTES, TEMEL ETM BLM, ETM FAKLTES, 49100-MU /TRKYE
3
BTLS EREN NVERSTES, BYOLOJ BLM, FEN EDEBYAT FAKLTES, 13000-BTLS/TRKYE
4
BTLS EREN NVERSTES, BYOLOJ BLM, FEN BLMLER ENSTTS, 13000-BTLS/TRKYE

1019
 Poster Session 7
Submission ID: 940

ANTIOXIDANT AND ANTIINFLAMMATORY EFFECTS OF

CURCUMIN

1 2
EMEL AKTA , HILAL YILDIRAN

ABSTRACT
Throughout the history of humanity, plants have been used for treating many diseases. This
information, obtained through trial and error, has reached to day with some changes and developments
in the manner of use throughout the ages. Recently, interest in antioxidant and antiinflammatory
products and consumption of natural foods and nutritional bioactive components has been increasing
steadily. Curcumin is found in turmeric spice which is a popular member of the ginger family. It gives
the curry bright yellow color and is used as a herbal medicine in China and India for thousands of
years. In humans, curcumin is known to be safe, but not toxic, and turmeric is classified as an additive
in the E100 category. Curcumin has been reported to have various pharmacological properties such as
antimicrobial, antiviral, antifungal, anticarcinogenic and wound healing. However, it has radical
scavenger, iron chelator and antiinflammatory properties in different tissues. Antiinflammatory and
antioxidant properties are two important mechanisms underlying the majority of the pharmacological
effects of curcumin.

KEYWORDS
curcumin, antioxidant, antiinflammatory

Corresponding Author: EMEL AKTA, emelaktas@karabuk.edu.tr

1
KARABK NVERSTES SALIK BLMLER FAKLTES, BESLENME VE DYETETK ANABLM DALI
2
GAZ NVERSTES SALIK BLMLER FAKLTES, BESLENME VE DYETETK ANABLM DALI

1020
 Poster Session 7
Submission ID: 941

INVESTIGATION OF ANTIOXIDAN ACTIVITIES OF EXTRACTS

OBTAINED FROM NEPETA TRANSCAUCASICA GROSSH. VE
SCUTELLARIA ALBIDA L. SUBSP. CONDENSATA (RECH.F.)
J.R.EDM. TAXA

1 2 3 4
AHMET SAVCI , YUSUF ALAN , MURAT KURAT , SIRA TOPDEMR

D
TE
ABSTRACT
Lamiaceae family in Turkey is represented by 46 genera, 577 species and 755 taxa in total. In
our work, plant material was collected in Bitlis province. The collected plant samples of Nepeta
transcaucasica (Kaf pisikotu) and Scutellaria albida L. subsp. condensata (Krk kaside) were dried and

EN
extracted with the soxhalat method. The antimicrobial activities of the extracts obtained were
investigated. Antioxidant activities of plant extracts were examined using different in vitro
methodologies such as total antioxidant activity by ferric thiocyanate, total reducing power by
potassium ferricyanide reduction method, reduction capacity of cupric ions (Cu2+) by the Kuprak
method, 1,1-diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenging, 2,2-azino-bis(3-
ES
ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical scavenging activity. Compared with the standard
antioxidants BHA, BHT and -tocopherol, reduction capacities for L.canescens and L. peltata plants
are as follows; BHA > BHT > -tokoferol > N. transcaucasica > S. albida subsp. condensata.
According to the results of the study,% inhibition was 70.77% for BHT, 66.35% for BHT, 62.11% for
-tocopherol and 61.86% for extraction. When we look at the results of ABTS+ radical scavenging
PR

activity, we can say that all of them are close to each other and show very good activity. ABTS radical
activities of plants and standards are listed as follows: BHT (%96,16) -tocopherol (%96,15) BHA
(%96,04) S.albida subsp. condensata (%96,04) N. transcaucasica (%95,98).
T

KEYWORDS
O

Nepeta transcaucasica, Scutellaria albida L. subsp. condensata,Extract, Antioxidant activity

N

Corresponding Author: AHMET SAVCI, a.savci@alparslan.edu.tr

1
MU ALPARSLAN NVERSTES, KMYA BLM, FEN EDEBYAT FAKLTES, 49100-MU /TRKYE
2
MU ALPARSLAN NVERSTES, TEMEL ETM BLM, ETM FAKLTES, 49100-MU /TRKYE
3
BTLS EREN NVERSTES, BYOLOJ BLM, FEN EDEBYAT FAKLTES, 13000-BTLS/TRKYE
4
BTLS EREN NVERSTES, BYOLOJ BLM, FEN BLMLER ENSTTS, 13000-BTLS/TRKYE

1021
 Poster Session 7
Submission ID: 943

THE ANTIOXIDANT ACTIVITIES OF THE ESSENTIAL OILS OF

SOME SALVIA SPECIES FROM TURKEY

1 2 1 3 4 5
HATICE AKIRCA , ABDULSELAM ERTA , IIL AYDIN , MEHMET FIRAT , MEHMET BOA , SERKAN YIITKAN ,
5 6 1 7
HASAN AHIN , NESRIN HAIMI , FIRAT AYDIN , GLATI TOPU

ABSTRACT
The Salvia L. genus belongs to the subfamily Nepetoideae in Lamiaceae family. The genus
consists of about 900 species. Many Salvia species are used as herbal tea and for food flavoring, as
well as in cosmetic, perfumery and the pharmaceutical industries throughout World. Salvia species are
generally known for their multiple pharmacological effects including their antibacterial, antiviral,
antioxidative, antimalarial, anti-inflammatory, antidiabetic, cardiovascular, antitumor and anticancer
activities. Also, some studies showed that a part of these activities depended on their essential oil
composition. The essential oils of four Salvia species were tested for antioxidant (-Carotene-linoleic
acid test system, DPPH free radical scavenging activity, ABTS cation radical decolorisation and
cupric reducing antioxidant capacity) activities in this study. Essential oil samples were obtained by a
Clevenger apparatus from the whole parts of plants which were crumbled into small pieces and soaked
in distilled water for 3 h. Then, these samples were dried over anhydrous Na2SO4 and stored at +4C
for a sufficient period of time. The antioxidant activities of essential oils of four Salvia species were
found to be medium and low, generally. The essential oils of S. multicaulis and S. montbretii showed
good activity (IC50: 436,63236,32 and 374.75334,97, respectively) with ABTS cation radical
decolorisation method.

KEYWORDS
Salvia multicaulis, Salvia pinnata, Salvia spinosa, Salvia montbretii, Essential Oil,
Antioksidant

Corresponding Author: HATCE AKIRCA, haticecakirca@windowslive.com

1
DCLE UNVERSTY, FACULTY OF PHARMACY, DEPARTMENT OF ANALYTCAL CHEMSTRY,
DYARBAKR, TURKEY
2
DCLE UNVERSTY, FACULTY OF PHARMACY, DEPARTMENT OF PHARMACOGNOSY, DYARBAKIR,
TURKEY
3
YZNC YIL UNVERSTY, FACULTY OF EDUCATON, DEPARTMENT OF BOLOGY, VAN, TURKEY
4
DCLE UNVERSTY, FACULTY OF PHARMACY, DEPARTMENT OF PHARMACEUTCAL TECHNOLOGY,
DYARBAKIR, TURKEY
5
DCLE UNVERSTY, FACULTY OF PHARMACY, DEPARTMENT OF PHARMACOGNOSY, DYARBAKIR,
TURKEY
6
DEPARTMENT OF NUTRTON AND DETETCS, SCHOOL OF HEALTH, BATMAN UNVERSTY, 72060
BATMAN
7
STANBUL UNVERSTY, FACULTY OF PHARMACY, DEPARTMENT OF ANALYTCAL CHEMSTRY,
STANBUL, TURKEY

1022
 Poster Session 7
Submission ID: 946

A HEALING HERB IN KARSNIYACINCAR

1 1 1
LALE SARIYE AKAN , YAHYA ZDOAN , RAHIME EVRA KARAKAYA

ABSTRACT
Ardanu is a district which had hosted rooted and rich civilizations. Karsniya village of
Ardanu is located in eastern of Black Sea, left slope of oruh valley where is founded neolithic age
human evidence BC 10-8 thousand years. Excavations exposing metallic and especially various
kitchen equipment demonstrate that prehistorical ages are lived respectively.Karsniya which is a high
mountain village is not convenient for agriculture and farming. Not only vegetable, fruit and cereal
production but also farming is made traditional procedures in Karsniya and the obtained products are
not satisfactory. Vegetable requirement in Karsniya is obtained from herbs called greens.The main
herbs that provides emerging a rich nutrition style and grows itself in nature are: cincar herb, flutter
herb, gumi herb, crowbar, gelin parmagi, pampara and sorrel. The aim of this study is to present
various herbs and declare benefits of cincar soup and its ingredients and making processes which is
made of cincar herb which is believed to be healing. Cincar herb blooms in may- october, reachs 20-60
cm, is located in bottom of walls and roadsides and causes severe blushing and itching when leaves are
exposed to skin. Herbs roots are dried up in spring and autumn, leaves are dried up in may and july,
seeds are dried up in july and august by collecting. Even though the belief of herbs healing property is
came across in Middle Europe, the women in Karsniya observed that touching this herbs heals skin
wounds, boiling the herbs water inhibits hair loss and consuming the herb cures stomach and
intestinal diseases.As a conclusion it is detected that cincar herbs is used in various meals and
especially cincar soup is believed to be a healing meal by village women. Recording making processes
of cincar soup is considered to bring Turkish Culture richness by generalizing formation of it.

KEYWORDS
Cincar soup, healing herb, kitchen culture, Karsniya

Corresponding Author: RAHME EVRA KARAKAYA, recakir@ybu.edu.tr

1
ANKARA YILDIRIM BEYAZIT NVERSTES SALIK BLMLER FAKLTES BESLENME VE DYETETK
BLM

1023
 Poster Session 7
Submission ID: 948

CYTOTOXIC EFFECTS OF NATURALLY OCCURRING

FLAVONOIDS ON HUMAN COLON CANCER CELLS

1 2 3
SERDAR KARAKURT , IDEM GKEK SARA , SEVTAP KARAKURT

ABSTRACT
Colon cancer is the third leading disease of death in the world. The resistance against drugs
used in colon cancer treatment and elevated side effects of those drugs leads investigators to find new
strategies. Up to now, about 500 different polyphenols have been isolated and reported in foods and
beverages of plant origin. In this study, we focused on to investigate effects of quercetin (Q) and rutin
(R) against human colon cancer cells. DLD1 cells and HT-29 cells were grown in Leibowitzs L-15
medium and McCoy's 5a medium respectively supplemented with 10% fetal bovine serum and 2 mM
glutamine. Optimum cell number and growth time of cells were determined spectrophotometrically
and effect of Q and R on the viability of DLD1 cells and HT-29 cells were determined with Alamar
blue and IC50 values were calculated from the sigmoidal graph. The IC50 values of Q were calculated
as 144 M and 44 M in DLD-1 and HT-29 cells, respectively. On the other hand, R was found less
toxic on both cancer cells with IC50 of 213 M and 552 M for DLD1 cells and HT-29 cells,
respectively. The data obtained from this study will supply valuable information about the usage of
these flavonoids in the treatment of colon cancer since quercetin and rutin are found plenty amounts in
vegetables and fruits.

KEYWORDS
Colon Cancer, Cytotoxicity, Proliferation, Quercetin, Rutin.

Corresponding Author: SERDAR KARAKURT, kserdar1@yahoo.com

1
DEPARTMENT OF BOCHEMSTRY, FACULTY OF SCENCE, SELCUK UNVERSTY
2
DEPARTMENT OF BOMEDCAL ENGNEERNG, FACULTY OF ENGNEERNG, AKDENZ UNVERSTY
3
FACULTY OF ENGNEERNG, CHEMCAL ENGNEERNG DEPARTMENT, SELCUK UNVERSTY

1024
 Poster Session 7
Submission ID: 949

A CURE ALL ST.JOHNS WORT

1 1 1
RAHIME EVRA KARAKAYA , LALE SARIYE AKAN , YAHYA ZDOAN

ABSTRACT
St.Johns wort is a herb that grows by itself in nature and it is believed to be healing as its
usage area varies progressively.The aim of this study is to specify St.Johns Worts properties, usage
areas and side affects. This herb is also called as kanotu, yaraotu, klotu, mayaslotu, binbirdelik
otu among public and its flower and roots are used for benefit. St.Johns Wort includes tannin,
volatile oils, flavon species (rutin, guercitin, guercitrin), hipericin (hypericin), hyperin (substance that
gives colour to herb), caroten, bitter materials, rosin, pectin and colic, gum, vitamin c and resin as
substances. This herb is usually consumed as tea or its oil is used externally. According to studies
accomplished with this herb, quick recovery is observed with depression, lack of attention, bone
diseases, irritabl bowel syndrome, wound healing, skin diseases and burn. It is reported that long term
usage of this herb as treatment might cause unfavourable effects on health. This herbs content and
dosage may differ due to its vegetative region. Although St.Johns Wort is known with various
benefits, in order to generalize its usage areas it is thought that more studies might be beneficial about
its habitat, dosage level, duration and side effects.

KEYWORDS
St. Johns wort (Hypericum perforatum), health, herb

Corresponding Author: RAHME EVRA KARAKAYA, recakir@ybu.edu.tr

1
ANKARA YILDIRIM BEYAZIT NVERSTES SALIK BLMLER FAKLTES BESLENME VE DYETETK
BLM

1025
 Poster Session 7
Submission ID: 950

THE USE OF ESSENTIAL OILS AS ANTIMICROBIAL SUBSTANCES

IN SEAFOOD

1 1 2 1 1
HATICE GNDZ , FATMA ZTRK , SEVIM HAMZAEBI , ASENA NVER , M. DILCAN AKPINAR

ABSTRACT
Seafood is a food product that is rapidly spoiled due to microbial activity during cold storage.
This is an important issue both economically and in terms of human health. There are several methods
in traditional and long-term conservation to prevent or delay the growth of pathogenic and spoiled
microorganisms during the storage of seafood. In addition, the storage period can be extended by
adding antimicrobial or antibacterial agent to seafood. These substances added to protect of foods can
be synthetic or natural additives. Today, consumers are choosing to reduce the use of synthetic
chemicals in order to increase the shelf life of foods. Essential oils, which offer an alternative to
chemical use, are natural antimicrobials prolonged shelf-life of seafoods and can be used alone or in
combination with other preservation methods. Volatile oils, also called essential oils, are obtained
from plants or parts of these plants by distillation or pressing. It has been revealed by researchers that
essential oils with antimicrobial and antiseptic properties are effective on microorganisms cause
deterioration and poisoning in food. Phenolic compounds in volatile oils cause sensitization of the
phospholipid layer in cell membranes and increase the permeability of this membrane. Thus, they
inhibit microorganisms by causing intracellular components to leak out of the cell or to degrade
enzyme systems. The composition, structure and functional groups of essential oils play an important
role in the effectiveness of these antimicrobial activities. The oils of plants such as carnations, thyme,
rosemary and sage are the most effective oils against microorganisms. Antimicrobial effects of thyme,
oregono, lemon, rosemary, laurel, clove, orange and mandarin peel essential oils have been
investigated in seafood. In this study, the use of essential oils as antimicrobial substances in seafood
will be compiled.

KEYWORDS
Essential oils, Seafood, Antimicrobial, Shelf life, Food additive

Corresponding Author: HATCE GNDZ, hatice.gunduz1@ikc.edu.tr

1
ZMR KATP ELEB NVERSTES
2
FATMA.OZTURK@KC.EDU.TR

1026
 Poster Session 7
Submission ID: 951

NUTRITIONAL QUALITY AND HEALTH BENEFITS OF LUPIN

1 1 1 1
BURCU DENIZ GNE , HATICE BLKBAI , GAMZE AKBULUT , NILFER ACAR TEK

ABSTRACT
Legumes are important especially in regions where the economic status is not good due to the
fact that it can be obtained more and cheaper. Lupin, which belongs to the Leguminosae (or Fabaceae)
family, includes 450 Lupinus species. Only four species are cultivated; Lupinus albus (white lupin),
Lupinus luteus (yellow lupin), Lupinus angustifolius (blue lupin, narrow-leafed lupin), and Lupinus
mutabilis (tarwi, Andean lupin). Lupin is used as soy substitute, gluten free flour, emulsifier, a bread,
biscuits, cakes, pasta and snack in the world. The lupine, which has 2-3 times more protein than
grains, is also rich in vitamins and minerals such as calcium and iron. L. mutabilis is rich in complex
oligosaccharides, fiber and omega 3 fatty acids. Lupin flour increase the nutritional quality and
potential health benefits of bread by increasing protein, dietary fiber and carotenoid content, levels of
the protein -conglutin. Consuming lupin compared to wheat bread and other baked products reduce
chronic disease risk markers. Protein -conglutin improves glucose transport and elevates pancreatic
insulin content. Lupin have positive effects on hyperglycemia and insulin release. Furthermore,
treatment with lupin improved insulin resistance in subjects with glucose abnormalities. Lupin shows
hypolipidemic effect in individuals who have diet-induced hypercholesterolemia, by decreasing in
plasma total cholesterol, trigliserid, very low density lipoprotein (VLDL-C) and low density
lipoprotein (LDL-C). Increased dietary fibre may have a favourable influence on blood pressure
decrease. Lupin has also positive effects on the energy balance by suppressing appetite. Higher satiety
and lower energy intake of Lupin affects appetite-regulating hormone called ghrelin. However; long
term effects on energy intake and body weight in obese is not clear yet. Lupin and their products may
effects on human body possitively. Therefore, they begin to offer in shops with other legume species.
Consequently; increases in lupin products in the diet may have a beneficial role in chronic diseases,
but additional data from human intervention studies are needed.

KEYWORDS
hypercholesterolemia, insulin resistance, lupin

Corresponding Author: HATCE BLKBAI, hatice_urf@hotmail.com

1
GAZ NVERSTES SALIK BLMLER FAKLTES BESLENME VE DYETETK BLM

1027
 Poster Session 7
Submission ID: 952

EFFICIENCY OF A HERBAL LIQUID EXTRACT MIXTURE FOR

PREVENTING OF SALMONELLA GROWTH IN WHIPPED CREAM

1 1
ZM ZOLU , EVRIM GNE ALTUNTA

ABSTRACT
Pastry products with cream are commonly used in the food and confectionery industries1.
Desserts and cakes containing milk or cream which are milk-based products are suitable media with
high nutrient content for microbial growth1,2. Besides, pastry creams tend to contamination with
several pathogenic microorganisms because of their ingredients and production methods like using
raw cream1,2,3,4,5. Therefore; pastry cream is the main cause of food poisoning among humans1.
Salmonella is one of the major pathogenic microorganisms in the pastry cream1,3,4. The most
common recorded cause of diarrheal illnesses is Salmonella contamination in industrialized
countries5. In addition to that, the Ministry of Health in Italy was reported that Salmonella was the
most important reason of the outbreaks6. Salmonella is found in the environment such as water, soil,
sewage and gastrointestinal tract of animals and humans,3,7. Meat, poultry, fish, egg, milk, dairy
products, fruits and vegetables are transmission vector of Salmonella3,5,7,8,9,10. While Salmonella
infection (Salmonellosis) is prevented with lots of different methods, including adding antimicrobial
or antibiotic compounds8,9, last studies have been focused on using spice liquid extract for preventing
Salmonella growth11,12,13,14,15. In this study, a herbal liquid extract mixture containing sorrel
extract (Rumex acetosella), millfoil extract (Achillea millefolium), ribwort plantain extract (Plantago
lanceolata) (ASATM ST 1412; recommended for milk-based product and whipped cream by
company, Kayseri, Turkey) was used to exhibit the inhibitory effect on Salmonella Enteritidis ATCC
13076 growth in whipped cream (prepared with milk as the company proposed). For this aim, 8
different groups were designed which were only pastry cream as K1, cream with Salmonella as K2,
cream added herbal liquid extract mixture at 0.1%, 1% and 10% ratio as K3, K4, K5 respectively,
cream added Salmonella and herbal liquid extract mixture of 0.1%, 1%, 10% to the samples were left
1 to 3 hour at room temperature. Then, these samples were inoculated to Plate Count Agar in two
parallel for each group with regard to Dropping Plate Technique at the end of the 1st, 2nd and 3rd
hours. The inoculation amount of Salmonella was selected as 0.4 (O.D.600) which equal to 106cfu/ml.
After inoculation, the plates were incubated at 37 C for 24 hours and then colonies were counted. As a
result of this study, number of the bacteria of K1 was calculated as 4x104cfu/g at the end of 1 hour
and then 2 logarithmic unit increasing was observed and the colony number has reached to
1.7x107cfu/g, at the end of 3rd hour. This means that, whipped cream has microbial risk when it is
waited in room temperature. The other remarkable result was observed in the sample containing 10%
herbal liquid extract mixture and 106cfu/g Salmonella as well. The amount of bacteria was calculated
as 3.8x104cfu/g, 2.68x105cfu/g and 1.3x105cfu/g at the end of the 1st, 2nd and 3rd hours respectively.
Thus, it can be reported that 10% ratio of herbal liquid extract mixture in whipped cream has an
inhibitory effect on the bacteria when the result compared with the control sample. The producing

Corresponding Author: ZM ZOLU, ozum.ozoglu@gmail.com

1
ANKARA NVERSTES BYOTEKNOLOJ ENSTTS

1028
company of the herbal liquid extract mixture proposes that the herbal liquid extract mixture should be
added from 0.2% to 1% ratio to milk-based product and whipped cream. This approach is acceptable,
so that in this study, high level of Salmonella was inoculated to the samples at the beginning which is
an unusual situation. In conclusion, the study has shown that the herbal liquid extract mixture is an
alternative and useful method for precluding Salmonella growth in whipped cream. REFERENCES 1.
Sharifzadeh A, Hajsharifi-Shahreza M, Ghasemi-Dehkordi P. Evaluation of Microbial Contamination
and Chemical Qualities of Cream-filled Pastries in Confectioneries of Chaharmahal Va Bakhtiari
Province (Southwestern Iran). Osong Public Heal Res Perspect. 2016;7(6):346-350.
doi:10.1016/j.phrp.2016.09.004. 2. Al M, Sancak YC, Akkaya L, Bol CEL. Baz Stl Tatl lar n
Mikrobiyolojik Kalitelerinin Belirlenmesi *. 2002;26:975-982. 3. Ray B. Fundamental Food.;2004
http://books.google.com/books?hl=en&amp;lr=&amp;id=zYPFZby2wtcC&amp;oi=fnd&amp;pg=PA
1&amp;dq=Fundamental+Food+Microbiology&amp;ots=qvHkiBdUCb&amp;sig=WtdhhPDq1al7P_S
_jcef1xLo9t8.. 4. Hamedan EF. Microbial Contamination of Pastry Cream: 2016;5(3):207-213. 5.
Kotzekidou P. Microbiological examination of ready-to-eat foods and ready-to-bake frozen pastries
from university canteens. Food Microbiol. 2013;34(2):337-343. doi:10.1016/j.fm.2013.01.005. 6. team
EC for DP and C (ECDC)-HCU-E editorial. An outbreak of Salmonella enteritidis infection associated
with iced cake. 1999. http://www.eurosurveillance.org/ViewArticle.aspx?ArticleId=75. Accessed
March 26, 2017. 7. Mahmoud BSM. Salmonella - A Dangerous Foodborne Pathogen.; 2012.
doi:10.5772/1308. 8. Paio FG, Arisitides LGA, Murate LS, Vilas-Bas GT, Vilas-Boas LA,
Shimokomaki M. Detection of Salmonella spp, Salmonella Enteritidis and Typhimurium in naturally
infected broiler chickens by a multiplex PCR-based assay. Brazilian J Microbiol. 2013;44(1):37-41.
doi:10.1590/S1517-83822013005000002. 9. Kavaz Yksel A, Yksel M. Determination of Certain
Microbiological Quality Characteristics of Ice Cream, Detection of Salmonella by Conventional and
Immunomagnetic Separation Methods and Antibiotic Susceptibility of Salmnella spp. Isolates. J Food
Saf. 2015;35:385-394. doi:10.1111/jfs.12186. 10. DG, Koopmans M, Verhoef L, et al. Food-borne
diseases - The challenges of 20years ago still persist while new Newell ones continue to emerge. Int J
Food Microbiol. 2010;139(SUPPL. 1):S3-S15. doi:10.1016/j.ijfoodmicro.2010.01.021. 11. Amrutha
B, Sundar K, Shetty PH. Spice oil nanoemulsions: Potential natural inhibitors against pathogenic E.
coli and Salmonella spp. from fresh fruits and vegetables. LWT - Food Sci Technol. 2017;79:152-159.
doi:10.1016/j.lwt.2017.01.031. 12. Bernbom N, Ng YY, Paludan-Mller C, Gram L. Survival and
growth of Salmonella and Vibrio in som-fak, a Thai low-salt garlic containing fermented fish product.
Int J Food Microbiol. 2009;134(3):223-229. doi:10.1016/j.ijfoodmicro.2009.06.012. 13. Mahgoub SA,
Ramadan MF, El-Zahar KM. Cold Pressed N igella sativa Oil Inhibits the Growth of Foodborne
Pathogens and Improves the Quality of Domiati Cheese. J Food Saf. 2013;33(4):470-480.
doi:10.1111/jfs.12078. 14. Perumalla AVS, Hettiarachchy NS. Green tea and grape seed extracts
Potential applications in food safety and quality. Food Res Int. 2011;44(4):827-839.
doi:10.1016/j.foodres.2011.01.022. 15. Sanchez C, Batlle R, Nerin C. Enhanced antimicrobial vapour-
phase effect of natural extracts in active packaging. Is total protection reached?
http://i3a.unizar.es/datos/publicacion/enhanced-antimicrobial-vapour-phase-effect-of-natural-extracts-
in-active-packaging.-is-total-protection-reached.-20889?idioma=en. Published 2006. Accessed March
26, 2017.

KEYWORDS
Salmonella, whipped cream, herbal liquid extract mixture

1029
 Poster Session 7
Submission ID: 953

PROBIOTICS: THE EFFECTS ON GUT MICROBIOTA AND OBESITY

1 1 1 1
HATICE BLKBAI , GAMZE AKBULUT , NILFER ACAR TEK , YELIZ SERIN

ABSTRACT
Pathogenic and non-pathogenic microorganisms are in balance in healthy individuals. When
this balance deteriorates, the barrier-building function of the mucosa deteriorates and inflammation
begins. The changes in the microbiota increase the intestinal permeability and alter the functions of the
brain, pancreas, liver, muscle and fat tissue. As the permeability increases, there is also an increase in
the level of plasma lipopolysaccharides, which leads to rise in inflammatory cytokines. The risk of
metabolic disease increase due to inflammation. In obese individuals with insulin resistance there is
also an increase in the ratio of firmicutes / bacteroides on the basis of changes in the intestinal
microbiota. Diet is one of the most important factors contributing to the composition and diversity of
the intestinal microbiota. It is accepted in the society to think of protecting and improving the
healthiness of the foods other than providing the metabolic requirements. The use of probiotics is
increasing by the determination of the importance of the microbiota. According to the Food and
Agricultural Organization of the United Nations and the World Health Organization, probiotics are
defined as living microorganisms, which when administered in adequate amounts confer health
benefits on the host. Several different species of bacteria are used as probiotics. The most common
species are Bifidobacterium (adolescentis, animalis, bifidum, breve and longum) or Lactobacillus
(acidophilus, casei, fermentum, gasseri, johnsonii, paracasei, plantarum, rhamnosus and salivarius).
Antimicrobial molecules produced by the probiotic inhibits growth of pathogenic microorganisms.
Metabolites such as short-chain fatty acids produced by probiotics are found to inhibit the growth of
bacterial pathogens as a result of lowering intestinal pH. Many Lactobacilli strains increase barrier
function by increasing mucus layer and increase barrier function by preventing apoptosis of intestinal
epithelial cells. Probiotic Lactobacillus strains enhance the integrity of the intestinal barrier, decreased
translocation of bacteria across the intestinal mucosa. The lowest amount needed for beneficial effects
probiotic in humans remains is unknown. Generally the lowest proposed dose is 10 6-107cfu/mL ;
suffcient dose is 10 7-108cfu/mL. Probiotics may be beneficial in the prevention and treatment of
obesity by enhance microbiota. Recently it is indicated that 8 to 12 weeks of probiotic
supplementation may confer benefits such as decreased body weight, body mass index (BMI), as well
as decreased body weight gain, fat accumulation, and prevention of insulin resistance. Anti-obesity
effects of probiotics are the regulation of lipid and glucose metabolism, reduction of adipose cell size
and inflammation in adipose tissue, and reduction of inflammation in the liver. Probiotics is also
gaining wide attention because of increasing evidence of the role of gut microbiota.

KEYWORDS
Probiotics, Gut Microbiota, Obesity

Corresponding Author: HATCE BLKBAI, hatice_urf@hotmail.com

1
GAZ NVERSTES SALIK BLMLER FAKLTES BESLENME VE DYETETK BLM

1030
 Poster Session 7
Submission ID: 954

FUNCTIONAL CHARACTERISTICS AND PHYSIOLOGICAL

EFFECTS OF LEGUMES

1 1
ZELIHA NIRAK , MNIR ANIL

ABSTRACT
For many years legumes have been regarded as high nutrients because of their high protein
content and the many functional components they contain. In the world, 22% of plant proteins and 7%
of carbohydrates in human nutrition are provided from the edible legumes. Legumes, which have very
high nutritional values, are rich in basic amino acids such as lysine, threonine, leucine, phenylalanine,
tryptophan and aspartic acid. At the same time the digestibility of legume proteins is between 70-94%,
which is quite high. The fat content of legumes being generally low and often from polyunsaturated
fatty acids, especially linoleic acid (omega-6) increase nutritional value. The linoleic acid contained
plays a role in regulating the physiology of reproduction and lactation, the cholesterol distributor in
arteriosclerosis, the regulation of cardiovascular system, vision systems and mental activities, and the
prevention of the formation of nitrosamines in nitrate-nitrite poisoning. In addition, soluble raffinos,
which are carbohydrates found in legumes, are prebiotics that support the development of probiotics
selectively and progressively to the colonic without digestion. These substances support the
development of beneficial bacteria in the field, especially Bifidobacterium species. Since these
carbohydrates can not be digested in the small intestine, they are fermented by colon microflora in the
large intestine. The products of fermentation products are gas and short chain fatty acids. The resulting
short chain fatty acids promote beneficial colon mucosal health and have beneficial physiological
effects. Phenolic compounds, which are important compounds in legumes, also have important effects
on human health. Of these compounds, especially isoflavones have biological properties, including
broad-spectrum protection against hormones associated with certain types of cancer. Nutritional
isoflavones are known to protect against the oxidation of LDL particles. At the same time, isoflavones
have protective properties against osteoporosis by regulating calcium exchange on the cell membrane.
It is known that isoflavones inhibit the development of many diseases such as cancer, atherosclerosis,
diabetes and chronic inflammation, which act as antioxidants besides these effects and inhibit free
radical formation and accumulate in the organism. The most important nutritional factors in legumes
are nutritional fibers in the organic structure that come from the colon without being digested. While
nutritional fibersreduce sugar,lipid and cholesterol content in blood, it also shortens the duration of
solid waste, allowing rapid release of toxic substances from the colon. Often monosaccharides and
sugar acids (mannuronic, galacturonic, glucuronicand 4-o-methyl glucuronic acid) are the major
compounds that form nutritional fibers. The nutritional fibers in legumes have many important
physicochemical properties such as solubility, water and oil retention capacity, ion exchange capacity.
It is known that the fiber in the legume plays a role in strengthening the colonic function and lowers
the level of cholesterol in the high level fiber. Legumes are also protective against many diseases that
can be overcome by using dietary treatments such as diverticular diseases, colon and rectal cancers,
appendicitis, varicose veins and hemorrhoids, coronary heart diseases, gall stones and diabetes.The

Corresponding Author: ZELHA NIRAK, oncirakzeliha94@gmail.com

1
ONDOKUZ MAYIS NVERSTES

1031
physiological effects of nutritional fiber and the benefits of health are stated to be shortening transit
time and increasing the amount of waste, binding bile acids, breaking up into short-chain fatty acids in
the large intestine, increasing viscosity, slowing digestion and absorption. In addition, clinical trials
have shown that postprandial blood sugar, insulin levels, lipid levels in blood serum are reduced,
which is useful for the second type of diabetes. Therefore, withthedissemination of legumes
consumed, society will be fed healthy and as a result, treatment expenditures and labor loss will be
reduced and comfort periods in human life will be increased. For this purpose, it is necessary to
increase the production of legumes and to expand the usage areas of the food industry.

KEYWORDS
Legume, nutritional fiber, functional property, prebiotic

1032
 Poster Session 7
Submission ID: 957

THE ANTIOXIDANT AND ANTICARSINOGENESIS EFFECT OF

ROSEMARY

1 1 1 1
HATICE BLKBAI , GAMZE AKBULUT , NILFER ACAR TEK , YELIZ SERIN

ABSTRACT
Antioxidants; have protective effects against the oxidation that occur during the preparation
and consumption of foods and maintain product quality. The determination of the toxic effects of
synthetic antioxidants on human health, the use of herbal products began to gain importance. It has
been highlighted on the use of aromatic plants rosemary that has antioxidant activity due to essential
fatty acids. Rosemary (Rosmarinus officinalis L.) from the Laminacae (Labiatae) family is an
important medicinal and aromatic plant species. The aromatic plant rosemary has been used for
medicinal purposes due to its antioxidant, antiinflammatory, chemoprotective and antiadipogenic
affects. It is also known that rosemary extracts have antioxidant activity similar of synthetic
antioxidants. The potent antioxidant properties more than 90% of rosemary have been mainly
attributed to its major compounds; rosmanol, carnosol and carnosic acid. Rosemary extracts highest
antioxidant properties are also due to the presence of phenolic diterpenes. Terpenes are recognized for
various benefits expecially in the prevention and treatment of a wide range of cancer types. Rosemary
extract is also modulate the alteration of signaling pathways and molecules directly related to tumor
initiation and development. Rosmanol acts as a strong anti-inflammatory agent and inhibite tumor
development. Carnosol may induce apoptosis through the intrinsic pathway; purified carnosol and
carnosic acid are powerful inhibitors of lipid peroxidation in microsomal and liposomal systems.
Carnosol and carnosic acid could contribute to the chemopreventive, antitumoral and antimetastatic
activities of rosemary extracts . Oral administration of carnosol is well tolerated and mean intake is
estimated to be between 500 and 1500 mg/ day of carnosol and carnosic acid for adults. Rosemary
extract is a potential may be included in the anti-cancer diet. It is suggested that carnosic acid alone or
with the anticancer drugs preferable as a good practise for the treatment of many typies of cancers that
are resistant to chemotherapy.

KEYWORDS
Antioxidant, Carnosol, Rosemary

Corresponding Author: HATCE BLKBAI, hatice_urf@hotmail.com

1
GAZ NVERSTES SALIK BLMLER FAKLTES BESLENME VE DYETETK BLM

1033
 Poster Session 7
Submission ID: 958

HEALTH EFFECTS OF NUTS

1 1 1 1 1
OSMAN BOZKURT , NILFER ACAR TEK , GAMZE AKBULUT , BURCU DENZ GNE , GAMZE YURTTA

ABSTRACT
Nuts are on the diet of human beings since pre-agriculture times. The word "Nut" is rooted in
the words "nutrient, nutriment, nutrition" which are also nutritional and nutritious. Today the most
consumed nuts are almond, hazelnut, walnut and pistachio. Nuts are rich sources of saturated,
unsaturated fatty acids (MUFA, PUFA), vegetable protein, fibre, photosterols, polyphenols, vitamins
and minerals. Nuts are also rich in vitamins and minerals such as niacin, B6, folic acid, magnesium,
zinc, copper and potassium. The Adventist Health Study 1992 found that the consumption of nuts,
reduced the risk of coronary heart diseases. With these study, the number of studies on the health
effects of nuts has increased steadily. However, four points have to be noted regarding the
consumption of nuts. They may lead to possible weight gain, increase fat tissue in the body, cause
metabolic complications such as metabolic syndrome and diabetes, and allergic reactions. The idea
that nuts have high energy content and that the consumption of these foods can cause obesity are the
negative perception about nuts. However, studies have reported that consuming a certain amount of
nuts within the diet helps body to lose weight. In one study, the participants who consumed nuts had
lower BMI and waist circumference than those who did not consume or consumed less nuts. In
addition, nuts play a role in the regulation of blood sugar because of its rich magnesium content. In
one study, the prevalence of diabetes was found to be 25% lower in women consuming nuts more than
5 days a week than those who never consumed. In another study, a decrease in HbA1C levels was
observed in those who consumed 28 g nuts 5 days a week for 12 weeks. In a different study, when a
standard healthy diet was compared to a healthy diet enriched with walnuts, a healthy diet containing
walnuts was found to lower serum cholesterol levels more than the other diet at the end of the study.
Clinical and observational studies have demonstrated that the consumption of nuts reduces risk of
cardiovascular diseases. U.S. Food and Drug Administration (FDA) has indicated that there is a
significant effect of the consumption of nuts in reducing cardiovascular diseases risk. Moreover, the
American Heart Association emphasized that nuts must be found in a healthy dietary within the
context of 2020 health promotion and development. On the other hand, it is stated that excessive
consumption of nuts may lead to atherosclerosis due to high fat content, an increase in the body weight
and adipose tissue. In the last 20 years, with the increase in research on nuts, the importance of nuts
has become even more pronounced. By means of this situation, the macro and micronutrients
contained in the nuts are indispensable for nutrition. In addition, the relationship of nuts between
mortality, cardiovascular diseases, serum lipid levels, diabetes have been studied and they were found
to have positive effects on them in many studies. However, as with any nutrient, it must be consumed
in the amounts specified in the diet on nuts. Therefore, it is recommended that nuts are consumed as
much as the recommended daily amount 28 gr / day.

Corresponding Author: OSMAN BOZKURT, dytosmanbozkurt@gmail.com

1
GAZ NVERSTES

1034
KEYWORDS
Nuts, nutrition, body weight

1035
 Poster Session 7
Submission ID: 959

CUPRIC REDUCING ANTIOXIDANT CAPACITY (CUPRAC), DPPH

FREE AND ABTS CATION RADICAL SCAVENGING ACTIVITIES OF
ETHANOL EXTRACT FRACTIONS OF ENDEMIC SALVIA CERINO
PRUINOSA VAR. ELAZIGENSIS

1 1 2 1 1
EVIN AYGN TUNCAY , LEYLA BALUR , MEHMET FIRAT , SMAIL YENER , EYP TUNCAY , HILAL SARUHAN
3 1 4 2
FIDAN , ESRA YARI , MEHMET BOA , ABDULSELAM ERTA

ABSTRACT
The genus Salvia L. includes more than 900 species and is mostly found in both subtropical
and temperate parts of the world; the two largest gen centers of the Salvia are in America and
SouthWest Asia. In Turkey, endemism ratio of Salvia is 48 %, so Turkey is a major gen centre for the
Salvia genus. This genus is named Salvia, derived from latin Salveo, which means to save, to
recover. Salvia taxa is used in folk medicine from ancient times and find application in many
commercial and medicinal products, particularly in essential or volatile oils and flavoring agents
manufacture and is widely used in the food and cosmetic industries. 73 fractions were gained from
ethanol extract from the aerial parts of S. cerino pruinosa var. elazigensis. And also 37 fractions were
gained from the roots ethanol extract of this plant. According to the applied thin layer cromatography
results, totally 12 fractions from ethanol extract of aerial parts and 11 fractions from the root extract by
combining similar fractions. And also antioxidant activites were studied. In the DPPH free radical
scavenging activity it was determined that 41-44,45-48 numbered fractions from the extract of S.
cerino pruinosa var. elazigensis plants aerial part, showed higher activity than BHT and -TOC used
as standarts. And also 17-18, 21-26 fractions gained from root of S. cerino pruinosa var. elazigensis
extract, showed higher activity than BHT used as standart. In the ABTS cation radical scavenging
activity, it was determinated that the 41-44, 45-48, 49-56, 61-64, 65-69 ve 70-73 fragments which
-TOC
compounds used as standarts. When looked at the result of CUPRAC-Copper(II) reduction capacity,
aerial parts ethanol extracts 41-45 numbered fractions showed higher copper(II) reduction capacity
than the standarts was determined. According to the activitiy results generally the aerial parts activities
was higher than the root fractions activities was shown. Acknowledgements: The research was funded
by grant : KBAG 114Z801 from TUBITAK, The Scientific and Technological Research Council of
Turkey.

KEYWORDS

Corresponding Author: LEYLA BALUR, l_balur@hotmail.com

1
DCLE UNVERSTY, FACULTY OF PHARMACY, DEPARTMENT OF PHARMACOGNOSY, DYARBAKIR,
TURKEY
2
YZNC YIL UNVERSTY, FACULTY OF EDUCATON, DEPARTMENT OF BOLOGY, VAN, TURKEY
3
DCLE UNVERSTY, FACULTY OF PHARMACY, DEPARTMENT OF BOCHEMATRY, DYARBAKIR, TURKEY
4
DCLE UNVERSTY, FACULTY OF PHARMACY, DEPARTMENT OF PHARMACEUTCAL TECHNOLOGY,
DYARBAKIR, TURKEY

1036
Salvia cerino pruinosa var. elazigensis, Ethanol extract, DPPH, ABTS, CUPRAC

1037
 Poster Session 7
Submission ID: 960

DETERMINATION OF ANTIOXIDANT PROPERTIES OF INULA

HELENIUM ROOTS AND COTINUS COGGYGRIA LEAVES

1 1
NEVIM ALIKAN , NDER AYBASTIER

ABSTRACT
Inula helenium L. (Family: Asteraceae) is a widely occurring perennial herb in East Asia
particularly China, North American and Europe. The roots of I. helenium are used as versatile
medicine against fever, lung disorders, bronchitis, indigestion, chronic enterogastritis, and infectious
diseases. Cotinus coggygria Scop. (Family: Anacardiaceae) is a shrub or a tree usually growing up to 5
m. It grows mainly in South and Central Europe, South Russia, Crimea, Caucasia, Latakia and Turkey.
The leaves of this species have been used in Balkan and Anatolian folk medicine as antipyretic,
antiseptic, antihemorrhagic, treatment of diarrhoea and wound healing. The leaves and young branches
from naturally growing trees are utilized in producing an essential oil with terpenic odour. The
composition and antimicrobial activity of C. coggygria oils have been reported in different studies.
Antioxidants are substances that have the ability to delay, remove, or prevent the oxidation processes
occurring to other compounds. The measurement of the antioxidant capacity of plants has become very
important for researchers because it may provides information about resistance to oxidation,
quantitative contribution of natural antioxidant substances. Natural antioxidants are substances that
may protect human cells against the effects of produced free radicals. Free radicals can damage cells,
and may play a role in heart disease, cancer, and other diseases. Therefore, the antioxidant compounds
may play an important role in the prevention of certain diseases. The extracts of the plants were
obtained in methanolic, acidic methanolic and ethanolic, acidic ethanolic media using ultrasonic-
assisted extraction. The total phenolic contents of extracts were determined by Folin-Ciocalteu method
and also total antioxidant capacities of extracts were determined by ABTS and CHROMAC methods.
Phenolic compounds such as phenolic acids and flavonoids were analyzed in the extracts using high-
performance liquid chromatography-diode array detection (HPLC-DAD). The chlorogenic acid,
ferrulic acid in Inula helenium roots and myricetin, rutin, quercetin in Cotinus coggygria leaves were
determined. According to the results of these assays the extracts were showed to have good
antioxidant properties.

KEYWORDS
Inula helenium, Cotinus coggygria, antioxidant, phenolic compounds, HPLC

Corresponding Author: NDER AYBASTIER, aybastier@uludag.edu.tr

1
ULUDA NVERSTES

1038
 Poster Session 7
Submission ID: 961

BOTANIC ORIGIN AND ANTIOXIDANT ACTIVITIES OF THE

PROPOLIS FROM REFAHIYE (ERZINCAN) AS NUTRITIONAL
SUPPLEMENTS

1
LGIN KIZILPINAR TEMZER

ABSTRACT
Propolis is a resinous, sticky gum and used by bee as versatile material. In addition, biological
properties of propolis are potential for the improvement of new drugs and nutritional supplements.
Pollen analysis, antioxidant activities, total phenol and total flavonoid contents were studied at the first
time propolis sample from Refahiye (Erzincan). Mellisopalynological analysis was made according to
the relevant literature and botanic origin of sample, mainly from the Fabaceae (38.4%), Asteraceae
(20.2%) and Fagaceae (11.2%) families. The antioxidant capacity of propolis extract was assessed by
the hyrdogen peroxide scavenging activity (in terms of SC50), ferric reducing antioxidant power
capacity (FRAP), DPPH radical scavenging activity (in terms of SC50), metal-chelating activity (%),
total phenol content (TPC), and total flavonoid content (TFC). The values were found as 25.86 g/mL,
72.25%, 52.15 g/mL, 41.51%, 3163.85 mg GAE/100g and 118.59 mg CAE/100g, respectively. For
comparison of these results, Buthylated Hydroxy Anisole (BHA), Buthylated Hydroxy Toluene (BHT)
and -Tocopherol (TOC) were used as standard antioxidant compounds. The high activity of propolis
could be related with their different pollen composition. It could be beneficial for human health.

KEYWORDS
Antioxidant capacity, health, pollen, propolis, Refahiye(Erzincan)

Corresponding Author: LGN KIZILPINAR TEMZER, ilginc.kizilpinar@giresun.edu.tr

1
GRESUN NVERSTES

1039
 Poster Session 7
Submission ID: 962

PHENOLIC COMPOUNDS AND ANTIOXIDANT ACTIVITY OF

BUCKWHEAT POLLEN OF KONYA REGION

1 2 3 4
SEVGI KOLAYLI , OKTAY YILDIZ , ZEHRA CAN , MERYEM KARA

ABSTRACT
Pollens are male reproductive organs of flowering plants and are accepted as a food balanced
with proteins, sucrose, vitamins and minerals. Pollen is an essential nutrient for raising brood after
honeybee larvas and also for improving tissues, muscles, secretary glands and other organs sufficiently
during youth. Buckwheat is a species of wheat produced commonly in countries such as North
America, China, Europe, Poland, Holland and Russia and is not much well-known in Turkey.
Buckwheat, which started to be produced recently in our country in Konya plain as a pilot area, gives a
yield twice per year. Buckwheat, an important source of protein for the celiac patients, its honey and
pollen are also valuable products. In this study total phenolic content, total flavonoids, antioxidant
capacity (FRAP), and some phenolic compounds of the buckwheat pollens obtained from Konya
region were investigated. Phenolic compounds were determined by HPLC-UV. In a study performed
on three different pollens, the average total phenolic content, the average total flavonoid and total
antioxidant capacity were determined as 762 mg gallic acid /100 g fresh pollen, 75 mg quercetin/100 g
fresh pollen and 490 mikromol Trolox/100 g, respectively. It was found out that pollens were rich in
catechin, coumaric acid, ferulic acid and cinnamic acid.

KEYWORDS
Buckwheat pollen, phenolic compounds, antioxidant activity

Corresponding Author: MERYEM KARA, meryemkaram72@gmail.com

1
KARADENZ TEKNK NVERSTES, FEN FAKLTES, KMYA BLM
2
KARADENZ TEKNK NVERSTES, MAKA MESLEK YKSEKOKULU
3
GRESUN UNVERSTY, EBNKARAHSAR TECHNCAL SCENCES VOCATONAL HGH SCHOOL
4
SELUK UNVERSTY, UMRA VOCATONAL HGH SCHOOL, KONYA

1040
 Poster Session 7
Submission ID: 963

THE IMPORTANCE OF CARNATION (SZYGIUM AROMATICUM)

AND CASTOR OIL (RICINUS COMMUNIS L.)

1 1 1
SEVIM HAMZAEBI , FATMA ZTRK , HATICE GNDZ

ABSTRACT
Throughout history, people have used plants as the most important food ingredient. However,
healing plants have been evaluated for the treatment of diseases in all parts of the world and in all
cultures. In ancient cultures, the use of herbal plants and herbal medicines continues to be preserved
by being transmitted to them. Herbal treatments, which are among the alternative treatments and have
very effective results, are increasing in our country as well as in the whole world. All plants present in
the world are used for herbal medicines according to their contents and structures. Therapeutic features
in any disease have been discovered and are known to be herbal plants are proven. Carnation and
castor oil are also used by people for healing. Clove is one of the most used and useful plants with its
strong aroma and strong smell. Carnation oil obtained from carnation is extremely rich in terms of
phosphorus, sodium, potassium, calcium, hydrochloric acid, iron and vitamin C. It is good for
complaints such as indigestion, nausea, stinging, earache, cough, sputum, bronchitis, sinusitis, asthma,
colds in the treatment of infections of the throat and gums, wounds, cuts, mushrooms, rashes, insects
and infections. With the blooming of Indian flower seeds, Indian oil is obtained, which is a colorless,
slightly yellowish, clear and dark oil. It acts on small intestines to relieve constipation. Avoids hair
and eyelash pouring, especially in the hair curler is very useful. Antiseptic properties help fight
infections. It has excellent antioxidant properties that allow the body to fight harmful free radicals.
Anti-inflammatory properties help relieve inflammation in various parts of the body. Carnations and
castor oil, which are very important for human health, are also used as an anesthetic agent for fish in
the aquaculture sector worldwide. The use of these oils as an anesthetic increases the use of vegetable
sources.

KEYWORDS
Carnation oil, castor oil, herbal treatment, medicinal herbs, anesthetic

Corresponding Author: SEVM HAMZAEB, sevimhamzacebi@gmail.com

1
ZMR KATP ELEB NVERSTES SU RNLER FAKLTES

1041
 Poster Session 7
Submission ID: 964

POTENTIAL EFFECTS OF CAFFEINE CONSUMPTION ON ENERGY

BALANCE, WEIGHT CONTROL AND TYPE 2 DIABETES

1 1
OSMAN BOZKURT , NILFER ACAR TEK

ABSTRACT
Obesity is an important public health problem throughout the world and its prevalence is
30.3% in Turkey. There are two important ways to prevent obesity. These are to reduce energy intake
or to increase energy expenditure. The aim of this review is to examine the mechanisms of action of
caffeine on energy balance and the possible effects on obesity. The thermogenic effect of the
catecholamines from extracts of some plants is present. From catecholamines whose effects on energy
balance are indicated, caffeine; especially in tea and coffee varieties, capsaicin; red pepper and chili
pepper, ephedrine from ephedra is contained. Recently, it has been reported that approximately 80% of
the world's population consumes products containing caffeine. The effects of caffeine on the energy
mechanism are through the sympathetic nervous system. Even if the amount of caffeine taken in the
body is small, it stimulates the sympathetic nervous system. Fat oxidation and thermogenesis in the
control of the stimulated sympathetic nervous system are effective on the basal metabolic rate (BMR),
increasing energy expenditure for a certain period of time. Caffeine that may stimulate thermogenesis
and fat oxidation through inhibition of phosphodiesterase, an enzyme that degrades cyclic AMP and
through the antagonism of the negative modulatory effect of adenosine on the increased noradrenaline
release. Studies have also reported that caffeine increases thermogenesis, as well as the effect of
reducing nutrient intake. It was also observed that the intake of caffeine between 200-300 mg
increased the metabolic rate between 2% and 12%. It has been reported that regular consumption of
coffee has been studied in epidemiological studies and animal models that prevent weight gain and
increase glucose tolerance and is particularly effective in the prevention of type 2 diabetes. However,
the mechanisms that create these potentially beneficial effects are not fully explained. In a study
conducted in obese individuals, it was observed that the patients that were given caffeine and
ephedrine combination lost more body weight and fat than the ones just given placebo and only
ephedrine. On the other hand, it is known that excessive caffeine intake results in dehydration,
insomnia, hypercalciuria and hypertension. The European Food Safety Association (EFSA)
recommends up to 400 mg of daily caffeine intake and a single dose of 200 mg of caffeine do not raise
safety concerns for adults. As a result, it can be said that a moderate caffeine intake (daily <400 mg) is
beneficial in the prevention of obesity and in the maintenance of health, such as fat oxidation,
thermogenic effect and increased glucose tolerance in the body.

KEYWORDS
Caffeine, Energy expenditure, Thermogenesis

Corresponding Author: OSMAN BOZKURT, dytosmanbozkurt@gmail.com

1
GAZ NVERSTES

1042
 Poster Session 7
Submission ID: 965

IN VITRO CYTOTOXIC ACTIVITY OF SALVIA KURDICA FROM

ANATOLIA

1 2 2 3 2
HILAL SARUHAN FIDAN , LEYLA BALUR , ESRA YARI , SEVGI RTEGN , ABDULSELAM ERTA , MEHMET
4 5 6
FIRAT , UFUK KOLAK , GLATI TOPU

ABSTRACT
Salvia L. (Lamiaceae) species consists of about 900 species distributed throughout the world.
In Turkey, this genus is represented by 100 species, 53 (53%) of which are endemic. The genus is
named Salvia, derived from latin Salveo, means save, or recover. Many of Salvia species are
named adaay in Anatolia, Turkey, and used as herbal tea due to their antiseptic, stimulant, diuretic
and wound healing properties. Salvia species are generally known for their several pharmacological
effects including antibacterial, antituberculous, antiviral, cytotoxic and cardiovascular activities. In
this study, the cytotoxic activity of chloroform and ethanol extracts of Salvia kurdica was determined
by MTT method. In this study, human-derived cancer cell series and the Primary Dermal Fibroblasts
series were used. For this purpose, the breast cancer cell line (MCF-7), the colon cancer series (HT-29)
and the Primary Dermal Fibroblast Series (PDF) were provided. Preliminary work has been done to
optimize the number of cells to be placed in the plates for each cell series. 22,000 for MCF-7, 20,000
for HT-29, 12,000 cells for PDF were placed in a 96-well plate and the cells were allowed to sit for 24
hours at 37 C and 5% CO2 in a humid environment to adhere to the plate. After 24 hours, the cells
were treated with prepared extracts at 10 [mu] of different concentrations for 48 and 72 hours. After
48 and 72 hours of treatment, the cells were incubated with 10 [mu] l MTT solutions for 4 hours. After
incubation, a dark blue colored formazan was formed. After adding 100l solubilization buffer to the
cells and incubating overnight at 37 C in 5% CO 2 humidified atmosphere, the absorbance of
formazan at 570 and 690 nm was measured with a plate reader. Measurements at 690 nm were used as
reference absorbances. Ethanol and chloroform extracts of S. Kurdica showed cytotoxic effect only at
high concentrations against HT29 and MCF7 cell series was determinated. But it was determinated
that the extracts in these high consentrations showed cytotoxic effect on healthy fibroblast cell series.
Acknowledgements: The research was funded by grant : KBAG 114Z801 from TUBITAK, The
Scientific and Technological Research Council of Turkey.

KEYWORDS
Salvia kurdica, MTT, MCF7, HT29

Corresponding Author: LEYLA BALUR, l_balur@hotmail.com

1
DCLE UNVERSTY, FACULTY OF PHARMACY, DEPARTMENT OF BOCHEMSTRY, DYARBAKIR
2
DCLE UNVERSTY, FACULTY OF PHARMACY, DEPARTMENT OF PHARMACOGNOSY, DYARBAKIR
3
DEPARTMENT OF MEDCAL BOLOGY, FACULTY OF MEDCNE, DCLE UNVERSTY, DYARBAKIR
4
YZNC YIL UNVERSTY, FACULTY OF EDUCATON, DEPARTMENT OF BOLOGY, VAN
5
STANBUL UNVERSTY, FACULTY OF PHARMACY, DEPARTMENT OF ANALYTCAL CHEMSTRY,
STANBUL
6
DEPARTMENT OF PHARMACOGNOSY AND PHYTOCHEMSTRY, BEZMALEM VAKF UNVERSTY,
STANBUL, TURKEY

1043
 Poster Session 7
Submission ID: 967

THE POSSIBLE FUNGAL PATHOGENS LIMITING BASIL

PRODUCTION

1 2 1
GLSM PALACIOLU , GKSEL ZER , HARUN BAYRAKTAR

ABSTRACT
Basil (Ocimum basilicum L.), belonging to the family of Lamiaceae is an important medicinal
and aromatic plant. It is used as a raw material in the food and perfumery industry and in the control of
a wide range of plant disease with antimicrobial effect as well as used as a spice. There are many
significant plant pathogens that cause yield and quality losses in the basil growing areas in the world.
However, there is little information on the biology and control methods of these disease agents.
Fusarium wilt caused by Fusarium oxysporum f.sp. basilicum is the most destructive disease in the
basil growing areas worldwide. Also, gray mold (Botrytis cinerea), root rot (Rhizoctonia solani,
Pythium spp.), white rot (Sclerotinia sclerotiorum), mildew (Peronospora belbahrii), leaf spot
(Colletotrichum spp., Cercospora ocimicola) are common fungal pathogens, restricting basil
production. This study provided the detailed information on symptoms, morphology and disease
management of especially, Fusarium wilt and the other pathogens that may cause problems in basil
production of Turkey.

KEYWORDS
Basil, plant diseases, fungal pathogens, Fusarium wilt

Corresponding Author: GLSM PALACIOLU, gpalacioglu@ankara.edu.tr

1
DEPARTMENT OF PLANT PROTECTON, FACULTY OF AGRCULTURE, ANKARA UNVERSTY, ANKARA
2
DEPARTMENT OF PLANT PROTECTON, FACULTY OF AGRCULTURE AND NATURAL SCENCES, ABANT
ZZET BAYSAL UNVERSTY, BOLU

1044
 Poster Session 7
Submission ID: 968

ESSENTIAL OIL CONSTITUENTS OF THE LEAVES AND FRUIT OF

MALABAILA DASYANTHA (K. KOCH) GROSSH. FROM TURKEY: A
TRADITIONAL MEDICINAL HERB

1 2 2
KR HAYTA , GLDEN DOAN , EYP BACI

ABSTRACT
Members of Apiaceae usually possess a characteristic pungent or aromatic smell which is due
to the occurrence of essential oil or oleoresin in their different organs. Therefore, volatile oils of
Apiaceae plants have a wide application in aromatherapy. The essential oil composition of the leaves
and fruit of Malabaila dasyantha (K. Koch) Grossh. collected from natural habitats in Tunceli (Turkey)
were determined by hydrodistillation in 0.7% (weight/weight) yields. The essential oils were analysed
by gas chromatography and gas chromatography/mass spectrometry. A total of 25 compounds have
been identified constituting 96.7% in fruit and 97.8% in leaves of the essential oils of the taxon. The
predominant compounds were spathulenol (22.5%), palmitic acid (17.6%), stearic acid (10.5%) and
oleic acid (9.8%) in the oils from the fruit. The main constituents in leaves of the essential oils of the
taxon were found as germacrene D (34.8%), Iso spathulenol (11.3%) and bicyclogermacrene (9.8%).
Quantitative and qualitative variations were determined in the essential oil composition of different
parts of the studied plant. In the present study, the chemical essential oil composition of the leaves and
fruit of Malabaila dasyantha is determined and examined to indicate the source of particular essential
oils for flavour, fragrance and other potential usefulness.

KEYWORDS
essential oils, gas chromatography/mass spectrometry, Malabaila dasyantha

Corresponding Author: KR HAYTA, sukruhayta@hotmail.com

1
BTLS EREN NVERSTES
2
FIRAT NVERSTES

1045
 Poster Session 7
Submission ID: 969

ENDOPLASMIC RETICULUM STRESS IN PLANTS AND NEW

THERAPEUTIC APPROACHES TO PLANT DISEASES

1 2 3
SERKAN EN , MERVE EN , SEFA ELK

ABSTRACT
In eucaryotic cell, the Endoplasmic Reticulum (ER) is the major organelle in the synthesis of
proteins and in the modifications after synthesis. As a result of physiological events such as cell
differentiation or adaptation to certain environmental conditions, protein production capacity increases
in the ER organelle. If this condition becomes chronic, the balance between the rates of production and
folding of proteins in the ER organelle deteriorates and a so-called ER stress presentation emerges.
Since viruses that infect plant cells induce plant cells to produce hyperactive proteins in an abnormal
manner, consequences that lead to ER stress are encountered in such circumstances. For instance, it
was found that the transcription factor basic-region leucine zipper 60 (bZIP60) protein, which is one of
the ER stress markers in plants, was expressed when Potato Virus X (PVX) was infected with tobacco
(Nicotiana benthamiana) plant. Similarly, it was found that Bip3 (Heat Shock Protein 70-Hsp70) and
bZIP60 proteins as ER stress indicators were expressed together when the same plant was infected
with Garlic virus X (GarVX). The fact that the silencing of the genes of ER stress markers resulting
from the viral infections in plants using siRNA enables physiological changes that allow plants to
tolerate the viral attack by restricting the virus infection is included in the literature data. Since ER
stress plays a key role in virus replication and pathogenesis in plant cells in this regard, ER stress-
mediated signaling pathways in the development of broad-spectrum potent antiviral therapeutic agents
emerges as the target pathways for new generation antiviral treatments.

KEYWORDS
ER stress, Antiviral therapy

Corresponding Author: SERKAN EN, serkansen@aku.edu.tr

1
AFYON KOCATEPE NVERSTES, ATATURK SALIK HZMETLER MESLEK YKSEKOKULU
2
MULA SITKI KOMAN NVERSTES, FETHYE SALIK HZMETLER MESLEK YKSEKOKULU
3
AFYON KOCATEPE NVERSTES, TIP FAKLTES, TIBB BYOKMYA A.D.

1046
 Poster Session 7
Submission ID: 970

THE ANTIMICROBIAL EFFECT OF POMEGRANATE PEEL, APPLE

PEEL AND ARTICHOKE LEAF EXTRACT

1 1 1 1 1
FATMA ZTRK , HATICE GNDZ , SEVIM HAMZAEBI , GKNUR SRENGIL , ASENA NVER , M. DILCAN
1
AKPINAR

ABSTRACT
Introduction Synthetic antimicrobial agents are widely used to prevent the development of
pathogenic bacteria in foods, but concerns about the safety of these chemicals are increasing from day
to day. Because of the resistance of these microorganisms to these chemicals, fruits, vegetables,
spices, plants or their extracts have begun to be used in the preservation of raw or unprocessed foods,
pharmaceuticals, alternative medicine and natural therapies. Therapeutic use of plants is as old as
human history and there are many plant species that are used as traditional medicines. In this study, it
was aimed to determine the antibacterial effect of pomegranate peel extract, apple peel extract and
artichoke leaf extract against food pathogens by disk diffusion method. Material and method
Preparation of the extract The pomegranate peel, apple peel and artichoke leaves used in the study
were dried in drying oven at 50 oC. The dried samples were triturated in a blender. 20 gr sample was
weighed and placed in 100 ml of solvent (80% ethanol). It was kept in a shaking water bath for 6
hours at 50 oC. Then the filtration was done with filter paper. 100 ml (80%) of solvent were added on
it again and this process was repeated 4 times. After the final filtration phase, the ethanol in obtained
solution was evaporated using a rotary evaporator. Preparation of bacterial inoculums The stock
cultures of Listeria monocytogenes, Escherichia coli, Escherichia coli O157: H7, Bacillus cereus,
Salmonella enteritis and Staphylococcus aureus that are in liquid form were stored at -20 2 oC on
glycerol containing (20%; v/v). For the experiments, 100 L of stock cultures were transferred to 10
mL TSB medium, and the bacteria were activated by incubation at 30 oC for 24 hours. The activated
bacterial cultures were transferred to centrifuge tubes that each of them is 10 mL, and after centrifuged
at 5000 rpm for 10 minutes, bacteria inoculums to be used in experiments were prepared by adding
0.85% physiological saline on obtained pellet part and by being adjusted the cell densities according to
0.5 MacFarland (108 CFU / mL) haze. Disk diffusion method (Kirby-Bauer method) 24 hour cultures
of L. monocytogenes, E. coli, E. coli O157: H7, B. cereus, S. enteritis and S. aureus strains were made
into the standard density of 0.5 McFarland by diluting with sterile physiological saline. 0.1 ml of
bacteria from the bacterial inoculums prepared in this way was planted in petri dishes containing
MHA according to the spreading plate method. 25 l (0.25%, 0.50%, 1%, 1.5%, 2.5%, 5%, 7.5%, 10%
concentrations) of plant extracts were soaked into sterile empty discs placed in petri dishes in which
planting was made. The Petri dishes in which planting was made were left to the incubation process at
30 oC for 24 hours. After the incubation, the zone diameters composed were measured and thier
antibacterial activities were determined. Conclusion While, different concentrations of pomegranate
peel extract have antibacterial effect against L. monocytogenes, E. coli, E. coli O157: H7, B. cereus, S.
enteritis and S. aureus only 7.5% and % 10 concentrations of apple peel extract and artichoke leaf
extract have antibacterial effects on the B. cereus, E. coli, S. aureus. Antibacterial properties of apple

Corresponding Author: HATCE GNDZ, hatice.gunduz1@ikc.edu.tr

1
ZMR KATP ELEB NVERSTES

1047
peel and artichoke leaf extracts were found to be lower than pomegranate peel extract. It was also
found that the antibacterial activity increased as the extract concentration increased.

KEYWORDS
The Antimicrobial Effect of Pomegranate Peel, Apple Peel and Artichoke Leaf Extract

1048
 Poster Session 7
Submission ID: 971

MUSHROOMS OF NATURE

1 1 1
YK PEREN TRK , YAHYA ZDOAN , LALE SARYE AKAN

ABSTRACT
Natural mushrooms have been known with its nutritional and medical properties in many
cultures by people for many years. Different types of mushrooms are considered as functional foods
because of their effectiveness in preventing and treating many diseases. The aim of this research is to
examine the benefits of mushroom species that grow in the nature spontaneously for the community.
Edible mushrooms contain variable bioactive components with high level of protein, fiber, vitamins
and minerals. Mushrooms are used medically as well as consumed as food. The medical effects of
mushrooms have been stated as immunomodulatory, cardiovascular protective, detoxifying, antiviral,
antioxidant, antibacterial and antidiabetic. At the same time, natural mushrooms which are collected
by local people in Turkey, is a cultural interest for the individuals. Our country has a rich macrofungus
flora. It is expressed that mor, kanlca (ntar), tellice, ebe, kavak, kuzugbei, bolet, kt
(karnkara), dobalan, doru, kaykan etc. mushrooms are collected in Ankara at Kzlcahamam,
Elmada, ubuk, Ik Mountain, amldere Plateau regions by the public. For example the mushroom
named kuzugbei has a place in most important mushroom species both in our country and in the
world in terms of its nutritional and economic value. Collected natural mushrooms can be used for
commercial purposes and also it is a demand as food. In Turkish culinary culture, it is prepared and
consumed in various ways like roasting and drying. It is thought that exports of the natural edible
mushrooms may be increased further with the incentives and arrangements which can be made. For
contrubition to country economy and nutrition of the individuals, ways for identification and best
usage of edible mushroom species should be found.

KEYWORDS
mushroom, gathering, culture, nutrient, economy

Corresponding Author: YK PEREN TRK, oturk@ybu.edu.tr

1
ANKARA YILDIRIM BEYAZIT NVERSTES SALIK BLMLER FAKLTES BESLENME VE DYETETK
BLM

1049
 Poster Session 7
Submission ID: 973

COMPOSITION OF ESSENTIAL OIL TWO MEDICINAL PLANTS

(EUCALYPTUS CAMALDULENSIS DEHNH. AND VIBIRNUM
OPULUS L. )

1 1 2
GLDEN DOAN , EMEL AKBABA , MER KILI

ABSTRACT
Eucalyptus is one of the worlds important and most widely planted species belongs to the
family Myrtaceae. Several species of Eucalyptus are used in folk medicine as an antiseptic and against
infections of the upper respiratory tract, such as cold, influenza and sinus congestion. The essential oil
of Eucalyptus species showed a wide spectrum of antimicrobial, antifungal, anticandidal, antibacterial,
expectorant and cough stimulant activity. In this study, the chemical composition of the essential oil
from the leaves and fruits of Eucalyptus camaldulensis Dehnh. and aerial parts of Vibirnum opulus L.
grown in Mersin (Turkey) were analyzed by using GC and GC/MS techniques. The yields of essential
oils were 1.2% in leaf and 1.0% (v/w) in fruit of E. camaldulensis and identified representing 93.8%
and 99.0% of the total oils, respectively. The major constituents of leaves were p-cymene (42.1%),
eucalyptol (1,8- - -terpinol (10.7%), and in fruit were
eucalyptol (1,8 cineole) (34.5%), p- -terpinol (15.1%). Our results showed that
both oils has rich in terms of monoterpene hydrocarbons and oxygenated monoterpenes. Viburnum
opulus is from Adoxaceae family and it is reported that has anticancer, antiaging and antioxidant
activity. This species used for renal stone reduction. The yields of essential oil of V. opulus was 0.5%
- -
pinene (27.2%) and butanoic acid (15.8%) were the main compounds identified in the oil of Vibirnum
opulus.

KEYWORDS
Eucalyptus, Vibirnum, Essential Oil, Turkey

Corresponding Author: GLDEN DOAN, gdogan@firat.edu.tr

1
FIRAT NVERSTES FEN FAK. BYOLOJ BL./ELAZI
2
BNGL NVERSRES TEKNK BLMLER MYO/ PEYZAJ VE SS BTKLER PROGRAMI

1050
 Poster Session 7
Submission ID: 974

ESSENTIAL OIL COMPOSITION OF ANACYCLUS CLAVATUS

(DESF.) PERS. (ASTERACEAE) FROM TURKEY

1 2 3 2
M. YAVUZ PAKSOY , EYP BAGCI , SKR HAYTA , AZZE DEMRPOLAT

ABSTRACT
Anacyclus clavatus (Desf.) Pers is a medicinal plant, belonging to the Asteraceae family. This
plant used as food, in various treatments and applications. Anacyclus clavatus can be used in many
ethnopharmacological applications, equally against upset stomach and treat gastric ulser. The essential
oil of the aerial parts of this plant was obtained by hydrodistillation and analyzed by GC and GC-MS.
The essential oil yields of A. clavatus were determined as 0.3(v/w). Twenty six constituents were
identified and comprised 85.3 percentage of the total essential oil from A. clavatus. Palmitic (27.0 %),
linoleic acid (14.5%), 2-pentadecanone (6.6%) were determined as main compounds. The medicinal
importance of the plant and essential oil composition were discussed in the genera patterns.

KEYWORDS
Anacyclus clavatus, Essential oil, Palmitic acid, Linoleic acid

Corresponding Author: M. YAVUZ PAKSOY, mypaksoy@gmail.com

1
MUNZUR NVERSTES
2
FIRAT NVERSTES
3
BTLS EREN NVERSTES

1051
 Poster Session 7
Submission ID: 975

MACRO ALGAE: HEALTHY FROM SEA

1 1 1 1 1
SEVIM HAMZAEBI , M. DILCAN AKPINAR , ASENA NVER , FATMA ZTRK , HATICE GNDZ

ABSTRACT
Algae, called seaweed, is one of the important living resources of the sea. These aquatic
creatures of plant origin are evaluated in many industrial areas such as food, phycolloid, agriculture,
pharmacy, medicine and energy because of their rich contents. According to the size of the algae are
divided into two as micro algae and macro algae. Micro algae are in microscopic size and macro algae
are in sizes ranging from 1 - 2 cm to 40 - 50 m. Far East and South Asian countries, as well as
naturally collected, cultures are made, and seas are planted like black lands. Algae include bromine,
iodine, organic acids, monosaccharides, polysaccharides, agar, alginic acid, sterols, proteins and
vitamins. Algae was first used as color material in the cosmetics industry and in the Roman empire
during the time of Virjil and Heros. Macro algae is one of the foods that should be consumed on a
daily basis because it contains the nutrients needed by the human body. The use of macroalgae as an
additive, and even in medicine, is increasing. Compounds that are responsible for antibiotic activity
are commonly found in macroalgae. The most important of these are; Halogenated compounds,
alcohols, aldehydes, terpenoids, hydroquinones and Ketones. Minerals such as calcium, magnesium,
sodium and potassium are also preferred in thalassotherapy centers due to trace elements and vitamins
such as iron, copper, zinc and manganese. Macro-algae agar gels are also used in various products
such as perfumed underarm creams, sun creams and dermatological creams containing zinc oxide or
penicillin. It has also been noted that alginates used in creams have a feeling of freshness and
relaxation due to the rapid evaporation effect on the skin. In soaps and shaving foams, sodium alginate
is used as a lubricant, to impart an oily property to foam-free shaving creams, and to provide foaming
continuity in foams. In medicine and cosmetics industry, carrageenans are also frequently used
because of their fluid properties. They are used as stabilizers in shampoos, hair balms to protect foam
in shaving foams and soaps, and as absorption enhancers in products such as facial masks. Carrageenic
subdermal deodorants prevent the development of bacteria responsible for their relative degradation
and ultimately leading to unwanted sweat odors. In this study, the use of macroalgae in areas such as
health and cosmetics will be compiled.

KEYWORDS
Macro algae, algae, health, cosmetics, seaweed

Corresponding Author: SEVM HAMZAEB, sevimhamzacebi@gmail.com

1
ZMR KATP ELEB NVERSTES SU RNLER FAKLTES

1052
 Poster Session 7
Submission ID: 976

EFFECTS OF OREGANO (ORIGANUM SYRIACUM) ESSENTIAL OIL

ADDITION ON THE SHELF LIFE EXTENTION OF RAINBOW TROUT
(ONCORHYNCHUS MYKISS, WALBAUM, 1972) FILLETS

1 2 3
AYE ZYILMAZ , ABDULLAH KSZ , GLSN AKDEMR EVRENDLEK

ABSTRACT
Effects of oregano essential oil (Origanum syriacum) at the concentrations of 0 (control), 5, 20
-day of storage
were measured by the means of chemical, microbological and sensory analyses. At the beginning of

-N value of 18.9 mg
TVB-N/100 g reached to 39 mg TVB-N /100 g in control group (A), 33 mg TVBN-N /100 g in group
B (treatment with 5L/g), and 38.9 mg TVBN-N /100 g in group C (treatment with 20L/g ) and 43.1
mg TVBN-N /100 g in group D (treatment with 35L/g ) when fillets were rejected by panelists. The
spoilage of fillets was fastest both in control group and group B, while the spoilage of group C was
slower than group B during storage. Sensory evaluation was taken as the most important criteria for
the determination of shelf-life. The results have shown that oregano essential oil extended shelf-life of
rainbow trouts fillets. It was found out that the higher concentration of oregano essentialoil (35L/g)
provided the longer shelf-life of fillets up to 11 days at 20C compare to other treatments.

KEYWORDS
Fish fillet, essential oil, oregano (Origanum syriacum), rainbow trout (Onchorhynhus mykiss),
shelf-life

Corresponding Author: AYE ZYILMAZ, aylaayse@gmail.com

1
1ISKENDERUN TECHNCAL UNVERSTY, FACULTY OF MARNE SCENCE AND TECHNOLOGY,
SKENDERUN- HATAY
2
NECMETTN ERBAKAN NVERSTES
3
ABANT ZZET BAYSAL NVERSTES

1053
 Poster Session 7
Submission ID: 978

ESSENTIAL OIL COMPOSITION OF LALLEMANTIA PELTATA (L.)

FISCH. ET MEY. (LAMIACEAE) FROM TURKEY

1 2 3 2
SUKRU HAYTA , EYP BAGCI , M.YAVUZ PAKSOY , GULDEN DOGAN

ABSTRACT
The genus Lallemantia is represented by three taxons in the Flora of Turkey. Lallemantia
peltata (L.) Fisch. et Mey. is a widely used plant and has antifungal and antibacterial activities,
colloborating the traditional therapeutic uses, it can be used in the therapy of infectious diseases as
well as an antimicrobial additive in foods. The essential oil composition of Lallemantia peltata
obtained by hydrodistillation and analyzed by gas chromatography (GC) and gas chromatography -
mass spectrometry (GC-MS). The essential oil yields of L. peltata were determined as 0.2 (v/w) in
aerial part. 13 constituents were identified and comprised 94.7% of the total essential oil. Germacrene-
D (26.1 %), caryophyllene oxide (14.3%), 1H-Siklopro[e]azulen-7-ol (13.0%) and 2- pentadecanone
(9.1 %) were determined as main compounds of L. peltata. The results has shown that the parts of the
plant and essential oil may be used as source of natural product.

KEYWORDS
Lallemantia peltata, Essential oil, Germacrene-D, Caryophyllene oxide

Corresponding Author: SUKRU HAYTA, sukruhayta@hotmail.com

1
BTLS EREN NVERSTES
2
FIRAT NVERSTES
3
MUNZUR NVERSTES

1054
 Poster Session 7
Submission ID: 982

DATE PALM AND ITS ANTIOXIDANT EFFECT

1 1
SERKAN ASLAN , NEVIN ANLIER

ABSTRACT
Date palm has an important place in many ways in history of humanity. Several components
of date palm have positive effect on health. Date palm specifically involves components that have
antioxidant effects. This review aims to show date palms antioxidant components and conditions that
effect these components. Along with various phenolic compounds such as; p-coumaric, ferulic and
synaptic acids, date palm provides its antioxidant feature from carotene, flavanoid, procyanidin and
anthocyanin. Besides, date palm involves lutein, betacarotene, neoxanthin and as the largest
phytochemical, carotenoid. Date palm is rich in selenium which is an antioxidant mineral. However,
there are several apprehensions about date palms rich selenium amount (average 0.31 mg/100g) is
close to daily toxic level (0.85 mg). Date palms antioxidant content is influenced by many factors.
Dehumidification process affects date palms antioxidant components. During dehumidification,
carotenoid loss is detected when dried and fresh date palms carotenoid levels are compared. Again,
there are considerable amount of total polyphenol loss in dried date palm. Storage conditions are
affecting date palms antioxidant ingredient as well. Increase in total phenolics and flavonoids is being
occurred when date palm stored in +4C. Eventually, storage in refrigerator for a long time (until six
months) may be recommended in order to gain more antioxidant value from date palm. As a result
date palm is an important antioxidant source thanks to various antioxidant compounds that it is
containing. Consumption of 100 g of date palm (approximately 6-7 pieces) provides 80400 mol/100
g antioxidant and it compensates 50-100% of fiber and 11-15% of an adults daily energy requirement.
Contained antioxidant components may vary due to storage conditions and whether date palm is dried
or fresh.

KEYWORDS
date palm, antioxidant, nutrition

Corresponding Author: SERKAN ASLAN, serkanaslan43@gmail.com

1
GAZ NVERSTES

1055
 Poster Session 7
Submission ID: 983

NUTRITIONAL COMPOSITION AND HEALTH BENEFITS OF

EDIBLE MUSHROOMS

1 1 1 1
TUBA TATAR , NILFER ACAR TEK , GAMZE AKBULUT , MERVE PEHLVAN

ABSTRACT
Mushrooms and other fungi are special because of being neither plant nor animal. They have
been position in a place of their own, called Myceteae. In the world, there are more than 2000 species
of mushrooms. But less than 25 species are widely accepted as food. Mushrooms have a great
nutritional value since they are quite rich in protein, with an important content of essential amino acids
and fiber, and poor in fat. Edible mushrooms also provide a nutritionally significant content of
vitamins (B1, B2, B12, C, D and E). The crude protein content of edible mushrooms varies ranges
from 15% to 35% of dry weight. The protein quality of mushrooms is higher compared to most plant
proteins. The proteins of mushrooms are relatively rich in threonine, valine, glutamic acid, aspartic
acid, and arginine but are poor in methionine and cysteine. Mushrooms' carbohydrate content ranges
from 35% to 70% of dry weight. Mushrooms' carbohydrates include oligosaccharides such as trehalose
and cell wall polysaccharides such as chitin, -glucans and mannans. Because of these cell wall
components including non-digestible carbohydrates, mushrooms are also rich in dietary fiber. High
fiber intake is recommended for constipation. Fiber supplements containing ear mushrooms which
have the highest dietary fiber improves constipation related symptoms without serious side effects in
patients with functional constipation. The polysaccharides in mushrooms composition have
immunomodulatory characteristics such as the improvement of lymphocyte proliferation,
antitumorpromoting activities and antibody production. Mushrooms show antitumor activity due to
their -glucan content. -glucan content also demonstrates a hypoglycemic effect. They perform it via
a direct interaction with insulin receptors on target tissues. Mushrooms influence LDL and HDL
cholesterol, triacylglycerol, homocysteine and blood pressure that are generally accepted biomarkers
of cardiovascular diseases. When fat composition of mushrooms is examined, trans isomers of
unsaturated fatty acids which increase serum total cholesterol and high density lipoprotein ratio are not
detected. Dietary fibers of mushrooms also contribute this effect. In conclusion edible mushrooms
have beneficial effects on health with its rich nutrient and fibre composition. Also edible mushrooms
are called as functional foods because of their beneficial effects.

KEYWORDS
Mushrooms, Health Benefits, Nutritional Composition

Corresponding Author: TUBA TATAR, dyt.tugba@hotmail.com

1
GAZ NVERSTES

1056
 Poster Session 7
Submission ID: 985

THE USE OF STEVIA POWDER (STEVIA REBAUDIANA) AS A

SWEETING IN TURKSH DELGHT PRODUCTION

1
LYAS ELK , HSEYIN DOAN BOZKURT

ABSTRACT
One of the most fundamental objectives of the food industry is to guide efforts to meet
conscious consumer demands. Food industry workers are aiming at lowering the amount of sugar in
the diet (due to diabetes, obesity, chronic illnesses, etc.) in parallel with general tendency. In this
study, the sweetener for this problem was aimed at the production of stevia (Stevia rebaudiana) (sugar
plant, sugar candy) plant which is 250-300 times more sugar than normal sugar and which is
traditionally produced and used in the production of Turkish delight, which does not increase the level
of glucose . According to the results of the research, the sensory control of Turkish delight containing
0.03% stevia powder was found to differ from the Turkish delight in terms of taste and general taste. It
can be said that the obtained values are higher than the average, and that the use of stevia powder in
the production of Turkish delight is positive. When the color parameter was examined, samples of
stevia dusted with the lightest color were obtained. In terms of tackiness values; There was a
significant difference between control and Turkish delight samples in stevia Turkish delight samples
(p <0.05).

KEYWORDS
Sweetener, Stevia, Turkish Delight, Diabetes

Corresponding Author: LYAS ELK, ilyasc@pau.edu.tr

1
PAMUKKALE NVERSTES

1057
 Poster Session 7
Submission ID: 986

GREEN SYNTHESIS OF LAUROCERAUSUS OFFICINALIS ROEMER

(CHERRY LAUREL) FRUIT EXTRACTS BASED NANOFLOWER
WITH IN VITRO AMOEBICIDAL AND CATALYTIC ACTIVITY

1 2 1 1 1 2
AYE BALDEMIR , LK KARAMAN , SADI YUSUFBEYOLU , AYE EKEN , NILAY ILDIZ , CEMIL OLAK , GAMZE
2 1
AHIN , SMAIL SOY

ABSTRACT
The pathogenic Acanthamoeba often causes Acanthamoeba keratitis, which is an opportunistic
protozoan infection, related with soft contact lens wear. If not treated quickly, it is a type of infection
that can result in corneal ulceration, visual loss or even blindness. While there are many chemotherapy
options available in the treatment of Acanthamoeba infections, they are tough treatments and have
limited efficacy [1-3]. Hydrogen peroxide is a commonly used contact lens disinfectant and is
effective against Acanthamoeba. However, it is toxic to the cornea and must be rapidly neutralized
before the lens wears to avoid corneal damage. The single-stage hydrogen peroxide systems applied
for the rapid neutralization have very few cysticidal effects [4]. For these reasons, new, more
efficacious treatments are required for Acanthamoeba infections. Laurocerasus officinalis Roem.
(Cherry laurel) belongs to the Rosaceae family and is a popular fruit, commonly distributed in the
coasts of the Black Sea region of Turkey and is locally called "Taflan", "Laz kiraz" or "Karayemis".
Besides its use for food, in Turkey both fruit and seeds of cherry laurel are have been used for many
years for the treatment of stomach ulcers, digestive system complaints, bronchitis, skin diseases
(especially eczemas) and haemorrhoids [5]. In particular, there is information about the use of seeds
against parasites in this region. In previous studies it was determined that the fruits were rich in
phenolic components [6,7]. In present study, the effects on the proliferation Acanthamoeba castellani
of methanol extracts prepared from endocarp, mesocarp and seeds of the cherry laurel fruit and
nanoflowers (NFs) structures which is synthesized from these extracts were investigated [8]. Thus, for
the first time, novel organic-inorganic nanobio-antiparasitic agents called NFs were produced and the
increase in the amoebicidal activity of the NFs was elucidated. The NFs were characterized with
several techniques such as Scanning Electron Microscopy (SEM), Fourier Transform Infrared
Spectrometer (FT-IR), Energy-Dispersive X-ray (EDX) and X-ray Diffraction Analysis (XRD). In
addition, the catalytic activity of fruit extracts and the NFs were measured against guaiacol in the
presence of the H2O2. The viability test of Acanthamoeba castellanii cysts for amoebicidal activity
was performed using 4% trypan blue. Methanol extracts and NFs were prepared at concentrations of
32, 16, 8, 4, 2 and 1 mg / mL in 0.9% saline and distributed in 200 l of tubes. 200 l added to 98%
viable A. castellanii cysts (20X106 parasite/mL) were incubated at room temperature. In the statistical
analysis, the Kruskal-Wallis, Friedman and Conover tests were used to analyze significant differences
between the mean values. A p value of <0.05 was considered statistically significant. As a result, NFs
synthesized from fruit extracts were demonstrated about 5 times more effective than extracts alone for
Ameobicidal activity. This can be explained as an increase in the amobisidal activity of a new nano-

Corresponding Author: AYE BALDEMR, aysebaldemir@gmail.com

1
ERCYES NVERSTES
2
ORDU NVERSTES

1058
bio-antiparasitic agent known as nanoflower. References [1] Marciano-Cabral F, Cabral G (2003)
Acanthamoeba spp. as agents of disease in humans. Clin Microbiol Rev 16:273307 [2] Ertabaklar,
H., Dayanr, V., Apaydn, P., Ertu, S., & Walochnk, J. (2009). Olgu Sunumu: Acanthamoeba
Keratiti. Trkiye Parazitol Derg, 33, 283-285. [3] Tepe, B., Malatyali, E., Degerli, S., & Berk, S.
(2012). In vitro amoebicidal activities of Teucrium polium and T. chamaedrys on Acanthamoeba
castellanii trophozoites and cysts. Parasitology Research, 110(5), 1773-1778. [4] Hughes, R., Andrew,
P. W., & Kilvington, S. (2003). Enhanced killing of Acanthamoeba cysts with a plant peroxidase-
hydrogen peroxide-halide antimicrobial system. Applied and Environmental Microbiology, 69(5),
2563-2567. [5] Baytop T (1999). Therapy with medicinal plants in Turkey (past and present), 1st ed.;
Istanbul University: Istanbul, Turkey, pp. 178-249. [6] Karahalil, F. Y., & ahin, H. (2011). Phenolic
composition and antioxidant capacity of Cherry laurel (Laurocerasus officinalis Roem.) sampled from
Trabzon region, Turkey. African Journal of Biotechnology, 10(72), 16293-16299. [7] Kolayli, S.,
Kk, M., Duran, C., Candan, F., & Diner, B. (2003). Chemical and antioxidant properties of
Laurocerasus officinalis Roem.(cherry laurel) fruit grown in the Black Sea region. Journal of
agricultural and food chemistry, 51(25), 7489-7494. [8] Ge, J., Lei, J., Zare, R. N. Protein-inorganic
hybrid nanoflowers. Nature nanotechnology, 7(7), 428-432, 2012.

KEYWORDS
Laurocerasus officinalis; Acanthamoeba castellanii; Nanoflower; Catalytic activity;
Amoebicidal activity

1059
 Poster Session 7
Submission ID: 987

ANTIBACTERIAL ACTIVITIES OF CALENDULA OFFICINALIS

CALLUS EXTRACT

1 2 1
BURCU ETIN , FATIH KALYONCU , BETL KURTULU

ABSTRACT
The use of Calendula officinalis Linn. (Asteraceae) preparations for, topical application, is
widespread both in dermatology and in cosmetics and one of the relevant pharmacological activities
for this use is the anti-inflammatory one. It grows in the forests of India, China, Central Europe, and
some tropical areas. Plant tissue culture techniques enable the production of plant tissue or cells in
sterile environments under controlled conditions. By one of these methods, callus cultures,
pharmacologically active molecules could be produced at the desired amount and constant quality at
any time in laboratory conditions. This technique is an alternative method for obtaining the raw
material source required for the food, pharmaceutical and cosmetic industries. In this study, the
antimicrobial activity of C. officinalis callus derived from cotyledon explants were determined. The
seeds were immersed in 70% ethyl alcohol for 3 min, followed by surface sterilization with 0.5%
NaOCl solution for 5 min and rinsed with sterile double-distilled water. Later on, they were
germinated in jars containing 30 mL MS (Murashige and Skoog, 1962) medium without plant growth
regulators. Cotyledons excised from in vitro germinated seedlings were used as explants. They were
transferred on MS medium suplemented with benzil amino purine (BAP; 2 mg/l), -naphthalene-acetic
acid (NAA; 2 mg/l) for callus studies. The cultures were maintained on the same media compositions
and sub-cultured at an interval of four weeks. Callus cultures were harvested at the end of the 16th
week. Callus were dried at 40 C in the dark for antimicrobial studies. Calendula officinalis callus
extracts were tested for their antibacterial activities by using agar well diffusion method. Ethanol and
chloroform extracts from these callus were assayed against nine bacteria species (Staphylococcus
aureus ATCC 6538, Escherichia coli ATCC 25922, Bacillus cereus ATCC 7064, Bacillus subtilis
ATCC 6633, Salmonella typhimurium CCM 5445, Proteus vulgaris ATCC 6896, Enterococcus
faecalis ATCC 29212, Enterobacter cloacae ATCC 13047, and Kocuria rhizophila ATCC 9341). The
test antibiotics penicillin G, novobiocin, amphicillin, chloramphenicol and erythromycin were used for
comparison. Calendula officinalis seeds were germinated without any plant growth regulator on MS
medium in one week. Callus formation was observed at the end of the 5th week on cotyledon explants.
The extracts were prepared from 16-weeks-old callus. C. officinalis callus extracts showed 38 mm
inhibition zone against S. aureus, and chloroform extracts showed 32 mm inhibition zone against B.
cereus. These results are very close to the test antibiotics used and C. officinalis was found more
effective on gram positive bacteria.

KEYWORDS

Corresponding Author: BURCU ETN, burcu.cetin@dpu.edu.tr

1
DUMLUPINAR NVERSTES
2
CELAL BAYAR NVERSTES

1060
Calendula officinalis; Callus; Antibacterial activity; BAP, NAA

1061
 Poster Session 7
Submission ID: 991

SOME ECOLOGICAL AND PHYTOCHEMICAL PROPERTIES,

USAGE AREAS AND DISTRIBUTION OF JUNIPERUS COMMUNIS
VAR. SAXATILIS PALL. IN ERZURUM

1 2 3 4 3
MEHMET NAL , TURGAY DINDAROLU , ZHAL GVENALP , SEFA GZC , HAFIZE YUCA

ABSTRACT
Juniperus communis var. saxatilis Pall. is a species belonging to the Oxycedrus section in the
Juniperoideae subfamily of the Cupressaceae family. This species is 1-7 m high and is an evergreen
shrub in winter. It is grown in Bursa, Bolu, Kastamonu, Amasya, Sivas, Gmhane, Rize, zmir,
Kayseri, Tunceli, Bitlis and Denizli in Turkey. This study was carried out to determine some
ecological and phytochemical characteristics of this species with usage area and some data of
inventory in Erzurum province. In addition, the in vitro -glucosidase enzyme inhibition assay of
methanol extract of fruits of J. communis var. saxatilis plant was also evaluated in our study. Enzyme
inhibition activity of fruit extract was investigated using -glucosidase enzyme obtained from
Saccaharomyces cerevisiae and p-nitrophenyl--D-glucopyranoside as substrate. The results are
compared according to the Acorbose. This species has been identified in the study area between 2000-
2600 m elevations and spreads to about 4635 hectares. The species is mixed with pure or other tree
and shrub species on the north, north-east and north-west slopes. It is a tolerant plant. It grows in light
and semi-shaded environments. It grows well in coarse textured, well-aired and quickly warming soil.
It can grow up slightly acidic, neutral and slightly alkaline soil. Methanol extract showed enzyme
inhibition at 90% (IC50: 0.0525 mg / ml) at 1000 g / mL concentration; the standard substance
Acorbose showed enzyme inhibition of 26.10% (IC50: 4.5629 mg / ml) at the same concentration. In
previous phytochemical studies, flavonoids, polyprenols and monoterpenic hydrocarbons were
isolated from different parts of J. communis var. saxatilis This species has been reported to have
abortus potential as well as anti-infertility, antioxidant, antibacterial and anti-diabetic activities. It is
known that the fruits of the plant are effective in rheumatic diseases and gout diseases in addition to
the urine-boosting, sweating use among the people. Various organs used in medicine and cosmetic
industry is as skin diseases, worm-reducing, stimulant, antiseptic, sedative, antispasmodic. They also
provide a place for nutrition and welfare for wildlife. It is also a preferred plant species in landscape
arrangements.

KEYWORDS
Juniperus communis var. saxatilis Pall., Inventory, Soil, Ecology, -glucosidase inhibition

Corresponding Author: MEHMET NAL, mehmetonal@ogm.gov.tr

1
ERZURUM ORMAN BLGE MDRL
2
KAHRAMANMARA STMAM NVERSTES ORMAN FAKLTES ORMAN MHENDSL BLM
3
ATATRK NVERSTES ECZACILIK FAKLTES FARMAKOGNOZ BLM
4
ERZNCAN NVERSTES ECZACILIK FAKLTES FARMAKOGNOZ BLM

1062
 Poster Session 7
Submission ID: 992

GASTROPROTECTIVE EFFECTS OF ZIZIPHUS JUJUBA FRUIT

EXTRACT AGAINST ETHANOL-INDUCED GASTRIC MUCOSAL
HEMORHAGIC LESIONS IN RATS

1 2 2 3
MEHMET RAMAZAN BOZHYK , MUSTAFA CAN GLER , AYHAN TANYEL , ERDEM TOKTAY , ERSEN
2
ERASLAN

ABSTRACT
Purpose: Gastric ulcer is a common worldwide disease. The most common causes of gastric
ulcer are the consumption of alcohol, Helicobacter pylori, and the use of non-steroidal anti-
inflammatory drugs (NSAIDs). Excessive alcohol consumption usually increases the risk of gastric
mucosal damage. Thus, the experimental model of ethanol-induced gastric injury in rats is generally
used to investigate alcohol-induced gastric ulcer in humans and the main compounds involved in the
antiulcer activity. The underlying mechanisms of ethanol-induced gastric ulcer have not been fully
identified. In this study, it was aimed to investigate gastroprotective effects of extract of Ziziphus
jujuba fruit (Jujube), on ethanol-induced gastric ulcer by histopathological method. Method: In this
study, 32 Wistar albino female rats weighing 2574.3 gr were used and 4 groups were formed (n=8).
Nothing was applied to the control group. Purified water, 4ml/kg and 8ml/kg jujuba extract were
respectively administered intragastrically for 10 days in the groups treated with ethanol and jujuba
extract. On 11th day, 5 ml/kg ethanol was administered intragastrically to the rats and they were
sacrificed after 90 minutes. The gastric tissues were obtained. Macroscopic imaging was first
performed and then they were stored in 10% formalin until histological studies to be performed.
Findings: Histopathological evaluation was performed by comparing the mucosal images of the
control group. In evaluation of hematoxylin and eosin (H&E) staining, there was no degradation in the
gastric tissue in the control group. In the group with ulcer, it was observed that there were
degenerative changes in the surface epithelial cells and dilated gastric pit and gland structures and
neutrophil infiltration. In the group treated with 4 ml/kg jujuba extract, it was seen that there were the
partial irregularities in the gastric pits and the decrease in neutrophil infiltration. In the group treated
with 8 ml/kg jujuba extract, it was seen that the gastric pits were regular, the mucosa generally
appeared like that of the control group and there was the significant reduction in neutrophil infiltration
and the number of necrotic cells. In immunohistochemical evaluation of nuclear factor appa B (NF-
B) and caspase-3 immunopositivity, the group with ulcer had higher immunopositivity compared to
the control group. Moreover, it was seen that the groups treated with jujuba extract had lower
immunopositivity due to its dosage dependent effect compared to the group with ulcer. In order to
better understand the histopathological evaluation, histopathological damage was scored as - (none), +
(little damage), ++ (medium damage) and +++ (severe damage) according to the regularity of the

Corresponding Author: MEHMET RAMAZAN BOZHYK, mrbozhuyuk@gmail.com

1
DEPARTMENT OF HORTCULTURE, FACULTY OF AGRCULTURE, ATATURK UNVERSTY, 25240, ERZURUM
2
DEPARTMENT OF PHYSOLOGY, FACULTY OF MEDCNE, ATATURK UNVERSTY, 25240, ERZURUM
3
DEPARTMENT OF HSTOLOGY AND EMBRYOLOGY, FACULTY OF MEDCNE, ATATRK UNVERSTY,
25240, ERZURUM

1063
gastric mucosa, the depth of the mucosal injury, and the presence of hemorrhage and necrotic cells.
Result: Jujube extract showed gastroprotective effects on ethanol-induced gastric damage in a dose-
dependent manner.

KEYWORDS
Jujube (Ziziphus jujuba), Gastroprotective effect, Ethanol-induced gastric ulcer

1064
 Poster Session 7
Submission ID: 993

A COMPARATIVE STUDY ON ESSENTIAL OIL COMPONENTS OF

CULTIVATED (CORIANDRUM SATIVUM L.) AND WILD
(CORIANDRUM TORDYLIUM (FENZL)) CORIANDER SPECIES

1 1 1 1 1
TAHSIN KARADOAN , ARIF ARIF ANLI , HASAN ZELK , BEKIR TOSUN , MERVE KURUNATAN

ABSTRACT
Essential oil content and composition of cultivated (Coriandrum sativum L.) and wild
(Coriandrum tordylium (Fenzl)) coriander species were compared in this study. The essential oils of
the herb and fruits extracted by hydro-distillation in a clevenger apparatus and the essential oils
analyzed by gas chromatography mass spectroscopy (GC-MS). Essential oil yield of C. sativum and C.
tordylium were 0.36% and 0.21% in the herbs and 0.65% and 0.35% in the fruits respectively, based
on v/w. The herb oils of C. sativum and C. tordylium contained 32 and 51 compounds where the major
compounds of C. sativum are cis-dec-2-enal (21.33%), anethole (14.88%), linalool (14.36%) and n-
decanal (12.04%) and C. tordylium were cis-dec-2-enal (21.33%), epoxylinalol (12.32%), capric acid
(8.72%) and 2-decenoic acid (7.78%). A total of 42 and 29 components were identified in the fruit oils
of C. sativum and C. tordylium, respectively. Main constituents of the fruit oils were respectively
linalool (56.29%) (69.25%), -pinene (9.96%) (5.92%), geranyl acetate (9.62%) (4.81%) and -
terpinene (2.19%) (7.61%). Essential oil compositions of both species varied qualitatively and
quantitatively. Acknowledgements This research was financially supported by TUBTAK (Trkiye
Bilimsel ve Teknolojik Aratrma Kurumu, grant number: 113O284)

KEYWORDS
Coriandrum sativum L., Coriandrum tordylium (Fenzl), essential oil composition

Corresponding Author: ARF ARF ANLI, arifsanli@sdu.edu.tr

1
SLEYMAN DEMREL NVERSTES

1065
 Poster Session 7
Submission ID: 995

CARDIOPROTECTIVE EFFECTS OF THE QUERCETIN AND RUTIN

IN 5-FLUOROURACIL-INDUCED CARDIOTOXICITY IN THE RATS

1 2 1
EMIN ENGL , VOLKAN GELEN , SEMIN GEDIKLI

ABSTRACT
Introduction and aim: 5-Floruracil (5-FU) is widely used in the solid tumors such as lung,
breast and gastrointestinal cancers. 5-FU causes cardiotoxicity and hepatotoxicity. In this study, in the
5-FU-induced cardiotoxicity has aimed investigations of the cardioprotective effects of Quercetin (Q)
and Rutin (RU) Method: In this study, 50 piece male Sprague Dawley rats (25025 g) were used. Rats
were divided randomly into eight group, which there was 10 rats in each group. The group control was
given intragastric (ig) corn oil (1 ml) for 14 days. The group 5-FU rats were given ig corn oil for 14
days and eleventh day injected intraperitoneal (ip) a single dose (50 mg/kg) of 5-FU. Group Q50+5-
FU and Q100+5-FU were given ig 50 mg/kg and 100 mg/kg Q for 14 days, respectively. These groups
were injected 5-FU (50 mg/kg) single dose on the 11th days of Q application. The group Q100 was
given Q (100 mg/kg-i.g) for 14 days. Group RU50+5-FU and RU100+5-FU were given ig 50 mg/kg
and 100 mg/kg doses of the RU for 14 days, respectively. Also, these groups were injected the single
dose of 5-FU (50 mg/kg) in the 11th days of RU application. The group RU100 was given RU (100
mg/kg-i.g) for 14 days. In the end experimental applications, the blood sample were collected from
anesthetized rats and rats were scarified. Sera were separated by centrifugation and utilized for the
evaluation of various cardiac marker enzymes (CK, CK-MB, AST, ALT, LDH, cTnI). The cardiac
tissues used for biochemical and histopathological analysis. The data were analyzed by Tukey test in
the one-way ANOVA. Results: When data are showed compared among groups that in the MDA level
was significantly higher in the 5-FU group than control group and decreased significantly RU100+5-
FU and Q100+5-FU groups. SOD and GSH levels were markedly decreased in the 5-FU group
compared with control, RU100+5-FU and Q100+5-FU groups. AST, CK, CK-MB, ALT, Troponin I
and LDH levels were significantly increased in the 5-FU group when compared to other groups. In the
histopathological examination of cardiac tissue was determined that in the 5-FU group had markedly
degenerated cells and cardiac myofibril. Intensity of -MHC positivity was higher in the 5-FU group
sections compared to the control, RU100+5-FU and Q100+5-FU groups sections. Conclusion: In the
present study was determined that especially high doses of the Q and RU have protective effects on 5-
FU-induced cardiotoxicity.

KEYWORDS
5-FU, Cardiotoxicity, Quercetin, Rutin, Rat

Corresponding Author: EMN ENGL, emin.sengul@atauni.edu.tr

1
ATATRK NVERSTES VETERNER FAKLTES
2
KAFKAS NVERSTES VETERNER FAKLTES

1066
 Poster Session 7
Submission ID: 996

THE EFFECT OF STORAGE ON SOME PROPERTIES OF 3

DIFFERENT GROUND POPPY SEED FATS

1 1
BESIM MADEN , SEDA YALIN

ABSTRACT
The poppy seed is a traditional product in Afyon. There are there type poppy seed. These are
white poppy seed, yellow poppy seed and gray poppy seed. The objective of this study is to investigate
the effect of storage at 15-20 C for several times (0, 7, 15, 30, 45 and 60 days) on some properties of
3 different poppy seed fats. In this study, fats were obtained from white poppy seed (TMO1), yellow
poppy seed (TMO2) and gray poppy seed (Afyon-95). Then free fatty acid amount (%), peroxide value
(meq O2/g) and iodide number of these fats were analyzed. Free fatty acid amount, peroxide value and
iodide number at these fats were increased with increasing of storage time, but this increase was little.
The results of 3 different poppy seed fat were found as similar. It was concluded that three different
poppy seed fats were stabile for long times at 15-20 C. It was investigated the effects of storage on
L*, a*, b* color values of three types of poppy seed fats and total phenolic contents of these fats were
determined. The fat contents of three types of poppy seeds were found as 53.85 % (white poppy seed),
49.85% (yellow poppy seed) and 49.17 % (gray poppy seed). L* and b* color values of white poppy
fat and gray poppy seed fat were decreased when storage time was increased, while a* color values of
those were increased. L*and a* color values of yellow poppy seed fats were increased when storage
time was increased, whereas b* color value of that was decreased.

KEYWORDS
fat, phenolic, poppy seed, storage, stability

Corresponding Author: BESM MADEN, b.maden@hotmail.com

1
AFYON MESLEK YKSEKOKULU, GIDA TEKNOLOJS PROGRAMI, AFYON KOCATEPE NVERSTES,
AFYONKARAHSAR, TRKYE

1067
 Poster Session 7
Submission ID: 999

EFFECT OF ACCELERATED SOLVENT EXTRACTION (ASE)

SYSTEM TEMPERATURE ON THE AMOUNT OF PHENOLIC ACIDS
IN THE METHANOLIC EXTRACT OF CHAMOMILE (MATRICARIA
CHAMOMILLA L.)

1 1 1 1
TIMUR TONGUR , SERPIL KILI , TANER ERKAYMAZ , MURAT KILI

ABSTRACT
Chamomile (Matricaria chamomilla L.) is one of the prefered hot beverage as herbal tea. The
aim of this study was to determine effective methanolic extraction temperature of chamomile
(Matricaria chamomilla L.) for phenolic acids by using accelarated solvent extraction (ASE). In the
study a commercial chamomile tea was used as a testing material. Detection of phenolic acids were
done with LC-ESI-MS/MS. The study was performed at 5 different temperatures; 40C, 50C, 60C,
80C and 100C. 1.0 g chamomile tea sample and 1.5 g diatomaceous earth weighed to the 10 ml ASE
extraction cell. Extraction time was 25 minutes for one sample. Nitrojen flow was used for drying
extract. Hydrolysis step were implemented to dried samples. Phenolic content of hydrolysed
chamomile samples were determined with LC-ESI-MS/MS. Caffeic acid, p-coumaric acid, rosmarinic
acid, ferulic acid, quercetin, chlorogenic acid, isorhamnetin, luteolin, syringic acid, and apigenin were
analysed in the study. For chromatographic separation Hypersil Gold 50 mm x 2.1 mm x 1.9m C18
column was used with gradient flow. Analysis time was for 8 minutes for one sample. LC-MS/MS
method was found to be selective, linear (r2>0.99) and precise for all of interested phenolic
compounds. The results showed that phenolic compounds were sensitive to temperature. The
methanolic extract which obtained at 40C had a highest phenolic acid values comparing to other
temperatures. The extraction yield was better at 40C for methanolic ASE extract.

KEYWORDS
Chamomile, Phenolic Compounds, Accelerated Solvent Extraction, LC-MS/MS

Corresponding Author: TMUR TONGUR, timurtongur@akdeniz.edu.tr

1
AKDENZ NV. GIDA GVENL VE TARIMSAL ARATIRMALAR MERKEZ

1068
 Poster Session 7
Submission ID: 1002

GASTROPROTECTIVE EFFECTS OF PERSIMMON (DIOSPYROS

KAKI L.) AGAINST ETHANOL-INDUCED GASTRIC ULCER IN RATS

1 2 1 1
MUSTAFA CAN GLER , MEHMET RAMAZAN BOZHYK , AYHAN TANYEL , ERSEN ERASLAN , ERDEM
3
TOKTAY

ABSTRACT
Aim: Gastric ulcers are a major problem worldwide with no effective treatment. Ulcer is
caused by an imbalance between two factors: aggressive factors in the lumen (physical, chemical or
psychological) and protective mechanisms. Aggressive factors include acid, pepsin, Helicobacter
pylori, stress, alcohol, and use of non-steroidal anti-inflammatory drugs (NSAIDs). Protective
mechanisms include mucus, bicarbonate, prostaglandin, blood flow, antioxidant system, nitric oxide,
and cell proliferation. Current treatments are not always effective. They also have side effects and are
expensive. Therefore, the protective effect of herbal medicines is important. Persimmon (Diospyros
kaki L.) is widely used in the treatment of various diseases. Previous studies have shown that
Diospyros kaki L. has anti-oxidant activity. Our study aimed to investigate gastroprotective effects of
persimmon (Diospyros kaki L.) on ethanol-induced gastric ulcer by histopathological method.
Method: In our study, 32 Wistar albino male rats weighing between 250-300 gr were used. We formed
4 groups each containing 8 rats. Group 1 was the control group and was placed on a normal diet.
Group 2 was the ethanol group and purified water was administered by oral gavage for 10 days. Low
dose (4 ml/kg) and high dose (8 ml/kg) persimmon extract were administered by oral gavage for 10
days in Group 3 and 4, respectively. In our study, we used Japanese persimmon (Diospyros kaki L.)
harvested in the ninth month in Mersin Province, Turkey. At the end of the 10th day, ethanol (absolute
ethanol 99%, 5mL/kg) was administered by oral gavage in order to produce gastric ulcer in Group 2, 3
and 4. The animals were sacrificed at the end of the experimental period (90 min). The gastric tissues
obtained from animals were investigated by histopathological methods. Results: Hematoxylin-eosin
staining results; Histopathological evaluation was performed based on the gastric mucosa.
Accordingly; In the control group, it was seen that the gastric pits were regular and the mucosal cells
were in normal size and shape. In the group with ulcer, it was seen that the gastric pits lost their
normal appearance. However, it was remarkable that the mucosal cells were necrotized in both the
superficial and deep layers of the mucosa and there was an increase in the number of lymphocytes. In
the group treated with low dose (4 ml/kg) persimmon extract, it was seen that the gastric pits were
regular and the surface mucosal cells had hypertrophic changes. In the group treated with high dose (8
ml/kg) persimmon extract, it was seen that the gastric pits were regular and the mucosa was generally
similar to that of the control group. In order to better understand the histopathological evaluation,
histopathological damage was scored as - (none), + (little damage), ++ (medium damage) and +++
(severe damage) according to the regularity of the gastric mucosa, the depth of the mucosal injury, and

Corresponding Author: MUSTAFA CAN GLER, mcanguler@yahoo.com

1
DEPARTMENT OF PHYSOLOGY, FACULTY OF MEDCNE, ATATRK UNVERSTY, 25240, ERZURUM
2
DEPARTMENT OF HORTCULTURE, FACULTY OF AGRCULTURE, ATATRK UNVERSTY, 25240, ERZURUM
3
DEPARTMENT OF HSTOLOGY AND EMBRYOLOGY, FACULTY OF MEDCNE, ATATRK UNVERSTY,
25240, ERZURUM

1069
the presence of hemorrhage and necrotic cells. The results of immunohistochemical evaluation In
order to better understand the results of immunohistochemical evaluation, NF-B and caspase-3
immunopositivity was scored as - (none), + (little damage), ++ (medium damage) and +++ (severe
damage). In immunohistochemical staining performed with NF-B and Caspase-3 antibody, while the
group with ulcer showed severe immunopositivity, the control group, the group treated with low dose
persimmon extract and the group treated with high dose persimmon extract showed mild
immunopositivity. Conclusions: It has been shown that persimmon (Diospyros kaki L.) may have
protective roles against ethanol-induced gastric ulcer. Persimmon (Diospyros kaki L.) can be
considered as a new potential natural method in gastric ulcer treatment. This study was supported by
Atatrk University SRP (Project no: 2016/051).

KEYWORDS
Gastric ulcer, Diospyros kaki L., Gastroprotective effect, Histopathological evaluation

1070
 Poster Session 7
Submission ID: 1003

GASTROPROTECTIVE EFFECTS OF PYRUS COMMUNIS AGAINST

ETHANOL-INDUCED GASTRIC MUCOSAL HEMORRHAGIC
LESIONS IN RATS

1 1 2 1
ERSEN ERASLAN , AYHAN TANYEL , MEHMET RAMAZAN BOZHYK , MUSTAFA CAN GLER , ERDEM
3
TOKTAY

ABSTRACT
Aim: Gastric ulcer is one of the most common diseases in the world and is a global problem.
Excessive alcohol consumption usually increases the risk of gastric mucosal damage. Herbal
medicines are used in the prevention of many diseases. In this study, an extract of pear fruit which
belongs to the Rosaceae family was used. Pear extract contains a large number of flavonoids, acids
and antioxidants. In this study, gastroprotective effects of pear extract on ethanol-induced gastric ulcer
in rats were investigated by histopathological methods. Method: In the study, 4 groups were created by
using 32 male wistar albino rats (n=8). Nothing was applied to the control group. Purified water,
4ml/kg and 8ml/kg pear extract were respectively administered intragastrically for 10 days in the
groups with ulcer and treated with pear extract. On 11th day, 5 ml/kg ethanol was administered
intragastrically to the rats and they were sacrificed after 90 minutes. The gastric tissues were obtained.
Macroscopic imaging was first performed and then they were stored in 10% formalin until histological
studies to be performed. Results: Histopathological evaluation was performed based on the gastric
mucosa. In evaluation of hematoxylin and eosin (H&E) staining, there was no degradation in the
gastric tissue in the control group. It was clearly seen that gastric pits lost their normal appearance in
the group with ulcer. However, it was remarkable that mucosal cells were necrotized in both the
superficial and deep layers of the mucosa and there was an increase in the number of lymphocytes. In
the group treated with 4 ml/kg pear extract, it was seen that there were the partial irregularities in the
gastric pits and the necrotic cells on the mucosal surface. In the group treated with 8 ml/kg pear
extract, it was seen that the gastric pits were regular, the mucosa generally appeared like that of the
control group and the necrotic cells were rarely found on the mucosal surface. In
immunohistochemical evaluation of nuclear factor appa B (NF-B) and caspase-3 immunopositivity,
the group with ulcer had higher immunopositivity compared to the control group. Moreover, it was
seen that the groups treated with pear extract had lower immunopositivity due to its dosage dependent
effect compared to the group with ulcer. In order to better understand the histopathological evaluation,
histopathological damage was scored as - (none), + (little damage), ++ (medium damage) and +++
(severe damage) according to the regularity of the gastric mucosa, the depth of the mucosal injury, and
the presence of hemorrhage and necrotic cells. Conclusion: Pear extract showed gastroprotective
effects on ethanol-induced gastric damage in a dose-dependent manner. This study was supported by
Atatrk University SRP (Project no: 2016/051).

Corresponding Author: AYHAN TANYEL, ayhan.tanyeli@atauni.edu.tr

1
DEPARTMENT OF PHYSOLOGY, FACULTY OF MEDCNE, ATATURK UNVERSTY, 25240, ERZURUM
2
DEPARTMENT OF HORTCULTURE, FACULTY OF AGRCULTURE, ATATURK UNVERSTY, 25240, ERZURUM
3
DEPARTMENT OF HSTOLOGY AND EMBRYOLOGY, FACULTY OF MEDCNE, ATATRK UNVERSTY,
25240, ERZURUM

1071
KEYWORDS
Gastric ulcer, Pyrus communis, Gastroprotective effect, Histopathological evaluation

1072
 Poster Session 7
Submission ID: 1004

MEDICAL BENEFITS OF BLACK CUMIN (NIGELLA SATIVA)

PLANT

1
GLTEN ENOCAK SORAN

ABSTRACT
Nigella sativa Ranunculaceae family of flowers, 20-30 cm high, with a bluish-green plant. For
this plant in our country names such as blackcurrant, black cumin and black cumin seeds are used. It
grows in western and central parts of the country (Afyon, Burdur, Isparta) while our country is grown
in countries like Southern Europe, Russia, Sudan, Ethiopia, Syria, Iran, Afghanistan and India. The
homeland is the Eastern Mediterranean countries, Eastern and Southern Europe. The seeds of the plant
are black and cornered, carrying essential oils. The seeds of this plant are used in the food industry due
to its aromatic nature, in some decorations (biscuits, muffins, etc.) and as a flavor in foods such as
overalls and sediment. They are also used as medicines for the treatment of various diseases. N. sativa
seeds are used in many countries for bronchial asthma, rheumatism, allergic diseases, various digestive
disorders and parasitic infections, and it is reported that there is no side effect when used correctly and
in the right dose. N. sativa seeds increase interleukin-3 (IL-3) secretion from T lymphocytes. It has
also been reported that Pseudomonas aeruginosa, Escherichia coli, Bacillus subtilis, Streptococcus
faecalis and antibacterial activity against various bacterials causing dental caries. It can also be used as
a preservative in the storage of certain foods by taking advantage of this antibacterial property of N.
sativa. N. sativa has been reported to contain over 100 substances in its chemical content, 38% of these
substances are reported to be carbohydrates (glucose, xylose, arabinose), 0.38-0.49% essential oil, 30-
40% fixed fat, 20-30% protein, saponin, melanin, nigellin and tannin. rekotu is used in the
treatment of many diseases in Far East and Middle East countries over 2000 years. It has been reported
that N. sativa proteins have antioxidant effect and regulate immunological response.

KEYWORDS
Crekotu, Nigella sativa, medical plant

Corresponding Author: GLTEN ENOCAK SORAN, gsenocak63@hotmail.com

1
HARRAN NVERSTES

1073
 Poster Session 7
Submission ID: 1397

PHYTOCHEMICAL STUDIES OF NIGELLA SATIVA IN SYRIAN AND

BURDUR SPECIMENS

1 1 1 2
SENA AKDOAN , BRAHM DEMRTA , FATH GL , SA TELC

ABSTRACT
rek otu are the Latin name Nigella sativa which is known to be 12 species in the family of
wedding flowers (Ranunculaceae) and especially in Turkey. It is also grown in most parts of the
world. The leaves that grow in Turkey such as Isparta, Konya, Burdur are fine and have a small seed
of black seeds about 35-40 cm in length. Syrian and Burdur plants seeds were initially stored at 1:1 in
MeOH and CH3Cl solvent mixtures and extractions were carried out. The same plants seeds
specimens were then crushed in the air and kept in the same solvent mixture in the same manner and
the extraction procedure was repeated for four times. Nigella sativa seeds extracts obtained from
Burdur and Syrian were primarily esterified and then fatty acids analysis was performed using GC-MS
method. In the obtained results, the main component was identified as linoleic acid in both samples.
The second and third major components were identified as oleic and palmitic acids. These three acids
were found to be more than 90% of total fatty acids. Also, in this study, isolation and esterification
techniques of fatty acids will be discussed. In particular, the effects of saturated and unsaturated fatty
acids on activity will be presented. Analyzes and effects of essential oils and especially anti-cancer
properties will be presented. In addition to this, the amount of the volatile contents will be also
presented.

KEYWORDS
Nigella sativa, essential oil, fatty acids

Corresponding Author: SENA AKDOAN, sena___akdogan@hotmail.com

1
ANKIRI KARATEKN NVERSTES
2
SLEYMAN DEMREL NVERSTES

1074
 Poster Session 8
Submission ID: 1006

INVESTGATON OF ANTMCROBAL EFFECT OF SUMAC AND

CNNAMON EXTRACTS ON PARSLEY

1 1 1
OKTAY TOMAR , GKHAN AKARCA , TESLIME EKZ

ABSTRACT
It is very important to consume healthy foods during healthy growth and development. It is
possible for people to grow up and to continue their lives by consuming healthy ingredients, starting
from raw materials. Foodborne microorganisms, which contaminate the food and food raw materials at
various stages, can lead to food poisoning and infections. Today, various processes (low temperature
or heat treatment application, packaging methods, etc.) and additives such as salt, sugar and
antimicrobial additives are used in order to prolong the shelf life and preservation of food. Consumers'
skepticism towards these additives has increased because some of the additives used are unhealthy and
can be carcinogenic and toxic depending on the usage rate. So, the acquisition and use of natural and
reliable additives has become very important. Nowadays, there is increasing interest in the using of
various plant extracts as antimicrobial agents to prevent microbial degradation and prolong shelf life.
In this study antimicrobial properties of Rhus L. (sumac) and Cinnamomum L. (cinnamon) were
investigated. Parsleys, obtained from the district bazaar, was washed with sumac and cinnamon
extracts prepared in different concentrations In the study, 6 applications (control (unwashed), 1%,
2.5% and 5% sumac and cinnamon extracts) were performed. Total aerobic mesophilic bacteria,
coliform group bacteria, coagulase positive Staphylococcus bacteria, Lactobacillus spp. bacteria, yeast
and mold counts, Listeria bacterium and Salmonella spp. were investigated in the obtained samples.

KEYWORDS
Rhus L., Cinnamomum L., parsley, extract, antimicrobial

Corresponding Author: OKTAY TOMAR, oktomar@aku.edu.tr

1
AFYON KOCATEPE NVERSTES MHENDSLK FAKLTES GIDA MHENDSL BLM

1075
 Poster Session 8
Submission ID: 1007

EVALUATION OF SPICE USE, STYLES AND FREQUENCY OF

ADULTS

1 1 1 1
DUYGU AAGNDZ , NILFER ACAR TEK , GAMZE AKBULUT , MERVE PEHLIVAN

ABSTRACT
Aim: This research was conducted to evaluate the spice use, styles and frequency of adults
aging between 18 and 64. Material and Method: The research was conducted on total 1125 (244 males
781 females) adults aged between 18 and 64. Participants were asked to fill out a questionnaire form
by means of face to face interview in order to determine their general characteristics of the participants
and their spice use, consumption styles (adding to dishes, making tea or consuming in powder form)
and frequency of consumption (every day, once a week, twice a week, three times a week). Results:
91.1% of the participants indicated that they use red pepper and add the spice to their dishes (98.9%)
mostly. 54,7 of the participants use red pepper (1.370.74 tea spoon) every day and 20.5% of them
three times a week. 43.8% of the participants use black pepper (1.160.61 tea spoon) every day,
24.0% of them three times a week and 14.8% of them twice a week. 99,1% of the participants using
black pepper (85,8%) stated that they add the spice to their dishes. 97.0% of the participants using isot
(Urfa pepper, 29.7%) add the spice to their dishes and 2.7% of them consume isot in powder form.
Frequency of isot consumption is as follows: every day (23.1%), once a week (21.6%) and once a
month (18.9%). On the other hand, thyme consumption frequency is 69.6% and participant generally
add thyme (89.4%) to their dishes. Thyme is often consumed once a week (28.9%) and its average
consumption amount is 1.220.68 tea spoon. 54.8% of the individuals use cinnamon and 57,8 of
cinnamon users prefer it in powder form. Additionally, cinnamon consumption (1.230.60 tea spoon)
frequency is once a month (33.0%), once a week (26.8%) and twice a week (12.7%). Locust
consumption frequency of the participants is 3.6% and they consume the tea of locust mostly (55.0%).
10.4% of the participants mostly add rosemary to their dishes (50.4%) and make tea of it (4.5%).
Rosemary is usually used once a month (29.4% - 1.350.75 tea spoon). Fennel use frequency of the
participants is 7.9%. In addition, fennel consumption frequency is once a month (38.2%), once a week
(18.0%) and twice a week (13.5%). 12.2% of the participants use basil and mostly add it to their dishes
(85.2%) and consume in powder form. Basil is monthly consumed once a month (40.1% - 1.680.99
tea spoon). 58.5% of the participants using coconut (33.4%) consume it in powder form. The
participants consume mostly once a week (33.0%) and average consumption amount is 1.601.18 tea
spoon. 71.4% of the participants consume mint (1.581.16 tea spoon) by adding the spice to their
dishes (90.2%), using it in powder form (7.2%) and making tea of the ground mint (2.6%).
Conclusion: This research showed that most of the spices are consumed in dishes and consumption
style, frequency and amount of each spice differs from each other.

KEYWORDS

Corresponding Author: DUYGU AAGNDZ, duyguturkozu@gazi.edu.tr

1
GAZ NVERSTES SALIK BLMLER FAKLTES BESLENME VE DYETETK BLM

1076
spice, nutrition, adults

1077
 Poster Session 8
Submission ID: 1008

DRUG INTERACTIONS WITH ST JOHNS WORT

1 1
GAMZE YURTDA , EFSUN KARABUDAK

ABSTRACT
St. John Wort (Hypericum perforatum L.) is a perennial plant belonging to the family
Hypericaceae, which grows in Europe, Asia, North Africa and the United States and has golden
blossoms of 30-90 cm height. In Turkey, it is known with the names such as union herb, sword herb,
lamb shredder, yellow centaur, wing herb (1). St. John's wort is indicated to have sedative and
astringent properties, and has been used traditionally for the treatment of excitability, neuralgia,
brositis, sciatica, menopausal neurosis, anxiety, depression and as a nerve tonic, and in topical
preparations for the treatment of wounds (2). It contains different groups of compounds such as
hypericin, hyperforin and flavonoides. Hypericin and hyperforin are suggested to be responsible for its
pharmacological activity (1, 2). Hyperforin induces cytochrome P450 3A4 (CYP3A4) and hypericin
induces the intestinal drug transporter P-glycoprotein (P-gp). These mechanisms might dramatically
affect the bioavailability of most of currently marketed drugs (3). St. John Wort has been shown to
lower the plasma concentration (and/or the pharmacological effect) of a number of drugs including
alprazolam, amitriptyline, cyclosporine, digoxin, fexofenadine, indinavir, irinotecan, methadone,
nevirapine, simvastatin, tacrolimus, theophylline, warfarin, phenprocoumon and oral contraceptives
(4). Induction of P-glycoprotein and/or cytochrome P450 (CYP) enzymes (particularly CYP 3A4) by
St. John Wort could explain such pharmacokinetic interactions. Combining St. John's wort with
serotonin selective re-uptake inhibitors and other antidepressants can cause serotonin syndrome and
therefore should be avoided (4). As a result, St. John Wort represents a herbal medicine with a high
potential for drug interactions. Some of such interactions may have serious clinical consequences.
Therefore, clinicians and patients should conscious of possible reductions in systemic bioavailability
of conventional drugs when taken together with St Johns wort. More research is require to ensure
reliable information to guide clinical practice. 1. Hl Y, ahin F, Omay S. Kantaronun (Hypericum
perforatum L.) bileimi ve tbbi nemi. International Journal of Hematology and Oncology.
2005;4(15):212-8. 2. Barnes J, Anderson LA, Phillipson JD. St John's wort (Hypericum perforatum
L.): a review of its chemistry, pharmacology and clinical properties. Journal of pharmacy and
pharmacology. 2001;53(5):583-600. 3. Gordon RY, Becker DJ, Rader DJ. Reduced efficacy of
rosuvastatin by St. John's Wort. The American journal of medicine. 2009;122(2):e1-e2. 4. Gezmen-
Karada M, Trkz D, Kapucu DT. Bitkiler ve ila etkileimleri.

KEYWORDS
Drug interaction, St. Johns Wort, Hypericum perforatum

Corresponding Author: GAMZE YURTDA, gmzyurtdas@hotmail.com

1
GAZ NVERSTES

1078
 Poster Session 8
Submission ID: 1010

VOLATILE OIL COMPOSITION OIL OF SCANDIX AUCHERI BOISS.

(APIACEAE) FROM TURKEY

1 1 1
AZZE DEMRPOLAT , GULDEN DOGAN , EYUP BAGC

ABSTRACT
Genus Scandix L. genus belonging to Apiaceae family is represented by nine taxons while
eight of them are species The analysis has led to the identification of 39 components comprising
98.0% of the oils. The essential oil yield was determined as 0.4 (v/w) and the main constituents of the
essential oil were germacrene-D (22.3%), spathulenol (11.5%), 1,5-epoxy-salvial-4(14)ene (6.4%) and
heptadecane(%6.2) and palmitic acid (5.7%). The essential oil S. aucheri has 40.2% sesquiterpenes,
33.8 % sesquiterpene hydrocarbons and 16.1% alkene and fatty acids. The results were compared with
the results of the genus patterns and discussed.in the Flora of Turkey. This study, reports the essential
oils composition of the aerial parts of Scandix aucheri Boiss. From Elaz/Turkey. The oil was
extracted by using Clevenger apparatus and analysed by GC and GC/MS system.

KEYWORDS
Scandix aucheri, Apiaceae, 9,12-Octadecanoic acid, n- Hexadecanoic acid, Natural Product.

Corresponding Author: AZZE DEMRPOLAT, azizetas3@hotmail.com

1
FIRAT UNVERSTES

1079
 Poster Session 8
Submission ID: 1011

CHEMICAL COMPOSITION SCANDIX PECTEN VENERIS L. AND

SCANDIX MACRORHYNCHA C.A. MEY (APIACEAE) ESSENTIAL
OIL

1 1
AZZE DEMRPOLAT , EYUP BAGC

ABSTRACT
The Scandix pecten veneris L. commonly known as Shepherds needle and S. macrorhyncha,
which belongs to Apiaceae. The S. pecten veneris leaves showed highest to moderate activity against
the studied microbial strains and anti-inflammatory. The present study revealed that the S. pecten-
veneris leaves could be the potential sources of balance diet with significant biological potentials. The
chemical composition of different parts of S. pecten veneris L. and S. macrorhyncha C.A. Mey.
essential oils naturally grown in Turkey were analyzed by GC and GC-MS system. The qualitative and
quantitative essential oil variation were also determined. The essential oil yields of S. pecten veneris
were found as 0.3(v/w) in aerial part and 0.2 (v/w) in fruits. 19 constituents were identified and
comprised 89.6 percentage of the total essential oil from S. pecten-veneris aerial parts, and identified
17 constituents comprised 87.7 % in fruit essential oil, respectively. The predominant compounds of
the aerial part oil of S. pecten-veneris, palmitic acid (28.2%), spathulenol (19.6%), 1,5-epoxy-salvial-
4(14)ene (7.2%) and caryophyllene oxide (5.4%) were the the main compounds and 9,12,
-curcumen (6.2%), caryophyllene oxide (5.4%) were the major
compounds in fruit. On the other hand, 10 constituents were identified and comprised 87.8 % of the
total essential oil of S. macrorhyncha aerial parts. The main compounds in oil were palmitic acid
(31.5%), 1-heptadecanol (25.2%), pentadecanoic acid (13.4%) ve 9-octadecanoic acid (12.6%). Fatty
acid, sesquiterpene and saturated hydrocarbons were determined as significant compounds for the
characterization of S. pecten-veneris and S. macrorhyncha essential oil. The results were discussed in
view of the chemotaxonomy and natural products.

KEYWORDS
Scandix pecten-veneris, S. macrorhyncha, Essential oil, palmitic acid, spathulenol, oleic acid.

Corresponding Author: AZZE DEMRPOLAT, azizetas3@hotmail.com

1
FIRAT NVERSTES

1080
 Poster Session 8
Submission ID: 1012

EFFECT OF NIGELLA SATIVE ON DYSLIPIDEMIA

1 1 1 1
GAMZE YURTDA , NILFER ACAR TEK , GAMZE AKBULUT , TUBA TATAR

ABSTRACT
Dyslipidemia is a wide term covering diverse lipid and/or lipoprotein abnormalities [1]. The
main findings of this disorder include elevated plasma concentrations of total cholesterol (TC), low-
density lipoprotein cholesterol (LDL-C) and triglycerides (TG), and low concentrations of high-
density lipoprotein cholesterol (HDL-C) [1]. Dyslipidemia is an important risk factor for
cardiovascular disease, the leading cause for morbidity and mortality among patients [2]. There is
growing interest in finding safe natural alternatives to common drugs used to treat dyslipidemia,
specially in patients resistant to or intolerant of statins [3]. Nigella sativa (N. sativa), popularly known
as black seed, is one of these safe plants used as an herb for more than 2000 years and has been shown
to produce multi-systemic beneficial actions, including hypoglycemic, hypocholestermic, and
antioxidant effects. It contains numerous biologically active constituents such as thymoquinone (TQ)
,flavonoids, sterols, and polyunsaturated fatty acids and the lipid-lowering effect is likely mediated by
a synergistic action of its different components [3]. Several studies on N. Sative demonstrated
improvement in serum lipid levels including decrease in total lipids, TG, low-density lipoprotein
(LDL) and increase in high-density lipoprotein (HDL) levels [4, 5]. For instance, dietary
supplementation of N. Sativa seeds (400600 mg/ kg) for 1, 2 or 4 weeks is useful in the prevention
and treatment of the hyperlipidemia and hypercholesterolemia [5]. Bhatti et al. indicated that oral
administration of powdered black seeds (1g/day) to hypercholesterolemic patients for 2 months
significantly decreased the total cholesterol, TG, and LDL-C levels and increased the HDL-C level [6].
Bamosa et al. observed a important effect of six different doses of thymoquinone on blood lipids in
rats [7]. The effects dyslipidemic of N. sativa and TQ may be related to the significant decrease in
hepatic HMG-CoA reductase activity, increase in arylesterase activity, regulatory effects on genes that
influence cholesterol metabolism, as well as antioxidant mechanisms [4]. As a result, Nigella sativa
and thymoquinone appear as effective and safe natural treatments for patients with dyslipidemia.
References 1. Asgary S, Sahebkar A, Goli-Malekabadi N: Ameliorative effects of Nigella sativa on
dyslipidemia. Journal of endocrinological investigation 2015, 38(10):1039-1046. 2. Butt MS, Sultan
MT: Nigella sativa: reduces the risk of various maladies. Critical reviews in food science and nutrition
2010, 50(7):654-665. 3. Sahebkar A, Beccuti G, Simental-Menda LE, Nobili V, Bo S: Nigella sativa
(black seed) effects on plasma lipid concentrations in humans: A systematic review and meta-analysis
of randomized placebo-controlled trials. Pharmacological research 2016, 106:37-50. 4. Razavi B,
Hosseinzadeh H: A review of the effects of Nigella sativa L. and its constituent, thymoquinone, in
metabolic syndrome. Journal of endocrinological investigation 2014, 37(11):1031-1040. 5. Kocyigit
Y, Atamer Y, Uysal E: The effect of dietary supplementation of Nigella sativa L. on serum lipid
profile in rats. Saudi medical journal 2009, 30(7):893-896. 6. Bhatti I, Rehman FU, Khan M, Marwat
S: Effect of prophetic medicine Kalonji (Nigella sativa L.) on lipid profile of human beings: an in vivo
approach. World Applied Sciences Journal 2009, 6(8):1053-1057. 7. Bamosa AO, Ali BA, al-

Corresponding Author: GAMZE YURTDA, gmzyurtdas@hotmail.com

1
GAZ NVERSTES

1081
Hawsawi ZA: The effect of thymoquinone on blood lipids in rats. Indian journal of physiology and
pharmacology 2002, 46(2):195-201.

KEYWORDS
nigella sative, dislipidemia, black seed

1082
 Poster Session 8
Submission ID: 1013

THE BIOLOGICAL ACTIVITIES AND ESSENTIAL OIL

COMPOSITION OF SALVIA KURDICA

1 2 3 3 4
HILAL SARUHAN FIDAN , IIL AYDIN , ESRA YARI , EVIN AYGN TUNCAY , GKHAN ZENGIN , MEHMET
5 6 3
FIRAT , MEHMET BOA , ABDULSELAM ERTA

ABSTRACT
The genus Salvia L. (Lamiaceae) comprises about 900 species world-wide, while it is
presented with 89 species and 94 taxa in Turkey, approximately half of which are endemic. Anatolia is
the major gene center in Asia. Salvia species, known as adacayi in Anatolia, are used in folk
medicine for the treatment of a variety of diseases, including infectious diseases. They are used as
antiseptics, stimulants, diuretics and for wound healing in Turkish folk medicine and for herbal teas.
Salvia fruticosa and Salvia tomentosa, which have similar chemical composition and effects with the
medicinal species (Salvia officinalis L.), are preferred in Turkey beside of S. officinalis. The essential
oil of S. fruticosa is used traditionally as carminative, stomachic, antiperspirant and diuretic. Due to
several studies indicating antimicrobial, antifungal and antioxidant activities of Salvia species,
especially S. officinalis, similar studies on these species increased gradually all over the world. The
aim of this study was to determine the essential oil profile of Salvia kurdica by GC/MS. Additionally,
the essential oil of this Salvia was tested for antioxidant (DPPH, Beta Caroten Cuprac, ABTS) and
anticholinesterase activities. The main constituents of S. kurdica were identified as Linalool (26.4%),
Geranyl acetate (22.4%) and trans-Linalool oxide (furanoid) (9.1%). The essential oil of S.kurdica
exhibited moderate antioksidant and good anticholinesterase activities. Acknowledgements: The
research was funded by grant : KBAG 114Z801 from TUBITAK, The Scientific and Technological
Research Council of Turkey.

KEYWORDS
Salvia kurdica, Essential Oil, Antioksidant

Corresponding Author: ABDULSELAM ERTA, abdulselamertas@hotmail.com

1
DCLE UNVERSTY, FACULTY OF PHARMACY, DEPARTMENT OF BOCHEMSTRY, DYARBAKIR
2
DCLE UNVERSTY, FACULTY OF PHARMACY, DEPARTMENT OF ANALYTCAL CHEMSTRY, DYARBAKR
3
DCLE UNVERSTY, FACULTY OF PHARMACY, DEPARTMENT OF PHARMACOGNOSY, DYARBAKIR
4
SELUK UNVERSTY, FACULTY OF SCENCE, DEPARTMENT OF BOLOGY, KONYA
5
YZNC YIL UNVERSTY, FACULTY OF EDUCATON, DEPARTMENT OF BOLOGY, VAN,TUKEY
6
DCLE UNVERSTY, FACULTY OF PHARMACY, DEPARTMENT OF PHARMACEUTCAL TECHNOLOGY,
DYARBAKIR

1083
 Poster Session 8
Submission ID: 1014

THE ESSENTIAL OIL ANALYSIS OF SOME SALVIA SPECIES FROM

ANATOLIA WITH CHEMOMETRIC APPROACH

1 2 2 3
ZGE TOKUL LMEZ , ABDULSELAM ERTA , HILAL SARUHAN FIDAN , MEHMET FIRAT , MUSTAFA ABDULLAH
4 5 1 4 6
YILMAZ , GKHAN ZENGIN , MEHMET ZTRK , HAMDI TEMEL , UFUK KOLAK

ABSTRACT
The genus Salvia L. a member of the Lamiaceae family grows naturally all over the world
with more than 900 species. Lamiaceae family have been interested since many of the Lamiaceae
species include essential oils which are used in perfumery and pharmaceutical industries. Medicinal
plants having biological activity have been used in the treatment of a variety of diseases since ancient
times. PCA is one of the best multivariate statistical techniques for extracting linear relationships
among a set of variables. PCA is a set of widely used analytical techniques whereby a complex dataset
containing variables is transformed to a smaller set of new variables, which maximize the variance of
the original dataset. PCA provides information on the significant parameters with minimum loss of
original information. This is achieved by transforming to a new set of variables which are
uncorrelated, and which are ordered so that the first few retain most of the variation present in all of
the original variables. The principal components are generated in a sequentially ordered manner with
decreasing contributions to the variance, i.e. the first principal component (PC1) explains most of the
variations present in the original data, and successive principal components account for decreasing
proportions of the variance. In this study, Principle Component Analysis (PCA) and Hierarchical
Clustering Analysis (HCA) were performed with 13 variables in 8 samples. As a result of the PCA
analysis with 8 samples and 13 volatile oil components, the first three principal components explained
the variance as 85.3%, 1st principle component as 44.6% and 2nd. principle component as 28.1%.
Statistical calculations were performed using Minitab 16.2.1 statistical software (MINITAB Inc.
2010). Salvia species collected at different times in the study were evaluated with 13 volatile oil
components.

KEYWORDS
Salvia, Chemometri, Essential Oil, PCA

Corresponding Author: ABDULSELAM ERTA, abdulselamertas@hotmail.com

1
MUGLA SITKI KOMAN UNVERSTY, FACULTY OF SCENCE, DEPARTMENT OF CHEMSTRY, TR-48121
MUGLA,
2
DCLE UNVERSTY, FACULTY OF PHARMACY, DEPARTMENT OF PHARMACOGNOSY, TR-21280
DYARBAKIR,
3
YZNC YIL UNVERSTY, FACULTY OF EDUCATON, DEPARTMENT OF BOLOGY, TR-65080 VAN,
4
DCLE UNVERSTY, FACULTY OF PHARMACY DEPARTMENT OF PHARMACEUTCAL CHEMSTRY TR-
21280 DYARBAKIR,
5
SELUK UNVERSTY, FACULTY OF SCENCE, DEPARTMENT OF BOLOGY, KONYA,
6
DEPARTMENT OF GENERAL AND ANALYTCAL CHEMSTRY, FACULTY OF PHARMACY, ISTANBUL
UNVERSTY, TURKEY

1084
 Poster Session 8
Submission ID: 1016

DETERMINATION OF CONSTANT OIL COMPONENTS OF NIGELLA

ARVENSIS VAR. GLAUCA SPECIES NATURALLY GROWING IN
FLORA OF KAHRAMANMARAS

1 2 1 1 1
OSMAN GEDK , YUSUF ZIYA KOCABA , MER SHA USLU , ALI RAHMI KAYA , ADEM EROL , MUHAMMAD
3 4
AZIM KHAN , WALID SOUFAN

ABSTRACT
Black sesame (Nigella sp.) is a one-year herbaceous plant belonging to the family
Ranunculaceae (wedding flowers). Its origins are based on the Eastern Mediterranean, Southern
Europe and West Asia. The genus Nigella is represented by about 20 species from the Mediterranean
region to Western Asia and comprises about 13 species in Turkey. There is %32-40 fixed fat, %16-
19.90 protein, %33.90 carbohydrate, %5.50 saponins, alkaloids and fibers, %1.79-3.44 tannins and
minerals in the seeds of Turkey, as well as differences according to the regions. In fixed oil,
unsaturated fatty acids include linoleic acid, oleic acid and linolenic acid while saturated fatty acids
include palmitic acid, stearic acid and myristic acid. N. arvensis var. glauca is spreading naturally in
Kahramanmaras Sutcu Imam University Avar Settlement. In this study totally twenty different fatty
acid components was determined. Primarily fixed fatty acids components and ratios of the species N.
arvensis var. glauca; linoleic acid (%68.11), oleic acid (%12.00), palmitic acid (%10.88), cis-11,14-
eicosadienoic acid (%2.90), stearic acid (%2.36), gamma- linolenic acid (%1.59), myristic acid
(%0.23) were detected.

KEYWORDS
Black sesame, Nigella, fixed oil, fatty acid components

Corresponding Author: OSMAN GEDK, ogedik@ksu.edu.tr

1
ST MAM NVERSTES ZRAAT FAKLTES
2
ST MAM NVERSTES TRKOLU MYO
3
THE UNVERSTY OF AGRCULTURE
4
KNG SAUD UNVERSTY

1085
 Poster Session 8
Submission ID: 1017

SOME VEGETABLE OILS USED IN WOUND CARE AND THEIR

EFFECTS ON WOUND HEALING

1 1
FIGEN EROL URSAVA , SEHER GNEN ENTRK

ABSTRACT
Aromatherapy, a complementary treatment alternative, refers to use of essential oils derived
from different parts of plants (leaf, flower, bark, seed, fruit etc.) for health and wellbeing purposes.
These oils are applied through respiratory tract, skin and oral route. It is known that essential oils are
utilized in mother and child health, pain management, care for cancer patients, treatment of skin,
neurological, respiratory and gastrointestinal diseases, wound healing/care and mental health. It has
been shown in the literature that essential oils used in wound care accelerates healing. Health
professionals play an important role in development and use of wound care products containing
essential oils which quicken wound healing. Purpose of this study is directed towards examination of
essential oils which quicken wound healing and their uses, at the same time, to create awareness in this
matter. It has been shown in the literature that essential oils (tea oil, lavender oil, canola oil, St. Johns
Wort oil, olive leaf extract, beeswax-olive oil) have effects on wound healing. Tea oil has
antimicrobial features. Wound dressing with tea tree oil every day three times a week has been found
to have effects on healing of MRSA positive wounds. Episiotomy is a surgical incision made during
vaginal delivery. A study comparing bathing with 5-6 drips of lavender oil in 4-liter water and bathing
with povidone iodine for 10 days did not reveal any differences in terms of wound healing
complications, but showed less redness in episiotomy wounds exposed to lavender oil. In a study
examining effectiveness of canola oil and lavender honey in wounds created in rats, canola oil applied
through a syringe two times for the first four days was found to be more effective than lavender honey
applied in the same way. In a study on effects of a wound care product containing beeswax, olive oil
and alkanna tinctoria on the second-degree burns, wound dressing was made with the product every
day and it was found to be effective. St. Johns Wort is a plant used in treatment of several diseases.
Applying St. Johns Wort on wounds created on rats once daily was found to be effective in soft tissue
defects. Olive leaves have tannin, essential oils, organic acids and resin. Using topical olive oil extract
on diabetic wounds in rats for 21 days was shown to accelerate wound healing.
Aromatherapy/essential oil use, a complimentary therapy, plays a part in improving health and
wellbeing and influences nursing care having a key role in wound care. In studies conducted that
essential oils have been found to have a beneficial effect on wound care. Studies have revealed that
they have impact on wound healing. In light of the literature, it can be recommended that randomized
controlled studies should be performed to evaluate effects of wound care products made from essential
oils on types of wound in Turkey.

KEYWORDS

Corresponding Author: FGEN EROL URSAVA, figenerolll@gmail.com

1
ANKIRI KARATEKN UNVERSTY, FACULTY OF HEALTH SCENCES, DEPARTMENT OF NURSNG

1086
Wound care, Herbal oil, Aromatherapy

1087
 Poster Session 8
Submission ID: 1018

ANTICARCINOGENIC EFFECTS OF FERMENTED WHEAT GERM

EXTRACTS

1 2
HAKAN VATANSEV , MUSTAFA KRAT DEMR

ABSTRACT
Fermented wheat germ extract (FWGE) is a natural product obtained by fermentation of wheat
germ and registered as a special nutrient in Hungary for cancer patients in 2002. According to some
researches in recent years, quinones found as glycosides in wheat germ are thought to be responsible
for some biological properties of FWGE. FWGEs production process includes fermentation of this
extract by Saccharomyces cervisiae, separation, drying and granulation of the fermentation liquid.
FWGE is available without prescription from pharmacies and the effects are versatile. They are
reported to show no toxicity, mutagenicity or genotoxicity. In particular, the role of cancer prevention
and treatment has come to the forefront with its various aspects. Compared to normal tissues, cancer
cells exhibit a hypermetabolic state with high amounts of glucose use while FWGEs inhibit cancer
growth by inhibiting glucose uptake in cancer cells. In cell culture studies in which the antitumoral
effect of FWGE is examined; It has been demonstrated that they have potential antitumoral activity in
the colon, testis, thyroid, ovary, non-small cell lung, breast, gastric, head and neck, hepatoma,
glioblastoma, neuroblastoma, melanoma, cervical cancer cell lines.

KEYWORDS
FWGE, cancer, antitumoral effect.

Corresponding Author: HAKAN VATANSEV, hakanvatansev@gmail.com

1
NECMETTN ERBAKAN NVERSTES SEYDEHR MESLEK YKSEKOKULU, UN VE UNLU MAMULLER
TEKNOLOJS, KONYA
2
NECMETTN ERBAKAN NVERSTES MHENDSLK VE MMARLIK FAKLTES, GIDA MHENDSL
BLM, KONYA

1088
 Poster Session 8
Submission ID: 1019

THE ANTICHOLINESTERASE ACTIVITY AND TOTAL PHENOLIC

CONTENT OF ESSENTIAL OILS OF SOME SALVIA SPECIES FROM
ANATOLIA

1 1 2 3 4
HATICE AKIRCA , ABDULSELAM ERTA , MEHMET FIRAT , IIL AYDIN , ESRA YARI , MEHMET VEYSI
4 3 3 5 6
ALAYAN , MEHMET AKDENIZ , SMAIL YENER , HSEYIN ALKAN , GLATI TOPU

ABSTRACT
Salvia L. (Lamiaceae) species consists of about 900 species distributed throughout the world.
In Turkey, this genus is represented by 100 species, 53 (53%) of which are endemic. The genus is
named Salvia, derived from latin Salveo, means save, or recover. Many of Salvia species are
named adaay in Anatolia, Turkey, and used as herbal tea due to their antiseptic, stimulant,
diureticand wound healing properties. Salvia species are generally known for their multiple
pharmacological effects including their antibacterial, antiviral, antioxidative, antimalarial, anti-
inflammatory, antidiabetic, cardiovascular, antitumor and anticancer. Also, some studies showed that a
part of these activities depended on essential oil composition. The essential oils of some Salvia species
were tested for anticholinesterase (Acetyl- and butrylkolinesterase enzymes) activities and total
phenolic content in our study. Essential oil samples were obtained by a Clevenger apparatus from the
whole parts of plants which were crumbled into small pieces and soaked in distilled water for 3 h.
Then, these samples were dried over anhydrous Na2SO4 and stored at +4C for a sufficient period of
time. The essential oils of studied four Salvia species were determined the similar results as total
phenolic content. The acetyl- and butrylkolinesterase enzyme activities of S. macrochlamys
(88,683,60 and 104,344,25 respectively) were determined as high. The essential oils of S. sclarea
and S. palaestina were showed a good activity in butrylkolinesterase enzyme.

KEYWORDS
Salvia sclarea, Salvia macrochlamys, Salvia hypargeia, Salvia palaestina, Essential Oil,
Anticholinesterase

Corresponding Author: MEHMET VEYS ALAYAN, mehmetveysicaglayan@hotmail.com

1
DCLE UNVERSTY, FACULTY OF PHARMACY, DEPARTMENT OF ANALYTCAL CHEMSTRY,
DYARBAKR, TURKEY
2
YZNC YIL UNVERSTY, FACULTY OF EDUCATON, DEPARTMENT OF BOLOGY, VAN, TURKEY
3
DCLE UNVERSTY, FACULTY OF PHARMACY, DEPARTMENT OF ANALYTCAL CHEMSTRY,
DYARBAKR, TURKEY
4
DCLE UNVERSTY, FACULTY OF PHARMACY, DEPARTMENT OF PHARMACOGNOSY, DYARBAKIR,
TURKEY
5
DCLE UNVERSTY, FACULTY OF PHARMACY, DEPARTMENT OF BOCHEMSTRY,DYARBAKIR, TURKEY
6
DEPARTMENT OF PHARMACOGNOSY AND PHYTOCHEMSTRY, BEZMALEM VAKF
UNVERSTY,ISTANBUL, TURKEY

1089
 Poster Session 8
Submission ID: 1021

ANTIFUNGAL EFFECTS OF SOME PLANT EXTRACTS ON KASHAR

CHEESE

1 2 1
GKHAN AKARCA , MUHAMMED YUSUF ALAR , AYIN KAHRAMAN

ABSTRACT
Cheese, which is a milk product rich in protein, fat and minerals, has had a significant place in
the diet of all societies for centuries. A large number of varieties of cheese are produced and consumed
in our country. One of them is the kashar cheese which is in the cake-filata class. In its production,
there are production steps such as fermentation, coagulation, ripening of the clot, processing and
salting. After these stages, it has a long ripening phase so that it can possess its sensory, physical and
chemical properties until the consumption phase. During this ripening phase, the surface of the cheese
is completely covered with mold. Mold growth on the surface causes quality loss, discoloration, bad
odor and aroma disorders. Besides, due to the toxic metabolites, mycotoxins, which they develop on
the surface, molds can lead to poisoning which can result in death when the cheese is consumed.
Although these toxins are more dense in the 1-2 cm surface layer of the cheese, they can also migrate
to the interior. For this reason, the problem of mold must be avoided. Various methods are used to
solve this problem. One of these methods is to try to mechanically clean the crust layer by chewing
with a brush and water and sometimes even with a knife while the cheese is being consumed.
However, this process can not solve the health and quality problems caused by molds. Other methods
of preventing molds are known to be the use of preservatives (antifungal) and special packaging
methods. The use of antifungal agents is intended to prevent quality losses and deterioration reactions,
prolong shelf life and maintain sensory properties. For this purpose various plant extracts are used as
antifungal agents. Within the scope of the study, the fresh kashar cheese produced is covered with
prepared sage, locust, thyme, cinnamon and ginger alcohol extracts. The study was carried out with 6
designation (control, sage, carob, thyme, cinnamon and ginger extracts x 3 time (day 0, day 7, day 15)
trial design. At the end of storage, the number of yeast-molds on the surfaces of the samples was
determined. According to the obtained data, it is thought that the plant extracts used prevent the
growth of mold on the surface of kashar cheese and it is advisable to use vacuum packaging as an
alternative packaging material for covering the natural plants with kashar cheeses.

KEYWORDS
Kashar cheese, molding, plant extract, coating

Corresponding Author: GKHAN AKARCA, gakarca@aku.edu.tr

1
AFYON KOCATEPE NVERSTES MHENDSLK FAKLTES GIDA MHENDSL BLM
2
STANBUL SABAHATTN ZAM NVERSTES, MHENDSLK VE DOA BLMLER FAKLTES, GIDA
MHENDSL BLM

1090
 Poster Session 8
Submission ID: 1022

SAGE (SALVIA OFFICINALIS L.)

1 1 1
TESLIME EKZ , OKTAY TOMAR , MEHMET KILIN

ABSTRACT
The Salvia species belonging to the Lamiaceae family consist of approximately 900 species
that are common worldwide and exhibit significant morphological and genetic diversity relative to
their geographical origins. In many studies, it has been reported that sage species have
pharmacological properties such as antioxidant, antiinflammatory, analgesic, antipyretic, hemostatic,
hypoglycemic and antitumor effects. Salvia officinalis L., which is called as medical sage, is a plant
with fringing roots and a length of 60-100 cm. Leaves are whitish gray and silver colored and furry.
This aromatic plant contains essential oil. Among people in our country; it is widely consumed in the
form of herbal tea because it is thought to have some important medical effects such as cutting nausea,
reducing abdominal pain, digestion regulation, relieving rheumatic pain, improving tongue and
gingivitis, protecting liver, cleansing blood and lowering blood pressure. Apart from that, cosmetics,
perfumery, pharmaceutical industry are in use. In food industry, besides giving flavor to products, it is
a spice with widespread use as an alternative to synthetic and chemical antimicrobial and antioxidant
substances.

KEYWORDS
Sage, Salvia officinalis L., antimicrobial, antioxidant

Corresponding Author: OKTAY TOMAR, oktomar@aku.edu.tr

1
AFYON KOCATEPE NVERSTES MHENDSLK FAKLTES GIDA MHENDSL BLM

1091
 Poster Session 8
Submission ID: 1023

USE OF OCIMUM BASILICUM L. IN FUNCTIONAL FOOD

PRODUCTION

1 1
MEHMET KILIN , TESLIME EKZ

ABSTRACT
The Ocimum genus contains over 150 species and is considered the largest genus of the
Lamiaceae family. Ocimum spp. contains essential oils rich in phenolic compounds and other natural
products in a wide range including polyphenols such as flavonoids. This genus Ocimum basilicum L.
species is a important spice and essential oil plant that grows in various parts of the world. This
medicinal and aromatic plant is originated from North East Africa, North West India and Central Asia.
Among the people in our country is known as fesleen and reyhan. The length of grown up basil is
usually between 20-60 cm. It has soft leaves in the length of 1-5 cm, width of 1-3 cm. There are many
basil varieties which vary according to leaf color (green or purple), flower color (white, red, purple)
and aroma. There are oil grands forming characteristic aromatic smell in plant leaves and flowers.
Basil contains 0.2-1% essential oil and the content of essential oils in different basil cultures is also
different. Along with essential oil, it is reported that this plant contains 14% protein, 6.1%
carbohydrates and high vitamin C and vitamin A content. Fresh flowering branches and seeds of this
plant, which is cultivated and traded in our country, are used. Basil, which has high aromatic character,
is widely used both as fresh and dried food spice in Turkish cuisine. Apart from this, the plant also
finds use in food products, oral care products and cosmetic industry. Among people in our country; it
is consumed for various purposes such as gas remover, appetizer, and digestion facilitator. In addition,
in some studies, it has been reported that basil leaves have tonic, antiseptic and insecticidal properties
and this plant has antiviral, antimicrobial and antioxidant activities. In addition to these mentioned
properties, by examining the benefits of the proprietary aroma especially for human health; It is
thought to be an alternative plant for the production of new functional food products. There are
various studies on the use of basil in functional food products and it is considered that different studies
should be done in the development of new functional food products.

KEYWORDS
Functional food, Ocimum basilicum L., spice, human health

Corresponding Author: TESLME EKZ, tekiz@aku.edu.tr

1
AFYON KOCATEPE NVERSTES MHENDSLK FAKLTES GIDA MHENDSL BLM

1092
 Poster Session 8
Submission ID: 1024

PROPERTIES OF ALKANNA TINCTORIA L. TAUSCH PLANT

1 1
MEHMET KILIN , AHMET FURKAN ALAR

ABSTRACT
Alkanna tinctoria L. Tausch is a perennial plant belonging to the Boraginaceae family. It is
also known as "alkanet" among the people. This plant has 10-30 cm long and blue flowers. A. tinctoria
is used in the pharmaceutical industry and in cosmetics. Collected and dried roots of this plant are used
in herbal dyeing. Alkaline and shikonin, pigmentary substances, were isolated from the root of the this
plant. Alkannin and shikonin are powerful pharmaceutical ingredients with a wide range of biological
properties and are used as food colorants. It has been reported that alkannin, shikonin and its
derivatives may exhibit antioxidant properties in fat substrates as well as wound healing,
antiinflammatory, antitumor and antimicrobial properties. It is necessary to investigate the possibility
of functional use of this plant in food production processes in particular by taking advantage of the
antimicrobial effect.

KEYWORDS
Alkanna tinctoria L., alkanet, antimicrobial activity, food

Corresponding Author: MEHMET KILIN, mkilinc@aku.edu.tr

1
AFYON KOCATEPE NVERSTES MHENDSLK FAKLTES GIDA MHENDSL BLM

1093
 Poster Session 8
Submission ID: 1025

USE OF SOME PLANTS WITH ANTIBACTERIAL AND ANTIFUNGAL

PROPERTIES IN THE CONSERVATION OF SUCUK

1 1
TESLIME EKZ , OKTAY TOMAR

ABSTRACT
Meat is a valuable food material with its nutrients such as minerals, vitamins, essential fatty
acids and its odor, taste and aroma properties. Thanks to these nutritious ingredients, it has an
extremely important place among the elements of balanced nutrition in human life. Due to its high
moisture content, nitrogenous nutrients, minerals and other growth factors, the meat becomes suitable
for many microorganisms to grow rapidly and for deterioration in a short time. Since ancient times,
various methods have been used, such as drying and fermentation processes, in order to impart
different flavor and aroma to the meat and increase the period of preservation of the meat which is
degradable in a short time. Thus, many meat products appeared in different flavor, structure, color and
appearance. One of these products is sucuk. Sucuk is an important meat product that has been
produced and consumed in our country since ancient times. Various microbial spoilage can be seen in
sucuks, because of the reasons like; having high pH and moisture content after production, not being
transported under cold chain and not being kept in cold. Mold growth on sucuk surface, which is one
of these microbial spoilages, is a serious problem in the industry. Chemical preservatives are
frequently used in the food industry to prevent this problem. But, the consumer's skeptical attitude
towards chemical additives and their interest in natural functional food products are increasing day by
day. As a result, efforts are being made to search for natural antimicrobial agents that can be used to
prevent microbial spoilage and extend shelf life. For this purpose, various plants such as Thymus
vulgaris L., Ocimum basilicum L., Cuminum cyminum L., Origanum majorana L., Foeniculum
vulgare Miller., Terminalia chebula Retz. extracts, extract mixtures, and essential oils have been
investigated for their antibacterial and antifungal effects. The increasing demand for natural and
healthy food reveals the necessity of carrying out more detailed scientific studies in the following
period.

KEYWORDS
Antibacterial, antifungal, sucuk, chemical preservatives, food preservation

Corresponding Author: TESLME EKZ, tekiz@aku.edu.tr

1
AFYON KOCATEPE NVERSTES MHENDSLK FAKLTES GIDA MHENDSL BLM

1094
 Poster Session 8
Submission ID: 1026

NATURAL PHARMACY: CLOVE (SYZYGIUM AROMATICUM)

1 1 1
OKTAY TOMAR , MEHMET KILIN , ABDULLAH ALAR

ABSTRACT
Clove (Syzygium aromaticum) is one of the oldest and most valuable spices in the world and
is among the most traded medical and aromatic plants. The clove which the Southern Philippines and
the Molluk Islands are its mainland, are cultivated in all tropical countries today and are produced
mostly in Indonesia, Tanzania's Zengibar and Pemba Islands. Clove has a strong aroma, sharp smell,
burning, bitter pungent flavor; it contains 15-20 % volatile oil. The main component of this volatile oil
is eugenol, which is present in about 75-90% of the main component in the phenolic structure, which
gives the typical aroma of spring to its therapeutic properties. Eugenol in the clove is a phenolic
substance giving antiseptic, bacteriostatic, bactericidal, analgesic, antifungal properties. Clove is
mainly used in food, pharmaceutical and also widely used in the fields of perfumery and cosmetics
fields. Many scientific studies have been conducted on cloves; they show that it is a medicinal and
aromatic plant which is effective in various fields such as antiseptic, antifungal, antiviral, local
anesthetic, antioxidant, antimicrobial, antithrombotic, antiinflammatory, anticarcinogenic, diarrhea,
digestive system disorders. It is also used in the treatment of many diseases such as ulcers, wounds,
arthritis, rheumatism, sprains, asthma, bronchitis, nausea, minor infections and antispasmodia in dental
infections, mouth perfumes and toothpastes in folk medicine. In the past, clove has been used in the
treatment of many diseases among the public, and also many medical features have been discovered
today. It is thought that clove is a natural and effective alternative to synthetic drugs by carrying out
more scientific studies on these properties of human health. It is also widely used in the food industry
to enhance the flavor and aroma of food. On the other hand, there is a need for new research on cloves
to ensure food safety in food production processes and to increase the functionality of food.

KEYWORDS
Clove, eugenol, medical, aromatic, human health

Corresponding Author: OKTAY TOMAR, oktomar@aku.edu.tr

1
AFYON KOCATEPE NVERSTES MHENDSLK FAKLTES GIDA MHENDSL BLM

1095
 Poster Session 8
Submission ID: 1027

ROSELLE (HIBISCUS SABDARIFFA L.): EFFECTS ON HEALTH

1 1 1
SEDEF AYDIN , GKHAN AKARCA , DUYGU ERCAN ORU

ABSTRACT
About 300 species of the genus Hibiscus are used in various applications. Roselle belongs to
the family Malvaceae and is one of the most consumed species of hibiscus. It is an important annual
crop grown in tropical and subtropical climates, called 'kerkeda' in the local area. Hibiscus sabdariffa
L. is used in food, animal feed, cosmetics, nutraceuticals and medicines. It is widely consumed in the
form of herbal tea, jelly and jam. The bright red color and unique flavor make it a valuable product.
The most important part of the plant is considered to be the sepals surrounding the fruit. Calycles,
stalks and leaves have sour aroma. It is thought that fruit juice obtained from sepals is a healthy drink
because of high vitamin C, anthocyanin and antioxidant content. Seeds are low in cholesterol, other
phytosterols and tocopherols in -sitosterol and -tocopherol. Pharmacological investigations of the H.
sabdariffa L. line revealed the biological activity of this plant. Some of the therapeutic effects of
consuming Roselle, especially in tea form are antioxidant, anticarcinogen, antipyretic, antidiarrheal,
antiinflamatuar, antitumor, antibacterial, antifungal, antiparasitic, anticholesterol effects, digestive
regulator and support for kidney function. The fact that it is rich in anthocyanins makes gives this
plant a good antioxidant source as well as gives it a good color appearance. Roselle plant's numerous
medical applications are developing worldwide. The biological activity of anthocyanins such as
antioxidant activity, which protects against atherosclerosis and anticarcinogenic activity, has been
investigated and found to have positive effects on the treatment of diseases. Besides, it has been
determined that the sepal extract of Hibiscus sabdariffa plant has therapeutic effect against leukemia
due to high polyphenol content. The result of phytochemical and pharmacological studies is that
Roselle has a positive effect on the treatment of many diseases, suggesting that the work to be done on
this field will be carried out on a broader scale.

KEYWORDS
Hibiscus sabdariffa L., roselle, medical and aromatic, pharmaceutical, health

Corresponding Author: SEDEF AYDIN, sedefaydin03@gmail.com

1
AFYON KOCATEPE NVERSTES MHENDSLK FAKLTES GIDA MHENDSL BLM

1096
 Poster Session 8
Submission ID: 1028

AVOCADO'S COMPOSITION AND POSITIVE EFFECTS ON HEALTH

1 1 2
GKHAN AKARCA , SEDEF AYDIN , MUHAMMED YUSUF ALAR

ABSTRACT
Avocado (Persea americana Mill.) is a green and perennial plant belonging to the family
Lauraceae, which is produced in about 50 countries, including the USA and Mexico. The avocado fruit
is considered as 'functional food' because of its beneficial effects on health. Avocado's oil has great
importantance on the occurance of its own flavor and aroma. It has a similar chemical composition
with olive oil which has an important place in Mediterranean diet. Studies have shown that avocado
oil contains higher levels of vitamin C, -sitosterol and chlorophyll, and lower levels of squalene and
polyphenol than olive oil.Avocado, an essential nutrient and serving as an exogenous antioxidant,
contains more lipolytic extract, C and E vitamins than other fruits. In addition, it contains
monounsaturated fatty acids, folate and B6 vitamins and phytosterols, which reduce the level of low-
density lipoprotein cholesterol (LDL) in the blood. These and similar compounds obtained from
avocado avocado oil and pulp are functioning as bioactive substances. Avocado, which is among the
fruits that have the highest concentration of phytosterols, assumed as the major bioactive group, is
especially rich in -sitosterol, the main sterol. The most apparent effect of phytosterols on
cardiovascular disorders has been determined to cause a decrease in cholesterol levels through the
inhibition of intestinal fat. In vitro and in vivo studies have been found that increased consumption of
avocado on a daily diet significantly reduce the risk of cardiovascular disease, osteoarthritis, cancer,
obesity, and inflammation. In addition, it has been reported that D-Mannoheptulose (MH), which is
contained in avocadonin, has an aging-retarding effect and is used as a potential treatment for
hypoglycemia. Given the positive health effects of avocado-enriched diets, studies using avocado and
avocado-derived products are predicted to accelerate significantly in the coming years.

KEYWORDS
Avocado, functional food, disease, cardiovascular, bioactive, health

Corresponding Author: SEDEF AYDIN, sedefaydin03@gmail.com

1
AFYON KOCATEPE NVERSTES MHENDSLK FAKLTES GIDA MHENDSL BLM
2
STANBUL SABAHATTN ZAM NVERSTES, MHENDSLK VE DOA BLMLER FAKLTES, GIDA
MHENDSL BLM

1097
 Poster Session 8
Submission ID: 1030

THE BENEFITS OF BLACKCURRANT (RIBES NIGRUM L.) FOR

HEALTH

1 1 1
ABDULLAH ALAR , HLYA VATANSEVER , ELIF EKMEK

ABSTRACT
Blackcurrant (Ribes nigrum L.) which is native to Europe and Northern Asia is a perennial
plant with edible, small, dark purple (near-black) colorful fruits. Its fruits and leaves have been used
for a variety of health problems in traditional plant medicine in Europe and Asia. Blackcurrant is a rich
source of anthocyanins, which are vitamin C and natural food coloring. It is also known that it has a
higher antioxidant capacity compared to other widely consumed berry fruits. Black currant seeds are
rich in -linolenic acid and -linolenic acid, which play an active role in growth, healthy bone
development, development of normal brain function and regulation of metabolism. Blackcurrant fruits
contain polyphenolic compounds with antioxidant, anticarcinogenic, antibacterial, antimicrobial and
antiviral properties. Thanks to these properties of polyphenols, many functions of the organs and
especially the nervous system, digestive system and circulatory system are protected and supported.
Anthocyanins (delphinidin-3-glucoside, delphinidin-3-rutinocyte, cyanidin-3-glycoside and cyanidin-
3-rutinocyte), the basic polyphenols of the fruit extract, are used in the treatment of eye defects and
eye diseases. In addition, blackcurrant extracts inhibit the development of cardiovascular diseases,
certain types of cancer, and chronic inflammation-related diseases. Quercetin derivatives found in
black currant leaves have a range of activities including antimicrobial, anti-inflammatory, antiviral,
antitoxic, antiseptic and antioxidant effects. Studies have shown that because the extracts from fruits,
buds and leaves of blackcurrant destroyed the free radicals by fighting them and increased body
resistance against many diseases, their use should be widespread and increased.

KEYWORDS
Blackcurrant, antioxidant, anthocyanin, vitamin C, health

Corresponding Author: HLYA VATANSEVER, vatanseverhulya@hotmail.com

1
AFYON KOCATEPE NVERSTES MHENDSLK FAKLTES GIDA MHENDSL BLM

1098
 Poster Session 8
Submission ID: 1031

A HERB WITH AROMATIC TASTE AND PLEASANT ODOUR:

COFFEE

1 2 1
ELIF EKMEK , MUHAMMED YUSUF ALAR , DUYGU ERCAN ORU

ABSTRACT
The coffee of the Rubiaceae family, first time seen in the Kaffa region of Abyssinia, it is mind
stimulant and one of the most popular beverages in the world with an aromatic taste. There are over 90
different types of coffee. Coffee; until phase of comsumption, it is passed from five stage such as the
maturation of the seeds, the collection, the separation of the crusts, the roasting and the grinding.
Coffee is a drink rich in antioxidants, phenolic and aroma components. The green coffee bean is rich
in antioxidants and phenolic compounds and contains high levels of chlorogenic acid. But green coffee
beans are very poor in flavor. Therefore, the green coffee beans are roasted at different times and
temperatures to produce complex prolytic reactions, thereby improving the taste and flavor of the
desired coffee. The most important factor in the aroma and flavor of the coffee is the degree of
roasting coffee beans. The roasted coffee has a very attractive and unique aroma. The most known
bioactive component of coffee is caffeine. Coffee, contains many chemical components such as
caffeine and chlorogenic acid. Due to these compounds, it has been stated that coffee have properties
such as improving mental and physical performance, increasing alertness. It was also found that the
chlorogenic acid in coffee is exhibited a high bioavailability and that 30% of the received chlorogenic
acid is excreted as metabolites within 24 hours. Studies have shown that some of these metabolites at
low M concentrations have neuroprotective properties. Studies conducted on decaffeinated, roasted
coffee have showed that coffee contain proadenosine, antimorphin and antioxidant compounds
exhibiting activity in brain. Studies, which were conducted in recent years, have been reported to show
a positive effect of coffee on diseases such as Parkinson's, Alzheimer's, etc.. Studies have shown that
coffee may prevent the development of Alzheimer's disease because picolinat detected in coffee
increases zinc absorption and L-rhamnose and fumarate in coffee show high antioxidant activity.
Decaffeinated coffee has been shown to reduce the risk of diabetes. Chlorogenic acids affect the
absorption and utilization of glucose. It has been also found that there are positive effects on liver
health and liver function. Various studies are needed in this regard.

KEYWORDS
Coffee, caffeine, chlorogenic acid, alzheimer

Corresponding Author: ELF EKMEK, elif_ekmekci@hotmail.com

1
AFYON KOCATEPE NVERSTES MHENDSLK FAKLTES GIDA MHENDSL BLM
2
STANBUL SABAHATTN ZAM NVERSTES, MHENDSLK VE DOA BLMLER FAKLTES, GIDA
MHENDSL BLM

1099
 Poster Session 8
Submission ID: 1032

THE EFFECTS ON HUMAN HEALTH OF KIWIFRUIT

1 1 1
ABDULLAH ALAR , ELIF EKMEK , HLYA VATANSEVER

ABSTRACT
There are a wide variety of species and forms of kiwifruit which are native to China. Among
these species, Actinidia deliciosa ('Hayward') and Actinidia chinensis ('Hort16A') are the most
significant two species with commercial importance. During fruit maturity, the fruit color of Actinidia
deliciosa is green while the fruit color of Actinidia chinensis is yellow. At the beginning of the 1900's,
the kiwifruit was not cultivated much outside of China, it was started to be grown in various countries
after these years. The promotion and adaptation studies of kiwifruit were made in 1988 in our country.
In 1994, our country entered agricultural statistics data. The largest share of the kiwifruit production
area in our country is the Black Sea Region. Kiwifruit; in adition to consumption as fruit, t is also
used in canned food, fruit juice, fruit yogurt, marmalade, jam, frozen or dried food, tea, cake sauces,
ice cream, pudding, wine, shampoo, soap and cosmetics. Kiwifruit is determined that it is a fruit rich
in phenolic compounds (anthocyanins and flavanoids), vitamins (vitamins C, B2, A and E),
antioxidant components, minerals (potassium, iron, phosphorus, calcium, chromium, copper and
magnesium) and carotenoids (xanthophyll, beta carotene and lutein). Kiwifruit; because of the high
nutritional content, high vitamin C content and low calorie levels, it is expressed as a health fruit. The
green kiwifruit fruit in 100 g fresh weight contains on average 85 mg ascorbate (vitamin C). Kiwifruit
has 3 times more vitamin C than orange. Like most fruits, although the kiwifruit contains water at high
concentrations, it contains vitamin E in higher concentrations than many other fruits. Vitamins C and
E, which are from antioxidants and are highly present in the contents of kiwifruit, prevent blockage of
arterial vessels. As a result of epidemiological, pharmacokinetic and metabolic studies, it has been
reported that the intake of vitamin C contributes to prevent certain cancers, cardiovascular diseases,
diseases related to upper respiratory tracts such as influenza and colds. Studies have determined that
kiwifruit improves intestinal and digestive health, alleviates constipation, strengthens immunity,
regulates lipid profile, improves iron uptake, improves DNA repair activity and reduces platelet
aggregation. The proteolytic enzyme actinidin, found in green kiwifruit, has been shown to help digest
food proteins, and kiwifruit consumption has been found to increase the number of appropriate
probiotic strains. In addition, recent studies have been determined that the sleep-inducing properties of
the kiwifruit are present. The most dense carotenoids found in the kiwifruit are -carotene and lutein,
which are considered as strong antioxidants. The lutein from the carotenoids is important in the
maintain of the vision. Kiwifruit has the highest lutein content among commonly consumed fruits.
Even though kiwifruit contains useful bioactive compounds, it contains some compounds such as
allergens and oxalate that may be harmful to health. The actinidin enzyme found in kiwifruit is allergic
to some people. The amount of oxalate present in the kiwifruit is not a concern for individuals who
sustain a balanced diet.

Corresponding Author: ELF EKMEK, elif_ekmekci@hotmail.com

1
AFYON KOCATEPE NVERSTES MHENDSLK FAKLTES GIDA MHENDSL BLM

1100
KEYWORDS
Kiwifruit, vitamin C, lutein, health

1101
 Poster Session 8
Submission ID: 1034

-AMYLASE AND -GLUCOSIDASE INHIBITORY ACTIVITIES OF

THE EXTRACTS AND CONSTITUENTS OF FERULAGO BRACTEATA
ROOTS

1 1 1 1 1
SONGL KARAKAYA , SEFA GZC , ZHAL GVENALP , HILAL ZBEK , HAFIZE YUCA , BENAN
1 2 1
DURSUNOLU , CAVIT KAZAZ , CEYDA SIBEL KILI

ABSTRACT
Context: Ferulago species have been used since ancient times for the treatment of intestinal
worms, hemorrhoids and as tonic, digestive, aphrodisiac and sedative. Apart from its medicinal uses,
they have been used as salad or spice due to their special odors. Objectives: This study reports -
amylase and -glucosidase inhibitory activities of extracts and bioactive compounds isolated from the
roots F. bracteata. Material and methods: The structures of isolated compounds through in vitro
bioassay-guided fractionation processes from the roots of F. bracteata were elucidated by detailed
analyses of 1D and 2D NMR and ESI-MS data. Results: A new coumarin, peucedanol-2-benzoate (1),
along with nine known ones, osthole (2), imperatorin (3), bergapten (4), prantschimgin (5),
grandivitinol (6), suberosin (7), xanthotoxin (8), felamidin (9), umbelliferone (10), and a sterol
mixture consisted of stigmasterol (11), -sitosterol (12) was isolated from the roots of F. bracteata.
Felamidin and suberosin showed significant -glucosidase inhibitory activity with 0.42 and 0.89
mg/mL IC50 values, respectively, when compared to the reference standard acarbose (IC50 4.95
mg/mL). On the other hand, none of the tested extracts were found to be active on -amylase
inhibition. Discussion and conclusion: The present study demonstrated that among the compounds
isolated from CH2Cl2 fraction of F. bracteata roots, coumarins were determined the main chemical
constituents of this fraction. This study aims to give first report on isolation and characterization of the
bioactive compounds from root extracts of F. bracteata and to report -amylase and -glucosidase
inhibitory activities of this species. Keywords: Ferulago bracteata; Apiaceae; -glucosidase; -
amylase; coumarin; peucedanol-2-benzoate.

KEYWORDS
Ferulago bracteata; Apiaceae; -glucosidase; -amylase; coumarin; peucedanol-2-benzoate.

Corresponding Author: SONGL KARAKAYA, ecz-songul@hotmail.com

1
ATATRK NVERSTES ECZACILIK FAKLTES
2
ATATRK NVERSTES FEN FAKLTES

1102
 Poster Session 8
Submission ID: 1037

ASSOCIATION OF PROBIOTIC AND PREBIOTICS WITH INSULIN

RESISTANCE, INTESTINAL PERMEABILITY AND ZONULIN
ASSOCIATED WITH POLICYSTIC OVER SYNDROME

1 1
ELIF ELK , GAMZE AKBULUT

ABSTRACT
Polycystic ovarian syndrome is the most common endocrine disorder of women in
reproductive age in the world. According to the National Institute of Health (NIH) prevalence is
reported as 6-10%, while prevalence according to Rotterdam criteria is reported as 15%. Insulin
resistance, obesity, dyslipidemia are complications associated with PCOS. In particular, insulin
resistance is one of the most important complications associated with PCOS. Many factors affecting
insulin resistance are addressed in PCOS and also intestinal permeability is one of these factors.
Disruption of the gut microbiota by various factors may lead to increased intestinal permeability,
which may lead to lower levels of inflammation and the development of chronic inflammatory
diseases. Increased intestinal permeability is associated with factors such as menstrual disorder,
obesity, insulin resistance, and dyslipidemia. Zonulin is a parameter that shows the change in intestinal
permeability. Intestinal permeability is associated with an increase in the level of zonulin. Individuals
with PCOS have increased levels of zonulin and are linked to insulin resistance. Probiotics and
prebiotics are shown in many studies in relation to obesity, insulin resistance and intestinal
permeability. They may be effective on PCOS-related parameters. A number of studies are carried out
on the effects of probiotics and prebiotics on insulin resistance, obesity and intestinal permeability, but
there are few studies directly affect on polycystic over syndrome . Probiotic supplementation on
women with PCOS significantly effect fasting plasma glucose, serum insulin concentration, insulin
resistance, -cell function, and serum triglyceride levels and may have beneficial effects on PCOS
semptoms . Similarly, probiotics effective on intestinal permeability and zonulin. In addition to
probiotics, prebiotics are affect gut microbiota, insulin resistance and intestinal permeability. In this
way, both prebiotics and probiotics may be effective on PCOS-related symptoms. However, increasing
the study done directly on individuals with PCOS will help to ensure that this relationship is shown.

KEYWORDS
polycystic over syndrome, insulin resistance, zonulin, probiotic, prebiotic

Corresponding Author: ELF ELK, elifcelik145@gmail.com

1
GAZ NVERSTES SALIK BLMLER FAKLTES

1103
 Poster Session 8
Submission ID: 1038

FATTY ACID COMPOSITION OF CHLORELLA VULGARIS GROWN

IN OPEN POND

1 1 1 2 1
YUSUF CAN GEREK , YIIT SABRI NL , EHSAN SARAYLOO , SALIM IMEK , HANDE MORGIL , MAHMUT
1 1
ALIKAN , GL CEVAHIR Z

ABSTRACT
Microalgal species, especially those grown in outdoor cultivation systems need a wide
tolerance to environmental conditions. Geographical location and climatic conditions, especially
temperature and solar radiation are the main environmental factors affecting lipid productivity
(Radolfi, 2009; Hindersin,2014). Generally, high added value compounds can be extracted from
microalgae, such as fatty acids (linolenic, arachidonic, eicosapentaenoic, docosahexaenoic acids, etc.),
pigments (carotenoids and ficobiliproteins), biochemically stable isotopes and vitamins such as biotin,
vitamins C and E; also some metabolites appear to have some pharmacological activities, among
others the anticholesterolemic, antitumoral, immunomodulatory, antibacterial and antimycotic ones
(Converti,2009). Chlorella has been the oldest commercial application of microalgae. Green algae
have the bulk of their fatty acids as saturated and unsaturated C18, a composition similar to that of
vegetable oils (Benemann and Oswald 1996). C. vulgaris, a fresh-water, fast-growing green alga, has
different lipid production capabilities (3040% of dry weight) under stress conditions. n this study,
Chlorella vulgaris were cultivated in 3-ton and 10-ton capacity open ponds with Bolds Basal medium
and added sodium bicarbonat for the additional CO2 source. Cells were harvested 6 weeks after
cultivation and dried in the oven at 65C. After that dried cells were powdered by using mortar. Total
lipid was extracted with n-hexane (60C) for 6 h using a Soxhlet extractor and FAMEs were prepared
using boron trifluoride in methanol (20% of BF3 in methanol) and extracted with n-hexane and then
analyzed by GC-MS.

KEYWORDS
Chlorella vulgaris, fatty acid, soxhlet

Corresponding Author: YUSUF CAN GEREK, yusuf.gercek@istanbul.edu.tr

1
STANBUL NVERSTES
2
KO NVERSTES

1104
 Poster Session 8
Submission ID: 1039

THE EFFECT OF GREEN COFFEE BIOACTIVE COMPONENTS ON

OBESITY

1 1 1 1
ELIF ELK , GAMZE AKBULUT , NILFER ACAR TEK , BRA ATABLEN

ABSTRACT
Obesity is a growing health problem worldwide. It is stated that nowadays about 600 million
adults and 41 million children are overweight and obese. Obesity Type 2 DM is associated with many
diseases, such as cardiovascular diseases, cancer, and non-alcoholic liver disease. There are many
factors affecting obesity such as genetics, increased energy intake, culture, and inadequate physical
activity. Depending on these factors, various treatments such as diet regimens, lifestyle modification,
physical activity, surgery are applied in the treatment of obesity. However, since each treatment
method has certain limitations, the use of functional foods in body weight control has become a
research topic. Coffee is also one of these foods and it is a complex structure composed of chemical
components. The chemical composition is influenced by the coffee bean species (Coffea arabica and
Coffea canephora), roasting and infusion. Chemical composition of green coffee beans before
roasting: 6.5-10% chlorogenic acid (CA), 1.2-2.2% caffeine, 10-16% lipids containing special
diterpene (Cafestol and kahweol), 0.7-1.0% trigonellin, 45-52% carbohydrates, 11% protein and 4.2-
4.4% mineral. The roasted coffee has a special aroma, taste and color due to the chemical reaction, and
a large number of positive bioactive components are formed during the roasting process. Chlorogenic
acids (CAs) are polyphenol compounds found in the seeds of Coffea arabica and Coffea canephora. In
general, the amount of commercial ground coffee varies depending on the roasting degree and the
percentage of Coffea arabica and Coffea canephora seeds in the mixture. Green coffee is a rich source
of chlorogenic acid derived from unroasted coffee beans and a source of caffeine. Chlorogenic acids
exhibit a variety of biological activities, including the ability to alter antioxidant enzyme activity, as
well as high antimutagenic, anticancerogenic, anti-inflammatory and antioxidant properties. In
addition to these features, there are both animal and human studies showing that body weight loss, fat
tissue hormone regulation, triglyceride levels, body fat percentage are also effective. Possible effects
on loss of body weight of green coffea, lipolytic effect on adipocytes, reduction of pancreatic lipase,
inhibition of fatty acid synthesis, increase of hydroxymethyl glutaryl CoA reductase and acetyl Coa
cholesterol acyl transferase, increase of AMP-active protein kinase-1 levels according to the results
obtained from studies And reducing acetyl CoA carboxylase activity. However, the bioavailability and
anti-obesity effect of coffea consumption with other foods, medicines and bioactive component
requires further research.

KEYWORDS
green coffee, obesity, body weight, chlorogenic acid

Corresponding Author: ELF ELK, elifcelik145@gmail.com

1
GAZ NVERSTES

1105
 Poster Session 8
Submission ID: 1040

OPTIMIZATION OF HERBAL TEA FORMULATION PRODUCED

FROM AROMATIC PLANTS

1 1 1 1
SERENAY AIK , TUE ATBAKAN , ZEHRA KASIMOLU , AYHAN TOPUZ

ABSTRACT
Tea (Camellia sinensis) is a specie from Theacea family grown in humid regions. Leaves and
buds of tea are used as beverage after extraction in hot water. Tea is one of the mostly consumed
beverage around the world due to its aromatic and refreshing properties. However, some people cannot
consume tea due caffeine sensitivity. Therefore, these people consume herbal beverages obtained from
different plants. Additionally, consumption of herbal tea increases progressively due to their health
beneficial and immune regulation effects. Different plants such as hibiscus, lemon peel, mint and
clove are used for production of herbal tea Hibiscus, caffeine-free plant, supports immune system due
to its high Vitamin C content. It decreases cardiovascular diseases risk, and regulates blood pressure.
Lemon peel inhibits free radicals and supports immune system. Mint has also a lot of benefits on
human health, especially on respiratory and gastrointestinal disorders. Clove is used for therapeutic
purposes on mouth and dental health. In addition, clove is good for tiredness and used for cold cure. In
the present study, hibiscus (Hibiscus sabdariffa), lemon peel (Citrus lemon), mint (Mentha spicata)
and clove (Syzygium aromaticum) were used for production of mixed herbal tea with high content of
phenolics and sensorial acceptability. A mixture design with 20 different experiments were created
using Design Expert software. Total phenolic content and sensory properties of the tea produced using
each mixtures were evaluated as response. Optimum herbal tea formulation were calculated as 0.380%
of hibiscus, 0.472 of lemon peel and 0.148% of clove . The optimum formulation was produced in
three replicates and total phenolic content and sensory properties (using hedonic scale) were
determined. taste, flavor and appearance point of optimum formulation were 6.07, 8.32 and 8.75,
respectively. In addition, total phenolic content of herbal tea was determined as 558 mg GAE/g dm.
Sensorial acceptability of optimum herbal tea produced in present research was found to be high.

KEYWORDS
Hibiscus, Clove, Mint, Lemon Peel, Mixed Herbal Tea, Total Phenolic Content

Corresponding Author: SERENAY AIK, serenayask@gmail.com

1
AKDENZ NVERSTES

1106
 Poster Session 8
Submission ID: 1041

FUNCTIONAL COMPONENTS OF RICE BRAN

1 2
HSEYIN BOZ , KAMIL EMRE GEREKASLAN

ABSTRACT

D
Rice (Oryza sativa) is one of the main grain crops and consumed by over half of the worlds
population, especially in Asia. It is emphasized that there is a relationship between white rice
consumption and many diseases such as cardiovascular and diabetes, especially in low-middle income

TE
countries. On the other hand, it is stated that the brown rice had many components, which has the
beneficial effect on human health, such as -aminobutyric acid, vitamins, phenolic substances, dietary
fiber, and -oryzanol. Although brown rice has the high nutritional composition and beneficial
physiological properties, brown rice consumption is very limited because of its undesired sensorial
EN
properties and poor cooking properties. The rice bran contains approximately 11-22% fat, 11-17%
protein, 6-14% fiber, 10-15% moisture and 8-17% ash. It is also particularly rich vitamins (especially
vitamin E, thiamine, niacin) and minerals (aluminum, calcium, chlorine, iron, magnesium, manganese,
phosphorus, sodium, potassium, zinc). Additionally, the rice bran also contains important nutraceutical
components such as tocopherols, tocotrienols, -oryzanol, ferulic acid, caffeic acid, tricin, coumaric
ES
acid. Because of this, the rice bran is one of the valuable by-products of the food industry. The aim of
this review, to give an information about the biologically active compounds of rice bran and their
beneficial effects on human health.
PR

KEYWORDS
Rice bran, nutraceutics, -oryzanol
T
O
N

Corresponding Author: KAML EMRE GEREKASLAN, emre@nevsehir.edu.tr

1
ATATRK UNV., TOURSM FAC., GASTRONOMY AND CULNARY ARTS DEPT., 25240 ERZURUM, TURKEY.
2
NEVEHR HACI BEKTA VEL UNV., ENGNEERNG-ARCHTECTURE FAC., FOOD ENGNERNG DEPT.,
50300 NEVEHR, TURKEY.

1107
 Poster Session 8
Submission ID: 1043

AS A PROTECTIVE AND THERAPEUTIC AGENT AGAINST

ALZHEIMERS: CURCUMIN

1 1 1 1
BRA ATABLEN , GAMZE AKBULUT , NILFER ACAR TEK , HILAL BETL ALTINTA

ABSTRACT
Alzheimer's is a progressive and irreversible neurodegenerative disease that affects 27 million
people worldwide. Alzheimer's may be developed according as many pathophysiological conditions.
Studies that have been done over the last two decades are to understand underlying causes of
Alzheimer's and to develop new protective and therapeutic methods. For this reason, recent studies
have focused on spices that have flavor, coloring or protective properties as well as effects of reducing
the risk of chronic diseases. In particular curcumin, a component of turmeric, is being investigated for
its use in the treatment of Alzheimer's. In 2000, Ganguli et al. have reported a lower prevalence of
Alzheimer's in India where people consume curcumin as part of the curry spice compared to the
United States or Ng et al. have indicated that cognitive performance was better in elderly individuals
who often consume curry. These studies pioneered in vivo and in vitro studies in order to investigate
the protective effects of curcumin on alzheimer's. For example, in a small study in Japan, 3 patients
with Alzheimer's were treated with 100 mg/day of curcumin for 12 weeks. At the end of the study, the
mini-mental status assessment scale score was significantly increased in only one of the patients.
However, the patients began to remember their families within a year. Also in studies performed in
different mouse models, curcumin supplementation at different doses decreased -amyloid plaque
formation, reactive oxygen species and proinflammatory factors. At the same time, an increase in
cognitive performance after treatment was observed. Several mechanisms related to the protective and
therapeutic properties of curcumin on alzheimer have been proposed. These: Curcumin is a powerful
antioxidant. With this feature, it protects against protein oxidation. It is reported that it suppresses
early growth response-1 (Egr-1) activation due to its anti-inflammatory effect. This may represent a
potential therapeutic approach for Alzheimer's. It has the ability to bind iron, copper and zinc
effectively. This inhibits nuclear factor kappa (NF-k) induction and plays a protective role against
metal-induced neurotoxicity. Because it has the ability to cross the blood-brain barrier, it binds
directly to small -amyloid oligomers, prevents -amyloid accumulation and toxicity. Curcumin
reduces hypercholesterolemia, which can play an active role in alzheimer's development, through
upregulation of apolipoprotein A1, lecithin-cholesterol acyltransferase and low-density lipoprotein
(LDL) -receptor genes. It stimulates embryonic neuronal cell proliferation and hippocampal
neurogenesis. Curcumin, promotes neuronal protective effect in the brain by increasing expression of
heat shock protein (Hsp). As a result, curcumin has the potential to be a protective and therapeutic
agent for Alzheimer's. Although not in sufficient numbers, in most of the studies have been reported
that curcumin have beneficial effects on Alzheimer's. It has also been recently tested for its utility as a
diagnostic tool for Alzheimer's because of its natural fluorescence and affinity for binding to -
amyloid protein. In addition to the positive properties, low bioavailability of oral curcumin restricts its
protective or therapeutic role. To increase curcumins bioavailability, addition of components such as

Corresponding Author: BRA ATABLEN, busra.atbln@hotmail.com

1
GAZ NVERSTES SALIK BLMLER FAKLTES BESLENME VE DYETETK BLM

1108
piperine to the formulations may interact with the drugs. Alternatively, the use of curcumin at high
doses to achieve the desired effect may stimulate an increase in reactive oxygen species. For this
reason, the acceptable intake quantity determined by The Joint FAO (Food and Agriculture
Organization) and WHO (World Health Organization) Expert Committee on Food Additives (JECFA)
should not exceed 0-3 mg/kg/day.

KEYWORDS
curcumin, alzheimers, protective and therapeutic

1109
 Poster Session 8
Submission ID: 1044

TOTAL PHENOLIC CONTENT AND SENSORY PROPERTIES OF

MIXED HERBAL TEA PRODUCED FROM DIFFERENT AROMATIC
PLANTS

1 1 1 1
TUE ATBAKAN , SERENAY AIK , ZEHRA KASIMOLU , AYHAN TOPUZ

ABSTRACT
Tea is a beverage produced by boiling or infusing of tea (Camellia sinensis) leaves. Tea is the
mostly consumed beverage in the world after water. Herbal teas are also consumed frequently due to
its aromatic properties and beneficial health effects. Herbal Tea can be described as beverages
obtained from processed fruit or plants. Those are also prepared in hot water via decoction or
infusion. Demand of aromatic plants such as ginger, licorice, cinnamon and cardamom for production
of herbal tea increases day by day due to their beneficial effects on human health. Ginger is an
aromatic plant which is rich in carbohydrates, essential oils, Vitamin A and C. It is frequently used for
treatment of stomach disorders. Additionally, it is good for throat ache and helpful for detoxication.
Similarly, licorice is used in treatment of throat ache and cough for many years. Cinnamon is used for
treatment diabetes mellitus due to its reducing effect on blood glucose level. It is also effective on the
sniffles, cold and flu. Although cardamom is not known adequately in Turkey, it has a lot of beneficial
effects on human health. Especially, it is used against digestion problems deodorization of bad breath.
In addition, it is an appetizing plant and can be used for failure to thrive problems. In the present
study, to produce a mixed herbal tea with high phenolic content and sensorial acceptability, ginger
(Zingiber officinale), licorice (Glycyrrhiza glabra), cinnamon (Cinnamomum verum) and cardamom
(Eletteria serrula) were used. A mixture design including 20 different herbal tea mix was created using
Design Expert program. Total phenolic content and sensory analysis results were used as response of
the mixture design. According to optimization results, 0.072%, 0.765% and 0.163% of licorice,
cinnamon and cardamom were determined as optimum formulation, respectively. Total phenolic
content and sensory analysis (with 9 points hedonic scale) were carried out for mixed herbal tea
produced at optimum conditions. Sensory properties of taste, flavor and appearance were determined
as 6.75, 7.69 and 7.44, respectively. Total phenolic content of the optimized formulation was
determined as 369.95 mg GAE/g dm. The experimental values of the sensorial properties and total
phenolic content of optimum formulation were in agreement with theoretical values. Herbal tea
formulation determined in the present study was highly acceptable by consumers.

KEYWORDS
Licorice, Cinnamon, Ginger, Cardamom, Mixed Herbal Tea, Total Phenolic Content

Corresponding Author: TUE ATBAKAN, tugceatbakan@gmail.com

1
AKDENZ NVERSTES

1110
 Poster Session 8
Submission ID: 1045

LAUREL REHABILITATION

1
OZAN ACUN

ABSTRACT
Any kind of vegetable (fruit, seed, flower, leaf, bark, root, shoot, onion, tuber, rhizome,
mushroom etc.) that grow in the forests and openings and that people and other living beings use to
supply their own needs or to provide income. (FOCUS). Can be defined as Non-Wood Forest Products
(ODO). Can be defined as Non-Wood Forest Products (ODO). The main Asian homeland is in
Asia Minor and in the Balkans, all green is a vegetation type. It is one of the characteristic plants of
the Mediterranean and the Mediterranean is called the Lauretum zone of the Mediterranean region.
Bay, one of the 40 genera of the Laureceae family, grows naturally in the coastal waters of the
Aegean, Mediterranean and Black Sea regions of our country. The leaves are in an elliptical structure
and are tough or rough like the skin. They are 5-10 cm in length and 2-4 cm in width. The edges of
leaves are slightly wavy. 'The number of shoots in the January is the leaf yield and the blockages of
the shoots are made and animation sections are made and it is tried to determine how many shoots
should be left in the shoot dilution process.The defoliation can be seen in almost all different
environments of growth (wet creek, rocky rocky areas) within the area. Detections were made
according to the Braun-Blanquet scale. After the necessary examinations, determinations and sample
procurement were completed, firstly the cover against the area was cut off and lastly the animation
section was applied on the treasure hobs. 2013 and 2015 were cleaned twice in the area. At the end of
the project, deflaze leaf was produced and weighed in full area, in quarries. In the analysis of the data;
Variance Analysis, Duncan Test and Kruskal-Wallis Test were used. As a result of the statistics, it is
evaluated that the dropout process in the first group and the first one, which is located in the first rank,
can be implemented.

KEYWORDS
Laurel Rehabilitation

Corresponding Author: OZAN ACUN, ozanacun@ogm.gov.tr

1
ZONGULDAK ORMAN BLGE MDRL

1111
 Poster Session 8
Submission ID: 1046

SUPERCRITICAL CARBON DIOXIDE EXTRACTION OF SEA

BUCKTHORN (HIPPOPHAE RHAMNOIDES L.) SEED AND FATTY
ACID COMPOSITION

1 1 2 1 1
ALIYE ARAS PERK , YUSUF CAN GEREK , FATMA DUYGU CEYLAN , HEYDA GLEN , ONUR YANAR ,
1 2
MEVZULE YAZGAN , ESRA APANOLU

ABSTRACT
Sea buckthorn (Hippophae rhamnoides L.) is a bush that grows wildly in the mountain regions
of the middle and southeastern Asia and Europe. Its fruit are berries of orange to red colour and have
an acid, lightly bitter taste. They contain many vitamins (B, C, E, K, provitamin A) and other
biologically active substances. The main products obtained from the fruit are juice rich in vitamin C
and oil rich in unsaturated fatty acids. Seabuckthorn berry oil having high nutraceutical,
cosmeceutical, and therapeutic activity has been extracted from dried seabuckthorn (SBT) whole berry
powder using supercritical carbon dioxide (SC-CO2), a green process for extraction of bioactives.
Wild Turkey berries of Hippophae rhamnoides L. were collected from twenty-one different locations.
Seeds were isolated from berries. For each experiment, dried SBT berry seed was subjected to SC-
CO2 extraction. Fatty acid methyl esters in the sea buckthorn extract were determined by GCMS.
The dominating fatty acids in seed oils were oleic (18-33 %), linoleic (11-32%), alfa linoleic (9-21%).
The seed of sea buckthorn berries are rich in lipid which is rare in the plant kingdom. The studied
samples of sea buckthorn from Turkey have proven to be potential sources of valuable oils.

KEYWORDS
Sea buckthorn, Hippophae rhamnoides L., Fatty acids, Supercritical carbondioxide extraction

Corresponding Author: YUSUF CAN GEREK, yusuf.gercek@istanbul.edu.tr

1
STANBUL NVERSTES
2
STANBUL TEKNK NVERSTES

1112
 Poster Session 8
Submission ID: 1047

THE IMPORTANCE OF CHIA (SALVIA HISPANICA L.) PLANT AND

POSSIBILITIES OF CULTIVATION

1 1 1
UUR TAN , OLCAY ARABACI , HATICE KBRA GREN

ABSTRACT
Salvia hispanica L. is a flowering plant that has edible seeds of the Labiatae family and known
as chia. Salvia hispanica L. seeds are a traditional food of Central and South America. The 16th
Century Codex Mendoza records indicate that the plant was cultured in pre-Columbian times and
those seeds were used at that time. In addition that It has been reported that seed was used as a body
moisturizer (medicine), varnishing of pottery etc. (oil) and edible in the form of mushes roasting or
without roasting Chia seeds contain 15-25% protein, 30-33% fat (alpha-linolenic acid) (ALA), 26-41%
carbohydrates, 18-30% fiber, 4-5% ash, 90-93% dry matter. Additionally, it contains high antioxidant.
In recent years, heart and vascular diseases, diabetes and obesity have increased in the worldwide and
in Turkey year after year. Malnutrition plays a major role in the occurrence of these conditions. People
who have come to realize that have searched for healthier foods (Novel Food) than those traditionally
ones for health. The interest on this plant has been increased due to conducted studies about Chia
(Salvia hispanica L.) that proof that valuable resource for human health and nutrition. For that reason,
it is necessary to include Salvia hispanica in the cultivation and breeding programs and produce it like
other traditional field crops. Thus, new, nutritious, healthy and healing alternative food source that
people can edible will be offered. In this article, importance and possibility of cultivation of chia plant
were investigated.

KEYWORDS
Salvia hispanica L., Chia, nutritional value, breeding, cultivation.

Corresponding Author: HATCE KBRA GREN, hkubra.goren@adu.edu.tr

1
ADNAN MENDERES NVERSTES, ZRAAT FAKLTES

1113
 Poster Session 8
Submission ID: 1048

EFFECTS OF PROBIOTICS ON MENTAL HEALTH

1 1 1 1 1
BRA ATABLEN , GAMZE AKBULUT , NILFER ACAR TEK , ELIF ELK , YELIZ SERN

ABSTRACT
Between the gastrointestinal system and the central nervous system, there is a two-sided and
functional relationship involving anatomic connections such as the vagus nerve, the immune system,
the hypothalamus-pituitary-adrenal axis (HPA axis). The other key component involved in this
relationship is the intestinal microbiota. It has recently been thought that the microbiota-intestine-brain
axis plays an important role in the neurodevelopmental phase. Therefore, microbial changes are
associated with the development of diseases such as inflammatory bowel disease, obesity as well as
neurodegenerative diseases such as anxiety, depression, alzheimer's, parkinsonism and autism. The
increasing prevalence of these diseases, which affect mental health negatively, has accelerated the
studies that study microbiota and the healing effect of probiotics on microbiota. Although studies on
humans are still inadequate, the data obtained from these studies show that probiotics have therapeutic
effects on depression and anxiety. It was also observed that probiotic supplementation in individuals
with Alzheimer's disease significantly improved their mini-mental status assessment score. In addition
to studies on humans, studies are also carried out in different animal models. According to the results
of these studies, it has been found that supplements containing different probiotic species reduced
anxiety and depression findings in animal models. Because each of the probiotic bacteria has different
feature, many mechanisms have been proposed for the positive effects of probiotics on mental health:
Mental health-related diseases are associated with factors such as inflammation, oxidative stress, and
increased release of inflammatory cytokines. Probiotics control glutathione levels by increasing
glutamate-cysteine ligase activity. Thus, affecting the local and systemic antioxidant status; probiotics
positively influence mental health. Lactobacillus and bifidobacteria species on the gastrointestinal
tract are important in the synthesis of gamma-amino butyric acid (GABA). Gamma-aminobutyric acid
is an inhibitor-specific neurotransmitter that increases release in neuropsychiatric conditions such as
anxiety and depression. At the same time, acetylcholine neurotransmitter, which is important in
cognitive events such as memory, concentration is synthesized by subspecies of lactobacillus.
Probiotics can affect mental health positively by providing the proliferation of beneficial bacteria that
play a role in the synthesis of GABA and acetylcholine. Approximately 90% of serotonin is
expressed in enterochromaffin cells in the gastrointestinal tract. For this reason, intestinal microbiota,
which controls the production of serotonin, can directly affect the functions of the central nervous
system. Or the probiotics can increase the plasma level of tryptophan, the precursor of serotonin. The
kynurenic acid resulting from the metabolism of the tryptophan has neuroprotective effect. Probiotics
regulate central nervous system functions by acting on intestinal barrier permeability. Because
intestinal barrier permeability is essential for maintaining of the immunity and the nervous system.
Increased permeability of insteinal barrier has been associated with diseases such as depression and
autism. Reducing corticosteroid response, it alleviates the HPA stress responses having an impact on
emotional state and mood. Improving carbohydrate malabsorption positively, probiotics can affect

Corresponding Author: BRA ATABLEN, busra.atbln@hotmail.com

1
GAZ NVERSTES SALIK BLMLER FAKLTES BESLENME VE DYETETK BLM

1114
mental health. As a result, changes in intestinal microbiota may cause changes in the central nervous
system. This information may provide to development of new strategies in the use of probiotics as an
adjunct for development of cognitive and sensory aspects of mental health. At the same time,
probiotics may enhance the efficacy of psychopharmacology because they influence expression of
genes in the brain. This makes possible the use of pharmacological active ingredients at low doses and
reducing the toxic side effects of the components.

KEYWORDS
probiotics, mental health, intestinal microbiota

1115
 Poster Session 8
Submission ID: 1049

LIQUORICE AS A FUNCTIONAL FOOD

1 1 1
MUSA SERDAR AKIN , MUTLU BUKET AKIN , BRA GNC

ABSTRACT
Liquorice, or licorice, is the root of Glycyrrhiza glabra from which a sweet flavour can be
extracted. The liquorice plant is a herbaceous perennial legume native to southern Europe and parts of
Asia, such as India. The liquorice plant grows widely in our country especially in South, Central and
Eastern Anatolia. It is not botanically related to anise, star anise, or fennel, which are sources of
similar flavouring compounds. The composition of liquorice are gum-starch (26-42%), moisture (13-
17%), ash (6-9%), sucrose (6-12%), invert sugars (6-9%), glycyrrhizin ) and resin (4-8%). The scent
of liquorice root comes from a complex and variable combination of compounds, of which anethole is
up to 3% of total volatiles. Much of the sweetness in liquorice comes from glycyrrhizin, which has a
sweet taste, 3050 times the sweetness of sugar. The sweetness is very different from sugar, being less
instant, tart, and lasting longer. Liquorice is used as a flavouring agent for tobacco, candies,
sweeteners, yogurt or ice cream. It is used as a sweet, mouth freshener or drink many countries, such
as Netherlands, Italy, Spain, France, Syria, Egypt and Turkey.In yorkshire ponferact cakes originally
made from liquoris. Liquorice is used by brewers to flavour and colour porter classes of beers. In
Calabria/Italy a popular liqueur is made from pure liquorice extract. Liquorice extracts have a number
of medical uses, and they are also used in herbal and folk medications. The isoflavene glabrene and the
isoflavane glabridin, found in the roots of liquorice, are phytoestrogens. The compound glycyrrhizin
(or glycyrrhizic acid), has also demonstrated antiviral, antimicrobial, anti-inflammatory,
hepatoprotective, and blood pressure-increasing effects in vitro and in vivo, as is supported by the
finding that intravenous glycyrrhizin (as if it is given orally very little of the original drug makes it
into circulation) slows the progression of viral and autoimmune hepatitis. In one clinical trial liquorice
demonstrated promising activity, when applied topically, against atopic dermatitis. Additionally,
liquorice may be effective in treating hyperlipidaemia (a high amount of fats in the blood). Liquorice
has also demonstrated efficacy in treating inflammation-induced skin hyperpigmentation. Liquorice
may also be useful in preventing neurodegenerative disorders and dental caries. The antiulcer,
laxative, antidiabetic, anti-inflammatory, immunomodulatory, antitumour and expectorant properties
of liquorice have been investigated.Glycyrrhizin has been proposed as being useful for liver protection
in tuberculosis therapy, but evidence does not support this use, which may in fact be harmful.
Excessive consumption of liquorice (more than 2mg/kg/day of pure glycyrrhizinic acid, a liquorice
component) may result in adverse effects, and overconsumption should be suspected clinically in
patients presenting with otherwise unexplained hypokalemia and muscle weakness.

KEYWORDS
Liquorice, composition, functional food, medical uses

Corresponding Author: MUSA SERDAR AKIN, sakin@harran.edu.tr

1
HARRAN NVERSTES

1116
 Poster Session 8
Submission ID: 1050

MEDICINAL PLANT POTENTIAL OF BALIKESIR

1 1 1
FATIH SATIL , SELAMI SELVI , GLENDAM TMEN

ABSTRACT
This study carried out between 2014 and 2016 was done to identify the medicinal plants
spreading at flora of Balkesir and to reveal the medicinal and aromatic plant potential of Balkesir.
For this purpose, herbariums of plant specimens collected periodically in different regions of Balkesir
were done. In the determination of medicinal plants; both Commission E, Pharmacopoeia and various
Monographs were used, as well as species used local people with field studies were identified. As a
result of the research; 144 genera and 179 taxa belonging to 61 families could be seen included in the
potential medicinal plant class. The families to be the highest number of taxa are; Lamiaceae (33 taxa),
Asteraceae (21 taxa) and Rosaceae (15 taxa), respectively. Also, 59 taxa are registered in Commission
E and various Pharmacopoeia with Monographs. According to the results obtained in the study, it was
seen that the plants were mostly used with the infusion method by local people. With this study, it is
seen that Balkesir has a quite rich medicinal plant potency and of this wealth could be seen
contributed to the pharmaceutical sector by making a significant contribution to the country's
economy.

KEYWORDS
Medicinal plants, Flora, Medicine, Balkesir

Corresponding Author: FATH SATIL, fsatil@gmail.com

1
BALIKESR NVERSTES

1117
 Poster Session 8
Submission ID: 1051

HAVE ECONOMIC POTENTIALS WOODEN OUTDOOR PRODUCTS

INTRODUCTION GUIDE

1
OZAN ACUN

ABSTRACT
Our country has diversity of genetic, flora, fauna and ecosystem to show continental character.
Approximately 3,000 of these are endemic to my country, with a plant diversity expressed in
approximately 12,000 taxa. Our forests are not only rich in biodiversity but also rich in wood products.
It can not be said that the forestry sector has benefited from the non-wood forest products sufficiently.
The purpose of preparing this work is; Identify the value of the country's economy by identifying the
presentations, production, processing and marketing of non-wood forest products and the solutions to
them. By applying this work to practice; The result of employment of forestry engineers, forestry
industry engineers and similar technical personnel in the process of processing and improving the
forest village, reducing the pressure on the forest, production of non-wood forest products and
procurement, marketing and consumption of raw materials . In addition to creating added value to the
country's economy, it may also be possible to provide foreign currency entry by exporting. With this
book, the promotion and promotion of non-wood forest products which are not explained to this day is
done. This work also has the feature of becoming a source in our educational times. Local people in
Zonguldak, Bartn and Karabk earn these economies by collecting and selling these products which
are grown at the end. Preliminary evaluations made are not as many as the number of people in this
person. Losses of detected products. These provide the wrong collection, drying and storage
conditions. The most important condition of a sustainable use is not to damage the resources. During
this time we have to evaluate the eye contact in his time. The purpose of this guide is to introduce
better forest products without Zonguldak, Bartn and Karabk. Sustainable production is essential.

KEYWORDS
HAVE ECONOMIC POTENTIALS WOODEN OUTDOOR PRODUCTS INTRODUCTION
GUIDE

Corresponding Author: OZAN ACUN, ozanacun@ogm.gov.tr

1
ZONGULDAK ORMAN BLGE MDRL

1118
 Poster Session 8
Submission ID: 1052

ASSESSMENT OF ANTIMICROBIAL ACTIVITY OF BLACKTHORN

(PRUNUS SPINOSA L.) EXTRACTS ON SOME SELECTED
MICROORGANISMS

1 1
MUHAMMED ZEKI DURAK , GULSUM UCAK , EZGI METN

D
ABSTRACT

TE
Blackthorn (Prunus spinosa L.) generally grows wild in the Thrace region of Turkey. It is a
deciduous large shrub or a small tree growing up to 5 meters in height. The extracts of fresh
blackthorn contains high amount of polyphenolic compounds which can suppress the negative effect
of free radicals in the organism. For this reason, they possess health benefits such as free radical

EN
scavenging, antioxidant, anti-inflammatory, antimicrobial, and anticancer activity. The aim of this
study was to evaluate the antimicrobial action of aqueous extracts from Prunus spinosa L. fresh fruit
on nine bacterial strains (B. subtilis ATCC 6633, E.coli ATCC 25922, P.aeruginosa ATCC 27853,
Salmonella Typhimurium ATCC, L. monocytogenes ATCC 13932, Klebsiella pneumoniae ATCC
43816, Bacillus cereus ATCC 11778, E.coli O157:H7 ATCC 43888 and S. aureus ATCC 25923) and
ES
two fungal strains (S. cerevisiae ATCC 9763 and Candida albicans ATCC 10251). Blackthorn fruit
was obtained from anakkale, Turkey. The stone of fruit was removed and the remaining material was
grounded via a blender. Twenty (20) gram of sample was weighed and extracted with 200 mL of %75
ethanol at room temperature with shaker for 2 h. After filtering and centrifuging the resulting aqueous
solution, the supernatant was evaporated at 50 C in a rotary evaporator. The concentrated solution
PR

was dried at 50 C for overnight in an oven and the initial concentration was designated with sterile
distillated water as 1g/mL. Antimicrobial tests were carried out by disc diffusion method in which
antibacterial activity was evaluated by measuring the zone of inhibition against test microbial strains.
Ethanol fruit extract showed antimicrobial activity against all tested bacteria. The antifungal activity
was tested against two organisms S. cerevisiae ATCC 9763 and Candida albicans ATCC 10251. The
T

investigated extract exhibits antifungal activity against S. cerevisiae ATCC 9763. S. cerevisiae ATCC
was more susceptible to the extracts than all tested microorganisms. L. monocytogenes ATCC 13932
O

was showed the highest antimicrobial activity among the others pathogen bacteria. These results
showed that Candida albicans ATCC 10251 was found the most resistant among the microorganisms
tested against blackthorn extract, followed by Klebsiella pneumoniae ATCC 43816. A long as the
N

concentration of the extract decreased, the level of antibacterial activity and the size of inhibition zone
alleviated directly. In general, the inhibition zones of microorganisms were ranged from 8.00 mm to
31.75 mm. On the basis of obtained results, we concluded that the investigated blackthorn (Prunus
spinosa L.) fruit extract have significant antimicrobial activity.

KEYWORDS
Prunus spinosa L., Antimicrobial Activity, Disk Diffusion Method,

Corresponding Author: MUHAMMED ZEK DURAK, mzd2@cornell.edu

1
YLDZ TECHNCAL UNVERSTY

1119
 Poster Session 8
Submission ID: 1053

EFFECTS OF PHOENIX DACTYLIFERA ON MALE INFERTILITY

1 2
MUSA TATAR , TUBA TATAR

ABSTRACT
Infertility is an important health problem which affects one in six couples. Male-originated
factors cause infertility approximately 60%. Many factors such as physical activity, environmental
pollution, using of certain drugs or alcohol and tobacco, and nutrition influence reproductive
functions. Among these factors, the effects of nutrition on reproductive functions have been
particularly noted in recent years. Consumption of some foods can cause sperm quality to increase or
decrease in men. For example; decrease in the consumption of vegetables and fruits reduces the intake
of antioxidants. Therefore sperm quality is affected negatively. Herbal antioxidants protect the sperm
from oxidative stress and help to survive. The date palm (phoenix dactylifera) which has been grown
in Southwest Asia and Northern Africa, has a high antioxidant composition. Composition of the date
palm contains approximately 3942 mg/100 g of carotenoid and phenolic compounds and 80400
g/100 g of antioxidant compounds. In studies, the administrations of date palm products with
different doses cause an increase in FSH, LH, testosterone and estradiol hormone levels, a significant
increase in weights of the testicle and epididymis of rats and have positive effects on spermatogenesis
and sperm parameters such as an increase in sperm count and motility. It is observed an increase in
Leydig cells which produce testosterone, sperm concentration in seminiferous tubules and a decrease
in testicular DNA damage and adverse effect created by infertility agent on spermatogenesis. There is
an association between sex hormones and sperm parameters. FSH hormone facilitates spermatogenesis
in seminferous tubules via binding sertoli cells. Decrease in testosterone levels causes lower sperm
concentrations in the epididymis. Estrogen regulates reabsorption of the luminal fluid on head of
epididymis that leads more concentrated sperms. Increase in sex hormones is an indicator for increase
in secretory activity that causes an increase in weights of the testicles and epididymis. In conclusion,
antioxidant factors within composition of the date palm have an efficient role on spermatogenic cells
and may be useful to prevent and treat infertility.

KEYWORDS
Date Palm, Phoenix Dactylifera, Infertility

Corresponding Author: MUSA TATAR, musatatar88@hotmail.com

1
MEHMET AKF ERSOY NVERSTES
2
GAZ NVERSTES

1120
 Poster Session 8
Submission ID: 1054

AMASYA REGIONAL DIRECTORATE OF FORESTRY RESEARCH

ON POTENTIAL OF RUSCUS ACULEATUS

1 1
ZEKERIYYA NANE , MUTLU TURAN

ABSTRACT
There are significant deficiencies in the production studies of non-wood forest products work
in our country's forestry. Problems arise about continuous and planned operation of non-wood forest
products that meet the raw material demands of many industries such as food, medicine, cosmetics.
One of the most important reasons of the problems is that the inventory studies of non-wood forest
products have not been done sufficiently. Within this scope, inventory studies of non-wood forest
product were started in 2013, Amasya Regional Directorate of Forestry. It is aimed that planned
production and marketing with inventory of Ruscus aculeatus used in alternative medicine and
pharmaceutical industry. In this study, the distribution areas, growth environment characteristics,
production and marketing issues of the Ruscus aculeatus L which is naturally found in the Amasya
Regional Directorate of Forestry, Bafra Forest Management Directorate, Ondokuzmays Enterprise
will be revealed and application results will be evaluated.

KEYWORDS
Amasya Regional Directorate of Forestry, Ruscus aculeatus L.

Corresponding Author: MUTLU TURAN, mutluturan@ogm.gov.tr

1
AMASYA ORMAN BLGE MDRL

1121
 Poster Session 8
Submission ID: 1055

THE USE OF BETALAINS AS NATURAL COLOR MATERIALS AND

THEIR EFFECTS ON HEALTH

1
HSEYIN GENCELEP

D
ABSTRACT
THE USE OF BETALAINS AS NATURAL COLOR MATERIALS AND THEIR EFFECTS
ON HEALTH Hseyin GENCELEP Ondokuz Mays University, Faculty of Engineering,

TE
Department of Food Engineering Samsun Betalaines are structurally water-soluble nitrogenous
pigments and contains a derivative of nitrogen, betalamic acid. Red beet is the only source approved
and approved for use in food and pharmaceutical products for betalain coloring matter. Betalamic acid
forms different structures as a result of biosynthesis with various molecules. These structures are
EN
generally called betaxanthin and betacyanin. Betaxanthin is yellow color pigment and betasiyanin are
red-violet pigments. In the investigations conducted, about 50-70 betalaine were detected. 50% of
these are betasiyanin; and 20% is the betaxanthins. It is known that many factors influence the stability
of betalaines. The degree of glycosylation and acylation in the high affects positively the low water
ES
activity, the presence of antioxidant, the pH 3-7 range, the pigment content of the plant in the low
temperature and dense nitrogen environment. On the other hand, it is suggested that temperature, light,
oxygen, high water activity, low degree of glycosylation, low acylation and existence of metal ions
negatively affects. The optimum pH for the enzymatic degradation of both betacillins and betaxanthins
has been reported to be about 3.4. Red beet is quite rich in color pigment called betalaine. This color
PR

can be used as a coloring agent in food and pharmaceutical products by making pigment into a
powder. Betalins have a wide range of biological activities including antioxidants, antiinflammatory,
hepato-protective and anti-cancer properties. Antioxidant properties of betalaines and phenolic acids
have been reported to prevent age-related diseases, cancer and cardiovascular diseases. It is also
reported that antiviral and antimicrobial effects are present. The antioxidant betalaines found in the red
beet are betanein and betanidine 5-O--glucoside. It has also been determined that betalaine in red beet
T

showed potent health benefits such as strengthening the immune system and preventing cardiovascular
diseases, neurodegenerative disorders and cancer, as well as free antioxidant and free radical
O

scavenging properties. The fact that it is a good electron donor thanks to a phenolic group and a cyclic
amine group implies the antioxidant property. Among the betalain components in the beet, attention
N

has been drawn to recent studies that betacyanins are usually one of the most important anti-cancer
compounds. Betaine, one of the most important components of betalain, has been identified as the
primary and potent compound responsible for anti-cancer effects. Betaine, isolated from red beets, has
been shown to exhibit a dose-dependent growth inhibition on human chest, lung, colon, abdominal and
central nervous system tumors. In conclusion, studies on the health effects of red beet pigment
betalaine and studies on the anticancer effects are at an early stage and have been used in combination
with strong anticancer drugs currently used to provide synergistic effects with novel studies and
particularly effective dose reduction potency and reduce drug toxicity related to treatment research is
needed.

Corresponding Author: HSEYN GENCELEP, genccelep@hotmail.com

1
ONDOKUZ MAYIS NVERSTES

1122
KEYWORDS
Natural coloring material, betalaine, betaine

1123
 Poster Session 8
Submission ID: 1056

EDIBLE WILD MUSHROOMS AS FUNCTIONAL FOODS

1
HSEYIN GENCELEP

ABSTRACT

D
EDIBLE WILD MUSHROOMS AS FUNCTIONAL FOODS Hseyin GENCELEP
Ondokuz Mays University, Faculty of Engineering, Department of Food Engineering Samsun
Functional food "defines foods that provide a health benefit as well as stimulating nutrients. Another

TE
definition; food or food ingredients that provide additional benefits on human physiology and
metabolic functions beyond the basic nutritional needs of the body so that they are effective in
protecting from disease and achieving a healthier life. In addition to nutritional effects, one or more
active ingredients have the effect of reducing the risk of disease-protective, corrective and /or disease
EN
risk, these effects being called scientific and clinically proven food functional foods. For centuries,
macrofungi, a good source of food for human beings, as well as high protein and vitamin content, It is
rich in fiber, carbohydrates and minerals and is a valuable food with a low fat content. Fungi are
separated from other vegetables because they have easy digestible proteins. Important amino acids in
the composition of edible fungi are vitamins B (thiamin, riboflavin, nicotinic acid, biotin) and vitamins
ES
C, D and K. Cooked or edible fungi processed by various methods are a good dietary component for
vegetarians because of their superiority in nutrition. It is also suitable for the consumption of diabetics
and heart patients. Since it is rich in folic acid, mushrooms are used to treat anemia. In addition,
macrofungi are rich in minerals such as calcium, phosphorus, potassium, iron and copper. The dry
PR

matter content of fresh mushrooms is relatively low, ie around 10%, and predominantly consists of
carbohydrate, protein, fiber and minerals. The composition of the mushroom contains 90.7% water,
3.5% protein, 0.3% fat, 4.5% carbohydrate and 1% mineral matter. In the production of ready-made
soup and pizza raw materials, various sauces and baby foods, dried mushrooms are used as an
auxiliary material. The quantities of important minerals generally found in wild growing fungi;
Sodium 100-400, Potassium 20,000-40,000, Calcium 100-500, Chlorine 1000-6000, Magnesium 800-
T

1800, Phosphorus 5000-10000 and Sulfur 1000-3000 mg/kg of arsenic are present. It also contains
iron 30-150 and selenium 0.5-20 mg/kg. Mushrooms contain different secondary metabolites such as
O

phenolic compounds, terpenes and steroids. Fungal phenolics are both an excellent antioxidant and a
non-mutagenic active chemical component and it has been determined that there is a correlation
between the antioxidant activities of some edible fungi and the total amount of phenolic compounds.
N

Fungi are widely found in fat, carbohydrate, fiber, protein, essential amino acids and other amino
acids, some minerals important for nutrition (P, K, Na, Ca, Mg, Fe, Mn, Zn, Cu). It contains
substances such as thiamin, riboflavin, niacin, tocopherol, vitamin D, flavor and taste compounds,
antioxidants as well as organic compounds (lectin, adustine, carboxymethyl) and toxic elements (As,
Hg, Cd, Pb) and ribonuclease and nicotine. It is possible to find fungi containing toxic minerals in
large quantities depending on the growing and sometimes growing medium. Minerals in fungi
compositions are not available in fungal compositions unless they have toxic-acting minerals in their
compost compositions prepared from the environment in which they are grown. In the compositions of

Corresponding Author: HSEYN GENCELEP, genccelep@hotmail.com

1
ONDOKUZ MAYIS NVERSTES

1124
the fungi, globulins, glutens, prolamins and other protein variants were also identified. The total free
amino acid amounts of fungi in the dry matter content were determined to be between 1.5 and 72 g/kg.
Glutamic acid was found in high amounts at 37.6 g/kg in dry matter, although methionine was
determined to a very limited extent. Carbohydrates constitute half of the content of fungal dry matter.
Carbohydrates are composed of various compounds: sugars (monosaccharides, derivatives and
oligosaccharides) contain both reserve and building polysaccharides (glycans). As a result of studies
on compositions of browned wild mushrooms, it has been determined that the compositions of the
fungi vary widely depending on the medium they are grown, and that they contain very different
substances in their composition. It has been shown that it is possible to produce foodstuffs with
functional properties by joining alone or in combination with various foods, as it is possible to have a
long shelf life with the operations to be carried out by drying.

KEYWORDS
Edible wild mushroom, functional food, mushroom composition

1125
 Poster Session 8
Submission ID: 1057

NATURAL MEDICINE FOR THE TREATMENT OF OBESITY:

SAFFRON (CROCUS SATIVUS L.)

1 1 1
JIYAN ASLAN CEYLAN , BRA BAPINAR , AYE ZFER ZELK

ABSTRACT
Plants have been used to treat many diseases throughout the history of humanity. The World
Health Organization (WHO) reports that they have been using herbal drugs in the first place to
eliminate the health problems of nearly 4 billion people (80% of the world population) in the world.
Grown in very narrow areas in Karabk (Safranbolu) and anlurfa (Harran plain) in Turkey, saffron
(Crocus sativus L.) is a cormous plant with a high economic value among medicinal and aromatic
plants in world markets. Saffron has three main metabolites: piccrocines, responsible for the bitter
taste of saffron; safranal, a volatile oil responsible for aroma; and crocins, from carotene family that
responsible color of saffron. Saffron, especially its crosin and safranal components, has important
pharmacological characteristics such as antioxidant, anti-tumor, anti-diabetic, anti-inflammatory, and
anti-atherosclerotic. Despite the large number of studies investigating various medical characteristics
of saffron and its components, its potential for anti-obesity has not been adequately studied. It is
thought that saffron and crocin might have significant clinical effects in terms of treatment and
prevention of obesity since they help lose body weight due to the anorectic effect they have. This
study aims to evaluate the potential role of saffron and its components on the pathophysiology of
obesity.

KEYWORDS
Saffron, Crocin, Obesity, Medicinal plants

Corresponding Author: JYAN ASLAN CEYLAN, jynaslan@gmail.com

1
ANKARA NVERSTES SALIK BLMLER FAKLTES

1126
 Poster Session 8
Submission ID: 1058

ETHNOPHARMACOLOGICAL FEATURES OF ROSA CANINA L.

(ROSACEAE)

1 2
NAGEHAN SALTAN , ZGR DEVRM CAN

ABSTRACT
ETHNOPHARMACOLOGICAL FEATURES OF ROSA CANINA L. (ROSACEAE)
Nagehan SALTAN1, zgr Devrim CAN2 1Anadolu University, Faculty of Pharmacy, Department of
Pharmaceutical Botany, 26470, Eskiehir, Turkey 2Anadolu University, Faculty of Pharmacy,
Department of Pharmacology, Eskiehir, Turkey (ndagdeviren@anadolu.edu.tr) Rosaceae is a family
of flowering plants whose members are of great economic value and are traditionally used for the
treatment of several diseases in Turkey. Rosa canina L. (brier, dog rose, hip tree) is a plant of Rosa L.
genus belonging to this family. This plant is widely distributed in particular regions of Europe, Asia,
the Middle East, and South and North America [1]. In these countries, Rose hip is used as a nutritional
supplement especially due to its immune system strengthening effect [2]. In Turkey, R. canina is
known as kuburnu, yabani gl, gl burnu, gl elmas, it burnu, it gl and used for the treatment of
digestive, urinary, circulatory, respiratory system disorders, of inflammatory diseases [3]. In this
study, we reviewed the previous literature reporting the pharmacological effects of R. canina with the
aim of evaluating its therapeutic potentials on various disorders. For this purpose, we searched the
electronic databases to collect the data and reviewed more than 95 papers published between 1997 and
2017. We focused on in vitro studies, case reports, pre-clinical and clinical papers related to the
pharmacological effects of R. canina. Results of our detailed search indicated that one of the well-
known pharmacological effect of R. canina is its anti-inflammatory activity [4,5]. In addition,
protective and therapeutic effects of this plant on diabetes and diabetes induced complications are also
described, previously [6,7]. Furthermore, this plant has also been reported for its antioxidant [8],
antiproliferative [8], anticancer [9], anti-obesity [10], anti-ulcerogenic [11], hepatoprotective [12],
actinociceptive [13], and immunomodulatory [14] activities. In conclusion, results of the scientific
reports seem to confirm that R. canina has a notable therapeutic potential on various diseases.
However, further detailed clinical studies focusing on efficacy and toxicity of this plant, are strongly
needed. Key words: Ethnopharmacology. Rosa canina, Rosaceae References [1] Nilsson O., Rosa. In
P.H. Davis (Ed.), Edinburgh: Edinburgh University Press, 1997, (pp. 106128). [2] Eteghad, SS.,
Nasrabadi, HT., Aghdam, Z., Zarredar, H., Shanehbandi, D., Khayyat, L., Piran, SHS., BioImpacts
,2011, 1(4),219-224. [3] Hayta, ., Polat, R., Selvi, S., J, Ethnopharmacol., 154 , 2014, 613623. [4]
Cheng BC, Fu XQ, Guo H, Li T, Wu ZZ, Chan K, Yu ZL. Pharmacol. Res. 2016; 114: 219-34. [5]
Lattanzio F, Greco E, Carretta D, Cervellati R, Govoni P, Speroni E. J. Ethnopharmacol. 2011; 137:
880-5. [6] Farajpour R, Sadigh-Eteghad S, Ahmadian N, Farzipour M, Mahmoudi J, Majdi A. Med.
Princ. Pract. 2017, in press. [7] Taghizadeh M., Rashidi A., Taherian A., Vakili Z., Sajadian MS.,
Ghardashi M. Journal of Evidence-Based Complementary & Alternative Medicine 2016; 21: NP25-30.
[8] Jimnez S, Gascn S, Luquin A, Laguna M, Ancin-Azpilicueta C, Rodrguez-Yoldi MJ. PLoS

Corresponding Author: NAGEHAN SALTAN, ndagdeviren@anadolu.edu.tr

1
ANADOLU NVERSTES ECZACILIK FAKLTES FARMASTK BOTANK ABD
2
ANADOLU NVERSTES ECZACILIK FAKLTES FARMAKOLOJ ABD

1127
One. 2016; 11: e0159136. [9] Tumbas VT, Canadanovi-Brunet JM, Cetojevi-Simin DD, Cetkovi
GS, Ethilas SM, Gille L. J Sci Food Agric. 2012; 92: 1273-81. [10] Nagatomo A, Nishida N, Fukuhara
I, Noro A, Kozai Y, Sato H, Matsuura Y. Diabetes Metab Syndr Obes. 2015; 8: 147-56. [11] Grbz I,
Ustn O, Yesilada E, Sezik E, Kutsal O. J, Ethnopharmacol. 2003; 88: 93-7. [12] Sadeghi H,
Hosseinzadeh S, Akbartabar Touri M, Ghavamzadeh M, Jafari Barmak M, Sayahi M, Sadeghi H.
Avicenna J. Phytomed. 2016; 6: 181-8. [13] Deliorman Orhan D, Harteviolu A, Kpeli E, Yesilada
E. J. Ethnopharmacol. 2007; 11: 394-400. [14] Sadigh-Eteghad S, Tayefi-Nasrabadi H, Aghdam Z,
Zarredar H, Shanehbandi D, Khayyat L, Seyyed-Piran SH. Bioimpacts 2011; 1: 219-24.

KEYWORDS
Ethnopharmacology. Rosa canina, Rosaceae

1128
 Poster Session 8
Submission ID: 1059

ANTIMICROBIAL ACTIVITY OF ESSENTIAL OILS EXTRACTED

FROM THYME, ROSEMARY AND LAUREL AGAINST FISH
PATHOGENIC MICROORGANISMS

1 1 1 2
SERPIL TURAL , YKSEL DURMAZ , EDA URAR , SADETTIN TURHAN

ABSTRACT
In this study, the antimicrobial activity of thyme (Thymus vulgaris L.), rosemary (Rosmarinus
officinalis L.) and laurel (Lauris nobilis L.) essential oils against Yersinia ruckeri, Lactococcus
garvieae, Pseudomonas fluorescens and Aeromonas sobria were investigated. The essential oils were
extracted by hydro-distillation using a Clevenger apparatus, and their antimicrobial activities were
measured by paper disc diffusion method. All essential oils used in this study showed antimicrobial
activity against test microorganisms. The highest antimicrobial activity against Y. ruckeri, L. garvieae,
P. fluorescens and A. sobria was determined in thyme essential oil with zone diameters of 31.50,
29.50, 26.50 and 31.50 mm, respectively. The antimicrobial activity of rosemary and laurel essential
oils against all test microorganisms (except L. garvieae) was similar. In general, P. fluorescens was
less sensitive to the inhibitory activity of thyme, rosemary and laurel essential oils than other test
microorganisms. These results suggested that thyme, rosemary and laurel essential oils can be used as
antimicrobial agents against fish pathogenic bacteria.

KEYWORDS
Antimicrobial activity, thyme essential oil, rosemary essential oil, laurel essential oil, fish
pathogenic bacteria

Corresponding Author: SADETTN TURHAN, sturhan@omu.edu.tr

1
GIDA TARIM VE HAYVANCILIK BAKANLII, VETERNER KONTROL ENSTTS, SAMSUN
2
ONDOKUZ MAYIS NVERSTES

1129
 Poster Session 8
Submission ID: 1062

PREPURIFICATION OF LIPASE ENZYME FROM CORIANDER SEED

(CORIANDRUM SATIVUM)

1 1
DUYGU MERCAN , MGE GIDI

ABSTRACT
Coriander (Coriandrum sativum) is an aromatic plant, and due to its therapeutic properties, it
is widely used in many fields such as food, medicine, perfumery and cosmetics. Coriander is a
medicinal and aromatic plant that is cultured in our country. It has been used for a long time due to
appetising, , carminative and digestive properties in folk medicine. In recent years, studies on the
pharmacological effects of coriander have gained intensity. Starch, tannins, sugars, fixed and essential
oils are found in the seeds of coriander. in its fruit bearing seed. There is high incidence of coriandrol
and low incidence of geraniol, borneol, pinene, phelladron and acetic acid in the essential oil of
coriander. Lipases can be produced by animals, microorganisms and plants, and are enzymes that
hydrolyze mono-, di- and triglycerides in the oil-water phase. Lipases have an important place in
industry and medicine as they show activity in both aqueous and anhydrous solvent systems. Herbal
lipases are preferred in industry and medicine because their accessibility and low cost. Lipases are
pancreas enzyme that can digest lipids mechanically. Coriander seeds are used because of digestive
properties colloquially. In this regard, whether coriander seed helps to digest lipids will be determined
by the lipase activity. In this study, purification of lipase from coriander seed was done to add new
literature of coriander. The plant was first degreased with acetone. After drying process, the phosphate
buffer was applied in the refrigerator for 12 hours. The clear part of the centrifuged extract was
saturated with ammonium sulphate up to 80%. Olive oil was used as substrate for enzyme activity
measurements. The maximum activity in enzyme activity measurements was at the addition of 0.8 ml
of base at 70% and 80% saturation.Enzyme activity results were found to be lower than the other
medicinal aromatic plant seeds lipase purification results.

KEYWORDS
Coriander seed, Lipase, Purification, Medicinal Plant

Corresponding Author: MGE GD, mugegidis@gmail.com

1
DUMLUPINAR NVERSTES

1130
 Poster Session 8
Submission ID: 1064

DETERMINATION OF PHENOLIC COMPOUNDS IN FRAXINUS

EXCELSIOR AND FRAXINUS AMERICANA LEAVES BY LIQUID
CHROMATOGRAPHY-ELECTROSPRAY TANDEM MASS
SPECTROMETRY

1 1 1
BUSE AYDOAN , MUSTAFA CITTAN , ALI ELIK

ABSTRACT
Fraxinus (family Oleaceae) is a genus of approximately 50 species of hardwood trees and
shrubs found in Europe, N. Africa, W. Asia and N. America. Important North American species
include Fraxinus americana (white ash) [1]. Fraxinus excelsior, also known as the common ash, is a
species that grows in our country. The leaves of the both species, like all other oleaceae family,
contain significant amounts of phenolic compounds. Phenolic compounds are widespread secondary
plant metabolites. The most important classifications of phenolic compounds in oleaceae family are
secoiridoids, phenyl ethyl alcohols, flavonoids and phenolic acids. There is currently much interest in
these phytochemicals as bioactive components of foodstuffs [2]. Many of these phytochemicals
possess significant antioxidant capacities that are associated with lower occurrence rates of several
human diseases [3]. The purpose of this study was to evaluate the phenolic contents of leaves extracts
of Fraxinus excelsior and Fraxinus americana obtained with infusion (IE) and ultrasound-assisted
extraction (UAE) techniques. IE and UAE were carried out with ultrapure water and methanol/water
solution (70/30, v/v), respectively. 34 phenolic compounds were scanned by liquid chromatography-
electrospray tandem mass spectrometry (LC-ESI-MS/MS) and 22 of them were quantitatively
determined in the leaves extracts. Contents of phenolic compounds in the extracts varied from 0.59 g
g-1 to 55.2 mg g-1. Oleuropein, a secoiridoid, was the dominant compound in both extracts of two
species. Other predominant compounds were identified as verbascoside and hesperidin. Oleuropein is
a very powerful antioxidant and its amount in the leaves of Fraxinus species that reaches
approximately 5.5% by weight is quite remarkable. Consequently, the high amounts of oleuropein,
verbascoside and hesperidin extracted from ash leaves make this raw material a key source of these
phenolic compounds. References [1] N. Hammatt, Fraxinus excelsior L. (Common Ash), T. Widholm,
Jack M., Kumlehn, Jochen, Nagata, Ed. Berlin: Springer, 1996, pp. 172193. [2] D. trbov, D.
Matj ek, J. Vlek, and V. Kub, Anal. Chim. Acta, 513(2), 435444, 2004. [3] K. J. Anderson, S.
S. Teuber, A. Gobeille, P. Cremin, A. L. Waterhouse, and F. M. Steinberg, J. Nutr., 131(11), 2837
2842, 2001.

KEYWORDS
Fraxinus excelsior, Fraxinus americana, Oleaceae, phenolic compounds, LC-ESI-MS/MS

Corresponding Author: MUSTAFA CTTAN, mustafa.cittan@cbu.edu.tr

1
MANSA CELAL BAYAR NVERSTES

1131
 Poster Session 8
Submission ID: 1065

INVESTIGATION OF PHENOLIC COMPOUNDS AND TOTAL

PHENOLIC CONTENT OF ISABELLA GRAPE (VITIS LABRUSCA L.)
BERRIES IN BARTIN REGION

1 1 1 2
BRAHIM TMEN , DILEK NE , HASAN KESKN , MEHMET KURTA

ABSTRACT
Vitis labrusca L. which is known as Aromatic grape, aromatic black grape, strawberry grape,
isabella, American grape, black grape grows in in Black Sea Region coastline. It has a wrapping
body, a berry with special aroma, thick skin and seed. It grows very fast and it can grow up to 15
meters. It had been determined that. V. Labrusca berry and leaves has phenolic compounds, organic
acids, vitamins and minerals, enzymes, monosaccharides, nitrogenous compounds, terpenes and lipids
in phytochemical researches upon this plant. According to research upon V. labrusca, this plant has
healing effects on respiratory tract infection, heart attack, cholesterol, digestive system disorders, some
cancer species and Alzheimer. This also shows that V. labrusca is very important plant medically. In
this study, berries of isabella grape (V.labrusca L.) which was collected from Bartin city, Serdarli
Village, were used. Three different samples were prepared as fresh, dried in outdoor, dried in drying
oven. Phenolic compounds of Isabella grape (V.labrusca L.) were identified by HPLC. Total phenolic
content was determined by Folin-Ciocalteu reagent (FCR). According to results of phenolic
compounds analysis, in all of the fresh sample, sample dried in outdoor and sample dried in drying
oven, the highest amount was determined as vitamin C (respectively; 11.205 mg/L, 15.191 mg/L,
15.160 mg/L). When compared these three samples, the highest total phenolic contents were
calculated in sample dried in drying oven.

KEYWORDS
Phenolic compounds, isabella grape, HPLC

Corresponding Author: BRAHM TMEN, tumen@bartin.edu.tr

1
BARTIN NVERSTES ORMAN FAKLTES
2
BARTIN NVERSTES SALIK HZMETLER MESLEK YKSEKOKULU

1132
 Poster Session 8
Submission ID: 1067

PLACE AND IMPORTANCE OF MEDICINAL AND AROMATIC

PLANTS IN THE WORLD AND TURKEY

1 2
VEYSI ACIBUCA , DILEK BOSTAN BUDAK

ABSTRACT
In agricultural activities, as well as the production of the necessary products to meet the needs
such as nutrition, dressing, and sheltering which are vital for mankind; there also produced products
that are useful for human health. It is known that plants with therapeutic properties have been used in
diseases since the beginning of human history. In the early days, only some of these plants, which
were collected from nature for nutrition and treatment purposes, were cultured and produced for
economic purposes. In addition to therapeutic uses of medicinal and aromatic plants; they are used in
many areas such as food, cosmetics and aroma. As a result of agricultural products trades gaining of
international qualification, and the increase in demand for these plants; a rise in the production and
collection of medicinal and aromatic plants has happened. According to the World Health
Organization data, while 80% of the population uses traditional medicines for treatment in
underdeveloped countries; this proportion is around 40% in developed countries. And it is expected
that the rate of utilization of medical plants in the future will increase all over the world. Due to its
location and climate conditions, our country has a wide variety of plant species and is one of the
leading producer countries of many medical and aromatic plants. For this reason, medical and
aromatic plants have an important economic potential for our country. In this study, production and
trade of medical and aromatic plants in the world and in Turkey have been examined in line with the
data obtained from national and international institutions and previous studies related to the subject. In
addition, the support given directly and indirectly to the medical and aromatic plants in Turkey has
been examined and suggestions have been presented to increase the share of our country in the world
trade.

KEYWORDS
World, Turkey, Medicinal Plants, Aromatic Plants

Corresponding Author: VEYS ACIBUCA, veysacibuca@gmail.com

1
MARDN ARTUKLU NVERSTES
2
UKUROVA NVERSTES

1133
 Poster Session 8
Submission ID: 1068

EVALUATION OF AVACADO (PERSEA AMERICANA MILL.)

LEAVES IN TERMS OF PUBLIC HEALTH

1 1
GLSEN KENDIR , AYEGL KROLU

ABSTRACT
Persea americana Mill. (Lauraceae) originated in central and southern of South America, is a
evergreen tree. The tree commonly known as avocado. Avacado is cultivated in all tropical and
subtropical regions in the world. This tree has been usually grown in southern coastal region of Turkey
due to its commercial importance. Its fruit is a drupe which consumed as food. Except this, the leaves
are widely used for pass kidney stone and against the urinary tract infections as therapeutic among the
people in Turkey and Cyprus. This study was made to determine if they have scientific drug
characteristics which used for its therapeutic feature in folk and supplied from the market of avacado
leaves. For this purpose, 13 different samples from 5 diverse cities (Ankara, Hatay, stanbul, Kayseri
and Aydn) were purchased. The sample to be used as standard was obtained from the culture form.
The morphological properties of leaves, which are part of the plant used as drug, were determined both
in the standard sample and in the purchased samples. Anatomical features of transverse and surface
sections from standard avacado leaves with transverse sections from the petiole of natural avacado
leaves were investigated and determined. Besides, the distinctive anatomical structures of the
powdered samples (standard sample and samples purchased from the market) were demonstrated. The
avacado leaf is bifacial. The hairs are only located lower surface of the leaf and mostly unicellular.
Secretory cells and crytals were observed in the avacado leaf and petiole. Crystals are simple in
various sizes, and small raphids. The stomata confined to the lower surface and anomocytic type (3-6
subsidiary cells). In tranverse section taken from the petiole, the hairs were observed to be gathered on
the upper surface of petiole. References 1) Yasir, M., Das, S., Kharya, M. D. (2010). The
phytochemical and pharmacological profile of Persea americana Mill. Pharmacognosy Reviews, 4(7):
77-84. 2) Gruenwald, J., Brendler, T., Jaenicke, C. (Scientific Editors) (2004). Physicians desk
reference (PDR) for herbal medicines. Thirth Edition. Thomson/Medical Economics Company,
Montvale, New Jersey. 3) Sargin, S. A. (2015). Ethnobotanical survey of medicinal plants in Bozyazi
district of Mersin, Turkey. Journal of Ethnopharmacology, 173: 105-126. 4) Demirkol, A. (1995).
Avocado growing in Turkey. Proceedings of the World Avocado Congress III, p. 451-456.

KEYWORDS
Persea americana, Lauraceae, Morphology, Anatomy, Powder drug

Corresponding Author: GLSEN KENDR, kendir80@hotmail.com

1
ANKARA NVERSTES

1134
 Poster Session 8
Submission ID: 1069

COMPOSITION OF THE ESSENTIAL OILS OF TWO INULA SPECIES

(I. SALICIANA L. AND I. DISCOIDEA BOISS.) GROWING WILD IN
TURKEY

1 2 3
M. YAVUZ PAKSOY , EYP BACI , FETHI AHMET ZDEMIR

ABSTRACT
The Inula genus members are mostly herbs or subshrubs, but sometimes annual or biennial
herbs and belongs to the Asteraceae family. Several species of Inula are used in traditional medicine as
an extensively used primarily for treatment of abdominal pain, emesis, diarrhea, and threatened
abortion. In addition, the roots are effective in the treatment of diuretic, diaphoretic, expectorant, and
anthelmintic remedy. In this study, the essential oil composition of Inula saliciana L. and Inula
discoidea Boiss. collected from Turkey were analyzed. The oils obtained by hydrodistillation using
Clevenger aparatus from two Inula species and chemical composition were determined by GC and
GC-MS system. The oil yield were determined as 0.3% and 0.4% (v/w) in the both essential oils
respectively. Sixteen constituents were comprised the 94.5% of the total essential oil extracted from
the Inula saliciana. The predominant compounds of the oil were determined as 2-pentadecanone
(30.5%), tetradecanal (25.5%) and [+]-Epi-bicyclosesquiphellandrene (7.0%). On the other hand,
tetradecanal (38.0%) and palmitic acid (19.0%) were found as the main compounds identified in the
oil of Inula discoides.

KEYWORDS
Inula, GC-MS, Essential oil, Turkey.

Corresponding Author: M. YAVUZ PAKSOY, mypaksoy@gmail.com

1
MUNZUR NVERSTES
2
FIRAT NVERSTES
3
BNGL NVERSRES FEN-EDEBYAT FAK./ MOLEKLER BYOLOJ VE GENETK BLM

1135
 Poster Session 8
Submission ID: 1070

A TRADITIONAL TURKISH DESSERT: PEKMEZ

1 2
EMS GL YILMAZ , ASLI UAR

ABSTRACT
In Turkey, pekmez (liquid pekmez, LP) is one of the traditional food products and it is
commonly produced from grape and can also be produced from fruits containing high amounts of
sugar like apple, carob, plum, watermelon, apricot, sugar beet, mulberry and fig. For this reason in
Turkey when the pekmez is called the first come to mind is grape pekmez. Pekmez is generally
defined as boiling concentrated or shelf-life-preserved mulberry or grape juice without adding sugar or
other food additives. Grape is the most commonly used fruit in pekmez production. Grape (Vitis
vinifera L.) is one of the worlds largest fruit crops, with an approximate annual production of 58
million metric tons. About 37% of harvested grapes are used in pekmez production in Turkey. Also,
about 657,000 tons of grapes are processed into pekmez annually in Turkey. Grape pekmez is
produced from grapes or raisins in an open kettle or vacuumed tank. Pekmez is traditionally made in
rural areas. However, commercial pekmez production in small to mid-sized plants has been on the rise
in recent years. Grape pekmez is a thick, concentrated product and is produced from grape or raisin
paste by decreasing its acidity. Vacuum application may be applied to concentrate the product. The
purpose of concentration is to extend the shelf-life by reducing the water content and to pasteurize the
grape juice. Local pekmez generally takes its name from the place it is made such as Zile pekmez, the
name of a Turkish town. Pekmez has been produced in Turkey for a long time in appreciable amounts
and its production technology has changed very little since it was first produced. Pekmez made in
Turkey is either in the liquid or in the solid form. Turkish standards classify pekmez as sweet (pH
between 5 and 6) and sour (pH between 3.5 and 5.0). In the traditional production of sweet pekmez,
grapes are washed and pressed to obtain grape must. The resultant must is cloudy and acidic. Grape
must mainly contains tartaric, malic and citric acid, with tartaric acid being predominant. To neutralise
acidity of grape must, pekmez earth can be used in percentages ranging from 0.1% to 1%. It is
generally known as pekmez, and it is a healthy and natural product. Pekmez is consumed mainly for
breakfast instead of jam or marmalade Pekmez contains high amounts of sugar,mineral and organic
acid, so, it is a very important food product in human nutrition especially for babies, children, and
sportsmen and in situations demanding urgent energy. Pekmez has an important function in the
working of the brain in which glucose is an energy source. Pekmez easily passes into the blood
without digestion because most of its carbohydrate is in the form of monosaccharide like glucose and
fructose.

KEYWORDS
Nutrition, Traditional Food, Pekmez, Production, Grape

Corresponding Author: EMS GL YILMAZ, sgyilmaz@ankara.edu.tr

1
ANKARA NVERSTES

1136
 Poster Session 8
Submission ID: 1072

A REVIEW OF NURSING THESES ON AROMATHERAPY IN

TURKEY

1 1
LKNUR MNEVVER GNEN , NAZAN AKIRER ALBAYRAM

ABSTRACT
Purpose: This study aims to review the nursing theses carried out on aromatherapy in Turkey.
Method: To collect the research data, first a literature review was conducted, and then a key word
search was carried out with keywords such as aromatherapy, aromatherapic, and of aromatherapy
in the database of the National Dissertation Center of the Higher Education Council. As a result of the
search, 23 theses from Health Sciences Institute, Nursing Department related to the subject in question
were identified, and the citation details of these theses were obtained. Findings: Of the theses
conducted on aromatherapy in the field of nursing, 5 were masters theses, and 18 were doctoral
dissertations. 10 of these theses were carried out in Internal Medicine Nursing Department, 3 in
Surgical Diseases Nursing Department, 4 in Fundamentals of Nursing Department, 2 in the
Department of Child Health and Diseases Nursing, 2 in Women's Health and Obstetric Nursing
Department, and 2 in Public Health Nursing Department. The research data in 10 of the studies were
conducted as randomized controlled study. In 11 of the theses, aromatherapy was administered
cutaneously, by inhalation in 9, and both cutaneously and through inhalation in 3. Lavender was the
most commonly used aromatherapy oil in these studies. The effect of aromatherapy on more than one
factor was studied in these studies. The first five items in terms of aromatherapy use were found to be
coping with pain, coping with anxiety, improving sleep quality, reducing fatigue, and boosting life
quality respectively. All of the theses found that aromatherapy agents were effective. Conclusion: It is
noteworthy that aromatherapy has been used in many areas of nursing, including mainly in internal
medicine nursing. This indicates that aromatherapy is one of the major areas of interest in nursing
research. It also reflects the fact that it has the capacity to be integrated into nursing care in many areas
of nursing. That nearly half of the studies were conducted as randomized controlled study increases
the reliability of the research findings. It is important to recognize aromatherapy in nursing practices
and authorize nurses with this regard. It is predicted that research into this area might grow if the
nurses are authorized. It is believed that the outcomes of this study will guide nurses and other health
professionals who are interested in studying on aromatherapy.

KEYWORDS
Aromatherapy, nursing, care

Corresponding Author: LKNUR MNEVVER GNEN, imgonenc@gmail.com

1
ANKARA NVERSTES SALIK BLMLER FAKLTES

1137
 Poster Session 8
Submission ID: 1073

DETERMINATION OF ZN(II) AND CD(II) CONCENTRATION IN

WHEAT THAT GROWN AT KARAMAN WITH VOLTAMMETRIC
METHODS

1 1
AYSEL MEN , ALI BLG

ABSTRACT
Toxic and persistence substances in the environment continuously accumulate and increase
owing to the anthropic activities. With reference to this, one of the major problems in ecology is
related to the path of toxic metals contained in all matrices involved in foods and food chain, because
of their irreversible deleterious effects on man [1]. The removal of these toxic materials is crucial for
the living and the people. For this reason, heavy metals were determinated using voltammetric
methods on the wheat grown in Karaman. Three group of crop wheat were gathered from Karaman-
Konya, Karaman-Organized Industrial zone motorway and some villages of Karaman, respectively.
These samples were crushed at laboratory mill to obtain whole wheat flour and then sieved with 250
micron sieve. So, some whole wheat flour sample was converted into wheat flour for analyses. All
samples were prepared after melting with mixture of 10 mL 98% H2SO4 + 10 mL 35% HNO3 + 10
mL 30% H2O2 at microwave oven. Optimized conditions were determined for Anodic Stripping
Voltammetry method. Calibration curves were plotted for analyses of Zn(II), Pb(II). After that,
standard metal mixtures which were prepared with standard addition method were added in to both of
whole wheat flour and sieved wheat flour. So those samples were analyzed. According to these results;
those samples do not include Cd(II). Also determined amount of Zn(II) below to the limit of danger
for human health.

KEYWORDS
Anodic stripping voltammetry, Polarography, Whole wheat flour, Heavy metal.

Corresponding Author: AL BLG, alibilgic100@hotmail.com

1
KARAMANOLU MEHMETBEY NVERSTES

1138
 Poster Session 8
Submission ID: 1074

DETERMINATION OF PB(II) AND CU(II) CONCENTRATION IN

WHEAT THAT GROWN AT KARAMAN

1 1
AYSEL MEN , ALI BLG

ABSTRACT
Plants pick up heavy metals from the ground with their roots and leaves from the air. Plants
planted with industrial wastewater contain more heavy metals. Toxic effect occurs when the human
body is exposed to heavy metals over certain quantities [1]. For this reason, heavy metals were
determinated using voltammetric methods on the wheat grown in Karaman. Three group of crop wheat
were gathered from Karaman-Konya, Karaman-Organized Industrial zone motorway and some
villages of Karaman, respectively. These samples were crushed at laboratory mill to obtain whole
wheat flour and then sieved with 250 micron sieve. So, some whole wheat flour sample was converted
into wheat flour for analyses. All samples were prepared after melting with mixture of 10 mL 98%
H2SO4 + 10 mL 35% HNO3 + 10 mL 30% H2O2 at microwave oven. Optimized conditions were
determined for Anodic Stripping Voltammetry method. Calibration curves were plotted for analyses of
Cu(II), Pb(II). After that, standard metal mixtures which were prepared with standard addition method
were added in to both of whole wheat flour and sieved wheat flour. So those samples were analyzed.
According to these results; those samples do not include Pb(II). Also determined amount of Cu(II)
below to the limit of danger for human health.

KEYWORDS
Anodic stripping voltammetry, Whole wheat flour, Heavy metal.

Corresponding Author: AL BLG, alibilgic100@hotmail.com

1
KARAMANOLU MEHMETBEY NVERSTES

1139
 Poster Session 8
Submission ID: 1075

EFFECTS OF GOJI BERRY (LYCIUM BARBARUM) EXTRACT ON

EXPERIMENTAL TOXOPLASMOSIS

1 1 1 1 1
EROL AYAZ , FATMA PEHLIVAN KARAKA , KEREM YAMAN , AYHAN ETINKAYA , HAYRIYE ORALLAR , ENES
1
EILMEZ

ABSTRACT
Effects of Goji berry (Lycium barbarum) Extract on Experimental Toxoplasmosis Erol
AYAZ1, Fatma PEHLVAN KARAKA2, Kerem YAMAN1, Ayhan ETNKAYA3, Hayriye
ORALLAR4, Enes ELMEZ5 1 AB Medical Faculty, Department of Parasitology, BOLU 2
AB Agriculture and Natural Sciences Faculty, Division of Medical and Aromatic Plants, BOLU 3
AB Medical Faculty, Department of Physiology, BOLU 4 AB Agriculture and Natural Sciences
Faculty, Division of Poultry, BOLU 5 AB Lab Animals Application and Research Center, BOLU
Aim of our study is to examine the anti-parasitic effects of Lycium barbarum extract on the
experimental model of toxoplasmosis. Lycium barbarum is known as goji berry or wolf berry and used
in traditional Chinese medicine as an anti-oxidant, immunostimulator, relaxing and possible an
additive agent against cancer. The polisaccharides of L.barbarum fruit is mostly responsible for these
effects. Toxoplasma gondii is an intracellular Apicomplexan parasite which has got a worldwide
spread. Toxoplasmosis isnt a symptomatic infection but it may threat life and cause severe conditions
at immunocomprimised individiuals, fetuses and newborns. Separate from the known effects of
L.barbarum , no study is accomplished as an anti-parasitic agent for this plant. Method: We used
methanol evaporated and lyophilised essence of L.barbarum fruits. We applicate this extract to 40
mice aged 2-4 months old in this study. Mice were divided into five groups according to their situation
of infection and intragastric application of extract. All mice in chosen groups were infected with
1X105 tachyzoites of T.gondii RH strain. As an antiparasitic agent, 24 mg/ml dosage of co-
trimoxsazole in saline, is chosen and dispensed 0.1 ml by oral gavage . Dosage of L.barbarum extract
was adjusted 100 mg/kg in saline and dispensed 0.1 ml by oral gavage too. Group I was the healthy
group without any intervention. Group II was infected with T. gondii tachyzoites. Group III was
infected and treated with co-trimoxsazole. Group IV was infected and treated with L.barbarum extract.
Last group was the group, we both appllied the plant extract and co-trimoxsazole. After, chosen
groups were infected, we observed the groups and examined the parasite load by counting tachyzoites
in the intraperitoneal fluid of mice at Thoma chamber. Results: Significant difference is found in study
groups compared with control groups. Number of parasites found close in study groups, thus resulting
no significant difference between them. As a result of our study, we decided the extract of L.barbarum
may be helpful for treatment of toxoplasmosis. As an advise, more elaborate research may progress
through using different species of parasites.

KEYWORDS

Corresponding Author: KEREM YAMAN, keremyamantbb@yahoo.com.tr

1
ABANT ZZET BAYSAL NVERSTES

1140
Toxoplasma gondii, Lycium barbarum, Mice, Anti-parasitic

1141
 Poster Session 8
Submission ID: 1076

CYTOTOXIC, MORPHOLOGIC AND APOPTOTIC EFFECTS OF

PROTOCATECHUIC ACID ON DU145 CELL LINE

1 1
PINAR ZTOPCU-VATAN , EMINE NAN

ABSTRACT
Protocatechuic acid (PCA) is a phenolic acid and widely found spread throughout in many
plants as an aromatic secondary metabolites. Previous studies, have shown different biological and
pharmacological activities PCA, as well as, suppression of proliferation of cancer cells. Prostate
cancer is one of the most common cancer types in men and the treatment are very limited. In this
study, we determinate the cytotoxic and apoptotic effects of PCA on human prostate cancer (DU145)
line. The cytotoxic effects of PCA (0.5 to 3.5 mM) was examined in cells for 24 and 48 h by MTT and
Neutral Red (NR) assay. All statistical analyses were performed using one-way analysis of variance
(ANOVA) and followed up by Tukeys multiple comparison tests. Morphological changes in cells
were evaluated by inverted microscope. Apoptotic cell death was assessed in cells treated with 1 and
1.5 mM PCA by DAPI staining. The cell viability started to decrease at 1 mM (p<0.001) for 24 h, 0.75
mM (p<0.001) for 48 h. The IC50 values at 24 and 48 h were estimated as 1.29 and 0.90 mM by MTT
and 1.23 and 0.88 mM by NR assay respectively. Increased PCA doses caused circular cell
morphology, diminished the cell number as well as increased the nuclear condensation and
fragmentation on DU145 cells. We reported for the first time that PCA possess cytotoxic and apoptotic
effects on prostate cancer in a time and dose dependent manner. Further studies are needed to clarify
the mechanism of PCA induced apoptotic death. *This study was supported by Eskisehir Osmangazi
University, Scientific Research Projects Committee (Project number: 201219A104).

KEYWORDS
Protocatechuic acid, Prostate carcinoma, Cytotoxicity, DAPI, Apoptosis

Corresponding Author: PINAR ZTOPCU-VATAN, poztopcu@ogu.edu.tr

1
ESKEHR OSMANGAZ NVERSTES

1142
 Poster Session 8
Submission ID: 1078

EFFECT OF GOJI BERRY FRUIT EXTRACT ON SOME VITAMIN

LEVELS IN RATS FED HIGH FAT DIET

1 2 2 2
FATMAGL YUR , AHMET UFUK KMROLU , SINAN YILDIZ , AHMET CIHAT NER

ABSTRACT
Purpose: Goji berry has been traditionally used for centuries in Asia due to its positive effects
on health. In this study, we aimed to investigate the effect of goji berry fruit extract on Vitamin D,
Folic acid and vitamin B12 levels in rats fed high fat diets. Method: Forty Wistar-Albino healthy rats
were used as study material. The rats were divided into 4 groups, with 10 rats per group. Group 1:
control group given standard pelleted feed for 60 days, Group 2: fed with 60 days of standard pelleted
feed and 100mg / kg goji berry fruit extract, Groups 3: fed on a high fat diet for 60 days, Group 4 was
formed from rats fed a high fat diet for 60 days and 100 mg / kg goji berry fruit extract. After 60 days,
the rats were taken to the cardiac blood gel tube, and the serum was separated. Serum Folic acid, B12
and D vitamin levels were measured on an Arhcitect brand CD16000I model autoanalyzer. Result:
Serum folic acid level was significantly lower in group 3 than in the other groups. B12 vitamin and
vitamin D levels were significantly lower in Group 3 and Group 4 than Group 1 and Group 2 (p
<0.05). Conclusion: VitD deficiency and obesity are common health problems. It still has to argue
which one is the cause of the other. Vit D suppresses lipolysis by increasing the Ca ++ transition from
outside to inside the cells, it stimulates lipogenesis. The same mechanism also suppresses the
expression of uncoupling protein 2 (UCP2), which allows the use of lipids in mitochondria in the cell,
leading to lipid accumulation. In our study, we observed that there were no effects on the vitamin level
of the goji berry extract, which was reduced by Vitamin D, in the groups we fed with the oily diet.
When the folate level was lower in the group fed with the overfat diet than in the other groups, but
there was no significant difference (p), we observed that vitamin B12 levels fell in the 3rd and 4th
groups (p <0.05). Goji berry fruit extract did not affect vitamin D, B12 and folic acid levels in rats fed
high fat diets.

KEYWORDS
Goji berry, vitamins, high fat diet

Corresponding Author: FATMAGL YUR, fatmagulyur@mu.edu.tr

1
MULA SITKI KOMAN NVERSTES, FETHYE SALIK BLMLER FAKLTES, BESLENME VE
DYETETK BLM, MULA
2
YZNC YIL NVERSTES

1143
 Poster Session 8
Submission ID: 1079

STRUCTURAL INVESTIGATION OF THE GLANDULAR

TRICHOMES OF ENDEMIC MARRUBIUM CEPHALANTHUM BOISS.
& NO SUBSP. MONTANUM AKGL & KETENOLU, A NEW
SUBSPECIES FROM TURKEY

1 1 1
LKAY ZTRK ALI , ARZU CANSARAN , CENGIZ YILDIRIM

ABSTRACT
The genus Marribium L. (Lamiaceae) has more than 40 species in the world. Most of them i.e.
12 species are endemic to Turkey and more than half of them (i.e. 22 species) are found in Turkey.
Except few species, most of them are perennial herbs. There are a few species in this genus that is
known to be used for medical purposes. The genus Marrubium is characterized by erect or ascending
stems and densely hairy, usually having many lateral branches, leave with toothed margins, flowers
usually arranged densely on the stems known as verticillaster, having broad bracts and small
bracteoles, and having tubular calyx and corolla. Marrubium cephalanthum subsp. montanum is a new
endemic subspecies from Turkey. The plant specimens were collected in its flowering period from
Amasya on 20.05.2016. The plant materials were identified and deposit at Amasya University.
Structural studies of the glandular trichomes were carried out on vegetative and reproductive organs of
the plant that were fixed in 70% alcohol. Hand made superficial sections in leaves on upper and lower
surfaces and cross sections in stem, petiole, calyx, corolla were obtained to determine the glandular
trichome structures of the plant. M. cephalanthum subsp. montanum has two main types of glandular
trichomes. These are peltate and capitate glandular trichomes.

KEYWORDS
Marrubium cephalanthum subsp. montanum, Trichome, Endemic

Corresponding Author: LKAY ZTRK ALI, ilkaycali@hotmail.com

1
AMASYA NVERSTES

1144
 Poster Session 8
Submission ID: 1080

SPECIES OF GLAUCIUM FLAVUM CRANTZ (PAPAVERACEAE)

MORPHOLOGICAL CHARACTERISTICS AND SPREADING AREAS
IN TURKEY.

1 2 2
FATMA MUNGAN KILI , KEMAL YILDIZ , MURAT KILI

ABSTRACT
A bright, remarkable yellow color which belongs to the genus Glaucium Mill. it is known as
"yellow horned poppy". Species spread on the sea side, river valleys (0-50 m). The medical use;
antidiabetic, anti-cancer, antibiotic and cardioprotective effect. Field work was carried out in the
vegetation period covering the May to August months between the years of 2011 and 2015. The
species were collected from as many different locations. Records of the collected samples were kept,
photographs were taken in natural growing environments and the specimens collected were dried
according to standard procedures and transformed into herbarium specimens. Pollen and seeds of the
species were analized by scanning electron microscopy (SEM). As a result of morphological
examination, the stem is 23-42 cm lenght, hairless, branched, whitish green; the leaves are thick, very
green-matte. Flower buds are hairless or slightly acute, ovate -rectangular, acute, petals usually
yellow. As a result of the morphological investigations, parallelism was observed between the
obtained data and the Flora of Turkey. In addition, the spreading areas and description have been
improved and the obtained data has been transformed into a table. At the end of the palynological
investigation, pollen grains usually were spheroidal in shape and tricolpate aperture, ornamentation
microecinate and microperforate. Seed features; reniform, that surfaces alveolate and faveolate was
observed.

KEYWORDS
Glaucium, Morphology, Palynology, Medicinal Plant, Flora of Turkey

Corresponding Author: FATMA MUNGAN KILI, fatmamungan4747@gmail.com

1
MARDN ARTUKLU NVERSTES
2
CELAL BAYAR NVERSTES

1145
 Poster Session 8
Submission ID: 1081

GOLEVEZ (TARO, KOLOKAS)

1 1 1
AYLA NVER ALAY , EKIN DNEL , MERYEM BADAYMAN

ABSTRACT
Research into food-derived bioactive components for cancer prevention as well as cancer
therapy is growing due to the relatively low or no detectable toxicity and better bioavailability. In this
regard,it has been carried out scientific research on the plant called "golevez" in our country and "taro,
old cocoyam, eddoe or dasheen" in other countries. Golevez [Colocasia esculenta (L.) Schott] is a
tropical root vegetables from the family of the Araceae. Golevez, which is produced extensively in 43
states around the world, is grown in Alanya and Gazipaa of Antalya, Bozyaz, Anamur districts of
Mersin and is well known as "kolokas" in the Turkish Republic of Northern Cyprus and widely
consumed. The primary use of golevez is the consumption of edible leaves and root corms. The corms
can be cooked as vegetables with boiled. The leaves of golevez are eaten as vegetables by people and
are evaluated as wrapping and soup. Gollevez's corms is cooked with boiled meat, beans and chickpea.
It is used as canned food, flour, chips, noodle and frozen food in tropical and subtropical countries.
Dried fine powder of the plant's corm is used as additives for bread, pastry, food and pasta. Mucilage
of golevez participates in diet products. Oxalic acid can be present in the leaves and root corms.
Because of this, the plant must not be defeated without cooking, the oxalate ion can cause high
irritation in the digestive tract. Since the method of processing of the golevez is very unknown in our
country, it is widely consumed only in the regions where it is produced. The pharmacological effects
of the plant are also unknown in our country. However, it has been known in the world since ancient
times for its medicinal properties and is being used to help treat various diseases such as asthma,
arthritis, diarrhea, internal bleeding, neurological disorders and skin disorders. It has been found that
the extracts obtained from this plant have various pharmacological activities. Antimicrobial,
antioxidant, antiinflammatory and anticancer activity of Colocasia esculanta has been reported. It has
been reported with the scientific studies made that extracts obtained from this plant inhibit certain
tumor metastases strongly and specifically. As research on Colocasia esculanta's corm and leaves is
promising and encouraging, it is necessary to purify the active components and to carry out their
clinical studies in the future in order to increase these activities. In this review, the traditional use and
known pharmacological effects of Colocasia esculanta have been examined.

KEYWORDS
Golovez, Colocas, Colocasia esculanta, antimicrobial, anticancer

Corresponding Author: AYLA NVER ALAY, aylaalcay@aydin.edu.tr

1
STANBUL AYDIN NVERSTES

1146
 Poster Session 8
Submission ID: 1082

GRAPE SEED AS A FUNCTIONAL COMPONENT

1 1 1 1
MUTLU BUKET AKIN , ASLI ELKEL , KAMILE BURCU BYKKILI , MUSA SERDAR AKIN

ABSTRACT
Grape seed obtained after wine or juice making and contains 1319% oil, about 11% protein,
6070% of non-digestible carbohydrates,phenolic compounds, and non-phenolic antioxidants such as
tocopherols and beta carotene. The phenolic compounds in grape seeds are essentially all flavonoids,
flavan-3-ols (catechin, epicatechin and epicatechin-3-O-gallate monomers) and their polymers. Grape
seed has been used as grape seed oil or grape seed extract in food industry. Grape seed oil (also called
grapeseed oil or grape oil) is pressed from the seeds of grapes. The nutritional value of the grape seed
oil is very high because of the rich unsaturated fatty acids, mainly linoleic and oleic acids of the oil.
Grape seed oil also contains bioactive compounds including phytosterols, tocopherols, tocotrienols,
flavonoids and phenolic acids with recognized biological importance due to their antioxidant activity.
This components contribute to the beneficial effects of the grape seed oil. Particularly, grape seed oil
is a rich source of vitamin E and contains high quantities of tocopherols and tocotrienols. The grape
seed extract contains high levels of phenolic compounds including flavonoids, procyanidins and
phenolic acid. It is reported that proanthocyanidins as plant-based health-beneficial components in the
human diet have potential health beneficial effects depending on their structure and especially on their
degree of polymerization. It has been demonstrated that proanthocyanidins possess a large spectrum of
pharmacological and therapeutic benefits including antioxidant, antithrombotic, antibacterial and
antiviral, anti-inflammatory, anti-allergic and anticarcinogenic activities. Thus, due to its composition
and related properties, grape seed has emerged as a product with health-promoting effects and having
the potential for use in pharmaceutical and food applications as a functional components.

KEYWORDS
Grape seed, grape seed oil, grape seed extract, fuctional components

Corresponding Author: MUTLU BUKET AKIN, mutluakin@harran.edu.tr

1
HARRAN NVERSTES

1147
 Poster Session 8
Submission ID: 1083

ORGANIC COSMETIC PRODUCT USAGE PREVELANCE OF

WOMEN WORKING IN TRABZON

1 1 1 1
NURHAN GMRKOLU , DIDEM SARIMEHMET , SEVILAY HINTISTAN , NIHAT BURAK ZIHNI , GONCA
1
SERDAR

ABSTRACT
In this study, it was aimed to examine the prevalence of organic cosmetic product usage in
women working in Trabzon. The sample of the research made by the descriptive type is 154 women
working in different institutions in Trabzon. Data were filled with face-to-face interview technique
using the questionnaire form. When the prevalence of use of organic cosmetics in the survey is
examined; 95.5% used hand soap every day, 69.5% used shampoo once a week, 57.1% used hair dye
and hair once a month, and 3.2% used face lotion every year. Among the products that are never used;
Makeup material (3.9%), nail polish (2.6%) and shower gel (1.9%) are in the first three ranks. Brand
names (75.3%) were the most important factor in the purchase of organic cosmetics products by
working women and internet environment (51.3%) was the preferred place to purchase. As a result,
working women often use organic cosmetics.

KEYWORDS
Organic Cosmetic Product, Working Women, Internet Environment, Brand

Corresponding Author: GONCA SERDAR, goncaserdar61@gmail.com

1
KARADENZ TEKNK NVERSTES

1148
 Poster Session 8
Submission ID: 1084

SPECTACULAR FOOD-DRUG INTERACTION: ST. JOHNS WORT

(HYPERICUM PERFORATUM)

1 1
MUSTAFA FEVZI KARAGZ , MAKBULE GEZMEN-KARADA

ABSTRACT
St. Johns wort, which is distributed in hot and temperate regions all over the world, has 89
species in Turkey, the most common being Hypericum perforatum L. species. The properties of St.
Johns wort, which has antiinflamatuar, analgesic, antidepressant, antiviral, antimicrobial and
antioxidant effects, are derived from hyperforine, flavonoids, procyanidins, essential oils,
phenylpropanals, xanthones and gamma aminobutyric acid (GABA). Hipericine and quercetin have
the ability to bind to dopamine receptors, miquelianin and rutin bind to adrenergic receptors.
Hyperforin inhibits synaptosomal reuptake of serotonin, norepinephrine, GABA and dopamine.
Hence, Hypericum perforatum shows similar properties to some synthetic antidepressants (SSRI,
MAO etc.). St. Johns wort triggers the activity of cytochrome-P isozymes (CYP3A4, CYP2E1 fe
CYP2C19) and the synthesis of a carrier membrane protein, P-glycoprotein (P-gp). St. Johns wort has
been shown to increase the metabolism of many medicines, mainly by increasing the activity of
intestinal CYP3A4 enzymes. This is accomplished by activating the nuclear pregnane X receptor and
inducing CYP3A4 expression. The increase in the synthesis of P-gp leads to a decrease in drug
absorption and an increase in excretion. The hypericum extract reduces the plasma concentration of
some antineoplastic agents (irinotecan) by triggering liver metabolism of P-gp and CYP3A4. Along
with the use of St. Johns wort, the half-life of oxycodone, which is used in the treatment of patients
with chronic pain, is shortened, thereby the plasma concentration of drug is reduced. Finally,
medicines used in the treatment of diseases should be carefully considered in terms of the interaction
with the St. Johns wort and other nutrients in order not to adversely affect the treatment.

KEYWORDS
St. Johns Wort, Hypericum Perforatum, Drug Inteactons, Antidepressant

Corresponding Author: MUSTAFA FEVZ KARAGZ, mmufeka@gmail.com

1
GAZ NVERSTES SALIK BLMLER FAKLTES BESLENME VE DYETETK BLM

1149
 Poster Session 8
Submission ID: 1085

POTENTIAL HEALTH BENEFITS OF CHAMOMILE TEA

1 1 1
BRA BAPINAR , JIYAN ASLAN , AYE ZFER ZELK

ABSTRACT
Accepted as native to Europe and Western Asia, Chamomile (Chamomillarecutita) is widely
found in all regions of Turkey, and it is used as a medicinal plant in Europe. One of the most popular
herbal tea, chamomile tea is consumed more than a million cups a day worldwide. The beneficial
effects of chamomile on health, determined to have more than 120 compounds, are generally divided
into two classes: sesquiterpenic compounds such as a-bisabolol, bisabololoxides A and B,
chamazulene and farnesene, andphenolic compounds, namely flavonoids including apigenin,
quercetin, patuletin and luteolin, and their glucosides. In addition, coumarins are also considered to be
an important bioactive component. Studies conducted so far have shown its antiparasitic, antioxidant
and anticancer characteristics which support the use of the chamomile in the treatment of various
diseases. Chamomile has been used to treat a variety of inflammation, irritation, and pain such as skin
diseases, sores, eczema, ulcers, gout, neuralgia, and rheumatic pain. It has been determined in animal
model studies that chamomile has strong anti-inflammatory effect, and antispasmotic and anxiolytic
effects as well as some antimutagenic and cholesterol-lowering activities. Recent studies have focused
on the anti-diabetic effects of chamomile tea. The purpose of this study is to investigate the effects of
various bioactive compounds found in the content of chamomile tea.

KEYWORDS
Chamomile tea, health, medicinal plant, antioxidant

Corresponding Author: JYAN ASLAN, jynaslan@gmail.com

1
ANKARA NVERSTES SALIK BLMLER FAKLTES

1150
 Poster Session 8
Submission ID: 1086

CURE COMES FROM THE WATER CHLORELLA AND SPIRULINA

1 1 1 1 1
ASENA NVER , SEVM HAMZAEB , DLCAN AKPINAR , FATMA ZTRK , HATCE GNDZ

ABSTRACT
The need for new food supplies has arisen due to the increase in the population of the world.
As the terrestrial sources are unable to cope with the increasing demand people have a tendency
towards aquatic resources. One of the most important aquatic creatures is the algae. In addition to their
being a nutrient, they are also used as medicine. Their importance comes not only from the fact that
they are the nutritional source of a variaty of aquatic creatures but also from their part in producing the
two thirds of the worlds photosynhate carbon and their function in maintaining the integrity of the
ecosystem. As a nutrition and a medicine algae has a major importance in China, Japan and Korea. In
many countries it is used in various fields such as medicine, pharmacology, cosmetics, chemistry,
agriculture, food industry and energy sector Chlorella and Spirulina are the two most prominent algae
species used in food supplement industry in our country as well as the rest of the world. If the fossil
records are to be examined it could be noticed that Chlorella has a cell structure that has not been
changed for almost 2.5 billion years.. Spirulina has become widely used throughout the world thanks
to its being an essential food of the Aztecs. Spirulina is a protein source with its low level of fat and
calories and it contains nearly all essential amino acids. These two species of seaweed are prefered due
to their rich nutritional value and that they can be reproduced in labrotaries. These algae species are
rich in vitamin B12, minerals, protein and iron and that makes them useful in the renewal of the heart
cells and in fighting against diseases such as high levels of cholestrol, ulcer, cancer,flu etc. In Turkey,
this two of algae are sold by many of companies as a food supplement.

KEYWORDS
Spirulina, Chlorella, algae, treatment, food

Corresponding Author: ASENA NVER, asenagulata@hotmail.com

1
ZMR KATP ELEB NVERSTES

1151
 Poster Session 8
Submission ID: 1087

EFFECTS OF COMMON DAISY (BELLIS PERENNIS) EXTRACT ON

EXPERIMENTAL TOXOPLASMOSIS

1 1 1 1 1
FATMA PEHLIVAN KARAKA , EROL AYAZ , KEREM YAMAN , AYHAN ETINKAYA , MCAHIT AKMAK ,
1
GKE BOZAT

ABSTRACT
Effects of Common Daisy (Bellis perennis) Extract on Experimental Toxoplasmosis Erol
AYAZ1, Fatma PEHLVAN KARAKA2, Kerem YAMAN1, Ayhan ETNKAYA3, Mcahit
AKMAK4, Gke BOZAT2 1 AB Medical Faculty, Department of Parasitology, BOLU 2 AB
Agriculture and Natural Sciences Faculty, Division of Medical and Aromatic Plants, BOLU 3 AB
Medical Faculty, Department of Physiology, BOLU 4 AB Lab Animals Application and Research
Center, BOLU Aim of our study is to examine the anti-parasitic effects of Bellis perennis extract on
the experimental model of toxoplasmosis. Bellis perennis is a herbal perennial plant used as an
antimicrobial and anti-inflamatory agent in traditional medicine. Some researchs showed the diuretic,
analgesic and anti-pyretic effects of this plant, especially the leaves. Toxoplasma gondii is an obligate,
intracellular parasite which has got a high seropositivity around world. Although toxoplasmosis is not
a clinically symptomatic infection, it gains importance at immunocomprimised patients and fetuses.
Dependent to the anti-microbial, anti-inflammatory effects, there is no research on the usage of
B.perennis as an anti-parasitic agent. Method: We used ethyl acetate evaporated and lyophilised
essence of B.perennis, We applicate this extract to 40 mice aged 2-4 months old in this study. Mice
were divided into five groups according to their situation of infection and intragastric application of
extract. All mice in chosen groups were infected with 1X105 tachyzoites of T.gondii RH strain. As an
antiparasitic agent, 24 mg/ml dosage of co-trimoxsazole in saline, is chosen and dispensed 0.1 ml by
oral gavage . Dosage of B.perennis extract was adjusted 100 mg/kg in saline and dispensed 0.1 ml by
oral gavage too. Group I was the healthy group without any intervention. Group II was infected with
T. gondii tachyzoites. Group III was infected and treated with co-trimoxsazole. Group IV was infected
and treated with B.perennis extract. Last group was the group, we both apply the plant extract and co-
trimoxsazole. After, chosen groups were infected, we observed the groups and examined the parasite
load by counting tachyzoites in the intraperitoneal fluid of mice at Thoma chamber. Results:
According to parasite load of study groups, we found significant decrease of tachyzoite levels in the
groups III, IV and V, within comparison to control group II. Besides, no significant difference is
indicated between groups III, IV and V. All mice but groups I and II, are found dead on day 7 post-
infection. There is no change in normal life span of mice in group I and all mice in control group II are
found dead on day 2 and 3 post-infection. As a result of our study, we concluded that the ethyl acetate
extract of B.perennis is found beneficial for toxoplasmosis. We advise the usage of this herbal extract
as an anti-parasitic agent could be helpful to cure toxoplasmosis.

KEYWORDS

Corresponding Author: KEREM YAMAN, keremyamantbb@yahoo.com.tr

1
ABANT ZZET BAYSAL NVERSTES

1152
Toxoplasma gondii, Bellis perennis, Mice, Anti-parasitic

1153
 Poster Session 8
Submission ID: 1088

A FRUIT PACKED OF HEALTH: TAMARIND (TAMARINDUS

INDICA)

1 1 1
MUSTAFA FEVZI KARAGZ , MAKBULE GEZMEN-KARADA , HILAL YILDIRAN

ABSTRACT
Tamarind belongs to the family of Fabaceae and is one of the fruits used in ayurvedic
medicine. This fruit, rooted in Africa, is then exported to Asian countries such as India and Indonesia,
and to America countries such as Mexico and Costa Rica. Tamarind is rich in pyhtochemicals besides
vitamin and mineral content. Tamarind leaves have antiinflammatory features that diclofenac
(NSAID)-like effect. In diabetic rats, using tamarind seed extract by 120 and 240 mg/kg per day, the
levels of NO were decreased by 22 and 43%, respectively. When compared to corresponding diabetic
control rats, TNF- levels were dose-dependent decreased, dramatically (p<0,05). Tamarind has
antidiabetic properties due to increasing pancreatic intracellular Ca+ levels by inslin-like action in
pancreatic cells. Tamarind shows laxative activity because of fiber, malic and tartaric acid content,
and also its bark and leaves are used for wound healing. Due to acidic content longed-term use can
lead to tooth decay. The content of tannin can make it difficult to digest tamarind, so it is
recommended to consume it as boiled or in water. When tamarind consume with asprin and ibuprofen,
bioavailability of and blood concentration of drugs increase. Considering health effects such as
antiinflammatory, antioxidant, antidiabetics etc.,be careful for drug interactions and other potential
adverse effects in the use of tamarind which is inevitable in the use of ayurvedic medicine.

KEYWORDS
Tamarind, Tamarindus indica, Antiinflammatory, Antioxidant

Corresponding Author: MUSTAFA FEVZ KARAGZ, mmufeka@gmail.com

1
GAZ NVERSTES SALIK BLMLER FAKLTES BESLENME VE DYETETK BLM

1154
 Poster Session 8
Submission ID: 1089

USE OF HAWTHORN (CRATAEGUS SPP.) FOR DIFFERENT

MEDICINAL USES

1 2
MURAT AYDEMIR , MEHMET NURI NAS

ABSTRACT
The hawthorn is known in Turkey with different names such as yemisen, alic, aluc, erderan,
beyaz diken or eksi musmula. There are up to 200 species of recipient of the Rosaceae family under
the Crataegus genus in the world, and this number has been increased to 1200 by some taxonomists.
There are 17 species of Crataegus in Turkey. Naturally the most spreading species is Crataegus
monogyna at the same time Crataegus orientalis, Crataegus oxyacantha and Crataegus aronia species
are also common. Hawthorn fruit and flowers contain many useful substances for human health,
especially antioxidant flavonoids (flavanes), vitamins (especially vitamin C), saponins, organic acids,
ether oils and sugars. In the field of medicine, the number of studies analyzing the effects of
substances contained in hawthorn fruits on human health are increasing day by day.

KEYWORDS
Hawthorn, medicine, antioxidant

Corresponding Author: MURAT AYDEMR, murataydemir46@gmail.com

1
KAHRAMANMARA ST MAM NV, TRKOLU M.Y.O.
2
IRNAK NV. ZRAAT FAK. BAHE BTKLER BLM

1155
 Poster Session 8
Submission ID: 1090

NUTRITIVE VALUE AND FUNCTIONAL PROPERTIES OF CHIA

SEED (SALVIA HISPANICA L.)

1 1 1 1
YELIZ SERIN , GAMZE AKBULUT , NILFER ACAR TEK , HATICE BLKBAI

ABSTRACT
Salvia hispanica L., also known as chia, is a herbaceous plant cultivated semi-annually, and it
belongs to the family Labiatae, division Spermatophyta, and kingdom Plantae. Chia is native to the
region that stretches from North Mexico to Guatemala. Its seeds were widely used by Aztec tribes for
food, medicine, and paints. Nowadays chia seeds are commercially grown in Mexico, Bolivia,
Argentina, Ecuador, and Guatemala. The seeds are small with an oval, flattened shape and ranged in
color from dark coffee to beige with small darker spots. The chia plant is sensitive to daylight and
produces black and white seeds. Black colored chia seeds are more common. White seeds are larger,
thicker and broader than the black seeds.The plant can grow in a wide range of well-drained clay and
sandy soils with reasonable salt and acid tolerance.Different ecosystems have variable significant
effects on the nutrient composition of Salvia hispanica especially its protein and oil content and also
the fatty acid composition. The environmental factors which have been found to influence the
composition of chia seeds include temperature, light, soil composition and type/variety. Chia seed
contains a significant amount of lipids (approximately 40% of the total weight), with almost 60% of
the lipids comprising Omega-3 fatty acids. Dietary fiber constitutes more than 30% of the total weight
of the seed, and approximately 19% of the seed contains proteins of high biological value. The 25-g
serving of chia seeds had 540 kJ, with 7.7 g total fat, 3.9 g protein, 11.0 g carbohydrate, 9.4 g dietary
fiber, 4.4 g ALA, 1.4 g linoleic acid, and 158 mg calcium. Chia seeds possess many important
functional properties(water-holding capacity (the chia seeds can absorb water up to 12 times their
weight), oil holding capacity, solubility, viscosity, emulsion stability and foaming stability) which
prove its potential to be used as a thickening agent, gel forming agent, chelator, foam enhancer,
emulsifying agent, clarifying agent, rehydrating agent and as suspension formers in the formulation of
food products at both home and commercial level. All these properties make chia a promising
functional food for the future.From the past to the present day, possible therapeutic effects of chia
(such as control of diabetes, dyslipidemia, hypertension, as anti- inflammatory, antioxidant, anti-blood
clotting, laxative, antidepressant, anti-anxiety, analgesic, and immune improver) has been discussed in
the scientific literature. Although not common in studies on humans, some research points to the
consumption of chia having a positive effect on health.However, these studies vary a great deal in both
sample size and the profiles of the individuals in the sample group, which may have created a
discrepancy in the findings. Furthermore, the quantities and forms of chia seed ie, milled, whole-
seed, baked in bread were different in the different studies. Also, studies of chias intake in a human
diet which take into consideration factors such as lifestyle and genetic variations are still limited.
European Parliament and Council of Europe approved chia seeds as a Novel Food in 2009. Till date,
no study has revealed any adverse toxic, allergic or anti-nutritional effects after ingesting whole or
ground chia seeds. In 2015, Garca Jimnez S and co-workers described the first case of an IgE-

Corresponding Author: YELZ SERN, dytyelizserin@gmail.com

1
GAZ NVERSTES, SALIK BLMLER FAKLTES, BESLENME VE DYETETK BLM

1156
mediated anaphylactic reaction induced by chia seeds. The allergens involved are water-soluble and
liposoluble and include a lectin, an elongation factor, and an 11S globulin as known allergens. So,
more clinical trials on animals and humans need to be done to ascertain the safety aspects of chia
seeds.

KEYWORDS
chia, nutrient, health, functional properties

1157
 Poster Session 8
Submission ID: 1091

USE OF MICROELGAN'S AS PIGMENT SOURCE

1 1 1 1 1
DLCAN AKPINAR , ASENA ASENA NVER , SEVM HAMZAEB , FATMA ZTRK , HATCE GNDZ

ABSTRACT
Microalgae, which are abundant in nature, contain a wide variety of color materials in their
structures. Some of those; Astaxanthin, beta-carotene, phycocyanin, xanthophyll and phytoerythrin.
These pigments can be used as natural pigments in food, medicine, textiles and cosmetics instead of
synthetic pigments thought to be carcinogenic. Phycocyanin is a natural coloring substance with strong
fluorescence properties, blue color, odorless, non-toxic, water-soluble. Due to its fluorescent
properties, it is used as a fluorescent probe in microscopic, cytometry, immunology, tissue chemistry
studies. In addition, they are used in nutraceutical and pharmaceutical applications due to antioxidant
and radical scavenging effects. In this review, the pigments obtained from microalgae, their uses and
new technologies will be mentioned.

KEYWORDS
Microalgae, Phycocyanin, Carotene, Pigment

Corresponding Author: DLCAN AKPINAR, mdilcanakpinar@gmail.com

1
IZMIR KATIP ELEB NVERSTES

1158
 Poster Session 8
Submission ID: 1092

DETERMINATION OF ANTIOXIDANT AND ANTIMICROBIAL

ACTIVITIES OF FIVE MULTIFLORAL HONEY SAMPLES FROM
COLLECTED DIFFERENT REGIONS IN TURKEY

1 2 1 1 1
DUYGU ODABA ALVER , MER ERTRK , LGIN KIZILPINAR TEMZER , AYTA GDER , SEVIM FT YEGN

ABSTRACT
Honey possesses antioxidant and antimicrobial activities. Many chronic diseases are
associated with increased oxidative stress caused by an imbalance between free-radical production and
antioxidant level. For that purpose, the total phenolic contents, antioxidant potentials and antimicrobial
activities of five multifloral honey samples obtained from Turkey were investigated. The world
population living in rural areas have confidence in herbal traditional medicines as their primary health
care, the work on features and uses of medicinal plants and honey are getting growing curiosity. The
difference between them indicated the presence of antimicrobial substance in honey. The kinds of
antimicrobial substances (inhibines) in honey are evaluated. Hydrogen peroxide is not the only
inhibine in honey. In fact, inhibines in honey include many other substances. Two important classes of
these inhibines are the flavonoids and the phenolic acids. In this study, five multifloral honeys were
compared with using pollen analysis, different antioxidant and antimicrobial test methods. The
antioxidant capacity of honeys was assessed through the hydrogen peroxide scavenging activity (in
terms of SC50), ferric reducing antioxidant power capacity (FRAP), DPPH radical scavenging activity
(in terms of SC50), metal-chelating activity (%), total phenol content (TPC), and total flavonoid
content (TFC). Additionally, antimicrobial activities of honey samples were investigated by using disc
diffusion assay method against four Gram positive bacterias (B. subtillis, S. aureus, L.
monocytogenes, C. perfringens), four Gram negative bacterias (P. aeruginosa, E. coli, S. enteritidis, K.
pneumoniae) and a fungus (C. albicans). The antioxidant capacities of multifloral honeys were found
between 251.99.-269.96 g/mL(HPSA), 72.47-74.66% (FRAP), 599.95-633.75 g/mL(DPPH), 34.45-
72.90% (MCA), 26.66-112.8 mg GAE/100 g (TPC) and 5.39-9.32 mg CAE/100 g (TFC). For
comparison of these results, buthylated hydroxy anisole (BHA), buthylated hydroxy toluene (BHT)
and -tocopherol (TOC) were used as standard antioxidant compounds. The antimicrobial activity of
multifloral honeys were found against B. subtillis, S. aureus, L. monocytogenes, C. perfringens, P.
aeruginosa, E. coli, S. enteritidis, K. pneumoniae, C. albicans between 9.78-14.44, 6.00-13.11, 8.64-
13.12, 6.00-12.27, 10.32-14.10, 6.00-15.22, 9.78-13.85, 6.00-13.22 and 6.00 mm, respectively. As a
result, it has been observed that multiflower honeys had antibacterial effects against the Gram (+) and
Gram (-) bacteria activities but not the fungus. The result of this study indicates that antimicrobial and
antioxidant activities of multifloral honeys can be varied based on pollen composition.

KEYWORDS

Corresponding Author: DUYGU ODABA ALVER, duygu.odabas@giresun.edu.tr

1
GRESUN NVERSTES
2
ORDU NVERSTES

1159
antioxidant, antimicrobial, multifloral honey, pollen composition

1160
 Poster Session 8
Submission ID: 1093

KONJAC GLUCOMANNAN AND HEALT BENEFITS

1 1 1 1
MUSA SERDAR AKIN , KAMILE BURCU BYKKILI , ASLI ELKEL , MUTLU BUKET AKIN

ABSTRACT
The konjac glucomannan (KGM) is a water-soluble polysaccharide (dietary fibre) isolated
from tubers of Amorphophallus konjac K. Koch. It is a perennial plant belonging to the family
Araceae. Amorphophallus konjac K. Koch has been cultivated for centuries in Asian countries as a
source of food and as an ingredient for traditional Chinese medicine. Konjac products are regarded as
one of the top 10 health foodsby World Health Organization. The polysaccharide has been largely
consumed as a nutritional supplement. The important health benefits of KGM includes in reducing
cholesterol, normalizing triglyceride concentration in blood, promoting intestinal activity and immune
function, improving blood sugar levels and wound dressing. KGM is considered as an indigestible
dietary fibre being resistant to hydrolysis by the action of digestive enzymes in the human gut. In
pharmaceutical industry, KGM is used in the preparation of hydrogel as a DNA-controlled release
matrix. In addition, it has been used to improve glycaemia and other related risk factors for coronary
heart diseases in Type II diabetic patients. Therefore, KGM is recognized as a safe biomaterial
according to the FDA (Food and Drug Administration,USA) for therapeutic uses. In this review,
health benefits of KGM have been investigated.

KEYWORDS
Konjac glucomannan, dietary fibre, health benefits

Corresponding Author: MUSA SERDAR AKIN, sakin@harran.edu.tr

1
HARRAN NVERSTES

1161
 Poster Session 8
Submission ID: 1094

MINERAL AND FATTY ACID COMPOSITION OF NIGELLA SATIVA

OIL

1 1 1 1
SERPIL KILI , TIMUR TONGUR , MURAT KILI , TANER ERKAYMAZ

ABSTRACT
Nigella sativa, is a widely distributed annual herbaceous plant. For many years, the plants us
efor protective and terapeutic purposes from diseases and the usage are increased for lesser side effects
and more safe of plants. These kind of products are increased depends on these interests. The efficacy,
stability and quality of these products are affected to the public health. In particular, Nigella sativa oil
is considered as one among newer sources of edible oils and it is important role in human nutrition and
health. Nigella sativa oil or extract has protective and curative actions. Therefore, this investigation
was undertaken to obtain information about the mineral composition of cold-pressed Nigella sativa oil
sample and to the determine fatty acid profiles. In this study, the concentrations of mineral
composition (Cu, Fe, Mg, Mn, Se and Zn) were determined in Nigella sativa oil by inductively
coupled plasma mass spectrometry (ICP-MS). The samples were digested with nitric acid and
hydrogen peroxide in a microwave oven. The calibration was accomplished by adding the standards
prepared in the concentrations of 2, 5, 10, 25, 50, 100 and 200 g L1 from the 10 mg L1 stock
standard solution. In sample levels of Cu, Fe, Mg, Mn and Zn were found to be 475 g/L, 20 mg/L,
112 mg/L, 456 g/L, 10 mg/L, respectively. Selenium could not be detected. Determination of fatty
acid and the esters profiles were completed by using gas chromatography mass spectrometry (GC-
MS). Prepared samples, for FAME analysis, a TRACE TR-Wax GC capillary column (30 m 0.32
mm) was used. The gas chromatograph system was combined with Thermo Trace GC and ISQ mass
selective detector. The GC oven was kept at 60 C for 3 min, heated at 8 C/min up to 300 C, where it
was kept for 1 min, and a total analytical time was 34 min. The carrier gas was helium (1 ml/min). The
analysis of a sample by GC was carried out by injecting 2 l of the sample solution into the GC. The
formed methyl ester was identified by a selected ion monitoring (SIM) method. The major
components of fatty acids in Nigella sativa oil are linoleic (%41), palmitic (%24) and oleic acids
(%17). % Levels of Myristic acid, Palmitoleic acid, Heptadecanoic acid, gamma-Linolenic acid
(GLA), Elaidic acid, Stearic acid, dihomo--linolenic acid, Methyl Eicosenoate, cis-11,14-
Eicosadienoic acid, Arachidic acid and Behenic acid were found to be 0.6, 0.2, 0.1, 2.3, 0.7, 9.5, 0.7,
0.5, 2.2, 0.4 and 0.1, respectively.

KEYWORDS
Nigella sativa oil, essential oil component, GC-MS, mineral analysis and ICP-MS.

Corresponding Author: SERPL KILI, serpilkilic@akdeniz.edu.tr

1
AKDENZ NVERSTES GIDA GVENL VE TARIMSAL ARATIRMALAR MERKEZ

1162
 Poster Session 8
Submission ID: 1096

PSEUDO-CEREALS: BUCKWHEAT AND QUINOA

1 1 1 1
HILAL BETL ALTINTA , YELIZ SERIN , GAMZE AKBULUT , NILFER ACAR TEK

ABSTRACT
Cereals such as teff and millet, which grows rarely and/or in specific places, are called
minor-cereals; small-pieced cereals, which are seed-sized, are called pseudo-cereals. Botanically
speaking, most cereals such as wheat, barley, and rye are monocots while pseudocereals such as
amaranth, quinoa, and buckwheat belong to eudicots group. Buckwheat Buckwheat is an annual plant
which belongs to Polygonaceae family. It is shown with 20-25 cm intervals at the end of March or at
the beginning of April and harvested in September or October. The height of buckwheat to be
harvested can change between 60-120 cm according to conditions and climate type. One of the most
important features of buckwheat is that it can adapt to growing at high altitudes in a short time.
Buckwheat grain of which glumes are removed are called groat. Those are the ones which havent
gone through heat process; raw grains of buckwheat. Groats, fractured or whole, have no inedible
black glue on. Unprocessed white grains are a bit bitter. For his reason, if they are roasted with some
oil until they turn rustish red before cooking, this bitter taste will disappear and leave a nice flavor.
Most common buckwheat types grown as a food source are common buckwheat (Fagopyrum
esculentum Moench) and Tatar Buckwheat (Tartary Buckwheat-Fagopyrum tataricum Gaerth).
Common buckwheat is usually preferred because it has a delicious taste and a big seed, while Tatar
buckwheat is preferred less because it has a bitter taste and a glue which can be peeled difficultly. The
color of buckwheat is among the quality criteria along with its taste. Freshly harvested buckwheat
seeds are light green. The color of old seeds is reddish brown. Whereas the annual buckwheat
production changes year to year worldwide, the average production is around 3.5 million ton at
present. In last 40 years, China has been the biggest buckwheat-producing country. %73.5 of a
buckwheat grain consists of starch and %33.5 of it consists of resistant starch. Glycemic index of
nutrients which contains resistant starch are usually low and this condition is an advantage for healthy
adults. Quinoa Quinoa (Chenopodium quinoa Willd), an annual plant, is a member of goosefoot
family (Chenopodiaceae) which includes spinach and beet. This plant is an endemic plant which
generally belongs to And Region of South America (Colombia, Argentina, Peru, Bolivia, Chile, and
Ecuador). Moreover, it is resistant to unsuitable climate conditions (frost, drought, etc.) and soil
conditions (such as saltiness). Quinoa, used as a traditional food by most South American natives, like
rice, can be used as a pilaf or be putting in soup dishes. Also, quinoa flour can be used for production
of bakery products such as cookies, bread, biscuits, crisps, and pancakes. Leaves of this plant can be
consumed like spinach and its sprouts can be added to salads. Buckwheat and quinoa are commonly
used especially in the gluten-free food industry. Buckwheat (Fagopyrum esculentum Moench)
includes a high level of protein, diet fiber, vitamins, minerals, basic non-saturated fat acids and
antioxidants such as rutin and quercetin. Likewise, quinoa includes important micro-nutritional
compounds such as minerals, vitamins and bio-active elements in balanced amounts.

Corresponding Author: YELZ SERN, dytyelizserin@gmail.com

1
GAZ NVERSTES, SALIK BLMLER FAKLTES, BESLENME VE DYETETK BLM

1163
KEYWORDS
psedo-cereal, buckwheat, quinoa, grain

1164
 Poster Session 8
Submission ID: 1097

FUNCTIONAL FOOD AWARENESS OF VOCATIONAL HIGH

SCHOOL STUDENTS

1 1 1 1 1
NURHAN GMRKOLU , DIDEM SARIMEHMET , SEVILAY HINTISTAN , BAHAR CANDA , GONCA SERDAR

ABSTRACT
Functional food; Is food or food components that reduce or mitigate some of the disease and
health conditions as well as basic nutrition. In recent years, globalization, population growth, changing
living standards with urbanization, increased health spending, increased elderly populations, and the
emergence of various diseases have caused changes in the diet of individuals. Individuals are trying to
take precautions by using health protective natural products as well as medicinal products such as
medicines for the protection of diseases for healthy and quality life. The aim of this study is to
determine the factors affecting the attitudes, knowledge levels and functional power consumption of
the students attending to KTU Health Services Vocational School. A questionnaire consisting of
various questions was applied to the students under the 7 sections heading. Within the scope of the
research, the students were evaluated whether they were familiar with the term functional foods,
consumed these nutrients, consumed them and their reasons, level of knowledge about health claims,
in which situations they wanted to consume these foods and how they wanted to be informed.
According to the results of the research, it has been determined that the three most functional foods
consumed are mineral water (%76,8), herbal teas (%66,3) and Milk and dairy products that help
digestion (%49,5). The aim of students to use functional foods for their favorite taste (%56,8), the
second in order to be healthy (40.5%), in third place to assist in the digestion was determined as
(%33.2). It has been determined that the least use is to reduce the risk of high blood pressure. As a
result of the statistical analysis, it was seen that there was no meaningful difference between genders
in terms of sources of information. In this study it was concluded that students did not know that
functional foods were named as functional foods despite their definitions.

KEYWORDS
Student, Functional food, Awareness, Health

Corresponding Author: NURHAN GMRKOLU, ngumrukcuoglu@ktu.edu.tr

1
KARADENZ TEKNK NVERSTES

1165
 Poster Session 8
Submission ID: 1099

NATURAL MUSHROOMS CONSUMED IN TURKEY AND THEIR

CONSUMPTION PATTERNS

1 1 1
YASIN UZUN , ABDULLAH KAYA , SEMIHA YAKAR

ABSTRACT
Introduction: Fungi are important organisms that serve many vital functions especially in
terrestrial ecosystems. Thoose fungi producing large and easily observed fruiting bodies are referred as
macrofungi. The best known examples of macrofungi are the mushrooms. Wild edible fungi are
collected and consumed by people for thousands of years. There is a huge diversity of different types,
from truffles to milk-caps, chanterelles to boletes. Current checklists and the contributory studies on
Turkish higher fungi were traced and it is found that about 300 edible taxa belonging to 9 orders, 40
families and 72 genera have so far been identified in Turkey and almost 54 species of them are
generally or locally collected and consumed or marketted. Compared to edible macromycota of Tukey,
naturally growing mushroom consumption can be regarded as very low. The Turkish people are
especially know; Morchella esculenta as Kuzu Gbei; Lactarius deliciosus as Kanlca Mantar,
intar ; Terfezia boudieri as Dolaman, Keme; Agaricus campestris as ayr Mantar ; Pleurotus
ostreatus as stiridye Mantar, Kavak Mantar, Kayn Mantar; Boletus edulis as Ay Mantar ;
Cantharellus cibarius as Tavuk Mantar; Craterellus cornucopioides as Borazan Mantar, Huni
Mantar and Helvella leucomelaena as ukur anak Mantar. Some of the edible fungi especially
Agaricus bisporus, Pleurotus ostreatus, Pleurotus citrinopileatus, Ganoderma lucidum, Lentinula
edodes, Grifola frondosa, Hericium erinaceus and Flammulina velutipes are also cultivated in Turkey.

KEYWORDS
Wild edible macrofungi, mushroom consumption, Turkey.

Corresponding Author: YASN UZUN, yasinuzun_61@hotmail.com

1
KARAMANOLU MEHMETBEY NVERSTES

1166
 Poster Session 8
Submission ID: 1100

COMPARATIVE OF THEIR FUNCTIONAL PROPERTIES BY MIXING

VEGETABLE OIL IN THE DIFFERENT RATIO

1 2 2
EYMA ETINER , BLENT BAYIIT , BRAHIM HAYOLU

ABSTRACT
Vegetable oils have an important role to regulate the body's functions. Vegetable oil prevent
lots of health problem. They relax body as mental and physical. Vegetable oils are more preferred than
animal oils because it is thought that animal oils have some health problems. Vegetable oils have low
ratio of saturated oil, contain oil acids which is necessary for body and they can solve Fat-soluble
vitamin as A, D, E, K. Vegetable oils have high nutrition values. The objective of this study obtained a
functional product by mixing oil of sesame, soybean, mustard and cumin. Antioxidant capacity of
body is fungible when people consume this oil mixture because they contain vitamin e, variety sterols
and sesamin, sesamol. Average refractive index, L*, a*, b*, peroxide and free acidity of oils were
found to be between 1.47-1.48, 21.88-26.85, -0.15-3.68, 7.10-12.62, 6-6.25 meqg 02 kg-1 ve 0.56-3.86
(as oleic acid).

KEYWORDS
sesame, soybean, mustard, cumin, oil

Corresponding Author: EYMA ETNER, seyma_cetiner27@hotmail.com

1
2
HARRAN NVERSTES MHENDSLK FAKLTES GIDA MHENDSL BLM

1167
 Poster Session 8
Submission ID: 1101

UZERLIK (PEGANUM HARMALA L.)

1 1 1
EKIN DNEL , AYLA NVER ALAY , MERYEM BADAYMAN

ABSTRACT
From past to present day plants are used in the treatment of many diseases. In addition to this,
it is known that a variety of plants have been used for the purpose of protection from the whammy.
Very commonly known as "Peganum harmala" is a plant species from the family Nitrariaceae that
grows in the hot regions of Africa, Asia and America. In our country, it is found in all Central
Anatolian steams, especially in Ankara, Konya, Sivas, Nigde, Erzincan, Kayseri and anliurfa. This
plant is also known as "uzerlik, nazar otu, wild pearl grass, sipend, cypendan, isfend, mahmurcicegi,
yellow garlic, ilezik, eldruk and ilruk". Uzerlik (Peganum harmala) is a perennial plant that blooms in
May-July, with white flowers, 30-50 cm in length. The green cone-shaped fruit has brown seeds
inside. The fruit is a flat globular capsule. Once the seeds of the uzerlik (Peganum harmala) are dried,
the tea can be made. It can be consumed by mixing with honey and baked or mixed with water.
Moreover, the amulets made from the Peganum harmala are hanged in the houses. In order to prevent
hazelnut, grains of Peganum harmala or incense of this seed are widely used in Anatolia. The active
components of the uzerlik are alkaloids, which are especially found in seeds and roots. The total
alkaloid ratio is 4-7%. Among the alkaloids harmin, harmaline, harmol and blend are important. There
are also glycosides called peganine and red dye in their seeds. This paint material is used for dyeing
Turkish carpets. The alkaloids obtained from P. harmala seeds have a broad spectrum of
pharmacological action. The seeds have hypothermic, hallucinogenic, antibacterial, antifungal,
antiviral, antitumoral, vasorelaxan and analgesic effects. P. harmala extract is toxic at high-doses and
can cause digestive problems (nausea, vomiting), liver degeneration, kidney lesions, paralysis,
spongiform changes in the central nervous system, euphoria, convulsions, hypothermia and
bradycardia. In high doses of intoxication, it can be fatal. However, therapeutic doses have been
reported to be safe in some countries (e.g. Morocco), this plant has been reported to have been used at
high doses for pregnancy termination rather than illegal abortion. Quinazoline alkaloids (e.g., vasicine
and vasicinone), within P. harmala, have been attributed to the abortifacient effect of this plant. This
study reviews the traditional uses and pharmacological effects of total extract and individual active
alkaloids of P. harmala.

KEYWORDS
P. harmala, uzerlik, whammy grass, benefits, damages

Corresponding Author: EKN DNEL, ekindincel@aydin.edu.tr

1
STANBUL AYDIN NVERSTES

1168
 Poster Session 8
Submission ID: 1104

CIRIS (ASPHODELUS AESTIVUS L.)

1 1 1
MERYEM BADAYMAN , AYLA NVER ALAY , EKIN DNEL

ABSTRACT
Despite the fact that nearly 10,000 plants pecies grow naturally in our country, they are not
utilized sufficiently. In order to benefit from Turkey's rich plant diversity, it is necessary to collect
information on the use of plants in folk medicine cultures, to identify and separate chemical and
biological methods and their active ingredients from natural sources, and to develop medicines for the
treatment of diseases. Among the Asphodelaceae family, Asphodelusaestivus L. which is the genus of
Asphodelus genus, is known among the population as Asphodelusaestivus. The leaves of the
Asphodelus aestivus plant are similar to the leek leaf; However, it is rather small compared to leek and
in most are as it is called "wildleek, Gllk, Yeling, grass and yellowlily". Asphodelusaestivus L.
(Liliaceae), 50-150 cm in length, leaves in the form of swords, 35-45 cm in height, 3 cm in width; It is
a gren plant all year round with pea-shaped gren fruits, 7 mm in size, flowering in April-May. It grows
mainly in dry, poorly nutritious, sandy, non-cultivated soil. It is also used as an ornamental plant. It is
a perennial plant that is found in some parts of Africa, Arab countries, Musar, Turkey and Europe.
Asphodellus aestivus has been used as a foodstuff and traditionally because of its diuretic, wound
healing, antihemoroidal, menstrual facilitating activities and therapeutic properties on alopecia and
abscess. It is used as food for roots, flowering hulls and seeds, and also leaves and canned food. This
plant grows in the high mountains in the spring in our country and the green leaves that come out of
the soil are cut and sold as vegetables. Many dishes such as boiled, soup, salad, baked, yahnisi, rice
and pie are consumed frequently during the season. There is also the use of dried grass in powder form
by drying and grinding. There is a unique smell. Using this feature, the leaves of the plant are used in
the production of Italian cheese "rignano garganico" and in the production of herbed cheese.
Asphodels apart from these yeast industry, In Erzurum, ehram fabric is used to give firmness and
shine. Many investigations have reported that Asphodelusters contain valuable chemical compounds
such as antranoids, flavonoids, steroids, triterpenes and arylcoumarins, anthraquinones and glycosides,
and these gastroprotective, antimicrobial, antifungal, antioxidant, cytotoxic and apoptotic effects. It
has been proven that the Asphodel increases the white blood cells (WBC) and thus has an important
feature for our defense system. The root extract of AsphodelusaestivusBrot has a potential acaricidal
activity, and its use is thought to reduce unwanted side effects, health problems and environmental
pollution related to synthetic chemicals currently used in harmful management programs. Asphodelus
aestivus in Turkey is characterized by severe neuronal pigmentation with severe neural syndrome in
sheep.In this study, traditional uses and pharmacological effects of Asphadelus plant were
investigated. Key words: Asphodelusaestivus L, flavonoids. *stanbul Aydn University, ABMYO,
Food Technology Program, mbadayman@aydin.edu.tr, 4441428, Ext. 41803 1. Istanbul Aydn
University, ABMYO, Food Technology Program

Corresponding Author: MERYEM BADAYMAN, mbadayman@aydin.edu.tr

1
STANBUL AYDIN NVERSTES

1169
KEYWORDS
Asphodelus aestivus L., Flavonoids

1170
 Poster Session 8
Submission ID: 1105

DETERMINATION OF PLUMBAGIN PHENOLIC FROM PLUMBAGO

EUROPA BY HIGH PERFORMANCE LIQUID CHROMATOGRAPH
(HPLC)

1 2
EYYP KARAOUL , M. HAKKI ALMA

ABSTRACT
The aim of the present work was to determine the extractable plumbagin phenolic compounds
in the branches of Plumbago europa. The branches samples were obtained from Kahramanmaras
province of Turkey. The samples were extracted by using conventional extraction method for different
types of extraction and solvent. For this purpose, methanol, deionized water and city tap water were
preferred for solvent use. At the same time, the samples were extracted by boiling and without boiling
procedure at 100 oC for 30 min and maceration method during 1 day at the 25 oC with deionized
water and city tap water. The plumbagin phenolic in samples were determined as the concentration
both mg/kg (ppm) and percent amount by using high performance liquid chromatography (HPLC).
Moreover, it was found that the highest and lowest concentration of plumbagin in the Plumbago
europa were determined for methanol extraction (%54.09, 267mg/L) and maceration with city tap
water (%5.75, 11,96 mg/L), respectively. The results showed that methanol extraction has highest
concentration potential than other methods and solvents. Even, deionized water showed great potential
with boiling procedure in two methods than city tap water. Probably, inorganic compounds in city tap
water could be prevented the increase in extraction efficiency of plumbagin. Most likely also, because
of the increased penetration into the samples with boiling procedure, this method improved the
efficiency of plumbagin concentration.

KEYWORDS
Plumbago europa., plumbagin phenolic compounds, extraction methods, HPLC.

Corresponding Author: EYYP KARAOUL, e.karaogul@hotmail.com

1
HARRAN NVERSTES
2
KAHRAMANMARA ST MAM NVERSTES

1171
 Poster Session 8
Submission ID: 1106

SOME MEDICINAL PLANT AND THEIR HERBAL DRUGS

1 1
TUBA BAYRAK ZBUCAK , ZNUR ERGEN AKN

ABSTRACT
According to World Health Organization (WHO), Herbal medicines are drugs which prepared
from extracts or parts (root, leaf, flower, bark and seed) of plants for use in the treatment and to be
protected from diseases. Usage of plants for treatment begins with history of humanity. Today, many
drugs are derived from plants used in modern medicine. They are used as crude materials in the
pharmaceutical manufacturing industry because of that they contain active ingredients. In Turkey, the
plants used for medicinal is estimated to be around 500. The aim of this study was introduced some
medicinal plant and their herbal drugs. For this purpose, list of herbal origin drugs were provided from
pharmacies in our region. The most common 15 drugs of them were determined. In this study, general
features of medicinal plants and herbal drugs (commercially) made from them will be presented
together in a visual way. Some of them is Cinchona officinalis L. (Nuedexta), Centella asiatica L.
(Madecassol ointment), Cimicifuga racemosa L.(Remifemine), Aesculus hippocastanum L. (Venotrex
medicine), Cassia sp. (Sennalax medicine), Camellia sinensis (Veregen), Capsicum annuum L. (Algo-
wax Pomade), Hamamelis virginiana (Hametan ointment), Papaver sp. (Relistor), Atropa bellodana (
Sanctura).

KEYWORDS
Medicinal plant, Herbal Drug, Pharmacy, Turkey

Corresponding Author: TUBA BAYRAK ZBUCAK, tsiozbucak@hotmail.com

1
ORDU NVERSTES

1172
 Poster Session 8
Submission ID: 1109

EFFECTS OF ELLAGIC ACID ON EXPERIMENTAL

TOXOPLASMOSIS

1 1 1 2 1 1
EROL AYAZ , KEREM YAMAN , AYHAN ETINKAYA , ERSIN BEYAZIEK , MCAHIT AKMAK , GKE BOZAT

ABSTRACT
Effects of Ellagic Acid on Experimental Toxoplasmosis Erol AYAZ1, Kerem YAMAN1,
Ayhan ETNKAYA2, Ersin BEYAZEK3, Mcahit AKMAK4, Gke BOZAT5 1 AB
Medical Faculty, Department of Parasitology, BOLU 2 AB Medical Faculty, Department of
Physiology, BOLU 3 D Medical Faculty, Department of Physiology, DZCE 4 AB Lab Animals
Application and Research Center, BOLU 5 AB Agriculture and Natural Sciences Faculty, Division
of Medical and Aromatic Plants, BOLU Aim of our study is to examine the effects of ellagic acid on
toxoplasmosis. Ellagic acid is a natural phenolic anti-oxidant found in some fruits and vegetables,
especially the red ones like cranberries, raspberries, strawberries and pomegranate. These fruits make
ellagic acid via hydrolisis of tannins such as ellagitanin. Toxoplasma gondii is a worldwide spread
obligate and intracellular parasite which has got a high seropositivity level. Toxoplasma gondii causes
a disease called toxoplasmosis which is mostly asymptomatic. Depending to the immun system of the
host organism, it will cause some serious conditions, like toxoplasmic encephalitis. Ellagic acid is
used as an anti-parasitic against Plasmodium spp.; causative agent of malaria and Schistosoma species
but no study is done with T.gondii. Method: We used commercially sold ellagic acid dihydrate from
TCI company ( cat. No: E0375). We applicate this substance to 40 mice aged 2-4 months old in this
study. Mice were divided into five groups according to their situation of infection and intragastric
application of ellagic acid. All mice in chosen groups were infected with 1X105 tachyzoites of
T.gondii RH strain. As an antiparasitic agent, 24 mg/ml dosage of co-trimoxsazole in saline, is chosen
and dispensed 0.1 ml by oral gavage . Dosage of ellagic acid is adjusted 50 mg/kg in saline and
dispensed 0.1 ml by oral gavage too. Group I was the healthy group without any intervention. Group II
was infected with T. gondii tachyzoites. Group III was infected and treated with co-trimoxsazole.
Group IV was infected and treated with ellagic acid. Last group was the group, we both apply the acid
and co-trimoxsazole. After, chosen groups were infected, we observed the groups and examined the
parasite load by counting tachyzoites in the intraperitoneal fluid of mice at Thoma chamber. Results:
Related to parasite load obtained from study groups, we found significant decrease of parasite
numbers in the groups III, IV and V, within comparison to control group II. Also, no significant
difference is indicated between groups III, IV and V. Life span of mice is increased within comparison
to control. As a result, we advised that the ellagic acid is found an efficacious agent for toxoplasmosis
and may use for treatment. Further research is recommended for dosage optimization and different
ways of application.

KEYWORDS
Toxoplasma gondii, Ellagic Acid, Mice, Anti-parasitic

Corresponding Author: KEREM YAMAN, keremyamantbb@yahoo.com.tr

1
ABANT ZZET BAYSAL NVERSTES
2
DZCE NVERSTES

1173
 Poster Session 8
Submission ID: 1110

DETERMINATION OF TOTAL PHENOLIC CONTENT,

ANTIMICROBIAL, AND ANTIOXIDANT PROPERTIES OF
ESSENTIAL OIL AND EXTRACT OF THYMBRA SPICATA

1 1 1
EZGI METN , GULSUM UCAK , MUHAMMED ZEKI DURAK

ABSTRACT
Turkey is regarded as an important center for the family Lamiaceae (Labiatae). The family is
represented by 45 genera, 546 species and 730 taxa in Turkey. The rate of endemism in the family is
44.2%. The members of the Lamiaceae are mainly found in the mountainous areas of the
Mediterranean parts of Turkey. Thymbra spicata (Labiatae) is known as Zahter and Karaba
Kekik in the Southeastern Anatolia Region of Turkey. It grows wild in some eastern Mediterranean
countries. Their dried leaves are used as a condiment, herbal tea and folk medicine. It is known as
Thymbra spicata plant is used for diseases such as asthma, colic, coughs, bronchitis, rheumatism and
diarrhea. In this study, total phenolic content, antimicrobial, and antioxidant properties of essential oil
and extract obtained from Thymbra spicata medicinal plants were investigated as well as the efficacy
of two different extraction methods. The scope of study, two different methods were used, one of them
hydro distillated method for the essential oil and the other one methanol extraction method for extract
of the plant. The antimicrobial activity of the essential oil and extract of the plant against nine bacterial
strains (Bacillus subtilis, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853,
Salmonella typhimurium ATCC 14028, Listeria monocytogenes ATCC 13932, Klebsiella pneumoniae
ATCC 43816, Bacillus cereus ATCC 11778, E. coli O157 H7 ATCC 43888, Staphylococcus aureus
ATCC 25923) and one fungal strain (Candida albicans ATCC 10251) were researched using disc
diffusion method. The amounts of total phenolic compounds in the essential oil and plant extracts were
determined by the spectrophotometric Folin-Ciocalteu method. Total antioxidant capacities were
determined according to the DPPH method. Essential oils of Thymbra spicata are more effective than
extracts on all the tested bacteria. Essential oil of T. spicata showed the highest antimicrobial activity
against L. monocytogenes ATCC 13932 while the extract of T. spicata showed the highest
antimicrobial activity against Bacillus cereus ATCC 11778. The results of antioxidant activity showed
that the percentage of inhibition values ranged from 30.49 to 87.90 %. The oil of Thymbra spicata
showed that the lowest antioxidant capacity (30.49 %), while the extract of Thymbra spicata showed
the highest one (87.90%). It is found that the total phenolic content of the essential oil of Thymbra
spicata (2.70 mg/ml) is higher than the extract of plant (1.70 mg/ml). As a consequence, the data
obtained suggests that different extract method of T. spicata plant possesses useful antimicrobial and
antioxidant properties and may be utilized in the food industry and as a dietary supplement.

KEYWORDS
Thymbra spicata, Essential oil, Antimicrobial activity, Antioxidant activity, Total phenolic
content.

Corresponding Author: EZG METN, sevilezgi@windowslive.com

1
YLDZ TECHNCAL UNVERSTY

1174
 Poster Session 8
Submission ID: 1111

SOME HERBAL TEAS, USAGE AREAS AND PROPERTIES OF

PLANTS USED AS HERBAL TEA

1 1
TUBA ZBUCAK , ZNUR AKN

ABSTRACT
Herbal tea is beverage made from thein fusion or decoction of herbs, spices, fruits or other
plant material in hot water. Herbal teas are consumed cold or hot. Herbal teas are used in health and
beauty purposes in the world and in Turkey. In this study, fifteen plants consumed as herbal tea and
teas made from them will be discussed. Investigated plant and their teas are very popular and the most
used in Anatolia. These plants: Rosa canina L. (Rosaceae), Camellia sinensis (L.) Kuntze (Theaceae),
Salvia sp. (Lamiaceae), Tilia sp., Thymus sp. (Lamiaceae), Cinnamomum verumJ.Presl (Lauraceae),
Zingiber officinaleRoscae (Zingiberaceae), Melissa officinalis L. (Lamiaceae), Feniculum vulgare
Mill. (Apiaceae), Echinacea purpurea (L.) Moench (Asteraceae), Rosmarinus officinalis L.
(Lamiaceae), Hibiscus sabdariffa L. (Malvaceae), Mentha piperita L. (Lamiaceae), Matricaria
chamomilla L. (Asteraceae). Their morphological features, general information, parts used, methods of
using, names of tea, scientific name and Turkish name were determined. Herbal teas can be made with
fresh or dried flowers, leaves, seed sorroots, generally by pouring boiling water over the plant part
sand letting them steep for a few minutes. Green tea accelerates metabolism and also has antioxidant
properties. Linden tea is an antiperspirant and a breast emollient. Rosehip tea increases body
resistance. Sage tea opens appetite, facilitates digestion. Sage has antiseptic properties. For sores in the
throat, cold gargle is made. Thyme and cinnamon accelerate blood circulation. Cinnamon digestion
cures and has antioxidant properties. Rosemary strengthens the memory and increases concentration.
Echinacea tea is used for the treatment of diseases such as colds and flu. Matricaria chamomilla is
themost popular source of the herbal product chamomile. Chamomile tea can help to prevent and treat
colds while protecting against bacterial-related illness and infection.

KEYWORDS
Herbal tea, Plant, Usage areas, Health

Corresponding Author: TUBA ZBUCAK, tsiozbucak@hotmail.com

1
ORDU NVERSTES

1175
 Poster Session 8
Submission ID: 1112

AROMATIC HERBS THAT USED DURING PREGNANCY

1 1 1
VESLE KOAK , KAMLE ALTUNTU , EMEL EGE

ABSTRACT
Complementary and Alternative Medicine is a series of health approaches with a history of
use other than medical treatments. It covers a broad range of therapy divided into five categories:
alternative medical practices, mental and physical interventions, biological based treatments,
manipulative-body based methods and energy healing therapies (NIHS, 2017; Strouss, Mackley,
Guillen, Paul, & Locke, 2014). Complementary and Alternative Medicine (CAM) is a different
collection of health care. Women at maternal age represent one of the biggest group of CAM users.
The National Health Interview Study (NHIS) analysis in USA in 2007 reported that almost half of the
women at the age of 18-49 used a kind of CAM in the previous year (Holden, Gardiner, Birdee, Davis,
& Yeh, 2016). Many pregnant women throughout the world are increasingly using the alternative and
complementary treatment methods for their pregnancy problems as they regard them to be natural and
safe (Joa, Haeng Leeb, Moo Leea, Leec, Kwackd, & Kime, 2016). This ratio varies by 7% - 55%
depending on the geographical location and socio-cultural structure (Cuzzolin, Francini-Pesenti,
Verlato, Joppi, Baldelli, & Benoni, 2010). Pregnant women employ several methods to cope with the
problems of pregnancy. One of these methods is the use of herbal products. The most common
problems during pregnancy are nausea, heartburn, vomiting, skin problems, constipation and
indigestion (Al-Ramahi, Jaradat, & Adawi, 2013). Morover, there are other problems like toothache,
other pains of various type, flue, stomach-ache and chest pain (Mothupi, 2014). The mostly used
herbal products to cope with these problems are ginger, garlic, eucalyptus and tenaadam
(Rutachalenssis) (Al-Ramahi, Jaradat, & Adawi, 2013, Mothupi, 2014, Mekuria etal. 2017, Bayisa
etal. 2014, Laelago etal. 2016). Ginger is one of the best natural medicines known used for pregnancy
nausea and vomiting. Ginger appears to be useful for morning sickness but it is not clear whether it is
safe to use during pregnancy. Some studies indicate that ginger may potentially affect the foetal sex
hormones (Al-Ramahi, Jaradat, & Adawi, 2013). The pregnant women start the use of herbal products
on their own or by the recommendation of their families and they usually dont consult with health
professionals (Ekrasarian, Rostami, Charati, & Abdollahi, 2016, Kssal, evik Gner, & Batkn
Ertrk, 2017). Women like using these products despite the lack of sufficient evidence and safety
measures for the use of herbal products. During pregnancy, many women consider herbs to be natural
and risk-free, and they are not aware of their possible negative effects. (Kalder, Knoblauch, Hrgovic,
& Munstedt, 2011; Strouss, Mackley, Guillen, Paul, & Locke, 2014). The use of herbal treatment may
lead to abortion or premature birth, presence of malformation, intrauterine growth retardation and low
neonatal birth weight (Cuzzolin L., Francini-Pesenti, Verlato, Joppi, Baldelli, & Benoni, 2010). In
conclusion, pregnant women employ herbal products for their physiologic and psychological
problems, and they usually decide on their own what herbs to be used. Most of the pregnant women
believe that the herbal treatments are safe. The studies on the safety of herbal products are not
sufficient. There is need for evidence based studies on the use of herbal products during pregnancy.

Corresponding Author: VESLE KOAK, and.sile@hotmail.com

1
NECMETTN ERBAKAN NVERSTES SALIK BLMLER FAKLTES

1176
KEYWORDS
Pregnancy; Herbal Treatment; Alternative Treatment

1177
 Poster Session 8
Submission ID: 1113

EVALUATION OF YIELD AND QUALITY OF CORIANDER

(CORIANDRUM SATIVUM) IN TURKEY AND WORLD

1 1
GLSM YALDIZ , MAHMUT AMLICA

ABSTRACT
Coriander (Coriandrum sativum) is medicine and spice plant which is generally annual plant
from Apiaceae (Umbelliferae) family. It has special smell and it is rich in terms of micro and macro
nutrients. While seeds of coriander are rich as essential oil, it has vitamins with regard to leaves.
Linalool is the most component in coriander with 60-70%. Apart from linalool, cymene, camphor, y-
terpinen and a-pinene are also important components. In our country, it is common Mardin, Gaziantep
with big kernel variety of coriander and Erzurum, Van, sinop with small kernel. Coriander is grown in
Russia, Hungary, Poland, Bulgaria, England, Netherlands, Morocco and Egypt in the world. In
addition to, fresh herb of coriander is used Argentina, South America, Caucasus, Iran, Iraq and
Mexico. But the main producer countries are Ukraine, Russia, Morocco, Argentina, Mexico, Romania
and India. India corresponds to product of coriander about 25-40% in the world. The most raw oil was
obtained from Germany (28.4%) and the lowest essential oil was obtained from Bangledesh (10%)in
2012 year. In terms of essential oil, the lowest producer country was Brazil (0.03%) and the highest
producer was India (2.2%) in 2012 year. As raw oil and essential oil, Turkey had %16.5 raw oil and
0.9% essential oil in 2012. In 2013 year, Turkey exported 216 tons coriander seeds and had $543.000.
The highest average seed yield was produced in Portugal (1900 kg/ha), the lowest average seed yield
was obtained from pakistan (864 kg/ha). India and Netherlands had trade value in the world with $26
million and $3 million and amount of trade was similar trade value with 34 million(kg) and 4
million(kg), respectively. In this review, yield and quality of coriander was determined depended on
essential oil, raw oil, production of coriander, producer countries and Turkey, amount of production in
the world and Turkey.

KEYWORDS
Coriander, Yield, Export, Turkey, World

Corresponding Author: GLSM YALDIZ, g_yaldiz@hotmail.com

1
ABANT ZZET BAYSAL NVERSTES

1178
 Poster Session 8
Submission ID: 1114

A RESEARCH ON MEDICINAL PLANT CYCLAMEN COUM SUBSP.

COUM TAXA

2 1 1 2
TUBA BAYRAK ZBUCAK , GLAYCAN POLAT , ZNUR ERGEN AKN , HAMDI GRAY KUTBAY

ABSTRACT
Cyclamen coum subsp. coum Mill. (Primulaceae) is a perennial geophyte species which is
used as medicinal plant (boils treatment, stains and sunburn, gout, emetic, poisonous animal bites, in
lowering the cholesterol level in the blood and tinnitus). Local names of taxa which commonly used in
public are Yersomunu, Danagbei, Kzl meneke, Domuz ekmei, Domuz elmas, Domuz turpu,
Domuz ava and Tavankula. In this study, some of morphological characters were determined in
Cyclamen coum subsp. coum samples collected from different localities in Ordu province (Turkey)
depending on the elevational gradient (from sea level to 1850 meters). The shoot length, number of
nodes and branches, leaf length and width, living leaves, dead leaves, the number of flowers,
root/shoot ratio, specific leaf area (SLA), leaf weight/leaf area (LMA) were calculated separately in
the plant samples. According to statistically analyses there were found shoot length, bulb width, leaf
width, leaf length, numbers of branches and flowers were found statistically significant, whereas the
length of tubers, root-shoot ratio, number of living and dead leaves, number of node, the SLA and the
LMA values were not significant. Knowing these morphological properties is important for protecting
species and culturing.

KEYWORDS
Cyclamen coum var. coum, Geophyte, Morphology, Protecting

Corresponding Author: GLAYCAN POLAT, gulaycan.polat06@gmail.com

2
ORDU NVERSTES
2
ONDOKUZMAYIS NVERSTES

1179
 Poster Session 8
Submission ID: 1115

THE CHEMICAL COMPOSITION OF MILK THISTLE (SILYBUM

MARIANUS)

1 2 2
GLDEN DOAN , AZIZE DEMIRPOLAT , EYP BACI

ABSTRACT
Milk thistle purifies the liver from all toxic and harmful substances, supports the regeneration
of liver cells. In this study, the chemical composition of Milk thistle (Silybum marianum (L.) Gaertner
= Carduus marianus L.) from Turkey investigated. The chemical composition obtained by
hydrodistillation of was investigated by GC and GC-MS systems. The essential oils yield is 1.1%
(v/w). Eight constituents were comprised the 97.3% of the total oil extracted from the Milk thistle. The
major compounds of Milk thistle were determined as oleic acid (45.6%), linoleik acid (29.0%),
ethylbenzene (7.0%) and stearic acid (5.7%).

KEYWORDS
Milk thistle, Chemical Composition, GC-GC/MS, Turkey.

Corresponding Author: GLDEN DOAN, gdogan@firat.edu.tr

1
FIRAT NVERSTES FEN FAK. BYOLOJ BL./ELAZI
2
FIRAT NVERSTES

1180
 Poster Session 8
Submission ID: 1117

AN ECOLOGICAL STUDY ON MEDICINAL GEOPHYTE CYCLAMEN

COUM SUBSP. COUM

1 1 1 2
TUBA BAYRAK ZBUCAK , GLAYCAN POLAT , ZNUR ERGEN AKN , BURAK SRMEN

ABSTRACT
Turkey is a very rich country in terms of wild plant species. 816 geophytes taxa were
identified according to Tubives records. Geophytes are plants that survive part of their annual life
cycle as a dormant, fleshy underground structure (bulb, rhizome and tuber). Geophytes have been used
as food, medicinal, industrial and economic for many years. Geophytes were threatened by various
activities (overgrazing, industrialization, agricultural struggles, forest fires, new road opening, and
illegal collection) in our country. C. coum subsp. coum.Mill. (Primulaceae) is medicinal and
ornamental geophytes plant. Plant has emetic, laxative and stimulating effects. Its tuber is preferred
food by pigs. Local names are used in public are Alayaprak, Danagbei, Devetaban, Domuzekmei,
Domuzelmas, Domuzturpu, Krmenekesi, Kzlmeneke, Kstkpei, Kuskusa, Tavankula,
Topalak and Yersomunu. Plant is in CITES (Convention on International Trade in Endangered Species
of Wild Fauna & Flora) due to threatened by illegal collection. Therefore the ecological characters of
this species are important for its conservation. The aim of this study was investigated Reproductive
Effort (RE) values and soil properties of Cyclamen coum subsp. coum (Primulaceae) along an
elevation gradient. Reproductive effort (RE) is an important trait for the adaptive ability of a particular
species. RE1, RE2, RE3 and RE4 values were determined as RE value. RE values were calculated as
follows; RE1= flower biomass/ above ground plant part biomass, RE2=flower biomass/total plant
biomass, RE3=flower nitrogen concentration/above ground plant part nitrogen concentration, RE4=
flower nitrogen concentration/total nitrogen concentration. According to the results of study, the
lowest RE1, RE2 and RE3 values were found in 1850 meters while the lowest RE4 value was in 500
meters. The results of statistical analysis made for the reproductive effort values indicate that only
RE3 values were found to be significant. In soil samples taken from different altitudes was carried out
physical and chemical analysis. There were found sea level and 1850 m. localities have sandy-loam
soils while 500 m. locality has sandy-clay-loam soil. In addition, it has been determined locality of
1850 meters has strongly acidic while the sea level locality has weakly acidic in character.

KEYWORDS
Cyclamen coum subsp. coum, Medicinal plant, Conservation, Ordu

Corresponding Author: GLAYCAN POLAT, gulaycan.polat06@gmail.com

1
ORDU NVERSTES
2
ONDOKUZMAYIS NVERSTES

1181
 Poster Session 8
Submission ID: 1118

CHROMATOGRAPHIC AND SPECTROSCOPIC DETERMINATION

OF PHENOLIC ACID AND FLAVONOID CONTENTS WITH RADICAL
SCAVENGING ACTIVITY OF CUSCUTA CAMPESTRIS

1 1 2 1
EMINE AKYZ TURUMTAY , EMINE KILICKAYA SELVI , HALBAY TURUMTAY , ADEM DEMIR

ABSTRACT
As a holoparasitic plant, Cuscuta campestris Yuncker (C. campestris) is being valorized for
treatment of liver injury and cancer in traditional medicine. Phenolic acid and flavonoid content of C.
campestris were evaluated in this study by analyzing its diethyl ether, ethyl acetate, methanol, n-
butanol and water extracts. Antioxidant potential of these extracts were characterized with their total
phenolic (TPC) and flavonoid contents (TFC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical
scavenging activities. The individual phenolic compounds of all extracts were analyzed by HPLC-
DAD to illuminate the responsible antioxidants. Ethyl acetate extract revealed significantly highest
antioxidant effect, and also had highest TPC and TFC contents. The most amount of phenolic
compounds were quantified in methanol extract by HPLC-DAD analysis. Isorhamnetin, kaempferol
and quercetin were the major compounds of the methanol, ethyl acetate and diethyl ether extracts. The
most amount of rutin (quercetin-3-O-rutinoside) was found in the water extract. Ferulic acid was the
highest phenolic compound in the butanol extract. As a result, C. campestris, a rich source of phenolic
compounds, may be a reliable candidate for drug development.

KEYWORDS
Cuscuta, bioactive compounds, flavonol.

Corresponding Author: EMNE AKYZ TURUMTAY, emine.turumtay@gmail.com

1
RECEP TAYYP ERDOAN UNVERSTY
2
KARADENZ TECHNCAL UNVERSTY

1182
 Poster Session 8
Submission ID: 1119

DETERMINATION OF GERMINATION ABILITY OF BASIL IN

INCREASING SALT CONCENTRATIONS

1 1
MAHMUT AMLICA , GLSM YALDIZ

ABSTRACT
Salinity is one of the most environmental problems in arid and semi-arid region. It is an
abiotic stress factor which restricts crop production and affects development of plants. One of the
these plants is basil (Ocimum basilicum). It is an annual medicinal and aromatic plant from Lamiaceae
family. The present study was carried out to determine the response of different salt concentrations
from 0 to 240 mM which increasing 20 mM. The experiment was conducted with randomized
complete block design with 3 replications and placed 20 number from seeds of each plant in petri
dishes. 39 petris were used consisting from 1 plant x 13 salt levels x 3 replicats. Germination tests
were made at constant temperature (291 C), dark field and drying oven in laboratory conditions.
Appropriate test solution was placed at each petri dish being 5 ml and was renewed with an interval of
two days. According to the study results, germination speed and power of basil seeds completed
within 3-15 days. The results noted that root lenght changed between 0.08-5.07 cm, shoot lenght
changed between 0.1-5.82 cm in the basil and they changed between 10-100% germination rate
between the 0-240 mM salt concentrations. The lowest germination speed and power were seen in 240
mM and the highest germination speed and power were seen in 20 mM except control (pure water)
condition. Germination percentage of basil seeds were decreased from 0 (control) to 240 mM. In
addition to this, the highest shoot and root lenght were obtained from 20 mM and the lowest shoot and
root lenght were obtained from 240 mM except control condition. Root lenght/shoot lenght was also
determined changing between 0.43-1.27 cm. To results, it was determined that germination number
and rate, shoot and root lenght were statistically affected by different salt doses. Considering different
salt doses, the highest number of germination was obtained from 20 mM salt application, the lowest
value was determined in 240 mM salt application compared to control application. It is suggested that
salt tolerence studies should be preferred under 200 mM salt concentration to obtain the germination
number and rate over 80% in different salt concentrations and in order to grow the basil under salted
areas.

KEYWORDS
Basil, Salt, Germination, Root and shoot lenght

Corresponding Author: MAHMUT AMLICA, mcamlica25@outlook.com

1
ABANT ZZET BAYSAL NVERSTES

1183
 Poster Session 8
Submission ID: 1121

CHARACTERIZATION OF PHENOLIC COMPOUNDS AND THEIR

INHIBITORY EFFECT OF ROSA CANINA ON BACTERIAL DNA
POLYMERASES

1 2 2 2
HALBAY TURUMTAY , EMINE AKYUZ TURUMTAY , ADEM DEMIR , EMINE ESRA BUDAK , CEMAL SANDALLI

ABSTRACT
Investigation of novel plant-based agents might provide alternative antibiotics and thus fight
antibiotic resistance. Here, we measured the ability of fruit and leaf extracts of Rosa canina to inhibit
nonreplicative (Klenow Fragment-KF and Bacillus Large Fragment-BLF) and replicative (DnaE and
PolC) bacterial DNA polymerases along with their antimicrobial, DPPH free radical scavenging
activity (RSA), and chemical contents by total phenolic content and HPLC-DAD analysis. We found
that leaf extracts had nearly 10-fold higher RSA and 5-fold greater TPC than the corresponding fruit
extracts. All extracts had large amounts of chlorogenic acid (CGA) and rutin, while fruit extracts had
large amounts of quercetin. Hydrolysis of fruit extracts revealed mainly caffeic acid from CGA
(caffeoylquinic acid) and quercetin from rutin (quercetin-3-O-rutinoside), as well as CGA and
derivatives of CGA and p-coumaric acid. Plant extracts antimicrobial activity against Gram-negative
microorganisms. Thus, these species can be considered a potential source of novel antimicrobial
agents specific for Gram-negative bacteria.

KEYWORDS
Rosa canina, phenolics, DNA polymerase inhibition

Corresponding Author: HALBAY TURUMTAY, hturumtay@gmail.com

1
KARADENZ TECHNCAL UNVERSTY
2
RECEP TAYYP ERDOGAN UNVERSTY

1184
 Poster Session 8
Submission ID: 1780

A REVIEW ON SAFFRON

1 1
MERVE ZDEMIR , SERAY AKALIN

ABSTRACT

D
From the family of Irisaceae (Crocus sativus), an onion plant, saffron is known as the world's
most expensive spring. Moroccan, Indian, European, Arabic and Turkish cuisines are commonly used
for the purpose of coloring and adding flavor to foods and for treating traditional diseases in various

TE
diseases. Recent studies have begun to focus on the important effects of saffron on metabolism.
Research on the bioactivity of safronn has shown an antioxidant effect in living organisms. These
studies, carried out in a wide range of, confirm that the genotoxic chemicals of the saffron can inhibit
damage caused by it, suggesting that oxidative stress can be reduced in living organisms. It is a
EN
compound which gives the characteristic features of crocin, piccrocin and safranal plant. Along with
many other effects such as antioxidants, anticancer agents, immunomodulators and antidepressants,
saffron extracts, and potentially antitumor, antidepressant and memory healing properties of crocin,
are also promising for Alzheimer's, one of the major problems of our time. At the same time, a certain
amount of saffron consumption can be used to prevent the development of obesity thanks to digestive
ES
toxins are investigated. In recent years, saffron has been attracting attention due to the fact that it is a
natural resource in the field of new drug development with different effect mechanisms on diseases.
PR

KEYWORDS
saffron, disease, treatment
T
O
N

Corresponding Author: MERVE ZDEMR, merve_ozdemir@hacettepe.edu.tr

1
HACETTEPE NVERSTES

1185
 Poster Session 9
Submission ID: 57

ANATOLIAS SACRIFICIAL WILD FRUIT PYRUS ELAEAGRIFOLIA

(AHLAT) AND EVALUATION POTENTIALS

1 1 2 2 2 3
AYDIN UZUN , HASAN PINAR , YAAR AKIROLU , ARIF KARAKAYA , MEHMET UYSAL , GKHAN ZTRK ,
3 4 5 1
RECEP ALI EMRE , HAKAN KELE , MUSTAFA NL , MEHMET YAMAN

ABSTRACT
Pyrus elaeagrifolia Pall. is a species of wild fruit that is found naturally in our country
especially in the interior and passage areas. It has long been recognized that a wide variety of natural
varieties, both in-species and interspecies, is considered to be a species that grows with seeds that are
open pollinated in nature. It is foreseen that this rich diversity in the dialect, which is generally
observed to conform to calcareous and arid areas, has characteristics of resistance to biotic and abiotic
stress (drought, lime, salt, etc.) conditions especially for rootstock breeding studies. The existing P.
elaeagrifolia populations are utilized for different purposes in the regions of Anatolia in practice. Some
varieties of pears are grafted on P. elaeagrifolia, they can be used for afforestation purposes in rural
areas in arid conditions, fruits are consumed fresh, pickled or dried. It is known that P. elaeagrifolia is
a useful product for diarrhea cutter, heart and kidneys and is used among the people. The small, and
very sandy structure, is regarded as an animal feed. In addition, the branches are utilized in cane
construction. With these respects, ahlat can be used for the benefit of human beings and animals for
medical and nutrition purposes as well as being developed and cultured. It is very important to protect
and develop this kind of adaptation to the Anatolian steppes and guarding it.

KEYWORDS
Pyrus elaeagrifolia, wild fruits, ethnobotanic

Corresponding Author: AYDIN UZUN, uzun38s@yahoo.com

1
ERCYES UNVERSTES BAHE BTKLER BLM
2
ORMAN VE SU LER BAKANLII LLEME VE EROZYONLA MCADELE GENEL MDRL
3
MEYVECLK ARATIRMA ENSTTS-ERDR
4
MERSN ORMAN BLGE MDRL
5
ALATA BAHE KLTRLER ARATIRMA ENSTTS

1186
 Poster Session 9
Submission ID: 59

DETERMINATION OF IMPORTANT FRUIT CHARACTERISTICS OF

PYRUS ELAEAGRIFOLIA, A WILD FRUIT SPECIES SELECTED
FROM KAYSERI AND USE FOR HUMAN NUTRIENT

1 1 2 2 2 1
AYDIN UZUN , HASAN PINAR , YAAR AKIROGLU , ARIF KARAKAYA , MEHMET UYSAL , MEHMET YAMAN ,
3 2
KAHRAMAN GURCAN , MUAMMER COKUN

ABSTRACT
The motherland of Anatolia, wild pear (Pyrus elaeagrifolia Pall.) is one of 22 Pyrus species
and spread in Southeast Europe, Russia and Turkey. Natural spreading areas in our country are
reported as Ktahya, Eskiehir, Bolu, stanbul, Kastamonu, Sivas, Ankara, Antalya and Kayseri. The
trees are medium-sized, rounded crowned, often branched and thorny. The fruit is small, round and
very smooth. Pyrus elaeagrifolia is deeply rooted, fully xerophilic, well adapted to the arid climatic
conditions. There are of this species is mainly used as fresh fruit in the countryside, dried and pickled
and can be used for nutrition purposes, as it is beneficial to the heart and kidneys with diarrhea
treatment firstly in the folk medicine. In addition, cultured pears are vaccinated on matrices and
evaluated for rootstock purposes. On the other hand, sandy and small fruit that is not consumed by
human beings is regarded as animal feed. The main factors for the consumption of Pyrus elaeagrifolia
fruits are fruit size and sandy. There are important variations in the natural genotypes in this respect. In
this study, which is one of the important spreading areas of this species, Kayseri region, fruit
characteristics were revealed in different types of populations and it was aimed to determine the types
that are more suitable for human consumption. The study presents beneficial results for the evaluation,
development and conservation of this naturally grown population where people can evaluate for
different purposes.

KEYWORDS
Pyrus elaeagrifolia, fruit characteristics, genetic resources

Corresponding Author: AYDIN UZUN, uzun38s@yahoo.com

1
ERCYES NVERSTES BAHE BTKLER BLM
2
ORMAN VE SU LER BAKANLII LLEME VE EROZYONLA MCADELE GENEL MDRL
3
ERCYES NVERSTES TARIMSAL BYOTEKNOLOJ BLM

1187
 Poster Session 9
Submission ID: 1122

AN IMPORTANT FLAVONOID FOR NUTRITION: QUERCETIN

1 1
MUHAMMET ALI CEBRBAY , NAZAN AKTA

ABSTRACT
The increase in diseases such as cancer that have occurred based on oxidative degeneration in
nutrition-related cells in the recent years has generated interest in the pharmacokinetic properties of
various bioactive molecules, particularly the antioxidants in foods to reduce the effects of oxidative
stress. Quercetin is important for nutrition because it is a well-known flavonoid which is very
commonly found in plants and it has potential functional properties. This study aimed to compile the
findings about the relationship of quercetin with nutrition, sources of quercetin, its biological activity
and metabolization. As a result, it emphasized that quercetin has various positive effects (e.g., anti-
inflammatory, antihistaminic) on cells, particularly on oxidative stress. Quercetin is found in many
vegetables and fruits (e.g., citrus fruits, onions, tomatoes and capers) or foods obtained from them
(e.g., wine) based on carbohydrates, ethers or phenolic acids as secondary metabolites. It is associated
with many nutrition-related diseases, particularly with some cardiovascular diseases. Quercetin plays a
metabolic role in glucuronidation, sulfation and methylation reactions in the human body. Further
detailed studies which will determine the relationship of quercetin with nutrition should be conducted,
and awareness about its effects on health should be raised.

KEYWORDS
Quercetin, nutrition, flavanoids

Corresponding Author: MUHAMMET AL CEBRBAY, cebirbay@gmail.com

1
SELUK NVERSTES SALIK BLMLER FAKLTES BESLENME VE DYETETK BLM

1188
 Poster Session 9
Submission ID: 1123

THE FUNCTIONAL FOOD: WALNUT

1 2 1
BAAK ZDEMR , SAADET SEVL YCEL , FATMA YEM OKAY

ABSTRACT
Walnut is a valuable nutrient in terms of human nutrition and health and also it is a source of
energy because of its fat content (59 - 74%). It provides about 700 calories per 100 g. Due to walnuts
several benefits to human health, it is remarkable precious nutrition which is wealthy with regards to
fatty acids and tocopherols. Walnut oil is rich as unsaturated fatty acids and for this reason walnut has
a great value in terms of health. Besides, walnut contains protein (14 - 24%), mineral compounds (1.5
- 2%), cellulose (5 - 10.5%), it is rich with B and D vitamins and also it contains A, C and E vitamins.
Shelled walnuts are containing high amounts of potassium, phosphor, magnesium, calcium, sulphur
and iron. Unsaturated fatty acids in walnut have an effect of decreasing the risk of cardiovascular
diseases. Linoleic, oleic, linolenic, palmitic and stearic acids enable to increase HDL cholesterol and
reduce LDL cholesterol, thereby walnut has an effect on cardiovascular diseases such as protector.
Mineral compounds as potassium and magnesium regulate blood pressure. When consumed
frequently, it provides protection from coronary heart disease. Juglone which is obtained from walnut
roots has an effect of reducing gall deflection. Due to its antioxidant and phytochemical compositions,
walnut avoids cell destruction of the free radicals. Besides, it enhances body resistance thereby walnut
plays as a prohibitor against diabetes. It reduces cancer risk by means of its polyphenol content. High
amount of omega-3 fatty acids make walnut a brain food and also it contains ready form of
melatonine. Also walnut is accepted as functional food because it supplies special physiological effect
in body requirement and displays activity in cure besides fulfills a need of basic nutrition. Walnut oil
is used in pharmaceutical industry, its leaf, root and green shell are processed in various forms for
using in some cures of diseases, also dye and tanin are obtained from walnut. After obtaining walnut
oil, the rest of residue has a nutrient value for animal nutrition in terms of fat and protein content. It
should not be forgotten that, in addition to walnut kernel, walnut kernel pellicle also has high
nutritional and health values. There is increasing evidence that consumption of whole foods is better
than isolated food components such as dietary supplements and nutraceuticals. It is emphasized that
whole food such as walnut is more effective than dietary supplements in increasing LDL oxidation
resistance, lowering DNA damage, and inducing higher repair activity in human volunteers.

KEYWORDS
Walnut, Nutrition content, Health, Functional food

Corresponding Author: BAAK ZDEMR, basakcnr87@hotmail.com

1
ANKARA NVERSTES ZRAAT FAKLTES
2
BLENT ECEVT NVERSTES GKEBEY MTHAT-MEHMET ANAKCI MESLEK YKSEKOKULU

1189
 Poster Session 9
Submission ID: 1124

ST JOHNS WORT (HYPERICUM PERFORATUM L.): ITS

COMPOSITION AND CLINICAL PROPERTIES

1 1 1
BIRSEN YILMAZ , GAMZE AKBULUT , NILFER ACAR-TEK

ABSTRACT
There are about 400 species of St John's wort in the world belonging to the Hypericum genus
Clusiaceae family and the Hypericoideae subfamily. It is reported that the herb has about 80 types.
The most common types in our country are Hypericum perforatum L., H. Trigqetrifolium, Hypericum
calycinum, H. empetrifolium Willd. (heather, yellow heather), H. scabrum L. (mexican herb, kepir
otu) and H. tedrapetum Fries. It is best grown in light acidic-neutral soils, mainly in Europe, Asia,
North Africa and the United States. St John's wort is known in our country with different names such
as tipton's weed and felty germander. It is a perennial plant with golden yellow flowers, ranging in
height from 30 to 90 cm. The chemistry of St John's wort is quite complex and the available data on
the active ingredients is not clear. It is also reported that the plant has different composition depending
on its dry/wet status, and some components have intensified/decreased in certain areas. The major
groups of dry extracts are phenyl propanoids, flavon glycosides, biflavones, oligomeric
proanthocyanins as well as floroglucinols, xanthones and naftodiantrons. The phenylpropanoids in St
John's wort include p-coumaric acid and caffeic acid. The anthracene derivatives especially in the
hiperisin and pseudohyperaceous are found in the foliage and perforated parts of the flowers and give
a red color to the oil of St John's wort. In general, when the components of the plant are evaluated, a
number of compounds with biological activity are mentioned, especially anthracene derivatives
(hypersecin and pseudohypercin), cholinergic acid, flavonoids, phenolic compounds (hyperforin),
procyanidins, vitamin C, carotene, protein, resin and essential oils. St John's wort has been known with
the healing effect of injuries for many years. Recently, the antidepressant effect has been proven and
the use has become widespread. Accordingly, it is used particularly in the treatment of mild and
moderate depression, cancer, diabetes, chronic rheumatism, some gastrointestinal diseases as well as
throat infections, colds, antiseptic and burn wounds. In the treatment of mild and moderate depression,
the daily dose is 500 mg extract (500 mg extract contains 1-2 mg of hypericin). It has been reported
that hypericin, one of the major causative agents, may be effective in the treatment of AIDS as well as
depression and cancer. However, as with many plant extracts, St John's wort also require extensive and
long-term investigations to better understand the mechanisms of action and possible
beneficial/deleterious effects.

KEYWORDS
St Johns wort, health, aromatic plant

Corresponding Author: BRSEN YILMAZ, dytbirsen@gmail.com

1
GAZ NVERSTES

1190
 Poster Session 9
Submission ID: 1125

SYNTHESIS AND CHARACTERIZATION OF SILVER

NANOPARTICLES USING GREEN TEA EXTRACT

1 1 2
GNL SERDAR , CANSU ALBAY , MNEVVER SKMEN

ABSTRACT
Synthesis and Characterization of Slver Nanopartcles Using Green Tea Gnl SERDAR1,
Cansu ALBAY1, Mnevver SKMEN2 1Karadeniz Technical University, Faculty of Pharmacy
Department of Analytical Chemistry, 61080 Trabzon, 1Karadeniz Technical University, Faculty of
Pharmacy, Department of Biochemistry, 61080 Trabzon, 2Konya Food and Agriculturel University,
Faculty of Engineering and Architecture, Department of bioengineering, 42080 Konya
gonulserdar@ktu.edu.tr In this study, the synthesis and characterization of Ag nanoparticles from
green tea collected the Eastern Black Sea region was investigated. Microwave extraction was applied
to caffeine and catechin coextraction from tea samples. A household microwave system device was
used for microwave extraction. 10 g of sample was shaken in 100 mL of water for 90 min at room
temperature and then extracted in a household microwave device at 90 C, 3 minutes, 360 W. Ag
nanoparticles were synthesized that the obtained aqueous solution was taken in different volumes by
mixing 100 mL of 1mM AgNO3 solution at room temperature of 0-60 min for different times. Uv-
Visible spectroscopy is one of the widely used techniques for the characterization of nanoparticles. A
yellow coloration appeared, indicating the onset of Ag nanoparticle formation. The progress of the
reaction was monitored by measuring the absorbance of the solution at regular intervals of time.
Absorption spectra were measured on a Shimadzu UVP-1240 spectrophotometer. Keywords: Green
tea, Ag tanoparticul, Microwave Assisted Extraction, UV-Visible REFERENCES: [1]. Loo, Y.Y.,
Chieng, B.W., Nishibuchi, M., Radu, S., 2012. Int. J.Nanomed.,7, 42634267. [2]. Jia, J.L., Xu, H.H.,
Li, D.Q., Ye, W.H., Liu, W.J. 2015. Synthesis and Reactivity in Inorganic, Metal-Organic, and Nano-
Metal Chemistry, 45, 941946. [3]. Khalil, M.M.H., Ismail E.H., El-Baghdady, K.Z., Mohamed, D.
2014. Arabian J. Chem., 7, 11311139.

KEYWORDS
Green Tea, Ag tanoparticul, Microwave Assisted Extraction, UV-Visible

Corresponding Author: GNL SERDAR, gonulserdar@ktu.edu.tr

1
KARADENZ TEKNK NVERSTES
2
KONYA GIDA VE TARIM NVERSTES

1191
 Poster Session 9
Submission ID: 1127

ISOLATON OF SECONDARY METABOLITES FROM AN ENDEMC

PLANT SPECES TANACETUM ALYSSIFOLIUM AND
DETERMNATON OF ANTOXDANT-ANTPROLFERATVE
PROPERTES

1 1 1
YAKUP ULUTA , EKREM KKSAL , AHMET ALTAY

ABSTRACT
Secondary metabolites, synthesized by plants, both during normal development and in
response to stress conditions are phenolic compounds. The intake of these compounds is an important
health-protecting factor. Reactive oxygen species responsible of oxidative stress are involved to the
chronic diseases such as atherosclerosis, cancer, obesity, diabetes, and coronary diseases. Many
studies with different type of species of tanacetum medicinal plant revealed many bioactive properties
of these plant species such as antioxidant, antimutagenic and anti-carcinogen. The aim of this study
was to isolate the available secondary metabolites in Tanacetum alyssifolium plants by column
chromatography and determine their structures with spectroscopic methods as well as their antioxidant
and antiproliferative activities. For this purpose, Tanacetum alyssifolium plants was collected from the
foothills of Munzur Mountains in Erzincan province and dried at room temperature. The upper part of
ground plant was extracted with ethyl acetate/ butanol solvent system. The butanol extract was
subjected to the procedure of column chromatography. The structures of the isolated compounds were
elucidated by spectroscopic methods (1 H-NMR, 2 D-NMR, HPLC-TOF) to reveal that luteolin 7-o-
glycoside, umckaline and S-8-H29. The antioxidant activities of the isolated compounds were
evaluated according to the DPPH and ABTS free radical scavenging and metal ion chelating
capacities. Antiproliferative activities of the samples was investigated on HeLa (Human Rectum
Cancer) and C6 (Rat Brain Tumor) cell lines and compared with 5-Fluorouracil (5-FU), which is used
as an anticancer drug. In the antiproliferative activity tests, n-butanol extract, Umckalin, Luteolin 7-o-
glycoside and S-8-H29) showed lower activity than 5-FU against both HeLa and C6 cell lines at four
different concentrations (5, 25, 50 100 g/ml). The antiproliferative activities of the samples against
the HeLa cells at the highest dose of 100 g/mL were; 5-FU> butanol extract> Umckaline> S-8-H29>
Luteolin 7-o-glycoside, respectively. On the other hand, antiproliferative activities against C6 cells
were observed as 5-FU> butanol extract> Luteolin 7-o-glycoside> Umckaline> S-8-H29, respectively.

KEYWORDS
Tanacetum alyssifolium, isolation anticancerogen activity, antioxidant activity, secondary
metabolite

Corresponding Author: YAKUP ULUTA, ykp.ulutas@gmail.com

1
ERZNCAN NVERSTES

1192
 Poster Session 9
Submission ID: 1128

EVALUATION OF INFORMATION AND REFLECTIVES OF

COMPLEMENTARY AND ALTERNATIVE METHODS OF HEALTH
SERVICE VOCATIONAL SCHOOL STUDENTS

1 1 1 1 1
DIDEM SARIMEHMET , NURHAN GMRKOLU , SEVILAY HNTSTAN , BAHAR CANDA , GONCA SERDAR

ABSTRACT
Objectives:The study was conducted as a descriptive study to determine the knowledge and
thought of the students attending Karadeniz Technical UniversityHealth Services Vocational School
(KTU SHMYO) on Alternative and Complementary Medicine Methods: The study group consisted of
230 individuals studying at KTU SHMYO. Data was collected from December 2016 to March 2017
using a questionnaire. Numbers of percentage test was used in evaluating the data. Findings: 70.8% of
the students were found not to use the CAM methods at all. It was determined that the students who
used CAM especially preferred to use these methods in order to decrease their stress (%30.9), for their
health problems (%26) and hair and facial treatment (%11.6). Most of these methods were seen / heard
via internet (39.9%) and friends (16.7%). The use of herbal products (59.2%) was found to be the
generally recognised; while massive massaging (13.3%) was the most commonly known CAM
method. Results: Students generally do not have detailed knowledge of CAM methods. It was found
that the methods used more frequently in everyday life were even more recognised by the students

KEYWORDS
Complementary Treatment, Alternative Treatment, Student

Corresponding Author: DDEM SARIMEHMET, didemsarimehmet@ktu.edu.tr

1
KARADENZ TEKNK NVERSTES

1193
 Poster Session 9
Submission ID: 1129

ASSESSMENT OF IN VITRO ANTIGENOTOXIC EFFECT OF -

TOCOPHEROL AGAINST MITOMYCIN-C INDUCED
MICRONUCLEUS FORMATION

1 2 2 2
KBRA SEVIMLI CAN , FATMA UNAL , ECE AVULOLU YILMAZ , DENIZ YZBAIOLU

ABSTRACT
Alpha-tocopherol (AT), better known as Vitamin E (Vit-E), is an important compound in cell
membranes and has specific biological effects on regulating gene expression, signaling, cell
proliferation and reproduction. Alpha-tocopherol is present in a lot of different vegetables such as
spinach, turnip greens, broccoli, asparagus, tomatoes, mango, and orange vegetables such as carrots.
Many edible nuts such as almonds, hazelnut, pine nuts, peanuts, and pistachios are good sources of
AT. Vegetable oils such as wheat germ oil, sunflower, safflower, grape seed, almond, corn, peanut and
olive oils are another important sources of AT. Vit-E is a primer biological antioxidant and enables
prevention of oxidation reactions. It also plays a role in the reduction of free radicals, in this way it
provides protective property against potential toxic effect of highly reactive compounds. In this study,
possible antigenotoxic effect of -tocopherol, known as vitamin E, against Mitomycin C (MMC)
(antitumor agent) induced genotoxic damage was investigated. For this purpose, micronucleus (MN)
assay was performed in human peripheral lymphocytes in culture from two donors, a woman and a
man. Different concentrations of -tocopherol (25, 50, 100 and 200 g/mL) were used in combination
with 0.20 g/mL MMC. In the treatment of lymphocytes, the following groups were studied. 1)
pretreatment with -tocopherol 1 h before MMC; 2) simultaneous treatment with -tocopherol and
MMC; 3) post-treatment with -tocopherol 1 h after MMC. Lymphocytes were treated with MMC
alone or together with -tocopherol for 24 h and 48 h. In all the treatments, -tocopherol reduced the
frequency of MN compared to MMC alone. In the 24 h pre-treatment group, the frequency of MN
decreased significantly at the highest concentration of -tocopherol compared to positive control. In
the simultaneous treatment, a statistically significant reduction was observed at 50 and 200 g/mL
concentrations of -tocopherol compared to positive control. In the post-treated lymphocytes, none of
the reductions in MN frequency was significant. In 48 h pre-treatment group, a significant reduction of
MN frequency was observed at 100 g/mL concentration of -tocopherol compared to the positive
control. In simultaneous and post-treatment (except 100 g/mL) groups, MN frequency decreased non
significantly at all the concentrations. The result of this study showed that MMC increased the
frequency of MN in lymphocytes. The antigenotoxic experiments indicated that -tocopherol was able
to ameliorate the genotoxic effect induced by MMC.

KEYWORDS
-tocopherol, mitomycin C, antigenotoxicity, micronucleus test

Corresponding Author: FATMA UNAL, funal@gazi.edu.tr

1
2
GAZ NVERSTES

1194
 Poster Session 9
Submission ID: 1130

SAGE AND HYPOGLYCEMIC EFFECT

1 1
SERKAN ASLAN , EDA KKSAL

ABSTRACT
Sage (Salvia officinalis) is commonly located in Turkey produced medical aromatic plants.
The genus Salvia is distributed all over the world, with belonging to the Lamiaceae family represented
by over 900 species. Salvia officinalis, one of the Salvia species, is one of the important species due to
its medicinal and aromatic character. The botanical name of sage is a clear reference to the plant of the
genus name Salvia comes from the Latin salvre meaning "to save" or "to heal" and Officinalis means
medicinal. Studies on animals have shown that more than 400 plant species have a hypoglycemic
effect. One of these plants is Salvia officinalis. Salvia officinalis has been used as a traditional
treatment of diabetes in many countries, and glucose-lowering effects have been shown in animal
studies. Diabetic patients have increased oxidative stress and impaired antioxidant defense systems,
which appears to contribute to the onset and progression of diabetes-related complications. It is
believed that the antidiabetic effect of the sage is caused by its high antioxidant content. Its flavonoids
and polyphenolic compounds (Carnosic acid, rosmarinic acid, caffeic acid) have a strong antioxidant
and radical cleansing role. A methanol extract of Salvia officinalis given intraperitoneally in diabetic
rats significantly reduced blood glucose without any change in insulin levels in rats. In another study,
Aqueous ethanol extracts of Salvia officinalis have been found to significantly reduce blood glucose in
healthy rats and to significantly reduce hyperglycemia in mildly type 1 diabetic rats. Tea-infusions of
Salvia officinalis have been shown to possess similar effects in vitro. Salvia officinalis administered
by oral route showed significant hypoglycemic activity in diabetic rats. In a study conducted with
humans, no effect was observed on fasting blood glucose of sage tea (300 ml twice daily). However,
drinking sage has improved lipid profile and antioxidant capacity and has been shown to benefit
indirectly from diabetes. In conclusion, many in vitro studies show that the antidiabetic effect of sage
tea is insufficient to study on humans. More research is needed in this area to determine factors such
as quantity, infusing type and duration that are effective on humans. It is important for diabetics to be
warned about sage tea use and hypoglycemic effects.

KEYWORDS
date palm, antioxidant, nutrition

Corresponding Author: SERKAN ASLAN, serkanaslan43@gmail.com

1
GAZ NVERSTES/SALIK BLMLER FAKLTES/BESLENME VE DYETETK BLM

1195
 Poster Session 9
Submission ID: 1131

MEDICINAL USES OF EVERNIA PRUNASTRI

1 1 1 1
CELALEDDIN ZTRK , HATICE ESRA AKGL , GIYASETTIN KAIK , SINAN ALKAN

ABSTRACT
Evernia prunastri (L.) Ach. also known as oakmoss is a species of lichen that represents
symbiotic organisms consisting of the mycobiont and photobiont. The use of lichens in medicine is
based on the fact that they contain unique and varied biologically active metabolites. Oakmoss is used
in medicine due to its antiseptic, demulcent, expectorant and restorative properties. Its essential oil is
also used in certain perfume compositions. Here; we review the medicinal efficacy of Evernia
prunastri which intends to explore the pharmaceutical potential of the lichen substances.

KEYWORDS
Active metabolites, Evernia prunastri, Oakmoss

Corresponding Author: HATCE ESRA AKGL, h.esakgul@gmail.com

1
SELUK NVERSTES

1196
 Poster Session 9
Submission ID: 1132

EFFECT OF DRYING TEMPERATURE AND AIR VELOCITY ON

BIOACTIVE COMPOUNDS OF DRIED CORNELIAN CHERRY PUREE

1 2 2
SMAIL TONTUL , EMRAH EROGLU , AYHAN TOPUZ

ABSTRACT
Cornelian cherry (Cornus mas L.) is a rich source of bioactive compounds such as
anthocyanins, ascorbic acid and phenolics. Therefore, it has different health beneficial effects such as
antioxidant activity, anti-inflammatory effect, cardioprotective, and lipid-lowering properties.
According to Turkish Statistical Institute, the production of cornelian cherry in Turkey was higher
than 10000 tons in 2015. The produced cornelian cherry used in production of jams, marmelades and
nectar in Turkey. However, it is also used to produce different alcoholic and non-alcoholic beverages
due to its astringent properties. Moreover, it is also used in cosmetic and ornamental industries. There
are some studies on drying of whole cornelian cherry fruits using different drying techniques.
However, usability of the dried whole fruit is limited. Therefore, in the current study, cornelian cherry
puree was dried using hot air drying technique. By this way, a dried product which can be used as
additive or colorant in different food formulation was produced. In the current study, the effect of
drying temperature (50, 60 and 70C) and air velocity (1, 2 and 3 m/s) on total monomeric anthocyanin
content, radical scavenging activity and ascorbic acid content of the dried cornelian cherry puree was
determined. The study was conducted according to factorial design and all drying experiments were
carried out until moisture content of the final product reached 7.61-8.57 g/100g which corresponded
0.345-0.356 water activity. Total monomeric anthocyanin content of dried cornelian cherry puree was
determined in the range of 213.2-304.4 mg C3G/100g dm. According to the statistical analyses, drying
temperature, air velocity and their combination had significant effect on total monomeric anthocyanin
content of the samples. The highest total monomeric anthocyanin content was determined at 3 m/s air
velocity regardless of drying temperature. Radical scavenging activity of the samples were ranged
between 751.3-885.6 mg trolox equivalent/100 mg dm. The highest radical scavenging activity was
determined in the samples dried at 70C at 2 m/s air velocity while it was lowest at 60C at 1 m/s.
Ascorbic acid content of the dried cornelian cherry puree was determined between 677.5 and 1462.8
mg/100g dm. Both factors and their interaction were found to be significantly effective on ascorbic
acid content of the samples. Generally, increasing drying temperature had negative effect on ascorbic
acid content. The air velocity showed huge differences according to drying temperature. Overall,
drying at 60C drying temperature and 3 m/s air velocity provided dried cornelian cherry puree with
highest content of total monomeric anthocyanin, radical scavenging activity and ascorbic acid content.
The product produced using these conditions had total monomeric anthocyanin, radical scavenging
activity and ascorbic acid content of 297.3 mg C3G/100 g dm, 854.6 mg trolox equivalent/100 mg dm

Corresponding Author: SMAL TONTUL, itontul@konya.edu.tr

1
NECMETTN ERBAKAN UNVERSTY, FACULTY OF ENGNEERNG AND ARCHTECTURE, DEPARTMENT OF
FOOD ENGNEERNG, KONYA, TURKEY
2
AKDENZ UNVERSTY, FACULTY OF ENGNEERNG, DEPARTMENT OF FOOD ENGNEERNG, ANTALYA,
TURKEY

1197
and 1222.9 mg/100g dm, respectively. The study clearly showed that, dried cornelian cherry puree can
be used as an additive to increase functional properties of different food products.

KEYWORDS
cornelian cherry; drying; total monomeric anyhocyanins; radical scavenging activity;
ascorbic acid

1198
 Poster Session 9
Submission ID: 1133

ROSEMARY (ROSMARINUS OFFICINALIS L.): ITS COMPOSITION

AND CLINICAL PROPERTIES

1 1 1
BIRSEN YILMAZ , GAMZE AKBULUT , NILFER ACAR-TEK

ABSTRACT
Archaeological finds from the early ages indicate that people have benefited from many
features of plants, especially in terms of obtaining nutrients and improve the health problems. Over the
years, the interaction between man and plants has increased and now the ethnobotany field is born.
The term "phytotherapy", which means treatment with medicinal plants, was first used by Henri
Leclerc. The rosemary (Rosmarinus Officinalis L.) is also found in medicinal aromatic plants which
are widely produced in Turkey. Rosemary is a valuable essential oil and spice plant from the
Lamiaceae family and is in the form of a half-brier or brier. R. officinalis is the most important
rosemary species cultured. Rosemary, a plant that remains green all seasons, is a strong antioxidant.
Rosemary come out with leaf (Rosmarini folium) and volatile oil (Rosmarini aetheroleum) In
European Pharmacopoeia has many effects on health as well as the commercial use of it. Rosemary
essential oil is especially important in perfume, cosmetics and aroma therapy. A lot of research has
been done about secondary metabolites of rosemary. As a result of these investigations, it has been
reported that rosemary has anticancer, insecticide, antimicrobial and antioxidant effects. It is known
that rosemary is used by people in various fields such as headache, diuretic, rheumatic diseases and
wounds healing. Studies have concentrated on herbal antioxidants, as synthetically obtained
antioxidants can have toxic effects. Antioxidant molecules in rosemary are defined as carnosic acid,
carnosol, rosmaridifenol and rosmarinic acid in ethanol soluble fractions. Moreover, antioxidant
properties are found in the essential oil fractions of the rosemary. In addition to the positive properties
of antioxidants, it has been reported that they can be prooxidants and this can stimulate free radical
reactions. It has been shown that the composition of the rosemary is influenced by environment,
agronomic conditions, harvest time, storage period, age of the plant, developmental stage of the plant
and harvested organ. For this reason, there is a need to study more about health effects.

KEYWORDS
rosemary, medicinal plant, health

Corresponding Author: BRSEN YILMAZ, dytbirsen@gmail.com

1
GAZ NVERSTES

1199
 Poster Session 9
Submission ID: 1134

HELICHRYSUM SP.: KNOWN AND EVALUATED BY THE COMMON

FOLK EASTERN ANATOLIA AGRICULTURAL RESEARCH
INSTITUTE MANAGEMENT SOIL AND WATER RESOURCES
CAMPUS, TURKEY

1 1
DR.SIBEL KADIOLU , DR BANU KADIOLU

ABSTRACT
Abstract In an ethno-botanic study carried out between 2013 and 2016 in Kop Pass, 14
villages were visited to record information about the plants used by the people. In 9 visited villages
(Akduran, Kopky, Taal, rence, Demirka, Src, Altnta and Bamagil, Kapkale) it is
recorded that people are utilizing one of the Helichrysum species which they call yellow flower as a
medicinal herb. The Helichrysum species that are used to decorate the ceilings and the vases at the
homes are known with the names such as yellow flower, grapefruit and amaranthus. It is commonly
used as herbal tea. It is noted that the herb is used internally or externally for illnesses such as stomach
ache, infection, ulcer, wound, cancer, kidney stone, urinary track infection and arthritis. Helichrysum
sp. is a common plant in our country even though its originally from Europe. It has 34 naturally
growing species half of which are endemic. Because of this reason it is known with different names in
each region (Tr. Altn iek, Altn otu, Sariek, Mantuvar otu, Yayla iei, Herdemtaze, Solmaz
iek, Gne iei, Gve otu, Uluda iei, Kudama, Ar iei, Dudiye iei, Yahudi otu, Sar
kediaya, etc.). Medicinal plants has long-lasting usage for treatment purposes. Even though they lost
their importance to some extent due to development of synthetic medicines in this area of industry,
raw materials of many medicines are obtained from plants. Therefore, ethno-botanic studies that
gathers the folkloric information should be increased and the result of these studies should be
evaluated in pharmacologic and pharmacognosy researches.

KEYWORDS
Key Words: Everlasting Flower, Helichrysum, Pharmacognosis, Herbal tea, Ethno-botanic

Corresponding Author: DR.SBEL KADIOLU, sibel.kadioglu@hotmail.com

1
DOU ANADOLU TARIMSAL ARATIRMA ENSTTS MDRL

1200
 Poster Session 9
Submission ID: 1136

ETHICAL FRAMEWORK FOR THE USE OF VEGETABLE

PRODUCTS

1 1 1 1
DIDEM SARIMEHMET , NURHAN GMRKOLU , SEVILAY HNTSTAN , GONCA SERDAR

ABSTRACT
The herbal products have been used for thousands of years in many different cultural histories.
For reasons such as the increase of the expectancy of life, the fail of success in preventing and treating
chronic diseases, economic inadequacy, pressure from media, ineffectiveness of modern medicine and
fear or dissatisfaction from medical care; both health care providers and patients pursuit different
approaches. The most familiar of these approaches is the use of herbal products. Supportive attitudes
and proposals of individuals or institutions, whose identities are not sufficiently known in terms of
"competence", that are not based on the scientific basis, the use of herbal products like "medicine" and
collective use through written and visual media cause health workers to worry about this issue. Due to
these discomforts, the development of an approach that draws the scientific and ethical aspects of the
subject has has become a matter of importance by the institutions and individuals working in this area.
The aim of this compilation is to provide information on the importance of the use of herbal products,
ethical aspects and the responsibilities of nurses in this subject

KEYWORDS
Herbal Products, Ethics, Medicine, Nursing

Corresponding Author: DDEM SARIMEHMET, didemsarimehmet@hotmail.com

1
KARADENZ TEKNK NVERSTES

1201
 Poster Session 9
Submission ID: 1138

RHEUM RIBES L.: MIRACULOUS PLANT OF TURKEY ( IN

ERZURUM)

1 1
DR. BANU KADIOLU , DR. SIBEL KADIOLU

ABSTRACT
Abstract Rheum ribes is from Polygonaceae family that is one of the primary medicinal plants
with distinctive aromatic taste and rich content. The plant that grows in may and june is found more in
eastern regions of our country. It can grown in heights (1800-2800 or 2300-2700 or 1000-4000
meters), on rock and pebbled slopes. Rheum ribes is generally consumed fresh. Peeled stems of the
flowers growing among wide and rough leaves of the plant that appears after snow melts are
consumed. The versions of Rheum ribes known as gn or egn in Erzurum, in local language are
kn, gn, kn, ukun, ugun, ugun, ukun, agn, akn, ekin, egi. in Divanu Lgatit-Trk,
Rheum ribes is defined as a plant with a red flower whoose sherbet is good for polio. The edible part
of Rheum ribes of Anatolia is stem of flower whereas edible part of this plant growing in England
Rheum rhaponticum is stem of leaf. Today, in Erzurum Rheum ribes is consumed not only out of
joy but also for medicinal purposes. It has common folkloric usage to treat diabetes and hemorrhoid.
Rheum ribes that has rich Vitamin-C reserve contains Vitamins A, B1, B2, E and K. In scientific
studies it is observed that Rheum ribes plays an important role for cell renewal and development. It
can easily pollinate and interbreed with other species of its genus. Due to this feature, which is
important for research studies, it has common usage in pharmacologic researches. However, in
Anatolia there is a risk of extinction of Rheum ribes collected unconsciously and by inappropriate
methods which cannot be ignored. Therefore, it should be protected and the studies on the plant should
be increased.

KEYWORDS
Key Words: Rheum ribes, Erzurum, Medicinal Plants, Treatment, Pharmacology

Corresponding Author: DR. BANU KADIOLU, banu250@hotmail.com

1
DOU ANADOLU TARIMSAL ARATIRMA ENSTTS MDRL

1202
 Poster Session 9
Submission ID: 1139

MEDICAL AND AROMATIC PLANT PRODUCTION AND FOREIGN

TRADE FROM 2000 TO TODAY

1 1 1 2
MKREMIN TEMEL , A. BIRCAN TINMAZ , MUSTAFA ZTRK , ORHAN GNDZ

ABSTRACT
This study is a part of the Turkish Medicinal and Aromatic Plant Sector Analysis Project
supported by General Directorate of Agricultural Research and Politics. This project aimed to give
detailed information about the production, foreign trade amounts and changes of medicinal and
aromatic plants from the year 2000 onwards. The research covers all medicinal and aromatic plants
and takes place by using secondary data. Secondary data were obtained from TUIK records (foreign
trade records) by screening, filtering and grouping methods and subjected to descriptive statistics.
According to the findings, about 20 kinds of medicinal and aromatic plants are cultivated in the field
of 1.3 million decares, and black tea, redbibber, poppy, cumin, mint, thyme, oil rose and anise are in
the first place in terms of production amount. From 2000 to 2015, production sites increased by about
50%. On the other hand, more increases in the production quantities have occurred. There are dozens
of plants in the foreign trade of medicinal and aromatic plants. Some of these plants are considered
medicinal and aromatic plants, while they are found in industrial plants or oil seeds. Foreign trade of
medicinal and aromatic plants was realized with export of 280 million dollars and import of 254
million dollars in 2015. The most important plants in export are thyme, poppy, laurel, tea, anise,
caraway, sage, mahlep, redbibber and herbal teas. Thyme is in the first place with 25% share in
exports. Thyme exports have increased by 300% compared to the past, reaching 56 million dollars and
approximately half of them are realized in the Aegean Free Zone. Despite the increase in exports of
many plants, red pepper, hemp, rosemary, peppermint, paint plants have decreased in exports. The
most important plants in import are coffee, tea, linen, black pepper, carob, cumin, cumin, hops. Coffee
constitutes 61% of imports. There are also many reeksports such as thyme, laurel, cumin, coffee, sage
and carob. As a result, medical and aromatic plant production areas have increased, exports have
increased, while imports have also increased. The foreign trade surplus is 25.3 million dollars while
the coffee account includes 121.5 million foreign trade deficit.

KEYWORDS
medical and aromatic, 2000's, production, foreign trade

Corresponding Author: MKREMN TEMEL, mukremintemel@gmail.com

1
ATATRK BAHE KLTRLER MERKEZ ARATIRMA ENSTTS MDRL
2
NN NVERSTES, BATTALGAZ MESLEK YKSEK OKULU

1203
 Poster Session 9
Submission ID: 1141

TRADITIONAL USES OF MEDICINAL AROMATIC PLANTS

OBTAINED FROM FOREST AREAS

1 1
GZDE SEZGIN , GLIN ZER

ABSTRACT
In recent years, there has been an increasing demand for wood raw materials produced from
ornaments in the world market as well as for non-wood forest products. This increase is important not
only for the purposes but also for the needs of the people. Turkey, which contains 20% of herbal
products used for various purposes in the world, while many of the export of medicinal and aromatic
plant is located in an important position in imports. In addition, our country is the ecological potential
and one of the world's few countries. As in the rest of the world, the use of medicinal and aromatic
plants found in natural flora in our country has become a part of our traditional cultural richness from
centuries ago. For example, the treatment of people, food, tea, spices, dyes, treatment of animal
diseases, resin, gum, benefiting from essential oils and use in the beverage-cosmetics industries. The
transfer of medicinal aromatic plants that are used throughout the history for different purposes and
their utilization patterns to future generations is very important in terms of not losing this information.
In this study made for this purpose, the history of medicinal aromatic plants, the potential in the
country, the traditional usage patterns of local people have been investigated.

KEYWORDS
Medicinal aromatic, traditional usage, forest product

Corresponding Author: GZDE SEZGN, gozdeakcinar@gmail.com

1
MARMARA ORMANCILIK ARATIRMA ENSTTS MDRL

1204
 Poster Session 9
Submission ID: 1142

THE USE OF COMPLEMENTARY AND ALTERNATIVE MEDICINE

IN OUTPATIENTS OF ANKARA ANKAYA CEVIZLIDERE FAMILY
HEALTH CENTER: PRELIMINARY RESEARCH

1 2
ZLEM AYNAOLU HAKVERDI , ALEV NDER

ABSTRACT
The aim of this study is to examine the types and the use of Complementary and Alternative
Medicine (CAM), as well as the factors affecting the use of CAM in ankaya Cevizlidere Family
Health Center, Ankara, Turkey. In the study, 75 respondents were selected randomly from the Family
Health Center and questionnaires were applied. The results show that 65 % of the respondents use
different types of CAM. Socio-demografic characteristics did not show any significant effect on the
overall use of CAM.

KEYWORDS
Questionnaire, CAM, Alternative Medicine, Health

Corresponding Author: ZLEM AYNAOLU HAKVERD, aynaogluh@ankara.edu.tr

1
ANKARA NVERSTES, THSK ANKAYA TSM CEVZLDERE ASM
2
ANKARA NVERSTES, ECZACILIK FAKLTES

1205
 Poster Session 9
Submission ID: 1144

EFFECTS OF PHYTOCHEMICALS ON AGING

1 1 1 1
BURCU DENIZ GNE , NILFER ACAR TEK , GAMZE AKBULUT , HATICE BLKBAI

ABSTRACT
Aging is defined as a genetic physiological process associated with morphological and
functional changes in cellular and extracellular components aggravated by injury throughout life and
resulting in a progressive imbalance of the control regulatory systems of the organism, including
hormonal, autocrine, neuroendocrine and immune homeostatic mechanisms. Aging includes a
reduction in strength, endurance, the speed of reaction, agility, basal metabolism, sexual activity and
hearing acuity. Aging is primarily caused by external or environmental factors that inflict cellular
damage, ultimately leading to organ damage and death. The exact mechanisms underlying the aging
process are not well understood, but increasing evidence shows that aging is highly associated with the
chronic increase in reactive oxygen species (ROS), accumulation of a low-grade proinflammatory
phenotype and reduction in age-related autophagy. Also, aging in humans is associated with a greatly
increased incidence of a number of degenerative diseases including cardiovascular disease, Type 2
diabetes, cancer and Alzheimer's disease. Both aging and chronic diseases are highly associated with
increased metabolic and oxidative stress, elevated chronic, low-grade inflammation, and accumulated
DNA mutations as well as increased levels of its damage. All these factors may play an important role
in the progress of aging. It was expressed that some phytochemicals present in the foods are antiaging
molecules, and dietary intake of these compounds can promote health and extend lifespan. These
effects can be associated with multiple mechanisms, including reducing oxidative stress, suppressing
low-grade chronic inflammation, inducing autophagy, as well as regulating several important
molecules involved in promoting mitochondrial function and energy homeostasis. Resveratrol,
epicatechin, quercetin, curcumin and epigallocatechin gallate (EGCG) are anti-aging effective
phytochemicals. It was reported that resveratrol improved insulin resistance, blood flow, and various
cardiovascular events, as well as decreased oxidative stress and inflammation, may point to a
promising antiaging action of this compound, given that cardiovascular disease is a major cause of
age-related morbidity and mortality in humans. Dietary intake of epicatechin can improve blood vessel
function, insulin sensitivity, blood pressure, and inflammation, all of which could be associated with
the aging process. Quercetin, curcumin and EGCG are thought to play an important role in preventing
aging, with antioxidant and anti-inflammatory properties preventing oxidative stress and
inflammation. Additionally, other phytochemicals such as fisetin, butein, phloridzin, kaempferol,
glaucarubinone and garlic extract, which contains s-allycisteine, s-allymercaptocysteine, allicin and
diallosulfides were also found to be anti-aging effects in vitro studies. As a result, it can be said that
phytochemicals are anti-aging effects due to the antioxidant, anti-inflammatory and other properties.
But there is a need for more studies on people to clarify these effects.

KEYWORDS

Corresponding Author: BURCU DENZ GNE, brc-dnz@hotmail.com

1
GAZ NVERSTES SALIK BLMLER FAKLTES

1206
Phytochemicals, anti-aging

1207
 Poster Session 9
Submission ID: 1145

SEASONAL EVALUATION OF MEDICINAL AND AROMATIC PLANT

CONSUMPTION (SAMPLE OF ERZURUM PROVINCE)

1 1
DR. BANU KADIOLU , DR. SIBEL KADIOLU

ABSTRACT
Abstract: Turkey is a country where there are around 12.000 plant species whose 1/3 is
endemic and 30% of this rate is aromatic. Among rich variety of plants, medicinal and aromatic herbs
have a significant place and it is known that in Turkey about 500 plant species are used as medicine by
the general public. In this study that intends to detect knowledge and consumption habits on medicinal
and aromatic herbs of the consumers in Erzurum, sample size is determined as 384. Questionnaire is
distributed to the neighborhoods proportionally. The main material of the research is the questionnaire
data conducted with the consumers face to face. In the research results, consumers' medicinal and
aromatic plant consumption habits are given by frequencies, percentages and cross tables depending
on the seasons. The medicinal and aromatic plant consumption habits of the consumers vary
depending on the season. In summer the most commonly used plant are coriander (Coriandrum
sativum), mint (Mentha sp.) and nettle (Urtica sp.). These plants are used as spices, sweetening and for
digestive system problems. During the winter months, cinnamon (Cinnamomum sp.), linden (Tilia sp.)
and rosehip (Rosa sp.) are used for treating influenza and cold, strengthening of the immune system
and the fulfillment of the need for vitamins.

KEYWORDS
Key words: Medicinal and aromatic plants, consumer habits, seasonal consumption

Corresponding Author: DR. BANU KADIOLU, banu250@hotmail.com

1
DOU ANADOLU TARIMSAL ARATIRMA ENSTTS MDRL

1208
 Poster Session 9
Submission ID: 1146

VOLATLE OL FOREGN TRADE FROM PAST TO TODAYS

1 1 1 2
MKREMIN TEMEL , A. BIRCAN TINMAZ , MUSTAFA ZTRK , ORHAN GNDZ

ABSTRACT
This study is a part of the Turkish Medicinal and Aromatic Plant Sector Analysis Project
supported by General Directorate of Agricultural Research and Politics. This project aimed to give
detailed information about the production, foreign trade amounts and changes of volatile oil. This
study covers all volatile oil and takes place by using secondary data. Secondary data were obtained
from TUIK records (foreign trade records) by screening, filtering and grouping methods and subjected
to descriptive statistics. According to findings, volatile oil foreign trade is realized as $ 34 million
export and $ 27 million import in 2015. Exports were 11.6 million and imports were 5.3 million
dollars in 2002. The most important essential oils in export are rose, thyme, stearopten, orange. In
export, rose ranks first with 36% share. Rose exports was 8 million dollars in 2002 and 11 million
dollars in 2015. France is the most important country with a share of 61% in volatile oil exports.
Important essential oils in import are mint, orange, lemon, other citrus fruits, lavender. Mint is in the
first place with a share of 14%. It reached $ 2.8 million in 2015, while it was $ 1.2 million in 2002.
Important countries in importing essential oil are India, Germany and USA. Resinoids, by-products,
oil resins and distilled water and concentrates of essential oils are also subject to foreign trade. As a
result, exports are increasing, imports are increasing at the same rate, and there is a steady increase in
foreign trade.

KEYWORDS
essential oils, past, present, foreign trade

Corresponding Author: MKREMN TEMEL, mukremintemel@gmail.com

1
ATATRK BAHE KLTRLER MERKEZ ARATIRMA ENSTTS MDRL
2
NN NVERSTES, BATTALGAZ MESLEK YKSEK OKULU

1209
 Poster Session 9
Submission ID: 1149

ETHNOBOTANICAL USAGE OF SOME PLANT TAXA SPECIES

NATURALLY GROWING IN ESKIEHIR

1 1 1 2 1
KURTULU ZG , OKAN SEZER , SMHAN POTOLU ERKARA , DERVI ZTRK , MER KORAY YAYLACI ,
1
ONUR KOYUNCU

ABSTRACT
Eskiehir, with its approximately 220 endemic and over 1300 plant taxa, is one of the most
important geographical regions of the Central Anatolia region in terms of floristic structure. Because
of having neighbors in the north side such as Bolu and Bilecik where the European-Siberian
phytogeographic region plant groups are dominate; the presence of large Central Anatolian steeps in
the east, west and south side of the city where the Iranian-Turan phytogeographic region elements are
dominate and having microclimate region around the Sakarya basin where the plant groups of
Mediterranean phytogeographical region are dominate, Eskiehir hosts the plants of three kinds of
phytogeographical regions. The usege of plants for different purposes by people in a region where the
plant diversity is so high, is also quite common. Asteraceae (127 taxa) ; Fabaceae (94 taxa);
Lamiaceae (83 taxa); Brassicaceae (70 taxa) and Caryophyllaceae (51 taxa) are in the top five in terms
of the number of taxa which grown naturally in Eskiehir. When considered in terms of ethnobotonical
usage, Lamiaceae (27 taxa) ; Asteraceae (21 taxa); Apiaceae (7 taxa); Rosaceae (5 taxa) and Fabaceae
(4 taxa) are mostly used families. In this study which is based on both field and literature studies,
ethnobotonical usage, usage methods, endemism, distribution patterns and phytogeographical
characteristics of some plant taxa which are naturally grown in Eskiehir have been determined. It has
been found that 65 different taxa belonging to 50 genus from 35 different families have been used for
ethnobotonical purposes. 11.4% of these plants which are used by local people for ethnobotonical
purposes are member of Mediterranean phytogeographic region element; 10.2% Iran-Turan
phytogeographic region element and 9.23% Europe-Siberia phytogeographic region element. In
addition, 4,61% of the plant taxa used for ethnobotanical purposes are endemic plant taxa.

KEYWORDS
Ethnobotany, Medical Plant, Biodiversity, Eskiehir, Turkey

Corresponding Author: MER KORAY YAYLACI, omerkoray@gmail.com

1
ESKEHR OSMANGAZ NVERSTES, FEN EDEBYAT FAKLTES, BYOLOJ BLM, ESKEHR
2
ESKEHR OSMANGAZ NVERSTES, MAHMUDYE ATILIK MESLEK YKSEK OKULU, ESKEHR,
TURKEY

1210
 Poster Session 9
Submission ID: 1152

MEDICINAL AND AROMATIC PLANTS OBTAINEDAS NON-WOOD

FOREST PRODUCT

1 1 1
A. BIRCAN TINMAZ , MKREMIN TEMEL , YASIN ZDEMR

ABSTRACT
This study aimed to give detailed information about quantities and changes of medicinal and
aromatic plants obtained as forest side product by years. The data are compiled from the records of the
Ministry of Forestry and Water Affairs. According to findings, there are close to 30 medicinal and
aromatic plant varieties collected as non-wood forest product, and laurel leaf, thyme and carob are in
the first order. The amount of laurels, goat horns, lime increased when compared in the 1990's, while
the amount of thyme, laden and steed decreased. It is seen that plants such as cantoron, cauliflower,
lavender are obtained from forest areas and the quantities are not recorded properly in records.

KEYWORDS
medicinal and aromatic, non-wood

Corresponding Author: A. BRCAN TINMAZ, ahmettinmaz@gmail.com

1
ATATRK BAHE KLTRLER MERKEZ ARATIRMA ENSTTS MDRL

1211
 Poster Session 9
Submission ID: 1155

ANTIOXIDANT ACTIVITY OF NEW APPLE CULTIVAR: MALUS

COMMUNIS L. (PIRAZIZ APPLE)

1 1
AYTA GDER , SEVIM FT YEGN

ABSTRACT
Malus communis L., local cultivar is known Piraziz Apple by the local people, grown in
Piraziz (Giresun). This cultivar was patented by the Turkish Patent and Trademark Office (No: 204
Patent s Owner: Piraziz Ziraat Odas). According to the literature, beginning of the flowering, full
flowering and harvesting period were May 25-30, 6th June and 15th November in 2011. On the other
hand, these periods were determined as April 25-30; 6th May and 15th October in 2012. 160 days pass
from the full flowering to the harvest. Apple samples were harvested in November, 2016. In this study,
the antioxidant capacity of this cultivar s apple was assessed by using different antioxidant test
methods such as hydrogen peroxide scavenging activity, ferric reducing antioxidant power capacity
(FRAP) at the 100 g/mL concentration, DPPH radical scavenging activity, metal-chelating activity at
the 100 g/mL concentration, total phenol content (TPC), and total flavonoid content (TFC). These
values were determined as 686.18 g/mL (SC50), 6.44 (%), 273.12 g/mL (SC50), 19.03 (%), 16.21
mg GAE/g extract and 12.91 mg CAE/g extract, respectively. Buthylated hydroxy anisole (BHA),
buthylated hydroxy toluene (BHT) and -tocopherol (TOC) were used for comparison of the data as
standard antioxidant compounds. When antioxidant results of apple compare with standards, we can
say that this apple cultivar dont show effective antioxidant activity as well as standard compounds.
Although Piraziz Apple are used antidiabetic agent by the local community as ethnopharmacological
material, it has no effective antioxidant activity. So this cultivar is generally used as antidiabetic agent.
According to obtained results, we cay say that these activities can be related to active components in
the Piraziz Apple. Consequently, this new cultivar s biological properties can be investigated
profoundly by the researchers because of the ethnopharmacological and ethnobotany properties.

KEYWORDS
Malus communis L., Piraziz Apple, Antioxidant activity, total phenol and flavonoid contents.

Corresponding Author: AYTA GDER, aytac.guder@giresun.edu.tr

1
GRESUN NVERSTES

1212
 Poster Session 9
Submission ID: 1156

SOME AROMATIC PLANTS OF PHRYGIAN VALLEY

(ASTERACEAE, LAMIACEAE, APIACEAE)

1 1 2 1 3 1
OKAN SEZER , ATILA OCAK , MER KORAY YAYLACI , KURTULU ZG , DERVI ZTRK , ONUR KOYUNCU

ABSTRACT
Turkey is among the leading countries of the world in terms of its floristic diversity. Turkey,
which has approximately 12,000 plant taxa has more higher floristic diversity than many other
countries. Turkey is also among the few rare places on the World because of its high endemism ratio
(31%). Moreover its gene center of the many plant taxa. Many plants that be in flora of Turkey have
been used for traditional medicine purposes locally and globally. Numerous plant taxa belonging to
various families which naturally distributed in our country are also used for commercial medicine and
pharmacy fields. When family-based distributions of medicinal and aromatic plants in our country are
examined, it is observed that plant taxa belonging to families such as Asteraceae, Lamiaceae and
Apiaceae constitute the majority of these plants. In this study, Asteraceae, Lamiaceae and Apiaceae
taxa of Phrygian Valley where located at the junction point of the Eskiehir, Ktahya and Afyon
provinces and also getting popular in terms of ecotourism in recent years were determined. A total of
107 taxa belonging to the family of Asteraceae that includes 104 species and 3 subspecies were
identified. Also 64 plant taxa (62 species and 2 subspecies) belonging to 20 genera from Lamiaceae
and 37 plant taxa (35 species, 1 subspecies and 1 variety) belonging to 24 genera from Apiaceae were
determined. Totally 208 plant taxa from these 3 families were identified from study area. 26 of these
are endemic and the endemism rate is 12.5%. 43 of these taxa are Irano-Turian, 29 Euro-Siberian and
21 Mediterranean. 115 of these 208 taxa are multiregional or floristic region are unknown. This study
aimed to determine the floristic diversity of Asteraceae, Lamiaceae and Apiaceae, which spread in the
Phrygian valleys, and thus to guide the further studies which will be carried out on the taxa belonging
to this family.

KEYWORDS
Phrygian Valley, Medicinal and Aromatic Plant, Biodiversity, Turkey

Corresponding Author: DERV ZTRK, dervisozturkk@gmail.com

1
ESKEHR OSMANGAZ NVERSTES, FEN EDEBYAT FAKLTES, BYOLOJ BLM, ESKEHR
2
ANADOLU NVERSTES, ECZACILIK FAKLTES, FARMASTK BOTANK ANABLM DALI, ESKEHR,
TURKEY
3
ESKEHR OSMANGAZ NVERSTES, MAHMUDYE ATILIK MESLEK YKSEK OKULU, ESKEHR,
TURKEY

1213
 Poster Session 9
Submission ID: 1157

DETERMNATON OF SOME HEAVY METAL CONCENTRATONS

OF SAGE TEA WTH FAAS

1 1 1
FEVZI KILIEL , HACER SIBEL KARAPINAR , AYE UUZ

ABSTRACT
Herbal teas are blends based on the plant's roots, root hairs, branch shoots, aromatic parts of
leaves, flowers, shells, fruits, or seeds that are prepared by drying aromatic parts in boiling water.
Although plant teas are known to have many benefits in terms of health, they are an important threat to
human life if they are exposed to some contamination due to misuse or overuse or uncontrolled
production. A significant proportion of these exposures constitute heavy metals. The living
environment of the plants is polluted with many harmful substances which arise from various sources.
Factors such as the development of industry and increasing traffic, pesticides, industrial and household
waste increase the heavy metal pollution. Considering the studies evaluating herbal teas in terms of
heavy metal contents, it is seen that in herbal teas there are elements naturally found in plants such as
Cu, Co, Zn, Mn, Fe and necessary for human health. However, some metals, such as Ni, Pb, Cd, As,
Hg, which are poisonous, appear to be present when they reach certain levels (1,2). To prevent heavy
metal accumulation and health problems caused by it, it is important to avoid heavy metal
contamination in stages such as plant collection, production, packaging, storage and storage. If it is
thought that the people use these unconsciously and uncontrollably, it is very important to make legal
regulations and audits in this area. In this study, adequate quantities of sage tea (salvia officinalis)
plants offered for sale in markets, and herbalist in Karaman were taken in sufficient quantities and
analyzed by appropriate drying, milling and dissolution processes. The samples were prepared to be 2
parallel for each sample and were solutioned by wet burning method. For this purpose, one gram of
the powdered sample washed and dried in a suitable manner is precisely weighed 16 ml HNO3 (65%,
w/w) and 4 ml HClO4 (70-72%, w/w) are added to it and the solution is slowly heated in the drawer
for about 5-6 hours. The heating process close to the end of the acids is cut off and the solutions are
cooled. Then 5 ml H O (30%, w/w) was added and heating was continued until clear liquid was
obtained. Heating was stopped when clear liquid was formed, and the solutions were allowed to cool.
Cooling solutions were filtered through blue band filter paper and 15 ml of the obtained solutions were
mixed with distilled water to prepare the analyzed. The concentrations of the determined elements
were determined by Flame Atomic Absorption Spectrometry. The amounts of Co, Ni, Cu, Zn, Cd, Mn,
Mg, Fe and Ca were determined in all of the foods determined according to the results. Cr was not
detected in the samples.When the results were evaluated Co, Ni, Fe and Ca were found to be above the
determined limits. Co, Ni, Fe element concentrations are above the normal range but below the toxic
limits. It should be noted that these foods can easily be contaminated due to factors such as the
physical and chemical structure of the soil, agricultural activities, storage and packaging conditions.
Moreover, it is important to consciously consume and make the necessary quality controls not to
adversely affect public health.

Corresponding Author: HACER SBEL KARAPINAR, sibelkarapinar@kmu.edu.tr

1
KARAMANOLU MEHMETBEY NVERSTES

1214
KEYWORDS
Sage tea, Heavy metal, FAAS, Karaman, Turkey.

1215
 Poster Session 9
Submission ID: 1158

LAMIACEAE TAXA OF GYPSUM AND MARL SOILS IN ESKIEHIR

1 2 2 2
DERVI ZTRK , ATILA OCAK , OKAN SEZER , ONUR KOYUNCU

ABSTRACT
The investigation area covers gypsum and marl soils reside in the border of Eskisehir. It
occurs in the B3 square according to the grid system of P.H. Davis. To investigate the flora, 1750
specimens have been collected during the field seasons of 2012- 2015. The floristic list follows the
APG III. At the end of identifications of the specimens 47 taxa belonging to Labiate family have been
determined. The endemism ratio of the areas is 17.5 %.

KEYWORDS
B3 square, Eskiehir, gypsum, marl, flora

Corresponding Author: OKAN SEZER, oksezer@ogu.edu.tr

1
ESKEHR OSMANGAZ NVERSTES MAHMUDYE ATILIK MESLEK YKSEK OKULU, ESKEHR,
TRKYE
2
ESKEHR OSMANGAZ NVERSTES, FEN EDEBYAT FAKLTES, BYOLOJ BLM, ESKEHR

1216
 Poster Session 9
Submission ID: 1160

INVESTIGATION OF EFFECTS ON RIPENING OF DIFFERENT

TYPES OF SPICES USED IN KASHAR CHEESE PRODUCTION

1 2
ZEKAI TARAKI , ENGIN AYDIN

ABSTRACT
Investigation of Effects on Ripening of Different Types of Spices Used in Kashar Cheese
Production* Zekai TARAKI1, Engin AYDIN2 1Department of Food Engineering, Agricultural
Faculty, Ordu University, Ordu, Turkey 2Department of Cooking, Vocational College, Giresun
University, Giresun, Turkey *This research was supported with number TF-1624 by ODU-BAP.
1zetarakci@hotmail.com In this study, six types of Kashar cheese, one of them is control sample that
was produced without adding any spices; the others samples were processed to cheese by adding dead
nettle, mint, lemon balm, arugula and parsley as 0.5% according to the milk used. Then, all cheeses
were vacuum-packed and ripened at 71C during 3 months and dry matter, fat, pH, titratable acidity,
salt, total protein, ripening rate, nonprotein nitrogen rate (NPN), amino nitrogen rate, textural
properties and sensory analyses were performed by taking cheese samples during the 2nd, 30th, 60th
and 90th days of ripening. The results were compared in terms of the types of cheese and ripening
period, statistically. The values of adhesiveness, springiness and resilience parameters of texture
profile analyses and also the values of structure-texture parameters of sensory analyses were founded
importance difference. The values of fat and the values of springiness parameters of texture profile
analyses were determined statistically significant. All of the other parameters were obtained
statistically differences. As a result of sensory evaluation made by panelists, ripened kashar cheeses
had more acceptability than fresh kashar cheeses. Keywords: Kashar cheese, spices, ripening criteria

KEYWORDS
Kashar cheese, spices, ripening criteria

Corresponding Author: ZEKA TARAKI, zetarakci@hotmail.com

1
ORDU NVERSTES
2
GRESUN NVERSTES

1217
 Poster Session 9
Submission ID: 1161

ELEMENTAL COMPOSITION DIVERSITY OF SELECTED WILD

EDIBLE PLANTS FOR ALTERNATIVE NUTRITION

1 1 1 1
M. KADRI BOZOKALFA , BLENT YAMUR , DURSUN EIYOK , TANSEL KAYGISIZ AIOUL

D
ABSTRACT
Vegetables and fruits in general are an important category in food based approaches a sources

TE
of multiple micronutrients. In addition to meeting nutrient intake levels, greater consumption of fruits
and vegetables is associated with reduces risk of developing chronic diseases. Within this group,
gathered wild vegetables deserve greater attention several research show how they are easily
accessible, culturally accepted and inexpensive sources of vitamins and minerals to many rural
population groups. Diversity and number of examples from different region indicate their importance
EN
at an aggregated level. The wild edible plants are consumed in many different ways but mostly eaten
raw as a salad. However, widespread knowledge of wild edible plants not provide much information
on their nutritional significance and their diversity. The objective of the present work was to evaluate
variability for dry matter, protein and mineral composition of nutritionally important and widely
ES
consumed wild edible plants in Aegean region of Turkey. The plant material comprises 17 edible
plants collected from naturally found and widely distributed in the experimental area of Horticulture
Department of Ege University. In order to reduce environmental and edaphic factors on the
composition of the plant samples and visualize genotypic differentiation, the plant material collected
from the a total 500 m2 area where the soil that has not been cultivated for a long time, furthermore
PR

not use any chemicals such as fertilizer and pesticide in collection site. A total 17 edible wild species
Lactuca serriola L., Capsella bursa-pastoris L. Medik., Malva sylvestris L., Papaver rhoeas L., Urtica
diocia L., Erodium cicutarium (L.) L'Herit., Chondrilla juncea L., Stellaria media L. Rumex patientia
L., Taraxanum officinale, Allium ampeloprasum L., Plantago lagopus L., Sonchus oleraceus L.,
Daucus carota, Sinapsis arvensis L., Mentha pulegium L., Portulaca oleraceae L. were evaluated for
dry matter, protein, N (nitrogen), P (phosphorus), K (potassium), Ca (calcium), Mg (magnesium)
T

composition. The data were subject to analysis of variance, and a Pearson correlation test was used to
determine the correlations between dry matter, protein content and N, P, K, Ca, Mg compositions.
O

Principal component analysis was performed on the result of examine compositions and the factor
loadings, eigenvalues and percentage of cumulative variance were calculated, the patterns of
N

relationships among nutritive element were shown two-dimension scatter plot. Dry matters, protein
and mineral elements were found to vary widely depending on species. According to the results the
wild edible species dry matter content ranged from 8.65-20.11% in the edible parts of plant, and the
highest protein (29.94%) and N (4.79 mg 100 g-1) values were observed in C. bursa-pastoris L.
Medik., P content ranged between 0.19-0.37 mg 100 g-1, where P. rhoeas showed the highest. K was
found in the range of 2.24 to 4.24 mg 100 g-1 respectively, for M. pulegium L. and P. oleraceae L. Ca
was present good amount in U. diocia L. 3.47 mg 100 g-1 while other species in the range of 0.64-2.64
mg 100 g-1. Similarly, other micro minerals including Mg (0.14-0.43 mg 100 g-1) and Na (0.04-0.86
mg 100 g-1) were observed in the selected plant species. Multivariate analysis revealed considerable

Corresponding Author: M. KADR BOZOKALFA, mehmet.kadri.bozokalfa@ege.edu.tr

1
EGE NVERSTES ZRAAT FAKLTES

1218
variation for the most of concentration. The principal component (PC) analysis explained that 81.49%
of total variation accounted for three PC axis. The first axis was mainly related to variation in protein,
N and P compositions. The second axis was mainly concerned with dry matter, K, Ca, and Na, the
third axis related to Mg. The data reveal that selected wild plant provide significant nutrition and
genetic background of species can play role in the nutritional value.

KEYWORDS
Nutritional value, minerals, wild edible plants, principal component analysis

1219
 Poster Session 9
Submission ID: 1162

INVESTIGATION OF NATURAL ADVERTISING OF SOME MEDICAL

AND AROMATIC PLANTS IN THE GAP OF PEANUT PINE
PLANTATION

1 1 1 2
A. BIRCAN TINMAZ , MKREMIN TEMEL , MUSTAFA ZTRK , MEHMET ZDEMR

ABSTRACT
There are hundreds of medical and aromatic plants in our country that are collected from
nature and offered for sale or exported. In the process of nomination to the Mediterranean model forest
network being established, work has been initiated to improve forest-peasant relations and to promote
the cultivation of non-forest products in forest openings and to increase the participation of villagers in
the system. In this study, organic farming possibilities of some medicinal and aromatic plants (Sage,
Rosemary, Thyme, Lavender and Laurel) were investigated in the openings of pistachio pine
plantation of Armutlu peninsula. At the end of the project, the optimal planting time and location of
the plants to be planted in different ways (shaded view, sunny view, uninjured area) and at different
times (autumn, winter, spring) were determined. Experiments that were established separately for each
plant, 3 replications were set up in random parcel trial design in random blocks. The results were
subjected to analysis of variance and significant averages were evaluated by the Lsd test. With the
study, the new plant, Sage, Rosemary, Lavender planted in the forest openings, has been adapted to
the newly formed ecosystem and it has come to the conclusion that the breeding can be done in such
areas. Competition with other forest bushes and weeds was found to be weak due to the herbaceous
structure of the Istanbul Thyme used in the study. It is observed that these areas where defnesin is
planted are not suitable for uncontrolled cultivation and that their development is not good and they
can not take place in the ecosystem. The study is a first for Turkey and a model for breeding.

KEYWORDS
Medical plant, organic farming, lavender, thyme, rosemary, sage, Yalova

Corresponding Author: A. BRCAN TINMAZ, ahmettinmaz@gmail.com

1
ATATRK BAHE KLTRLER MERKEZ ARATIRMA ENSTTS MDRL
2
MARMARA ORMANCILIK ARATIRMA ENSTTS MDRL

1220
 Poster Session 9
Submission ID: 1163

PHYTOCHEMICAL ANALYSIS OF DIFFERENT PARTS OF SALVIA

PAHYSTACHYS BY USING LC-MS/MS

1 2 3 4
MUSTAFA ABDULLAH YILMAZ , ELIF VARHAN ORAL , ABDULSELAM ERTA , HAVA BATIBAY , MEHMET
5 3 6 6 1 4
FIRAT , ESRA YARI , HILAL SARUHAN FIDAN , HSEYIN ALKAN , HAMDI TEMEL , BERRIN ZIYADANOULLARI

ABSTRACT
Salvia L. genus is member of lamiaceae family which exists approximately 220 genus and
4000 species on the world. Although it shows most natural distribution in Turkey and Mediterranean
region on the world, almost it can be grown in all habitat types and heights. Salvia L. genus is
represented about 89 species in Turkey and 45 of them are endemic. Salvia species are generally
known for their multiple pharmacological effects including their antibacterial, antiviral, antioxidative,
antimalarial, anti-inflamatory, antidiabetic, cardiovascular, antitumor, and anticancer. In this study,
secondary metabolite profile (37 phytochemicals including 15 Phenolic acids, 17 flavonoids, 3
nonphenolic organic acids, 1 phenolic aldehyde and 1 penzopyrane) of ethanol extracts of different
parts (stems, leaves, flowers, roots and mixed parts) of Salvia pahystachys was determined with LC-
MS/MS. LC-MS/MS method validation was developed for the qualitative and quantitative analysis of
37 phytochemicals. The LC-MS/MS studied showed that S. pachystachys was including high amount
hesperidin, caffeic acid, fumaric acid, malic acid, apigenin and especially rosmarinic acid.

KEYWORDS
Salvia pahystachys, LC-MS/MS, Phenolic Content

Corresponding Author: ELF VARHAN ORAL, evarhan@dicle.edu.tr

1
DCLE UNVERSTY, DEPARTMENT OF PHARMACEUTCAL TECHNOLOGY, FACULTY OF PHARMACY,
DYARBAKIR
2
DCLE UNVERSTY, FACULTY OF PHARMACY, DEPARTMENT OF ANALYTCAL CHEMSTRY, DYARBAKIR
3
DCLE UNVERSTY, FACULTY OF PHARMACY, DEPARTMENT OF PHARMACOGNOSY, DYARBAKIR
4
DCLE UNVERSTY, FACULTY OF SCENCE, DEPARTMENT OF CHEMSTRY, DYARBAKIR
5
YZNC YIL UNVERSTY, FACULTY OF EDUCATON, DEPARTMENT OF BOLOGY, VAN
6
DCLE UNVERSTY, FACULTY OF PHARMACY, DEPARTMENT OF BOCHEMSTRY, DYARBAKIR

1221
 Poster Session 9
Submission ID: 1164

MEDICINAL AND AROMATIC LAMIACEAE TAXA THAT

NATURALLY DISTRIBUTED IN PAZARYER (BLECK) AND ITS
ENVIRONS

1 2 3 3 3
BEYZA GLER KOCAMAN , MER KORAY YAYLACI , KURTULU ZG , HAKAN ENTRK , OKAN SEZER ,
4 3 3
DERVI ZTRK , ONUR KOYUNCU , SMHAN POTOLU ERKARA

ABSTRACT
The Lamiaceae family, which has an important place among medicinal and aromatic plants, is
represented by about 250 genera and 7000 species in the world. Because of the aromatic oils, essential
oils and a wide variety of secondary metabolites they have, great deal of these have been using as
spice, food and medical purposes in our country and in the world since ancient times. Turkey is among
the Lamiaceae-rich regions of the world. Moreover, Turkey is the gene center of many Lamiaceae taxa
and is host in a large number of endemic taxa. Lamiaceae is among the largest families of The flora of
Turkey and it is represented with near 574 species from 45 genera between about 12,000 taxa of
Turkey flora. In this study, Lamiaceae specimens have been collected through the Pazaryeri (Bilecik)
and its environs between 2013-2014 years. Totally, 50 Lamiaceae taxa (45 species, 5 subspecies)
belonging to 18 genera were identified from study area. 13 of these taxa are Irano-Turanian, 8 Euro-
Siberian and 6 Mediterranean. Other 23 taxa are multiregional or their distribution unknown. 9
endemic taxa (7 LC and 2 NT) determined from study area and endemism ratio 18%. This study aims
to determine the Lamiaceae taxa which spread naturally in the vicinity of the Pazaryeri (Bilecik) and
so reveal the medicinal and aromatic dimensions of these taxa.

KEYWORDS
Pazaryeri, Bilecik, Biodiversity, Medicinal and Aromatic Plants, Turkey

Corresponding Author: ONUR KOYUNCU, okoyuncu@ogu.edu.tr

1
BLECK EVRE VE EHRCLK L MDRL, BLECK, TRKYE
2
ANADOLU NVERSTES, ECZACILIK FAKLTES, FARMASTK BOTANK ANABLM DALI
3
ESKEHR OSMANGAZ NVERSTES, FEN EDEBYAT FAKLTES, BYOLOJ BLM
4
ESKEHR OSMANGAZ NVERSTES, MAHMUDYE ATILIK MESLEK YKSEK OKULU

1222
 Poster Session 9
Submission ID: 1165

EVALUATION OF THE ANTIOXIDANT CAPACITY AND PHENOLIC

CONTENT OF JUNIAEA MESOPOTAMICA EXTRACT OBTAINED
WITH DIFFERENT SOLVENTS.

1 1 1 1
ADEM NECP , MESUT IIK , ABDURRAHMAN AKDA , SMAIL KOYUNCU , ABDULLAH AYKANAT, REAT
1
DKME

ABSTRACT
Antioxidant plays an important role in inhibiting and scavenging free radicals, thus, providing
protection to human against infection and degenerative diseases (Ansari, 2013). Now the modern
research is directed towards Natural antioxidants from the herbal plants due to safe therapeutic. In
the present paper we have investigated antioxidant activity of extracts from Juniaea mesopotamica for
its free radical scavenging activity by adopting various in vitro methods. The methanol and buthanol
extracts of Juniaea mesopotamica exhibited significant antioxidant activities determined by different
assays. This study shows that methanol and buthanol extracts of Juniaea mesopotamica have higher
antioxidant activities than dichlorometane and hexane extract. Moreover, this plant showed
antioxidant properties close to the standards. This suggests that Juniaea mesopotamica extract is a
potential source of natural antioxidants, which could be added to dietary supplements to help prevent
oxidative stress.

KEYWORDS
Juniaea mesopotamica, Antioxidant, free radicals

Corresponding Author: MESUT IIK, misik@harran.edu.tr

1
HARRAN NVERSTES

1223
 Poster Session 9
Submission ID: 1166

DETERMINATION OF ANTIOXIDANT PROPERTIES OF DRY ROSE

TEA

1 1
DILAY KART , ZLEM ZLEM AINDI

ABSTRACT
ABSTRACT Medical and aromatic plants that have been used since mankind have become, a
focus of interest especially due to increased health concerns and safe food demand in recent years. It
can be demonstrated that the pharmaceutical industry takes a large share in R & D expenditures made
as a result of the increase in the importance of pharmaceuticals and aromatic plants. Data from the
World Health Organization show that 70-80% of the world's population benefits from traditional
medicine. In this direction, approximately 20,000 in the world; in our country, there are plants used for
about 500 medical purposes. Medicinal and aromatic plants in traded is used in 50% food, 25%
cosmetics and 25% pharmaceutical industry. The rose takes part the fragrant plant parts used in the
field of medicinal and aromatic plants. It has an important role in food, perfumery and cosmetic
industry. Besides this usage roses are curtained some benefits such as sedative, anti-stress property,
hemostatic, stomach, liver, intestines, fever and skin disease therapeutic and anti-inflammatory
functions. General usage areas of rose in our country; used as raw material in the production of
products such as dried rose, rose oil, rose syrup, rose jam, rose water and the use of flavouring and
colouring agents in the formulation of delight products etc. For this purpose, tea made by using 3
different dried bud roses and 3 different dried rose leaf purchased in Isparta and zmir market were
investigated in terms of antioxidant properties. The roses were kept in boiled water at 98 C for 5
minutes and filtered at the end of the time. Total phenolic substance by Folin-Ciocalteu method,
antioxidant capacity by TEAC method and total flavonoid determination were done in the samples
which arrived at room temperature. When the results of the analyses are examined, the total amount of
phenolic material 5,241-166,355 mg/200 mL tea, the total amount of flavonoids 2,019-14,825 mg/200
mL tea and the antioxidant capacity values 0.64-10.78 M troloks/200 mL tea are found. In all
analyzes, dry bud results were found to be lower than dry leaves. In addition, there was a statistically
significant difference between the varieties (p0.05). Besides its pleasant smell and comfortable drink,
it also has antioxidant properties that rose tea can be an alternative to other herbal teas, it is thought
that the consumption can be widespread and the usage areas can be expanded with the works to be
done.

KEYWORDS
Antioxidant capacity, tea, phenolic substance, rose,

Corresponding Author: ZLEM ZLEM AINDI, ozlemcagindi@gmail.com

1
CELAL BAYAR NVERSTES

1224
 Poster Session 9
Submission ID: 1167

THE CYTOTOXIC AND APOPTOTIC EFFECTS OF THE YOUNG

SHOOTS EXTRACT OF TAMMUS COMMUNIS ON
HEPATOCELLULAR CARCINOMA

1 1 1 2 1
NAL EGELI , BESTE YURDACAN , HUZEYFE HURIYET , GAMZE GNEY ESKILER , IIL EZGI ERYILMAZ , HULUSI
1 1 1 1
MALYER , GLAH EENER , TOLGA AVA , BERRIN TUNCA

ABSTRACT
Plants are an important sources as development of novel therapeutic agents in cancer
treatments because most chemotherapeutic drugs have been improved or isolated from plants or their
synthetic derivatives. Tamus communis L. (Dioscoreaceae, named Sarmak locally), a perennial
herbaceous climbing plant, have some pharmacological effects including antioxidant, antiviral, anti-
inflammatory and antimicrobial. However, the anticancer effect of T.Communis extract has not known
well yet in the literature. In the current study, we aimed to determine the cytotoxic and apoptotic
effects of young shoots extract of T.communis on hepatocellular carcinoma. The cytotoxic effect of
T.communis extract on SNU-449, Hep G2 cells treated with different concentrations (50-250 g /ml)
was determined by WST-1 assay for 24,48 and 72h. The DNA damage and repair capacity in these
cells treated with the most effecttive concentration of T.communis young shoots extract were analyzed
by comet assay. Additionally, the morphology of apoptotic cells was examined by acridine
orange/ethidium bromide staining to support comet assay. The young shoots extracts from
T.communis displayed anti-proliferative activity on SNU-449 and Hep G2 cell lines (p<0.05). The cell
viability of Hep G2 and SNU-449 cells decreased to 58.6% and 80.0% at 250 g/ml of concentration
for 72 h, respectively. Hep G2 cells exhibited a higher sensitivity to the young shoots extracts than
SNU-449 cells due to associated with a more aggressive tumor phenotype. The young shoots extract of
T.communis exhibited less toxicity (12.7%) on HUVEC control cells. Additionally, the young shoots
extract of T.communis induced DNA strand breaks and apoptosis in SNU-449 and Hep G2 cells. The
cytoplasmic and nuclear shrinkage and chromatin condensation were observed on particularly Hep G2
cells. Consequently, young shoot extract of T.communis has displayed an anticancer properties due to
its pharmacological profile. However, this study may be improved by extraction of antitumoral
activity different plant parts (rhizome and stem) and determination in amount of phenanthrene or
phenolic compounds

KEYWORDS
Hepatocellular carcinoma, Tammus communis, Cytotoxicity, Apoptosis, Extraction

Corresponding Author: BESTE YURDACAN, besteyurdacan93@gmail.com

1
ULUDA NVERSTES
2
SAKARYA NVERSTES

1225
 Poster Session 9
Submission ID: 1168

A STUDY OF ANTIMICROBIAL ACTIVITY OF CONSOLIDA

ORIENTALIS AND SPARTIUM JUNCEUM

1 ! 1 1
ERDOGAN GUNES , AHMET UYSAL , YUSUF DURAK , GOKHAN ZENGIN

ABSTRACT
For a long period of time, plants have been a valuable source of natural products for
maintaining human health, especially in the last decade, with more intensive studies for natural
therapies. The World Health Organization estimates that plant extracts or their active constituents are
used as folk medicine in traditional therapies of 80% of the world's population. Therefore, such plants
should be investigated to better understand their properties, safety and efficiency. The aim of this
study are to determine the antimicrobial activities of methanol and water extracts from Consolida
orientalis and Spartium junceum plants distributing in Turkey. Antimicrobial activity of the tested
plant extracts were studied by the broth microdilution method. It was determined that Consolida
methanol extracts showed antibacterial activity at doses ranging between of 6.25-3.125 mg/ml against
S. aureus, S. enteritidis and E. faecalis standart bacteria. It was found that methanol extract of
Consolida had antifungal activity at a dose of 6.75 mg/ml against C. albicans and C. parapsilosis.
However water extracts of Consolida had an antibacterial effect only against E. faecalis at a dose of
6.25 mg / ml. When the methanol extracts of Spartium were evaluated it was seen that methanol
extracts were effective at a dose of 6.75 mg/ml on the S. lutea, E. faecalis and C. albicans. However
water extracts of Spartium showed no antimicrobial activity. It was concluded that methanol and water
extracts of Consolida orientalis and Spartium junceum have weak antimicrobial activity.

KEYWORDS
Antimicrobial activity, Consolida orientalis, Spartium junceum, Turkey

Corresponding Author: ERDOGAN GUNES, erdogangunes@selcuk.edu.tr

1
SELCUK UNVERSTY

1226
 Poster Session 9
Submission ID: 1171

THE ANTIMICROBIAL ACTIVITY OF ONOSMA ISAURICUM

1 1
ERDOGAN GUNES , AHMET UYSAL

ABSTRACT
In many developing countries antibiotic resistance, adverse drug reactions, and the high costs
of antimicrobials have made management of infectious diseases ineffective. Natural products of higher
plants may be a source of new antimicrobial agents with possibly novel mechanisms of action. In this
study, it is aimed to investigate the antimicrobial activity of the extracts of methanol and water of
Onosma isauricum on some microorganisms. The antimicrobial activity was evaluated according to
the broth microdilution method by using Escherichia coli, Pseudomonas aeruginosa, Staphylococcus
aureus (MSSA), Klebsiella pneumoniae, Staphylococcus aureus (MRSA), Salmonella enteritidis,
Sarcina lutea, Eenterococcus faecalis, Candida albicans and Candida parapsilosis. It was found that
methanol extracts of Onosma had antimicrobial activity at a dose of 6.25 mg/ml against S. lutea, E.
faecalis, C. albicans and C. parapsilosis. When the water extracts of Onosma isauricum were evaluated
it was seen that water extracts were effective at a dose of 3.125 mg/ml on the S. aureus (MSSA) and S.
aureus (MRSA). As a result, we concluded that methanol and water extracs of Onosma isauricum have
weak antimicrobial effect.

KEYWORDS
Antimicrobial activity, broth microdilution, Onosma isauricum

Corresponding Author: ERDOGAN GUNES, erdogangunes@selcuk.edu.tr

1
SELCUK UNVERSTY

1227
 Poster Session 9
Submission ID: 1172

INVESTIGATION OF EFFECTS ON RIPENING OF DIFFERENT

TYPES OF SPICES USED IN WHITE CHEESE PRODUCTION

1 2
ZEKAI TARAKI , FAHRETTIN DEVEC

ABSTRACT
INVESTIGATION OF EFFECTS ON RIPENING OF DIFFERENT TYPES OF SPICES
USED IN WHITE CHEESE PRODUCTION* Zekai TARAKI1, Fahrettin DEVEC2 1Department
of Food Engineering, Agricultural Faculty, Ordu University, Ordu, Turkey 2Ordu Food Agriculture
and Livestock Directorate, Ordu, Turkey *This research was supported with TF-1423 project number
by ODU-BAP. 1zetarakci@hotmail.com In this study, six types of white cheese, one of them is control
sample, were produced. While control cheese was produced without adding any spices; the others
were processed to cheese by adding black cumin, dried mint, thyme rubbed, red pepper flakes and isot
pepper as 3% according to the milk used. Then, all cheeses were vacuum-packed and ripened at 71C
during 3 months and dry matter, fat, pH, titratable acidity, salt, total protein, ripening rate, nonprotein
nitrogen rate (NPN), amino nitrogen rate, electrophoretic casein fractions, textural properties and
sensory analyses were performed by taking cheese samples during the 2nd, 15th, 30th, 60th and 90th
days of ripening. The results were compared in terms of the types of cheese and ripening period,
statistically. The values of adhesiveness, springiness and resilience parameters of texture profile
analyses and also the values of structure-texture parameters of sensory analyses were not statistically
significant (P>0.05). The values of fat and the values of springiness parameters of texture profile
analyses were determined statistically significant (P<0.05). All of the other parameters were obtained
statistically significant (P<0.01). As a result of sensory evaluation made by panelists, ripened cheeses
had more acceptability than fresh cheeses. Also as cheese type, samples with black cumin, mint and
thyme exceled in common with control sample. Keywords: Spice, white cheese, ripening.

KEYWORDS
Spice, white cheese, ripening

Corresponding Author: ZEKA TARAKI, zetarakci@hotmail.com

1
ORDU NVERSTES
2
ORDU FOOD AGRCULTURE AND LVESTOCK DRECTORATE

1228
 Poster Session 9
Submission ID: 1174

USE OF SOME NATIVE TREES WITH MEDICINAL

QUALIFICATIONS IN URBAN GREEN AREAS: ANTAKYA (HATAY-
TURKEY)

1 2 1
ELIF BOZDOAN SERT , ZGR KAHRAMAN , SEMA GLER

ABSTRACT
Trees have been important for human beings in terms of benefits as shelter, obtaining food and
clothing materials, etc. from past to present. For this reason, some of the species have produced,
growth and even its protection have provided. With the establishment of cities as living space, planting
of green areas such as parks, streets, cemeteries and gardens have carried out within certain
frameworks. At this time, initially plants with food and medicinal qualification have preferred to bring
to the cities. By this way, use of many of the species with both aesthetic and functional qualities in
planting urban green areas have leaded. Trees that are the main material of the plantation are
indispensible elements of urban landscape with many functional features such as shadowing,
orientation, focusing, and accumulation of pollutants. Especially, because of its easier adaptation to
environment, native species have used often in urban green areas. With this study, it was aimed to
determine native tree species with medicinal qualification used in the green areas of Antakya (Hatay).
The study was conducted in parks (Atatrk Park, Vali rgen Park), public institutions gardens
(Governorship, General Directorate of State Hydraulic Works, Provincial Directorate of Health), urban
roads, and Mustafa Kemal University Tayfur Skmen Campus in Antakya city center between April-
May 2016. As a result, it was determined that native twenty tree taxa with medicinal qualification
were used in urban green areas of Antakya. In this context, all taxa were studied in terms of aesthetic
(color, texture, form) and functional (shadow, focus, orientation) qualifications important in landscape
architecture and other qualifications such as medicinal.

KEYWORDS
Native trees, urban green area, landscape, medicinal, Antakya

Corresponding Author: ELF BOZDOAN SERT, ebozdogan@mku.edu.tr

1
MUSTAFA KEMAL UNVERSTY, FACULTY OF ARCHTECTURE DEPT. OF LANDSCAPE ARCHTECTURE
2
ANAKKALE ONSEKZ MART UNVERSTY, FACULTY OF ARCHTECTURE AND DESGN, DEPT. OF
LANDSCAPE ARCHTECTURE

1229
 Poster Session 9
Submission ID: 1176

BIOLOGICAL ACTIVITIES OF SALVIA PACHYSTACHYS FROM

ANATOLIA TURKEY

1 2 3 4 5
ELIF VARHAN ORAL , HAVA BATIBAY , ABDULSELAM ERTA , MEHMET FIRAT , HILAL SARUHAN FIDAN ,
5 3 2 6
HSEYIN ALKAN , ESRA YARI , BERRIN ZIYADANOULLARI , GLATI TOPU

ABSTRACT
Since ancient times, Salvia species have been used in folk medicine for the treatment of
diabetes and skin diseases such as psoriasis and eczema. These species have been the subject of
extensive pharmacognosic researches that were intended to identify biologically active compounds.
Particular attention has been shown to the members of the genus Salvia due to their wide range of
important biological activities such as antifungal activity, antitumor activity, antibacterial activity,
antiviral activity, cytotoxic activity, antioxidant activity, treatment of heart disease, and
antimycobacterial activity . The aim of this study was to determine the phenolic and flavonoid contens
(determined as pyrocatechol and quercetin equivalents, respectively), antioxidant (DPPH free radical
scavenging activity, -carotene bleaching assay, CUPPRAC, ABTS cation radical scavenging activity)
and anticholinesterase (acetyl- and butrylcholinesterase enzymes) activities of these salvia species
were tested. It was determined that working in all extracts not show acetylcholinesteras activity but
show a moderate butyrylcholinesterase activity. Furthermore the ethanol extracts of all parts of (stems,
leaves, flowers, roots and mixed parts) specie showed high antioxidant activity in all antioxidant tests.
Especially the flower of Salvia Pachystachys showed high antioxidant activity in ABTS assay.

KEYWORDS
Salvia pachystachys, Total Phenolic-Flavonoid Content, Antioxidant, Anticholinesterase

Corresponding Author: ELF VARHAN ORAL, evarhan@dicle.edu.tr

1
DCLE UNVERSTY, FACULTY OF PHARMACY, DEPARTMENT OF ANALYTCAL CHEMSTRY, DYARBAKIR
2
DCLE UNVERSTY, FACULTY OF SCENCE, DEPARTMENT OF CHEMSTRY, DYARBAKIR
3
DCLE UNVERSTY, FACULTY OF PHARMACY, DEPARTMENT OF PHARMACOGNOSY, DYARBAKIR
4
YZNC YIL UNVERSTY, FACULTY OF EDUCATON, DEPARTMENT OF BOLOGY, VAN
5
DCLE UNVERSTY, FACULTY OF PHARMACY, DEPARTMENT OF BOCHEMSTRY, DYARBAKIR
6
BEZMALEM VAKF UNVERSTY, DEPARTMENT OF PHARMACOGNOSY AND PHYTOCHEMSTRY,
STANBUL

1230
 Poster Session 9
Submission ID: 1178

DETERMINATION OF PHENOLIC AND MINERAL COMPOUND

COMPOSITION IN SOME FRUIT JUICE MARKETED IN NGDE

1 2
EZGI KARAKA , RIFAT BATTALOLU

D
ABSTRACT
In this research, it is aimed to determine total phenolic and mineral contents of some kind of
fruit juices (apricot, sour cherry, orange, peach and mixed) which was bought from markets in Nide

TE
and to evaluate with the standards. Firstly, pH, total acidity and hydroxymethyl furfural (HMF)
analysis of the collected samples were achieved. Then, total phenolic contents and some mineral and
metal contents (potassium, iron, zinc, aluminium and copper) were determined and evaluated with
standards of regulations. The average pH value of the samples was found as 3.780.20. The average of
EN
HMF concentrations of the samples was found as 4.993.04 mg/L. Average of total phenolic material
contents for all samples was found as 153.0342.93 mg/L. According to analysis of mineral and metal
contents results, potassium, iron, zinc, aluminium and copper contents were between 219.88-425.98
mg/L, 0.025-0.056 mg/L 0.588-1.965 mg/L, 0.004-0.014 mg/L, 0.187-0.557 mg/L for sour cherry
ES
juices,; 320.58-580.88 mg/L, 0.097-3.653 mg/L, 0.385-2.086 mg/L, 0.002-0.134 mg/L, 0.115-1.129
mg/L for apricot juices, 205.88 and 489.88 mg/L, 0.049 and 0.115 mg/L, 0.777mg/L, 0.028 and 0.083
mg/L, 0.076-0.233 mg/L for orange juices, 230.08 and 371.78 mg/L, 0.128 and 0.137 mg/L, 0.389 and
0.734 mg/L, 0.066 and 0.110 mg/L, 0.114 and 0.258 mg/L for peach juices and 165.38-417.38 mg/L,
0.027-0.1112 mg/L, 0.359-1.087 mg/L, 0.025-0.073 mg/L, 0.049-0.561 mg/L for mixed fruit juices
PR

respectively.

KEYWORDS
Fruit juice, total phenolics, hydroxymethyl furfural, potassium, zinc, ferrum, copper,
T

aliminium.
O
N

Corresponding Author: RFAT BATTALOLU, rbattaloglu@ohu.edu.tr

1
2
MER HALSDEMR NVERSTES

1231
 Poster Session 9
Submission ID: 1179

GC-MS ANALYSIS OF ESSENTIAL OIL AND FATTY ACID

COMPONENTS OF CENTAUREA PAPHLAGONICA PLANT

1 1 1 1
ALI RIZA TFEK , BRAHIM DEMRTA , EBRU DERELL TFEK , MUHAMMED ALTUN

ABSTRACT
Centaurea plant species that have medicinal properties are among the largest species of the
asteraceae family. Because of these properties, it is used by human for therapeutic purposes. There are
about 600 centaurea species in the world. There are about 187 species in Turkey. 120 of these species
are endemic species. In this study, volatile oil in the plant was obtained by hydrodestination method.
Fatty acid of plant were obtained according to the method of mesagenization in the hexane organic
solvent. Component analyzes in volatile and fatty acid were determined by GC-MS instrument.
According to the results obtained, spathulenol and gamma-elemene compounds in volatile oil were
identified as the main components. In fatty acid, hexadecanoic acid was identified as the main
component.

KEYWORDS
Asteracea, centeurea paphlagonica, essential oil, fatty acid

Corresponding Author: AL RIZA TFEK, alirizatufekci@gmail.com

1
ANKIRI KARATEKN NVERSTES

1232
 Poster Session 9
Submission ID: 1182

IN VITRO NEMATOSTATIC ACTIVITY OF ALLIUM TUBEROSUM

(ROTTL.) ON STEM AND BULB NEMATODE (DITYLENCHUS
DIPSACI)

1 1
ELIF YAVUZASLANOLU , ALI BILGI

ABSTRACT
Allium tuberosum (Rottl.) (Alliaceae) is a native growing plant in Southern Asia named
commonly as garlic chive. It produces small bulb and fast growing leaves. The leaves are cultured and
used for treating abdominal pain, diarrhea, hematemesis, snakebite and asthma in China. The whole
plant has also antibacterial, cardiac, depurative, digestive, stimulant, stomachic and tonic activity. The
stem and bulb nematode (Ditylenchus dipsaci) is one of the important constraints of a wide range of
plant species with main host of onion and garlic. It reproduces endoparasitically and damages plants.
Nematostatic activity of garlic chive on D. dipsaci was tested in vitro. Leaves of garlic chive were
dried at room temperature for two to three weeks. A one gram of dried leaves grinded and extracted in
10 ml methanol for 48 hours at room temperature on a magnetic stirrer. Dried methanol extract diluted
with water to 3% concentration. Nematodes were treated with 0,5 ml, 1 ml and 1,5 ml of aqueous
solution of garlic chive methonol extract. Negative control contained sterilized tap water. Each
treatment contained averagely 50 nematodes. Total volume in each treatment was completed to 5 ml
using sterilized tap water in 9 cm plastic petri dishes. Nematodes incubated at 15 C for 48 hours.
Moving and motionless nematodes were counted under microscope after 48 hours of exposure in the
treatments. Motionless nematode rate increased significantly at garlic chive treatments. While
motionless nematode rate was 6% in negative control treatment, it was recorded 35, 86 and 78% in
0,5, 1 and 1,5 ml garlic chive treatments, respectively. Preliminary results indicate that the methanol
extract of garlic chive shows nematostatic effect on D. dipsaci at 48 hours exposure in vitro. The
effective content of methanol extract of garlic chive and effect of active content under in vivo
conditions are next step of the study.

KEYWORDS
garlic chive, nematostatic activity, stem and bulb nematode, Allium tuberosum, Ditylenchus
dipsaci

Corresponding Author: ELF YAVUZASLANOLU, eyavuzaslanoglu@kmu.edu.tr

1
KARAMANOLU MEHMETBEY NVERSTES

1233
 Poster Session 9
Submission ID: 1183

AFYONKARAHISAR MEDICAL AND AROMATIC PLANTS

CENTRAL OFFICE INTRODACTION AND ACTIVITIES

1 2
EMEL YILDIZ , CAFER LKAY YILDIRIM

ABSTRACT
The centre was established in Afyonkarahisar in 2013 of Ministry Of Forestry And Water
Management. The purpase of establistment inccreases identifying the medical and aromatic plants
which are grown in our country and uses the national wealth potential the most productive. The
Medical And Aromatic Plants Centre was establisted in 45000m aera. The centre has got 1200m in
door area and 5000m growing area of plants.There are demonstration greenhouse, conference and
assembly hall, laboratory, sales and working offices, production greenhouse in indoor area. From the
opening day to this day there are a lot of visitors who are from different places of our country and
interested in medical and aromatic plants. The visitors are acquainted with and making introduction of
these plants. The scientific Project studies are developed with and applied to Afyon Kocatepe
Universty. The growing conditions of the plants which are medical and aromatic and grown in our
nature are produced in our introduction area.

KEYWORDS
Afyonkarahisar, Aromatic plants, Medical plants, Ministry Of Forestry And Water
Management, Plant growing

Corresponding Author: EMEL YILDIZ, emelyildiz@ogm.gov.tr

1
ORMAN VE SU LER BAKANLII, ORMAN GENEL MDRL, ESKEHR ORMAN BLGE
MDRL. AFYONKARAHSAR TIBB VE AROMATK BTKLER MERKEZ MDRL
2
ORMAN VE SU LER BAKANLII, ORMAN GENEL MDRL, ESKEHR ORMAN BLGE
MDRL

1234
 Poster Session 9
Submission ID: 1184

ANTIMICROBIAL AND ANTIFUNGAL ACTIVITY OF FABRICS

DYED WITH VIBURNUM OPULUS AND ONION SKINS

1 2 2 2 2
HANDAN APCI , FAZLIHAN YILMAZ , CEM VURAL , BRAHIM BAHTIYARI , HSEYIN BENLI

ABSTRACT
Microorganisms such as bacteria and fungi that we can encounter almost everywhere in our
daily life, and varieties that are pathogenic against special diseases can cause serious health and
hygiene problems. The negative effects of hygienic artificial products on natural environment and
ecological balance are also quite high. For this reason, place of various products with antimicrobial
activity gains importance from day to day. As is known, textile products can provide a suitable
environment for the development of microorganisms. In this context, we aimed to develop textile
products which can provide antimicrobial and antifungal effect. For this purpose, woolen fabrics were
dyed with onion (Allium cepa) skins and juice of Viburnum opulus plant fruit in our study. The
dyeings were carried out without using of any mordanting agent and no prior extraction of the herbal
sources were carried out in other words these natural dye sources (onion skins and juice of Viburnum
opulus plant fruit) were directly added to the dye bath. Samples after dyeing with these natural dye
sources were tested in terms of antimicrobial activity using two bacteria (Escherichia coli,
Enterobacter aeroginosa) and a yeast strain (Candida albicans). As a result, it was observed that onion
skin and Viburnum fruit juice had an antimicrobial effect. While the dyed fabric with Viburnum juice
showed higher activity on bacterial strains, the onion skin had a higher effect on the yeast. Viburnum
provided the highest activity on E. coli, whereas onion skins showed the highest activity on C.
albicans.

KEYWORDS
Antimicrobial textiles, bacteria, biotechnology, fungi, natural dye

Corresponding Author: HANDAN APCI, hsapci@cu.edu.tr

1
UKUROVA NVERSTES
2
ERCYES NVERSTES

1235
 Poster Session 9
Submission ID: 1185

EVALUATION OF WHEAT GERM OIL EFFECTS ON ERECTILE

DYSFUNCTION INDUCED BY RESTRAINT STRESS IN RATS.

1 2 3 4
DR. EMRE ZTOPRAK , DO. DR. MEHMET MESUT PKN , PROF. DR. AYE SADE AHN , ECZ. MEVRA AL ,
3
DO. DR. BURAK CEM SONER

ABSTRACT
Policosanols in wheat germ oil structure is used for increasing physical and sexual
performance. Wheat germ oil has antioxidant properties because of its natural vitamin E ingredient. In
this study we aimed to evaluate the effects of wheat germ oil; which is a traditional herbal medicinal
product used for erectile dysfunction; on experimental erectile dysfunction model in rats. For this
purpose; male Wistar Albino rats were subjected to restraint stress one hour per day for 10 days in a
special tube with holes for air supply to perform erectile dysfunction model. Rats for divided to 2
groups. In control group (n=5), rats were treated with 3 ml/kg/day tap water for 10 days during
restraint stress period by oral gavage. In wheat germ oil group (n=5), rats were treated with 3
ml/kg/day wheat germ oil for 10 days during restraint stress period by oral gavage. At 11th day; i.m.
ketamine (80 mg/kg) and i.m. xylazine (8 mg/kg) were administered for anesthesia. Under anesthesia,
intra cavernous pressure (ICP) and mean arterial pressure (MAP) from right carotid artery was
measured after canulation. All data of systemic arterial and cavernosal pressure were recorded by
MP36 computerized system. After 20 minutes of resting time; cavernous nerve was stimulated for 1
minutes by electrical stimulation (5 V, 20 Hz, 1 milisecond). During and after the stimulation ICP,
MAP, ICP/MAP rates and corpus cavernosum detumescence time was evaluated. Mean ICP, MAP,
ICP/MAP and corpus cavernosum detumescence time measured in placebo group were 35,55,01
mmHg, 97,612,73 mmHg, 0,360,05 and 133,416,03 seconds respectively. In wheat germ oil group
these values were 45,17,6 mmHg, 106,28,23 mmHg, 0,510,06 ve 256,227,53 respectively.
Statistical analyses between groups were evaluated by t-test. Our results showed that statistically
significant difference between the groups was only found in detumescence time (p=0.01). Our
limitation of this preliminary study is not having a group to evaluate normal ICP, MAP and
detumescence values without stress but; according to the statistical significance in detumescence times
between two groups; we can speculate that wheat germ oil may be a beneficial herbal medicinal
product as a complementary medicine to improve erectile function.

KEYWORDS
wheat germ oil, erectile dysfunction

Corresponding Author: DO. DR. BURAK CEM SONER, burakcemsoner@gmail.com

1
HELVACIZADE GIDA LA KMYA SAN. TC. A.
2
KONYA NECMETTN ERBAKAN NVERSTES MERAM TIP FAKLTES ROLOJ ABD
3
KONYA NECMETTN ERBAKAN NVERSTES MERAM TIP FAKLTES TIBB FARMAKOLOJ ABD
4
KTO KARATAY NVERSTES TIP FAKLTES TIBB FARMAKOLOJ ABD

1236
 Poster Session 9
Submission ID: 1186

DETERMINATION OF DEOXYNOJIRIMYCIN BY DEVELOPED AND

VALIDATED AN HPLC-FLD METHOD IN LEAVES OF MULBERRY
VARIETIES FROM TURKEY

1 1
NURANIYE ERUYGUR , EMRAH DURAL

ABSTRACT
1-deoxynojirimycin (DNJ), a piperidine alkaloid, is known to be one of the most potent a-
glycosidase inhibitors. In previous studies, more than twenty polyhydroxylated alkaloids have been
identified in leaves of Morus species. The leaves of M. alba and M. nigra used in diabetes mellitus
patients nutraceutical foods due to their antihyperglycemic activity in Korea and Japan. Therefore, it
is necessasary to analysis of 1-deoxynojirimycin contents in pharmaceutical preparations and herbal
drugs made form Morus leaves in order to quality control. Hence, a rapid, sensitive and reliable high
performance liquid chromatographic method for the determination of 1-deoxynojirimycin (DNJ) in
Morus alba L. and Morus nigra leaves with fluorimetric detection after precolumn derivatization with
9-fluorenylmethyl chlorformate (FMOC-Cl) was developed. DNJ in Morus alba L. and Morus nigra L.
leaves was extracted with 0.05 mol/L HCI, derivatized with FMOC-Cl, and analyzed by high
performance liquid chromatography equipped with fluorescence detector. The separation was
performed on GL Sciences Inertsil ODS-3 C18 column (4.6 x 250 mm, 5 m), mobile phase consisted
of acetonitrile - 0.1 % aqueous acetic acid (50:50, v/v) with a flow rate of 1.0 mL/min at 26C. The
calibration curve was linear in the range of 0.1- 30 mg/L, the correlation coefficient (r2) was 0.9985.
The limit of detection (3s/b) and quantification (n=10) were 1.07 and 3,27 ng mL-1, respectively.
Intraday and interday method precision (n=5) were between 7.26; 3.98 and 7.03; 3.52 (RSD%),
respectively. Intraday and interday method accuracy (n=5) were between (-6.68) - 2.80 and (-8.35) -
3.77 (RE%), respectively. The method recovery (n=3) was between 97,16% - 107,85%. The obtained
robustness values from emission and excitation detection, mobile phase ingredients and flow rates
changes were show that method was very strong. The results showed that the content of DNJ in leaves
of Morus alba L. were between 0,9% - 1,1% and in leaves of Morus nigra L. was 1,0%.

KEYWORDS
HPLC, FLD, Morus alba, Morus nigra, Deoxynojirimycine, Validation

Corresponding Author: NURANYE ERUYGUR, neruygur@cumhuriyet.edu.tr

1
CUMHURYET NVERSTES ECZACILIK FAKLTES

1237
 Poster Session 9
Submission ID: 1187

ANTIMICROBIAL AND ANTIOXIDANT ACTIVITY OF ECHINOPS

EMILIAE (ASTERACEAE)

1 2
HANDAN APCI , CEM VURAL

ABSTRACT
The aim of this study was to investigate the antimicrobial and antioxidant activities of
different extracts from Echinops emiliae Schwarz ex P. H. Davis. Antimicrobial activity was estimated
against several common human pathogenic bacterial strains using the agar disc diffusion and minimal
inhibitory concentration assays. Antioxidant activity was evaluated using the DPPH radical-
scavenging assay and total phenolic content methods. On the light of these experiments, E. emiliae
would seem to be an important natural antioxidant. Antimicrobial and antioxidant of E. emiliae have
not been reported up to now. The results of this study obviously reported that the antimicrobial and
antifungal activity could be change with used extracts. Also, the micro dilution method was more
sensitive than disk diffusion. This study is first report on the biological activity of E. emiliae as
regarded endemic species from Turkey. The results show that E. emiliae could use in the treatment of
some illness.

KEYWORDS
Anti-bacterial; antifungal; Composite; Echinops; phenolic content; plant extracts

Corresponding Author: HANDAN APCI, hsapci@cu.edu.tr

1
UKUROVA NVERSTES
2
ERCYES NVERSTES

1238
 Poster Session 9
Submission ID: 1188

FERULIC ACID AS A FUNCTIONAL FOOD COMPOUND AND ITS

IMPORTANCE

1 2
EYDA BOSTANCI , LKAY KOCA

ABSTRACT
Ferulic acid (4-hydroxy-3-methoxycinnamic acid) is one of the most abundant phenolic acid
in plant kingdom. It occurs from metabolism of phenylalanine and tyrosine by Shikimate pathways in
plants. It is present at high concentrations in many products, including vegetables, fruits, cereals, and
coffee. It is mainly conjugated with mono- and oligosaccharides, polysaccharides, lipids, and
polyamines, and seldom found in a free forms in plants. It can be absorbed along the entire
gastrointestinal tract and easily metabolized by the liver. It has many therapeutic properties like
antioxidant, anti-inflammatory, antimicrobial, anti-thrombosis, and anti-cancer activities. It protects
against many disorders such as Alzheimers disease, cardiovascular disease, diabetes mellitus, and
colon cancer. Also, ferulic acid is used in the food industry. The raw material of the production of
vanillin is ferulic acid. In this review, the chemistry, natural resources and human health effects of
ferulic acid are discussed.

KEYWORDS
Functional food, secondary metabolite, phenolic acids, ferulic acid

Corresponding Author: EYDA BOSTANCI, drseydabostanci@gmail.com

1
STANBUL KLTR NVERSTES
2
SAMSUN 19 MAYIS NVERSTES

1239
 Poster Session 9
Submission ID: 1189

MILK THISTLE (SILYBUM MARIANUM), POSSIBLE CLINICAL

EFFECTS AND SAFETY

1 1 1
DUYGU AAGNDZ , MAKBULE GEZMEN KARADA , HILAL YILDIRAN

ABSTRACT
Milk thistle(Silybum marianum) is being used for the treatment of liver and gall bladder
illnesses and for the protection of liver against toxins. Today, researches have focused on its effects as
cytoprotective, antioxidant and anticarcinogenic effects as well as on its protective effects against
death cup(Amanita phalloides). Its active substance is silymarin, which is mostly found in the seeds.
Silymarin is a flavonoid complex, which also includes silybin, silychristin and silydianin.Milk thistle
seeds include 1.5-3% flavonolignan, also known as silymarin, 20-30% non-volatile oil (60% linoleic
acid, 30% oleic acid, approximately 9% palmitic acid), 25-30% protein, 0.038% tocopherol and 0.60%
sterol.There are frequently 70-80% flavonolignan(silybin, silychristin and silydianin), which are also
known as silymarin, in milk thistle. Silymarin enters into enterohepatic circulation in the body so its
concentration in the liver is generally higher than serum. Absorption of silymarin in gastrointestinal
system is rather low(%20-50).For that reason, production of extract with high concentrations of active
substance has become popular. The clinical researches suggest that milk thistle extract has positive
effects on Hepatitis A, alcoholic cirrhosis and liver illnesses caused by exposure to harmful chemical.
It has been found out that, as as anti-fibrotic agent, it has reduced the collagen accumulation in liver
caused by serum procollagen Type III-formation.In a number of other researches it has been stated that
silymarin has shown antienflamatuar effect and has regulated inflamatuar mediators such as; tumor
necrosis factor(TNF)-, nitrous oxide, interleukin-6 and interleukin-1 receptor antagonist.Within this
frame, it is considered that it has a role in the treatment of infectious diseases.In some studies, it has
been found out that milk thistle has reduced free radical production and hepatotoxicity lipid
peroxydation. Studies on its anticarcinogenic effects are still going on and there are studies suggesting
that it is especially effective in the treatment of prostate cancer related with sex hormones.However,
American Family Physicians Association(AAFP) states that the evidence regarding its effectiveness in
its use in the clinic is poor. As for the assessments regarding its side effects, it is stated that it is
generally well tolerated, but it has caused itchiness, eczema, skin eruption and anaphylaxis in some
cases. However, effective and safe dose in clinic use is contradictorUS Food and Drug
Administration(FDA) stated in 2001 that it is safe to be added in malt based drinks to support bitter
aroma. However, evidence for its safety in clinical use is inadequate. Although it is not mentioned
very significant interference in general in terms of drug interactions, in some studies it has been stated
that it may form herb-drug interference by interfering with cytochrome P(CYP) microsomal enzyme.
In some in vitro studies, it has been found out that it has significantly induced CYP3A4 isoform and in
some other studies that it may inhibit the activities of enzymes by irreversibly binding to CYP3A4 and
CYP2C9 enzymes. It has been found out that it may inhibit CYP2C9 enzyme in liver and it has
reduced metabolite rate related with CYP2C as the result of use of Losartan with Milk Thistle. As a

Corresponding Author: DUYGU AAGNDZ, duyguturkozu@gazi.edu.tr

1
GAZ NVERSTES SALIK BLMLER FAKLTES BESLENME VE DYETETK BLM

1240
result, although it has been stated in some studies that milk thistle has significant effects in protection
from and treatment of some diseases in clinic, evidence for that is poor. Further studies are required on
this subject. Attention should be paid for its safety and for its possible drug interactions and it should
not be used without an advice from a physician. Selected References Gezmen-Karada M, Trkz D,
Topaa Kapucu D.Gztepe Tp Dergisi 2013; 28(4):164-70. Rainone F. Milk thistle Am Fam
Physician 2005;72(7):1285-8. Ross SM. Milk Thistle(Silybum marianum):An Ancient Botanical
Medicine for Modern Times.Holist Nurs Pract 2008;22(5):299300.

KEYWORDS
Milk Thistle (Silybum Marianum), Clinical Effects, Safety

1241
 Poster Session 9
Submission ID: 1192

SALVIA SPECIES AND DRUG INTERACTIONS

1 2 2 2
MEVRA AL , AYE SAIDE AHIN , ESRA BYK , BURAK CEM SONER

ABSTRACT
Salvia belongs to the family of Lamiaceae (Labiatae), which has a cosmopolitan distribution
with 200 genera and 3000 to 4000 species. There are about 900 species of Salvia, one of the widest
generals of the family. The plants are herbaceous or bushy, perennial, rarely biannual or annual,
mostly aromatic, with flowers in different colors. Salvia L. is widely distributed in various parts of the
world such as the Mediterranean region, South Africa, Central and South America, and Southeast
Asia. The main center of species in Asia is Anatolia. There are 43 endemic species in Anatolia. In
addition to diversity of species, Salvia also has differences in bioactivity between species. There are
very different pharmacological effects between species. For thousands of years, it has been used in the
treatment of many diseases such as flu, bronchitis, tuberculosis, hemorrhage, oral cavity and throat
inflammation. The main component of Salvia oil is volatile monoterpenoids. It has been reported that
S. officinalis (Sage), S. lavandulifolia and S. fruticosa Miller species with high essential oil content
have antibacterial properties. Dan-shen (S. miltiorrhize root extract) is defined as a drug used in the
Chinese Pharmacopoeia to treat heart and circulatory system diseases, insomnia and acute arthritis
pain in patients with rheumatism. Salvianolic acid in Dan-Shen is a potent inhibitor of H+/K+ ATPase
and has been shown to be effective in reducing gastric acid secretion. However, the antiulcer effect is
less than the well-known antiulcer agent omeprazole. The antispasmodic effect of Salvia varies by
species. Sage and S. fruticosa Mil. has antispasmodic activity while S. verbenacea L. species was
observed to increase spasmodic efficacy. Sage leaves show antioxidant properties due to its phenolic
structure. Pharmacological studies show that S. lavandulifolia Vah., S. fruticosa Mil. and S. aegypyaca
species have strong hypoglycemic effects. The Mexican sage, S. divinorum, is well known for its
hallucinogenic effect. The components thujone and camphor are responsible for volatile oil toxicity. -
thujone inhibits gamma-aminobutyric acid-A receptor and oral use of camphor even in small
quantities may lead to serious adverse effects resulting in tonic-clonic seizures and death. Inhalation or
oral consumption of Sage and its commercial products may result in convulsions. Essential oil can
reduce the effects of central nervous system depressants such as diazepam and phenobarbital while
potentiating the effects of stimulants. An experimental neurotoxicity study in rats showed that the
subconvulsive limit for volatile oil is 0.3 g/kg dose. The anticholinesterase (AChE) activity observed
in the components of the Salvia species has been investigated clinically in the treatment of Alzheimer's
disease. Sage volatile oil (0.5 mg/ml) has been shown to inhibit AChE activity by 46%. Sage and S.
lavandulifolia volatile oil may interact with AChE inhibitor drugs used in the treatment of Alzheimer
due to AChE inhibitor effect. The Chinese herb 'Dan Shen' with antiplatelet activity has been reported
to cause bleeding by increasing the anticoagulant effect of warfarin. S. miltiorrhiza increases plasma
estrogen levels, ovarian and uterine prostaglandin-F2 levels, and this effect on the endocrine system
has been shown in immature rats. The estrogenic effect of Salvia may potentiate the effect of oral

Corresponding Author: BURAK CEM SONER, burakcemsoner@gmail.com

1
KTO KARATAY NVERSTES TIP FAKLTES TIBB FARMAKOLOJ AD.
2
NECMETTN ERBAKAN NVERSTES MERAM TIP FAKLTES TIBB FARMAKOLOJ AD.

1242
antidiabetics, folic acid antagonists and some corticosteroids and may induce hyperlipidemia and
inhibit the effect of hypolipidemic drugs. Due to the antidiabetic effect of thujone, sage should be used
with carefully antidiabetic drugs. Sage's aqueous extract is a specific inhibitor of CYP2C9, CYP2C19,
CYP2D6 and CYP3A4 enzymes so prolonged uses of sage may interact with the substrates of these
enzymes. Carnosol isolated from sage extract has been shown to reduce the minimum inhibitor
concentration of aminoglycoside in vancomycin-resistant enterococci. Carnosol and carnosic acid
show synergistic effect with gentamicin.

KEYWORDS
Sage, Salvia officinalis, Dan-shen, drug interaction

1243
 Poster Session 9
Submission ID: 1193

SCOPOLETIN: NATURAL SOURCES AND ITS EFFECTS ON HEALTH

1 1 1
LKAY KOCA , DAMLANUR KBRA AKIR , BELKIS TEKGLER

ABSTRACT
In recent years, coumarins, which are commonly used for preventing and treatment of many
diseases, are one of secondary metabolits produced by bacteria, fungus and some plants under abiotic
and biotic stress. The scopoletin ,involved in coumarins, is important health-related substance, has
been shown to exert biological activities such as antioxidant, anti-inflammatory, antihyperglisemic,
anticancer, hypouricemic, enhancer of melanin synthesis. Until today, the presence of scopoletin has
been found in many plants belong to Liliaceae, Asteraceae, Convolvulaceae, Loasaceae, Urticaceae,
Apiaceae, Fabaceae, Portulacaceae, Malvaceae families. In this rewiew, distribution of scopoletin in
plant kingdom, its biological and pharmacological properties and benefits on human health were
discussed.

KEYWORDS
Coumarin, scopoletin, health

Corresponding Author: DAMLANUR KBRA AKIR, cakirdamlanurkubra91@gmail.com

1
ONDOKUZ MAYIS NVERSTES, MHENDSLK FAKLTES, GIDA MHENDSL BLM, 55139,
SAMSUN

1244
 Poster Session 9
Submission ID: 1194

THE UNKNOWN HEALING 'TAURUS CREAM'

1 2
AYE NUR EN , PROF.DR. MUHITTIN DN

ABSTRACT
Turkey is like a continent about plants.According to the results of studies made until today,
its determined that Turkey has got so many plants as family, genus and species.Our countrys flora is
wealthier than its nighbours and other European countries.As its cultural treasure , besides this our
country has a comprehensive information treasure about ethnobotany.But with migrations to big cities
from villages and the development of technology; this knowledge is nearly disappearing.Medical and
aromatic plants has a wide marketing all over the World especially for medicine and food raw
material.Using of these plants from past until today and knowledge of their local names are so
important for humanity and transfering these to next generation.Turkey has got 10765 flowering plant.
In the world there are 800.000, in Turkey there are more than 9000 plants. The species which is
cultivated for food is about 3000. But the wild plants used for food are above 10000. World Health
Organisation determined nearly 20.000.Whereas the wild plants used for medical are at least 500. In
this study , in Mersin, Anamur citys, arklar village Havva SEZGN (60 year old) called womans
Taurus cream has been searched.She got it from her father sa Sal and its been a medicine for 21
years. She used in this cream these materials and plants; Juniperus oxycedrus L. subsp. oxycedrus,
Cyclamen cilicium Boiss.&Heldr., Abies cilicica (Ant.&Kotschy) Carr. subsp. Isaurica Coode &
Cullen, wax (bee houses), butter with no salt. She is determining the quantities according to the
capacity of case and her need for the sicks. For example, 2 kiloes of Juniperus oxycedrus L. subsp.
oxycedrus s Abies cilicicas resin gum are boiled with wax and butter after mashed.Then she is
adding the Cyclamen ciliciums tuber .After boiling these materials, she is lefting the mixture under
the sunlight.She puts this medicine on the injuried skin for 24 hours.If the injuried skin has turned to
white colour, it means that it had effected and healing is started. Havva Sezgins neighbours in the
village and people from Anamur city centre are visiting her and getting this Taurus cream for
healing. As the kind of illness; she is giving the cream, but not cancer people. Her father had said her
Dont refuse anybody and help everyone with this medicinal mixture. In this study; this cream was
also used for a woman erife K.(73 years old) who has got stained skin on face and burnt.The cream
was practised to her face and has got good results of it in 24 hours.This medical mixture is used for
these illnesses; skin injuries except cancer, foot&hand fungus ilness, face acnes, hair illness, knife
cuts, inflammatied wounds, bullet wound. In the conclusion of this ethnobotanical search, this
unknown medical cream can be brought in the science and by transfering next generations, positive
and useful results can be got.

KEYWORDS
Ethnobotany, flora, medicine , Taurus cream.

Corresponding Author: AYE NUR EN, anemon33sa@hotmail.com

1
SELUK NVERSTES
2
NECMETTN ERBAKAN NVERSTES

1245
 Poster Session 9
Submission ID: 1195

SENNA AND DRUG INTERACTIONS

1 2 1 1
AYE SAIDE AHIN , MEVRA AL , AYTEN DEMIRCI , BURAK CEM SONER

ABSTRACT
The senna fruit obtained from Cassia angustifolia is one of the most important herbal drugs for
the treatment of constipation worldwide. It is one of the most popular laxatives, especially in geriatric
patients. The major component of senna is anthraquinone glycosides. Senna leaf anthraquinones
include sennosides A, B, C and D, and palmidin A, rhein anthrone and aloe-emodin glycosides. The
fruit contains sennosides A and B and a closely related glycoside named sennoside A1. Senna is
usually standardized according to the amount of sennoside B content. At therapeutic doses, sennosides
provide relief from painful constipation by softening the consistency of the feces without disturbing
the routine defecation frequency. Senna increases colonic transit rate and colonic peristaltism. The
characteristic activity of senna usually occurs within 8-10 hours. So it is recommended to be used at
night. The long-term use of senna can cause excessive water and potassium loss because of
anthraquinones. Systemic corticosteroids with mineralocorticoid activity can cause water retention and
potassium loss too. Theoretically, prolonged use of senna combined with systemic corticosteroids may
increase the risk of hypokalaemia. Also, senna may increase the risk for digitalis toxicity including
cardiac arrhythmias due to its hypokalemic effect. Theoretically, in patients taking senna with
potassium-depleting diuretics cause excessive potassium loss. Also, laxative effect of senna reduces
furosemide absorption in the intestine. In a study with 7 patients with cardiac arrhythmias taking
sustained-release quinidine, senna reduced plasma quinidine levels, by about 25%. According to a case
report, a 45-year-old female patient who used senna-based laxative was hospitalized because of diffuse
abdominal pain. The patient was using warfarin regularly for six years. Blood tests showed a
haemoglobin concentration of 84 g/L; the platelet count was normal, but INR was 11.9; the activated
partial thromboplastin time was 92.4 s (control time 29.3 s). It has been reported that this effect was
due to decreased vitamin K (which leads to the activation of factors II, VII, IX and X) by the laxative
effect. Therefore, attention should be taken on concomitatnt use of warfarin with senna-based laxatives
to prevent risk of bleeding. In a study with 40 premenopausal women, the decrease in intestinal transit
time by senna significantly reduced serum oestrogen concentration. Theoretically, senna may reduce
the effect of estrogen-containing contraceptives. Experimental evidences suggest that danthron that is
anthraquinone found in senna reduces the absorptive permeability of ketoprofen by almost 30% and
the senna leaf infusion enhances ketoprofen permeability by about 1,5-fold. In conclusion, care should
be taken in patients using senna if patient has a concomitant drug use.

KEYWORDS
Senna, Drug interactions, laxative, sennosides

Corresponding Author: BURAK CEM SONER, burakcemsoner@gmail.com

1
NECMETTN ERBAKAN NVERSTES MERAM TIP FAKLTES TIBB FARMAKOLOJ AD.
2
KTO KARATAY NVERSTES TIP FAKLTES TIBB FARMAKOLOJ AD.

1246
 Poster Session 9
Submission ID: 1196

ANTIOXIDANT PROPERTIES OF FENUGREEK SEED

1 1
DAMLANUR KBRA AKIR , LKAY KOCA

ABSTRACT
Evaluating of natural antioxidant content of edible and medicinal plant materials are important
for human health and food industry. The aim of this study was to determine antioxidant activity of
fenugreek seed which is a spice and medicinal plant. Ten samples were analyzed in this study. The
samples were extracted with 40% ethyl alcohol solution, and total phenolics content, 1,1-diphenyl-2-
picrylhydrazyl (DPPH) free radical scavenging activity and ferric reducing antioxidant power (FRAP)
analyses were carried out in the extracts. Total phenolics, DPPH and FRAP values were found as
3925-5950 mg Gallic acid eqiuvalent (GAE)/100g (5125605 mg GAE/100g), 215.25-558.64 mol
TE/g (418.02107.55 mol/g) and 4139.07-9519.87 mol Fe2+/g (6800.871776.22 mol/g) in dry
matter, respectively. These results have showed that fenugreek seed is a good source in terms of
antioxidant.

KEYWORDS
Fenugreek seed, antioxidant, health

Corresponding Author: DAMLANUR KBRA AKIR, cakirdamlanurkubra91@gmail.com

1
ONDOKUZ MAYIS NVERSTES, MHENDSLK FAKLTES, GIDA MHENDSL BLM, 55139,
SAMSUN

1247
 Poster Session 9
Submission ID: 1197

A BIOACTIVE COMPOUND IN TEA: GAMMA-AMINOBUTYRIC

ACID (GABA)

1 1
EYDA BOSTANCI , ILKAY KOCA

ABSTRACT
Tea is a manufactured from the buds and leaves of Camellia sinensis. Tea contains many
chemical compounds such as catechins, alkaloids, polysaccharides, amino acids, vitamins, minerals,
and volatile oils. The free amino acids are particularly interesting because they are not only
responsible for the taste of tea infusions, but also have various beneficial effects. Gamma-
aminobutyric acid (GABA) is a four carbon free amino acid found in tea plant. GABA is produced by
the decarboxylation of L-glutamic acid that catalyzed by glutamate decarboxylase enzyme. There is
GABA in all tea types, but in GABA tea, the amount of GABA is much higher because of its special
production tecnique. GABA tea is known as Gabaron tea in Japan. GABA tea is manufactured by
fermenting fresh tea leaves under nitrogen gas. The taste of GABA tea is like oolong teas. GABA tea
is rich in antioxidants. GABA and GABA tea have numerous physiological functions and positive
effects on many metabolic disorders such as hypotensive effect, reducing anxiety and stress, helping to
modify sleep and mood, and alleviating postmenopausal depression. In this rewiew, the characteristics
and beneficial health effects of GABA and GABA tea are discussed.

KEYWORDS
Tea, aminoacid, GABA

Corresponding Author: LKAY KOCA, itosun@omu.edu.tr

1
ONDOKUZ MAYIS NVERSTES, MHENDSLK FAKLTES, GIDA MHENDSL BLM, 55139,
SAMSUN

1248
 Poster Session 9
Submission ID: 1198

ECHINACEA: PHARMACOLOGICAL PROPERTIES AND DRUG

INTERACTIONS

1 2 3 4 1
AYSE SAIDE AHIN , MEVRA AL , MEHMET MESUT PIKIN , HILAL KO SONER , BURAK CEM SONER

ABSTRACT
Echinacea is a group of American cone flowers from the Asteraceae (Compositae) family.
Within the class, there are nine plant species. Three of these are usually found in herbal preparations:
Echinacea purpurea (Purple Echinacea), Echinacea angustifolia and Echinacea pallida (Paleflowered
Echinacea). Although E. purpurea is the most commonly used spicy, it is frequently combined with E.
angustifolia. The active ingredients of the echinacea are polysaccharides, flavonoids, alkylamides,
polyacetylenes and essential oils. Polysaccharides seem to be responsible for the immunostimulatory
activity of the echinacea. The general preparations are prepared by fresh press or the ethanolic extract
of the dried parts of the fibres, roots and flowers of the plant. Glycerol is used instead of alcohol,
especially for children in flavoured liquid products. Echinacea increases the ability of granulocytes
and macrophages to ward off the disease. Activated macrophages secrete interleukin-1, interleukin-6,
and tumor necrosis factor-, which stimulates the specific immune system and protects the cells from
viral attacks. Caffeic acid derivatives such as chicoric acid and alkylamides are responsible for the
antiviral effect of purple echinacea juice. Also, they inhibit hyaluronidase, thereby reduces the
permeability of the blood vessels and inhibits the spread of local infection. Theoretically, echinacea
may antagonize the effects of immunosuppressant medication. Animal studies have shown that the
combination of powder extract of the root of E. purpurea and melatonin has an adverse effect on
mature granulocyte levels in bone marrow and spleen. A potentially positive interaction between E.
purpurea and phenytoin has been reported in mice. Phenytoin is known to increase the risk of cleft
palate when used in pregnancy. According to this study, E. purpurea decreased the incidence of cleft
palate caused by phenytoin. E. purpurea root has been shown to selectively modulate CYP3A activity
in intestine (inhibition) and liver (induction) in a clinical study. This biphasic effect of E. purpurea
root has been attributed to different ingradients in root content, differences in absorbtion rate of
ingradients or a systematically formed metabolite. Echinacea purpurea root alters the metabolism of
the probe drugs by the inhibition of intestinal CYP3A and hepatic CYP2C9 and CYP1A2. However, it
has been shown that 400 mg Echinacea purpurea root treatment for 8 days resulted with hepatic
CYP3A induction and increases clearance of intravenous midazolam. Echinacea may increase oral
clearance of CYP3A substrates with a low clearance and high oral bioavailability such as alprazolam
because of the hepatic induction of CYP3A. Conversely, for CYP3A substrates such as buspirone,
simvastatin, and terfenadine, which have low oral bioavailability due to first-pass metabolism in the
intestine, may result in increased serum concentrations of these substrates as a result of intestinal
CYP3A inhibition when used with echinacea. Although details of the echinacea genus and its extract
used are not specified, it has been reported that there may be an interaction between Echinacea and

Corresponding Author: BURAK CEM SONER, burakcemsoner@gmail.com

1
NECMETTN ERBAKAN NVERSTES MERAM TIP FAKLTES TIBB FARMAKOLOJ AD.
2
KTO KARATAY NVERSTES TIP FAKLTES TIBB FARMAKOLOJ AD.
3
NECMETTN ERBAKAN NVERSTES MERAM TIP FAKLTES ROLOJ AD.
4
KONYA DR.FARUK SKAN DOUM VE OCUK HASTANES

1249
etoposide (CYP3A4 substrate) with a case report. A 61-year-old male patient was diagnosed with cell
lung cancer and chemoradiotherapy was initiated simultaneously with cisplatin and etoposide. He was
admitted to the clinic on the 8th day of her first recurrence because of thrombocytopenia. Platelet
transfusion support was given. Although researchers have not confirmed the interaction between
echinacea and etoposide, they concluded that warnings should be given to the use of CYP3A4
substrates (antracyclines, etoposide, vinca alcaloids, taxanes) and echinacea in patients receiving
chemotherapy. Theoretically, chemotherapeutics etoposide, epipodophyllotoxin, cyclophosphamide,
ifosfamide, vindesine, vinblastine, vincristine, vinorelbine, paclitaxel, docetaxel, irinotecan,
tamoxifen, tipifarnib which are metabolized by CYP3A4 and dacarbazin which is metabolized by
CYP1A2 may also interact with echinacea.

KEYWORDS
Echinacea, drug interactions, CYP3A4 induction

1250
 Poster Session 9
Submission ID: 1199

GINKGO BILOBA: PHARMACOLOGICALPROPERTIES AND DRUG

INTERACTIONS

1 2 2 2
MEVRA AL , AYE SAIDE AHIN , ESER YILDIZ , BURAK CEM SONER

ABSTRACT
Ginkgo biloba, also known as a fossil tree, is the oldest living tree and leaf extracts have been
used for centuries in the treatment of various diseases. In Chinese medicine tea is prepared for asthma
and bronchitis treatment. In Germany and other European countries, it is frequently prescribed in form
standard tablet or capsule form. Its major components are flavonoids, proanthocyanidins, terpene
lactones including ginkgolide A, B, C and sesquiterpene bilabolides. The standardized Ginkgo extract
contains 22-27% flavone glycosides and 5-12% terpene lactones which are main active ingradients.
Flavonol glycosides, a component of the ginkgo leaf, improves blood circulation. Ginkgolid-B is a
platelet activating factor antagonist. The main indications for Ginkgo are primary degenerative
dementia, vascular dementia, absent state, confusion, lack of energy, fatigue, decreased physical
performance, depressive mood, anxiety, vascular and dizziness and ear tinnitus, relieving the
symptoms caused by the peripheral arterial occlusive disease. The most important interaction with
Ginkgo biloba is the risk of spontaneous bleeding that can be observed when used with anticoagulants.
A number of clinically significant cases of bleeding have been reported. A 70-year-old man started
using 40 mg of standardized Ginkgo biloba extract twice a day. After one week he complained of
recurrent blurred vision attacks in his right eye. He went to the clinic 2 days later and a red color
change in the cornea was noticed. The only medication the patient used was a single dose of 325 mg
aspirin, which is prescribed after coronary artery bypass surgery since 3 years. The patient did not
have any eye trauma, ischemia or vascular occlusion in the past. The physical examination was
entirely normal except for a fine stream of blood oozing down from the 12 o'clock position of the
margin of the iris into the inferior angle, where a layering of blood was seen on gonioscopy. The
patient stopped taking Ginkgo's extract but continued aspirin treatment. It has been reported that the
bleeding did not recur during the three-month follow-up period. The injection of Gingko biloba extract
to increase microcirculation due to anticoagulant effect with sodium aescinate obtained from horse
chestnut seed approved by Chinese Food and Drug Administration for postoperative oedema treatment
has resulted with severe nephrotoxicity. It has been reported that fetal intracerebral hemorrhage
develops in a patient using nonsteroidal anti-inflammatory agent containing ibuprofen with Ginkgo
biloba extract. The effect of Gingko biloba (daily 100 mg extract) was investigated with a placebo-
controlled cross-blind study involving 21 patients receiving prolonged warfarin treatment, and it
turned out that INR value did not change. Experimental studies show that Ginkgo biloba accelerated
the onset of ototoxicity caused by amikacin and increases ototoxic adverse effect. Ginkgo biloba also
increases the extrapyramidal adverse effects of haloperidol. Ginkgo biloba has been reported to cause
priapism in a patient receiving risperidone treatment. The -adrenergic properties of risperidone cause

Corresponding Author: BURAK CEM SONER, burakcemsoner@gmail.com

1
KTO KARATAY NVERSTES TIP FAKLTES TIBB FARMAKOLOJ AD.
2
NECMETTN ERBAKAN NVERSTES MERAM TIP FAKLTES TIBB FARMAKOLOJ AD.

1251
priapism very rarely. Possible vascular effects of Gingko biloba may increase the adverse effects of
risperidone. Gingko biloba increases omeprazole metabolism by CYP2C19 enzyme induction and
similarly enhances the metabolism of other proton pump inhibitor drugs which are also CYP2C19
substrate. It has been reported that co-administration with efavirenz results with virological failure.
According to researchers, terpenoid ingradients in Ginkgo biloba causes CYP3A4 or P-glycoprotein
induction. However, clinical trials have shown that Gingko biloba does not cause significant changes
in the CYP1A2, CYP3A4, CYP2D6, or CYP2E1 enzyme phenotype. It has been reported that a 55-
year-old female patient with glucose-6-phosphate dehydrogenase deficiency who has 17.5 mg of
Ginkgo biloba leaf extract injection for dementia prophylaxis without doctor's advice developed
hemolytic anemia. In 2013, the Ginkgo biloba extract by the International Agency for Research on
Cancer has been classified as possibly carcinogenic to humans (Group 2B).

KEYWORDS
Ginkgo biloba, drug interactions, ginkgolide,

1252
 Poster Session 9
Submission ID: 1200

GARLIC: PHARMACOLOGICAL PROPERTIES AND DRUG

INTERACTIONS

1 2 1 3 1
ESER YILDIZ , MEVRA AL , AYE SAIDE AHIN , TARIK ACAR , BURAK CEM SONER

ABSTRACT
Allium sativum (garlic) have been used for medicinal purposes for a century. The active
compounds of garlic include alliin, allinase, diallyldisulfide, ajoens. Alicin is major garlic constituent,
which is also responsible for the aroma of fresh garlic, sulphur-like smell. When fresh garlic is
chopped or crushed, the enzyme alliinase converts alliin into allicin, The pharmacological effects of
garlic are mainly linked to sulphur-containing components. Other sulphur compounds found in garlic
are allylmethyltrisulfide, allylpropyldisulfide, diallyldisulfide, diallyltrisulfide, ajoene. It also contains
glycosides, monoterpenoids, enzymes, flavonoids based on kaempferol and quercetin. Aged garlic
extract (AGE) that prepared with the long-term extraction of fresh garlic (normally at 20 months)
appears to be superior to normal garlic in its antioxidant properties. The most common known
pharmacological effect is lowering total serum cholesterol levels, probably through the inhibition of
cholesterol synthesis in the liver. Garlics other pharmacological effects are its antibacterial, antiviral,
antifungal, antihypertensive, hypoglycemic, antithrombotic, antimutagenic, and antiplatelet effects.
Also in vitro studies have demonstrated that both garlic powder and garlic extract inhibit human
lymphatic leukaemia cell growth. Garlic has been reported to have hepatoprotective effect against
carbon tetrachloride induced acute hepatotoxicity model in rats. Interactions between garlic and
warfarin (oral anticoagulan agent) may be clinically significant. Garlic reduces platelet aggregation
and it may cause spontaneous bleeding even when used alone. Therefore, garlic may cause bleeding if
concomitantly used with anticoagulant and non-steroidal anti-inflammatory drugs. Clinical studies
showed that liquid, aged garlic extract inhibits platelet aggregation. Also, animal studies showed that
ajoene and sulphur compounds derived from garlic possess antiplatelet and antithrombotic property.
Studies suggest that AGE inhibits platelet aggregation via inhibition of GPIIb/IIIa receptor thus
inhibits the binding of fibrinogen to platelet. Garlic ingestion should be stopped at least one week
before surgeries. Although drug interaction of garlic and lisinopril has been associated significant
hypotension and syncope has been reported, no interaction has been shown. When garlic is used with
fish oil, it reverses the increasing effect of fish oil on low-density lipoprotein cholesterol (LDL) levels.
Garlic supplements reduces LDL levels and fish oil reduces triacylglycerol levels but increases LDL
levels. Combined use may be more beneficial in patients with high blood lipids. The effect of crude
aqueous extract of garlic on the pharmacokinetic parameters of isoniazid and rifampicin was studied in
rabbits. While oral administration of the garlic extract did not cause any changes in the
pharmacokinetic parameters of rifampicin, it significantly reduced isoniazid bioavailability without
causing any change in elimination rate. Crude aqueous extract of garlic reduced AUC and maximum
serum levels of a single 30-mg/kg dose of isoniazid by about 55% and 65%, respectively, when

Corresponding Author: BURAK CEM SONER, burakcemsoner@gmail.com

1
NECMETTN ERBAKAN NVERSTES MERAM TIP FAKLTES TIBB FARMAKOLOJ AD.
2
KTO KARATAY NVERSTES TIP FAKLTES TIBB FARMAKOLOJ AD.
3
KONYA BEYHEKM DEVLET HASTANES

1253
compared with the levels after a single 30-mg/kg dose isoniazid. The use of garlic with protease
inhibitor saquinavir may significantly reduce saquinavir plasma levels. The mechanism of this
interaction is uncertain but garlic may be reducing saquinavir bioavailability by increasing its
metabolism in intestine. Care should be taken on concomitant use of garlic with other CYP3A4
substrate protease inhibitors such as amprenavir, atazanavir, darunavir, fosamprenavir, indinavir,
lopinavir, nelfinavir, ritonavir, tipranavir.

KEYWORDS
garlic, allium sativum, drug interactions

1254
 Poster Session 9
Submission ID: 1201

THE MEDICINAL AND AROMATICAL PLANT POTENTIAL IN

TRKIYE AND CONSERVATION STUDIES

1
BELKIS MUCA

D
ABSTRACT
It is known that approximately 12,000 plant taxa have been distributed according to the data

TE
obtained in recent years in Turkey. It has been determined that up to 500 of these plants (about 300 are
sold in import shop) are medicinal and aromatic plants. However, it is noteworthy that the number of
medicinal and aromatic plants of an country rich in plant diversity is so little. This study is prepared to
show the diversity of medical and aromatic plants in our country, the areas they spread and the
conservation studies suggested for these plants. In our country, medicinal and aromatic plants show
EN
more spread in less soil, rocky and especially in steppe fields. The more challenging the habitat
conditions, the higher the amount of essential substance in medical and aromatic plants. For this
reason, there are some clustering points for these plants in our country. Eastern Anatolia, Southeastern
Anatolia and the Lakes Region are some of these points. The commercial importance and diversity of
ES
medical and aromatic plants in our country is also remarkable. Although commercially valuable
medicinal and aromatic plants are cultivated in some regions, natural collection in the whole country
takes place at a higher rate. This jeopardizes both our plant diversity and our medical and aromatic
potential. The model studies of the Ministry of Forestry and Water Affairs are inadequate with their
existence. In our work, methods are proposed to evaluate the natural distribution of medicinal and
PR

aromatic plants. The work is expected to provide a source for medical and aromatic plant collectors,
traders, healthcare users and researchers.

KEYWORDS
T

Medical Plant, Aromatic Plant, Distribution, Conservation, Trkiye.

O
N

Corresponding Author: BELKIS MUCA, belkismuca@gmail.com

1

1255
 Poster Session 9
Submission ID: 1202

CYTOTOXIC ACTIVITY OF THE ROOT AND FRUIT EXTRACTS OF

NEOCRYPTODISCUS PAPILLARIS (BOISS.) HERRNST. & HEYN

1 1 2
FATMA TOSUN , FEYYAZ MIHOLUGL , MAHMUT MSK

ABSTRACT
The aim of the present study is to investigate the cytotoxic activity of Neocryptodiscus
papillaris (Boiss.) Herrnst. & Heyn (Apiaceae). The roots and fruits of N. papillaris were collected
from Aa Dilimli village, Viranehir, anlurfa in June 2013. The plant materials were separately
and sequentially extracted with dichloromethane and methanol at room temperature. The extracts were
individually concentrated in a rotary evaporator under reduced pressure to dryness. Furthermore,
methanol extract was dissolved in methanol/water (10:90) and then partitioned with ethyl acetate in a
separatory funnel, the resulting extracts were separately concentrated in vacuo to dryness. These
extracts were subjected to the cytotoxic activity testing. The highest activity was found in the
dichloromethane extracts against the colon cancer COLO205 and KM12 cell lines. The
dichloromethane extract of the root of N. papillaris showed cytotoxic activity with IC50 values of 23.0
and 14.0 ug/mL against the colon cancer COLO205 and KM12 cell lines, respectively. Whereas, the
dichloromethane extract of the fruit showed cytotoxic activity with IC50 values of 23.1 and 17.2
ug/mL against the colon cancer COLO205 and KM12 cell lines, respectively.

KEYWORDS
Neocryptodiscus papillaris, cytotoxic activity

Corresponding Author: FEYYAZ MIHOLUGL, feyyazmihoglugil@gmail.com

1
GAZ NVERSTES ECZACILIK FAKLTES FARMAKOGNOZ ANABLM DALI
2
STANBUL NVERSTES ECZACILIK FAKLTES FARMAKOGNOZ ANABLM DALI

1256
 Poster Session 9
Submission ID: 1203

ROSEMARY: PHARMACOLOGICAL PROPERTIES AND DRUG

INTERACTION

1 2 1 3 1
ESER YILDIZ , MEVRA AL , AYE SAIDE AHIN , DURMU ALI ASLANLAR , BURAK CEM SONER

ABSTRACT
Rosmarinus officinalis (rosemary) is grown in the South and West Anatolian. The plant
belongs to the family of Labiatae (Lamiaceae) which is a strong aromatic low evergreen shrub.
Constituents include essential oil (1,8 cineole, -pinene and camphor), diterpenes, rosmarinic acid that
derivatives from caffeic acid, flavonoids and triterpenes. Rosemary is used for flavouring foods and in
cosmetic products. In traditional medicine it is used as an antispasmodic in renal colic and
dysmenorrhoea, in relieving respiratory disease, in supportive treatment of rheumatic disorder and for
stimulating hair growth. Rosemary oil may irritate the skin and increases the circulation when applied
topically. Major bioactive constituents of rosemary oil are rosmarinic acid, carnosolic acid and
carnosol which are responsible from antioxidant, anti-inflammatory and anticarcinogenic effects.
Rosemary oil possesses antibacterial and antifungal properties. Rosemary is an effective antimicrobial
against Staphylococcus aureus when used as spices. It has been shown that the lyophilized aqueous
extract of rosemary shows hepatoprotective effect by reducing t-butyl hydroperoxide induced
malonaldehyde formation in rat hepatocytes in vitro. Also, it significantly decreases of the release of
hepatic lactate dehydrogenase and aspartate aminotransferase. Carnosol, carnosolic acid and rosmanol
in the diterpene structure of the lipophilic and hydrophobic fractions of rosemary shows inhibitory
activity on superoxide anion production in the xanthine/xanthine oxidase system. The spasmolytic
effect of 1,8-cineole and bornyl acetate in the ileum smooth muscle and atrium muscle was
demonstrated in animal studies. It has been shown that rosemary extract treatment significant increases
the uptake of radiopharmaceutical agent technetium-99m (99mTc) Sulphur Colloid in rat liver. These
results indicate that rosemary extract or metabolites changes the biodistribution of 99mTc-Sulphur
Colloid which is a commonly used radionuclide for diagnostic radiopharmaceuticals. The ethanol
extract of rosemary and cefuroxime combination show synergistic activity against methicillin-resistant
Staphylococcus aureus. It is speculated that the bioactive component in rosemary damages the
cytoplasmic membrane of the bacteria and thus facilitates antibiotic influx. The rosemary is rich in
phenolic acid and can cause a reduction in non-heme-iron absorption. Rosemary inhibits the binding
of doxorubicin, vinblastine and azidopine to P-glycoprotein (P-gp) thus directly inhibits membrane P-
gp efflux activity. This increases the intracellular accumulation of medicines by reducing P-gp
mediated efflux. Theoretically, it is possible that rosemary can change the plasma levels of drugs that
are P-gp substrates such as aliskiren, ambrisentan, colchicine, dabigatran, digoxin, everolimus,
fexofenadine, imatinib, lapatinib, maraviroc, nilotinib, posaconazole, ranolazine, saxagliptin,
sirolimus, sitagliptin, talinolol, tolvaptan, topotecan. Animal studies shows that rosemary has
significant in vitro and in vivo antithrombotic activity. The antithrombotic mechanism can be

Corresponding Author: BURAK CEM SONER, burakcemsoner@gmail.com

1
NECMETTN ERBAKAN NVERSTES MERAM TIP FAKLTES TIBB FARMAKOLOJ AD.
2
KTO KARATAY NVERSTES TIP FAKLTES TIBB FARMAKOLOJ AD.
3
UMRA DEVLET HASTANES

1257
explained by the direct inhibitory effect on platelets. Animal studies showed that oral rosmarinic acid
reduces fibronectin and fibrin formation in the glomerulus. Theoretically, concurrent use of
anticoagulant medications may increase the risk of bleeding. According in-vitro studies, the aqueous
extract of rosemary can inhibit the angiotensin converting enzyme. In animal studies, it has been
shown that rosemary has diuretic effects and lowers electrolyte levels. It has also been shown to
increase permeability of furosemide in vitro in CaCO2 monolayer cell-line. According to the case
reports, rosemary treatment can induce lithium toxicity due to its diuretic effect.

KEYWORDS
Rosmarinus officinalis, rosemary, drug interactions,

1258
 Poster Session 9
Submission ID: 1204

CHEMICAL COMPOSITION AND ANTIOXIDANT ACTIVITY OF

ESSENTIAL OIL OF ORIGANUM SIPYLEUM

1 2 1 1 2
MELTEM TA , SELUK KKAYDIN , IDEM KU , MEHMET EMIN DURU , HASAN YILDIRIM

ABSTRACT
Turkey has a rich flora, in terms of, medicinal and aromatic plant diversity. The genus
Origanum is a member of Lamiaceae family and represented by 32 taxon in Turkey and 21 of them are
endemic for Turkey. Origanum sipyleum is an endemic species to Anatolia. It used as herbal tea, spicy
and folk medicine by people in Turkey The purpose of this study was investigate to the chemical
composition and antioxidant activties of the essential oil of Origanum sipyleum. The plant was
provided from Mula in 2015. For obtain essential oil, aerial parts of O.sipyleum was hydrodistillated
for 3h using a Clevenger type equipments.The essential oil was analysed by using GC-GC/MS.
Antioxidant activities of extracts were performed by five complementary tests systems i.e. -carotene
linoleic acid, DPPH free radical scavenging, ABTS cation radical scavenging, cupric reducing
antioxidant capacity (CUPRAC) and metal chelating assays. Twenty five components were identified
by GC-GC/MS in essential of O.sipyleum.-caryophyllene (22.2 %) , -terpinene (18.3%), carvacrol
(8.8%) were found the major components of O. Sipyleum. According to antioxidant activity results,
O.sipyleum exhibited, 63.851.54%, 12.171.01%, 46.151.04% inhibitions and 0.380.05
absorbance value in -carotene linoleic acid, DPPH free radical scavenging, ABTS cation radical
scavenging and cupric reducing antioxidant capacity (CUPRAC) assays respectively at 800 g/ml
concentration.

KEYWORDS
Antioxidant activity, essential oil ,GC-MS, Origanum sipyleum

Corresponding Author: MELTEM TA, tasmeltem@hotmail.com

1
MULA SITKI KOMAN NVERSTES
2
EGE NVERSTES

1259
 Poster Session 9
Submission ID: 1205

HERBAL MEDICINE IN CHRONIC RENAL DISEASES

1
MER ARSLAN KALAFATCILAR

ABSTRACT

D
Chronic renal dieases are important causes of morbidity and mortality. Here we report a case
with glomerulonephritis who was treated with herbal medicine. A 62-year-old man was admitted to
Nephrology Clinic with edema of lower extremities. Laboratory investigations and renal biopsy

TE
showed 'Membranoproliferative glomerulonephritis' (MPGN) disorder. His protein levels of 24-hour
urine ranged from 8 to 15 gr/day under the treatment of diuretics and statins. An 2% infusion of
Solidago virgaurea L. was given three times a day. At the fourth month of herbal infusion treatment,
his 24-hour urine analysis for proteinuria was reduced to 2,5 gr/day. Thuj Q 6 homeopathy treatment
EN
was added after herbal infusion therapy and 24-hour urine analysis for proteinuria showed decreased
protein levels to 1,5 gr/day. His clinical course is stabile for 13 years without any medical treatment.
In conclusion chronic renal diseases have progressive course and herbal medicine and homeopathy are
rarely being used in these disorders in Turkey. The aim of this case report is to point to the usage of
Solidago virgaurea L. and homeopathy as an alternative treatment modality for MPGN disorder.
ES
Educational training programs need to be added in medicine schools in our country. By this way,
further researchs will be done and these patients with chronic renal disease will have chance to use
alternative treatment modalities like herbal infusions or homeopathy.
PR

KEYWORDS
Glomerulonephritis, edema, Solidago virgaurea L.
T
O
N

Corresponding Author: MER ARSLAN KALAFATCILAR, oakalafatcilar@yahoo.com

1
CELAL BAYAR NVERSTES, ALAEHR MESLEK YKSEKOKULU, TIBB VE AROMATK BTKLER
PROGRAMI (EMEKL RETM YES)

1260
 Poster Session 9
Submission ID: 1206

DETERMINATION OF ANTIFUNGAL EFFECTS OF THYME,

ROSEMARY AND RED PEPPER AGAINST ASPERGILLUS NIGER,
PENICILLUM SSP. AND MUCOR SSP.

1 1 1
ASLI ELKEL , MUTLU BUKET AKIN , BRA GNC

ABSTRACT
In addition to the taste and flavor characteristics herbal in the world is used for the treatment
and protection during the long years. Herbal extracts and essential oils, which are derived from plants
by various methods, has been proved by scientific studies to have antimicrobial effects. Thyme is a
kind of spice that is widely used in food industry. Its composition contains 63.9% carbohydrate, 9.1%
protein and 7.4% fat. It has been reported that thymol, carvacrol, p-cymene, -pinene and camphene
found in thyme essential oil, phenolic compounds and flavonoids of thyme have antimicrobial and
antioxidant activity. The composition of rosemary (Rosmarinus officinalis), which has widespread use
as a natural preservative, is 64.1% carbohydrate, 15.2% fat and 4.9% protein convenience. Rosemary
essential oil generally contains 1,8-cineole, -pinene, camphor, camphene, borneol, piperitone, linalol.
Antimicrobial, antioxidant and antiviral effects of rosemary have been determined by many studies.
Red pepper is a kind of plant species that grows annually depending on the genus Capsicum of the
Solanaceae family. It is rich in ascorbic acid and carotene contents. Generally it is used as spice,
feedstuff and antibiotic raw material in pharmaceutical industry. Mold is one of the types of
microorganisms that can cause food spoilage. Some mold species produce secondary metabolites
called "mycotoxins" that show toxic properties. Aspergillus, Penicillum, Alternaria, Fusarium, and
Mucor are the most common molds that produce toxins In this study antifungal effects of mixtures of
extracts and essential oils of thyme, rosemary and red pepper on Aspergillus nigeri, Penicillum ssp.
and Mucor ssp. were investigated. According to the results, the highest antifungal effects were
determined from the mixture of rosemary extract and rosemary oil against Mucor ssp. and from the
mixture of thyme extract and thyme oil against Aspergillus niger.. It was also found that the lowest
antifungal effect was shown from red pepper extract and rosemary extract and oils against Penicillium
ssp.

KEYWORDS
Thyme, Rosemary, Red Pepper, Antifungal effect

Corresponding Author: ASLI ELKEL, acelikel2@gmail.com

1
HARRAN NVERSTES, ZRAAT FAKLTES, GIDA MHENDSL BLM, ANLIURFA

1261
 Poster Session 9
Submission ID: 1208

SAFFRON (CROCUS SATIVUS L. ): ITS COMPOSITION AND

CLINICAL PROPERTIES

1 2 3 3
HAVVA ARIN , GKHAN DEGE , BIRSEN YILMAZ , GAMZE AKBULUT

ABSTRACT
In our country, Crocus Sativus L. is known with different names such as saffron, saffron
flower, saffron crocus and zagferan. It belongs to the family of Iridaceae as well as it grows naturally
in India, the Balkans, and the Eastern Mediterranean, a perennial, 20-30 cm long bulb with a purple
flower blooming in the autumn. Besides carbohydrates, polypeptides, lipids, H2O, minerals and
vitamins, there are more than 150 different compounds in saffron. Also, it contains three main
bioactive components: crocin, safranal and picrocrocin. Crocin, a water-soluble carotenoid, is the
glycosyl ester of crocetin and consists of two sugars responsible for the color of the compound, giving
a typical reddish-yellowish color to safran. Safranal is a monoterpenic aldehyde that is responsible for
the characteristic aroma and odor of the saffron, and the bitter taste of the saffron is due to picrocrocin,
which is the safranal glycoside. Saffron is a plant mostly used in Asia for digestion, inflammation and
cerebral disorders. Positive anxiolytic and antidepressant-like effects of this plant have also been
reported. This effect is due to safranal and crox. As a result of clinical trials, daily dosage of saffron
extract used as a supplement in the treatment of depression is 30 mg. Satieral, another component of
saffron, is effective in weight management. In the studies, it was observed that the effects of satieral
improved mental condition, increased satiety feeling and decreased snack feeling. It is therefore
argued that a combination of a balanced diet and satieral supplementation in weight loss programs
may help achieve the target weight. However, studies on this issue are insufficient and more research
is needed. Despite the recent information about properties of saffron, the mechanisms for reducing the
feeling of snacking remain speculative. It is expected that the central and/or environmental factors
involved in neuropharmacology will be clarified in order to explain this effect. The anti-cancer
properties of saffron were also investigated and substantial results were obtained. However,
researchers say that the dose used during treatment should be doctor-controlled to prevent food-drug
interactions in chemotherapy. Apart from such health effects, saffron has many other therapeutic
effects. There are positive impacts on the digestive, cardiovascular, immune, respiratory, genital-
urinary and central nervous system. In addition to these, it has a role in radical scavenging, genetic
protective properties, inhibition of cell proliferation, inhibition of lipid peroxidation and anti-
inflammatory processes. Saffron is used as a prophylactic and therapeutic agent against
gastrointestinal system disorders. However, further clinical trials are required to confirm these effects.

KEYWORDS

Corresponding Author: BRSEN YILMAZ, dytbirsen@gmail.com

1
ZEL SONDURAK MRAN TIP MERKEZ
2
ALANYA TOPLUM SALII MERKEZ
3
GAZ NVERSTES

1262
Saffron, Crocus Satvus L., health, crocin

1263
 Poster Session 9
Submission ID: 1211

DETERMINATION OF FACTORS AFFECTING ON AVAILABLE

ALTERNATIVES IN MEDICINAL NEEDS OF STUDENTS: CASE
STUDY OF ATATRK UNIVERSITY

1 1 1
AHMET SEMIH UZUNDUMLU , MEHMET MUHAMMED SARI , ZEINAB MOKHTAR, AYE SEZGIN

ABSTRACT
From time immemorial, medicinal aromatic plants have been one of the important treatment
methods. People of our time because of their medicinal needs or in order to protect themselves from
diseases alongside with going to a doctor or taking medicine without seeing a doctor refer to using
medicinal plants. Similar situation is observed in university students too. The aim of this paper is
determining factors influencing our future, university students', preferring one method over the other
two when they have health problems. To achieve this aim, 445 students from 15 faculties representing
Atatrk University were interviewed. Tobit method was used for identifying factors influencing
student's choise of one method. It is necessary to do works for informing young people according to
the results, obtained from this study.

KEYWORDS
Atatrk University, Students, Medical needs, Tobit

Corresponding Author: AHMET SEMH UZUNDUMLU, asuzsemi@atauni.edu.tr

1
TARIM EKONOMS

1264
 Poster Session 9
Submission ID: 1213

CHAMOMLE TEA; POTENTIAL EFFECTS ON HEALTH

1 1 1 1
HILAL BETL ALTINTA , NILFER ACAR TEK , GAMZE AKBULUT , TESLIME ZGE YRSN , BRA
1
ATABLEN

ABSTRACT
Chamomile tea is often preferred by consumers for consumption in tea. It is stated that
chamomile was used in various medical treatments by ancient Egyptians, Greeks and Romans for
thousands of years. Chamomile is said to be used for various skin diseases such as acne, eczema,
colds, sore throat, abscess, gum inflammation, anxiety and mild burns. However, there is very little
work done to determine this effect of the chamomile. In some studies in rats, it has been determined
that chamomile is anti-inflammatory and anti-oxidant. There are some studies showing that it is
effective in reducing the symptoms of mild to moderate severe anxiety disorder. Chamomile spread
from Egypt to Europe. German chamomile are most commonly used in tea, with varieties such as
German and Roman chamomile. The German chamomile tea contains apigenin, a flavanoid known to
be a mild sedative effect. Chamomile flowers contain a large number of therapeutically active
compounds, mostly categorized according to their polarities. The most important bioactive
components are flavonoids. Anti-inflammatory effect of German chamomile is used to increase
immunity against cancer, gastritis, chronic intestinal diseases. It also determined the effects of anxiety
disorders and increased sleep quality. Camellias combined with valerian are a traditional plant that has
been used since ancient times in the treatment of insomnia and many health problems. The chamomile
flower has tea, extract and various forms of ointment. Chamomile is generally known as anti-
inflammatory and emotional conditioner. Despite the calming effect of the calamus in studies, studies
are needed to determine its effect on sleep quality.

KEYWORDS
chamomile tea, health

Corresponding Author: HLAL BETL ALTINTA, hilalbetulaltintas@gmail.com

1
GAZ NVERSTES SALIK BLMLER FAKLTES

1265
 Poster Session 9
Submission ID: 1214

LAMB BREAST MUSHROOM

1 1
MUSTAFA DEMRBA , REFIK ULUSOY

ABSTRACT
The Latin name is Morchella esculenta, a lamb breast mush egg-like hat with reddish yellow
and irregular hollows. The lamb breast collected from the nature from the middle of spring is one of
the most important fungus species exported in Turkey. It is a delicious mushroom species widely
consumed in Turkey and in the world. It can be consumed fresh or dried. What kind of mantle is a jug?
Lamb, which loves soils rich in nutritional components, grows in the openings of forests, on the sides
of roads and water, in wildfires, under trees like maple, elm, hazelnut and beech. It is seen in the
Aegean and Mediterranean regions, in the forested land, usually in the pine forests. The cause is not
yet known but it is the most common type of fungus after forest fires. The growing season is between
March and May. Morchella conica: It is a different breed with brown hats and regular cavities that are
very similar to the lamb chest. In some regions, as a lamb breed, the genus is also the genus Morchella
esculenta. Also known as lamb mushrooms, donut mushrooms. It grows in calcareous soil, in
meadows and in bushes. It meets in April and May. How are lamb breasts grown? The fungi belonging
to the genus Morchella belong to the mycorrhiza class. The ability of such fungi to survive depends on
the presence of some trees and organic compounds. For this reason, despite the fact that they have
been working on them for many years, the results of their cultural studies have not been successful.
Culture studies of the lamb breast, which was first cultured in 1982, are carried out in order to increase
the population of fungi in the natural environment today. BENEFITS - Kollestrole is good - Supports
eye health - Supports thyroid health - Alleviate joint pain - Maintain side effects of drugs - Iron and
phosphorus source -B and D are sources of Vitamin Supports liver health - Fights the cancer -He
makes blood -The penalty is good Supports heart health

KEYWORDS
Lamb breast mushroom

Corresponding Author: MUSTAFA DEMRBA, mustafademirbas@ogm.gov.tr

1
ORMAN GENEL MDRL

1266
 Poster Session 9
Submission ID: 1215

DRYING PROCESS IN MEDICINAL-AROMATIC PLANTS

1 1
RABIA SERPIL GNHAN , ASUMAN KAN

ABSTRACT
DRYING PROCESS IN MEDICINAL-AROMATIC PLANTS Gnhan, R. S.1, Kan, A.1 1
Selcuk University, Vocational Higher School of Technical Sciences, Food Processing Department
42070, Campus, Konya rsgunhan@selcuk.edu.tr Phone:+90 533 6366616 Medical - Aromatic plants
(TAB) constitute the most important inputs of herbal medicines, plant chemicals, food and additives,
cosmetics and perfumery. These plants need to be in the required quantity and quality so that
sustainable production and market potential can be adequately evaluated. One of the important process
steps for quality and standard product is drying process after harvest. Drying is the heat and mass
transfer event involving the transfer of the water in the dried product from the interior of the product to
the surface and the evaporation from the surface. Drying is done to preserve the enzymatic and
microbial activities and to extend shelf life of the product. Drying is to reduce the moisture of the
product without harming the product quality to the final moisture content as soon as possible by
consuming the least energy. Drying can be done naturally and artificially. The methods and conditions
used in drying directly affect the quality of the product and the content of the active substance. In
aromatic plants, the drying temperature should be kept low to prevent the loss of essential oil. For
quality drying, thick and large drugs should be cut into pieces, branch pieces should be selected. One
of the major risks during the drying of drugs is the occurance of microbial activity, especially the
formation of mycotoxin that is harmful to human health in the final product. In this context, the use of
controlled drying systems is recommended. In addition, freeze drying is a recommended method for
some drugs with low production volume and high economic value. As a result; The correct drying
method should be selected and the correct drying conditions should be applied in order to have the
product of the closest composition (phenolic compounds, volatile oils, resins, flavorings, colorants,
vitamins, antioxidants and antimicrobial compounds etc.)to the fresh product after drying. Key words:
Drying, Medicinal and Aromatic Plants, Preservation

KEYWORDS
Drying, Medicinal and Aromatic Plants, Preservation

Corresponding Author: RABA SERPL GNHAN, rsgunhan@selcuk.edu.tr

1
SELUK NVERSTES

1267
 Poster Session 9
Submission ID: 1216

THE ROLE OF HAWTHORN IN THE DEVELOPMENT OF

FUNCTIONAL FOOD

1 1
RABIA SERPIL GNHAN , SUZAN YALIN

ABSTRACT
THE ROLE OF HAWTHORN IN THE DEVELOPMENT OF FUNCTIONAL FOOD
Gnhan, R. S.1, Yaln, S.2 1 Selcuk University, Vocational Higher School of Technical Sciences,
Food Processing Department 42070, Campus, Konya 2 Selcuk University , Department of Food
Hygiene and Technology, Faculty of Veterinary Medicine , Campus, Konya rsgunhan@selcuk.edu.tr
Phone:+90 533 6366616 Hawthorn is an important Medicinal-Aromatic plant that attracts the attention
of the scientific world. In developed countries, the production and consumption of functional food and
beverage whose added-value are high are gradually increasing by using of fruits, leaves and flowers of
hawthorn. With the investigations it has been found that hawthorn has many functional properties and
has become one of the most popular herbal treatment tools. It is used in the treatment of disorders such
as heart failure, vascular occlusion, angina, dysrhythmia, gastrointestinal disorders and hypertension.
The hawthorn is rich in Vit C, sugars (fructose, glucose, sucrose), organic acids (malic acid, citric
acid, quinic acid), anthocyanins, flavonoids and phenols. Also hawthorn fruit contains high amounts of
mineral matter. Over the world, hawthorn is used in the production of fresh fruit, dried fruit, juice,
compote, marmalade, jam, crush, tea (fruit, leaf, flower), molasses, vinegar, sauce, powder of
hawthorn; cakes, biscuits, confectionery and ice cream. It is stated that consuming hawthorn powder
reduces significantly the level of low density lipoprotein in the body. The hawthorn vinegar contains a
high amount of phenolic material and has high antioxidant capacity. Especially t has a positive effect
on cardiovascular patients. In addition, the hawthorn vinegar causes weight loss and reduces the blood
pleasure, cholesterol, blood sugar, triglycerides. Dried leaves, flowers and fruits of the hawthorn are
prepared like tea and it is used against throat inflammation, coughing, heart attack and arteriosclerosis.
Finger hawthorn and soudjouk hawthorn are produced by compressing hawthorn pulp. Besides,
candied apple produced by being dipped into hawthorn syrup or hawthorn fruit skewered by being
dipped into thickened hawthorn syrup like the formed of hawthorn sweet is sold in markets. Hawthorn
chocolate should not be forgotten. As a result; although hawthorn is naturally overgrown in our
country, the value of it is not fully understood. Producing hawthorn plant which is a functional food in
desired quality and quantity, providing continuity, evaluating the market potential is very important. In
this regard, producers, consumers and enterprises should be informed adequately and the necessary
benefits should be provided. Keywords: Hawthorn, Functional Food, Health

KEYWORDS
Hawthorn, Functional Food, Health

Corresponding Author: RABA SERPL GNHAN, rsgunhan@selcuk.edu.tr

1
SELUK NVERSTES

1268
 Poster Session 9
Submission ID: 1217

COMPARISON OF ESSENTIAL OIL COMPOSITIONS OF FRESH AND

DRIED PLANT OF SALVIA SCLAREA L. IN TURKEY

1 2 3 2 2
SLEYMAN DOU , YKSEL KAN , YAVUZ BACI , SADIYE AYE ELK , REM AYRAN

ABSTRACT
In this study, essential oil compositions of Salvia sclarea L. (dried and fresh aerial parts)
collecting from natural habitat was investigated. Essential oil was obtained by hydrodistillation for 3 h
using Clevenger type apparatus and the compositions was determined in GC-MS. In this research, it
was observed that the essential oil compositions varied with respect to be fresh or dry of the plant
parts. The LSD test results revealed that the highest essential oil components were germacrene-D
(46,62%), bicyclogermacrene (27,184%) and trans--caryophyllene (8,606%) in fresh aerial parts,
while the highest components were germacrene-D (45,593%), bicyclogermacrene (19,741%) and -
copaene (3,997%) in dried part of the plant. Furthermore, some essential oil components were not
found in the fresh aerial parts, while it was determined in dried parts of the plant. On the contrary,
some components were observed in the fresh aerial parts, while it was not found in dried parts of the
plant. The objective of the study was that the determination of the differences between the essential oil
compounds and compositions varied according to be the plant fresh or dry.

KEYWORDS
Essential oil, endemic, fresh parts, dried parts, Salvia sclarea

Corresponding Author: SLEYMAN DOU, suleymandogu@gmail.com

1
NECMETTN ERBAKAN NVERSTES AHMET KELEOLU ETM FAKLTES BYOLOJ ETM
2
SELUK NVERSTES ZRAAT FAKLTES TARLA BTKLER
3
SELUK NVERSTES FEN FAKLTES BYOLOJ BLM

1269
 Poster Session 9
Submission ID: 1218

COMPARISON OF CHEMICAL CONTENT OF WILD AND

CULTIVATED FORMS OF HYPERICUM PERFORATUM L.

1 2 3 3
SLEYMAN DOU , YAVUZ BACI , YKSEL KAN , SADIYE AYE ELK

ABSTRACT
In this study, essential oil compositions of the dried and fresh aerial parts of Hypericum
perforatum L. collected from wild and cultivated in the research field was investigated. Essential oil
was distilled by using Clevenger type apparatus for 3 h and the chemical compositions were detected
in GC-MS. The oil yields of the collected and cultivated plant materials were observed to be 0.2 ml
and 0,13; 0,1 ml and in amount tr in fresh and dried aerial parts, respectively. Drying of the material
had negative effect on the oil yield both in the collected and cultivated plant materials. Besides
essential oil yields, in this research the differences with respect to composition and components were
detected between the cultivated and collected plants. On the other hand, in this study, it was
determined that the essential oil compositions varied with respect to be fresh or dry of the plant parts.
There were 9 and 63, 36 and 73 of essential oil components were observed in the fresh and dried parts
of the collected and cultivated plant materials, respectively. It was observed that the major essential oil
components were germacrene D, n-hexadecanoic acid, trans-caryophyllene, pulegone, 12-crown-4,
2.2-dimethyl-4,4,5,5-tetramethoxybiphenyl, carvacrol and para-menth-3-en-8-ol. The aim of the
research to detect the differences between the essential oil compounds and compositions varied
according to be the plant fresh or dry and wild or cultivated.

KEYWORDS
Essential oil, Hypericum perforatum, oil yields, components

Corresponding Author: SLEYMAN DOU, suleymandogu@gmail.com

1
NECMETTN ERBAKAN NVERSTES AHMET KELEOLU ETM FAKLTES BYOLOJ ETM
2
SELUK NVERSTES FEN FAKLTES BYOLOJ BLM
3
SELUK NVERSTES ZRAAT FAKLTES TARLA BTKLER BLM

1270
 Poster Session 9
Submission ID: 1219

THERAPEUTIC USAGE OF BLACK CUMIN (NIGELLA SATIVA)

SEED AND OIL

1 2 2
DR. EMRE ZTOPRAK , DO. DR. BURAK CEM SONER , PROF. DR. AYE SADE AHN

ABSTRACT
Black cumin plant (Nigella sativa; kalonji, black caraway, schwarzkmmel) is a member of
Ranunculaceae family that grows widely in many countries, especially in Middle East, Eastern Europe
and Eastern Asia countries. However the chemical content of plants varies generally according to the
harvest season of the plant, species and the cultivated climate; black cumin plants are generally grown
in countries like Egypt, Sudan, Ethiopia, India, Turkey and Syria. There are many different species of
the plant such as Nigella sativa L, Nigella damascena and Nigella arvensis. In particular, the seeds of
Nigella sativa L. are widely used as folk medicines for traditional herbal treatment and spices. The
parts used as drog are the seeds and oil that obtained from seeds. Black cumin seed is a rich source of
carbohydrates, fats, vitamins, minerals, proteins and amino acids. Black cumin seed contains 16-20%
protein, 33-34% carbohydrate, 0.4-0.7% essential oil and over 30% fixed oil. Analysis by high
pressure liquid chromatography showed that there are 4 main active substances in Nigella sativa seed
oil. These are thymoquinone, dithymoquinone, thymohydroquinone (nigellone) and thymol.
Thymoquinone is 2-isopropyl-5-methyl-benzoquinone. Black cumin seed oil is also very rich in
unsaturated fatty acids. While the linoleic acid constitutes the main part of the unsaturated fatty acids,
the oleic acid constitutes the lesser part. Other unsaturated fatty acids are arachidonic acid and
eicosadienoic acids. It has also saturated fatty acids such as palmitic, stearic and myristic acid.
Because of the wide variety of substances in the composition of Nigella sativa and the different
therapeutic effects of each other, Nigella sativa has many different pharmacological effects. Although
more detailed informations are needed about its pharmacological effect mechanisms; black cumin seed
and oil has antitumoral, analgesic, antiinflammatory, antipyretic, antiallergic, antimicrobial,
antiasthmatic, immunomodulator, antioxidant, antidiabetic, gastroprotective, radioprotective,
hepatoprotective, nephroprotective, neuroprotective, antihypertensive, antiepileptic, lactagogue and
antihyperlipidemic effects. Nigella sativa seeds and oil have been used by ancient Egyptian and Greek
physicians to improve headache, nasal congestion, toothache, diarrhea and also as an antihelmintic and
lactagogue agent. They are used in Middle and Far East countries at traditional treatment in many
diseases such as bronchial asthma, headache, dysentery, infection, obesity, back pain, hypertension
and gastrointestinal problems. In addition their use in skin diseases such as eczema has been accepted
all over the world. In Turkey, there is also a Nigella sativa seed oil agent which licensed according to
the Ministry of Health Traditional Herbal Medicinal Product Regulation and received 3 indications.
These indications are allergic rhinitis treatment, regulation of blood lipid levels and strengthening the
immune system. Dosage in these indications are determined as 1800-3600 mg/day for adults and 40-
80 mg/kg/day for children. When its side effect profile is considered, no undesirable effects have been
reported other than nausea, vomiting and diarrhea with unknown frequency. When all these

Corresponding Author: PROF. DR. AYE SADE AHN, aysesaide@gmail.com

1
HELVACIZADE GIDA LA KMYA SAN. TC. A.
2
KONYA NECMETTN ERBAKAN NVERSTES MERAM TIP FAKLTES TIBB FARMAKOLOJ ABD

1271
informations are evaluated together, Nigella sativa seeds and oil are safe herbal agents which can be
used as an adjuvant in the treatment of many diseases.

KEYWORDS
Nigella sativa, therapeutic effects

1272
 Poster Session 9
Submission ID: 1220

SOME TEA PLANTS TRADITIONALLY USED IN ELAZI

1 2 1
UUR AKILCIOLU , RIDVAN POLAT , EBRU YCE BABACAN

ABSTRACT
This study was conducted for the purpose of determining the usage of plants used as tea by the
local community in Elaz and central villages. The study was conducted in April-September, 2015.
Villages were visited in the region throughout the study. As a result of investigations being carried out
in the region, 19 plants from 8 plant families being traditionally used were determined. The plants
were scientifically named by using the Flora of Turkey. In the study, local names, localities of plants,
as well as their parts being used as tea and other data were recorded and tabulated.

KEYWORDS
Etnobotany, tea plants, traditional use, Elaz

Corresponding Author: UUR AKILCIOLU, ucakilcioglu@yahoo.com

1
MUNZUR NVERSTES
2
BNGL NVERSTES

1273
 Poster Session 9
Submission ID: 1221

SOME SPICE PLANTS TRADITIONALLY USED IN ELAZI

1 1 2 3
UUR AKILCIOLU , EBRU YCE BABACAN , MUSA DENIZHAN ULUSAN , RIDVAN POLAT

ABSTRACT
Abstract This study was conducted for the purpose of determining the usage of plants used as
spice by the local community in Elaz and central villages. The study was conducted in April-
September, 2015. Villages were visited in the region throughout the study. As a result of investigations
being carried out in the region, 15 plants from 7 plant families being traditionally used were
determined. The plants were scientifically named by using the Flora of Turkey. In the study, local
names, localities of plants, as well as their parts being used as spice and other data were recorded and
tabulated.

KEYWORDS
Etnobotany, spice plants, traditional use, Elaz

Corresponding Author: UUR AKILCIOLU, ucakilcioglu@yahoo.com

1
MUNZUR NVERSTES
2
SLEYMAN DEMREL NVERSTES
3
BNGL NVERSTES

1274
 Poster Session 9
Submission ID: 1222

THE HERBAL DERIVED NATURAL ANTIMICROBIALS

1
AYE AKAN

ABSTRACT
In this study, the different studies about the components that has obtained from extracts of
herbal and spices and essantial oils which has antimicrobial activities, the antimicrobial activities in
food borne pathogens, the experiments to extend shelf lifes of food, has been researched. The food
consumers started to show interest to natural compounds, so that the studies about natural compounds
to increase shelf life anf quality of food has been increased. The herbal and spices has antimicrobial
activities with the components as phenolic acids, flavonoids, tannins etc. However, there can be
different results when the studies was done on food. The antimicrobial activities of natural compounds
could be increased, if several antimicrobial components are used together or by evaluating the factors
likewise pH of food, storage conditions, process of production.

KEYWORDS
Herbal, Essantial oil, Antimicrobial acitivity

Corresponding Author: AYE AKAN, ayseakan86@gmail.com

1
YILDIZ TEKNK NVERSTES

1275
 Poster Session 9
Submission ID: 1223

THE ROLE OF SPICES IN THE TREATMENT OF HELICOBACTER

PYLORI

1 2 3 3
MEHMET KEMAL , SEVGI KOLAYLI , ENGL ALPAY KARAOLU , NIMET BALTA

ABSTRACT
Helicobacter pylori, a gram negative pathogen bacterium, causes gastritis, reflux, ulceration in
the stomach. This bacterium that colonizes in one out of every two individuals is quite resistant to
antibiotics. Urease enzyme released by H. pylori converts urea into ammonia; thus, this bacterium
survives in stomach inner wall thanks to formed ammonia which makes an alkaline environment.
Therefore, inhibition effects of the bacterial urease are defined as an anti-bacterial activity. In this
study was to be researched inhibition effect on the bacterial urease enzyme of some spices commonly
used in human diet. For this purpose, inhibitory effects on the Helicobacter pylori urease of ethanolic
extracts of spices such as thyme, clove, isot pepper, turmeric, mint, black sesame and cinnamon
purchased from herbalist were investigated as a screening study. Enzyme inhibition was calculated
with five different sample concentrations. It was found that all samples displayed inhibition effect at
varying concentration on this enzyme and values of IC50, defined as sample amount that reduces
enzyme activity by 50%, ranged from 0.96 (mg/ml) to 5.05 (mg/ml). As a result, the highest activity
from thyme and isot pepper samples were ascertained. Therefore, our studies proceed about
antibacterial activity with thyme and isot pepper extracts.

KEYWORDS
Helicobacter pylori, urease, spices, antibacterial

Corresponding Author: MEHMET KEMAL, mhkemal@gmail.com

1
ARTVN ORUH NVERSTES
2
KARADENZ TEKNK NVERSTES
3
RECEP TAYYP ERDOAN NVERSTES

1276
 Poster Session 9
Submission ID: 1225

THE USE OF PHYTOTHERAPY AND ALTERNATIVE MEDICINE BY

CANCER PATIENTS OUTPATIENT CHEMOTHERAPY UNIT IN
USAK UNIVERSITY MEDICINE SCHOOL EDUCATION AND
RESEARCH HOSPITAL

1 2
AYE ZDEMIR , DUDU BAYRAKTAR DNDER

D
TE
ABSTRACT
With hundred of thousands of plant species on earth, We are bestowed with an glorious
richness of medicinal remedies from Earth- Mother. The Herbal Medicine (Phytotherapy) and
Alternative Medicine have been most commonly used to treat diseases thousands of years in our

EN
country and in the world. The interaction of herbal product and cancer drugs are not exactly known.
The treatment of cancer is an important treatment that needs to be interrupted. Most poeple especially
cancer patients use herbal products as a cancer treatment. Complementary and Alternative Medicine in
cancer patients in this study was conducted to examine the methods of use 100 cases at Outpatient
Chemotherapy Unit in Usak University Medical School Education and Research Hospital(1 January
ES
2017- 25 March 2017). Methodology: The the sample of the research of planned at identifier type
consisted of 100 cancer patients treated at Outpatient Chemotherapy Unit in Usak University Medical
School Education and Research Hospital (January 2017- March 2017). During the research process
was used questionnaire personal information form and alternative treatment. Results: Only 23 of the
PR

100 patients in the sample uses alternative treatment methods. While 20 patients use herbal treatment,
3 patients use acupuncture. 13 of the 23 patients is women, and 10 of their is male.77 patients are not
using any alternative treatment methods . Most of the patients who use alternative treatment are
women and have lung and breast cancer. Today reactions against the use of synthetic drugs due to
their severe side effects raised the interest in herbal products. Although many cancer types can be
almost fully cured in early stages with cancer treatment in modern medicine, especially
T

complementary therapy plays an important role in cancer treatment.

O

KEYWORDS
N

Cancer, Phytotherapy, Alternative Medicine, Complementary Medicine

Corresponding Author: AYE ZDEMR, ayse.ozdemir@usak.edu.tr

1
UAK NVERSTES TIP FAKLTES / TIBB BYOKMYA ABD
2
UAK NVERSTES SALIK YKSEKOKULU/ HEMRELK

1277
 Poster Session 9
Submission ID: 1227

DETERMINATION OF ESSENTIAL OIL COMPOSITION AND

INVESTIGATION OF THE PHENOLIC SUBSTANCE AND
ANTIOXIDANT EFFECT OF HELICHRYSUM STOECHAS FROM
HATAY

1 1
MAHIR TMUR , SMAIL AKA

ABSTRACT
DETERMINATION OF ESSENTIAL OIL COMPOSITION AND INVESTIGATION OF
THE PHENOLIC SUBSTANCE AND ANTIOXIDANT EFFECT OF HELICHRYSUM
STOECHAS FROM HATAY Mahir Timur*, smail Aka Mustafa Kemal University, Altnz
Vocational School, Department of Food Process, Hatay, Turkey *E-mail: mahirtimur@gmail.com
People traditionally benefit from medical herbs to protect themselves from diseases. Antioxidants are
the protective or inhibiting compounds that help to heal in medical plants. Antioxidants can prevent or
reduce oxidative damage even at low concentrations(1). It has been pointed out that compounds such
as polyphenolic substances, vitamins and enzymes, which are found in the structure of plants
consumed as food, are directly related to the antioxidant effects of plants. This antioxidant effect can
significantly prevent the damages caused by reactive oxygen species and reactive nitrogen species
which cause oxidative stress. (2,3). Helichrysum Stoechas is an expectorant and is used in the
treatment of colds. [4] In this study, it was aimed to determine the essential oil composition, phenolic
substance and antioxidant activity of Helichrysum Stoechas, locally known as kudama, growing in the
region of HATAY Antakya Castle. Helichrysum Stoechas' essential oil was obtained by using
Clevenger apparatus and found to be 0.3% on dry matter. The essential oil composition was
determined by gas chromatography and the essential oil was found to be D-FENCHONE 0.77%,
Caryophyllene 1.10%, 2-Naphthalenemethanol 2,3,4,4a, 5, 4.89%, benzene 1,2,3,4-tetramethoxy--5-
(2-propenyl) %34.69, Benzene, 1,2,3-trimethoxy-5-(2-propenyl) % 3.24 Myristicin % 23.35, Lauric
acid % 1.17, Apiol %27.38, Myristic acid % 1.38. Methanol extract was obtained for phenolic
substance and antioxidant capacity assay. The portion of the plant dissolved in methanol was found to
be 15.3% on dry matter. The total phenolic substance concentration was determined according to the
Folin-Ciocalteu method and the total amount of phenolic substance in the methanol extract was
calculated as 256 mg / g gallic acid equivalent (GAE). The free radical scavenging effect was
determined according to the DPPH method and the results were compared with BHA and BHT. Plant
extract; 94.28%, 94.49%, 92.16%, 67.80%, 34.11%, BHA; 91.13%, 90.67%, 89.61%, 86.01%,
78.60%, BHT; , 91.95%, 89.23%, 68.64%, 53.39% and 29.23% inhibition values, respectively. The
DPPH removal activity of the standards and plant extract was found to decrease as plant extract>
BHA> BHT. The effective antioxidant concentration EC50 values required for the 50% inhibition of
the DPPH radical in the reaction medium were found to be BHA: 0.022, plant: 0.038 and BHT: 0.057
(g / mL), respectively. The low EC50 value is indicative of the high radical scavenging activity. Total
antioxidant activity was determined by beta-carotene linoleic acid method. Antioxidant activity values

Corresponding Author: MAHR TMUR, mahirtimur@gmail.com

1
HATAY MUSTAFA KEMAL NVERSTES

1278
were calculated as BHT: 73.33%, plant: 68.75% and BHA: 63.15%. Reductive Antioxidant Capacity
was made according to CUPRAC Method (Gl et al., 2006). from the Trolox calibration curve
CUPRAC activity TEACCUPRAC:71,65 g / mL was found. It is obvious that the obtained result
shows that Helichrysum stoechas has an effect close to the synthetic antioxidants BHT (Butylated
Hydroxytoluene) and BHA (Butylated Hydroxanisole). Nowadays, as the tendency of natural
treatment methods increases, the composition of medicinal and aromatic plants should be determined
and their use efficiency will be increased according to active compounds. References: 1- VAYA J and
AVRAM M. 2001, Curr. Med. Chem. Immun.,Endoc. & Metab. Agents, 1, 99-117. 2-SOONG YY,
BARLOW PJ, 2004. Food Chemistry 88, 411417 3-NAWAZ H, SH J, MTTAL GS, KAKUDA Y,
2006, Separation and Purification Technology 48: 176181, 4-Grieve A., 1984, Modern Herbal.
Penguin ISBN 0-14-046-440-9 Not so modern (1930's) but lots of information, mainly temperate
plants. 5-Gl, K., Altun, M., zyrek, M., Karademir, S. E. and Apak, R. 2006. Food Science and
Technology, 41: 76-85.

KEYWORDS
medicinal and aromatic plants, essential oil, phenolic compounds, antioxidant effect

1279
 Poster Session 9
Submission ID: 1229

NOVEL METHODS FOR QUALITY ANALYSIS OF ESSENTIAL OILS

PRODUCED AS FOOD SUPPLEMENT

1 2
KADIR GRBZ GNER , H. MURAT VELIOLU

ABSTRACT
Recent trends of natural diet and curing have provoked the use of herbal products and food
supplements in the society. Therefore, essential oils that are produced and consumed as food
supplement are of great interest to the consumers. Turkey has a great biodiversity in the meaning of
aromatic plant cultivation and many of essential oil plants grow naturally. The use of essential oils in
food, medicine and perfume industries has been increasing in recent years. In this context, the number
of exported aromatic plant species has reached to almost 100 in Turkey which yield an income of USD
100 million annually. Nowadays, consumers are increasingly concerned about the quality and safety of
food products. Hence, the origin and health effects of food constituents are being discussed in public
media. Essential oils are more expensive than the other vegetable oils and that is why the
determination of fraudulent malpractices is important in these products. The addition of cheaper oil in
expensive one is very well known adulteration for essential oils which affects not only the consumers
health but also causes an unfair profit. There is a need for rapid and economic analysis methods for
determination of essential oil quality. Gas chromatography / mass spectroscopy (GC/MS) is still
remain as a golden method. However, chromatographic methods are time consuming, costly and they
need a specialist for the analysis which make them disadvantageous compared with
spectrophotometric methods. Raman, Fourier transform infrared reflectance (FTIR) and near infrared
reflectance (NIR) spectroscopies have been gaining great interest during the recent years. As rapid and
sensitive techniques, spectroscopic methods can give biochemical finger prints of the material.
Additionally, these methods are suitable for applications which require green chemistry limitations. In
the present paper, the novel spectroscopic methods in determination of quality of essential oils were
summarized and exemplified using literature studies.

KEYWORDS
Essential oil, quality, spectroscopy

Corresponding Author: KADR GRBZ GNER, kguner@nku.edu.tr

1
NAMIK KEMAL UNVERSTY, DEPARTMENT OF FOOD ENGNEERNG
2
NAMIK KEMAL UNVERSTY

1280
 Poster Session 9
Submission ID: 1231

A DIFFERENT PERSPECTIVE ON SAGE (SALVIA SP. ): USAGE IN

LANDSCAPE ARCHITECTURE

1 1
MERT AKIR , RN DNMEZ

ABSTRACT
Turkey, with rich flora, reserves lots of medicinal and aromatic plants within its structure. The
purpose of the plants with their therapeutic purpose is as old as history of humanity. Despite
significant developments in medicine, today, the traditional usages of medicinal herbs are very
important and are accepted as a scientific discipline. In the past centuries, medical and aromatic plants
have been used in traditional house gardens for both ornamental and utility purposes. Today,
medicinal and aromatic plants take place in parks and thematic garden designs and are very
interesting. It is known that sage, which is widely used as a medicinal and aromatic plant, is close to
100 species naturally distributed in our country. Especially, because of their nice smells and flowers,
they can be suitable for use in landscape areas. However, except for very special gardens, these plants
are rarely used. Within the scope of this study, sage species which spread naturally in Turkey will be
evaluated in terms of shape, form and flower characteristics and their use in plant design has been
determined.

KEYWORDS
Sage, plant design, landscape architecture

Corresponding Author: MERT AKIR, pmmertcakir@gmail.com

1
SLEYMAN DEMREL NVERSTES

1281
 Poster Session 9
Submission ID: 1233

SCREENING OF ANTIOXIDANT, ACETYL- AND BUTYRYL-

CHOLINESTERASE INHIBITORY ACTIVITIES OF ENDEMIC
BUNIUM CRASSIFOLIUM BATT. GROWING IN ALGERIA

1 2 2 1 1 2
NABILA SOUILAH , ZAIN ULLAH , KUBRA ELK , KAMEL MADJROUBI , SALAH AKKAL , MEHMET ZTRK

ABSTRACT
Screening of Antioxidant, Acetyl- and Butyryl-Cholinesterase Inhibitory Activities of
Endemic Bunium crassifolium Batt. Growing in Algeria Nabila SOUILAH1, Zain ULLAH2, Kubra
ELK2, Kamel MADJROUBI1, Salah AKKAL1 and Mehmet ZTRK2 1Department of Chemistry,
Faculty of Sciences, University of Constantine 01, 25000, Constantine, Algeria 2Department of
Chemistry, Faculty of Sciences, Mula Sitki Koman University, Ktekli-48000, Mula, Turkey
zainullah@posta.mu.edu.tr The Bunium genus contains aromatic and medicinal plants such as Bunium
crassifolium Batt. The Bunium genus contains aromatic and medicinal plants such as Bunium
crassifolium Batt. This study evaluates the antioxidant and anticholinesterase activities of Bunium
crassifolium, which is an extremely rare endemic species of the Bunium genus. The plant sample was
collected at full flowering stage from Srradi (Annaba), in the north eastern of Algeria in May 2015.
The aerial part of the plant was dried under shadow and grinded (65 g), afterwards macerated in
methanol (100 %) at constant stirring rate (200 rpm) for 24 hours, then filtered by Whatmn N01 filter
paper and evaporated the solvents with rotary vacuum evaporator and named as B.C-100. For a 2nd
extraction the grinded dried aerial parts of the plant (10 g), was macerated in boiling methanol and
distilled water (70:30) followed by the same procedure and named as B.C-70. DPPH, ABTS, -
carotene-lineloic acid, acetyle- and butyryl cholinesterase activities were used to evaluate the
antioxidant and enzymes inhibitory potential of the obtained extracts. The B.C-70 extract showed
more potential against ABTS, -carotene and butyryl choline esterase with an IC50; 12.69 3.78,
68.15 1.02 and 19.41 0.41 g/mL respectively. Against DPPH and acetyl cholinesterase BC-100
was more potential with an IC50: 49.83 2.37 and 20.15 2.71 g/mL, respectively. Since Bunium
crassifolium exhibited significant antioxidant and anticholinesterase activities, further studies by
activity guided fractionation are necessary. Bunium crassifolium containing antioxidant and
anticholinesterase compounds can be used in pharmaceutical and food industries. Acknowledgements:
Authors are thankful to TUBITAK for financial supporting of Graduate Scholarship Programme for
International Students TUBITAK-BIDEB-2215. Authors also thankful to the Chemistry departments
of Constantine University and Mugla Sitki Kocman University for providing lab facilities. Keywords:
Bunium crassifolium, Antioxidant activity, Anticholinesterase activity References ztrk M, Tel G,
Aydomu-ztrk F, Duru ME (2014). The cooking effect on two edible mushrooms in Anatolia:
Fatty acid composition, total bioactive compounds, antioxidant and anticholinesterase activities. Rec.
Nat. Prod 8 (2), 189-194. Quezel P, et Santa S (1963). Nouvelle Flore de lAlgrie et des Rgions
Dsertiques Mridionales. Edition du Centre National de la Recherche Scientifique Paris 7, Tome II.

Corresponding Author: ZAN ULLAH, zainullah@posta.mu.edu.tr

1
DEPARTMENT OF CHEMSTRY, FACULTY OF EXACTE SCENCES, UNVERSTY OF CONSTANTNE 01, 25000
2
DEPARTMENT OF CHEMSTRY, FACULTY OF SCENCES, MULA STK KOMAN UNVERSTY, KTEKL-
48000, MULA

1282
P: 1170. Vasilava MG, Klingkov EV, Pimenov MG (1985). Karyotaxonomic analysis of genus
Bunium. Plant Systematics and Evol 149, 71- 88.

KEYWORDS
Bunium crassifolium, Antioxidant activity, Anticholinesterase activity

1283
 Poster Session 9
Submission ID: 1234

EFFECT OF THE ANTIOXIDANT PROPERTY OF EXTRACT

OBTAINED FROM PAPAVER SOMNIFERUM LEAF

1 1 1 1 2
ELMAS ULUTAS , AZIZ BLBL , TUBA BLBL , MUSTAFA EVCMEN , MER YALINKILIC

ABSTRACT
In this study, the antioxidant efficiency of poppy plant (papaver somniferum) were analysed
with different methods. For this purpose, the extractions of the papaver plant that reached to 10-15 cm
of length and was ground by drying were carried out by using ethanol, methanol and ethyl acetate
solvents. Afterwards, With Folin-Ciocalteu reagent the total phenolic compound, and with 1,1-
Diphenyl -2- picrylhydrazyl (DPPH) free radical eliminating activity, superoxide radical eliminating
activity and Beta-carotene bleaching method the antioxidant properties were identified. The results
obtained were evaluated by comparing with ascorbic acid, butyl hydroxytoluene (BHT) and butylated
hydroxyanisole (BHA) standard substances. It was specified that as gallic acid equivalent, the total
phenolic substance amount changes between the ranges of 63,4 82,5 and is found to be maximum in
the ethanol extract. It was seen that all the extracts show super oxide and DPPH radical elimination
efficiency and at the same time the maximum efficiency is in the ethanol extract. Similarly, in the
determination of antioxidant activity that was realized by using -Carotene bleaching method, it was
determined that all the extracts show antioxidant efficiency. As a result it was understood that
although the papaver somniferum extracts are weak in terms of phenolic substance content, they show
antioxidant efficiency and ethanol is the most suitable solvent for antioxidant efficiency

KEYWORDS
Papaver somniferum, poppy, antioxidant activity, phenolic substance

Corresponding Author: ELMAS ULUTAS, eulutas@aku.edu.tr

1
AFYON KOCATEPE NVERSTES, VETERNER FAKULTES
2
MEB, AFYON FEN LSES

1284
 Poster Session 9
Submission ID: 1238

DETERMINATION OF THE ANTIOXIDANT EFFICIENCY OF

MYRTLE (MYRTUS COMMUNIS L.) ESSENTIAL OIL

1 2 2
ELMAS ULUTAS , AZIZ BLBL , TUBA BLBL

ABSTRACT
In this study, it was aimed to identify the phenolic substance content and antioxidant property
of Myrtle (Myrtus communis L.) essential oil. With Folin-Ciocalteu reagent the total phenolic
substance content and with 1,1- Diphenyl -2- picrylhydrazyl (DPPH) free radical elimination,
superoxide radical elimination activities and beta-carotene bleaching method, the antioxidant
properties were identified. The results obtained were evaluated by comparing with ascorbic acid, butyl
hydroxytoluene (BHT) and butylated hydroxyanisole (BHA) standard substances. The density of
myrtle essential oil obtained by hydro distillation method was specified as 0.897 g/ml. It was found
that as gallic acid equivalent, the total phenolic substance amounts in the oil is 128,12 g/ml; and by
DPPH free radical and superoxide radical elimination activities the antioxidant efficiency was
respectively found as 33,8% ; 43,4% and 38,9%. In conclusion, while there is phenolic substance at a
moderate level in the myrtle essential oil (Myrtus communis L.), it was also identified that it has
strong antioxidant efficiency.

KEYWORDS
Mersin, (Myrtus communis L.), antioksidan etkinlik, fenolik madde

Corresponding Author: ELMAS ULUTAS, eulutas@aku.edu.tr

1
AFYON KOCATEPE NVERSTES, VETERNER FAKULTES
2
MERSN, (MYRTUS COMMUNS L.), ANTOKSDAN ETKNLK, FENOLK MADDE

1285
 Poster Session 9
Submission ID: 1240

MUTAGENIC AND ANTIMUTAGENIC POTENTIAL OF SOME

COMMERCIAL PHENOLIC COMPOUNDS (CATHECHIN,
EPICATHECHIN, CAFFEIC ACID)

1 1 1 1
AHMET UYSAL , GKHAN ZENGIN , ERDOAN GNE , MEHMET AKZ

ABSTRACT
There are approximately 300,000 documented species of higher plants on the planet, which
synthesize an enormous number of chemicals of diverse structure and class (more than 200,000
isolated and identified individual chemical entities). These compounds can be further divided into
primary and secondary metabolites. Secondary metabolites are structurally and chemically much more
diverse than the primary metabolites and refer to compounds present in specialized cells that are not
directly essential for basic photosynthetic or respiratory metabolism but are thought to be required for
plants survival in the environment. Secondary metabolites apparently act as defense (against
herbivores, microbes, viruses, or competing plants) and signal compounds (to attract pollinating or
seed dispersing animals), as well as protecting the plant from ultraviolet radiation and oxidants. In this
study, catechin, epicatechin and caffeic acid obtained commercially and found in plants were
evaluated for their potential of mutagenic and antimutagenic activities by Salmonella/microsome test
system both in the presence and absence of metabolic activation enzymes (S9 mix). Chemicals were
tested for their toxicity before and non-toxic doses (5000, 1000 and 500 g/plate) of them were used in
the test. Salmonella typhimurium TA98 and TA100 strains did not increase in the number of revertant
colonies compared to the negative control when the bacteria were treated with three phenolic
chemicals. In other words, catechin, epicatechin and caffeic acid were not found to be mutagenic for
TA98 and TA100 strains in Ames test. Because of the compounds were not mutagenic,
antimutagenicity of phenolics were determined. It was determined that catechin and epicatechin
revealed strong antimutagenicty against 4-nitrophenylendiamine in the absence of S9 mix for TA98 at
doses of 5000, 1000 and 500g/plate (for catechin 69%, 73%, 49% inhibition; for epicatechin 68%,
56%, 53% inhibition, respectively). Caffeic acid manifested moderate antimutagenicity with rates of
36% and 33% inhibition at doses of 5000 and 1000 g/plate. By the addition of S9 mix antimutagenic
actions of the compounds decreased and showed weak antimutagenicity against 2-aminoflourene
except for 5000g/plate dose of catechin and epicatechin for TA98. Only epicatechin exhibited strong
antimutagenicity against sodium azide with a rate of 46% at a concentration of 5000g/plate for
TA100 without S9 mix. The strongest antimutagenicity (88%) was revealed by catechin in the
presence of metabolic activation enzymes for TA100 at a dose of 5000g/plate. Also epicatechin and
caffeic acid manifested strong antimutagenicity against 2-amino anthracene at the same dose with rates
of 67% and 46% inhibiton, respectively. It was concluded that catechin, epicatechin and caffeic acid,
plant secondary metabolites, have moderate to strong antimutagenic action against well-known
mutagens. Plant including this phenolics may be the natural source of antimutagens and used in
pharmaceutical industry.

Corresponding Author: AHMET UYSAL, ahuysal@selcuk.edu.tr

1
SELUK NVERSTES

1286
KEYWORDS
Phenolics, antimutagenicity, epicatechin, catechin, caffeic acid

1287
 Poster Session 9
Submission ID: 1241

EFFECTS OF PUNICA GRANATUM PEEL POWDER ON HUMORAL

IMMUNITY OF LAYER HENS*

1 2 1 1
UKUN SAIT UAN , TAHIR BALEV , ZAFER SAYIN , ASLI SAKMANOLU

ABSTRACT
Effects of Punica Granatum (Pomegranate) peel added to layers rations with 1.5 % ratio on
humoral immunity were investigated. A number of 24 Hy-line layer were used. Trial was caried out in
Hmeyra zgen Research and Application Farm, Veterinary Faculty of Seluk. Some amount of fresh
Punica Granatum was supplied by local market, peeled, dried in oven and then powdered by means of
grinder. Except one of the control, the others were fed on ratios supplemented with 1.5% powder of
Punica Granatum peel. The vaccine groups were administered by La Sota strain through drinking
water at the beginning of the study against New Castle Diseases (ND) virus. Humoral immune
response to ND virus were measured at d 30 and d 60 from serum samples by Hemagglutination
Inhibition (HI) assay. In the trial, supplementation of ration with 1.5% powder of Punica Granatum
peel suppressed the specific humoral response against ND virus (P<0.01). To detect effects of this
supplement in lower quantities are subject of future work . *The results discussed by this presentation
is a part of the research (15401148) supported by BAP (Seluk niversitesi).

KEYWORDS
Laying hens, Punica granatum, humoral immunity

Corresponding Author: UKUN SAT UAN, usucan@selcuk.edu.tr

1
SELUK NVERSTES, VETERNER FAKLTES, MKROBYOLOJ AD, KONYA.
2
SELUK NVERSTES, VETERNER FAKLTES, HAYVAN BESLEME AD, 42250, KONYA,

1288
 Poster Session 9
Submission ID: 1242

THE EFFECT OF CAPSAICIN ON HEALTH

1 1 1
PEK KARADUMAN , RECEP PALAMUTOLU , CEMAL KASNAK

ABSTRACT
Red pepper bitter (Capsicum annuum) is cultivated for 7000 years in various parts of the
world such as South Asian countries and South-East Anatolia region of South America, and belongs to
the Solanaceae family and it is used as seasoning and sauce in foods due to its sharp and bitter flavor.
Red Pepper has been widely used in the treatment of various diseases for a long time and used since
ancient times to give flavor to food. Bitterness of capsicum fruits are due to their capsaicin content.
Red pepper contains essential oil, fixed oil and pigments while fruits vitamin C content is rich in
green. Pepper is also an important vegetable in terms of human health. It has been reported that
capsaicin alkaloid is an important antioxidant against cancer diseases which gives bitter and spicy taste
in hot pepper. It is stated that the hot pepper is good for digestion, appetizing, muscle pain and
rheumatism, nervous system, red eye disease and seasickness. These effects are generally thought to
originate from bitterness components. Briefly, it is investigated that the capsaicin has
anticancerogenic, antilipemic, antioxidant, energy balance, anti-dyspeptic, anti-bacterial,
immunsupressive effect. The purpose of this work is to compile studies on these properties of the
capsaicin.

KEYWORDS
Anti-carcinogenic, antioxidant, capsaicin, Capsicum annuum, pepper

Corresponding Author: RECEP PALAMUTOLU, rpalamutoglu@aku.edu.tr

1
AFYON KOCATEPE NVERSTES

1289
 Poster Session 9
Submission ID: 1243

APHID (HEMIPTERA: APHIDIDAE) SPECIES DETERMINED ON

SOME MEDICINAL AND AROMATIC PLANTS WITH NEW HOST
PLANTS FOR TURKEY

1 2
N. ZLAL ELEKCOLU , IIL ZDEMR

ABSTRACT
The aphids are fed by sucking the sap of host plants. Due to loss of juice, they cause wilting
and drying out of young shoots and leaves. By preventing fruit growth, important crop losses occurs.
They cause the spread of various plant diseases by carrying and infecting viruses. This study was
carried out in 2015-2016 to determine the aphid species found on some medicinal and aromatic plants
in Adana (Saram, Karaisal provinces). In the study, 26 plant species were examined and a total of
24 aphid species belonging to 4 subfamily and 18 genus were identified. Cynara scolymus L., Mentha
piperita L., Achillea asplenifolia L., Linum usitatissimum L., Thymus serpyllum L., Origanum
majorana L., Perovskia atriplicifolia Benth., Dracocephalum moldavica L., Melissa officinalis L.,
Salvia officinalis L., Salvia fruticosa Mill., Origanum onites L., Origanum vulgare L., Matricaria
chamomilla L., Coriandrum sativum L., Calendula officinalis L., Digitalis purpurea L., Origanum
syriacum var. bevanii, Echinacea purpurea (L.), Rosa damascena Miller, Kitaibelia balansae Boiss.,
Pelargonium graveolens L., Artemisia annua L. and Trigonella foenum-graecum L. are determined as
the new hosts in Turkey for the aphid species which they have been detected on.

KEYWORDS
Medicinal plant, aromatic plant, aphid, new host

Corresponding Author: N. ZLAL ELEKCOLU, nelekcioglu@cu.edu.tr

1
UKUROVA NVERSTES, KARASALI MESLEK YKSEKOKULU
2
ZRA MCADELE MERKEZ ARATIRMA ENSTTS MDRL

1290
 Poster Session 9
Submission ID: 1244

CHERRY LAUREL

1 1 1
SMEYYE MEK , RECEP PALAMUTOLU , CEMAL KASNAK

ABSTRACT
Free radicals cause serious problems such as cancer, cardiovascular diseases, Alzheimer's
disease, aging. Antioxidants react with free radicals, making them ineffective and preventing them
from harming the cells. Thus, deterioration of cell structure and cancer formation risk reduces. As they
reduce cell destruction, they delay aging and provide a healthy lifestyle. While antioxidants neutralize
free radicals, there is a constant need for antioxidants in the body because they will be oxidized. Some
of the need can be met by dietary nutrients. Antioxidants can be obtained either synthetically from
outside or naturally from fruits and vegetables. Especially the amount of antioxidants in fruits and
vegetables that are consumed raw is quite high. With the increasing importance of healthy nutrition,
there is an important trend in our country for herbal medicines and organic fruit and vegetable market.
The different species of cherry laurel (Laurocerasus officinalis) that grows widely in our country in the
east of the Black Sea, Toros, North and East Marmara are grown in different parts of the world. Cherry
laurel leaf, fruit and kernel are used in different forms. In particular, the fruit is consumed raw, but
also the dried, salted forms, marmelade, jam and pickles are often consumed by local people. Fruit is
used among people because of eczema, stomach ulcer, treatment of digestive system problems. In this
study, it is aimed to compile the health effects of the cherry laurel fruit.

KEYWORDS
Anticarcinogenic, antioxidant, cherry laurel, health

Corresponding Author: RECEP PALAMUTOLU, rpalamutoglu@aku.edu.tr

1
AFYON KOCATEPE NVERSTES

1291
 Poster Session 9
Submission ID: 1246

CAPSAICIN AND ITS EFFECTS ON APPETITE

1 1 1 1
MERVE PEHLVAN , NILFER ACAR TEK , GAMZE AKBULUT , DUYGU AAGNDZ

ABSTRACT
Capsaicin, which gives chili peppers their hot and pungent taste, is an alkaloid found in the
genus Capsicum. Capsaicin is a naturally occurring phytochemical, which is an odorless, white crystal
with harsh flaring pungency. Even a solution with 1 in 17,000,000 concentration causes pain in
humans. Its effects on the human body have been studied for over a century. This compilation aims to
study the effects of capsaicin on appetite. It is widely accepted that the main mechanism of action for
capsaicin is through the activation of the TRPV1 receptor (Transient receptor potential vanilloid
receptor-1) on sensory afferents. Transient receptor potential vanilloid receptor-1 stimulation in
intestinal enteroendocrine cells with capsaicin causes the calcium dependent secretion of glucagon like
peptide 1 (GLP-1), which facilitates sensation of fullness. Although the exact mechanism is still not
clear, it is reported that capsaicin affects appetite by increasing the concentration of anorexigenic
hormone GLP-1and decreasing the concentration of orexigenic hormone ghrelin. In a study by Smeet
et al. on 30 individuals in the Netherlands, the subjects were randomly divided into two groups. One
group (CAPS) received capsaicin (1.03 g red pepper; 80 000 SHU) and the other was the control
group. While lunch content for both groups was the same (60% of energy from carbohydrates, 10%
from proteins and 30% from fat), the CAPS group also received a capsaicin tablet with their lunch.
Fifteen minutes after lunch, GLP-1 was significantly higher (p<0.05) in the CAPS group, while
ghrelin showed a tendency to decrease (p=0.07). In another study by Smeet et al. on 24 individuals,
daily consumption of 2 red peppers containing 1030 mg of capsaicin increased sensation of fullness. In
a study by Janssens et al. on 15 individuals, subjects consumed 1,03 g of red pepper (2,56 mg
capsaicin, 39.050 SHU) at each meal. These subjects consumed 7,68 mg/day of capsaicin in total over
three meals. Capsaicin consumption was shown to increase sensation of fullness and to inhibit the urge
to overeat. In summary; although the daily dosage and method of consumption of red pepper and
capsaicin differ in studies, it is evident that red pepper and capsaicin consumption suppresses appetite
and increases sensation of fullness. However, due to the variations mentioned above, daily dosage of
red pepper and capsaicin required to affect appetite is not exactly clear. Further experimental study is
required to precisely declare the effects and dosage of red pepper and capsaicin.

KEYWORDS
Capsaicin, TRPV1, appetite, fullness, GLP-1

Corresponding Author: MERVE PEHLVAN, pehlivan.merve@hotmail.com

1
GAZ NVERSTES

1292
 Poster Session 9
Submission ID: 1247

NATURAL SWEETENER STEVA

1 1 1
BEYZA MORAL , RECEP PALAMUTOLU , CEMAL KASNAK

ABSTRACT
One of the most fundamental objectives of the food industry is to guide the work aimed to the
changing consumer needs. In recent years consumers have turned to sweeteners because of the
expression that the negative effects of sugar on health in our country and in the world. Today, there are
many sweeteners that are used instead of sugar. Stevia rebaudiana is a natural sweetener, belongs to
the family of the Asteraceae (Compositae) and the motherland of the plant is Paraguay, and the
Amami mountains on the border of Brazil. The plant used in the treatment of diabetes for a long time
in South America. It has been reported that the plant contains diterpens which are the secondary
metabolites that give the sweet taste. The main sweetening compounds are stevioside and
Rebaudioside A. Other sweet compounds are present in Stevia at low concentrations. It has been found
that stevia is more sweet 250-300 times than sucrose, and dont gives metallic taste in the mouth.
Different products produced for consumers such as powder, liquid and dried forms of Stevia
rebaudiana.Studies showed that extracts fronm dry stevia leaves contain flavonoids, alkaloids, water
soluble chlorophyll and xanthophylls, hydroxycinnamic acid (caffeic, chlorogenic, etc.), neutral water
soluble oligosaccharides, free sugars, amino acids, lipids, essential oils and trace elements (aluminum,
iron, zinc, etc.). It is believed that Stevia has positive effects on diabetes, high blood pressure,
constipation, depression and nervous disorders, and the stomach and intestinal flora preserve the acid
alkali balance. It does not contain calories so its use has been the mainstay of dietary treatment in
recent times. It has been reported that Stevia leaves used in the treatment of cardiovascular diseases, as
well as plaque relief, anti-caries, pain relief. It has been stated that it is used as a garnet for the
antibacterial effect by the people, participates in the combination of toothpastes, is used in eczema and
acne treatment, is a calcium antagonist and has a positive effect on the nervous system. The aim of this
study is to compile studies on the health effects of Stevia plant.

KEYWORDS
Stevia rebaudiana, sweetener, natural, diabetes

Corresponding Author: RECEP PALAMUTOLU, rpalamutoglu@aku.edu.tr

1
AFYON KOCATEPE NVERSTES

1293
 Poster Session 9
Submission ID: 1248

HERBAL THERAPIES IN HEMODIALYSIS PATIENS

1 1 1
FERAY GENER , HILAL YILDIRAN , MAKBULE GEZMEN-KARADA

ABSTRACT
End stage renal disease is characterized by gradual deterioration of kidney function and is
often associated with a wide range of complications, such as hypertension, anemia, malnutrition, and
decreased quality of life. Hemodialysis is the primary treatment for patients with end stage renal
disease, designed to replace kidney function, and it is the most extensively used technique worldwide.
The increasing incidence of chronic kidney disease demands more effective treatment approaches.
Herbal therapies, are extremely popular worldwide and have been important among hemodialysis
patients from medical, sociological and economic perspectives. Herbal therapies may provide new
therapeutic opportunities for hemodialysis patients with the target of enhancing quality of life and
improving symptoms. There is increasing evidence that shows the potential benefits of these products
for patients with chronic kidney disease. Some studies have shown the beneficial potential of these
products in hemodialysis patients suffering complications such as muscle cramps, pruritus, and
inflammation. It is considered that herbal therapies are an option to decrease health problems of
hemodialysis patients, such as gastrointestinal, psychiatric and neurologic semptoms. Turmeric, garlic,
linden, asparagus, parsley and stinging nettle are mainly used herbal products because of
antiinflammatory, antioxidant and diuretic effects. Astragalus is another plant that is used in many
countries and has been used for centuries in traditional Chinese medicine in combination with other
herbs. Astragalus has been used as a dietary supplement for many conditions, including for diarrhea,
fatigue, anorexia, upper respiratory infections, heart disease, hepatitis, fibromyalgia, and cancer. There
are no high quality studies in people of astragalus for any health conditions. It suggested that
astragalus may help infections for patients with health problems related to kidney damage are
susceptible to infections. Pomegranate and pomegranate juice are another products used in this patient
group because it is thought to improve inflammation. The use of herbal medicine in hemodialysis
patients seems to have more risk susceptibility in comparison with that in the general population. It is
mainly due to their loss of kidney function as the most important toxin excretion system, leading to
accumulation of toxic material of herbal remedies. Volume overload and hyperkalemia are the other
important considerations of herbal therapies in these patients. Oral herbal medication has certain
limitations, such as the occurrence of side effects and potential herb-drug interactions. Multiple
treatment approach incorporating herbal medicine may offer more benefit to patients. However studies
on herbal therapy for starting multiple treatment are still inadequate.

KEYWORDS
Hemodialysis, chronic kidney disease, herbal therapy

Corresponding Author: FERAY GENER, gencerferay@gmail.com

1
GAZ NVERSTES, SALIK BLMLER FAKLTES

1294
 Poster Session 9
Submission ID: 1249

ESSENTIAL OIL AND FATTY ACID COMPOSITION OF CENTAUREA

SOLSTITIALIS SSP. SOLSTITIALIS

1 1 1 1 2 1
BURCU SMER TZN , TUBA GNEN , PELN TATAN , TUE FAFAL , BETL DEMRC , BJEN KIVAK

ABSTRACT
Centaurea is a widespread genus from Asteraceae family in Turkey(1). There are plenty of
researches about fatty acid and essential oil profiles of Centaurea species(2,3). Essential oils were
obtained using a Clevenger apparatus by hydrodistillation from Herba part of the plant. The essential
oil composition of the plant was identified by GC-MS using FID detector. 31 compounds representing
91.5% were identified. Hexadecanoic acid (50.2%) and tetradecanoic acid(10.1%) were found to be
the major compounds. For fatty acids, fatty acid methyl esters (FAMEs) were prepared. The fatty acid
compositions were analyzed by GC. Saturated fatty acids (SFAs) were totally 25.05%,
monounsaturated fatty acids (MUFAs) were 19.60% and polyunsaturated fatty acids were 19.86%.
The major compounds were found as oleic acid (18.54%), linoleic acid (10.07%), palmitic acid
(8.28%), stearic acid (6.82%) and - linoleic acid (6.75%). 1. Davis PH. Flora of Turkey. Edinburgh;
1975. p. 484585. 2. Senatore F, Arnold NA, Bruno M. Volatile components of Centaurea eryngiodes
Lam. and Centaurea iberica Trev.var. hermonis Bois. Lam., two Asteraceae growing wild in Lebanon.
Nat Prod Res. 2005;19(8):74954. 3. Erdogan T, Gonenc T, Cakilcioglu U, Kivcak B. Fatty Acid
Composition of the Aerial Parts of Some Centaurea species in Elazig, Turkey. Trop J Pharm Res. 2014
May 28;13(4):613.

KEYWORDS
Centaurea; essential oil; fatty acid

Corresponding Author: BURCU SMER TZN, burcusmer@gmail.com

1
EGE NVERSTES
2
ANADOLU NVERSTES

1295
 Poster Session 9
Submission ID: 1250

COMPOSITION OF ESSENTIAL OIL AND FATTY ACID OF

CENTAUREA PICHLERI SSP. PICHLERI

1 1 1 1 2 1
PELIN TATAN , TUE FAFAL , BURCU SMER TZN , TUBA GNEN , BETL DEMRC , BIJEN KIVAK

ABSTRACT
In Asteraceae family Centaurea genus is represented with 192 taxa in Turkey, 114 of which
are endemic. Many species of the genus Centaurea have traditionally been used for their
antirheumatic, diuretic, choleretic, stomachic, astringent, cytotoxic, antibacterial, antipyretic and tonic
properties. The essential oil compositions of some Centaurea species from Turkey have been
investigated. Generally, germacrene D, hexadecanoic acid, caryophyllene and caryophyllene oxide
were reported to be the major volatile components in the earlier studies. In Centaurea genus, Centaurea
pichleri ssp. pichleri is known as gelin dmesi, peygamber iei in Turkey. In this study, the
essential oil of Centaurea pichleri ssp. pichleri was obtained by hydrodistillation using a Clevenger-
type apparatus. The oil was analyzed by GC and GC/MS system. The components were seperated as
for adherencing to column and evaluated as for relative rate. Mass spectrums of each component were
taken. GC and GC/MS analyses of the essential oil from Centaurea pichleri ssp. pichleri were
determined the identification of 48 components representing 86.9 % of the oil. With this analysies, the
major component was found as hexadecanoic acid (31.4 %). Subsequent to this component, other
major components were caryophyllene oxide (6.4 %), spathulenol (6.2 %) and dodecanoic acid (4.5
%). In addition to this, fatty acid methyl esters (FAMEs) from Centaurea pichleri ssp. pichleri were
prepared for analysies of fatty acids. By this test the amount of saturated fatty acid (SFA) was found as
47.79 % with major fatty acid was stearic acid (18.64 %). The amount of monounsaturated fatty acid
(MUFA) was found as 16.88 % with major fatty acid was oleic acid (14.20 %). The amount of
polyunsaturated fatty acid (PUFA) was found as 21.29 % with major fatty acid was linoleic acid
(15.20 %). The results from this work were compared with the previous works in terms of essential
oils and fatty acids. References 1. P.H. Davis, R.R. Mill and K. Tan (1988). In: Flora of Turkey and
the East Aegean Islands, ed: P.H.Davis, Edinburh University Press, Edinburgh, Vol.10, pp.489-501 2.
A. Gner, N. zhatay, T. Ekim and K.H.C. Baer (2000). Flora of Turkey and the East Aegean
Islands. Edinburgh University Press, Edinburgh, Vol.11, pp.163. 3. G. Wagenitz (1975). Centaurea L.
In: Flora of Turkey and the East Aegean Islands, ed: P.H. Davis, Edinburgh University Press,
Edinburgh, Vol.5, pp.536. 4. T. Baytop (1999). Trkiye de Bitkilerle Tedavi (Gemite ve Bugn),
Nobel Tp Kitabevleri, stanbul, pp. 316. 5. E. Yeilada, E. Sezik, G. Honda, Y. Takaishi,Y. Takeda
and T. Tanaka (1999). Traditional medicine in Turkey. IX: Folk medicine in Northwest Anatolia. J.
Ethnopharmacol. 64, 195-210. 6. G. Zengin, A. Aktumsek, G.O. Guler, Y.S. Cakmak and Y. Kan
(2012). Composition of essential oil and antioxidant capacity of Centaurea drabifolia subsp. detonsa
Wagenitz, endemic to Turkey. Nat. Prod. Res. 26 (1), 1-10. 7. S.B. Erel, B. Demirci, S. Demir, C.
Karaalp, K.H.C. Baser (2013). Composition of the essential oils of Centaurea aphrodisea, C.
polyclada, C. athoa, C. hyalolepis, C. iberica. J.Essent. Oil Res. 25 (2), 79-84. 10. A. Altintas, Y.B.

Corresponding Author: PELN TATAN, pelintastan35@gmail.com

1
EGE NVERSTES
2
ANADOLU NVERSTES

1296
Kose, A. Kandemir, B. Demirci and K.H.C. Baser (2009). Composition of the essential oil of
Centaurea saligna. Chem. Nat. Comp. 45, 276-277.

KEYWORDS
Centaurea pichleri ssp. pichleri; essential oil; GC; GC/MS; fatty acid.

1297
 Poster Session 10
Submission ID: 686

P-CYMENE PRODUCTION FROM ORANGE PEEL ESSENTIAL OIL

ON DIFFERENT CATALYSTS IN THE SUPERCRITICAL ETHANOL

1 2
EMRE YILMAZOLU , MESUT AKGN

D
ABSTRACT
Orange is the most produced type of citrus fruits. Global orange production exceeded 64.9

TE
million tons in 2010/2011 season. The large part of the product is used as fruit or juice. On the other
hand, peel essential oil which is a by-product of the juice production is used in various fields like food,
cleaning, cosmetic and pharmacy directly or indirectly. Essential oil content is 1.450.16 ml per kg of
raw orange peel. 90-95% of the oil consists of limonene that can be transformed into different
compounds. In our study, it was worked that processing of orange peel oil which had been produced
EN
by cold pressure technique. Catalytic reactions of limonene carried out in a packed bed reactor using
Pt/Al2O3, Ni/Al2O3 and Pd/Al2O3 catalysts in over supercritical conditions of ethanol that its critical
point is ~241C and ~62 bar. The effects of temperature, pressure and retention time on yield were
investigated for each catalyst used. Isobaric pressure of experiments for temperature and retention time
ES
scanning was selected as 65 bar. All in all, Ni/Al2O3 was ineffective but Pd/Al2O3 was the most
effective catalyst for conversion of limonene. In the Pt/Al2O3 used experiments, it was seen that 10 s
retention time was not enough for difference of composition in the oil. In addition to that limonene
transformation occurred over the 280C, limonene ratio in the product decreased to 50% and 20% as
the temperature was 340C in the retention times of 30 s and 50 s, respectively. p-Cymene that was
PR

0.02% in the raw material, came to nearly 40% at 340C in the 50 s retention time. Thus, p-cymene
was the main component at the last point. Owing to Pd/Al2O3, it was achieved that conversion of
limonene was up to 100% in very short times. p-Cymene ratios were above the 80% at 340C in the all
retention times. It was detected that there was a special point for composition of the product in the
midst of 280C and 300C. Consequently, thanks to this catalyst, p-cymene was gained as the most
quickly and the most selectively in nearly all conditions studied. Points to consider about choosing the
T

temperature and the retention time for the process are very important with regards to being suitable for
purposes of using. Physical and chemical structures of the oil spoiled at the high temperatures. On the
O

other hand, limonene conversion was not adequate at the short retention times. While it was studied at
80 bar in the conditions of 300C and 50 s, it was found that conversion of limonene has a peak value
N

with Pt/Al2O3 and Pd/Al2O3 catalysts. In the end of reactions on Pt/Al2O3 at that pressure, limonene
ratio was 33% and p-cymene ratio was half of it. However Pd/Al2O3 was more effective and selective
than Pt/Al2O3. Thus, limonene hardly ever was found in the product at the same conditions when p-
cymene ratio was 80%.

KEYWORDS
Orange peel oil, Supercritical ethanol, Limonene, Pt/Al2O3, Ni/Al2O3, Pd/Al2O3

Corresponding Author: EMRE YILMAZOLU, emre.yilmazoglu@istanbul.edu.tr

1
STANBUL NVERSTES
2
YILDIZ TEKNK NVERSTES

1298
 Poster Session 10
Submission ID: 755

THE USE OF THYME AND PEPPERMINT (MENTHA PIPERITA) OF

THE LAMIACEA FAMILY, WHICH ARE OFTEN USED IN FOLK
MEDICINE, AS DYE SUBSTANCES IN WOOLLEN TEXTILE
PRODUCTS

1 1 1 1
HLYA KAYNAR , ASLIHAN GRBZER , ESRA UAR , NAZIRE GLAH KTK

ABSTRACT
All animals, plants and people live within a certain balance in nature. According to
archaeological findings from ancient times, people have always used plants, not only as food but also
in the treatment of health problems and for the dyeing of textiles. Due to the current intense use of
synthetic dyes, the use of natural dyes has become secondary. However, the importance of natural
dyes has increased with the emergence of the negative effects of synthetic dyes on human health. In
this study, an examination was made of the dye properties and colour and friction fastness values of
mint (Mentha piperita) and thyme (Thymus vulgaris) plants. According to the data obtained, the
colours obtained as a result of the use of acetic acid, copper II sulphate, zinc chloride, iron II sulphate,
potassium aluminium sulphate, potassium bi chromate, citric acid and tartaric acid mordants for thyme
and mint plants were determined to be olive green, black olive, cumin, coffee bean, kiwi peel, white
coffee, dark chocolate and cooked apple. The highest colour fastness values for mint were determined
as copper II sulphate (7), iron II sulphate (7), and potassium bi chromate (7) mordants. The highest
friction fastness value was obtained from iron II sulphate (5) mordants. The highest colour fastness
value for thyme was obtained from iron II sulphate (7) mordants and the highest friction fastness value
from iron II sulphate (5) mordants.

KEYWORDS
Thyme, Peppermint, Light fastness value, Friction fastness value, Mordan

Corresponding Author: ASLIHAN GRBZER, aslihan405@hotmail.com

1
CUMHURYET NVERSTES

1299
 Poster Session 10
Submission ID: 756

THE COLOURS AND FASTNESS VALUES OBTAINED FROM BASIL

(OCIMUM BASILICUM) AND LEMON BALM (MELISSA
OFFICINALIS) PLANTS OF THE LAMICIACAE FAMILY

1 1 1 1
HLYA KAYNAR , NAZIRE GLAH KTK , ESRA UAR , ASLIHAN GRBZER

ABSTRACT
Until the invention of synthetic dye substances, natural dyes, most of which have medicinal
and aromatic properties, were used for the purpose of dyeing in industries such as textiles, food,
medicine and cosmetics. With the introduction of synthetic dyes, their use increased and the demand
for natural dyes decreased. However, as a consequence of studies made, the carcinogenic properties of
synthetic dyes were revealed and that they could create serious prolems for human health. Therefore,
plant dyes that do not have negative effects on humans and other living organisms have come to the
fore again. Most plants which are known to have medicinal and aromatic properties also have a dye
property. In this study, an examination was made of the dye properties and colour and friction fastness
values of basil (Ocimum basilicum) and lemon balm (Melissa officinalis) plants. According to the data
obtained, dyeing made with basil (Ocimum basilicum) and lemon balm (Melissa officinalis) with
acetic acid, copper II sulphate, zinc chloride, iron II sulphate, potassium aluminium sulphate,
potassium bi chromate, citric acid and tartaric acid mordants gave colours of boiled chickpeas,
allspice, coffee bean, olive green, light green, arabic green, and potato peel. The highest colour
fastness values for basil were seen to be obtained with copper II sulphate, iron II sulphate, potassium
bi chromate and citric acid mordants. The highest friction fastness values were obtained with copper II
sulphate, potassium aluminium sulphate and citric acid mordants. For the lemon balm, the highest
colour fastness values were obtained with all the mordants and the highest friction fastness values with
acetic acid and citric acid mordants.

KEYWORDS
Basil ,Lemon Balm, Light fastness value, Friction fastness value

Corresponding Author: NAZRE GLAH KTK, gulsah-kutuk@windowslive.com

1
CUMHURYET NVERSTES

1300
 Poster Session 10
Submission ID: 765

PRODUCTION TECHNIQUES OF MADIMAK TEA (POLYGONUM

COGNATUM MEISSN)

1 1 1
EMRE HASTAOLU , MERYEM GKSEL SARA , BURAK DNEL

ABSTRACT
The production of medicinal and aromatic plants in the world and our country and the
consumption of the products obtained from these plants are increasing day by day. Medicinal and
aromatic plants are widely used in food industry, too. The main reason for these plants to be consumed
directly as food products is that these plants have antioxidant, antimicrobial properties. One of these
product groups is herbal teas used in immunotherapy or treatment of diseases due to factors that have
been in existence since prehistoric times. It is known that plants selected as medicinal herb tea
generally have high anticancer and antiaging properties in addition to their antioxidant activities.
Madimak (Polygonum cognatum Meissn) belongs to Polygonaceae family and is widely grown in the
inner Anatolia region. In middle Anatolia, it is collected from the nature and consumed as the basic
nutrient. Madimak plant is rich in antioxidants, flavonoids, phenolic compounds and carotenoids. The
polar and apolar antioxidant compounds found in ether, ethanol and water extracts have shown that
madimak is a potential antioxidant source. Among these samples, water extracts were found to have
the highest antioxidant activity. At the same time, it has been found that it is also effective against to
Gram positive bacteria. Especially, ether and ethanol extracts were found to be effective on
Staphylococcus aureus and Bacillus subtilis. In studies conducted on mice with colitis patients, the
madimak plant extract has the effects of reducing regulatory and oxidative stress in the intestinal
colon. Madmak tea can be used as an alternative to herbal teas. In this context; it is important to
cultivate the madimak plant and to produce madimak tea to protect both the cultural richness and to
create the regional market.

KEYWORDS
madimak (Polygonum cognatum Meissn), antioxidant, antimicrobial, drying, herbal tea

Corresponding Author: BURAK DNEL, burakdincel@cumhuriyet.edu.tr

1
CUMHURYET NVERSTES

1301
 Poster Session 10
Submission ID: 1251

MACRO, TRACE AND TOXIC ELEMENTS OF 4 DIFFERENT EDIBLE

WILD PLANTS FROM KARADENIZ REGION

1 2 1 1
ATILLA IMEK , AYLIN IMEK , MELIKE DEMIRKOL , EMRE TURAN

ABSTRACT
The study was conducted to assess the content (mg kg-1 fresh wt.) of macro, trace and toxic
elements in the 4 different edible wild plants. The percentage of dry matter and ash ranged from 6.77
to 20.56 and 0.79 to 2.26, respectively. The contents of Ca (1074), Fe (160.5), Mn (18.21), Ni (6.11),
B (2.61), Cr (0.93), Co (0.50), Mo (1.80), Be (0.009) and Se (0.076) of U. diocia were richer than in
other plants. Other hand, K (7742), Mg (954), Al (10.79), V (9.77) and Ag (0.109) in T. orientalis, Zn
(12.47), Cu (9.98) and Ba (130.3) in O. umbellatum were taken the highest value. Also As, Hg, Tl, Cd
and Pb were determined mg kg-1 level in fresh plants. Conversely, antimony (Sb) in wild plants could
not be detected by ICP-MS. The results of statistic analysis of forty plants showed that moisture, total
dry matter, ash, K, Ca, Mg, Fe, Zn, Cu, Mn, Ba, Ni, Al, V, B, Cr, Co, Mo, Be, Se, Ag, As, Tl, Cd and
Pb contents were varied significantly compared to plant species except for Na and Hg (P<0.05).
Consequently, wild plants may be used as popular vegetables in many people diet as a source of
minerals (Fe, Cu, Mn, Cr, Mo, K, Zn and Mg). Excessive plant consumption may be adversely
affected human health with Cd, As, Hg, Tl and Pb.

KEYWORDS
Edible wild plants, Macro-Microelements, Toxic elements, ICP, Traditional foods

Corresponding Author: ATLLA MEK, atillasimsek62@yahoo.com

1
ORDU NVERSTES
2
ORDU L GIDA TARIM VE HAYVANCILIK MDRL

1302
 Poster Session 10
Submission ID: 1252

ANTIOXIDANT ACTIVITY OF GILABURU

1 1 1
AYE FDAN , RECEP PALAMUTOLU , CEMAL KASNAK

ABSTRACT
Plants are used for therapeutic purposes since very ancient times. In our country, some plants
are referred as medicinal herbs among the people, also used in the treatment of many diseases.
Viburnum opulus (gilaburu) belongs to Caprifoliaceae family. The stem, bark and fruits of Viburnum
opulus has a wide usage in pharmacology. Gilaburu are naturally grown in the provinces of Kayseri,
Bursa, Tokat, Sivas, in our country. Many compounds have been identified in the bark, shell and fruit
of the gilaburu. Gilaburu has anticarcinogenic, antimicrobial and antioxidant activities because of the
organic acids content. Antioxidants bind to free radicals in the body and stop many adverse health
effects. Traditionally this fruit juice is consumed especially in the province of Kayseri. There are
beliefs in this region that gilaburu is good for stomach discomfort and has the capacity to dissolve
kidney stones. This fruit is also called "kidney doctor" in this region. The fruit is collected by hand,
from October according to its maturity status. The aim of the study is to compile information about
antioxidant capacity of gilaburu, its health effects and usage areas among the public.

KEYWORDS
Gilaburu, Antioxidant, Health

Corresponding Author: RECEP PALAMUTOLU, rpalamutoglu@aku.edu.tr

1
AFYON KOCATEPE NVERSTES

1303
 Poster Session 10
Submission ID: 1255

ESFAND SEED AND HEALTH

1 1 1
MERVE KANICIOLU , RECEP PALAMUTOLU , CEMAL KASNAK

ABSTRACT
Peganum harmala; It is a plant that opens greenish-white flowers between May and August,
which grows in semi-arid conditions in the steppe fields. It is grown in Konya, Ankara, Sivas, Nide,
Kayseri, Afyon in our countryand in the USA, North Africa and Southern Europe in the world.
Peganum harmala seeds contain alkaloids (harmin, harmal, harmalol, harmaline) which are in the -
carboline ring structure. It has been reported that these alkaloids has oxidative stress reducing,
antidepressant, antithrombocyte aggregation effects. Antimicrobial, antimicrobial, antitumor, and
antifungal effects were reported in some researches. Peganum harmala seeds are commonly known as
zerlik, an amulet made from fruits, and incense made from seeds for evil eye. Researchers continue
to work on the bioactive components of plant materials in recent years.

KEYWORDS
Peganum harmala, health, esfand seed

Corresponding Author: RECEP PALAMUTOLU, rpalamutoglu@aku.edu.tr

1
AFYON KOCATEPE NVERSTES

1304
 Poster Session 10
Submission ID: 1256

THE ROLE OF SECONDARY METABOLITES IN MEDICAL AND

AROMATIC PLANTS IN ORGANIC AGRICULTURE

1
TUBA ESERKAYA GLE

ABSTRACT
Until 21st century, the main purpose was to increase the yield in agricultural production to
cover the food needed by the increasing population, and improving the quality of the crop and
protecting the natural resources, and the health of humans and the environment were considered in the
second rank. With the intense input application to receive the highest yield from a unit area, the soil
structure was destructed, and as a result of the accumulation of harmful chemicals and with the
pollution of underground waters, sustainable agriculture was endangered. The disruptive effects of
extensive agriculture that destroys the ecological balance in time increased the search for alternative
methods, and in this step, the production model which was called as organic agriculture was put
forward with the purpose of establishing the disrupted balance. Organic agriculture is the certified
agricultural production in which chemical inputs are not used and controlled from the initials steps
until the end. For this reason, alternative fight that may be used in organic agriculture have become
more important in time (Badat and Kark, 2009; Yetgin, 2010). The use of medical and aromatic
plants is increasing in medicine and drug industry and also in food and cosmetics industry. The studies
conducted in this field showed that medical and aromatic plants may be used for many purposes in
biological fight in organic agriculture due to the secondary metabolites in them (Mder et al., 2002;
Topuz and Madanlar, 2014). The most important reason of the emphasis on secondary metabolic is
that they are natural compounds, and do not release toxic substances, and are decomposed in a
relatively shorter time thus not causing pollution in the soil and water, do not form layers on the
products that threaten the human health and have disincentive or lethal effects on many weed species
or plant diseases (Erler, 2000). Allelopathic effects of medical and aromatic plants can suppress the
weeds in agricultural areas without the need for a chemical fight. In many studies, it was revealed that
various essential oils applied at different concentrations affected the germination and growth of many
weeds in a negative way (Totey et al., 1994; Dudai et al.., 1999; Karaltn et al.., 2001; nen, 2003).
Today, it has been reported that nearly 2000 plant species have the property of being used in the fight
against harmful insects; however, especially the essential oils and extracts of 26 of them may be used
as insecticide against the larvae of harmful insects. The most common ones of these plants are
Azadirachta indica, Derris eliptica, Acarus calamus, Rhyania speciosa, Melia spp., Chrysanthemum
cinerariifolium (Ahmed et al., 1984). Pavela (2004) used the methanol extracts of eight medical and
aromatic plants to test the insecticide effects of them on corn cotton worm (Spodoptera littoralis)
larvae, and reported that all extracts had toxic effects on larvae. It was also reported that the extracts of
basil (Ocimum basilicum), sweet marjoram (Origanum majarona) and medical sage (Salvia officinalis)
had extremely high larval toxicity. It was also reported that the extracts of sage (Salvia officinalis L.)
and rosemary (Rosmarinus officinalis L.) are used successfully in the fight against potato beetle
(Leptinotarsa decemlineata Say.) (Kara et al., 2014). It was also determined that medical and aromatic

Corresponding Author: TUBA ESERKAYA GLE, tuba.eserkaya@gmail.com

1
KARAMANOLU MEHMETBEY NVERSTES-TBMYO

1305
plants have important effects in terms of water and soil retention of plants. In a study conducted on
this purpose, various elements were analyzed from the upper and lower layers of the soil in which Iris
histrioides Foster grew in the Black Sea Region, and it was reported that the % N, P and K
components in the upper layer of the soil were high (Kandemir and Engin, 2000). Again, a study was
conducted by Balak and Misra (2004), and it was reported that German chamomile played important
role in breeding soil. In addition, the industrial wastes of medical and aromatic plants may be used as
organic fertilizers (Badat and Kark, 2009). With the help of the chemicals released by medical and
aromatic plants, it is possible to attract the harmful insects that are in a certain population intensity in a
field to trap plants, and decrease their population intensity by mixing them with the soil (Gl et al.,
1997; Hilker and Meiners, 2011). In previous studies, it was reported that mustard was used
successfully both as a trap plant and as a green fertilizer in decreasing the intensity of grain and beet
cyst nematodes in the fields. These plants are sensitive to these nematodes and ensure that cysts are
activated and make the larvae invade the roots and when they are mixed with the soil in accurate time,
they killed young nematodes (zer et al., 2003; Hilker and Meiners, 2011). In this study, the data on
the potential use of medical and aromatic plants in biological fight in organic agriculture have been
compiled.

KEYWORDS
Allelopathic Effect, Secondary Metabolite, Organic Agriculture, Trap Plant

1306
 Poster Session 10
Submission ID: 1257

BIOTECHNOLOGICAL METHODS AND BREEDNG IN MEDICAL

AND AROMATIC PLANTS

1
TUBA ESERKAYA GLE

ABSTRACT
The lack of adequate variety and seeds of medical and aromatic plants whose culture is
performed makes it difficult to have them under culture studies and sustain them. For this reason,
studies that will be conducted on plant breeding for these plants become extremely important (Baydar
and Telci, 2015). However, the breeding of medical and aromatic plants has fallen back when
compared with the other culture plants. One of its reasons is the difficulty in developing breeding
methods that are specific to each plant due to the extensive variety of medical and aromatic plants. In
addition, the data on quality parameters on agricultural properties and on relevant genes are extremely
limited. The second important reason is the fact that these plants are grown in limited areas and for
this reason, the final situation of the investments and the return of the breeder share are extremely low.
Aside from these, the necessity for making the quality analyses, the expensive equipment and
substances needed make it difficult to breed these plants (Arslan et al., 2015). Medical and aromatic
plants are used as raw materials in food, medicine, cosmetics and many other industries. (Baer, 1997;
1998). For this reason, these industrial branches require the use of materials that are within certain
standards, and therefore, in order to obtained high-yield and high-quality raw drog from medical and
aromatic plants, there is a need to develop the variety of standards. In breeding medical and aromatic
plants, not only the drog yield but also the active substance yield is important at a great deal. In
breeding hashish (Papaver somniferum), breeding methods aim to improve the alkaloids like
morphine, codeine, thebaine, noscapine and papaverin as well as improving the capsule yield
(Gm and Arslan, 2008). In this way, higher-level alkaloid yield may be achieved without being
dependent on cultivation areas (Baydar and Telci, 2015). Completely eliminating or decreasing the
amount of substances that have negative effects on health in medical and aromatic plants is among the
most important purposes of breeding works. As an example, it is possible to give the study conducted
to breed species of medical sage (Salvia officinalis L.) that do not have camphor due to its toxic effects
or that have camphor rates below 0.5% (Dudai et al., 1999). In plants like mint and melissa, the
breeding works continue for the purpose of making these plants acquire endurance against disease
factors, and in rosemary, the works are still being continued to make it acquire endurance against cold.
In addition, it is also aimed to detect medical and aromatic plants that have stronger antimicrobial and
antioxidant effects ad to develop new genotypes with increased effects (Yarnell and Abascal, 2004;
Altnda and Aslm, 2005; Hussain, 2011; Baydar and Telci, 2015). The breeding works in medical
and aromatic plants are close and similar to those applied in classical breeding methods. Aside from
these, many works like genotype detection, adulteration and quality control, GDO test, genetic
relations may be done by using DNA markers. In breeding works, natural plant populations that are
extremely important in genetical terms and local (village) species are used. One of the most important
advantages of the breeding of the plants is their having a wide variation and being prone to develop

Corresponding Author: TUBA ESERKAYA GLE, tuba.eserkaya@gmail.com

1
KARAMANOLU MEHMETBEY NVERSTES-TBMYO

1307
with a simple selection. 70-80% of the medical and aromatic plants in the world are obtained from
natural populations, local species or from introduction materials with simple selection method. (Pank,
2006; Arslan et al., 2015). Today, bio-technological methods are used for the purpose of performing
plant production with higher quality and quantity in a more economical way by solving the problems,
which are not solved with known traditional methods, in this process (Uar and Turgut, 2009). In
breeding medical and aromatic plants, bio-technological methods are also made use of as well as
classical breeding methods. Detecting and improving the genotypes that can endure salinity, drought,
diseases and hat will bring several benefits such as secondary metabolite production with plant cell
cultures. Biotechnological methods are used successfully especially in the production of secondary
metabolites in in vitro conditions, in eliminating environmental actors, producing crops in the desired
amount and standard quality, or in producing semi or fully synthetic secondary metabolites (Baydar,
2013). In this study, the data on breeding methods and biotechnological methods applied on medical
and aromatic plants have been compiled.

KEYWORDS
Medical and Aromatic Plants, Breeding, Secondary Metabolite.

1308
 Poster Session 10
Submission ID: 1259

THE EFFECTS OF DIFFERENT CULTIVATION AND ECOLOGICAL

CONDITIONS ON YIELD AND QUALITY OF CARTHAMUS
TINCTORIUS L. ( SAFFLOWER)

1
UMMAHAN Z ARIK

ABSTRACT
Carthamus tinctorius L. (Safflower) is an industrial plant with a high medical potential.
Safflower give positive results in the treatment of acute appendicitis, women's period, cardiovascular
disease, fever reduction, asthma, osteoporosis, rheumatoid arthritis. The cultivation of the Safflower is
important because of its industrial use and pharmacological properties. Cultural practices vary from
region to region. Depending on the ecological conditions of each region, types of the cultivation
changes. Sowing time and sowing frequency are effective on yield. Generally, the autumn planting is
more suitable from spring planting for the development of plant characteristics and seed yield.
Nitrogen , phosphorus and humic acid applications were positively effect oil yield . This review was
carried out to determine the effect of different cultivation and ecological conditions on yield and
quality of Safflower.

KEYWORDS
Carthamus tinctorius, Safflower, cultivation, yield, quality

Corresponding Author: UMMAHAN Z ARIK, ummahanoz48@gmail.com

1
CELAL BAYAR NVERSTES ALAEHR MESLEK YKSEKOKULU TIBB VE AROMATK BTKLER
PROGRAMI

1309
 Poster Session 10
Submission ID: 1260

BIOACTIVE PROPERTIES AND ANTIMICROBIAL ACTIVITY OF

SOME HYPERICUM SPECIES GROWING WILD IN BLACK SEA
REGION OF TURKEY

1 1 2 3 1
LATIFE BETL GL , NILGN ZDEMR , OSMAN GL , CNEYT IRAK , AHMET HILMI ON

ABSTRACT
The aim of this study was to investigate total phenolic contents, antioxidant and antimicrobial
activities of some Hypericum species growing wild in Blacksea Region of Turkey. For determining all
these functional properties, ethanolic extractions of both leaves and flowers of four wild Hypericum
species (H. perfoliatum L., H. aviculariifolium Jaup. and Spach subsp. depilatum (Freyn and Bornm.)
Robson var. depilatum, H. origanifolium Wild. and H. linarioides Bosse) were performed separately
by maceration method. Total phenolic contents of these flower and leaf extracts were found between
148.04-83.89 and 202.83-48.03 mg gallic acid equivalents (GAE) /g dry weight, respectively. H.
linarioides was determined as the Hypericum specie getting highest total phenolic content. Antioxidant
activities of both flower and leaf extracts were determined by DPPH assay. According to the results,
antioxidant activities of the flower extracts were determined between 24.94-17.14 mg trolox
equivalent /g dry weight and for the leaves, the values were found between 39.35-8.63 mg trolox
equivalent /g dry weight. The highest antioxidant activity was observed on the leaf extract of H.
linarioides. Antimicrobial activities of the extracts were evaluated by minimal inhibitory
concentrations (MIC) against 9 pathogenic and/or food spoiling bacterial strains (Staphylococcus
aureus, Pseudomonas aeruginosa, Bacillus pumilis, B. subtilis, B. licheniformis, Listeria innocua,
Esherichia coli, B. cereus and L. monocytogenes). MIC results show that the flower extracts generally
exhibited higher antimicrobial activity than the leaf extracts. And especially, the flower extract of H.
perfoliatum showed strong inhibition effect against all strains except E. coli.

KEYWORDS
Hypericum spp., total phenolic content, antioxidant activity, antimicrobial activity

Corresponding Author: LATFE BETL GL, latife.betul@omu.edu.tr

1
ONDOKUZ MAYIS NVERSTES, MHENDSLK FAKLTES, GIDA MHENDSL BLM
2
ONDOKUZ MAYIS NVERSTES, YELYURT DEMR-ELK MESLEK YKSEK OKULU, GIDA
TEKNOLOJS PROGRAMI
3
ONDOKUZ MAYIS NVERSTES, BAFRA MESLEK YKSEK OKULU, BTKSEL VE HAYVANSAL RETM
BLM

1310
 Poster Session 10
Submission ID: 1261

EFFECTS OF DIFFERENT PLANT DENSITY AND CUTTING TIMES

ON YIELD OF STEVIA

1 1 1 2
LEYLA SEZEN TANSI , ELNAZ SAMEDPOURRIGANI , SELIN GEDIK , AMIR SOLTANBEIGI

ABSTRACT
Stevia (Stevia rebaudianaBertoni) is perennial, shrub form plant, belonging to Asteraceae. It is
indigenous to Paraguay and Brazil. Stevia is a natural sweetening, and it has using potential to diets of
obesity patients with non-caloric speciality and treatment of diabetes with insulin secretagogue
speciality. In this study, the effects of different planting spaces and two cutting times on yields of two-
year old stevia plant were investigated. The highest dry leaf yield (515.96 kg/da) was obtained from
blooming stage, cuttingsin spring and 30x60 cm planting density.

KEYWORDS
Stevia rebaudiana, planting space, cutting times.

Corresponding Author: SELN GEDK, sgedik@cu.edu.tr

1
UKUROVA NVERSTES, ZRAAT FAKLTES, TARLA BTKLER BLM.
2
AFYON KOCATEPE NVERSTES, AHMET NECDET SEZER KAMPS, GIDA KONTROL ARA. VE UYG.
MERKEZ

1311
 Poster Session 10
Submission ID: 1262

SOME FUNCTIONAL AND TEXTURAL PROPERTIES OF GUAR AND

XANTHAN GUM UTILIZED EGGLESS CAKE

1 1
YUSUF DURMU , ZEKA TARAKI

ABSTRACT
Cake; a ready-to-eat food product produced in many countries in a wide variety of shapes,
formulations and high nutritional value. Eggs are often used in cake formulations, which can cause
allergic reactions in some people, especially in children. In this study, eggless plain cakes were
produced using guar (GG) and xanthan (XA) gums with ratios of 0.0%, 1.0%, 1.5% and 2.0% and
some functional properties of cakes were examined. The cakes were stored for 48 hours and some
properties were determined at 1., 24. and 48 hours. GG and XA gums did not significantly affect the
cooking efficiency (p>0.05). It was observed that the pH value decreased with the addition of gums in
all samples. It has been found that the pH value decreased during storage for GG 1.0% and XA 1.0%
samples. According to acidity results acidity did not change in control samples during storage. The
usage of gums has increased the acidity of all samples. Acidity generally increased during storage and
it was found that acidity of GG samples was higher than that of XA samples. It was determined that
brightness of samples slightly increased during storage according to color measurements. Gum
addition showed an increase in brightness. With the use of gums, the a* value is generally increased,
while the maximum redness was reached by XA 2.0%. While b* values of XA were found to be lower
than that of GG samples, no significant changes were observed during storage in b* values (p>0.05).
According to the texture results it was determined that the GG samples were harder than the XA
samples for each time period during storage. GG 1.5% and GG 2.0% were statistically higher than
other samples at 1. hour (p<0.05). It has been recorded that flexibility increased during storage. The
addition of XA resulted in more sticky cakes than GG. It was observed that the gummy property
increased gradually during storage while the GG samples more gummy as average to the XA for each
storage period. The chewiness of cakes increased during storage and it was determined that the use of
gums increased the chewiness. Generally, chewiness values of GG samples were higher than those of
XA samples. Gum added cakes were found to be more elastic than the control samples and it was
determined that XA increased the elasticity more than GG. The lowest elasticity was determined in the
control samples at the 1. and 24. hours of storage. According to the sensory evaluations, the control
samples were most favored in terms of taste-aroma. GG and XA samples were similarly liked by the
panelists. There were no significant differences in cake color, pore structure and odor evaluations
among the cakes in sensory evaluations.

KEYWORDS
Cake, gum, guar, xanthan

Corresponding Author: YUSUF DURMU, yusuf_bm@hotmail.com

1
ORDU NVERSTES ZRAAT FAKLTES GIDA MHENDSL BLM

1312
 Poster Session 10
Submission ID: 1263

USE OF SOME ESSENTIAL OILS IN GRAPE STORAGE

1 2
UMMAHAN Z ARIK , CUMA ARIK

ABSTRACT
Medical and aromatic plants are generally used in fields such as food, spice , medicine,
beverage and cosmetics. Nowadays , there are numerous studies on the antimicrobial and insecticidal
properties of essential oils and extracts from these plants. The investigations indicate that medicinal
and aromatic plants can be used to increase the shelf life, especially in fruit preservation. This studies
show that the essential oils extracted from medicinal and aromatic plants such as thyme, caraway and
carnation are effective in the suppression of fungi such as Botrytis cinerea, Aspergillus niger,
Alternaria alternata, Colletotrichum gloeosporioides, Lasiodiplodia theobromae, Penicillium
digitatum, Phomopsis viticola and Rhizopus stolonifer which reduce the shelf life in grape
preservation, but it does not negatively effect the sensory properties of the grape. This review is
intended to reveal the use of certain essential oils in grape storage.

KEYWORDS
Grape, storage, medical and aromatic plants, essential oil, Botrytis cinerea

Corresponding Author: UMMAHAN Z ARIK, ummahanoz48@gmail.com

1
CELAL BAYAR NVERSTES ALAEHR MESLEK YKSEKOKULU TIBB VE AROMATK BTKLER
PROGRAMI
2
CELAL BAYAR NVERSTES ALAEHR MESLEK YKSEKOKULU BACILIK PROGRAMI

1313
 Poster Session 10
Submission ID: 1264

AS A NUTRACEUTCAL: CHLORELLA

1 1 1
NURCAN ELK , RECEP PALAMUTOLU , CEMAL KASNAK

ABSTRACT
Today, nutriceuticals is described as The food that provides benefical effects by generating
one or more physiologic functions like enhancing healty or creating decrease the risk of being exposed
to some disesases. Physiologic benefits of the nutriceuticals are determined and revealed the usage
utility nutraceutical contributions that show the protection to any cronic diseases suchlike drugs-one of
them is microlgea originating from sea. Because of including sufficient level of functional ingredients
such unsatured fatty acids, omega-3 fatty acid, B-caroten and other pigments, sulfates,
polysaccharide(antiviral) and sterols(antimicrobial); the microalgea are used quite frequently.
Chlorella is a type of microalgae, is known the most common species in this group by considering the
farming. Also, it is used as a nutraceutical in medicine, cosmetics and pharmaceutics. It is absiously
seen from some scientists works that Chlorella which has 3 types ellipsoidea, pyrenoidasa and
vulgaris has positive effects on human health like regulating blood pressure, decreasing the level of
cholesterol, avoiding some viral diseases and reinforcement of immune system. Since Chlorella has
these properties, it became popular almost all over the world as a functional and nutraceutical food.

KEYWORDS
chlorella, cancer, chlorella vulgaris, nutraceutical, functional food

Corresponding Author: RECEP PALAMUTOLU, rpalamutoglu@aku.edu.tr

1
AFYON KOCATEPE NVERSTES

1314
 Poster Session 10
Submission ID: 1266

EFFICACY OF SUCROSE AND THIDIAZURON ON IN VITRO SHOOT

REGENERATION OF FENUGREEK (TRIGONELLA FOENUM-
GRAECUM L.)

1 1 1 2 3
BEHIYE BRA TABAI , ESRA KAVCI , ARIFE KITRI0 , SIBEL DAY , MUHAMMAD AASIM , KAHLID MAHMOOD
4
KHAWAR

ABSTRACT
Fenugreek (Trigonella foenum-graecum L.) is an important medicinal and edible legume
which is also used as spice, forage and green manure. It contains important bioactive compounds like
glactomannan, saponins, mucilage, alkaloids and volatile oil. Due to its highly economic vvalue, there
is need to apply biotechnological tools for variety improvement and obtaining secondary metabolites.
Keeping in view, the present study was designed to develop an efficient regenration protocol of
fenugreek. Cotyledonary nodes and leaf explants taken from 18-20 days old seedlings were cultured
on phytagel solidified MS medium enriched with different concentrations of sucrose (1.5, 3.0, 4.5 and
6.0%). Medium was also enriched with 0.40, 0.80 and 1.20 mg/l Thidiazuron (TDZ) and 0.20 mg/l
NAA. 100% callus induction was recorded on both explants with no shoot regneneration from leaf
explants. However, cotyledonary nodes genertaed 100% shoot buds which later turned into shoots.
Maximum number of 18.75 shoot buds were recorded on medium supplemented with 0.40 mg/l
TDZ+0.20 mg/l NAA with 1.5% sucrose concentration. Explants were subcultured after 6 weeks of
culture to MS medium which turned the shoot buds into well developed shoots. Explants were left in
Magneta vessels for further growth for other studies.

KEYWORDS
Callus, In vitro, Fenugreek, Medicinal, Shoots, Regeneration

Corresponding Author: MUHAMMAD AASM, mshazim@gmail.com

1
DEPARTMENT OF BOTECHNOLOGY, FACULTY OF SCENCE, NECMETTN ERBAKAN UNVERSTY,
KONYA, TURKEY
2
DEPARTMENT OF FELD CROPS, FACULTY OF AGRCULTURE, ANKARA UNVERSTY, ANKARA
3
DEPARTMENT OF BOTECHNOLOGY, FACULTY OF SCENCE, NECMETTN ERBAKAN UNVERSTY,
KONYA, TURKEY
4
DEPARTMENT OF FELD CROPS, FACULTY OF AGRCULTURE, ANKARA UNVERSTY, ANKARA

1315
 Poster Session 10
Submission ID: 1267

POTENTIAL USES OF ESSENTIAL OIL COMBINATIONS AS

SURFACE DISINFECTANT

1 1 1
LKIN YCEL ENGN , GLDEN KILI , EYDA GL

ABSTRACT
In the present work, the antimicrobial activity of essential oils including cinnamon,
peppermint, oregano, nettle seed and clove oils, and different combinations of these essential oils were
investigated. In the first stage of the study, the antimicrobial activity of essential oils against seven
bacterial strains (Listeria monocytogenes, Enterococcus faecalis, Bacillus subtilis, Staphylococcus
aureus, Escherichia coli O157:H7, Salmonella Typhimurium, Escherichia coli) was determined by
minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) assays.
MIC value was determined for each microorganism using a 96-well microtiter plates. Essential oils,
except peppermint and nettle seed oils, exhibited inhibitive effect on all test microorganisms at
concentrations ranging between 0.156 and 10% (MIC, v/v). The highest inhibitive effect on test
cultures was observed in oregano oil with MIC values in the range of 0.156-0.625%. When the
essential oils were used in combinations, the MIC values of both prepared formulations were ranged
between 0.625-2.5% and 0.312-2.5%. In the second stage of the study, the most effective formulation
containing oregano, cinnamon and clove oil (1:1:1) was used as the surface disinfectant at 10, 20 and
30% concentrations for 1 and 5 min treatment time. The analyze results showed that the selected
formulation used at 10% concentration was effective against E. coli inoculated on working surface
(4.59 log CFU/25 cm2), which reduced the cell numbers to an undetectable level for 1 min
application. On the other hand, 96% alcohol application reduced the cell numbers by 1.15 and 1.30 log
units for 1 and 5 min treatment times, respectively. When the effects of solutions were compared in
terms of the numbers of total mesophilic aerobic bacteria that naturally found on surfaces, the
disinfection effect of the prepared essential oil formulation was found higher than 96% alcohol. These
results showed that essential oils used in combinations might be a good alternative in the disinfection
of surfaces.

KEYWORDS
essential oils, combination, antibacterial, surface disinfection

Corresponding Author: LKN YCEL ENGN, ilkinyucel@yahoo.com

1
EGE NVERSTES

1316
 Poster Session 10
Submission ID: 1268

EFFICACY OF EXPLANT AGE, SUCROSE AND THIDIAZURON ON

IN VITRO SHOOT REGENERATION OF FENUGREEK
(TRIGONELLA FOENUM-GRAECUM L.)

1 1 1 2 3
ESRA KAVCI , BEHIYE BRA TABAI , MUHAMMAD AASIM , SIBEL DAY , ALLAH BAKHSH , KHALID
4
MAHMOOD KHAWAR

ABSTRACT
Fenugreek (Trigonella foenum-graecum L.) belongs to Fabacae family is an important
medicinal plant thats is used as vegetable and making pickles in Indo-Pak subcontinet. It contains
important biologically active secondary metabolites like alkaloids, glactomannan, saponins, mucilage,
and volatile oil that increase the value of the plant. However, there is need to use biotechnological
tools in order to produce more elite plants and to isolate secondary metabolites. In this study, we used
10 and 20 days old cotyledonary nodes explants from in vitro grown seedlings and cultured on gelrite
solidified MS medium fortified with 0.40, 0.80 and 1.20 mg/l Thidiazuron (TDZ)+0.20 mg/l NAA
with different sucrose concentrations (1.5, 3.0, 4.5 and 6.0%). Callus induction followed by somatic
embryogenesis (100 %) was recorded after 4 weeks of culture which later on turned into shoots or
shoot buds. 20 d old explant regenerated more number of shoots compared to 10 d explant. Maximum
number of shoots/shoot buds were achievd on medium fortified with 0.80 mg/l TDZ+0.20 mg/l NAA
provided with 4.5 % sucrose. Explants were left for growth for further studies.

KEYWORDS
Fenugreek (Trigonella foenum-graecum L.) belongs to Fabacae family is an important
medicinal plant thats is used as vegetable and making pickles in Indo-Pak subcontinet. It contains
important biologically active secondary metabolites like alkaloids, glact

Corresponding Author: MUHAMMAD AASM, mshazim@gmail.com

1
DEPARTMENT OF BOTECHNOLOGY, FACULTY OF SCENCE, NECMETTN ERBAKAN UNVERSTY,
KONYA, TURKEY
2
DEPARTMENT OF FELD CROPS, FACULTY OF SCENCE, ANKARA UNVERSTY, ANKARA
3
DEPARTMENT OF AGRCULTURAL GENETC ENGNEERNG, FACULTY OF AGRCULTURAL SCENCES AND
TECHNOLOGES, NGDE UNVERSTY, NGDE, TURKEY
4
DEPARTMENT OF FELD CROPS, FACULTY OF SCENCE, ANKARA UNVERSTY, ANKARA

1317
 Poster Session 10
Submission ID: 1269

SOME MEDICINAL AND AROMATIC PLANTS VISITED BY BUMBLE

BEES IN TURKEY

1 1
REFIKA GNALP , NEVIN KESKIN

ABSTRACT
Bumble bees (Bombus sp.) are large, colorful, ubiquitous pollinators which found throughout
the Holarctic, Oriental, and Neotropical regions of the world, especially in alpine and arctic zones.
They like to be found in open meadows, which provide abundant amounts of nutrients throughout the
flight, and tend to go with long corolla bushes belonging to the family of Lamiaceae and Fabaceae in
these habitats. Turkey is one of the richest countries in the world in terms of medicinal and aromatic
plants, and there are about 12 000 plant taxa naturally grown. Of these, 3750 are endemic and the
endemism rate is very high when compared to European countries. Hundreds of plant species naturally
grown in Turkey, especially endemic plants that have high medicinal and aromatic value. About 500
plant species in Turkey are being utilized in the context of folk medicine or traditional medicine
applications. In this study, 10 of the medicinal and aromatic plants that Astragalus sp., Thymus fallax
Fisch.&Mey., Salvia officinalis L., Centaurea solstitialis L., Lavandula angustifolia Mill., Rosmarinus
officinalis L., Papaver somniferum L., Pimpinella sp., Acacia cyanaphylla L., Shymphytum sp. were
handled and their relation to bumble bees were compiled from various sources.

KEYWORDS
Medicinal and aromatic plants, Bumble bees, Turkey

Corresponding Author: REFKA GNALP, refika.gunalp@hacettepe.edu.tr

1
HACETTEPE NVERSTES

1318
 Poster Session 10
Submission ID: 1270

DIETARY FIBERS AND BIOACTIVE COMPOUNDS IN GRAPE

POMACE

1 1 1 1
ALI SABIR , FERHAN SABIR , ALPER KKBASMACI , OMAR TURHAN JALIL JALIL

ABSTRACT
Lifestyle changes, including healthy diets and exercise, constitute the most powerful tools to
fight against many chronic diseases (Urquiaga et al. 2015). Mediterranean diets are considered as
valuable preventive measures as several studies have proven that their intake is associated with lower
occurrence and prevalence of chronic diseases (Keys 1970) and longer life expectancy (Trichopoulou
2004). Antioxidants, fiber, unsaturated fats and phytochemicals are the bioactive components of
Mediterranean diets that apparently support the human health (Simopoulos 2001). Grape pomace
(GP), a rich source of antioxidant and fiber, is used as an ingredient for functional foods and as a
dietary supplement to increase the intake of dietary fiber and bioactive compounds. Jimnez et al.
(2008) found that fibers from GP show high reducing efficacy in lipid profile and blood pressure due
to combined effect of dietary fiber and antioxidants. GP as antioxidant dietary fiber also helps to
enhance the gastrointestinal health of the human by promoting a beneficial microbiota profile (Pozuelo
et al., 2012). Antioxidant dietary fiber may be incorporated with flour for making high dietary fiber
bakery goods, while the polyphenols in antioxidant dietary fiber could contribute as antioxidant for
improving aroma, color and taste of the product. For example, GP was mixed with sourdough for rye
bread (Mildner-Szkudlarz et al. 2011) and grape seed flour for pancakes and noodles (Rosales Soto,
Brown and Ross, 2012). Aside from promoting human health, GP plays important role as
antimicrobial agent to maintain the shelf-life of foods. Goni et al. (2009) and Snchez-Alonso et al.
(2007) revealed that GP added into minced fish and chicken breast delayed the lipid oxidation. Also,
GP extract exhibited antimicrobial effect against foodborne pathogens when added into beef patties
(Sagdic et al. 2011). There are increasing interests in applying GP, grape processing wastes, as
functional food ingredients because they are rich source of dietary fiber, and most of the beneficial
bioactive compounds are remained in the byproducts.

KEYWORDS
antioxidant dietary fiber, grape pomace, bioactive compounds

Corresponding Author: AL SABIR, asabir@selcuk.edu.tr

1
SELCUK UNVERSTY, AGRCULTURE FACULTY, HORTCULTURE DEPARTMENT, KONYA, TURKEY

1319
 Poster Session 10
Submission ID: 1271

LATHYRUS CZECZOTTIANUS: CAN BE NEW SOURCE OF

NATURAL ANTIMUTAGENIC AGENTS IN PHARMACOLOGY?

1 1 1 1
AHMET UYSAL , RAMAZAN CEYLAN , GKHAN ZENGIN , ERDOAN GNE

ABSTRACT
Human environment consists mutagenic and carcinogenic agents. Moreover, several well-
known mutagenic risk factors are closely connected with a modern lifestyle and their entire eradication
appears to be very burdensome, even unattainable. Therefore, there exists a need to reduce genotoxic
effects of mutagenic and carcinogenic factors by the regular intake of antimutagenic agents. Recently,
it has been accepted that plants and their products represent one of the main sources for compounds
with antimutagenic potential and, indeed, several secondary plant metabolites have demonstrated
chemo-preventive activities. Thus, there is an increasing interest in the investigation of naturally
occurring antimutagens from plants. So, Lathyrus czeczottianus methanol and water extracts were
evaluated for their potential mutagenic and antimutagenic activities by Ames test in the present study.
Salmonella typhimurium TA98 and TA100 strains used for determining the frame shift and base pair
exchange type of mutations, respectively. The Ames test was conducted both in the presence and
absence of metabolic activation enzymes. The result of the mutagenicity test showed that methanol
and water extract of Lathyrus did not increase the revertant colony numbers when compared with
control plates. We concluded that plant extracts were not mutagenic in the Ames test. Because of these
extracts were not mutagenic, so antimutagenic potentials of them were studied at doses of 10000
g/plate, 5000 g/plate, and 1000 g/plate. For TA98 methanol extract was moderate antimutagenic
(37%, 30%, respectively) at doses of 10000 and 5000 g/plate, while water extract showed moderate
antimutagenicity (33%) at the highest concentration. After addition of S9 mix, methanol extract
revealed strong antimutagenicity (73%, 63%, 57%) at all test doses against 2-aminoflourene. Similarly
water extract exhibited the greatest antimutagenic potential (85%) at a dose of 10000 g/plate with S9
mix for TA98 and followed by 5000 g/plate dose with 41% inhibition making the extract a very
strong antimutagen. Associated with sodium azide, methanol and water extracts were moderate
antimutagenic at a dose of 10000 g/plate in the absence of S9 enzymes for TA100 strain. The
methanol extract manifested strong antimutagenicity at concentrations of 10000 (78%) and 5000
g/plate (54%), and it alleviated the mutagenic action of 2-aminoanthracene in the presence of
metabolic activation system. It was observed that metabolic activation enzymes were increased the
antimutagenicity of the extracts and it can suggested that Lathyrus extracts may be the natural source
of antimutagenic agents against well-known mutagenic substances.

KEYWORDS
Antimutagenicity, Mutagenicity, Lathyrus czeczottianus, Ames test

Corresponding Author: AHMET UYSAL, ahuysal@selcuk.edu.tr

1
SELUK NVERSTES

1320
 Poster Session 10
Submission ID: 1272

COMPARISION OF THE FLAXSEED OILS OBTAINED BY USING

COLD PRESS AND SOLVENT EXTRACTION METHODS

1 1 1 1 1 2
ZIYA GEVREK , OSMAN KOLA , EYMA NCE , NURTEN CENGZ , MURAT REIS AKKAYA , TUNAHAN ERDEM

ABSTRACT
Flax (also known as common flax or linseed), Linum usitatissimum, is a member of the genus
Linum in the family Linaceae. Flaxseed oil is widely used in food industry due to its high content of
unsaturated fatty acids. In this study, oil was obtained by cold pressing and solvent extraction
methods. Cold pressing is completely a mechanical process without chemical application. In cold
pressing, the highest temperature which the product is exposed to, is 40C. During cold pressing,
flavor, aroma, nutrients and natural values of the plant are not lost. In the solvent extraction, solvents
are used to extract oil from seeds. In the present work, oils extracted by two different methods, cold
pressing and solvent extraction, were compared by analyzing several quality parameters of the oils
such as free fatty acid composition, antioxidant capacity, total phenolic content, peroxide value and
refractive index. Protein content of the flaxseed was found to be 14.5% While the yield of the cold
pressing was 21%, and that of solvent extraction was 34.8%. Antioxidant capacity was determined as
2.126 mmol TE/L for the oil extracted by the cold pressing and 2.144 mmol TE/L for the oil extracted
by solvent extraction. Total fenolic contents of the oil obtained by both cold pressing and solvent
extraction were 132.42 mgGA/L and 153 mgGA/L, respectively. There was not a significant
difference between the refractive indices of the oils extracted by both methods (determined as 1.48nd).
There was no active oxygen in the oil because it was stored in a dark refrigerator at 3C. According to
the characterization of fatty acids, the oils obtained by cold pressing contained 9.98% SFA, 19.81%
MUFA and 68.99% PUFA. Majority of these were -Linolenic acid (54.91%), oleic acid (17.65%),
linoleic acid (13.67%), palmitic acid (5.47%) and stearic acid (4.15%). SFA, MUFA and PUFA
contents of the oils extracted by solvent extraction were 10.77%, 18.06% and 70.30% PUFA,
respectively. Majority of the oils extracted by solvent extraction were -Linolenic acid (54.08%), oleic
acid (17.75%), linoleic acid (15.09%), palmitic acid (5.65%) and stearic acid (4.57%). This study
shows that flaxseed oil is a health-promoting ingredient for food industry because it contains
significant amounts of unsaturated fatty acids (especially -Linolenic acid) and protein.

KEYWORDS
Flaxseed, Fatty acids, Oilseeds, Phenolic compound, Protein content

Corresponding Author: ZYA GEVREK, ziyagevrek@gmail.com

1
ADANA BLM VE TEKNOLOJ NVERSTES
2
UKUROVA NVERSTES

1321
 Poster Session 10
Submission ID: 1273

ESSENTIAL OILS AS NATURAL PRESERVATIVES IN MEAT AND

MEAT PRODUCTS

1 1 1
SONGL AHIN ERCAN , HSEYIN BOZKURT , IDEM SOYSAL

D
ABSTRACT
Abstract Essential oils are aromatic and complex mixture of volatile compounds and can be

TE
obtained from different parts of plants: leaves, peels, roots, flowers, seeds, fruits and woods. Essential
oils are substantiated to have antiviral, antimycotic, antiparasitic, antioxidant, and insecticidal
properties in addition to the antibacterial action due to the phenolic functional groups. Production of
safe and high quality meat and meat products is a growing thought over the worldwide. There has been
increasing trend in essential oils as natural food preservatives in meat and meat products due to the
EN
concern about negative consumer perception of synthetic preservatives. Use of synthetic food
preservatives can cause adverse health effects and remained a challenge to the meat industry. Unlike
synthetic compounds, essential oils are rich in phenolics and they can enhance the overall quality of
food by decreasing lipid oxidation, microbial growth and overall acceptability . This review is covers
ES
up to date literatures on essential oils used as natural preservatives in meat and meat products.

KEYWORDS
PR

Essential oils, meat, meat products , preservatives.

T
O
N

Corresponding Author: SONGL AHN ERCAN, sosahin@gantep.edu.tr

1
DEPARTMENT OF FOOD ENGNEERNG, FACULTY OF ENGNEERNG, GAZANTEP UNVERSTY,
GAZANTEP 27310, TURKEY

1322
 Poster Session 10
Submission ID: 1275

DETERMINATION OF TEA CONSUMPTION VARIETY AND

FREQUENCY OF PEOPLE LIVING IN AKEHIR

1 1 1 1 1
ELMAS ERSZ , ALI EMRAH BIYIKLI , EZGI TOPTA BIYIKLI , FATMA EYDA KUTLUGN , MBERA IL , FATMA
1 1 1
GEN , ZAHIDE TUNCER , SINAN SIREN

ABSTRACT
Introducton Since time immemorial, tea is consumed frequently as curative and daily liquid
intake. Black tea is one of the common tea type, hence it is the most consumed tea in Turkey.
Preferability of tea is increased by people, as its positive effects on health issue became more topical.
Herbal teas consumptionability and conservationability is practical, therefore its consumption rate has
increased day by day. Objectve In this study, the aim is to determine the tea consumption rate and
frequency of people who live in Akehir town center. Method The study is made between February
2017 and March 2017. 320 people randomly selected for the study as 265(82.8%) women and
55(17.2%) men. By the survey research, participants demographic informations and habits related
with tea consumption are investigated. In order to analyze the data collected, SPSS 22.0 statistic
program is used. Results Age average of the people attended to the survey is 29.65.6. When the
education level of the people is investigated, the highest percentage belongs to high school graduates
by 82.2%. When the tea consumption rates in the survey are respectively sorted from high rate to low
rate; black tea(84.4%), linden tea(44.7%), green tea(39.7%), sage tea(33.2%), rose hip tea(29.1%),
mint tea(20.0%), chamomile tea(18.1%), apple tea(13.4%) and thyme tea(11.3%). Other teas
consumptions rates are found below 10%. 75.6% of the people who consume black tea prefers bulk
tea, 24.4% prefers tea bag. 32.8% of these people drink minimum 3 tea glass, 44.4% drink minimum 1
tea glass of black tea. 17.1% drink minimum 1 tea glass and 80.3% use green tea bag and 19.7% use
bulk green tea of people who consume green tea. Along the people who consume linden tea, 11.1%
drink minimum 1 tea glass and 69% of them use bulk linden tea and 30.7% use linden tea bag.
Conclusion Black tea consumption is the top consumed tea type as like people who live in all around
Turkey. The consumption rates of the other tea types after black tea are respectively, linden tea, green
tea and sage tea. Tea has lots of positive effects on health, especially due to its polyphenol content.
However, too much consumption of tea is not suggested, because it increases caffeine intake and has
diuretic effects. When consuming herbal teas, attention should be paid to the consumption amounts
according to the tea types.

KEYWORDS
Black tea, linden tea, green tea, herbal tea

Corresponding Author: ELMAS ERSZ, elmasonde@gmail.com

1
SELUK NVERSTES AKEHR KADR YALLAGZ SALIK YKSEKOKULU BESLENME VE DYETETK
BLM

1323
 Poster Session 10
Submission ID: 1276

HEALTH BENEFITS OF RESVERATROL; A PLANT PHYTOALEXIN

IN GRAPES AS A FUNCTIONAL FOOD

1 1 1 1
ALI SABIR , FERHAN SABIR , ZEKI KARA , ALPER KKBASMACI

ABSTRACT
Functional foods, derived from plants that naturally contain compounds beneficial to human
health (Boue et al. 2009), have been a developing area of food science research for the past decade
since consumer interest has shifted toward achieving and maintaining good health. Studies on
cardioprotective potential of dietary components supports the development of functional foods and
nutraceuticals. Plant phytoalexins as nutritional components has opened up a new area of food science.
On this point, resveratrol (3,5,4-trihydroxy-trans-stilbene), a natural phenol found in grapes, has a
calorierestriction effect and influences energy metabolism by improving insulin sensitivity, lowering
plasma glucose, and increasing mitochondrial (ORiordan et al. 2009). Experiments revealed that this
stilbenoid exerts antibacterial, antioxidant, anticancer and antiproliferative effects (Jang et al. 1997). It
has also been shown to extend the life span of several short-living species of animals (Valenzano et al.
2006). Recent studies also revealed that resveratrol has curative effect for Alzheimers disease.
Resveratrol is necessarily digested after ingestion and diffuses into blood rapidly. It maintains the
active form in foods due to its high resistance to heat (Keskin et al. 2009). These studies on the health
benefits of resveratrol spurred consumer interest in grapes as a functional food. Resveratrol is present
at high concentrations in the skin of grapes, depending on the grape variety. Experimental studies have
demonstrated that elicitor treatment can increase resveratrol concentrations of postharvest grapes. The
ability of UV irradiation to induce the synthesis of the phytoalexin resveratrol was first reported by
using leaf disks and immature grape berries (Langcake and Pryce 1977). In fact, many agents have
been proven to be effective for increasing the resveratrol content of grapes. Thus, recent studies
propose a new area within functional food research called phytoalexin-enriched foods that utilize
induced plant compounds or phytoalexins created either pre- or postharvest that have been considered
in terms of beneficial health effects.

KEYWORDS
Functional foods, grapes, resveratrol, antioxidant, health

Corresponding Author: AL SABIR, asabir@selcuk.edu.tr

1
SELCUK UNVERSTY, AGRCULTURE FACULTY, HORTCULTURE DEPARTMENT, KONYA, TURKEY

1324
 Poster Session 10
Submission ID: 1277

ANTIMICROBIAL EFFECTS OF ESSENTIAL OILS

1
MUSA ACAR

ABSTRACT
Plant essential oils have been used for many purposes since many years, especially in
scientific and commercial areas. At the beginning of these usage areas are cosmetics, medicine, food
industry, aromatherapy and phytotherapy. Essential oils have recently attracted the attention of many
scientists, because it has a wide use area, and the chemical structures of these essential oils have been
a subject of curiosity about the investigated biological activities. Essential oils are obtained from
various organs such as flowers, leaves, bark, branches or fruits of some plants and often referred to by
the name of the plant. These substances, called essences, are essentially terpene blends. Water drifted
with steam, insoluble in water, easily soluble in organic solvents. Essantial oils are produced by the
pressurization of plant parts by means of organic solvents, water vapor distillation or extraction. In
recent years, the insufficiency of synthetic drugs and the detection of side effects against increasing
diseases have increased the necessity of using natural products. For this purpose many plants are being
investigated from microbiological and pharmacological aspects. Since essential oils are complex
mixtures containing different compounds, they also differ in their biological effects. The effects are
different according to the substances, many essantial oils, have antimicrobial, carminative, coloretic,
sedative, diuretic, antispasmodic effects. Due to the increase in antibiotic-resistant infections in recent
years, studies on the search for new drugs in combating these infections are a great necessity. In this
regard, the plant essential oils have a great precaution and have been reported by many researchers as
antimicrobial agents. As in all the countries of the world, plants that are important in Turkey for
medical purposes have been used for centuries as a treatment for diseases among the people. As a
potential source of new antimicrobial compounds of these plants, which are traditionally envied, it is
very important to investigate scientifically.

KEYWORDS
Essential oil, herbal, antimicrobial

Corresponding Author: MUSA ACAR, musaacar@konya.edu.tr

1
NECMETTN ERBAKAN NVERSTES

1325
 Poster Session 10
Submission ID: 1278

USAGE AND ECONOMIC IMPORTANCE OF MEDICINAL AND

AROMATIC PLANTS IN THE LAST FIVE YEARS

1 1
TUBA AKDENZ FIRAT , ZUHAL SATI

ABSTRACT
With the developing world, the importance of medical plants is increasing considerably.
According to World Health Organization (WHO) researches, the number of plants used for therapeutic
purposes is around 20.000 and 80% of the population living in developing countries rely on traditional
herbal medicines for essential health needs. A lot of drug, still used in medicine, are made of plants.
For herbal product in intended property, quality medicinal plant is required. Turkey is one of the
important countries where medicinal plants grow with its geographical position, climate, vegetation
cover, agricultural potential for medical plants grow. Turkey has a lot of share in the trade of
medicinal and aromatic plants because it has many aromatic plants in its flora. Since ancient times,
these medicinal plants grown in our country have been benefited from various areas such as medicine,
food, cosmetics. The current increase in the popularity of medicinal plants results from resistance
developed by diseases. Extracts made from medicinal plants are effective against new breeds. For this
reason, there has been a serious return to herbal preparations in recent years. In this study, the last five
years and economic importance of medicinal and aromatic plants have been investigated because of
their increasing value.

KEYWORDS
medicinal and aromatical plants, economy, herbal extract

Corresponding Author: TUBA AKDENZ FIRAT, tugba.akdenizfirat@tarim.gov.tr

1
ZRA MCADELE MERKEZ ARATIRMA ENSTTS

1326
 Poster Session 10
Submission ID: 1279

DETERMINATION OF FATTY ACIDS AND ELEMENTS IN

CYCLOTRICHIUM NIVEUM (BOISS.) MANDEN. ET SCHENG.

1 2 1
AHMET ZKAYA , AHMET ZAFER TEL , UMUT YILMAZ HAN

ABSTRACT
Cyclotrichium genus is a member of the Lamiaceae family and its six species present in
Turkey. C. niveum and C. origanifolium and C. longiflorum are endemic for Turkey. C. niveum is
popularly known as dag nanesi (Gulcin et al., 2008). C. niveum is a perennial plant and is used in
the treatment of traditional pain, flu, nausea and irregular muscle pain. The plant, which has mint
flavor, is used in making soup, as a medicinal tea and as a spice in Turkish foods (etinus et al.,
2007). In recent years, the chemical composition of C. niveum has been studied. Pulegon and
isomenthol compounds are considered to be main ingredients in C. niveum. In addition, flavonoids
and triterpenoids are detected in other C. niveum species (Gulcin et al., 2008). Baser et al. (1994) have
reported essential oils of C. niveum for the first time (Baer et al., 1994). C. niveum has 32.5-56.1 g /
100 pulegon and 33.8-35.4 g / 100 isomenthol as the main ingredient in the essential oil (Gulcin et al.,
2008). The chemical components of C. niveum have been antioxidant effected (Alim et al., 2009). In
this study, we performed first literaturally known fatty acid and trace element analyses of the
C.niveum. C. nivenum plants were collected from Adiyaman/Nemrut mountain. After the plants were
picked up, they were naturally dried in a shady. The levels of trace element and fatty acid of C.
niveum endemic plant leaf in Turkey were determined by using ICP-OES and GC, respectively.
Among fatty acid levels, caprylic acid, undecanoic acid, palmitic acid, stearic acid, oleic acid, linoleic
acid and alfa-linolenic acid levels were determined as 11.19%, 10.27%, 14.01%, 3.58%, 8.14%,
6.54%and 15.69%, respectively. The plant leafs were found containing Ni, Pb, Zn, Cr, Ba, Al, Cu, Fe,
and Mn with respective quantities of 3.00, 0.07, 30.54, 122.17, 66.90, 319.98, 5.60, 334.52, 72.72
g/g, and K, Na at 47.47, 0.09 mg/g (dry matter) quantities, respectively. For the following medicinal
studies, we think that the obtained result parameters may be valuabled. REFERENCES [1] Gulcin, I.,
Tel, A. Z., Kirecci, E., (2008). Antioxidant, antimicrobial, antifungal, and antiradical activities of
Cyclotrichium Niveum (BOISS.) Manden and Scheng, International Journal of Food Properties, 11:
450471.RC Patra, D Swarup, SK Dwivedi, Toxicol., 162(2):8188 (2001). [2] etinus, .A., Gze,
., Sara, B., Vural, N., (2007). Scavenging effect and antispasmodic activity of the essential oil of
Cyclotrichium niveum, Fitoterapia, 78:129-133. [3] Baser, K.H.C., Sarikardagoglu, S., Tmen, G.,
(1994). The essential oil of Cyclotrichium niveum (Boiss.) Manden & Scheng, Journal of Essential Oil
Research, 6:9-12. [4] Alim, A., Goze, I., Cetin, A., Atas, A.D., Vural, N., Donmez, E., (2009).
Antimicrobial activity of the essential oil of Cyclotrichium niveum (Boiss.) Manden. Et Scheng,
African Journal of Microbiology Research, 3 8:422-425. Acknowledgments: This research was
supported by the ADYUBAP project, number FEFYL 2012/0011.

Corresponding Author: AHMET ZKAYA, aozkaya@adiyaman.edu.tr

1
DEPARTMENT OF CHEMSTRY, FACULTY OF SCENCE AND ARTS, ADIYAMAN UNVERSTY,
2
DEPARTMENT OF BOLOGY, FACULTY OF ARTS AND SCENCES, ADYAMAN UNVERSTY,

1327
KEYWORDS
Cyclotrichium niveum, element, fatty acid

1328
 Poster Session 10
Submission ID: 1280

API-AROMATHERAPY AND HEALTH

1 1
BANU YUCEL , ZUMRUT ACIKGOZ

ABSTRACT
Api-Aromatherapy is a mixture therapy by using combination of bee products and pure
essential oils. The properties of bee products mantain health for our body. Honey has antibacterial,
inflammatory, immunomodulator, antioxidant, expectorant, emollient effects. It cleanse the blood,
heals, remineralize the body and nutritious whereas propolis is cured several illnesses with its
excellent immunomodulatory, antibiotic, antiviral, antifungal, antiparasitic, antioxidant, anti-
inflammatory, analgesic, epithelisant, healing, prevents and treats periodontitis, depurativ and
haemorrhage attributes. Pollen is general stimulant of the body, energizing, antioxidant, rebalances the
intestinal flora, food liver, regulates body weight, remineralizant, antiallergic, negative effects of
excessive stress, antigeriatric. Royal jelly is stimulant of stem cells, boosts immunity, increase
performance and endurance, reduce fatigue, antibacterial, antiviral, restores skin and mucous
membranes, prevents early aging, hormonal regulator, maintain normal brain function. Essential oils
are aromatic volatile liquids have antibacterial, antiviral, antifungal, antiparasitic, antioxidant,
immunomodulator/immunostimulant, regenerator, inflammatory and antispasmodic, analgesic,
anesthetic, antiarrhythmic, anticoagulant hemostatic, cardiotonic, flebotonic,regulating endocrine
limfotonic hepatoprotective (gonads, thyroid, adrenal,pancreas), tonic, stimulant (nervous and
muscular) sedative, anxiolytic properties. Thyme (Thymus vulgaris), lavender (Lavandula
angustifolia), lemon (Citrus limon), mint (Mentha piperita), eucalyptus (Eucalyptus globulus),
chamomile (Matricaria chamomilla) and rose (Rosa arvensis) essential oils are use in Api-
aromatherapy application with bee products, mostly. Administration of bee products in combination
with essential oils could be used with internal and external (intranasal, enemas, masks dermo-
cosmetics, compresses healing, intravaginal, gingival) applications. In general using of internal
application, honey and water combined with essential oils for respiratory infections, insomnia,
depression, anxiety problems. Using in external, essential oils combined with honey, propolis or
pollen and then compressed or creamed. Combination of bee products and essential oils increase
immunity system with their synergic effects. So, mixing of bee products with essential oils, improve
the bioavailability of them more higher than using separately. The conglomeration of bee products and
essential oils (Api-aromatherapy), gives our bodies the essential balanced nutrition for an over all
physical and psychological well being

KEYWORDS
Bee products, health, aromatherapy, essential oils

Corresponding Author: ZUMRUT ACKGOZ, zumrut.acikgoz@ege.edu.tr

1
EGE UNVERSTY FACULTY OF AGRCULTURE DEPARTMENT OF ANMAL SCENCE

1329
 Poster Session 10
Submission ID: 1281

SEVERAL PROPERTIES OF OILS EXTRACTED FROM TEREBINTH

BY COLD PRESSING AND SOLVENT EXTRACTION

1 1 1 1 1 1
EYMA NCE , OSMAN KOLA , ZIYA GEVREK , NURTEN CENGZ , MURAT REIS AKKAYA , HAIM KELEBEK , ALI
1
EMRAH ETN

ABSTRACT
Medicinal and aromatic plants are the botanical sources used for, maintaining human health,
prevention and treatment of diseases. People have tried to cure diseases using plants or centuries.
Because of the success in these attempts, the use of plants in treatment of disease has continued up to
date. These medicinal plants are not only be used in medicine but they are also employed in nutrition,
beverage and cosmetic industries, organic farming and animal breeding. Among 350,000 identified
species, 35,000 are used for medicinal purposes. Only 5 % of these are chemically characterized.
Turkey is the gene pool of several plants and it includes several geographical regions where endemic
species grow. There has been special interest for the oils extracted by cold-pressing by consumers for
the last decades. Oils which are generally used in pharmaceutical and cosmetic industries has now
started to be consumed in our meals. Since oils extracted by cold pressing are not exposed to high
temperatures (maximum 40-50 C), solvents are not employed for oil extraction and higher amounts of
natural antioxidants, phosphatides, cerebrosides, carotenoids and phytosterols which are partly lost due
to refining in conventional oil production are present in cold-pressed oils, these oils has received
special interest in term of nutritional properties. Trebinth, a most-widely grown wild type of Pistachia
in Turkey, is a good candidate for consumption as food according to its properties. Among these
properties the oil content of trebinth is the most important. In this study, oils from terebinth were first
extracted from trebinth seed by cold-pressing and solvent extraction, several properties of extracted
oils were then characterized. Saturated and unsaturated fatty acid ratios were determined by analyzing
fatty acid compositions of the oils. The crude oil yield of terebinth was found to range between 30 and
35 %. Both protein and moisture contents of terebinth were determined as 5 % in average. Saturated,
mono-unsaturated and poly-unsaturated fatty acid contents of terebinth oil obtained by cold pressing
were 23.43, 38.08 and 28.26 %, respectively. Oil extracted by solvent extraction however had 9.99 %
saturated, 23.81 % mono-unsaturated and 52.93 % poly-unsaturated fatty acids. Essential fatty acids in
both oils obtained by cold pressing and solvent extraction were oleic (37.6 and 52.5 %, respectively),
palmitic (21.1 and 20.4 %, respectively) and linoleic (26.9 and 15.5 %, respectively) acids.
Additionally, free fatty acids, color, refractive index values were measured, antioxidant capacity and
total phenolic contents of the oil were determined by DPPH and Folin methods. Total phenolic
contents of oils extracted by cold-pressing and solvent extration were found to be 289.7 mgGA/L and
244.1 mgGA/L in average while total antioxidant capacity of respective oils were determined as 653.5
mol TE/L and 547.3 mol TE/L, respectively.

KEYWORDS

Corresponding Author: EYMA NCE, seymainc@gmail.com

1
ADANA BLM VE TEKNOLOJ NVERSTES

1330
Medicinal Aromatic Plant, Terebinth, Fatty Acid, Cold Pressing

1331
 Poster Session 10
Submission ID: 1282

THE USE OF AROMATIC PLANT OILS IN THE PROCESSING OF

TROUT EGGS AND THEIR EFFECT ON SENSORY PREFERENCES

1 2
BIBI AAISHAH OLLEE , ZKAN ZDEN

ABSTRACT
Todays consumers are more knowledgeable and careful about their health; this is why they
avoid synthetic food additives (Mastromatteo et al., 2011; Holley and Patel, 2005). Therefore to
reduce food borne pathogens new ways to reduce food borne illness must be put into practice, which
can be used together with other hurdle technologies (Burt, 2004). Caviar can undergo pasteurisation
but their taste is not well appreciated by consumers. Also, the salt concentration and storage
temperatures are not always efficient to preserve caviar (Fioretto et al., 2005). The use of essential oils
for food preservation has triggered a lot of interest (Holley and Patel, 2005). They are Generally
Regarded as Safe (GRAS) and are good alternative to synthetic antibiotics (El et al., 2014). Essential
oils have antimicrobial properties and can prolong the shelf-life of seafood alone or when used
together with other processing techniques (Mejlholm and Dalgaard, 2002) and can inhibit food borne
pathogens and spoilage microorganisms (Baydar et al., 2004). The fish roes were treated with thyme
oil and lemon oil. To the control group, no essential oil was applied. Panelists were asked to evaluate
the appearance, taste, texture and odour of the eggs. Out of the three groups, most of the panellists
preferred the group which was treated with lemon oil. Using essential oils together with fish roe, both
of which have functional properties ensures that the product is of the best quality and also might
provide more benefits for human health by preventing some diseases. Furthermore, the taste of the
product might also be enhanced and this might also create a new value added product.

KEYWORDS
Aromatic Plants Oil, Trout eegs, Processing, Sensory

Corresponding Author: BB AASHAH OLLEE, aaish0703@gmail.com

1
STANBUL NVERSTES FEN BLMLER ENSTTS
2
STANBUL NVERSTES SU RNLER FAKLTES

1332
 Poster Session 10
Submission ID: 1283

THE USE OF ESSENTIAL OILS IN INCUBATOR DISINFECTION

1 1 1
LKIN YCEL ENGN , BERNA ZTRK , EYDA ENTRK

ABSTRACT
The aim of this study was to develop a novel disinfectant derived from natural sources. The
formulation was prepared by combining equal portions of oregano, cinnamon and clove oils, which
has fresh odor and previously confirmed antimicrobial properties. The solutions prepared at different
concentrations (1%, 2%, 3%, 4% and 6%) were sprayed in incubators. To detect the microbial density
of incubator air, settle plates (30 min exposure) and air sampler (flow rate: 100 L/min) were used
together. Air sampling was performed before and 2 h after disinfection. Prior to disinfection, the total
mesophilic aerobic bacteria counts of incubator air were ranged from 35-43 CFU/plate for plates
sampled by air sampler and 13-28 CFU/plate for settle plates. Mold and yeast counts of incubator air
sampled by settle plates and air sampler were ranged between 3-8 CFU/plate and 1.5-5.5 CFU/plate,
respectively. No growth was observed on mold and yeast plates inoculated after disinfection
application in incubators. On the other hand, the formulation was found less effective on the total
mesophilic aerobic bacteria count (maximum reduction of 82.9%) than mold and yeast count. The
results indicated that the combination of essential oils could be effectively used in reducing the
microbial loads of incubator air. This application could be especially useful to prevent the mold
contaminations and also applicable for room disinfection.

KEYWORDS
incubator, air, disinfection, essential oil, air sampler

Corresponding Author: LKN YCEL ENGN, ilkinyucel@yahoo.com

1
EGE NVERSTES

1333
 Poster Session 10
Submission ID: 1284

USING MEDICINAL AND AROMATIC PLANTS IN FUNCTIONAL

EGG PRODUCTION

1 1
ZUMRUT ACIKGOZ , BANU YUCEL

ABSTRACT
Hens egg is a cheap, healthy and nutritious conventional food supplying several essential
nutrients, excellent protein quality and low energy content. In addition, egg contains some biologically
active compounds (lysozyme, ovalbumin, ovomucoid, ovomucin, ovotransferrin, cystatin, avidin,
phosvitin etc.) which might have a role in the therapy and prevention of chronic and infectious
diseases. In many scientific reports, it was indicated that these compounds has antimicrobial,
immunomodulator, bone growth promoting, antioxidant, anticancer and antihypertensive properties.
Taking into account the presence of all these components, the use of egg is becoming increasingly
widespread in cosmetic, pharmacology, medicine, biotechnology, nutraceutical. Egg has been
described (defined) as a Natures original functional food because of providing a number of
benefical nutrients to sustain life and growth. Nevertheless, in recent years, egg industry is developing
new functional eggs to meet the growing demands of health conscious consumers. In these eggs, some
of biological active compounds are enhanced by dietary manuplation. Eggs enriched with n-3 fatty
acids, antioxidants, carotenoids and minerals have attracted a lot of attention in nutritional sciences.
The content of n-3 fatty acids in eggs could be increased by adding fish meal/oil, flaxseed (linseed),
canola (rapeseed) and marine algae to diet. In egg enhanced with n-3 fatty acids, oxidavite sensitivity
has increased and shelf life has been shortened. Thus, the antioxidant status should also be subjected in
eggs altered fatty acid profile by dietary manuplation. Currently, natural antioxidants are in high
demand because of their health-enhancing and disease-risk-preventing properties. Globally, medicinal
and aromatic plants or their extracts has been supplemented to diets for improving the antioxidant
capacity of eggs. In recent years, several in vitro and in vivo studies have been conducted to determine
the antioxidant effects of aromatic plants and spices, especially in cinnamon, cumin, laurel, mint,
oregano, rosemary and sage. In this review, it would be given information about medicinal and
aromatic plants or their extracts using in functional egg production.

KEYWORDS
Functional egg, medicinal and aromatic plant, antioxidant.

Corresponding Author: ZUMRUT ACKGOZ, zumrut.acikgoz@ege.edu.tr

1
EGE UNVERSTY FACULTY OF AGRCULTURE DEPARTMENT OF ANMAL SCENCE

1334
 Poster Session 10
Submission ID: 1286

SEVERAL PROPERTIES OF OILS EXTRACTED FROM TEA SEEDS

BY COLD PRESSING AND SOLVENT EXTRACTION

1 1 1 1 1 1
OSMAN KOLA , EYMA NCE , ZIYA GEVREK , HAIM KELEBEK , NURTEN CENGZ , MURAT REIS AKKAYA , ALI
1
EMRAH ETN

ABSTRACT
Tea (Thea sinensis) is a plant classified under the species sinensis of Thea genus in
Theaceae family. This plant, also known as Camellia sinensis by botanists. Although tea is native
to Southern and South-Eastern Asia, it can be planted in tropical and subtropical regions. The first
attempt in the plantation of tea in our country was made in the end of XIX. century. Inadequate
knowledge of climatic conditions needed for tea plantation resulted in the failure of this attempt
performed in Bursa. Later on, Rize was selected as a proper place for tea cultivation and first tea
plantation was established by the efforts of Zihni Derin, ex-general director of Agricultural Affairs,
using tea seeds and seedlings provided from Batum in 1924. The tea plantation, thus production of
processed tea in our country has increased since 1940. The color and taste of the steeped tea almost
results from the presence of secondary phenolic compounds. Two polyphenols; theaflavins and
thearubigins have significant contributions to the properties of steeped tea. Additionally, glycosides,
alkoloids, pectic substances and sugars are effective on the taste formation. The aroma of tea results
from resinoids, essential oils, carotenes and the decomposition products of aminoacids. A group of
compounds in lemon-yellow color and stringent in taste which can be easily solidifed and smelling
like tea produces the aroma of tea. Another active compound found in tea, especially in tea seed, is oil.
The oil found in tea seed has been reported to have antimicrobial, antithrombic, antispasmodic,
carminative properties and to be effective in the prevention of cardiovascular diseases. It is known that
tea seed oil has natural antioxidant activity and this is partly due to the presence of phenolic
compounds in higher amounts. Oils obtained by cold pressing receive significant attention since they
do not expose to higher temperatures (maximum 40-50 C) and solvents during the production and
they contain significant amounts of natural antioxidants, phosphatides, cerebrosides, carotenoids and
phytosterols which are partly lost during refining in conventional oil production. Oils from tea seeds
were extracted by cold pressing and solvent extraction and the several properties of these oils were
characterized in this study. Crude oil yield of tea seeds obtained from Rize were found to be % 15 -
20. Total phenolic contents of tea seed oil obtained by cold-pressing and solvent extraction were
determined as 125.3 mg GA/L and 219.1 mg GA/L. Total antioxidant capacities of tea seed oils
however were 270 mol TE/L and 489 mol TE/L for cold-pressing and solvent extraction,
respectively. Tea-seed oil extracted by cold-pressing were found to contain 19.3 % saturated fatty
acids, 54.7 % mono-unsaturated and 24.7 % poly-unsaturated fatty acids. Saturated, mono-unsaturated
and poly-unsaturated fatty acid contents of oils obtained by solvent extraction were determined as
19.1, 53.9 and 25 %, respectively. Essential fatty acids found in oils extrated from tea seeds by cold
pressing and solvent extraction were found to be oleic (53.3 and 52.8 %, respectively), linoleic (24.3
and 24 %, respectively) and palmitic (15.8 % for both) acids.

Corresponding Author: OSMAN KOLA, okola@adanabtu.edu.tr

1
ADANA BLM VE TEKNOLOJ NVERSTES

1335
KEYWORDS
Tea seed, Antioxidant, Fenolic compounds, Fatty Acids, Cold Pressing

1336
 Poster Session 10
Submission ID: 1287

TRACE ELEMENT AND FATTY ACID LEVELS OF FRITILLARIA

PINARDII BOISS. PLANT SEED AND BULB

1 2 1
AHMET ZKAYA , AHMET ZAFER TEL , UMUT YILMAZ HAN

ABSTRACT
Plants are important food sources for the people. Therefore, many studies are devoted to
quality and nutritional values of plant [1]. Fatty acids and trace elements are very important for human
life [2, 3]. The levels of fatty acid and trace element of Fritillaria pinardii BOISS seed and bulb in
Turkey were determined by using ICP-OES, and GC, respectively. In this study, we performed first
literaturally known fatty acid and trace element analyses of the Fritillaria pinardii. These plants were
collected from Adiyaman/Nemrut mountain. After the plants were picked up, they were naturally dried
in a shady. Among fatty acid levels of the plant bulb, at highest rates, was found containing 18.30%
palmitic acid, 15.01% stearic acid, 19.71% oleic acid, 9.23% eicosatrienoic acid and 8.74%
docosahexaenoic acid. The plant bulb was found containing Ni, Zn, Cr Ba, Al, Cu, Fe, and Mn, with
respective quantities of 1.17, 24.45, 1.89, 8.15, 5.94, 0.75, 8.57, 19.69 g/g, and K, Na at 19.56, 0.01
mg/g (dry matter) quantities, respectively. The fatty acids contained in the seed of that plant were
found to be 14.23% palmitic acid, 4.13% stearic acid and 17.01% oleic acid, 53.59% linoleic acid and
2.93% alfa-linolenic acid. In the seed of plant, the quantities of Ni, Zn, Cr, Ba, Al, Cu, Fe and Mn
were determined as 2.22, 27.26, 1.39, 15.27, 40.95, 3.47, 79.81, 32.62 g /g, respectively while the
quantities of K and Na levels were at 43.47, 0.07 mg/g (dry matter). From these results, it can be
important for the future medicinal studies. Key Words: Fritillaria pinardii, element, fatty acid
REFERENCES [1] H. Yeilaghi, A. Arzani, M. Ghaderian, R. Fotovat, M. Feizi, and S. S. Pourdad,
Effect of salinity on seed oil content and fatty acid composition of safflower (Carthamus tinctorius
L.) genotypes, Food Chemistry, vol. 130, no. 3, pp. 618625, 2012. [2] O. B. Citil, E. Tulukcu, and
A. Kocak, A comparative study of the fatty-acid composition of Sesamum indicum oil obtained from
different provinces in Turkey, Chemistry of Natural Compounds, vol. 47, no. 1, pp. 98100, 2011. [3]
T. Sabudak, Fatty acid composition of seed and leaf oils of pumpkin, walnut, almond, maize,
sunflower and melon, Chemistry of Natural Compounds, vol. 43, no. 4, pp. 465467, 2007.
Acknowledgments: This research was supported by the ADYUBAP project, number FEFYL
2012/0011.

KEYWORDS
Fritillaria pinardii, element, fatty acid

Corresponding Author: AHMET ZKAYA, aozkaya@adiyaman.edu.tr

1
DEPARTMENT OF CHEMSTRY, FACULTY OF SCENCE AND ARTS, ADIYAMAN UNVERSTY,
2
DEPARTMENT OF BOLOGY, FACULTY OF ARTS AND SCENCES, ADYAMAN UNVERSTY,

1337
 Poster Session 10
Submission ID: 1288

PHENOLIC COMPOUNDS CONTENT AND ANTIOXIDANT

CAPACITY IN FRUITS OF SOME EGGPLANT (SOLANUM
MELONGENA L.) CULTIVARS*

1 1 1 1 1
BELGIZAR KARAYIGIT , SEMA HAYIRLIOGLU-AYAZ , AYNUR KURT , NESRIN COLAK , FAIK AHMET AYAZ

ABSTRACT
Aim of the study: Eggplant (Solanum melongena L.) is one of the most commonly consumed
vegetable crops worldwide, and one with important beneficial effects on human health. These benefits
are mainly due to eggplants highly bioactive antioxidant constituents, such as phenolic compounds
including flavonoids and anthocyanins. The objective of this study was to determine and compare the
phenolic compound content and antioxidant capacity of seven commercial eggplant fruits in Turkey.
Materials and Methods: The seeds of seven common eggplant cultivars (Aydn siyah, Pala 49, Super
pala, Kemer 27, Kadife kemer, Topan, and Kadife) were grown in soil, and seedlings were obtained
from each cultivar using standard cultivation techniques for eggplant. The fruits were harvested when
they reached commercial market size. The fruit was treated with liquid nitrogen and stored at -80 C
until analysis. Standard methods were used to determine total phenolic compounds (TPC), flavonoid
(TF) and anthocyanin (TACY) contents and antioxidant capacity (DPPH, FRAP, and CUPRAC)
values. All results were expressed as dry weight (dw). Results: The TPC content (mg GA/100 g) in the
cvs. ranged from 1019.51 to 613.20 (average, 862.58), and the TF content (mg QE/100 g) from 244.76
to 184.13 (average, 225.86). Super pala had the highest TPC and TF contents (1019.51 and 244.76
mg/100 g, respectively). The highest TACY content was determined in Kemer 27 (221.02 mg/100 g)
eggplant. DPPH radical scavenging activity ranged from 54.30 to 37.31 mol TEAC/g, FRAP from
106.93 to 53.83 mol TEAC/g and CUPRAC from 126.11 to 50.57 Mol TEAC/g. Super pala had the
highest DPPH radical scavenging activity (54.30 mol TEAC/g), and FRAP (106.93 mol TEAC/g)
and CUPRAC (126.11 mol TEAC/g) levels. In conclusion, eggplant can be beneficial to human
health because of its high phenolic contents and antioxidant capacity values. *Acknowledgment:
Financial support for this study was provided by the Research Fund of Karadeniz Technical University
in Turkey (Project No: 2008.111.004.04 ).

KEYWORDS
Eggplant, Solanum melongena, Antioxidant, Phenolic, Anthocyanin

Corresponding Author: BELGZAR KARAYIGIT, belgizar.06@gmail.com

1
KARADENZ TEKNK NVERSTES

1338
 Poster Session 10
Submission ID: 1289

DEVELOPMENTS IN MEDICINAL AND AROMATIC PLANT

PRODUCTION IN TURKEY

1 1 1
SIBEL KADIOLU , BANU KADIOLU , GKHAN TAIN

ABSTRACT
Day by day attention to medicinal and aromatic plants increases causing rise in commerce and
production of these plants. Turkey has features of three old continents and flora of three
phytogeography. Turkeys flora includes many herbal products that constitute input for herbal
medicine, herbal chemicals, food and additives, cosmetics and fragrance industry of developed
countries. A significant part of rich plant diversity is used as medicinal and aromatic plants. Medicinal
and aromatic plants that are collected from the nature and cultured have a lot of economic potential.
Among agricultural plants, opium, cumin, anise, thyme and rose (for oil) take place on top. In
addition, it is not possible to reach the data for the plants that have small breading areas and
production amount. According to the data of TUIK, in Turkey although the breading area of medicinal
and aromatic plants (poppy (seed/capsule), lupin hop, melissa, nettle, sage, rose (for oil), lavender,
anise, cumin, thyme, blackcumin, fenugreek, fennel, coriander, heather, capers, dill, parsley, mint,
purslane, rocket, water cress, sweet potato ) was 93.633 ha in 2016 that is 2.401 ha less than the
previous year, production decreased by 3.809 tones. For all that an increase is seen in the last five
years. Poppy, cumin and thyme contributed a lot to the increase in the breading area and production
amount. Additionally, increase in blackcumin and fenugreek cannot be denied. When compared to
other countries it is seen that Turkey cannot benefit economically from rich medicinal and aromatic
plant diversity. For sustainable production of medicinal and aromatic plants, agriculture should
become widespread.

KEYWORDS
Medicinal and aromatic plant, collecting from nature, production, plant diversity

Corresponding Author: SBEL KADIOLU, sibel.kadioglu@hotmail.com

1
DOU ANADOLU TARIMSAL ARATIRMA ENSTTS MDRL

1339
 Poster Session 10
Submission ID: 1290

HYALURONIC ACID EXTRACTION FROM KOMBUCHA SYMBIOSIS

1 1 1 1
SIBEL BAYIL OUZKAN , BRAHIM HALIL KILI , SINA AKAN GMBURUN , SAMI SERHAT TOSUN , BEKIR
1
SIDDIK KURT

ABSTRACT
Kombucha is a kind of mushroom tea which can be used to alternative drug in folk remedies.
Kombucha was originated East Asian firstly and then coming to Russian. Kombucha mushroom is
living a solution of sugar and varieties organism and produce continously. Kombucha contains over 50
different kinds of probiotics, organic enzymes, amino acids and vitamins components. Especially,
kombucha contains hyaluronic acid and glucosamine which is why its so effective in relieving joint
pain. Hyaluronic acid is a linner polysaccharide which used to be cilinically drug and dermatology.
Previous studies were found to hyaluronic acid in kombucha symbiosis and generally using at
cosmetic industry effectively. In our laboratories we produced the kombucha mushroom suitable
fermatation and conditions so that we had so many kombucha teas for drinking and testing. In this
project, we will be try to solated from hyaluronic acid both of kombucha mushrooms and teas firstly.
After that we are going to purity of hyaluronic acid from kombucha samples and prepare the most
suitable extraction of hyaluronic acid. GC-MS and HPLC will be used for structure and quantities of
samples How the hyaluronic acid will be determined in kombucha samples then need to compare with
commercially hyaluronic acid. Also some biological parameters as DNA protection activity and
antmicrobial activities will be find from this extractions. We believe that this project is very important
for cosmetic and drug industry.

KEYWORDS
Kombucha, Hyaluronic acid, GC-MS, HPLC

Corresponding Author: SBEL BAYIL OUZKAN, gokayoguzkan@gmail.com

1
GAZANTEP NVERSTES

1340
 Poster Session 10
Submission ID: 1291

ESSENTIAL OIL COMPOSITION OF CHONDRILLA JUNCEA L.

FROM STANBUL

1 2
MEHMET ZDEMIR , HSEYIN SERVI

ABSTRACT
Chondrilla juncea of Asteraceae is an invasive plant species native to Europe, Asia and North
Africa. This plant finds edible use in the Mediterranean countries. However there is only few reports
on the chemistry of this species. Previously isolation of triterpene, phenolic and sesquiterpene
glycoside types of compounds were reported from C. juncea. To the best of our knowledge this is the
first report on the essential oil composition of C. juncea. Chondrilla juncea was collected during the
flowering period from stanbul. Essential oil was obtained with a clevenger apparatus by 3h
distillation. Yield of the essential oil was <0.01%. The GC-MS analysis of the essential oil was
performed with an Agilent 5977 MSD system operating in EI mode. Essential oil sample was diluted
1:10 (v:v) with n-hexane. Injector and MS transfer line temperatures were set at 300 and 250C
respectively. Splitless injection was employed. Innowax FSC column (60 m x 0.25 mm, 0.25 m film
thickness) and helium as carrier gas (1 mL/min) were used in GC-MS analyses. Oven temperature was
programmed to 60C for 10 min. and raised to 220C at rate of 4C/min. Temperature kept constant at
220C for 10 min. and then raised to 240C at a rate of 1C/min. Mass spectra were recorded at 70 eV
with the mass range m/z 35 to 425. Relative percentage amounts of the separated compounds were
calculated from integration of the peaks in MS chromatograms. Identification of essential oil
components were carried out by comparison of their relative retention indices (RRI) obtained by series
of n-alkanes (C5 to C30) to the literature and with mass spectra comparison. Thirty six compounds
were identified representing 87.5% of the essential oil of Chondrilla juncea. The main components of
Chondrilla juncea were identified as hexadecanoic acid 20.2%, heneicosane 18.2%, hexahydrofarnesyl
acetone 5.4%, octadecane 5.2%, heptacosane 4.5% and phytol 3.7%.

KEYWORDS
Chondrilla juncea, essential oil, hexadecanoic acid, heneicosane, hexahydrofarnesyl acetone

Corresponding Author: MEHMET ZDEMR, mehmetozdemir@ogm.gov.tr

1
TC ORMAN GENEL MDRL, MARMARA ORMANCILIK ARATIRMA ENSTTS MDRL
2
YILDIZ TEKNK NVERSTES, FEN BLMLER ENSTTS, ORGANK KMYA BLM

1341
 Poster Session 10
Submission ID: 1292

ESSENTIAL OILS OF SOME SPICES AND MEDICINAL PLANTS

1 1 1
ASLI ELKEL , M. SERDAR AKIN , K. BURCU BYKKILI

ABSTRACT
Essential oils are generally volatile and odourus mixture that are liquid form in normally,
which is obtained by, water, or water vapor distillation solvent extraction and pression method from
plants or some parts. Many substances of essential oils are widely has been used in the food,
pharmaceutical, paint, mining and cosmetic industries. Essential oils have anti-spasm, irritant,
antiseptic, mutagenic, antimutagenic and antimicrobial properties. There are also many researches
have been effects of essential oils against on the microorganisms which cause food spoilage and
poisoning. The composition and amount of essential oils are cahged depens plants type, plants part,
the region, the production place and production methods. Some of plant species widely produced in
the World are sage, carnation, saffron, turmeric, cinnamon, etc., and contained essential oil. About 500
of Sage species have been identified so far. These species are distributed in tropical and subtropical
regions. It contains essential oil components such -tuyo of sage, -tuyo of 1.8-cineol, camphor,
borneol and - pinene etc. Studies have shown that essential oils containing tuyen, camphor and
caryophile in sage (Salvia officinalis) have antifungal activity. It was determined that + tuyen, 1,8-
cineol, limonene and camphor materials ,which constitute the main components of Salvia officinalis L.
(sage) essential oil, did not show mutagenic properties when they used separately. But if they used
together, they would give positive results except antimutagenicity tests (except camphor) Cloves have
appetizing, digestive stimulant and antiseptic properties. Overall it have included essential oil
components of eugenol, caryophyllene, eugenyl acetate, humulrn, ylang and methoxybenzaldehyde.
Studies have shown that mixtures of cloves, thyme, mint and lemon essential oils reduce the number
of Clostridium perfringens. Saffron is a dried stigma of Crocus sativus L species. Its the most
important essential oil is safranal. Turmeric (Curcuma longa L. (saffron root, Indian saffron)) is
commonly used instead of saffron plant as a spice. The most important components of turmeric
essential oil are Turmeron, zingiberen, kurukumen, bizabolen, zingeron, atlaton, kurkumin, 1.8 cineol,
-felandren, caryophyllene, p-cymene and farnesen. It has been reported that the component of
curcumin essential oil obtained from Curcuma longa L plant had strong antioxidant activity.
Cinnamon is obtained from the dried shell of Cinnamomum (Lauraceae) type plant. Ibn-i Sina
reported in the book al-Knn f't-Tbb that the effects of cinnamon are refreshing and heating up and
eliminated the bad effects of diseases. Some important essential oil components found in cinnamon are
cinnamic aldehyde, eugenol, cinnamyl, acetate and hydrocyanic aldehyde. Mint (Menta) plant contains
significant amount of essential oil and main components of its essential oil are menthol, menthol,
menthofuran, menthyl acetate, -terpinene, - and -pinene, 1,8-cineole, limonene, linalool, ethyl
amylcarbinol, pulegone and piperidone. It has been reported that different types of mint have some
positive effects on humans such as antimicrobial, antispasmodic, coleretic, carminative.

Corresponding Author: ASLI ELKEL, acelikel2@gmail.com

1
HARRAN NVERSTES, ZRAAT FAKLTES, GIDA MHENDSL BLM, ANLIURFA

1342
KEYWORDS
Essential oils, Sage, Cinnamon, Mint, Clove.

1343
 Poster Session 10
Submission ID: 1293

"KANLICA MASHROOM" THAT A WILD EDIBLE MUSHROOM

VARIETIES FROM KASTAMONU: SPECIES, FUNCTIONAL
PROPERTIES AND ALTERNATIVE USES

1 1
RABIA ATASOY , MGE HENDEK ERTOP

ABSTRACT
Wild edible mushrooms have been collected and consumed by people for their nutritional
value and they are occasionally consumed for their supposed medicinal value. They are known as
highly valued non-wood forest products today, thus wild edible mushrooms have played an important
role in providing new sources of income in the whole World. Turkey has also very large variety of
wild edible mushroom, especially in Black Sea Region. Kanlca mushroom, also known as pine
mushroom and intar mushroom, is from Russulaceae family. It has Lactarius deliciosus, Lactarius
deterrimus,Lactarius salmonicolor and Lactarius semisanguifiluus species. It is seen in leafy tree
forests, pine forests and meadows in the world, after spring and autumn rains. It is grown in
Kastamonu, Sinop, Bursa and Balkesir in the interior parts of the West and Central Black Sea. It is
among the wild edible mushrooms and has an important place among the non-wood forest products in
Kastamonu. The mushroom has local consuming causes and consumption patterns around
Kastamonu.The vast majority of harvest and consumption takes place in August and November by the
local people from their natural environment. Moreover, conservation in saline water is quite
common.The direct consumption is not appropriate since it has a bitter taste.It is usually consumed
after boiling in water. The taste resembles meat. The mushroom boiling water is also used for facial
treatment for acne treatmentby the local people. Kanlca mushroom is a protein source, and rich in
amino acids, mineral substances and vitamins (B1, B2, C vitamins and niasin). Morphologically and
visually it is quite different from cultured mushrooms and many wild mushrooms. It has 5-15 cm
diameter and the middle part is concave.The mushroom hat is orange and yellow, the lamellas are
reddish yellow and white, and the handle is wine red and turmeric.They are characterized by the
unique ability to produce a red milky fluid, if cut or broken. The crumb of Kanlca mushroom is fruit-
smelling, soft, reddish yellow-white. In this study, the types of Kanlca mushroom in Kastamonu
region, food and non-food alternative consumption reasons and functional qualities have been
investigated.

KEYWORDS
Wild edible mushrooms, Kanlca mushroom, intar, pine mushroom, Kastamonu

Corresponding Author: MGE HENDEK ERTOP, mugeertop@kastamonu.edu.tr

1
KASTAMONU NVERSTES MHENDSLK VE MMARLIK FAKLTES GIDA MHENDSL BLM

1344
 Poster Session 10
Submission ID: 1294

PHENOLIC COMPOUNDS CONTENT AND ANTIOXIDANT

CAPACITY OF ENDEMIC TRIPLEUROSPERMUM BAYTOPIANUM
AND T. REPENS (ASTERACEAE) FROM TURKEY*

1 1 1 1 1
BELGIZAR KARAYIGIT , HUSEYIN INCEER , NESRIN COLAK , SEMA HAYIRLIOGLU-AYAZ , FAIK AHMET AYAZ

ABSTRACT
Aim of the study: Many edible plants play a protective role in the maintenance of human
health due to the antioxidant properties of their biologically active compounds known as plant
phenolics. They also contribute an important part of plant secondary compounds with a significant
impact on defense mechanisms in plants. The genus Triplepleurospermum (Asteraceae) is of
considerable medicinal value. Its phytocehmical profiles, mostly consisting of volatile/oil components,
exhibit a range of health benefits including anti-inflammatory, antiseptic, antifungal, antibacterial,
antiulcer and antioxidant activities. This study investigated the phenolic compounds content (TPC)
and antioxidant capacity (AC) of endemic T. baytopianum E. Hossain and T. repens (Freyn & Sint.)
Bornm. for the first time in the literature. Materials and Methods: T. baytopianum and T. repens
specimens were collected from different habitats in Turkey. The whole plant, including root, shoot and
leaf, was fully dried in the shade at room temperature and low humidity and stored until analysis. The
capitulum was separated and used in the extraction procedures. TPC and AC values assayed with
DPPH radical scavenging activity, ferric reducing antioxidant power (FRAP) and cupric reducing
antioxidant capacity (CUPRAC) were measured using standard methods published elsewhere. Results
were expressed as dry weight (dw) units. Results: TPC and AC values differed significantly between
the species. TPC content was 32.8 mg GA/g dw in caitulum in T. repens, higher than in T.
baytopianum (14.1 mg GA/g DW). AC values (mol TE/g dw, DPPH, CUPRAC, FRAP) also differed
between the species. T. repens exhibited higher AC values (4.2 DPPH, 12.0 FRAP and 3.7 CUPRAC)
than T. baytopianum (2.0, 8.1 and 2.8 , respectively). *Acknowledgments: The authors thank the
Scientific and Technological Research Council of Turkey (TUBITAK, project no: 102T162) for its
financial support.

KEYWORDS
Antioxidant, Tripleurospermum baytopianum, Tripleurospermum repens, Phenolics,
Antioxidant

Corresponding Author: BELGZAR KARAYIGIT, belgizar.06@gmail.com

1
KARADENZ TECHNCAL UNVERSTY

1345
 Poster Session 10
Submission ID: 1295

ESSENTIAL OIL COMPOSITION OF SCILLA AUTUMNALIS L.

FROM STANBUL

1 2
MESUT TANDOAN , HSEYIN SERVI

ABSTRACT
Previously essential oil composition of Scilla autumnalis was not reported. However there is
considerable amount of report on the chemistry of non-volatile secondary metabolites of Scilla
species. Until now homoflavonoids, stilbenoids, alkaloids and cardiac glycosides were isolated from
Scilla species. Scilla autumnalis was collected during the flowering period from stanbul. Essential oil
was obtained with a clevenger apparatus by 3h distillation. Yield of the essential oil was <0.01%. The
GC-MS analysis of the essential oil was performed with an Agilent 5977 MSD system operating in EI
mode. Essential oil sample was diluted 1:10 (v:v) with n-hexane. Injector and MS transfer line
temperatures were set at 300 and 250C respectively. Splitless injection was employed. Innowax FSC
column (60 m x 0.25 mm, 0.25 m film thickness) and helium as carrier gas (1 mL/min) were used in
GC-MS analyses. Oven temperature was programmed to 60C for 10 min. and raised to 220C at rate
of 4C/min. Temperature kept constant at 220C for 10 min. and then raised to 240C at a rate of
1C/min. Mass spectra were recorded at 70 eV with the mass range m/z 35 to 425. Relative percentage
amounts of the separated compounds were calculated from integration of the peaks in MS
chromatograms. Identification of essential oil components were carried out by comparison of their
relative retention indices (RRI) obtained by series of n-alkanes (C5 to C30) to the literature and with
mass spectra comparison. The GC-MS analysis of S. autumnalis essential oil presented the main
components as heptacosane 21.5%, pentacosane 12.5%, nonacosane 7.3%, hexadecanoic acid 4.0%,
hexahydrofarnesyl acetone 3.4% and hexadecanoic acid methyl ester 2.7%.

KEYWORDS
Scilla autumnalis, essential oil, heptacosane, pentacosane, nonacosane

Corresponding Author: MESUT TANDOAN, mesuttandogan@ogm.gov.tr

1
TC ORMAN GENEL MDRL, MARMARA ORMANCILIK ARATIRMA ENSTTS MDRL
2
YILDIZ TEKNK NVERSTES, FEN BLMLER ENSTTS, ORGANK KMYA BLM

1346
 Poster Session 10
Submission ID: 1296

CORIANDER SEED OIL: THE BENEFITS ON SKIN REGENERATION

1 1
AHSEN SEVDE INAR , ALEV NDER

ABSTRACT
Coriandrum sativum L. belongs to the Apiaceae family, but its origins are still indefinite. The
Coriander genus was represented in Flora of Turkey with two different species as C. sativum and C.
tordylium. The major component was determined as linalool (% 60-70) in the essential oil of the
coriander. It also contains fatty acids, coumarins, flavonoids, polyphenols in the extracts. Moreover, it
is known as a medicinal plant used in many disorders. The coriander has been used in various fields
especially in food and pharmaceutical markets. This current study is concentrated on methods of
obtaining essential or fatty oils from coriander seeds for use in prescriptions. That is why, we believe
that in this presentation we will put forward the features of the skin regeneration, derive the methods
of obtaining and we will discover all the interesting effects of coriander, discover effects together with
formulations.

KEYWORDS
Apiaceae, Coriander, Oil, Coriandrum

Corresponding Author: AHSEN SEVDE INAR, cinarahsen@gmail.com

1
ANKARA NVERSTES, ECZACILIK FAKLTES

1347
 Poster Session 10
Submission ID: 1298

SUGARS AND ORGANIC ACIDS IN BERRIES OF ERZINCAN BLACK

GRAPE (VITIS VINIFER

1 1 1 2 1
AYNUR KURT , SEMA HAYIRLIOGLU-AYAZ , NESRIN COLAK , ERDAL AKPINAR , FAIK AHMET AYAZ

ABSTRACT
Aim of the study: Grapes (Vitis spp.) are one of the most important and nutritious horticultural
crops. They are often used in diets and are widely regarded as beneficial for human health and as
preventing various degenerative diseases due to their polyphenol content. Sugars and organic acids are
natural components of black grapes and play a significant role in determining nutritional value and
maintaining fruit quality, flavor and stability. The Erzincan black grape (Vitis vinifera Karaerik) is a
patented and certified agricultural product in Turkey. The aim of this study was to examine some
physicochemical (pH, TA, etc.) parameters and the sugars and organic acids in different parts of the
black grape, which grows naturally in Erzincan province, Turkey. Materials and Methods: Grape berry
samples, at commercial maturity stage randomly harvested from different locations in Erzincan, were
immediately frozen in liquid nitrogen. Sugars and organic acids in the grape berry parts were separated
and quantified using HPLC and the physicochemical parameters measured by using standard
published methods (pH, TA, MC, DM, FD, etc.) All results were expressed as fresh weight (fw).
Results: The pH values and TA contents in berries of the black grape were 3.50 and 0.59 g citric
acid/100 g, respectively. The berry diameter (FD, 20.17%), moisture content (MC, 81.06%) and dry
matter (DM, 17.51%) were also determined. Fructose (peel, whole grape and seed; 188.86, 126.05,
and 13.69 g/kg fw, respectively) and glucose (peel, whole grape and seed; 152.6, 105.6, and 9.9 g/kg
fw, respectively) were the most abundant sugars, and tartaric acid (peel, whole grape and seed; 6.78,
2.81, and 1.98 g/kg fw, respectively) and malic acid (peel, whole grape and seed; 2.6, 2.6, and 6.87
g/kg fw, respectively) were the major organic acids identified. In conclusion, the black grape contains
high levels of fructose and can be used as part of a healthy diet since the consumption of fructose-rich
foods is recommended. *Acknowledgment: Financial support for this study was provided by the
Scientific and Technological Research Council of Turkey (TUBITAK- Project number: 115Z365).

KEYWORDS
Grape, Vitis vinifera, Sugar, Organic acid

Corresponding Author: AYNUR KURT, aynur-leyla@hotmail.com

1
KARADENZ TECHNCAL UNVERSTY
2
ERZNCAN UNVERSTY

1348
 Poster Session 10
Submission ID: 1299

HERBAL NUTRACEUTICALS

1 1 1
K.BURCU BYKKILI , M. SERDAR AKIN , BRA GNC

ABSTRACT
Nutraceutical, a portmanteau of the words nutrient and pharmaceutical, was coined by
Stephen DeFelice, who defined nutraceuticals as foods (or part of a food) that provide medical or
health benefits, including the prevention and/or treatment of a disease. The European Nutraceutical
Association (ENA) defines nutraceuticals as substances that markedly contrast pharmaceuticals, which
are synthetic substances or chemical compounds formulated for specific indications. Nutraceuticals
are, hence, nutritional products that provide health and medical benefits, including the prevention and
treatment of disease. There are numerous classifications and categorizations of nutraceuticals,
functional foods and dietary supplements. In the case of nutraceuticals and functional foods, however,
consumer acceptance is considered to be overwhelmingly positive throughout the US, Canada, Europe
and Japan, though Europeans tend to be more critical of new food products and technologies.
Functional foods and beverages for cholesterol and diabetes, cognitive functions, are those related to
the digestive system. Most common nutraceuticals are dietary support products, minerals and
vitamins, protein and herbal support. Another type of compounds commonly referred to as
nutraceuticals which also present a nutritional value are pre- and probiotics. Tea, rosemary, soybean,
flaxseed, yellow centaur, tomato, garlic, ginkgo, echinacea, ginseng, dietary fiber are some important
herbal nutraceuticals. The effective components of tomato, grape seed, green tea and soybeans are
lycopene, resveratrol, EGCG (Epigallocatechin gallate) and isoflavones, respectively, which are
considered as nutraceuticals. Herbal nutraceuticals can be used as food supplements.

KEYWORDS
Nutraceuticals, herbal nutraceuticals, effective components

Corresponding Author: K.BURCU BYKKILI, brcbykklc@gmail.com

1
HARRAN UNVERSTY, FACULTY OF AGRCULTURE DEPT. OF FOOD ENGNEERNG, ANLIURFA, TURKEY

1349
 Poster Session 10
Submission ID: 1300

THE INVESTIGATION OF SOME YIELD AND YIELD COMPONENTS

OF POPPY (PAPAVER SOMNIFERUM L.) CULTIVARS GROWN IN
YOZGAT ECOLOGICAL CONDITIONS

1 2 2 3 1
HLYA DOAN , BELGIN COGE ENKAL , CNEYT CESUR , TANZER ERYILMAZ , SELEN ALNIAK SEZER ,
3 2 2
MURAT KADIR YELYURT , TANSU USKUTOLU , CENNET YAMAN

ABSTRACT
Poppy (Papaver somniferum L.), which is a member of the Papaveraceae, this is both a
medicinal and edible oil plant. There are 5 genus in Turkey belonging to this family. The poppy plant
(Papaver somniferum L.) is been the purple and white flowers, with a closed capsule, annual, and
herbaceous plant with a length of 60-200 cm. Turkey which import oil seed sand crude oils every year
for the Exchange pay sout over 1,5 billion dolar is a net importer of the oil seed sand crude oils. There
is a great vegetable oil in our country and the amount of imported 755 thousand tons of raw vegetable
oil per year is 1542 thousand tons imported from abroad. With increased demand for oil and vegetable
oil for fuel needed in our country, increased the production of oil seeds is required. For the cultivation
of poppy, Turkey has a very favorable climatic and soil conditions. Seed and oil yield, fatty acid
composition and oil fuel related characteristics, which areaffected by variety and environmental
conditions. In this study, registered in our country 2 poppy varieties (Ofis-8 ve TMO-1) aimed to
determine in Yozgat ecological conditions the morphological characteristics such as plant height,
capsule yield per plant, capsule width, capsule length, capsule yield, seed yield and weight of 1000
seeds and oil ratio in varieties were examined. The highest yield in this study, carried out between
2015 and 2016, was obtained from TMO-1.

KEYWORDS
Poppy, variety, Yozgat, yield, oil rate

Corresponding Author: SELEN ALNIAK SEZER, selenalniak@gmail.com

1
BOZOK NVERSTES TEKNK BLMLER MESLEK YKSEKOKULU
2
BOZOK NVERSTES ZRAAT FAKLTES
3
BOZOK NVERSTES MHENDSLK MMARLIK FAKLTES

1350
 Poster Session 10
Submission ID: 1303

USE OF GOJI BERRY AS FUNCTIONAL FOOD

1 1 1
K.BURCU BYKKILI , MUTLU B. AKIN , ASLI ELKEL

ABSTRACT
Goji berry is a perennial woody plant belonging to the family Goji berry solanaceae and has
two species, Lycium barbarum L. and Lycium chinense L.. These are the two closest color box types
found in Solanaceae, in the abstract family. The family includes potatoes, tomatoes, eggplant,
belladonna, pepper and tobacco. Originated from the Asian continent, this plant is now produced in
China's Tibetan region. Goji berry (Wolfberry, Lycium barbarum) grows about 1-3 meters in length.
Goji berry is 1-2 cm long, bright orange-red elliptical fruit. The Goji berry contains 68% carbohydrate,
12% protein, 10% lipid and 10% fiber. Vitamins and minerals are also rich in Goji berry. It contains
beta-sitosterol, Vitamin B complex, Vitamin E, Vitamin C, Zeaksatin, Carotenoids, Beta-sitosterol,
Cyperone, Solavetivone, Physalin, Betaine, and a large number of phenolic acids and. Lycium
barbarum (Goji fruit or wolfberries, Solanaceae family) is a chemical rich resource with health
promoting properties, Goji berry, used in liver, kidney and eye treatment in traditional Chinese
medicine, has become very popular over the past few years with its nutritional value and content. As a
result of the clinical studies, there was a decrease in the complaints of goji berry patients neurological /
psychological features, joint / muscle functions, sleep quality, ability activities, gastrointestinal system
problems, fatigue, headache, depression, diabetes, glucoma, impaired concentration, memory loss and
shortness of breath. These recent studies show that Goji Berry has anti-aging, anti-myelosuppression,
pro-apoptotic, anti-tumor, neuroprotective effect, immunomodulation, blood sugar and serum lipid
lowering effects besides antioxidant effect. While Goji berry offers many benefits and advantages in
terms of health, it can have some side effects depending on the person's health conditions and
consumption pattern. Goji fruit is harmful to diabetes patients because it affects the functioning of the
pancreas and the production of insulin. Goji berry, blood thinner like warfarin, prevents blood clotting.
It can negatively affect the body's blood pressure levels and cause hypertension. Excessive
consumption can lead to severe side effects such as dizziness, eye disturbance, blurred vision and
hallucinations. Since the beginning of the 21st century, interest in wolfberries has increased due to
innovation and the value of the foreseen food. Goji berry plant can be widely consumed in the form of
dried fruit, marmalade or fruit juice. Goji berry can be used as functional food because of its high
antioxidant and phenolic content.

KEYWORDS
Goji Berry, composition, health benefits, functional food

Corresponding Author: K.BURCU BYKKILI, brcbykklc@gmail.com

1
1HARRAN UNVERSTY, FACULTY OF AGRCULTURE DEPT. OF FOOD ENGNEERNG, ANLIURFA,
TURKEY

1351
 Poster Session 10
Submission ID: 1304

POLYAMINES AND PHENOLIC COMPOUNDS CONTENT IN THE

FRUIT OF NATURALLY GROWING MALUS SLYVESTRIS L. IN
GMHANE (TURKEY)*

1 1 1 1 1
AYNUR KURT , NESRIN COLAK , AHMET COLAK , YAKUP KOLCUOGLU , FAIK AHMET AYAZ

ABSTRACT
Aim of the study: Antioxidants are disease-fighting compounds that help prevent and repair
oxidation damage. Phenolic compounds and polyamines also exhibit various therapeutic effects (such
as diuretic properties, toxin removal and reducing the risk of cancer). Apple is in great demand by
consumers in all countries due to its high phenolic and polyamine content. The purpose of this study
was to determine the polyamines and total phenolics of a wild type of increasingly cultivated apple
(locally known as the
Methods: Apple samples of the same maturity to be used in analyses were collected from different
habitats in Gmhane. These were treated with liquid nitrogen and then stored at -80 C until
analysis. Standard methods of analysis were used to determine polyamine and phenolic contents in the
peel and flesh. Results: The polyamine (nmol per 1 g fw) with the highest content in the Gmhane
apple was spermidine in the peel and flesh (188.27 and 79.45, respectively), while putrescine
represented the least abundant polyamine in the fruit parts (35.17 and 21.61, respectively). The total
phenolic compounds and flavonoid contents in the fruit evaluated were 3267.66 and 200.94 mg 100 g-
1 in peel and 1614.66 and 14.24 mg 100 g-1 of fw in the flesh. Apple peel exhibited higher antioxidant
activity (M Trolox equivalents (TE)/g fw) than the flesh; antioxidant capacity values in peel were
0.37 for DPPH and 22.51 for FRAP. In conclusion, the apple cv that grows naturally in Gmhane
and that is also increasingly widely cultivated has high polyamine and phenolic contents. It may also
be beneficial to human health and have good commercial potential as a promising antioxidant for use
in food preparation. *Acknowledgment: Financial support for this study was provided by the Research
Fund of Karadeniz Technical University (KTU-BAP Project No: 6443).

KEYWORDS
Apple, Malus sylvestris, Phenolics, Polyamines

Corresponding Author: AYNUR KURT, aynur-leyla@hotmail.com

1
KARADENZ TECHNCAL UNVERSTY

1352
 Poster Session 10
Submission ID: 1305

ANTIMICROBIAL ACTIVITY OF POMEGRANATE PEEL

EXSTRACTS

1 1 2
AIE NL , ULE AHN N , SAIM OK

ABSTRACT
Mastitis is an intramammary infectious disease that is common in dairy cattle farming all over
the world and causes major economic losses in the dairy industry. Staphylococcus aureus is one of the
most prevalent contagious pathogens causing clinical and subclinical bovine mastitis [1, 2]. S. aureus
has a great ability to acquire resistance to antibiotics, in particular to methicillin. Methicillin-resistant
Staphylococcus aureus (MRSA) is a pathogen emerging in community and livestock as well as
hospitals [3]. Even though pharmacological industries have produced a number of new synthetic
antibiotics in the last three decades, antibiotic resistance of microorganisms against these drugs has
also increased [4]. There has been an increasing interest in recent years on antimicrobial compounds
obtained from natural sources that are not toxic and with lesser costs rather than developing new
synthetic antibiotics to combat the disease. The use of plant extracts and phytochemicals, both with
known antimicrobial properties, can be of great significance in therapeutic treatments in the last few
years. Pomegranate is a good natural resource of pharmaceutical, nutraceutical and biologically active
ingredients showing high antioxidant properties as well. Pomegranate peel methanol extract contains
high concentration of tannins, like punicalin and punicalagin, elagic acid and gallic acid showing
antioxidant activity [5] . In the current contribution, phenolic compounds of pomegranate peel were
obtained using alcoholic and hydroalcoholic extractions. The antimicrobial effect of these extracts
against five S.aureus strains, isolated from subclinical bovine mastitis, was also investigated using
agar well-diffusion method and minimum inhibition concentration. It has been observed that the
pomegranate peel extracts have antimicrobial effect on all microorganisms, giving inhibition zones
ranging in size from 14 mm to 22 mm. The MIC values for active pomegranate extracts ranged
between 0.0625-0.25 mg/ml. The current results show that pomegranate peel extracts have a potential
to be used as an antimicrobial agent against MRSA. References: 1. YANG, F., et al., Penicillin-
resistant characterization of Staphylococcus aureus isolated from bovine mastitis in Gansu, China.
Journal of Integrative Agriculture, 2017. 16(2): p. 60345-7. 2. Marques, V.F., et al., Biofilm
production and beta-lactamic resistance in Brazilian Staphylococcus aureus isolates from bovine
mastitis. Brazilian Journal of Microbiology, 2017. 48(1): p. 118-124. 3. Sowash, M.G. and A.-C.
Uhlemann, Community-associated methicillin-resistant Staphylococcus aureus case studies.
Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols, 2014: p. 25-69. 4. Thenmozhi, M.
and S. Rajeshwari, Phytochemical analysis and antimicrobial activity of Polyalthia longifolia.
International Journal of Pharma and Bio Sciences, 2010. 1(3): p. 1-7. 5. Howell, A.B. and D.H.
D'Souza, The pomegranate: effects on bacteria and viruses that influence human health. Evidence-
Based Complementary and Alternative Medicine, 2013. 2013.

Corresponding Author: AE NL, aakpinar@gtu.edu.tr

1
GEBZE TEKNK NVERSTES
2
GOLDEN JUCE OLVE OL COMPANY

1353
KEYWORDS
Pomegranate, Subclinical mastitis, S.aureus, MRSA, Antimicrobial agent

1354
 Poster Session 10
Submission ID: 1307

ANTIMICROBIAL ACTIVITY OF MEDICINAL MACROFUNGI

1 1 1
LYE DUDU GL , ZLEM CODAN , MERVE ERYILMAZ

ABSTRACT
Today, antimicrobial agents are commonly used against infections but the frequently usage of
these substances caused that pathogens gain resistance to them and reduced the effect of these
substances. In order to come over this problem, most of the researchers try to find new organisms that
produce antimicrobial agents. Until today some studies have done about medicinal plants showing
antimicrobial activity. Some fungal strains are investigated that produce antimicrobal agents. One
study reported that the ethyl acetate obtained from extracts of macrofungi called Russula delica had
antimicrobial activity on Corynebacterium xerosis. Another study showed that Reishi extracts had
antimicrobial activity on fish pathogens called Listonella anguillarum and Yersinia ruckei. Another
study investigated that the essential lipid chemical compourd called Levofloxaci obtained from dried
misels of G. japanicum fungi showed antimicrobial activity on methicilin resistant straints of S. aureus
and E. coli. The results of these studies showed that some of the macrofungi produced agents showing
antibacterial, antiviral, antifungal vs. activity. The agents obtained from macrofungi are useful for
human to treat infections. The aim of this study is to examine the fungi that showed antimicrobial
activity in literature. The results obtained this study showed that there is not enough study on this
subject. New researches are needed for future about antimicrobial activity of macrofungi.

KEYWORDS
Antimicrobial activity, Macrofungi, Pathogen

Corresponding Author: LYE DUDU GL, ulkuyedudugul@gmail.com

1
BLECK EYH EDEBAL NVERSTES

1355
 Poster Session 10
Submission ID: 1308

POLLEN MORPHOLOGY OF NATURAL DISTRIBUTED TWO

DAUCUS (APIACEAE) TAXA IN TURKEY

1 1 1
SMHAN POTOLU ERKARA , ONUR KOYUNCU , FILIZ SAVAROLU

ABSTRACT
This study aims to find out palynological properties of Daucus broteri Ten. and Daucus
guttatus that are grown in Osmaneli (Bilecik). For palynological analysis of plant materials, pollen
preparations of each taxon were prepared for light microscopy research according to the Wodehouse
and Erdtman methods and measurement of the morphological characters of pollen were carried out.
Pollen grains of each taxon ,which adhesive on the stap mounted directly, were also taken
microphotography in Scanning Electron Microscope (SEM) for detailed exine ornamentations. It was
revealed that the pollen of two Daucus taxa was infratectate, tricolporate type, prolate shaped and
regulate ornamentation. In addition to the systematic features of the taxa pollen morphologies is also
distinctive. It was also revealed that this study will help to sort out phylogenetic relationships of
between studied taxa.

KEYWORDS
Daucus, Apiaceae, Pollen Morphology, Light Microscope, SEM, Turkey.

Corresponding Author: SMHAN POTOLU ERKARA, ismuhan@ogu.edu.tr

1
ESKEHR OSMANGAZ NVERSTES, FEN EDEBYAT FAKLTES, BYOLOJ BLM, ESKEHR

1356
 Poster Session 10
Submission ID: 1309

ANTIOXIDANT AND ANTIMICROBIAL ACTIVITY OF MESPILUS

GERMANICA (L).

1 2 2
ELF GLBAHE MUTLU , HATCE TANER SARAOLU , NURSENA ARSLAN, EMNE ARSLAN , GKHAN
2
ZENGN

ABSTRACT
Mespilus germanica L. which has an edible fruit is a medicinal plant whose therapeutic effects
have historically been emphasized. In this study, we determined antioxidant capacities of methanolic
extracts obtained from M. germanica fruits and seeds by using DPPH assay and Folin-Ciocaltaeu
method. It was revealed that the total phenolic contents of methanolic extracts of M. germanica seeds
(22.94 mgGAE/g extract) were higher than M. germanica fruits (4.67 mgGAE/g extract). Also, the
seed extract (9.33 mgTE/g extract) exhibited stronger free radical scavenging activity in DPPH assay
as compared to fruit extract (6.67 mgTE/g extract). Moreover, we determined antimicrobial activity of
methanolic extract obtained from M. germanica fruits and seeds against 16 different bacteria by the
microdilution method. While the extract of M. germanica obtained from seeds used in study did not
have any effects againts bacteria, it was shown that the fruit extract was effective againts five bacteria
in 32 mg/ml concentration and against eleven bacteria in 64 mg/ml concentration. Our findings could
provide a starting point for further studies on M. germanica.

KEYWORDS
Mespilus germanica, Antioxidant, Antimicrobial, Free Radical Scavenging, Phenolic
Contents.

Corresponding Author: ELF GLBAHE MUTLU, elif.mutlu@karatay.edu.tr

1
KTO KARATAY NVERSTES
2
SELUK NVERSTES

1357
 Poster Session 10
Submission ID: 1311

ANTIMICROBIAL EFFECTS OF THYME OLEORESIN AGAINST B.

COAGULANS

1 1
AHSEN RAYMAN ERGN , TANER BAYSAL

ABSTRACT
Oleoresins (OR) are defined as a mixture of oil and resin extracted from different plants
containing essential oil. Oleoresin has characteristics flavor and aroma of spices which are the same as
the original. Oleoresin extraction is generally done with organic solvents, such as ethylene dichloride,
acetone, ethanol, methanol, hexane, ether and isopropyl alcohol. Rosemary, garlic, ginger, thyme,
basil, cumin, dill, mustard, coconut, black pepper, red pepper, celery, clove, cocoa and coriander are
among the commercial oleoresins. Oleoresins and essential oils are used in processed meat, fish,
vegetables, sodas, sauces, cheeses and other dairy products, floury foods, candy, snacks and
beverages. Natural essential oils and oleoresins have antimicrobial and antioxidant properties and are
effective against microorganisms that cause food poisoning. Sauces are a permanent food media for
fortification with all three of these plant materials or oils/oleoresins of these spices. Tomato sauce is
an acidic product and is under risk of B. coagulans because spores of B. coagulans are able to
germinate and grow at pH values as low as 4. This is an acidophilic and thermotolerant spoilage
bacterium which causes foods to go flat and sour and is particularly reported for tomato based
products. There is a lack of information in literature about the influence of these spice oleoresins and
there are limited studies about the effects of the essential oil forms of these spices on this bacteria.
Thyme (Thymus vulgaris L.) was preferred for providing the sensory properties of foods and also its
antimicrobial effects are well known. In addition the antioxidant and antibacterial activities of thyme
essential oil and oleoresin also have been previously mentioned several times. For this reason in this
study, the antimicrobial activity of thyme (Thymus serpyllum) oleoresin against Bacillus coagulans in
a tomato based sauce was investigated. The inhibitory and bactericidal effect of oleoresin against
B.coagulans ATCC 7050 was tested in the sauce media (pH 4.2 and Brix 10). As a result, thymol as a
major component of thyme oleoresin similar to its essential oil was identified with gas
chromatography-mass spectrometry (GC-MS). Minumum inhibition concentration (MIC) was found
as 2.5 ml/100 ml sauce for the thyme. In addition at the the end of 8 hours, with thyme 3.20 log
reduction was provided.

KEYWORDS
Oleoresin, thyme, essential oils, tomato sauce, MIC (Minumum Inhibition concentration), B.
coagulans

Corresponding Author: AHSEN RAYMAN ERGN, ahsenrayman@hotmail.com

1
EGE NVERSTES

1358
 Poster Session 10
Submission ID: 1312

SOME MEDICINAL FEATURES OF SPIROGYRA MAJUSCULA AND

ZYGNEMA PECTINATUM

1 1 2
KKSAL PABUCU , DERYA BAYER , TUBA DEMRZ YCER

ABSTRACT
In this study, some medicinal features of Zygnema pectinatum and Spirogyra majuscula were
investigated. Vitamin A, E capacity and antioxidant properties of Z. pectinatum and S. majuscula
isolated from Ataky Dam Lake (Tokat) were examined by HPLC and spectrophotometer. As a result
of the research, Z. pectinatum was found to be richer than S. majuscula in terms of the amount of
vitamins (A, E) and antioxidant properties.

KEYWORDS
Zygnema pectinatum, Spirogyra majuscula, Chlorophyta, Vitamins, Antioxidant Properties

Corresponding Author: KKSAL PABUCU, koksal.pabuccu@gop.edu.tr

1
GAZOSMANPAA NVERSTES
2
KARABK NVERSTES

1359
 Poster Session 10
Submission ID: 1313

THE ANTIMICROBIAL ACTIVITY OF LYNGBYA

PORPHYROSIPHONIS

1 2 3
TUBA DEMRZ YCER , KKSAL PABUCU , YAVUZ BEYATLI

ABSTRACT
In this study, the antimicrobial effects of extracts prepared with Lyngbya porphyrosiphonis
produced in culture conditions were investigated. Extracts were prepared with 0.5 M Tris-HCL pH:
8.00, N-butanol and Ethanol. Antimicrobial activity tests were performed by disc diffusion method
and the extracts were tested on microorganisms of Staphylococcus aureus ATCC 25923, Bacillus
subtilis ATCC 6633, Listeria monocytogenes ATCC 7644, Escherichia coli O 157: H7, Pseudomonas
aeruginosa ATCC 27853, Salmonella typhimurium CCM 5445 and Candida albicans ATCC 10239.
The highest antimicrobial effect was recorded against Escherichia coli. Other test microorganisms
were also affected at different levels.

KEYWORDS
Cyanobacteria, Lyngbya porphyrosiphonis, Antimicrobial activity, The Disc Diffusion Method

Corresponding Author: TUBA DEMRZ YCER, tugbayucer@karabuk.edu.tr

1
KARABK NVERSTES
2
GAZOSMANPAA NVERSTES
3
GAZ NVERSTES

1360
 Poster Session 10
Submission ID: 1314

GC-MS IDENTIFICATION OF ISOQUINOLINE ALKALOIDS IN

GLAUCIUM LEIOCARPUM NATURALLY GROWING IN CENTRAL
ANATOLIA

1 2 2 2 2
IDEM OLAK , GLEN REM KAYA , MUSTAFA ALI NR , NEHIR NVER SOMER , BUKET BOZKURT

ABSTRACT
Plants of the Papaveraceae family are distributed largely throughout north temperate and
tropical regions of the world (1). The genus Glaucium Adans. is represented by seven species among
the Papaveraceae genera found in Turkey and as known boynuzlu gelincik (horned poppy).
Glaucium leiocarpum Boiss. is the most common species of the genus Glaucium in Anatolia (2).
Isoquinolines are a widespread alkaloid class within the Magnoliales, Arostolochiales, Laurales,
Piperales, Ranunculales and Papaverales with high number of biological activities (3). Glaucine, the
most common alkaloid in Glaucium species, is used in some of the European countries in treatment,
for its antitussive effect without creating depressing impact (4). GC-MS (gas chromatography mass
spectrometry) has been proven a fast and reliable method for the investigation of the components in
complex alkaloid mixtures. The present study deals with the GC-MS analysis of alkaloids in the aerial
parts of Glaucium leiocarpum collected from Akehir, Konya. The alkaloids were identified by
comparing their mass spectral fragmentation with standard reference spectra from the NIST MS
Search 2.0 (National Institute of Standards and Technology, Gaithersburg, MD, USA), or by GC/MS
co-chromatography with previously isolated authentic standards. Moreover, data obtained from the
literature were used for the identification of the alkaloids. Totally, nine isoquinoline type alkaloids
were detected in the alkaloid extract prepared from Glaucium leiocarpum. Glaucine was found as a
major constituent (% 78,42). Dehydroglaucine (% 10,83) and allocryptopine (% 4,37) were also
detected as major components. Norchelidonine, N-methyllaurotetanine and isocorydine are other
important compounds found among the constituents. Acknowledgements This study was financially
supported by TUBTAK (Project No: 315S064) and EBILTEM (Project No: 2016/BL/002). We thank
Ege University, Faculty of Pharmacy, the Research Laboratory of Pharmaceutical Sciences (FABAL)
for facilitating GC-MS analysis. References 1. Brummitt, R. K. 1992. Vascular plant families and
genera, Royal Botanic Gardens, Kew. 2. Cullen, J. 1965. Glaucium A. in Davis P.H. (ed). Flora of
Turkey and the East Aegean Islands 1, 214, Edinburgh: University Press. 3. Bentley, K.W. 2003. Nat.
Prod. Rep. 20: 342. 4. Cortijo, J., Villagrasa, V., Pons, R., Berto, L., Marti-Cabrera, M., Martinez-
Losa, M., Domenech, T., Beleta, J., Morcillo, E. J. 1999. Brit. J. Pharmacol. 127, 1641-1651.

KEYWORDS
Glaucium, Isoquinoline alkaloids, GC-MS

Corresponding Author: BUKET BOZKURT, buket.bozkurt@ege.edu.tr

1
SALIK BLMLER NVERSTES ZMR TEPECK ETM VE ARATIRMA HASTANES
2
EGE NVERSTES ECZACILIK FAKLTES ECZACILIK MESLEK BLMLER BLM FARMAKOGNOZ
ANA BLM DALI

1361
 Poster Session 10
Submission ID: 1317

EVALUATION OF HONEYCOMB CAPPING MATERIAL FOR

APOPTOTIC, NECROTIC, AND CYTOTOXIC EFFECTS ON VARIOUS
CELL LINES

1 2 2 2
ABDULLAH MELEKOLU , SEVAL BRDANE , MUSTAFA TRK , SIYAMI KARAHAN

D
ABSTRACT

TE
The honeycomb capping material is natural protective biomaterial that are synthetized by bees
and contains various active contents. Cytotoxic, necrotic and apoptotic effects of various
concentrations of this material were evaluated on three different cell lines (L929, mouse fibroblast;
DLD-1 colon adenocarcinoma cell line and H1299, lung carcinoma cell line). At the lowest
concentration (0,0313mg/ml); the cell viability rates were 94.110.00%, 74.11 0.02%, and
EN
68.30.01% for L929, DLD and H1299, respectively. Such rates dropped downed to %30.580.01%,
20.760.02%, and 21.850.012% for L929, DLD and H1299, respectively at the highest concentration
tested (1mg/ml). The honeycomb capping material caused cell death essentially by necrosis other than
apoptosis. At the lowest concentration (0,0313mg/ml); the necrosis rates were 3.561.41, 102.21 and
ES
14.431.14 for L929, DLD and H1299, respectively. Such rates increased up to 50.331.05%,
65.371.21% and 64.510.56 for L929, DLD and H1299, respectively at the highest concentration
tested (1mg/ml). At the lowest concentration (0,0313mg/ml), the apoptotic rate was 0% for all three
cell lines that increased at a limited rate as the concentration increased. At the highest rate used
(1mg/ml), the apoptotic rates for L929, DLD and H1299 were 17.771.05%, 1.871% and
PR

4.301.23%, respectively. As a result, the natural material honeycomb capping causes necrosis on
DLD and H1299 cancel cell line, and it causes necrosis to a great deal and apoptosis to some degree
on L929 fibroblast. The valuable honeycomb capping material, secreted by bees, should be further
investigated for its effects and metabolism.
T

KEYWORDS
O

Honeycomb capping material, apoptosis, necrosis, cytotoxicity

N

Corresponding Author: SYAM KARAHAN, siyamikarahan@yahoo.com

1
STANBULNVERSTES
2
KIRIKKALE NVERSTES

1362
 Poster Session 10
Submission ID: 1318

USE OF PROBIOTIC IN INFLAMMATORY BOWEL DISEASES

1 1 1 1
HLAL DEMREZEN , TUBA KUNDAKCI , EZG TOPTA BIYIKLI , AL EMRAH BIYIKLI

ABSTRACT
USE OF PROBIOTIC IN INFLAMMATORY BOWEL DISEASES In sustaining a healthy
life, nutrition has a great importance. For healthy nutrition, beside adequate and balanced diet, there is
a need for a health digestion system. Just as any inflammation or disease in digestive tract affects
digestion of nutrients, it also impedes the occurrence of many functions. Especially chronic diseases in
digestion system affect the nutritional state of the individual in both short and long term. Inflammatory
bowel diseases, among these chronic diseases, affecting the various place in digestive tracts, show
flaming together with inflammation. Inflammatory bowel disease generally has two sorts as crohn
disease that can hold from mouth to anus, and ulcerative colitis that holds colon. In both diseases, diet
treatment has a great importance and this treatment should continue lifelong .It was seen that some
specific nutrients and supplementary can provide benefit in inflammatory bowel diseases for
treatment. In the recent years, the studies toward the effectiveness of probiotics have accelerated.
Probiotics are useful microorganisms present in intestines. It shows positive effects on human health,
modifying bowel microbiota. AIM: In this study, it was aimed to compile the studies examining the
effect of use of probiotic in inflammatory bowel diseases. METHOD: In this compilation study, with
examining the actual literature studies, the effectiveness of using probiotic in protecting from
inflammatory bowel diseases and in treatment were evaluated. RESULTS: The studies examining the
effectiveness of probiotics, known as useful basils in intestines, in treatment of inflammatory bowel
diseases frequently showed positive results. In the studies, it was generally seen that probiotic
supplementary was used instead of probiotic nutritional resources. In these studies, combining the
various strains of probiotics or using only one strain, they were given to the individuals having
inflammatory bowel diseases. In a number studies, it was observed that probiotics showed an effect
improving bowel mucosa. In many studies, carried out on ulcerative colitis, a significant decrease was
seen in remission and inflammations. In chron disease, in the studies toward the use of probiotic, it
was frequently seen that the relapse and symptoms of disease significantly decreased. In a less number
of studies, it could not be demonstrated that there was a significant effect of the use of probiotics on
inflammatory bowel diseases. CONCLUSIOON: The studies carried out showed that probiotics
frequently had the positive effects such as increasing emission, inflammation, and reducing symptoms
on inflammatory bowel diseases. For the amount of probiotic strain that are necessary to be used, just
as there is a clear suggestion, studies toward the use of natural resources of probiotics are also
insufficient . For being able to offer clear suggestions on this subject, there is a need for further
studies.

KEYWORDS
Inflammatory bowel diseases, nutrition, probiotic, crohn, ulcerative colitis.

Corresponding Author: HLAL DEMREZEN, hilaldemirezen02@gmail.com

1
SELUK NVERSTES AKEHR KADR YALLAGZ SALIK YKSEOKULU BESLENME VE DYETETK
BLM

1363
 Poster Session 10
Submission ID: 1320

PHARMACOLOGICAL SEED OILS WITH NATURAL BIOACTIVE

CONSTITUENTS: TOCOCHROMANOL PROFILING BY HIGH-
PERFORMANCE LIQUID CHROMATOGRAPHY

1 2
FATMA NUR ARSLAN , SMAL TARHAN

ABSTRACT
Recently, more attention has been focused on the utilization of pharmacological seeds and
their byproducts as well as oils with bioactive constituents. As is known, Pharmacological seed oils
are generally extracted by cold-pressing seeds with screw or hydraulic-press techniques that are able to
retain bioactive compounds such as fatty acids, phenolics, sterols or tocochromanols. The
tocochromanol homologues (-, -, - and -Ts and TTs) are the major natural bioactive constituents
of human diet and are well-known for their strong antioxidant and anti-cancer activities. As well,
numerous studies have demonstrated the potential health benefits which include hypolipidemic, anti-
atherogenic, anti-hypertensive, allergic dermatitis suppressive, nephroprotective, neuroprotective and
anti-inflammatory activities [1]. To the best of our knowledge, very little information has been
reported on the tocochromanol profile of pharmacological seed oils. Therefore, the main goal of this
study was to determine the tocochromanol profile of oils extracted by lab-scale screw-press machine
in our laboratory, from black cumin (Nigella sativa Linn.), wheat germ (Triticum vulgare), poppy
(Papaver somniferum L.), coriander (Coriandrum sativum L.), sesame (Sesamum indicum L.) and
nettle (Urtica dioica) seeds. Tocochromanol analyses were performed in these seed oils by using the
optimized high-performance liquid chromatography (HPLC) method parameters. The analyses were
performed using a Develosil C30 (2504.6 mm, 5 m; Phenomenex Inc., USA) stainless-steel column.
For optimum HPLC separation condition, mixture of eluent A (methanol: water, 99:1, v/v) and eluent
B (tert-methylbutylether: methanol: water, 80:18:2, v/v/v) with a modified gradient programme. The
results showed that the major types of tocochromanols detected in all analyzed samples were -T, -
TT, -T, -T, and -T. The highest value was observed for total tocochromanol content in wheat germ
oil sample (2590.06 mg/kg). The amount of individual isomers in wheat germ oil was as follows;
1894.44 0.36 mg/kg (-T), 582.34 0.15 mg/kg (-T), 4.63 0.01 mg/kg (-T), 24.74 0.05 mg/kg
(-TT), 81.17 0.04 mg/kg (-TT) and 2.74 0.02 mg/kg (-T). Coriander seed oil also exhibited high
amount of tocochromanol (1313.5 mg/kg) and the main homologues present in this oil were -T
(675.07 0.33 mg/kg), followed by -T (346.28 0.24 mg/kg) and -T (163.29 0.13 mg/kg). -T
occurred in highest concentrations in wheat germ, coriander seed, and nettle seed oil. Sesame and
poppy seed oils were also established to be the excellent source of -T. Black cumin seed oil contained
highest amount of tocotrienols, wherein tocotrienols were detected in levels of 98.43 0.04 mg/kg -
TT, 304.21 0.13 mg/kg -TT, and 17.61 0.03 mg/kg -TT respectively. Thus, the total
tocochromanol content ranged from 499.66 to 2590.06 mg/kg oil, significantly wider than the Codex
and similar to several literature data. References: [1] Ramesh Kumar Saini, Young-Soo Keum

Corresponding Author: FATMA NUR ARSLAN, arslanfatmanur@gmail.com

1
KARAMANOLU MEHMETBEY NVERSTES FEN FAKLTES KMYA BLM 70100 KARAMAN/
TURKIYE
2
SELUK NVERSTES FEN FAKLTES BYOKMYA BLM 42075 KONYA/ TURKIYE

1364
Tocopherols and tocotrienols in plants and their products: A review on methods of extraction,
chromatographic separation, and detection Food Research International 82 (2016) 5970.

KEYWORDS
Bioactive constituent, Tocochromanol, Pharmacological seed oil, HPLC

1365
 Poster Session 10
Submission ID: 1322

ANTIOXIDANT CAPACITIES OF TWO HALOPHYTE PLANTS

1
YAVUZ SELIM CAKMAK

ABSTRACT
Antioxidant capacities of methanol, and water extracts from Nonea caspica (Boraginaceae)
and Suaeda altissima (Chenopodiaceae) were evaluated with UV-Spectrophometer. Antioxidant
capacity were evaluated using different assay including free radical scavenging (DPPH and NO),
reducing power (ferric and cupric), phosphomolybenum and -carotene/linoleic acid bleaching. Total
phenolic and flavonoid contents were also determined. Generally, N. caspica water and methanol
extracts possess higher antioxidant activity compared to S. altissima water and methanol extracts.
These findings showed that the N. caspica and S. altissima could serve as an important natural source
of biologically active agents for using in food and pharmaceutical industry.

KEYWORDS
Halophyte plants, Biological activity, Antioxidants

Corresponding Author: YAVUZ SELM CAKMAK, yavuzselimcakmak@gmail.com

1
AKSARAY UNVERSTY

1366
 Poster Session 10
Submission ID: 1323

ANTIOXIDANT ACTIVITY, TOTAL PHENOLICS AND ANALYTICAL

CHARACTERISTICS OF SELECTED PHARMACOLOGICAL SEED
OILS

1
FATMA NUR ARSLAN

ABSTRACT
Pharmacological seed oils as a rich source of bioactive components have been used commonly
as a potential nutraceutical. They have been involved in providing health benefits such as prevention
of prostate, improvement of hypercholesterolemia, arthritis and bladder compliance, retardation of the
hypertension, decreasing of bladder and urethral pressure, and alleviation of diabetes, lowering the
levels of cancers, and possessing high-quality antioxidant potential. Due to their specific and positive
health effects, there are several studies on these oil cultivars in different regions assessing the content
of bioactive compounds. The aim of this study was to determine the basic analytical characteristics,
total phenolic compounds and free radical scavenging activities as well as the antioxidant capacities of
selected pharmacological seed oils. The oils were extracted by lab-scale screw-press machine in our
laboratory, from black cumin (Nigella sativa Linn.), wheat germ (Triticum vulgare), poppy (Papaver
somniferum L.), coriander (Coriandrum sativum L.), sesame (Sesamum indicum L.) and nettle (Urtica
dioica) seeds. A lab-scale screw press machine (15 kg seed.h-1 capacity, single head, 2hp, 1.5 kw
power) in our laboratory was used for pressing of seed samples. 40 rpm screw rotation speed and 40C
temperatures were selected as process parameters. The free fatty acid (FFA) content, peroxide value
(PV), iodine value (IV), saponification value (SV) and unsaponified matter (USM) content of seed oils
were determined according to AOCS official methods, respectively. Conjugated dien and trien
contents of seed oils were determined by measuring the specific extinction coefficients (K232 and
K270) as well as their relation or R-values (K232/K270), according to the AOCS method. The
oxidative stability index values were determined according to the Cd 12b-92 AOCS official method.
Total phenolic compounds, radical scavenging activities and antioxidant capacities were detected by
measuring the absorbance in Lambda-25 UV-Vis spectrophotometer at 765 nm, 517 nm and 695 nm,
respectively. The measurements were expressed as milligrams of gallic acid equivalents (GAEs) per
gram of samples. Physicochemical characteristics of the studied oils showed that the oils have high
quality and potential to be used as nutrient rich pharmacological oil. Besides, obtained results indicate
selected oils excellent quality, with high contents of total phenolic compounds (42.95074.230 mg
GAE/kg oil), free radical-scavenging activity values (60.01082.610 mg GAE/kg oil) and total
antioxidant capacity values (269.160520.380 mg GAE/kg oil). Thus, high content of these bioactive
compounds and physicochemical characteristics make the analyzed seed oils, nutritionally and
commercially valuable products. References: [1] Fruhwirth G.O., Wenzl T., El-Toukhy R., Wagner
F.S., Hermetter A., Fluorescence screening of antioxidant capacity in pumpkin seed oils and other
natural oils. Eur. J. Lipid Sci. Technol., 2003, 105, 266274.

Corresponding Author: FATMA NUR ARSLAN, arslanfatmanur@gmail.com

1
KARAMANOLU MEHMETBEY NVERSTES FEN FAKLTES KMYA BLM 70100 KARAMAN/
TURKIYE

1367
KEYWORDS
Antioxidant activity, phenolics, physicochemical characteristic, pharmacological seed oil,
cold pressed oil

1368
 Poster Session 10
Submission ID: 1324

BORIC ACID EFFECTS ON PHOTOSYNTHETIC ACTIVITIES IN THE

LEAVES OF SALVIA OFFICINALIS L.

1
ZLEM ARSLAN

ABSTRACT
Mediterranean basin where Salvia officinalis L. is mostly grown in, also contains toxic levels
of boron. Toxic boron content has different effects on plant physiological and biochemical processes
including disruption of growth, cell wall development, and cellular division as well as reduction in
chlorophyll contents, photosynthetic rates, and lignin contents. Salvia officinalis L. is a medicinal
plant containing several compounds with important pharmacological activity. Boron content of the soil
is very important because it leads boron accumulation in the leaves. These accumulation causes abiotic
stress in the plant and since dry leaves are drunk as herbal tea, the plant that accumulate boron would
be toxic to human. The aim of this study was to evaluate the boron toxicity accumulation in the leaves,
to compare toxicity levels and photosynthetic activity of leaves, to understand the deteriorative effects
of boron on photosynthetic pigments and to find out the boron tolerance levels of Salvia officinalis L.
In this study, photosynthetic performance and pigment contents and leaf boron content were measured
after 10 days application of 2.5 mM, 5 mM and 10 mM of boron in half strength Hoagland solution at
optimum conditions (at 25, 250 mol m-2s-1 light intensity, 16 hour light/8 hour dark, %40-50
humidity) in the controlled growth chamber. High concentrations of boron resulted in chlorosis
followed by necrosis from margin to center of leaves. Therefore, at higher concentrations the
chlorophyll pigments levels and photosynthetic capacity of the leaves decreased. Boron content of the
leaves were increased with elevated levels of toxicity. Boron accumulation in the leaves lead abiotic
stress determined by chlorosis and lower photosynthetic activity. If Salvia officinalis L. grown in
boron toxic soils, the productivity of the plant will decrease and higher accumulation of boron in the
leaves will threaten the human health.

KEYWORDS
Salvia officinalis L., Boron toxicity, Photosynthetic activity

Corresponding Author: ZLEM ARSLAN, ozlem.turan@giresun.edu.tr

1
GRESUN NVERSTES

1369
 Poster Session 10
Submission ID: 1325

NEW SOURCES OF FUNCTIONAL OF FOOD INGREDIENTS: ALGAE

1 1
TUBA EDA ARPA , HURI LYASOLU

ABSTRACT
Recently, the development of functional foods has gained much interest in the food industry
since the interest of consumers for healthy foods has been raised. Functional foods can be defined as
foods that provide one or more functional ingredients possessing health benefits as wells nutrients.
Carotenoids, polyphenols and other antioxidants, phytosterols and omega-fatty acids can be given
examples for functional ingredients. Plant foods are good source of these functional ingredients. The
natural sources are preferred to the synthetic one. Therefore, new natural sources of functional food
ingredients have been investigated. Nowadays, the possible usage of algae as potential sources of
functional ingredients has been studied. Algae have good nutritional values due to their macronutrient
and micronutrient contents. Microalgae have been reported to have a greater protein quality than
vegetable sources (wheat, rice, and legumes etc). Algae also include compounds with biological
activity. Bioactive metabolites showing cytotoxic, antitumor, nematocidal, antifungal, anti-
inflammatory and antioxidant activities have been isolated from brown algae. Algae are valuable
sources of polysaccharides referred as dietary fibers. Dietary fibers provide potential health benefits by
reducing the risk of some diseases such as colon cancer, constipation, hypercholesterolemia, obesity
and diabetes. Sulphated fucans from brown algae and carrageenans from red algae have been reported
to show antithrombotic, anti-inflammatory, antioxidant, anticancer and antidiabetic activities. Soluble
polysaccharides from algae can be utilized as prebiotic compounds. Algae include omega fatty acids
that exhibit several health benefits such as prevention from atherosclerosis, protection against
arrhythmias and reduction in blood pressure. Several different types of sterols (clinasterol, fucosterol
etc) have been isolated from algae. These sterols are known to help prevent cardiovascular diseases.
Algae are also good source of pigments (carotenoids, chlorophyll etc.). Carotenoids that show
antioxidant activities can be used as natural food colorants. Astaxanthin is a high-value carotenoid
synthesized by microalgae. Although algae provide a wide range of biologically active compounds,
the presence of toxic compounds should be checked before they are used.

KEYWORDS
Algae, Functional food, Health

Corresponding Author: TUBA EDA ARPA, tubaedaarpa@gmail.com

1
GMHANE NVERSTES BESLENME VE DYETETK BLM

1370
 Poster Session 10
Submission ID: 1326

PHARMACOLOGICAL SEED OILS WITH NATURAL BIOACTIVE

CONSTITUENTS: FATTY ACID METHYL ESTER PROFILING BY
HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

1 1
GNL AKN , FATMA NUR ARSLAN

ABSTRACT
Fatty acids (FAs) are the main bioactive constituents of naturally occurring lipids in both
animals and plants. The FAs contained in natural samples are generally composed of a mixture of
saturated and unsaturated FAs in cis- or trans- forms, with chain lengths varying from 4 to 28 carbon
atoms. The variety of chain length, degree of unsaturation, geometry, and position of double bonds,
render their composition the most descriptive characteristic of these lipids and their origin. The
biological activities of FAs also greatly depend on the number of carbon atoms, chain branching and
number, geometry and array of double bonds. Hence, the analysis of FAs profiles is of great
importance in the control of industrial products, in medical diagnostics, and in the testing of purity,
origin, or shelf life studies of food products. The main goal of this study was to determine the cis- and
trans- isomers of unsaturated fatty acid methyl esters (FAME) contained in selected cold-pressed oils
extracted by lab-scale screw-press machine in our laboratory, from black cumin (Nigella sativa Linn.),
wheat germ (Triticum vulgare), poppy (Papaver somniferum L.), coriander (Coriandrum sativum L.),
sesame (Sesamum indicum L.) and nettle (Urtica dioica) seeds. FAs of cold pressed oils were
converted to FAME derivatives before analysis, and their relative content was calculated as the % of
the total FAs. The analyses were performed by using Develosil C30 (25 cm0.46 cm, 5 m;
Phenomenex Inc., USA) column. The mobile phases were acetonitrile/water (A) and acetonitrile (B)
used through the following gradient; 025 min: acetonitrile/water and 25110 min: acetonitrile. The
optimum injection volume of samples was 25 L and FAMEs were detected spectrophotometrically at
200 nm using a photodiode array detector. Obtained results showed that FA profiles are within the
official ranges for cold pressed oils specified in the Codex Alimentarius; therefore, the results obtained
do not need any further comments. Nettle seed oil was clearly distinguished by their FA profile due to
their far greater SFA contents (40.9%) than in the other oils. It is noteworthy that all cold pressed
oils contain small amounts of SFAs, whereas PUFA range between 18.46% and 88.22%. The cold
pressed oils studied in this work also contain small amounts of trans FAs (0.03%-0.16%); and, the
content of MUFA is relatively high (7.38%-74.21%). Thus, in terms of adequacy, accuracy and
significance, proposed method appeared to be comparable to GC applications. References: [1] Lima,
E.S., Abdalla, D.S.P., 2002. High-performance liquid chromatography of fatty acids in biological
samples. Anal. Chim. Acta 465, 8191.

KEYWORDS
Fatty acid, bioactive constituent, cold-pressed oil, HPLC

Corresponding Author: GNL AKN, gnlakin@gmail.com

1
KARAMANOLU MEHMETBEY NVERSTES FEN FAKLTES KMYA BLM 70100 KARAMAN/
TURKIYE

1371
 Poster Session 10
Submission ID: 1328

INVESTIGATION OF PHYSICOCHEMICAL AND TECHNOLOGICAL

PROPERTIES OF JUJUBE (ZIZYPHUS JUJUBA) AND EVALUATION
OF ALTERNATIVE USES

1 1
MGE HENDEK ERTOP , RABIA ATASOY

ABSTRACT
Ziziphus jujuba which is also called jujube, red date, Chinese date, or Indian date is a hard-
core fruit in the Rhamnaceae family. The homeland is China. It can grow in natural environments in
our country just as it is distributed in many regions of the world. Although grown in wild
environments, it is also cultivated in gardens. It is an ancient fruit tree that grows in temperate regions
all over the world. Moreover, it is a thorny tree with yellow fragrant flowers, which are locally
harvested from its fruit. Whereas its' crumb is hard and greenish before the jujube fruit is mature, it
turns to red, black and purple color after maturation. Jujube fruit is usually dried in the sun and
consumed as a traditional snack. The leaves of the jujube plant are used as bait. The oil obtained from
the seeds of Hnnap, which is also take place in alternative medicine applications traditionally, is used
for the treatment of nerve diseases in Chinese medicine. It has been consumed as chest softener, cough
cutter, urine and phlegm remover, laxative and blood cleanser since ancient times. Moreover, It is also
used as a cholesterol and lipid lowering fruit. Due to its' potassium content, it also helps to lower high
blood pressure. The low sodium content of the fruit is a healthy choice for people who need a low salt
diet. It is rich in jujube fruit, high vitamin C and water-soluble vitamins, minerals, sugars, inorganic
and organic substances. Its antioxidant activity is high. In terms of their nutritional content, they have
an important influence on human nutrition. Fruits also contain sugar, tannin and mucilage
(exopolysaccharide) substances. In this study, basic physicochemical properties such as ash, protein
and mineral contents; technological properties such as water/oil binding capasity and grain weight and
various nutritional attributes such as antioxidant activity, phenolic content and mineral/protein
bioavailability of jujuba fruit grown in Mersin region have been determined. In view of the present
findings, except local consumption as a snack food, alternative uses have been evaluated in the food
industry.

KEYWORDS
Zizyphus jujuba, antioxidant activity, bioactivity, bioavailability

Corresponding Author: MGE HENDEK ERTOP, mugeertop@kastamonu.edu.tr

1
KASTAMONU NVERSTES MHENDSLK VE MMARLIK FAKLTES GIDA MHENDSL BLM

1372
 Poster Session 10
Submission ID: 1331

ANTIOXIDANT PROPERTIES OF CHORISPORA TENALLA AND

CAMPHOROSMA MONSPELIACA SUBSP. MONSPELIACA
HALOPHYTE PLANTS FROM TURKEY

1
YAVUZ SELIM CAKMAK

ABSTRACT
Chorispora DC. and Camphorosma L. genus commonly grown halophyte soils, and are
members of the Brassicaceae and Chenopodiaceae families, respectively. The genus Chorispora and
Camphorosma consist of about 3 and 2 species, all of which grows wild in the Turkish flora. The
objective of this study was to determine antioxidant activities of C. tenella (PALL.) DC. and
Camphorosma monspeliaca subsp. monspeliaca L. aerial part methanol and water extracts.
Antioxidant capacity were investigated using different assays including free radical scavenging
(DPPH and NO), reducing power (ferric and cupric), phosphomolybenum, and -carotene/linoleic acid
bleaching. Total phenolic and flavonoid contents were also determined. The phenolic and flavonoid
contents of C. tenella (PALL.) DC. and C. monspeliaca subsp. monspeliaca L. at a concentration of 2
mg/ml ranged between 40.38-113.77 mg GAE/g extract and 24.07-59.26 mg RE/g extract. Generally,
C. monspeliaca subsp. monspeliaca methanol extract has effective antioxidant properties compared
with other samples. The obtained results suggest that the studied samples may be considered as
valuable candidate for new functional foods and drug formulations development.

KEYWORDS
Halophyte plants, Antioxidant capacities, Brassicaceae, Chenopodiaceae

Corresponding Author: YAVUZ SELM CAKMAK, yavuzselimcakmak@gmail.com

1
AKSARAY UNVERSTY

1373
 Poster Session 10
Submission ID: 1332

THE EFFECTS OF RUTIN AND QUERCETIN ON APOPTOSIS IN 5-

FU-INDUCED HEPATOTOXICITY IN THE RATS

1 1 2
SEMIN GEDIKLI , EMIN ENGL , VOLKAN GELEN

D
ABSTRACT
Chemotherapy is a widely used form of treatment in various types of cancer. However, the use

TE
of chemotherapeutic drugs results in some unwanted side-effects and toxicity in various organs and
tissues. 5-FU is a drug used in the treatment of cancer and some studies have shown that 5-FU causes
hepatotoxicity. The Quercetin and Rutin as polyphenolic flavonoids have been shown that to help
protect tissues from diverse toxicities. The aim of this study is to investigate possible histopathological
effects of Rutin and Quercetin (Q) on apoptosis in 5-FU-induced hepatotoxicity. In the present study,
EN
48 adult Sprague Dawley male rats were randomly divided into eight groups of six rats each. The
control group rats were given intragastric (ig) corn oil (1 ml) for 21 days. The 5-FU group rats were
given ig corn oil for 21 days and 18th day injected intraperitoneally (ip) a single dose of 5-FU 50
mg/kg. Rutin50+5-FU and Rutin100+5-FU groups were given respectively ig corn oil 50 mg/kg and
ES
100 mg/kg rutin for 21 days. These groups were injected single dose of 5-FU (50 mg/kg) in the 18th
days of rutin application. Q50+5-FU and Q100+5-FU groups were given respectively ig corn oil 50
mg/kg and 100 mg/kg quercetin for 21 days. These groups were injected single dose of 5-FU (50
mg/kg) in the 18th days of quercetin application. The Rutin100 group was given rutin (100 mg/kg-ig)
the Q100 group was given quercetin (100 mg/kg-ig) for 21 days. At the end of the experiment, all rats
PR

were sacrificed and their livers were removed. Then, tissue samples were performed according to
routine histological procedure. The prepared 5-m thickness sections were stained with Crossmans
modified Mallory triple staining were evaluated for any structural changes under a light microscope.
For hepatic immunohistochemistry, Bcl-2 and Caspase-3 staining were performed. Also, the Bcl-2 and
Caspase-3 positive cell intensity were scored as follows: none = -; weak = +; moderate = ++; strong =
+++; very strong = ++++. In the Crossmans modified Mallory triple staining sections, control groups
T

liver had a normal microscopic structure. But, in the 5 FU treatment groups livers were observed
significantly degenerated hepatocytes with nuclear condensation and were seen sinusoidal dilatation
O

and an increase of connective tissue around the central vein and portal area. In Bcl-2 cell density
estimation, there was lower density in 5 FU group than control group. Also, immunopositivity of Bcl-2
N

was significantly increased in Rutin 50-5FU, Quercetin 50-5FU, Rutin 100-5FU and Quercetin 100-
5FU groups compared with 5 FU group. Intensity of Caspase-3 positivity was higher in the 5 FU
group sections compared to the control group sections. Furthermore, immunopositivity of Caspase-3
was significantly decreased in Rutin 100-5FU groups compared with Rutin 50-5FU, Quercetin 50-5FU
and Quercetin 100-5FU groups. According to our data, 5 FU can lead to apoptosis and cause evident
damage in the microscopic structure of liver. In addition, these damages can be ameliorated by
Quercetin treatment, especially Rutin treatment.

Corresponding Author: SEMN GEDKL, semingedikli@gmail.com

1
UNVERSTY OF ATATRK
2
UNVERSTY OF KAFKAS

1374
KEYWORDS
Rutin, Quercetin, 5-FU, Hepatotoxicity, Apoptosis

1375
 Poster Session 10
Submission ID: 1333

PLANT ESSENTIAL OILS USED AGAINST VARROA

1 2 1
HIDAYET TUTUN , LEVENT ALTINTA , ASIM KART

ABSTRACT
Beekeeping is always vulnerable to many sanitary factors. Varroa destructor, an obligated
ectoparasite for honey bees in beekeeping, not only causes economic loss but also causes ecological
problems related to the role of honey bees, as the most important pollinators on Earth. In this mite
control; special apiary practies, physical removal and synthetic acaricides are used. However, none of
these methods can provide a fully effective protection. Use of conventional acaricides lead to the
development of resistance to these drugs in mite, the detrimental effect on non-targeted organisms and
the residue problem in products. For this reason, the need of alternative control methods has become
compulsory in recent years. It has been known that some plant oils used widely in perfumery and food
industry for flavor and smell have been used as a repellent to certain insects. Due to this, intensive
studies have been carried out on plants with anti-varroa potential and these studies are still going on.
Recently, studies in this area have shown that vegetable essential oils such as thyme, cloves, mint,
lemon grass are lethal to some insects and fungi. It has been shown that these plant essential oils have
antifeedant, repellent, oviposition deterrent growth regulatory and anti-vector activities on the varroa.
As a result, in countries rich in biodiversity due to endemic plant species, the essential oils used in
control of this pest should be favored instead of or in combination with conventional drugs in
integrated pest management programs because of the lack of harmful effects of essential oils on non-
target organisms and environment.

KEYWORDS
Control, Essential oils, Varroa

Corresponding Author: HDAYET TUTUN, hidayettutun@hotmail.com

1
MEHMET AKF ERSOY NVERSTES VETERNER FAKLTES FARMAKOLOJ VE TOKSKOLOJ ANABLM
DALI
2
ANKARA NVERSTES VETERNER FAKLTES FARMAKOLOJ VE TOKSKOLOJ ANABLM DALI

1376
 Poster Session 10
Submission ID: 1334

LICORICE: AN OVERVIEW OF HEALTH EFFECTS

1 1
MELAHAT SEDANUR MACIT , EDA KKSAL

ABSTRACT
Wild plants and natural products have been used for centuries for health. World Health
Organization emphasized the necessity of natural products and plant derived drugs in primary health
care. Licorice (Glycyrrhiza glabra) is one of these plants, belongs to Fabaceae family, has been
consumed in traditional medicine since Prehistoric Ages. Gycyrrhiza genus has more than 30 species.
G. glabra, G. inflata and G. Uralensis are the most important licorice varities. The root and the juicy
extract is obtained by boiling the roots, are mainly consumption types of the plant. Bioavailable
components and flavor molecules involved in conical roots and rhizomes of the plant which are the
main reasons the common usage of this parts. Main bioactive component of licorice: glisirehizin is 50
times sweeter than sugar and widely use as a sweetener in different kinds of products (gums, mouth
spray, candies, health products, antacids, chewing tobacco, medicines, some alcoholic beverages and
herbal teas). Licorice is originated from Russia and China however it is also produced in
Mediterranean countries, Southeast Europe and parts of Asia. In Turkey, there is a higher consumption
of licorice sherbet in east regions especially in summer and Ramadan. There are several clinical
studies reported the positive health effects of Licorice. According to these study results: licorice has
antiulcer, antioxidan, antiinflammatory, antiatherosclerotic, antiprotozoa, antitumoral, antifungal
effects and has also positive effects on dyspepsia. Besides these, hepatorpotective effects of licorice
were also reported. As well as health benefits, licorice has been reported about some adverse effects
and interactions with cardiovascular drugs. According to case reports, long term consumption of
licorice is associated with hypertansion, hypopotasemia, muscle weakness due to its glycyrrhizin
content which inhibits 11-11 betahidroksisteroid dehidrogenaz enzyme and cause a increase in
mineralokortikoids. Studies have shown that consuming more than 95 mg/d glycyrrhizic acid causes
an increase in blood pressure. The Scientific Committee on Food (SCF) declared the upper intake of
glycyrrhizin as 100 mg/d. Licorice and its derivatives are listed at GRAS and accepted as safe by Food
Drug Administration. However, there is no data for licorice consumption for pregnant, children and
adolescents (<18 years). Guidelines for glycyrrhizic acid recommend 9.5 mg/d for an acceptable daily
intake. This corresponds to a maximum of 10-30 grams of rootstock and a maximum of half a
teaspoon per day of rootstock tea. In short, licorice has some health benefits such as antiulcer,
antioxidan, antiinflammatory, antiatherosclerotic, antiprotozoal, antitumoral, antifungal based upon its
bioactive properties. It has a wide usage of in food industry as a sweetener. However, some adverse
effects such as hypertansion and hipopotasemia anf food-drug interactions were also reported in case
presentations by the reason of higher amounts and long-term consumptions. For this reason, it is
important not to exceed its upper intake level and evaluate drug interactions.

KEYWORDS
Aromatic plants, licorice, health

Corresponding Author: MELAHAT SEDANUR MACT, sedanur.macit@gmail.com

1
GAZ NVERSTES

1377
 Poster Session 10
Submission ID: 1335

MEDICINAL USES OF SOME AROMATIC PLANTS

1 1
TUBA EDA ARPA , HURI LYASOLU

ABSTRACT
The aromatic plants have been used in the Middle East since BC because of their flavour,
preservative and medicinal properties. In both industrialized and non-industrialized countries, the
demand of aromatic plants has been increased due to their antioxidant properties. More than 150 plants
comprising of essential oils, oleoresins and natural extractives have been accepted as safe for human
consumption. Origanum (Origanum vulgare), basil (Ocimum basilicum L.), bay (Laurus nobilis L.,)
parsley (Petroselinum crispum (Mill) Mansf.), peppermint (Mentha piperita L.,) sage (Salvia
officinalis L.), rosemary (Rosmarinus officinalis L.), tarragon (Artemisia dracunculus L.) and thyme
(Thymus vulgaris L).are the examples of aromatic plants in the FDA list (FDA CFR Title 21) as
generally recognized safe (GRAS). Aromatic plants contain protein, fibre, vitamins, and minerals.
They also include phtyochemicals and essential oils possesing antioxidant and antimicrobial
properties. Phenolics are one of the major phytochemicals possesing antioxidant activities. The intake
of phenolics is associated to a lower risk of diseases such as coronary hearth disease and cancer.
Essential oils are known to be therapeuretic agents for rheumatism and dermatitis. The utilization of
aromatic plants for medicinal purposes are widespread in the Middle East. The basil, bay, oregano and
rosermary can be given as the examples of the most used aromatic plants for medicinal purposes. Basil
is used for treatment of headaches, coughs, warts, woms, constipation, bronchitis, laryngitis, tonsillitis
gastrointestinal and kindey disorder. The medicinal properties of bay leaves include the treatment of
high blood sugar, migraine, headaches, bacterial and fungal infections and gastric ulcers. Its essential
oil also is used for rheumatism and dermatitis. Origanum is used as diaphoretic, carminative,
antispasmodic, antiseptic and tonic. Rosemary is digestive and diuretic agent. It is also used externally
as antidermatosic, antipodalgic, for scurf, vaginal antiseptic, for furuncles, antiseptic, anti-infectious.

KEYWORDS
Aromatic Plants, Health, Medicinal.

Corresponding Author: TUBA EDA ARPA, tubaedaarpa@gmail.com

1
GMHANE NVERSTES BESLENME VE DYETETK BLM

1378
 Poster Session 10
Submission ID: 1336

UHPLC-MS/MS DETERMINATION OF AFLATOXINS B1, B2, G1 AND

G2 IN PUMPKIN (CUCURBITA PEPO L.) SEED OILS FROM
CENTRAL ANATOLIA REGION OF TURKEY

1 1 1 1
GNL AKN , FATMA NUR ARSLAN , KRYE NHAN KARUK ELMAS , BRAHM YILMAZ

ABSTRACT
Cucurbita pepo L. is an economically important member of Cucurbitaceae family and among
the ten leading vegetable crops worldwide with an annual product. For many years, the Cucurbita pepo
L. Seeds products have been commonly used in the food industry, are utilized in the pharmaceutical
and alternative medicine applications. Several health benefits from its regular dietary intake have been
also reported, including cancer prevention, anti-inflammation, anti-diabetic and lowering of
cholesterolemia. The determination of the characteristics is a very sensitive issue; hence, studies on the
cold pressed pumpkinseed oils from different regions highlighted its physicochemical characteristics
in respect to the oil quality. To our best knowledge, no data is available on the quality of pumpkin
seed (Cucurbita pepo L.) oils cultivated in central Anatolia regions in detail and no investigation has
been focused on the contamination of aflatoxins by UHPLC-MS/MS. Therefore, this study was aimed
to determine the content of aflatoxin contamination by using validated UHPLC-MS/MS technique, for
cold pressed pumpkin (Cucurbita pepo L.) oils cultivated in four different central Anatolia regions of
Turkey. Thermo Quan. AccMax UHPLC-MS/MS system in combination with a coupled to triple
quadrupole mass spectrometer was used for analysis. A Hypersil Gold C18 reversed phase column
(502.1 mm; 1.9 m) was used as a chromatographic column. The mobile phase consisted of (A)
ultra-pure water and (B) methanol at a flow rate of 0.4 mL.min-1. The injection volume was 20 L
and the column temperature was maintained at 25C. The extraction with AflaTestP immuno-affinity
SPE cartridge columns for aflatoxin analyses was performed according to the Yang et al. [1].
Aflatoxin standard concentrations of 125g.kg-1 in methanol were used for calibration curves. The
correlation coefficients were obtained higher than 0.9900 for each standard. Aflatoxin contaminations
were not detected in any of studied pumpkin seed oil samples. The maximum acceptable level of
aflatoxin in food materials has been regulated in many countries and the legal limits may vary from
one country to another, depending on the degree of development and economic consideration. The
food and drug administration has set a maximum acceptable level of 20 g.kg-1 for total aflatoxin in
foods for human consumption. Thus, the obtained results indicated that the cold pressed pumpkinseed
oils from central Anatolia region of Turkey in this study are safe. The findings of present study are
expected to increase the knowledge on the characteristics of these valuable medicinal seed oils and
provide as valuable contributions to better assess their potential as a source of functional oils in the
industry. References: [1] Yang, L.X., Liu, Y.P., Miao, H., Dong, B., Yang, N., Chang, F., Yang, L.,
Sun, J., Determination of aflatoxins in edible oil from markets in Hebei Province of China by liquid
chromatography-tandem mass spectrometry. Food Addit. Contam. Part B-Surveillance, 2011, 4, 244
247.

Corresponding Author: GNL AKN, gnlakin@gmail.com

1
KARAMANOLU MEHMETBEY NVERSTES FEN FAKLTES KMYA BLM 70100 KARAMAN/
TURKIYE

1379
KEYWORDS
UHPLC-MS/MS, Aflatoxin, Cucurbita pepo L., Cold-pressed oil

1380
 Poster Session 10
Submission ID: 1337

MOLECULAR ANALYSIS OF PLEUROTUS OSTREATUS (JACQ.) P.

KUMM. COLLECTED FROM KTAHYA

1 1
HAKAN ALLI , BEKIR L

ABSTRACT
Introduction: Pleurotus ostreatus is a mushroom specimen with edible and medicinal
properties and is cultivated world-wide by numerous producers. Comparing to the other widely
cultured mushroom Agaricus bisporus (J.E. Lange) Imbach, this mushroom is more resistant to
diseases and hence requires relatively less chemicals making it more healthy in that respect. It is
extensively consumed and known by the local people as istiridye; oyster, kavak; poplar or kayn;
beech. For culturing and scientific purposes, this mushroom is continuously collected from different
geographical regions in the world. In this study, we collected some Pleurotus specimens from Ktahya
region in Turkey and determined the sequences of three barcode genes, ITS, 18S and 28S for
molecular identification. Material and Methods: Genomic DNAs were isolated from the fungi samples
using Qiagen plant DNA isolation kit. ITS region was amplified by PCR using universal ITS1F and
ITS4 primers. Also, 18S and 28S gene regions were amplified using the appropriate primers. The PCR
products were excised from agarose gel and purified to homogeneity using a PCR Gel purification kit
(Fermentas). The PCR products were sequenced by Macrogen (The Netherlands). The sequences were
then analyzed in Bioedit program to obtain full contig sequences. BlastN at NCBI and Mega6 was
used to identify the closest species and construct phylogenetic trees, respectively. Results: ITS gene
sequence was analyzed by BlastN at NCBI and the closest picks were found to be P. ostreatus, P.
eryngii, P. floridanus, P. sapidus, P. spodoleucus. Also, the phylogenetic tree has shown the specimen
within the same cluster with P. ostreatus and two other species. Furthermore, analysis of 18S gene
region sequence by the primer NS6 has revealed the BlastN hits for P. ostreatus, P. cornucopiae, P.
eryngii, P.cystidiosus and P. nebrodensis. Following 28 gene sequence analysis, however, the species
of P. sapidus, P. ostreatus, P. pulmonarius, P. eryngii, P. floridanus were picked by BlastN analysis.
Discussion: Phylogenetic position of the specimen P. ostreatus was discussed in light of the results of
this study from the perspective of ITS, 18S and 28S gene sequences and that of current literature. The
confusion and information pollution in the nucleotide sequence for this mushroom was also addressed
and evaluated.

KEYWORDS
Pleurotus ostreatus, ITS, 18S, 28S gene sequence, Medicinal Mushroom, Turkey

Corresponding Author: HAKAN ALLI, hakanalli@gmail.com

1
MULA SITKI KOMAN NVERSTES FEN FAKLTES BYOLOJ BLM

1381
 Poster Session 10
Submission ID: 1338

THE EFFECT OF COMMERCIAL FOOD PAINS ON LIFE AND

DEVELOPMENT OF DROSOPHILA MELANOGASTER

1 1 1 1
MESSER TURAN , OSMAN BURAK KELEOLU , GRKEM TEYIN , EDA GNE

ABSTRACT
Food stuffs obtained by natural artificial means are additives which are generally used to
increase the visibility of foods and to eliminate negative color differences that may occur during
processing. In some studies, the effect of food colors such as Erythrocyte, Ponceu 4R, Sunset Yellow,
Tartrazin, Amarant, Karmin, Patent Blue in model organism has been determined. In this study, it was
aimed to determine the effect of the commercial food dye (0.01-0.1 mg / L) prepared at different
concentrations to the survival and development of the model organism Drosophila melanogaster
(ebony). When the rate of third stage larvae, pup and adult of insects are compared with controls, It
was observed that there was no difference in the growth period of the bug, which was fed with the
highest concentrations, and that the proportion of adults was decreased by half. According to these
results, it has been understood that the use of overdose of commercial food colors may adversely affect
the mature properties.

KEYWORDS
Drosophila melanogaster, food coloring, food additive, life-development, mature

Corresponding Author: MESSER TURAN, muesserturan@gmail.com

1
NECMETTN ERBAKAN NVERSTES

1382
 Poster Session 10
Submission ID: 1339

PU-ERH TEA: COMPONENTS AND HEALTH EFFECTS

1 1
MELAHAT SEDANUR MACIT , EDA KKSAL

ABSTRACT
Indroduction: Tea consumption is very common in the worldwide and by two-thirds of
population drink different types of teas. Teas are preferred due to their aromatic taste and several
biological effects on health based upon their antioxidant, flavanoid, theabrownin and polysaccharid
contents. There are 6 types of teas as green tea, yellow tea, dark tea (including brick tea and Pu-erh
tea), white tea, oolong tea, and black tea. Pu-erh tea is belong to dark teas and it is a kind of fermented
tea which is produced in a widepspread manner in Yunnan province of China. The indigenes consume
it as medicine, tonic, beverage, and food for energy and wellbeing. In addition to China and Southern
Asia, pu-erh tea also exist in Japan, USA, Britain. Production: In pu-erh teas production process, tea
plants (Camellia sinensis var. Assamica (L.) O. Kuntze; Theaceae) buds and leaves are exposed
heating, rolling and drying. Secondly its fermentated with microorganisms which is a high temperature
and high moisture process. Aspergillus spp. are the most widely-used of these organisms, altogether
Penicillium, Rhizomucor, Mucor, Cladosporium and Eurotium are also found in pu-erh tea. Pu-erh tea
is classified as raw and ripened pu-erh tea. In the production process, whether pu-erh tea leaves left for
several years before fermentation at the room tempereture, it defines as raw pu-erh tea. It can be stored
up to 50 years. In other case, it is held for several months and it is called ripened tea which has a
shorter manufacturing time and lower the production cost. Production process influences chemical
composition and quality in this manner aroma and taste and after all consumer acceptibility and
choice. Health effects: Pu-erh tea is associated with several health benefits. Particularly teabrownins,
the water soluble polymeric phenolic compounds derived from various polyphenols (catechins,
theaflavins, thearubigins, polysaccharides, proteins, lipids, and caffeine via microbial action give the
health protective properties to Pu-erh tea. Pu-erh tea contains higher amounts of polymeric
polyphenols due to its longer fermentation process. In a study, it was revealed the antioxidant and
hepatoprotective effects of Pu-erh tea on rats. Pu-erh tea has positive effects on steatohepatitis and
insulin resistance by enhancing IL-6/STAT3 signaling in hepatocytes, downregulating expression of
lipogenesis- and gluconeogenesis-associated genes, upregulating PPAR. In another study, it reduced
fat storage in worms by down-regulating the expression of the master fat regulator SBP-1 and
subsequently decreased fat storage. It has also anti-constipation effects in mice. Neuro-protective
effects by downregulating the transcription and translation of mGluR5. Pu-erh tea protected SH-SY5Y
cells against apoptosis induced by L-glutamate and alleviated epilepsy behaviour by inhibiting the
expression ofmGluR5. Pu-erh tea has antihiperlipidemic effects and decreases body weight by
suppressing diet-induced body fat accumulation in C57BL/6J mice by downregulating SREBP-1c. In a
study, it was also found to decrease diabetes-induced accumulation of AGEs. There is no clear report
or recommendation for the upper intake of pu-erh tea. However, in a study, 2500 mg/kg/day
administraiton of it caused disturbances in embryo-to-foetus period. Conclusion: Pu-erh tea has
become popular in recent years except its origin countries due to its attractive aroma and health

Corresponding Author: MELAHAT SEDANUR MACT, sedanur.macit@gmail.com

1
GAZ NVERSTES

1383
benefits such as antihiperlipidemic, anti-constipation, hepato-protective, neuro-protective, which was
proven by several studies however especially on animals. Even so, consumption of higher amounts
may cause health hazards. It should be emphasize to consume Pu-erh tea in relevant amounts. For this
reason, future studies are needed to specify a dosage for adverse affects if there is any.

KEYWORDS
Aromatic plants, Pu-erh tea, dark tea, health

1384
 Poster Session 10
Submission ID: 1340

IS GREEN COFFEE EFFECTIVE IN WEIGHT LOSS?

1 1
TESLIME ZGE YRSN , YASEMIN AKDEVELOLU

ABSTRACT
Green coffee is defined as raw and unroasted seeds of Coffea Rubiaceae fruits. Roasted coffee
is produced when green coffee beans are processed in several stages. Significant changes occur in the
color, texture and taste of the coffee beans due to degradation or conversion of some compounds
found in green coffee beans by roasting. The green coffee beans contain carbohydrate, lipid, protein,
chlorogenic acid (CGA), mineral, caffeine, trigonelline and free amino acids. After roasting, the
content of carbohydrates, proteins, chlorogenic acids and free amino acids in the coffee bean decrease
while the contents of lipids, minerals, caffeine and trigonellin do not change. In addition, the roasting
of the coffee beans results in melanoids resulting from the Maillard reaction of the polysaccharides,
proteins and chlorogenic acids present in the composition. With the formation of melanoids, the
content of chlorogenic acid in the roasted coffee decrease. Significant differences between the
composition of roasted coffee and green coffee arise due to this reason. Chlorogenic acids in the green
coffee composition have been of great interest, in particular the weight loss functional effect. Studies
have shown that chlorogenic acids are helpful in reducing visceral adiposity and body weight. The
primary mechanism of action of chlorogenic acid on obesity is the activation of adenosine
monophosphate activating protein kinase (AMPK), which regulates energy balance. AMPK activation
increases fatty acid oxidation in liver and skeletal muscles. In addition, chlorogenic acid enhances
hepatic expression of PPAR- (Peroxisome proliferator-activated receptor-alpha), which increases -
oxidation of fatty acids, and carnitine palmitoyl transferase 1 (CPT-1) enzyme activity. Therefore, it is
considered that chlorogenic acid will reduce lipogenesis by increasing the use of fatty acids. In the
randomized double blind study on 109 obese Japanese subjects, 297 mg CGA/185 g green coffee was
administered to the intervention group and 2 mg CGA/185 g green coffee was administered to the
control group for 12 weeks. At the end of the study body weight, body mass index (BMI) and visceral
adiposity decreased significantly in the intervention group according to the control group. In a rat
study, it was observed that for 24 weeks, CGA and caffeine intake resulted in an increase in CPT-1
activity and a decrease in lipogenesis.. Additionally, serotonin and its derivative melatonin which
decrease the appetite and increase body weight loss were detected in green and roasted coffee beans.
Studies examining the relationship between green coffee and obesity have generally used the green
coffee extract called svetol. To observe only the effects of chlorogenic acids, eliminating the effect of
caffeine, on obesity. Svetol is a decaffeinated green coffee extract standardized to contain about 45%
CGA. In the case of obese individuals, 200 mg svetol was used 5 times a day for 12 weeks, resulting
in significant reductions in body weight and body fat percentage compared to the control group. In
another study, svetol supplements (400 mg/day) have been shown to reduce body weight and increase
lean body mass in obese individuals. When the literature is examined, the consumption of green coffee
effect on weight loss by various biochemical mechanisms due to chlorogenic acids compounds.
However, caffeine amounts in green coffee is similar to roasted coffee, so it must be consumed

Corresponding Author: TESLME ZGE YRSN, tozgeyrsn@gmail.com

1
GAZ NVERSTES SALIK BLMLER FAKLTES BESLENME VE DYETETK BLM

1385
carefully. Side effects such as headache, stomach upset, nervousness, insomnia, anxiety and
arrhythmia can be seen as a result of high caffeine intake. With fewer studies done with decaffeinated
green coffee, no side effect has been found yet.

KEYWORDS
Green coffee,chlorogenic asids,weight loss

1386
 Poster Session 10
Submission ID: 1341

DISTRIBUTION OF PHENOLIC COMPOUNDS AND ANTIOXIDANT

ACTIVITY OF BUCKWHEAT (FAGOPYRUM ESCULENTUM
MENCH) SEEDS

1 2 3 2
LHAMI KARATA , NUSRET GEN , RAHIME KARATA , MAHFUZ ELMASTA

ABSTRACT
Buckwheat (Fagopyrum esculentum Moench) belongs to the family Polygonaceae is one of
the field crops, cover crop and pseudocereal. Due to significant nutritional or health-promoting
properties, it has been used both in food formulation and traditional medicine. Buckwheat is rich in
nutrients, such as protein, essential amino acids, lipids, unsaturated fatty acids, mineral, vitamins B1,
B2 and B6. The grain of this plant contains also valuable biologically active compounds especially
phenolic compounds. They are important in scavenge of reactive oxygen species as an antioxidant and
prevent the development of disease. The aim of this work was to determine the phenolic compound
distribution, total phenolic content and total flavonoid content in buckwheat seeds. The distribution of
phenolic compounds, vanilic acid, p-coumaric acid, ferulik acid, protocatechuic acid, chlorogenic acid,
catechin, rutin, quarcetin and kaempferol, were determined by high performance liquid
chromatography equipped with diode-array detection (HPLC-DAD). Antioxidant activity of the plant
seeds were assed scavenging activity test against 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical
by spectrophotometric method. Total phenolic and flavonoid contents were present at levels of 1.48
mg GAE /g DW and 0.81 mg QUA/g DW respectively in buckwheat. According to HPLC results,
individual phenolic compound contents were showed that the main phenolic compounds are rutin
(170.16 g/g DW ), vanilic acid (22.03 g/g DW ), and chlorogenic acid (44.51 g/g DW ). The other
phenolic compounds were found in low quantities. Buckwheat grains extract exhibited high DPPH
free radical scavenging activity with the average IC50 (1.5 mg seed/mL) was described as the sample
concentration that caused a decline in the initial DPPH concentration by 50%.

KEYWORDS
Buckwheat, phenolic compounds, flavonoid, HPLC, DPPH,

Corresponding Author: LHAM KARATA, ilhami.karatas@gop.edu.tr

1
GAZOSMANPAA UNVERSTY, ALMUS VOCATONAL SCHOOL TOKAT
2
GAZOSMANPAA UNVERSTY, FACULTY OF ARTS AND SCENCES, DEPARTMENT OF CHEMSTRY TOKAT
3
MDDLE BLACK SEA TRANSTONAL ZONE AGRCULTURAL RESEARCH INSTTUTE, TOKAT

1387
 Poster Session 10
Submission ID: 1343

DOES THE MYCOTOXIN OF FUSARIUM FUNGI HAVE GENOTOXIC

EFFECT IN IN VITRO?

1 1 1
SEVCAN MAMUR , FATMA NAL , DENZ YZBAIOLU

ABSTRACT
Does The Mycotoxin of Fusarium Fungi Have Genotoxic Effect in In Vitro? Sevcan
Mamur*1, Fatma nal2, Deniz Yzbaolu2 (1) Life Sciences Application and Research Center,
Gazi University, 06830, Ankara, Turkey (2) Department of Biology, Science Faculty, Gazi University,
06500, Ankara, Turkey Abstract Mycotoxins are naturally occurring toxic secondary metabolites of
fungi that may be present in food and feed. These metabolites are widely produced by fungal genera:
Fusarium, Penicillium and Aspergillus. The mycotoxins of Fusarium are mainly trichothecenes,
fumonisins, zearalenone, fusaric acid, moniliformin, fusaproliferin, fusariosin, fusarin C, equisetin,
enniatins, and beauvericin. Mycotoxins are common contaminants of many grains like wheat, corn,
barley, maize, and rice that can be contaminated in the field during harvest or storage and, can cause
economic loss. In addition, mycotoxins have nephrotoxic, hepatoxic, carcinogenic, mutagenic, and
immunosuppressive effects. These effects treat of human and animal health. For these reasons the
evaluation of mycotoxin genotoxicity is important. Nowadays, because of the increasing exposure to
mycotoxins directly or indirectly, studies have been started to determine the toxic effects of these
substances. The aim of this study is to review genotoxicity studies of Fusarium fungi in various cells
in vitro. The studies of the genotoxicity of Fusarium species including ochratoxin A, fumonisin B1,
moniliformin, beauvericin have been determined to have positive results in different cells. Contrary to
these findings, Enniatin B has not genotoxic activity. As a result, some mycotoxins have been found to
be genotoxic in certain concentrations, however this effect should also be evaluated in in vivo tests.

KEYWORDS
Fusarium fungi, mycotoxin, genotoxic effect

Corresponding Author: SEVCAN MAMUR, smamur@gazi.edu.tr

1
GAZ UNIVERSITY

1388
 Poster Session 10
Submission ID: 1344

MATHEMATICAL MODELING OF TOTAL POLYPHENOL

COMPOUND KINETICS DURING SOLIDLIQUID EXTRACTION OF
LINDEN (TILIA CORDATA) PLANT

1 1
DOANAY YKSEL , AHMET LEVENT NAN

ABSTRACT
Different plants and herbs were relied on as the only real medicine that people could rely on
for years. Today, modern medicine has overtaken most of them and has managed to solve many
health-related problems. While natural remedies certainly can't replace modern medical procedures,
they can still help in a variety of ways. Mild health problems can often be relieved through their use
and many other problems can be prevented when these remedies are used as preventatives. The
flowers can be used to make herbal tea which is used to treat anxiety since the herb exerts a soothing
effect on the body, helping calm the nerves and treat sleep disorders as well. There are several
different subspecies of linden that can be used medicinally and all may have a slightly different taste.
Linden tea is one of the most effective natural remedies and has been used for centuries. When it is
administered regularly, it can help with a wide range of different health issues. Since plants of Tilia
(Tiliaceae) genus show both antioxidant and antimicrobial activities, such functional properties are
associated with polyphenolic compounds, including flavonoids. Therefore, time-temperature relation
is important in the infusion of linden, which is rich in total phenolic compounds. In this study, the
transfer times of polyphenols to liquid phase in solid-liquid extraction of linden plants that were
brewed at different temperatures were investigated and kinetic modeling was carried out. linden plant
(Tilia cordata) tea was prepared according to the conventional method. The dry linden leaves were
milled and passed through a 3 mm sieve. The ground linden leaves placed on the steel brewing screen
were immersed in deionized water at different 5 constant temperatures. A constant and low speed
rotating magnetic stirrer was used for homogenized linden extraction. A sample of 5 ml extract was
withdrawn at different time intervals and filtered. Extraction process was carried out until the
extraction concentration at each temperature was not changed. The total phenolic compound of the
sample was determined by spectrometric method and was expressed as gallic acid equivalents (GAE)
g/L. Using differential method, the kinetics of solid-liquid extraction of total phenolic compounds
were determined. The mean equilibrium concentration of the experimental total phenolic compound in
the liquid phase at 60, 70, 80, 90 and 100 C was 41 g / L. The significant rate changes were observed
depending on the temperature at the transfers from the solid phase to liquid phase. The best function
models giving the mathematical equations of the concentrations-time changes of the of the total
phenolic substance in the liquid phase at these temperatures are exponential rise to maximum; simple
exponent, 3 parameters (y= y0 +a(1-bx)). The coefficients of determination of these functions (R2)
were found between 0.9910-0.9829.

Corresponding Author: AHMET LEVENT NAN, linanc@ksu.edu.tr

1
KAHRAMANMARA ST MAM NVERSTES

1389
KEYWORDS
Tilia Cordata, kinetic, total polyphenol, extraction

1390