Professional Documents
Culture Documents
materials requiring sterile conditions poses complex antibodies and viruses, insect cell culture and cGMP
and seemingly contradictory demands on mixing manufacturing. Scales range from bench-top systems
technology. Equipment should avoid threats of con- as small as 100-ml to production-scale units of 500-l
tamination while affording easy cleaning and simple working volume.
set-up and minimizing downtime for validation However, the bag is quite complex due to the
and process engineering. In addition, mixers should need for aeration, inoculation, sampling and acid/base
provide high output from a small footprint. addition. So GE now has launched a simpler single-
Vendors are meeting these challenges in a variety use bag for basic liquid mixing applications. The M
of ways. A couple are particularly focusing on single- Bag does away with the need for a mixing tank or a
use, i.e., disposable, mixing systems, which represent conventional mixer and, like the Wave Bioreactor, also
one of the biggest growth areas. obviates cleaning, sterilization and validation. The bags,
For instance, GE Healthcare, Chalfont St. Giles, which range in size from 20-l to 600-l, come with a
U.K. and Piscataway, N.J., touts its Wave Bioreactor large screw-cap port for the easy addition of powders
single-use system as virtually eliminating the need for and other solids, as well as fill and sample lines. They
cleaning and validation while avoiding cross-contami- also can be customized to suit an end users individual
nation (www.ChemicalProcessing.com/articles/2006 processes. If larger-scale more-aggressive mixing is
/017.html). required, GE offers versions with a built-in impeller.
Materials to be mixed only contact a pre-sterilized Demand also is growing for more sensing tech-
disposable bag mounted on a special rocking platform nologies.The company already offers dissolved oxygen,
(Figure 1). Its motion induces waves in the fluid that pH, carbon dioxide and oxygen sensors via miniature
provide mixing and gas transfer. fiber-optic microprobes. The ultimate goal here
The technology is used extensively in fermentation would be to integrate every probe that you might want