R-Biopharm AG

Real-time PCR beer screening

For a quick and GEN-IAL result

Avoid product recalls

Minimize production losses
Prevent damage to brand image
For in-process control and/or for online final product control

R-Biopharm for the safety of your analysis.

R-Biopharm AG

With real-time PCR faster results than classical microbiology

Use different analytical modules to establish your customized monitoring of microbiological beer
spoilage organisms in beer and beer products. Either extremely fast or maximal sensitive detection
and specific identification is possible by combining different methods of sample preparation and
detection with real-time PCR.

Approaches for microbiological analysis in beer

Time requirement: 3 - 14 days

Classical microbiology
Up to two weeks inubation

+ Well establisehd Slow:

Cheap Long quarantine time for
products
Uncertain, risk of wrong results
Might be expensive in consequence

Time requirement: 2 hrs

Filtration and qPCR New!

Filtration, qPCR: 2 hours from sample to result
+ Extremely fast Volume limited to approx. 1 liter
Screening and/or identification Sensitivity limited to approx.
Only two hours to result 100 cfus

Fast screening approach for specific applications

Time requirement: 50 hrs

Enrichment and qPCR

Pre-enrichment and qPCR: 2 days and 2 hours from sample to result

+ Fast Sample volume is limiting

Screening and/or identification factor for sensitivity
Ensures absence of low
cfu/sample volume
Modern establisghed approach for beverage analytic

Time requirement: 50 hrs

Polymer binding and qPCR New!

Sampling via polymer, pre-enrichment and qPCR

+ Highest sensitivity Sample volume is limiting

Up to 30 liter sample adsorbtion: factor for sensitivity
kegs, in-process control, several
bottles
Pre-enrichment of polymer ensures
absence of few cfu/sample volume

Very sensitive approach for highest demands

 Beer spoilage detection by GEN-IAL qPCR
The broadest spectrum of customized analytical solutions
1 DNA preparation
Simplex Easy DNA extraction kit
Fast and easy thermal DNA isolation in one reaction
vessel. No risk of contamination, well suitable for
pre-enriched beverage samples, centrifuge and
heating block needed.
For difficult samples as worth or for for automation
the First beer magnetic DNA kit may be used.

2 Screening
Screening kits for different qPCR thermocyclers allow
group specific or general detection of beer spoilage
microorganisms:
Lactobacillus spp. / Pediococcus spp.
Meagasphaera / Pectinatus
Yeasts
Internal Amplification Control

3 Species identification
Species specific assays for confirmation and specific identification

4 Screening and identification panel New!

+ Open platform for several blockcylcers:
Roche LC480, Agilent Mx3005P, ABI 7500,
Biorad CFX96
Prefilled strip with 8 x 3 reactions, just add
Premix and DNA
Screening and identification of most relevant
beer spoilers in one step
Other tests may be used on same qPCR
blockcycler

Most modern open system for screening and differentiation:

Well FAM HEX ROX

1 NTC NTC IAC
2 Enterobacteriacae P. anomala S. diastaticus
3 P. damnosus P. acidilactici/pentosaceus/parvulus/ P. clausenii
inopinatus
4 Pectinatus spp. Megasphaera spp. L. rossiae
5 L. brevis/L.parabrevis/L.brevisimilis L. lindneri L. casei/L.paracasei
6 L. buchneri/L. parabuchneri L. collinoides/L. paracollinoides L. perolens/L. harbinensis
7 L. plantarum/L. paraplantarum L. coryniformis IAC
8 L. acetotolerans L. backii PTC
R-Biopharm AG

GEN-IAL Products for beer analysis

Product Description No. of Tests/Amount Art. No.
Beer DNA preparation
GEN-IAL Simplex Easy DNA kit DNA preparation of beverage samples 100 preparations SE 0100
GEN-IAL QuickGEN DNA preparation of beverage samples without enrichment, 50 preparations FSE 0050
Sample preparation filtration Filtration
GEN-IAL QuickGEN DNA preparation of beverage samples without enrichment, 50 preparations CSE 0050
Sample preparation centrifugation Centrifugation
Beer Bacteria & Yeast Qualitative multiplex real-time PCR
GEN-IAL Multiplex detection (30 species) and identification 96 reactions TPBD 0096
First-Beer Differentiation PCR Kit (19 species) of relevant beer spoilers
GEN-IAL Multiplex detection (30 species) and identification 96 reactions TPBD 0096
First-Beer Differentiation PCR Kit for LC 480 (19 species) of relevant beer spoilers for LC480 LC480
GEN-IAL QuickGEN Multiplex detection (30 species) and identification 96 reactions QTPBD 0096
First-Beer Differentiation PCR Kit (19 species) of relevant beer spoilers without preenrichment
GEN-IAL QuickGEN Multiplex detection (30 species) and identification 96 reactions QTPBD 0096
First-Beer Differentiation PCR Kit for LC 480 (19 species) of relevant beer spoilers without preenrichment LC480
for LC480
GEN-IAL First-Beer yeast and bacteria Multiplex detection and identification of beverage spoiling 96 reactions TPYB 0096
differentiation TaqMan bacteria and yeasts
GEN-IAL First-Beer yeast and bacteria Multiplex detection and identification of beverage spoiling 96 reactions TPYB 0096
differentiation TaqMan LC 480 bacteria and yeasts LC480
GEN-IAL P1 Hyb Probe Screening DNA screening and differentiation of bacteria and yeasts for 50 reactions PP1H 0050
LC 2.0 FRET LC 2.0 LC2.0
GEN-IAL P1 Screening DNA Screening and differentiation of beer spoiling bacteria 50 reactions PP1T 0050
TaqMan and yeasts
GEN-IAL QuickGEN P1 Screening TaqMan DNA screening and differentiation of beer spoiling bacteria 50 reactions QPP1T 0050
and yeasts without preenrichment
GEN-IAL P1 Screening DNA screening without differentiation of bacteria and yeasts 50 reactions PP1T 0050 SP
Spartan DX-12 TaqMan
GEN-IAL P1OH Screening DNA screening without differentiation of bacteria 50 reactions PP1TOH 0050
Spartan DX-12 TaqMan SP
GEN-IAL First Yeast Hyb Probe Screening DNA screening and differentiation of yeasts 50 reactions PYHYB 0050
LC 2.0 FRET LC2.0
Beer Bacteria Qualitative real-time PCR
GEN-IAL Specific DNA detection and differentiation of Pectinatus and 50 reactions TPPMD
Pectinatus spp./Megasphaera spp. TaqMan Megasphaera
Beer resistance genes Qualitative real-time PCR
GEN-IAL hop resistance genes Specific DNA detection of hop resistance genes 50 reactions TPHR 0050
horA and horC/hitA and orf5
TaqMan
GEN-IAL QuickGEN hop resistance genes Specific DNA detection of hop resistance genes without 50 reactions QTPHR 0050
horA and horC/hitA and orf5 TaqMan preenrichment
Beer Yeast Qualitative real-time PCR
GEN-IAL Dekkera anomala TaqMan Specific DNA detection of Dekkera anomala 50 reactions TPYDA 0050
GEN-IAL Pichia anomala TaqMan Specific DNA detection of Pichia anomala 50 reactions TPYPA 0050
GEN-IAL Saccharomyces diastaticus TaqMan Specific DNA detection of Saccharomyces diastaticus 50 reactions TPYSD 0050
GEN-IAL Pichia membranaefaciens TaqMan Specific DNA detection of Pichia membranaefaciens 50 reactions TPYPM 0050
GEN-IAL Bottom fermented yeast TaqMan Specific DNA detection of bottom fermented yeast 50 reactions TPYUG 0050
GEN-IAL Top fermented yeast TaqMan Specific DNA detection of top fermented yeast 50 reactions TPYOG 0050

Further parameters are available on request.

2016/04

R-Biopharm AG An der neuen Bergstrae 17, 64297 Darmstadt, Germany E-mail: info@r-biopharm.de www.r-biopharm.com